Relevant bibliographies by topics / Transfection

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Transfection'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 July 2025

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Journal articles on the topic "Transfection"

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Cheow, Pheik-Sheen, Tiong Kit Tan, Adelene Ai-Lian Song, Khatijah Yusoff, and Suet Lin Chia. "An improved method for the rescue of recombinant Newcastle disease virus." BioTechniques 68, no. 2 (2020): 96–100. http://dx.doi.org/10.2144/btn-2019-0110.

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Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfec
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Zhang, Jianxiong, Yawei Hu, Xiaoqing Wang, Peng Liu, and Xiaofang Chen. "High-Throughput Platform for Efficient Chemical Transfection, Virus Packaging, and Transduction." Micromachines 10, no. 6 (2019): 387. http://dx.doi.org/10.3390/mi10060387.

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Intracellular gene delivery is normally required to study gene functions. A versatile platform able to perform both chemical transfection and viral transduction to achieve efficient gene modification in most cell types is needed. Here we demonstrated that high throughput chemical transfection, virus packaging, and transduction can be conducted efficiently on our previously developed superhydrophobic microwell array chip (SMAR-chip). A total of 169 chemical transfections were successfully performed on the chip in physically separated microwells through a few simple steps, contributing to the co
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Toth, Maria, Manuel Reithofer, Gregory Dutra, Patricia Pereira Aguilar, Astrid Dürauer, and Reingard Grabherr. "Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells." Viruses 16, no. 3 (2024): 426. http://dx.doi.org/10.3390/v16030426.

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(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or on laborious generation of stable cell lines. Alternatively, BacMam, a transduction process using the baculovirus, can be employed. (2) Six transfecting agents were compared to baculovirus transduction in terms of transient and stable protein expression characteristics of the model protein ACE2-eGFP using HEK293-6E, CHO-K1, and Vero cell lines. Furthermore, process optimization such as expression enhancement using sodium
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Gam, Jeremy J., Breanna DiAndreth, Ross D. Jones, Jin Huh, and Ron Weiss. "A ‘poly-transfection’ method for rapid, one-pot characterization and optimization of genetic systems." Nucleic Acids Research 47, no. 18 (2019): e106-e106. http://dx.doi.org/10.1093/nar/gkz623.

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Abstract Biological research is relying on increasingly complex genetic systems and circuits to perform sophisticated operations in living cells. Performing these operations often requires simultaneous delivery of many genes, and optimizing the stoichiometry of these genes can yield drastic improvements in performance. However, sufficiently sampling the large design space of gene expression stoichiometries in mammalian cells using current methods is cumbersome, complex, or expensive. We present a ‘poly-transfection’ method as a simple yet high-throughput alternative that enables comprehensive
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Gardiner, Donald L., Tina S. Skinner-Adams, Tobias Spielmann, and Katharine R. Trenholme. "Malaria transfection and transfection vectors." Trends in Parasitology 19, no. 9 (2003): 381–83. http://dx.doi.org/10.1016/s1471-4922(03)00187-9.

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Peng, Zhiqiang, Jianping Xiong, Hanzhi Dong, and Wuping Li. "Preparation of Polymer Nanocarrier Material and Its Application in B-Cell Lymphoma." Science of Advanced Materials 12, no. 10 (2020): 1524–34. http://dx.doi.org/10.1166/sam.2020.3877.

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The β-cyclodextrin (β-CD) is coupled with polyethyleneimine (PEI 600 Da) to produce a polymer nanocarrier material (PyD-W) with good biocompatibility and high transfection rate. First, the test was performed to study the influence of different factors on the transfection efficiency of PyD-W materials in terms of cell type and transfection system. Then the effect of adding wheat germ agglutinin on the material-cell membrane binding when transfecting cells by PyD-W materials was studied. The influence of temperature and cell phagocytosis inhibitors on the entire transfection process were taken i
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Yin, Jingwen, and Henry R. Henney Jr. "Stable transfection ofAcanthamoeba." Canadian Journal of Microbiology 43, no. 3 (1997): 239–44. http://dx.doi.org/10.1139/m97-033.

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The promoter activity of an Acanthamoeba polyubiquitin gene was analyzed in its homologous system. A modified calcium phosphate transfection method using a neomycin marker vector was developed to achieve highly efficient transfection of the Acanthamoeba polyubiquitin gene into Acanthamoeba cells. In this transfection procedure, the calcium phosphate – DNA complex was formed gradually in the medium during incubation with cells and precipitated on the cells. The crucial factors for obtaining efficient transfection were the pH (6.95) of the transfection buffer used for the calcium phosphate preci
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Nie, Leng, Li Zeng Gao, Xi Yun Yan, and Tai Hong Wang. "Functionalized Tetrapod-Like ZnO Nanostructrures for DNA Gene Delivery." Solid State Phenomena 121-123 (March 2007): 747–50. http://dx.doi.org/10.4028/www.scientific.net/ssp.121-123.747.

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Amino-modified tetrapod-like ZnO nanostructures were tried as novel carriers for mammalian cell transfections. The nanostructures consisted of four needle-shaped tetrahedrally arranged legs connected at the center. After silica coating and amino modification, ZnO nanostructures complexes bound plasmid DNA through electrostatic interactions in aqueous solution. When mixed with cells, DNA-nanostructures attached easily onto cell membranes and entered the cells for gene expressions. Due to high positive charge densities on surfaces and needle-shaped tetrahedral structures, functionalized ZnO used
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Chung, Namjin, Louis Locco, Kevin W. Huff, et al. "An Efficient and Fully Automated High-Throughput Transfection Method for Genome-Scale siRNA Screens." Journal of Biomolecular Screening 13, no. 2 (2008): 142–48. http://dx.doi.org/10.1177/1087057107312032.

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RNA interference (RNAi), combined with the availability of genome sequences, provides an unprecedented opportunity for the massive and parallel investigations of gene function. Small interfering RNA (siRNA) represents a popular and quick approach of RNAi for in vitro loss-of-function genetic screens. Efficient transfection of siRNA is critical for unambiguous interpretation of screen results and thus overall success of any siRNA screen. A high-throughput, lipid-based transfection method for siRNA was developed that can process eighty 384-well microplates in triplicate (for a total of 30,720 un
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Chong, Kevin Wai Yin, Alan Yiu-Wah Lee, Evelyn S. C. Koay, Sze Jee Seet, and Nam Sang Cheung. "pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin." Journal of Neuroscience Methods 158, no. 1 (2006): 56–63. http://dx.doi.org/10.1016/j.jneumeth.2006.05.017.

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Dissertations / Theses on the topic "Transfection"

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Ma, Nan. "Tailoring optical fibers for cell transfection." Thesis, University of St Andrews, 2012. http://hdl.handle.net/10023/3177.

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Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane. Of the different optical light sources that have been used, femtosecond laser based transfection has been one of the most effective methods for optical transfection which is generally implemented using a free-space bulk optical setup. Here in this thesis, a few novel fabrication methods are devised to obtain easy and inexpensive fabrication of microlensed optical fibers, which can be used to replace traditional optical setup and pe
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Finlay, Siân. "Modulation of macrophage phenotype using adenoviral transfection." Thesis, University of Aberdeen, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409252.

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The initial aim of this study was to examine the nature of the interaction between adenovirus and transfected macrophage, and to characterise the molecular mechanisms underlying macrophage response to adenoviral infection. Results showed that adenoviral transfection activated macrophages, promoting production of pro-inflammatory mediators and priming an enhanced response to other inflammatory stimuli.  Activation was dependent on the nuclear factor kappa B (NF<span style='font-family:Symbol'>kB) signalling pathway, which was activated within two hours of transfection, and could be prevented us
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Horefti, Ioulia-Christina. "Aeroporation : a new method for cell transfection." Thesis, University of Essex, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364549.

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Yingyongnarongkul, Boon-ek. "Transfection agents : from traditional to miniaturised screening." Thesis, University of Southampton, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289566.

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Tsampoula, Xanthi. "Femtosecond cellular transfection using novel laser beam geometries." Thesis, St Andrews, 2009. http://hdl.handle.net/10023/909.

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Mthunzi, Patience. "Optical sorting and photo-transfection of mammalian cells." Thesis, University of St Andrews, 2010. http://hdl.handle.net/10023/1254.

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Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic channels and the cytoplasmic delivery of both genetic and non-genetic matter of varying sizes into mammalian cells. In this thesis several new findings specifically in the optical cell sorting as well as in the photo-transfection study fields are presented. In my optical cell sorting and guiding investigations, a new technique for enhancing the
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Lemoine, Jérôme. "Transfection de l'épithélium respiratoire nasal normal de souris." Reims, 2005. http://www.theses.fr/2005REIMP205.

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La @mucoviscidose est une maladie génétique affectant principalement les poumons, qui est due à la mutation du gène CFTR. La protéine CFTR est un canal chlorique. Le transfert du gène CFTR sauvage dans l'épithélium bronchique peut être obtenu à l'aide de vecteurs de gènes non-viraux. Ces transferts n'ont pas permis, jusqu'à présent, de corriger complètement le transport de chlorure. Dans cette étude, nous avons démontré que de l'ADN nu dissout dans de l'eau déminéralisée transfecte les cellules épithéliales nasales plus efficacement que dans une solution isotonique ou hypertonique. Cette métho
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Le, Bolc'h Gwénaelle. "Synthèse de phosphonolipides pour la transfection non-virale." Brest, 1997. http://www.theses.fr/1997BRES2032.

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La therapie genique apparait, de plus en plus, comme une solution envisageable pour vaincre les maladies hereditaires comme la mucoviscidose par exemple. L'objectif de ce travail est la synthese de composes pouvant favoriser la transfection d'un gene correcteur a travers les membranes cellulaires. Le premier chapitre bibliographique fait le point sur la therapie genique et sur les differents vecteurs non-viraux deja connus. Les deux chapitres suivants sont consacres a la synthese de composes susceptibles d'etre utilises comme vecteurs non-viraux de transfection. Les premieres evaluations de la
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Lemoine, Jérôme Desoize Bernard. "Transfection de l'épithélium respiratoire nasal normal de souris." S.l. : S.n, 2005. http://www.univ-reims.fr/BU//exl-doc/GED00000194.pdf.

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DUGONNET, DURAND FREDERIQUE. "Differentes methodes de transfection appliquees en therapie genique." Strasbourg 1, 1994. http://www.theses.fr/1994STR15097.

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Books on the topic "Transfection"

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Bielke, Wolfgang, and Christoph Erbacher, eds. Nucleic Acid Transfection. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-16430-9.

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Christoph, Erbacher, and SpringerLink (Online service), eds. Nucleic Acid Transfection. Springer-Verlag Berlin Heidelberg, 2010.

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Ruth, Laura. Transfection and gene transfer: Technologies and markets. Kalorama Information, 2002.

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Yanan, Yue. How Free Cationic Polymer Chains Promote Gene Transfection. Springer International Publishing, 2013. http://dx.doi.org/10.1007/978-3-319-00336-8.

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Zarytova, V. F. Polyamine-containing DNA fragments. Nova Science Publishers, 2011.

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Zarytova, V. F. Polyamine-containing DNA fragments. Nova Science Publishers, 2011.

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Jassal, Ramesh Kumar. Remodelling recombinant glycoproteins made in CHO cells by transfection of glycosyltransferases. De Montfort University, 1999.

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Wong, Michael J. Transfection and expression of human factor I cDNA in mammalian cells. National Library of Canada = Bibliothèque nationale du Canada, 1993.

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Moyle, Katherine. La transfection de fibroblastes normaux par des ADN de papillomavirus humains. Université Laurentienne, 1992.

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Lasserre, Chantal. Hybridation cellulaire et transfection: Étude du maintien et de l'expression du matériel génétique introduit. A.N.R.T. Université Pierre Mendès France Grenoble 2, 1986.

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Book chapters on the topic "Transfection"

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Mehlhorn, Heinz. "Transfection." In Encyclopedia of Parasitology. Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-43978-4_4415.

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Mehlhorn, Heinz. "Transfection." In Encyclopedia of Parasitology. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-27769-6_4415-1.

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Feril, Loreto B. "Ultrasound-Mediated Gene Transfection." In Gene Therapy of Cancer. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-561-9_10.

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Fischer, Wiebke, Marcelo Calderón, and Rainer Haag. "Hyperbranched Polyamines for Transfection." In Topics in Current Chemistry. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/128_2010_64.

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Franklin, Roger, and Julian E. Sale. "Transient transfection of DT40." In Subcellular Biochemistry. Springer Netherlands, 2006. http://dx.doi.org/10.1007/978-1-4020-4896-8_29.

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Turrin, Cédric-Olivier, and Anne-Marie Caminade. "Dendrimers as Transfection Agents." In Dendrimers. John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9781119976530.ch17.

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González, Blanca. "Ceramics for Gene Transfection." In Bio-Ceramics with Clinical Applications. John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118406748.ch13.

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Amin, Karan Naresh, Ravichandran Jayasuriya, R. Senthilkumar, and K. M. Ramkumar. "Vector Transfection and Validation." In Advanced Mammalian Cell Culture Techniques. CRC Press, 2023. http://dx.doi.org/10.1201/9781003397755-30.

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Girard, Philippe, M. Derouazi, G. Baumgartner, M. Bourgeois, Martin Jordan, and Florian M. Wurm. "100 Liter Transient Transfection." In Animal Cell Technology: From Target to Market. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0369-8_10.

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Bøe, Sigurd Leinæs, and Eivind Hovig. "Light-Induced mRNA Transfection." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-260-5_6.

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Conference papers on the topic "Transfection"

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Ma, Yibo, Wenjing Huang, and Yoko Yamanishi. "Cell transfection efficiency improvement via applying stimulation based on the electrically induced microbubbles." In 2024 IEEE International Conference on Cyborg and Bionic Systems (CBS). IEEE, 2024. https://doi.org/10.1109/cbs61689.2024.10860468.

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Tsong, Tian Y., and Ting Dong Xie. "Mechanisms of cell transfection by electroporation: Field induced DNA uptake and transfection efficiency." In 1992 14th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1992. http://dx.doi.org/10.1109/iembs.1992.5760978.

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Palanker, D., T. Chalberg, A. Vankov, et al. "Plasma-mediated transfection of RPE." In Biomedical Optics 2006, edited by Fabrice Manns, Per G. Söderberg, and Arthur Ho. SPIE, 2006. http://dx.doi.org/10.1117/12.649624.

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Stevenson, David, Ben Agate, Lynn Paterson, et al. "Optical transfection of mammalian cells." In Photonics Europe, edited by Romualda Grzymala and Olivier Haeberle. SPIE, 2006. http://dx.doi.org/10.1117/12.662325.

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James, Molly B., and Todd D. Giorgio. "Nuclear-Associated Plasmid, But Not Cell-Associated Plasmid, is Correlated With Transgene Expression in Cultured Mammalian Cells." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2232.

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Abstract Intracellular plasmid is rapidly incorporated into the nucleus of HeLa cells following cationic lipoplex transfection. CV1 cells are less effective in translocating plasmid to the nucleus and also express less transgene than HeLa cells. Cultured HeLa and CV1 cells and corresponding isolated nuclei were analyzed after transfection of a Cy3 labeled pGreenLantern plasmid (Cy3-pGL). Flow cytometry was used to measure both plasmid delivery and transgene expression from the plasmid encoding a CMV promoter driven green fluorescent protein. During transfection, HeLa cells rapidly incorporated
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Breunig, Hans Georg, Ana Batista, Aisada König, and Karsten König. "Towards laser-assisted microfluidic-cell transfection." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, edited by Daniel L. Farkas, James F. Leary, and Attila Tarnok. SPIE, 2019. http://dx.doi.org/10.1117/12.2509442.

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Otsuka, Risa, Mitsuhiro Terakawa, Shunichi Sato, et al. "Laser-induced stress wave-assisted gene transfection: improved transfection efficiency with cationic liposome-modified plasmid DNA." In Biomedical Optics (BiOS) 2007, edited by Steven L. Jacques and William P. Roach. SPIE, 2007. http://dx.doi.org/10.1117/12.701734.

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Zhu, Qingfu, Ziyu Zhu, and Mei He. "3D Additive Manufacturing and Micro-Assembly for Transfection of 3D-Cultured Cells and Tissues." In ASME 2018 13th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/msec2018-6567.

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3D additive manufacturing, namely 3D printing, has been increasingly needed in the fabrication of biological materials and devices. Compared to traditional fabrication, direct 3D digital transformation simplifies the manufacturing process and enhances capability in geometric fabrication. In this paper, we demonstrated a rapid and low-cost 3D printing approach for “lego” assembly of micro-structured parts as an electro-transfection device. Electro-transfection is an essential equipment for engineering and regulating cell biological functions. Nevertheless, existing platforms are mainly employed
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Lin, Yu-Cheng, Chung-Min Jen, Ming-Yuan Huang, and Xi-Zhang Lin. "Flow-type electroporation chips for gene transfection." In Micromachining and Microfabrication, edited by Carlos H. Mastrangelo and Holger Becker. SPIE, 2000. http://dx.doi.org/10.1117/12.395660.

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Jingcai Ma, Xiaona Cao, Shumin Zhou, et al. "Phosphorylatable short peptide for facilitating DNA transfection." In 2009 International Conference on Future BioMedical Information Engineering (FBIE). IEEE, 2009. http://dx.doi.org/10.1109/fbie.2009.5405819.

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Reports on the topic "Transfection"

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Sawangmake, Chenphop. Development of genetic manipulation approach for in vitro production of islet-like cell cluster (ILCCs) or insulin-producing cells (IPCs) from canine bone marrow-derived mesenchymal stem cells (cBM-MSCs). Chulalongkorn University, 2018. https://doi.org/10.58837/chula.res.2018.88.

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In this study, trend of stem cell-based treatment for diabetes type 1 in veterinary practice has been preliminarily investigated. Production of pancreatic lineages by canine bone marrow-derived mesenchymal stem cells (cBM-MSCs) using genetic manipulation approach has been studied. Transfection efficiency of second-generation lentiviral vector on cBM-MSCs employing “pLenti CMV GFP Puro (658-5)” (Addgene plasmid #17448) was investigated and the results suggested the susceptibility of the cell to such transfection. Further study was performed to evaluate the efficiency of PDX1 transfection on cBM
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Finch, Allison, Andrew Schaefer, Ephrath Tesfaye, et al. A proposal to align release standards for transfection reagents. BioPhorum, 2024. http://dx.doi.org/10.46220/2023cgt015.

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McCormick, J. J. Malignant transformation of diploid human fibroblasts by transfection of oncogenes. Office of Scientific and Technical Information (OSTI), 1992. http://dx.doi.org/10.2172/6852538.

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Kenney, James J. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes,. Defense Technical Information Center, 1992. http://dx.doi.org/10.21236/ada253974.

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Kenny, James J. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes. Defense Technical Information Center, 1991. http://dx.doi.org/10.21236/ada243424.

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Kenny, James J. Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes. Defense Technical Information Center, 1992. http://dx.doi.org/10.21236/ada245750.

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Kennedy, C. H., K. D. Kenyon, and J. Tesfaigzi. Transfection of normal human bronchial epithelial cells with the bcl-2 oncogene. Office of Scientific and Technical Information (OSTI), 1995. http://dx.doi.org/10.2172/381812.

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Radu, Daniela Rodica. Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection. Office of Scientific and Technical Information (OSTI), 2004. http://dx.doi.org/10.2172/837277.

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McCormick, J., and V. Maher. Malignant transformation of diploid human fibroblasts by transfection of oncogenes: Progress report, July 1986--June 1989. Office of Scientific and Technical Information (OSTI), 1989. http://dx.doi.org/10.2172/6067022.

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DeMartini, James C., Abraham Yaniv, Jonathan O. Carlson, et al. Evaluation of Naked Proviral DNA as a Vaccine for Ovine Lentivirus Infection. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7570553.bard.

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Ovine lentivirus (OvLV) infection is widespread in sheep of the United States and Israel and is responsible for substantial economic losses. The primary goal of this project was to evaluate naked proviral DNA as a vaccine to induce protective immunity in sheep in endemic areas. Contrary to expectations, inoculation of sheep with proviral DNA derived from the full length OvLV molecular clone pkv72 did not result in detectable OvLV infection, but infectious virus was recovered from transfected ovine cells. Kv72 virus produced by these cells infected sheep and induced antibody responses, and was
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