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Dissertations / Theses on the topic 'Transfection'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 July 2025

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1

Ma, Nan. "Tailoring optical fibers for cell transfection." Thesis, University of St Andrews, 2012. http://hdl.handle.net/10023/3177.

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Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane. Of the different optical light sources that have been used, femtosecond laser based transfection has been one of the most effective methods for optical transfection which is generally implemented using a free-space bulk optical setup. Here in this thesis, a few novel fabrication methods are devised to obtain easy and inexpensive fabrication of microlensed optical fibers, which can be used to replace traditional optical setup and pe
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2

Finlay, Siân. "Modulation of macrophage phenotype using adenoviral transfection." Thesis, University of Aberdeen, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409252.

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The initial aim of this study was to examine the nature of the interaction between adenovirus and transfected macrophage, and to characterise the molecular mechanisms underlying macrophage response to adenoviral infection. Results showed that adenoviral transfection activated macrophages, promoting production of pro-inflammatory mediators and priming an enhanced response to other inflammatory stimuli.  Activation was dependent on the nuclear factor kappa B (NF<span style='font-family:Symbol'>kB) signalling pathway, which was activated within two hours of transfection, and could be prevented us
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3

Horefti, Ioulia-Christina. "Aeroporation : a new method for cell transfection." Thesis, University of Essex, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364549.

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4

Yingyongnarongkul, Boon-ek. "Transfection agents : from traditional to miniaturised screening." Thesis, University of Southampton, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289566.

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5

Tsampoula, Xanthi. "Femtosecond cellular transfection using novel laser beam geometries." Thesis, St Andrews, 2009. http://hdl.handle.net/10023/909.

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6

Mthunzi, Patience. "Optical sorting and photo-transfection of mammalian cells." Thesis, University of St Andrews, 2010. http://hdl.handle.net/10023/1254.

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Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic channels and the cytoplasmic delivery of both genetic and non-genetic matter of varying sizes into mammalian cells. In this thesis several new findings specifically in the optical cell sorting as well as in the photo-transfection study fields are presented. In my optical cell sorting and guiding investigations, a new technique for enhancing the
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7

Lemoine, Jérôme. "Transfection de l'épithélium respiratoire nasal normal de souris." Reims, 2005. http://www.theses.fr/2005REIMP205.

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La @mucoviscidose est une maladie génétique affectant principalement les poumons, qui est due à la mutation du gène CFTR. La protéine CFTR est un canal chlorique. Le transfert du gène CFTR sauvage dans l'épithélium bronchique peut être obtenu à l'aide de vecteurs de gènes non-viraux. Ces transferts n'ont pas permis, jusqu'à présent, de corriger complètement le transport de chlorure. Dans cette étude, nous avons démontré que de l'ADN nu dissout dans de l'eau déminéralisée transfecte les cellules épithéliales nasales plus efficacement que dans une solution isotonique ou hypertonique. Cette métho
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8

Le, Bolc'h Gwénaelle. "Synthèse de phosphonolipides pour la transfection non-virale." Brest, 1997. http://www.theses.fr/1997BRES2032.

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La therapie genique apparait, de plus en plus, comme une solution envisageable pour vaincre les maladies hereditaires comme la mucoviscidose par exemple. L'objectif de ce travail est la synthese de composes pouvant favoriser la transfection d'un gene correcteur a travers les membranes cellulaires. Le premier chapitre bibliographique fait le point sur la therapie genique et sur les differents vecteurs non-viraux deja connus. Les deux chapitres suivants sont consacres a la synthese de composes susceptibles d'etre utilises comme vecteurs non-viraux de transfection. Les premieres evaluations de la
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9

Lemoine, Jérôme Desoize Bernard. "Transfection de l'épithélium respiratoire nasal normal de souris." S.l. : S.n, 2005. http://www.univ-reims.fr/BU//exl-doc/GED00000194.pdf.

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10

DUGONNET, DURAND FREDERIQUE. "Differentes methodes de transfection appliquees en therapie genique." Strasbourg 1, 1994. http://www.theses.fr/1994STR15097.

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11

Hogge, Donna Eileen. "Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/27316.

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In most neoplasms malignant change occurs in a single cell which then proliferates. My purpose was to explore methods to study the cell that gives rise to hemopoietic cancer and to investigate the abnormalities at a molecular level. Cytogenetic analysis of cells from individual hemopoietic colonies revealed that monosomy 7 syndrome, a hematologic disorder of childhood, arises in a primitive cell capable of differentiating down both myeloid and erythroid pathways. Long-term bone marrow cultures (LTC) from patients with chronic myelogenous leukemia (CML) favor the growth of Philadelphia chromo
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12

Siu, King-sun, and 蕭景新. "Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43223874.

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13

Lai, Wing-fu, and 黎永富. "Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46588127.

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14

Siu, King-sun. "Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43223874.

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15

Greentree, David. "Gene transfection in satellite cell transplantation for myocardial repair." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0027/MQ50779.pdf.

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16

Flobak, Åsmund. "Investigation of Transfection Using Silica-coated Cupric Oxide Nanowires." Thesis, Norges Teknisk-Naturvitenskaplige Universitet, 2013. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-20683.

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One-dimensional high aspect ratio nanostructures grown from a copper foil are considered for intracellular delivery of bioactive molecules, such as drugs and genetic material. The cupric oxide (CuO) nanowires are coated with silicon oxides (SiOx), and embedded in a transparent polymer membrane consisting of the photoresist SU-8 and polydimethylsiloxane (PDMS).Nanowires are envisaged as one way two deliver bioactive molecules to many cells in parallel. It has previously been shown that both HeLa and AR42J cells grow and spread on this nanowire surface. The overarching goal of the current projec
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17

Chapman, M. R. "The potential of polyethylene imine derivatives as transfection reagents." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597479.

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This work aimed to address these issues by taking a novel approach to both the design and analysis of a library of transfection reagent derivatives. The library was produced by derivatisation of polyethylene imine (PEI), already a relatively efficient transfection reagent.  However, rather than viewing derivatisation as an all or nothing process, our libraries apply derivatisation in a stepwise fashion to completely explore the chemical space. By using combinations of three simple derivatisations (methylation, benzylation and dodecylation), a large range of properties are produced from limited
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18

Fischer, Wiebke [Verfasser]. "siRNA transfection with dendritic core-shell nanocarriers / Wiebke Fischer." Berlin : Freie Universität Berlin, 2012. http://d-nb.info/1026695244/34.

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19

Uduehi, Aimalohi Natasha. "Transfection of mammalian cell lines with polycationic/DNA complexes." Thesis, University of Bath, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338407.

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20

Lycett, Gareth John. "DNA transfection of an Ades aegypti mosquito cell line." Thesis, University of Liverpool, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314572.

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21

Rodamporn, Somphop. "Microfluidic systems for cell transfection using sonoporation and electroporation." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/159193/.

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Studies into sonoporation and electroporation have grown rapidly in biotechnology and medicine in recent years. This research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation system and electroporation system with certain biological cells. Ultrasonic standing waves, previously used for ultrasonic particle manipulation, have been used in the development of the sonoporation aspects of the system. MATLAB has also been used to analyse the required acoustic conditions within the ch
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22

Brown, Taylor Andrew. "In Vivo Silicon Lance Array Transfection of Plant Cells." BYU ScholarsArchive, 2020. https://scholarsarchive.byu.edu/etd/8398.

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Arrays of silicon lances were made using photolithographic and STS DRIE Bosch techniques. Arrays consist of a 10 mm square grid pattern of lances measuring 100 m tall and having a 3 mm diameter, each lance being spaced 30 mm apart. The tips of lances are pointed, allowing easier penetration through plant cell walls. A nanoinjector device was also made to accept the silicon lance arrays and perform nanoinjections. A nanoinjection consisted of 2 silicon lance arrays, with lances oriented towards each other, being moved into and out of a plant cotyledon placed between them. Prior to the nanoinjec
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23

Bresler, Brandon G. "Organic Blue TADF Chromophore Tag For Monitoring Transfection Studies." Wright State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright161038158466956.

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24

Churamani-Poulenc, Rebecca. "Assemblages supramoléculaires de cyclodextrines fonctionnalisées comme outil de transfection." Electronic Thesis or Diss., Sorbonne université, 2024. http://www.theses.fr/2024SORUS078.

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La thérapie génique est un domaine scientifique crucial qui doit être davantage développé, et la crise du Covid-19 n'a fait que confirmer cette nécessité. Les virus sont connus pour être des vecteurs de matériel génétique très efficaces. Parmi eux, le virus de la mosaïque du tabac (TMV), dont le manteau protéique peut s'auto-assembler en un co-assemblage coopératif avec de l'ARN, a inspiré notre groupe pour concevoir un virus artificiel à base de cyclodextrines (CDs). Notre groupe a synthétisé une CD cationique portant un pont ammonium et sur le côté duquel est fonctionnalisé un groupement ada
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25

Fiff, Fabian. "Transfection of baboon dendritic cells with plasmid DNA containing HIV-1C genes : effect of transfection methods on antigen processing and presentation to T lymphocytes." Thesis, Link to the online version, 2005. http://hdl.handle.net/10019/1022.

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26

Escoffre, Jean-Michel. "Mécanismes moléculaires et cellulaire de l'électrotransfert de plasmides in vitro." Toulouse 3, 2010. http://thesesups.ups-tlse.fr/1296/.

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Le transfert de plasmides au sein d'une cellule cible représente un outil clé dans l'étude de fonctions biologiques et dans le développement de nouvelles approches thérapeutiques. Cependant, le transfert de plasmides doit être réalisé avec un minimum d'effets secondaires au niveau de la cellule cible. La technique d'électroperméabilisation est une méthode physique fondée sur la modulation du potentiel électrique transmembranaire natif de la cellule par un champ électrique externe. Cependant, l'utilisation rationnelle de l'électroperméabilisation en pharmacologie et en médecine ne pourra se fai
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27

Balcells, García Laura. "Multi-faced study for the development of enhanced transfection systems." Doctoral thesis, Universitat Ramon Llull, 2020. http://hdl.handle.net/10803/669861.

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L'actual transferència de coneixements des de l'escala de laboratori d'investigació als productes farmacèutics i les teràpies humanes és limitada, entre altres raons, a causa de la percepció de les cèl·lules com caixes negres, en què hi ha entrades i sortides però es presta poca atenció als processos cel·lulars interns que regeixen els resultats generals. Aquesta tesi doctoral proposa un mètode per estudiar els mecanismes cel·lulars que intervenen en la internalització NPs i la transfecció de cèl·lules, que sovint passen desapercebuts. És a dir, mitjançant el desenvolupament d'una nova classe
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28

Cartwright, Joseph. "CHO cell genetic instability : from transfection to stable cell line." Thesis, University of Sheffield, 2016. http://etheses.whiterose.ac.uk/13986/.

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Chinese hamster ovary (CHO) cells are the predominant host cell type used in the production of recombinant therapeutic proteins. They are chosen as hosts, because of their ability to create, fold and modify proteins in a manner that makes them compatible with the human immune system. Moreover, CHO cells are tried and tested model organisms for bioprocess platforms, meaning regulatory body approval for new therapeutics is relatively easy to achieve. CHO cells are inherently genetically unstable, which can lead to a decline in productivity and poses a threat to product quality heterogeneity of s
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29

Aravindan, Latha. "Engineering polyethylenimine- Based gene delivery vectors with improved transfection efficiency." Thesis, University of Reading, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.533763.

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30

Hufnagel, Hansjörg. "Overcoming barriers in non-viral gene transfection by PEI-25." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608478.

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31

Shuang, Liang. "New Approach To DNA Transfection And Genetics In Schistosome Parasites." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1424201510.

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32

François, Marion. "Mécanismes de transfection par la polyethylenimine : influence de la PEGylation." Université Louis Pasteur (Strasbourg) (1971-2008), 2007. http://www.theses.fr/2007STR13252.

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Le concept de thérapie génique, défini par l'utilisation d'acides nucléiques en tant que médicament, a émergé il y a une trentaine d'années. C'est un concept très attractif, puisque contrairement aux traitements palliatifs, elle permettrait la guérison de pathologies génétiques héréditaires, d'affections acquises, de maladies dégénérescentes ou encore d'infections virales. La mise en oeuvre de la thérapie génique nécessite une vectorisation de l'ADN afin de transporter les acides nucléiques dans le noyau des cellules en utilisant des systèmes de délivrances (vecteurs) d'origine naturel ou synt
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33

Leonhardt, Carolin Anke [Verfasser], and Joachim [Akademischer Betreuer] Rädler. "On quantitative mRNA transfection / Carolin Anke Leonhardt ; Betreuer: Joachim Rädler." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/111514491X/34.

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34

Venkatesh, Savitha. "Chitosan-mediated transfection and the role of cell surface interactions." Scholarly Commons, 1997. https://scholarlycommons.pacific.edu/uop_etds/2320.

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Transfection is the process of introducing DNA into cells and expression of the gene contained in the DNA. The DNA itself could be a functional gene, part of a gene, or a gene with the regulatory and transcribed sequences intact. The ability to transfect mammalian cells is a powerful tool that can be used to study the function and control of mammalian genes. Transfection of DNA into eukaryotic cells can be done in several ways. One of the methods of introducing foreign DNA into mammalian cells is known as cationic liposome-mediated transfection. Preliminary studies involved the development of
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35

Leonhardt, Carolin Anke Verfasser], and Joachim [Akademischer Betreuer] [Rädler. "On quantitative mRNA transfection / Carolin Anke Leonhardt ; Betreuer: Joachim Rädler." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/111514491X/34.

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36

Sabraoui, Abbas. "Régulation de la cavitation acoustique appliquée à la transfection cellulaire." Thesis, Lyon 1, 2012. http://www.theses.fr/2012LYO10007.

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Le travail présenté ici porte sur l’étude et le contrôle de la cavitation acoustique dans le but de développer un système de sonoporation efficace pour les cellules en suspension et les cellules adhérentes. Le manuscrit est composé de trois chapitres. Tout d’abord, une revue de la littérature sur les différentes techniques physiques utilisées en transfection cellulaire, et plus particulièrement la sonoporation. Il a été démontré que le principal mécanisme de la sonoporation est étroitement lié au phénomène de cavitation acoustique. Un contrôle de ce phénomène aléatoire apparaît alors intéressa
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37

Sabraoui, Abbas. "Régulation de la cavitation acoustique appliquée à la transfection cellulaire." Electronic Thesis or Diss., Lyon 1, 2012. http://www.theses.fr/2012LYO10007.

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Le travail présenté ici porte sur l’étude et le contrôle de la cavitation acoustique dans le but de développer un système de sonoporation efficace pour les cellules en suspension et les cellules adhérentes. Le manuscrit est composé de trois chapitres. Tout d’abord, une revue de la littérature sur les différentes techniques physiques utilisées en transfection cellulaire, et plus particulièrement la sonoporation. Il a été démontré que le principal mécanisme de la sonoporation est étroitement lié au phénomène de cavitation acoustique. Un contrôle de ce phénomène aléatoire apparaît alors intéressa
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38

Dréan, Mathilde. "Controlled synthesis of polyvinylamine-based (co)polymers for gene transfection." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066444.

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Le transfert de gènes consiste en l’introduction d’acides nucléiques au sein de cellules afin de modifier leur activité dans un but essentiellement thérapeutique. Pour préserver le matériel génétique de toute dégradation, il faut recourir à des vecteurs. Parmi ceux-ci, les polymères cationiques sont très prometteurs, en particulier, la polyéthylènimine, considérée comme le vecteur non-viral de référence. Néanmoins, il présente une cytotoxicité élevée. Ainsi, de nombreuses recherches ont pour but d’identifier et de développer de nouveaux polymères combinant efficacité de transfection et haute v
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39

Dréan, Mathilde. "Controlled synthesis of polyvinylamine-based (co)polymers for gene transfection." Electronic Thesis or Diss., Paris 6, 2016. http://www.theses.fr/2016PA066444.

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Le transfert de gènes consiste en l’introduction d’acides nucléiques au sein de cellules afin de modifier leur activité dans un but essentiellement thérapeutique. Pour préserver le matériel génétique de toute dégradation, il faut recourir à des vecteurs. Parmi ceux-ci, les polymères cationiques sont très prometteurs, en particulier, la polyéthylènimine, considérée comme le vecteur non-viral de référence. Néanmoins, il présente une cytotoxicité élevée. Ainsi, de nombreuses recherches ont pour but d’identifier et de développer de nouveaux polymères combinant efficacité de transfection et haute v
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40

Wang, Weiguang. "The study of the oncogenic effect of PAC3, PAX3-FKHR and IGF-II genes in rhabdomyosarcoma and medulloblastoma." Thesis, Manchester Metropolitan University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243719.

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41

Denoyelle, Séverine. "Synthèses et études physico-chimiques de bolaformes hémifluocarbonés asymétriques : applications à la transfection de gènes." Avignon, 2005. http://www.theses.fr/2005AVIG0224.

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Les travaux de recherche portent sur l'élaboration d'une nouvelle famille de composés bolaformes cationiques hémifluorocarbonés asymétriques et l'évaluation de leur aptitude à transfecter l'ADN. Les études physicochimiques réalisées par les techniques de diffusion de la lumière quasi élastique, de zêtamétrie, de microscopie électronique à transmission et d'électrophorèse sur gel d'agarose, ainsi que les études biologiques, ont permis de mieux comprendre la relation entre l'organisation supramoléculaire de ces bolaformes dans l'eau et leur capacité à complexer l'ADN et transfecter les cellules.
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42

Hlawaty, Hanna. "Effets de siRNA anti MMP-2 et d'inhibiteurs de récepteurs aux leucotriènes sur l'hyperplasie intimale post-angioplastie chez le lapin hypercholestérolémique." Paris 13, 2007. http://www.theses.fr/2007PA132024.

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Le premier chapitre porte sur une stratégie de thérapie génique basée sur le siRNA L’application de siRNA en présence des lipides est une solution prometteuse aux problèmes posés par les méthodes virales. Cependant il est nécessaire d’augmenter l’efficacité de transfection dans les cellules musculaires lisses (CML) in vivo. Dans les artères saines les CML sécrètent la métalloprotéase 2 (MMP-2) de façon constitutive. L’angioplastie par ballonnet provoque l’augmentation de la MMP-2, la migration des CML et l’hyperplasie intimale. Le but de cette partie de thèse était d’appliquer le siRNA contre
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43

Goula, Tamafouo Daniel G. "Contribution à l'optimisation du transfert de gènes in vivo : analyse des facteurs pouvant influencer la transfection par des complexes ADN/PEI ainsi que la stabilité de l'expression des transgènes." Paris, Muséum national d'histoire naturelle, 1999. http://www.theses.fr/1999MNHN0021.

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Pour pouvoir être applicables à la thérapie, les vecteurs de transfert de gènes doivent d'abord prouver leur efficacité et leur innocuité in vivo. Le polyethylenimine (PEI), polymère cationique ayant une grande capacité de protonation, présente de nombreuses caractéristiques qui en font un bon candidat vecteur pour la transfection somatique in vivo. En effet, son potentiel a été démontré dans de nombreux travaux effectues essentiellement in vitro (voir section ii. 5. 4. C de l'introduction). Nous avons choisi l'injection intracérébrale et l'injection intravasculaire pour analyser la capacité d
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44

PRATAMA, MUHAMMAD YOGI. "TRANSLATIONAL STUDIES ON MICRORNA IN HEPATOCELLULAR CARCINOMA: FROM PREDICTIVE AND PROGNOSTIC BIOMARKERS TO MOLECULAR EFFECTORS." Doctoral thesis, Università degli Studi di Trieste, 2020. http://hdl.handle.net/11368/2973744.

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Introduction Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, represents the seventh most frequent cancer and the fourth leading cause of cancer-related death worldwide. Due to unspecific sign and symptoms along with the inefficacy of current non-invasive diagnostic tools for detecting early stages HCC, majority of patients are diagnosed at advanced stages that is no longer eligible for curative treatments. Moreover, the long-term outcome of treatments remain unsatisfactory. To overcome these problems, a better diagnostic and predictive biomarker might be one of
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45

Torres, Maria Leilani. "Optical transfection and injection techniques applied to mammalian and embryonic cells." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/2547.

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The delivery of biomolecules into living cells is an important methodology in cell and molecular biology. Optical methods using lasers are attractive tools for such application. However, the interaction of the laser with the cell depends on the laser type and the parameters used. Hence, in this thesis, optical transfection and injection of both mammalian and embryonic cells is demonstrated using a variety of laser sources. Furthermore, some key issues are addressed by demonstrating alternative configurations of optoinjection and transfection systems to develop a robust, user-friendly device wi
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46

Wang, T. "Chitosan nanoparticles by ionic coacervation for protein release and gene transfection." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.487466.

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The Ph.D. dissertation focuses on providing in depth understanding and scientific Iaiowledge usable to realize the potential of chitosan nanoparticles as protein release vehicles and non-viral gene carriers for phannaceutical applications. The preparation ofthe nanoparticles was based on the ability of chitosan to undergo a liquid-gel transition due to ionic interaction with polyanion, such as tripolyphosphate (TPP..). The chitosan-TPP nanoparticles had a particle size range 100-250 nm, positive zeta potential around +28-+52 mV ·and exhibited a high positive surface charge across a wide pH
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47

Pan, Fang. "Self-assembly of amphiphilic peptides and their potential in gene transfection." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.496700.

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The surfactant-like peptides are amphiphillic molecules containing polar or charged amino acids moieties as hydrophilic heads and non-polar ammo acids as hydrophobic tails, their surfactant-like amphiphilicity is attractive to a wide range of biotechnological applications including regenerative medicine and drug delivery. Short and designed peptides are easy to synthesise. They are becoming increasingly popular for self-assembly studies as model systems. It is of bith fundamental and practical significance to explore how these designed peptide molecules aggregate in solution and adsorb at the
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48

Venter, Chrizelle. "Chitosan and quaternised chitosan polymers as gene transfection agents / Chrizelle Venter." Thesis, North-West University, 2005. http://hdl.handle.net/10394/1015.

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Several approaches have been employed for directing the intracellular trafficking of DNA to the nucleus. Cationic polymers have been used to condense and deliver DNA and a few specific examples using chitosan as cationic polymer have been described. The concerted efforts in gene therapy to date have provided fruitful achievements toward a new era of curing human diseases. A number of obstacles, however, still must be surmounted for successful clinical applications. Therefore, chitosan-plasmid and quaternised chitosan-plasmid complexes (polyplexes) were investigated for their ability to transfe
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49

Jassal, Ramesh Kumar. "Remodelling recombinant glycoproteins made in CHO cells by transfection of glycosyltranferases." Thesis, De Montfort University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391648.

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50

Angel, Matthew. "Extended transient transfection by repeated delivery of in vitro-transcribed RNA." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/47895.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2008.<br>Includes bibliographical references (p. 53-56).<br>An experimental study was performed to evaluate a novel method of controlling protein expression by repeatedly transfecting cells with in vitro-transcribed RNA. Transcripts encoding six factors, each known to play an important role in cell-type specification and maintenance, were designed and synthesized. Aspects of the design were optimized, and the intracellular stability and translation efficiency of the ivT RNA were quantifi
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