Academic literature on the topic 'Transcript accumulation'

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Journal articles on the topic "Transcript accumulation"

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Boddu, Jayanand, Seungho Cho, Warren M. Kruger, and Gary J. Muehlbauer. "Transcriptome Analysis of the Barley-Fusarium graminearum Interaction." Molecular Plant-Microbe Interactions® 19, no. 4 (2006): 407–17. http://dx.doi.org/10.1094/mpmi-19-0407.

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Fusarium head blight (FHB) of barley (Hordeum vulgare L.) is caused by Fusarium graminearum. FHB causes yield losses and reduction in grain quality primarily due to the accumulation of trichothecene mycotoxins such as deoxynivalenol (DON). To develop an understanding of the barley-F. graminearum interaction, we examined the relationship among the infection process, DON concentration, and host transcript accumulation for 22,439 genes in spikes from the susceptible cv. Morex from 0 to 144 h after F. graminearum and water control inoculation. We detected 467 differentially accumulating barley gen
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Jia, Haiyan, Seungho Cho, and Gary J. Muehlbauer. "Transcriptome Analysis of a Wheat Near-Isogenic Line Pair Carrying Fusarium Head Blight–Resistant and –Susceptible Alleles." Molecular Plant-Microbe Interactions® 22, no. 11 (2009): 1366–78. http://dx.doi.org/10.1094/mpmi-22-11-1366.

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Fusarium head blight (FHB), caused primarily by Fusarium graminearum, decreases grain yield and quality in wheat and barley. Disease severity, deoxynivalenol (DON), fungal biomass, and transcript accumulation were examined in a wheat near-isogenic line pair carrying either the resistant or susceptible allele for the chromosome 3BS FHB-resistance quantitative trait locus (Fhb1). Fhb1 restricts spread of disease symptoms but does not provide resistance to initial infection or initial DON accumulation. Wheat exhibits both induction and repression of large sets of gene transcripts during F. gramin
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Gunning, P., E. Hardeman, R. Wade, et al. "Differential patterns of transcript accumulation during human myogenesis." Molecular and Cellular Biology 7, no. 11 (1987): 4100–4114. http://dx.doi.org/10.1128/mcb.7.11.4100.

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We evaluated the extent to which muscle-specific genes display identical patterns of mRNA accumulation during human myogenesis. Cloned satellite cells isolated from adult human skeletal muscle were expanded in culture, and RNA was isolated from low- and high-confluence cells and from fusing cultures over a 15-day time course. The accumulation of over 20 different transcripts was compared in these samples with that in fetal and adult human skeletal muscle. The expression of carbonic anhydrase 3, myoglobin, HSP83, and mRNAs encoding eight unknown proteins were examined in human myogenic cultures
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Gunning, P., E. Hardeman, R. Wade, et al. "Differential patterns of transcript accumulation during human myogenesis." Molecular and Cellular Biology 7, no. 11 (1987): 4100–4114. http://dx.doi.org/10.1128/mcb.7.11.4100-4114.1987.

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We evaluated the extent to which muscle-specific genes display identical patterns of mRNA accumulation during human myogenesis. Cloned satellite cells isolated from adult human skeletal muscle were expanded in culture, and RNA was isolated from low- and high-confluence cells and from fusing cultures over a 15-day time course. The accumulation of over 20 different transcripts was compared in these samples with that in fetal and adult human skeletal muscle. The expression of carbonic anhydrase 3, myoglobin, HSP83, and mRNAs encoding eight unknown proteins were examined in human myogenic cultures
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Kaback, D. B., and L. R. Feldberg. "Saccharomyces cerevisiae exhibits a sporulation-specific temporal pattern of transcript accumulation." Molecular and Cellular Biology 5, no. 4 (1985): 751–61. http://dx.doi.org/10.1128/mcb.5.4.751.

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Cultures of the yeast Saccharomyces cerevisiae that are heterozygous for the mating type (MATa/MAT alpha) undergo synchronous meiosis and spore formation when starved for nitrogen and supplied with a nonfermentable carbon source such as acetate. Haploid and homozygous MAT alpha/MAT alpha and MATa/MATa diploid cells incubated under the same conditions fail to undergo meiosis and are asporogenous. It has not yet been firmly established that gene expression during sporulation is controlled at the level of transcript accumulation. To examine this question, we used cloned genes that encode a variet
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Kaback, D. B., and L. R. Feldberg. "Saccharomyces cerevisiae exhibits a sporulation-specific temporal pattern of transcript accumulation." Molecular and Cellular Biology 5, no. 4 (1985): 751–61. http://dx.doi.org/10.1128/mcb.5.4.751-761.1985.

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Cultures of the yeast Saccharomyces cerevisiae that are heterozygous for the mating type (MATa/MAT alpha) undergo synchronous meiosis and spore formation when starved for nitrogen and supplied with a nonfermentable carbon source such as acetate. Haploid and homozygous MAT alpha/MAT alpha and MATa/MATa diploid cells incubated under the same conditions fail to undergo meiosis and are asporogenous. It has not yet been firmly established that gene expression during sporulation is controlled at the level of transcript accumulation. To examine this question, we used cloned genes that encode a variet
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Hall, Jennifer R., Kathy A. Clow, Matthew L. Rise, and William R. Driedzic. "Identification and validation of differentially expressed transcripts in a hepatocyte model of cold-induced glycerol production in rainbow smelt (Osmerus mordax)." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 301, no. 4 (2011): R995—R1010. http://dx.doi.org/10.1152/ajpregu.00210.2011.

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Rainbow smelt ( Osmerus mordax ) avoid freezing by producing antifreeze protein (AFP) and accumulating glycerol. Glyceroneogenesis occurs in liver via a branch in glycolysis and gluconeogenesis and is activated by low temperature. Hepatocytes were isolated from the livers of fish acclimated to 8°C. Cells were incubated at warm (8°C; nonglycerol accumulating) or cold (0.4°C; glycerol accumulating) temperature over a 72-h time course. Reciprocal suppression subtractive hybridization libraries enriched for cold-responsive transcripts were constructed at 72 h. Microarray analyses using a 16K salmo
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Raff, J. W., W. G. Whitfield, and D. M. Glover. "Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos." Development 110, no. 4 (1990): 1249–61. http://dx.doi.org/10.1242/dev.110.4.1249.

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We demonstrate that two independent mechanisms act on maternally derived cyclin B transcripts to concentrate the transcripts at the posterior pole of the Drosophila oocyte and at the cortex of the syncytial embryo. The cortical accumulation occurs because the cyclin B transcript is concentrated around nuclei and comigrates with them to the cortex. The perinuclear localisation of the transcript is blocked by inhibitors of microtubule polymerisation and the transcript colocalises with microtubular structures during the cell cycle, suggesting that the transcript is associated either directly or i
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Wang, Xiao, Sameer P. Goregaoker, and James N. Culver. "Interaction of the Tobacco Mosaic Virus Replicase Protein with a NAC Domain Transcription Factor Is Associated with the Suppression of Systemic Host Defenses." Journal of Virology 83, no. 19 (2009): 9720–30. http://dx.doi.org/10.1128/jvi.00941-09.

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ABSTRACT An interaction between the helicase domain of the Tobacco mosaic virus (TMV) 126-/183-kDa replicase protein(s) and the Arabidopsis thaliana NAC domain transcription factor ATAF2 was identified via yeast two-hybrid and in planta immunoprecipitation assays. ATAF2 is transcriptionally induced in response to TMV infection, and its overexpression significantly reduces virus accumulation. Proteasome inhibition studies suggest that ATAF2 is targeted for degradation during virus infection. The transcriptional activity of known defense-associated marker genes PR1, PR2, and PDF1.2 significantly
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Hann, Louane E., W. James Cook, Susan L. Uprichard, David M. Knipe, and Donald M. Coen. "The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation." Journal of Virology 72, no. 10 (1998): 7709–14. http://dx.doi.org/10.1128/jvi.72.10.7709-7714.1998.

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ABSTRACT Herpes simplex virus specifies two sets of transcripts from theUL24 gene, short transcripts (e.g., 1.4 kb), processed at the UL24 poly(A) site, and long transcripts (e.g., 5.6 kb), processed at the UL26 poly(A) site. The 1.4- and 5.6-kb transcripts initiate from the same promoter but are expressed with early and late kinetics, respectively. Measurements of transcript levels following actinomycin D treatment of infected cells revealed that the 1.4- and 5.6-kb UL24 transcripts have similar stabilities, consistent with UL24 transcript kinetics being regulated by differential polyadenylat
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Dissertations / Theses on the topic "Transcript accumulation"

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Finnegan, Patrick Michael. "RNA synthesis in maize mitochondria : the identification of autonomously replicating RNA species and a kinetic analysis of transcript accumulation." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75931.

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Transcription in mammalian and yeast mitochondria proceeds from a few well defined promoters, with processing of polycistronic transcripts producing the mature RNAs. The levels of different sequences in the steady-state RNA populations depend on differential promoter strengths, transcription attenuation and/or selective termination, and differential RNA stabilities. To gain insights into the processes governing transcription and RNA levels in plant mitochondria, a system using isolated maize mitochondria, which synthesize bona fide mitochondrial RNAs, was developed and partially characterized
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Swanson-Wagner, Ruth A. "Analysis of differences in gene expression and the genetic regulation of transcript accumulation in maize inbred and hybrid lines." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3369906.

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Kyriakopoulou, Garyfallia. "Molecular cloning of three different Achlya ambisexualis hsp70 cDNAs, and changes in the accumulation of hsp70 transcript populations during hyphal branching." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ35215.pdf.

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Phillips, Kyle. "Characterization of the role of Zea mays burp domain-containing genes in maize drought responses." Thesis, University of the Western Cape, 2016. http://hdl.handle.net/11394/5339.

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Philosophiae Doctor - PhD<br>Global climate change has resulted in altered rainfall patterns, causing annual losses in maize crop yield due to water deficit stress. Therefore, it is important to produce maize cultivars which are more drought-tolerant. This not an easily accomplished task as plants have a plethora of physical and biochemical adaptation methods. One such mechanism is the drought-induced expression of enzymatic and non-enzymatic proteins which assist plants to resist the effects of water deficit stress. The RD22-like protein subfamily is expressed in response to water deficit str
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Campbell, Brian Anthony. "Accumulation of BEL1-like transcripts in solanaceous species." [Ames, Iowa : Iowa State University], 2008.

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O'Hara, Paul. "The influence of fungal biotrophy on low temperature responses in winter cereals : a study, under acclimating and non-acclimating conditions, on the accumulation of transcripts from selected barley low-temperature-responsive (blt) genes, and on freezing s." Thesis, Lancaster University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322201.

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Iyengar, Anand R. "Follicle cell calmodulin: transcript accumulation in vitellogenic follicles of Blattella germanica is regulated by juvenile hormone." 1995. https://scholarworks.umass.edu/dissertations/AAI9737542.

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Calmodulin (CaM) is a major intracellular calcium receptor. There is abundant calmodulin (CaM) in the oocytes and eggs of B. germanica during vitellogenesis and early embryogenesis. The accumulation of CaM in oocytes may be for immediate use in the oocytes and/or in preparation for later stages of their development. Previous investigation from this laboratory suggested that maternal follicle cells are the most likely source of this CaM. Tissue culture labeling with $\sp{35}$S methionine showed a 13-fold higher rate of synthesis of CaM in the follicle cells than in oocyte preparations (Zhang &
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Preuten, Tobias [Verfasser]. "Organellar gene expression : regulation of phage-type RNA polymerase transcript accumulation and analyses of mitochondrial gene copy numbers in Arabidopsis / von Tobias Preuten." 2009. http://d-nb.info/1007739207/34.

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Houlahan, Nora Kathleen. "Localized post-transcriptional gene silencing of the very long chain fatty acid condensing enzyme, CER6, and analysis of transcript accumulation in the anthers of developing flower buds of Arabidopsis thaliana." Thesis, 2004. http://hdl.handle.net/2429/15669.

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In Arabidopsis thaliana, both the stem wax and the pollen lipids are generated from very long chain fatty acid (VLCFA) precursors. A mutation in the gene encoding CER6, a condensing enzyme involved in VLCFA synthesis, results in plants with alterations in both the stem wax and the pollen coat lipid profiles. Phenotypically these plants lack epicuticular wax crystals on their stems and are conditionally male sterile. When grown under conditions of low humidity, the pollen of cer6 plants does not hydrate on a receptive stigma, although hydration will occur under high humidity growth condi
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Books on the topic "Transcript accumulation"

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Kyriakopoulou, Garyfallia. Molecular cloning of three different Achlya ambisexualis hsp70 cDNAs, and changes in the accumulation of hsp70 transcript populations during hyphal branching. 1998.

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Book chapters on the topic "Transcript accumulation"

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Lodde, V., A. M. Luciano, F. Franciosi, R. Labrecque, and M. A. Sirard. "Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes." In Results and Problems in Cell Differentiation. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60855-6_11.

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"3. ACCUMULATION, REGULATION, NETWORKS." In Creative Networks and the City. transcript-Verlag, 2010. http://dx.doi.org/10.14361/transcript.9783839413746.65.

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Perrier-Groult, Emeline, Elisabeth Aubert-Foucher, Marielle Pasdeloup, Jérôme Lafont, Hugo Fabre, and Frédéric Mallein-Gerin. "Flow Cytometry Analysis of Type IIB Procollagen as Quality Control of Chondrogenic Commitment of MSCs." In Stem Cells and Regenerative Medicine. IOS Press, 2021. http://dx.doi.org/10.3233/bhr210008.

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Type II collagen is the major collagen protein in cartilage, synthesized as precursor forms (procollagens). Several splice variants of the gene encoding type II procollagen have been identified such as IIA and IIB isoforms. Interestingly, a shift from IIA to IIB transcripts has been reported to occur during cartilage development and during chondrogenic differentiation of mesenchymal stem cells in vitro. Thus, type IIB procollagen represents a reliable marker of chondrocyte differentiation. We characterized previously the first antibody (referred as anti-pNIIB52) able to selectively detect the IIB form of human type II procollagen in Western-blot or immunohistochemistry analysis. More recently, we used anti-pNIIB52 in flow cytometry to quantify chondrogenic induction of bone marrow-mesenchymal stem cells cultivated in agarose hydrogel, after release of the cells from the gel. Here, we use imaging flow cytometry and anti-pNIIB52 to visualize directly intracellular accumulation of type IIB procollagen in cells undergoing chondrogenesis. Our data together show that flow cytometry analysis using anti-pNIIB52 represents an efficient and rapid diagnostic tool of good chondrogenic conversion, at the cellular level.
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Simon, Gregory L. "Dispatches from the Field." In Flame and Fortune in the American West. University of California Press, 2016. http://dx.doi.org/10.1525/california/9780520292802.003.0009.

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This chapter uses radio communication transcripts from the Tunnel Fire to illuminate specific challenges experienced by residents and responders alike at the time of the event. Based on these first-hand accounts, several important issues emerge concerning water, road, and power infrastructure. A review of reconstruction efforts in each area of concern demonstrates that progress toward reconciliation has been mixed. Capital improvements were driven largely by private property considerations and residents seeking to leverage the disaster in pursuit of neighborhood enhancements and estate-based wealth accumulation. Upgrades to water and power line equipment were lobbied and partially paid for by residents who used their positions of privilege to engage in collectivized risk reduction. In these instances the community was willing and able to supplement beleaguered city budget capacities and help pay for municipal upgrades. This presented a win-win for residents and the city of Oakland alike. However, when private benefits were less evident (or simply not attainable)—as was the case with road-widening initiatives—residents were less apt to back such recovery efforts. As a result, the pursuit of win-win outcomes unraveled.
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Conference papers on the topic "Transcript accumulation"

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Feng, Jun-li, and Ji-shuang Chen. "Determination Suppressive Effect of Satellite 369 on Accumulation of Cucumber Mosaic Virus by Real-Time Reverse Transcript-Polymerase Chain Reaction." In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering (ICBBE '08). IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.102.

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Libby, Peter, Stephen J. C. Warner, and Louis K. Birinyi. "THE VESSEL WALL AS A SOURCE OF VASORHGOLATORY AND IMMDNOSTIMOLATORY CYTOKINES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643982.

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The cytokines Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF, also known as cachectin) exhibit multiple effects on circulating blood cells and cells of the blood vessel wall. For example, these mediators elicit a coordinated Drogram of functions of endothelial cells (EC) that promotes blood coagulation and thrombosis, and lead to clot stabilization. Furthermore, IL-1 and TNF promote adherence to vascular endothelium of leukocytes of many classes.Thus, these cytokines are likely to be involved in signaling the pathologic changes in blood vessels that characterize a number of inflammatory o
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