Academic literature on the topic 'Transcription du gène SNR6 (U6)'

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Journal articles on the topic "Transcription du gène SNR6 (U6)"

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Eschenlauer, J. B., M. W. Kaiser, V. L. Gerlach, and D. A. Brow. "Architecture of a yeast U6 RNA gene promoter." Molecular and Cellular Biology 13, no. 5 (1993): 3015–26. http://dx.doi.org/10.1128/mcb.13.5.3015.

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The promoters of vertebrate and yeast U6 small nuclear RNA genes are structurally dissimilar, although both are recognized by RNA polymerase III. Vertebrate U6 RNA genes have exclusively upstream promoters, while the U6 RNA gene from the yeast Saccharomyces cerevisiae (SNR6) has internal and downstream promoter elements that match the tRNA gene intragenic A- and B-block elements, respectively. Substitution of the SNR6 A or B block greatly diminished U6 RNA accumulation in vivo, and a subcellular extract competent for RNA polymerase III transcription generated nearly identical DNase I protectio
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Eschenlauer, J. B., M. W. Kaiser, V. L. Gerlach, and D. A. Brow. "Architecture of a yeast U6 RNA gene promoter." Molecular and Cellular Biology 13, no. 5 (1993): 3015–26. http://dx.doi.org/10.1128/mcb.13.5.3015-3026.1993.

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The promoters of vertebrate and yeast U6 small nuclear RNA genes are structurally dissimilar, although both are recognized by RNA polymerase III. Vertebrate U6 RNA genes have exclusively upstream promoters, while the U6 RNA gene from the yeast Saccharomyces cerevisiae (SNR6) has internal and downstream promoter elements that match the tRNA gene intragenic A- and B-block elements, respectively. Substitution of the SNR6 A or B block greatly diminished U6 RNA accumulation in vivo, and a subcellular extract competent for RNA polymerase III transcription generated nearly identical DNase I protectio
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Joazeiro, C. A., G. A. Kassavetis, and E. P. Geiduschek. "Identical components of yeast transcription factor IIIB are required and sufficient for transcription of TATA box-containing and TATA-less genes." Molecular and Cellular Biology 14, no. 4 (1994): 2798–808. http://dx.doi.org/10.1128/mcb.14.4.2798.

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Specific transcription by RNA polymerase III requires recognition of the promoter-bound transcription factor IIIB (TFIIIB), of which the TATA-binding protein (TBP) is a subunit. The recruitment of TFIIIB to TATA-less genes is mediated by protein-protein interactions with transcription factor IIIC (TFIIIC) bound to the box A and box B elements. Here we examine interactions involved in the recruitment of TFIIIB to the TATA element-containing yeast U6 small nuclear RNA gene SNR6. TFIIIC is not required for the formation of TFIIIB-SNR6 gene complexes with purified components. The same three compon
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Joazeiro, C. A., G. A. Kassavetis, and E. P. Geiduschek. "Identical components of yeast transcription factor IIIB are required and sufficient for transcription of TATA box-containing and TATA-less genes." Molecular and Cellular Biology 14, no. 4 (1994): 2798–808. http://dx.doi.org/10.1128/mcb.14.4.2798-2808.1994.

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Specific transcription by RNA polymerase III requires recognition of the promoter-bound transcription factor IIIB (TFIIIB), of which the TATA-binding protein (TBP) is a subunit. The recruitment of TFIIIB to TATA-less genes is mediated by protein-protein interactions with transcription factor IIIC (TFIIIC) bound to the box A and box B elements. Here we examine interactions involved in the recruitment of TFIIIB to the TATA element-containing yeast U6 small nuclear RNA gene SNR6. TFIIIC is not required for the formation of TFIIIB-SNR6 gene complexes with purified components. The same three compon
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Shivaswamy, Sushma, George A. Kassavetis, and Purnima Bhargava. "High-Level Activation of Transcription of the Yeast U6 snRNA Gene in Chromatin by the Basal RNA Polymerase III Transcription Factor TFIIIC." Molecular and Cellular Biology 24, no. 9 (2004): 3596–606. http://dx.doi.org/10.1128/mcb.24.9.3596-3606.2004.

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ABSTRACT Transcription of the U6 snRNA gene (SNR6) in Saccharomyces cerevisiae by RNA polymerase III (pol III) requires TFIIIC and its box A and B binding sites. In contrast, TFIIIC has little or no effect on SNR6 transcription with purified components in vitro due to direct recognition of the SNR6 TATA box by TFIIIB. When SNR6 was assembled into chromatin in vitro by use of the Drosophila melanogaster S-190 extract, transcription of these templates with highly purified yeast pol III, TFIIIC, and TFIIIB displayed a near-absolute requirement for TFIIIC but yielded a 5- to 15-fold-higher level o
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Gerlach, V. L., S. K. Whitehall, E. P. Geiduschek, and D. A. Brow. "TFIIIB placement on a yeast U6 RNA gene in vivo is directed primarily by TFIIIC rather than by sequence-specific DNA contacts." Molecular and Cellular Biology 15, no. 3 (1995): 1455–66. http://dx.doi.org/10.1128/mcb.15.3.1455.

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The Saccharomyces cerevisiae U6 RNA gene (SNR6), which is transcribed by RNA polymerase III, has an unusual combination of promoter elements: an upstream TATA box, an intragenic A block, and a downstream B block. In tRNA genes, the A and B blocks are binding sites for the transcription initiation factor TFIIIC, which positions TFIIIB a fixed distance upstream of the A block. However, in vitro transcription of SNR6 with purified components requires neither TFIIIC nor the A and B blocks, presumably because TFIIIB recognizes the upstream sequences directly. Here we demonstrate that TFIIIB placeme
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Martin, Michael P., Valerie L. Gerlach, and David A. Brow. "A Novel Upstream RNA Polymerase III Promoter Element Becomes Essential When the Chromatin Structure of the Yeast U6 RNA Gene Is Altered." Molecular and Cellular Biology 21, no. 19 (2001): 6429–39. http://dx.doi.org/10.1128/mcb.21.19.6429-6439.2001.

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ABSTRACT The Saccharomyces cerevisiae U6 RNA gene,SNR6, possesses upstream sequences that allow productive binding in vitro of the RNA polymerase III (Pol III) transcription initiation factor IIIB (TFIIIB) in the absence of TFIIIC or other assembly factors. TFIIIC-independent transcription ofSNR6 in vitro is highly sensitive to point mutations in a consensus TATA box at position −30. In contrast, the TATA box is dispensable for SNR6 transcription in vivo, apparently because TFIIIC bound to the intragenic A block and downstream B block can recruit TFIIIB via protein-protein interactions. A muta
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Kumar, A., G. A. Kassavetis, E. P. Geiduschek, M. Hambalko, and C. J. Brent. "Functional dissection of the B" component of RNA polymerase III transcription factor IIIB: a scaffolding protein with multiple roles in assembly and initiation of transcription." Molecular and Cellular Biology 17, no. 4 (1997): 1868–80. http://dx.doi.org/10.1128/mcb.17.4.1868.

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Transcription factor IIIB (TFIIIB), the central transcription factor of Saccharomyces cerevisiae RNA polymerase III, is composed of TATA-binding protein, the TFIIB-related protein Brf, and B". B", the last component to enter the TFIIIB-DNA complex, confers extremely tight DNA binding on TFIIIB. Terminally and internally deleted B" derivatives were tested for competence to form TFIIIB-DNA complexes by TFIIIC-dependent and -independent pathways on the SUP4 tRNA(Tyr) and U6 snRNA (SNR6) genes, respectively, and for transcription. Selected TFIIIB-TFIIIC-DNA complexes assembled with truncated B" we
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Arrebola, Rosalía, Nathalie Manaud, Sophie Rozenfeld та ін. "τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding". Molecular and Cellular Biology 18, № 1 (1998): 1–9. http://dx.doi.org/10.1128/mcb.18.1.1.

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ABSTRACT Transcription factor IIIC (TFIIIC) (or τ) is a large multisubunit and multifunctional factor required for transcription of all class III genes in Saccharomyces cerevisiae. It is responsible for promoter recognition and TFIIIB assembly. We report here the cloning and characterization of TFC6, an essential gene encoding the 91-kDa polypeptide, τ91, present in affinity-purified TFIIIC. τ91 has a predicted molecular mass of 74 kDa. It harbors a central cluster of His and Cys residues and has basic and acidic amino acid regions, but it shows no specific similarity to known proteins or pred
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Dissertations / Theses on the topic "Transcription du gène SNR6 (U6)"

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Moenne, Alejandra. "Étude du système de transcription de classe C chez la levure : analyse de mutants conditionnels de l'ARN polymérase C." Paris 11, 1988. http://www.theses.fr/1988PA112334.

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This thesis concerns the study of class C gene transcription in the yeast Saccharomyces cerevisiae by taking advantage of temperature sensitive mutants affected at the level of RNA polymerase C. The mutants rpc160-20, rpc160-31and rpc160-41 carry a point mutation in the major subunit (C160) of RNA polymerase C, and the mutant RPC53::H+ carries an integrative disruption of the gene coding for the subunit C53. In vivo, the mutants display an imbalance in the synthesis of the small RNAs transcribed by RNA polymerase C : the synthesis of tRNAs is greatly diminished compared to that of 5S RNA. The
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