Academic literature on the topic 'Transformation, overexpression'

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Journal articles on the topic "Transformation, overexpression"

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Ghiselli, Giancarlo, and Renato V. Iozzo. "Overexpression of Bamacan/SMC3 Causes Transformation." Journal of Biological Chemistry 275, no. 27 (2000): 20235–38. http://dx.doi.org/10.1074/jbc.c000213200.

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Yan, Xiaolin, Marie-France Lebel-Beaucage, Samuel Tremblay, Line Cantin, Gary S. Shaw, and Elodie Boisselier. "Optimized transformation, overexpression and purification of S100A10." BioTechniques 67, no. 5 (2019): 246–48. http://dx.doi.org/10.2144/btn-2019-0081.

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As a member of the S100 protein family, S100A10 has already been purified. However, its purity, or even yield, have often not been reported in the literature. To facilitate future biophysical experiments with S100A10, we aimed to obtain it at a purity of at least 95% in a reasonably large amount. Here, we report optimized conditions for the transformation, overexpression and purification of the protein. We obtained a purity of 97% and performed stability studies by circular dichroism. Our data confirmed that the S100A10 obtained is suitable for experiments to be performed at room temperature u
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Knoops, Laurent, Yohan Royer, Stefan Constantinescu, and Jean-Christophe Renauld. "Overexpression of Jak Kinases Promotes In Vitro Transformation." Blood 104, no. 11 (2004): 4325. http://dx.doi.org/10.1182/blood.v104.11.4325.4325.

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Abstract The Jak-STAT pathway is responsible for signal transduction by a large number of cytokine receptors. While this pathway is normally tightly regulated, constitutive STAT3 and STAT5 activation is frequent in hematologic malignancies and contributes to oncogenesis. We took advantage of the IL-3-dependent pro-B Ba/F3 cell line to analyze the mechanisms of constitutive STAT activation in vitro. Ba/F3 cells transfected with a mutated hIL-9R (Ba/F3 Phe116) poorly respond to IL-9. However, after selection with this cytokine, we obtained cell lines that proliferated well in IL-9. After cytokin
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Knoops, L., T. Hornakova, Y. Royer, S. N. Constantinescu, and J.-C. Renauld. "JAK kinases overexpression promotes in vitro cell transformation." Oncogene 27, no. 11 (2007): 1511–19. http://dx.doi.org/10.1038/sj.onc.1210800.

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Iwakawa, Hidekazu, Benjamin C. Carter, Brett C. Bishop, Joe Ogas, and Stanton B. Gelvin. "Perturbation of H3K27me3-Associated Epigenetic Processes Increases Agrobacterium-Mediated Transformation." Molecular Plant-Microbe Interactions® 30, no. 1 (2017): 35–44. http://dx.doi.org/10.1094/mpmi-12-16-0250-r.

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Agrobacterium-mediated transformation is a core technology for basic plant science and agricultural biotechnology. Improving transformation frequency is a major goal for plant transgenesis. We previously showed that T-DNA insertions in some histone genes decreased transformation susceptibility, whereas overexpression of several Arabidopsis H2A and H4 isoforms increased transformation. Overexpression of several histone H2B and H3 isoforms had little effect on transformation frequency. However, overexpression of histone H3-11 (HTR11) enhanced transformation. HTR11 is a unique H3 variant that lac
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Moiola, Cristian, Paola De Luca, Kevin Gardner, Elba Vazquez, and Adriana De Siervi. "Cyclin T1 overexpression induces malignant transformation and tumor growth." Cell Cycle 9, no. 15 (2010): 3191–98. http://dx.doi.org/10.4161/cc.9.15.12526.

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Ahlemann, Martin, Reinhard Zeidler, Stephan Lang, Brigitte Mack, Markus Münz, and Olivier Gires. "Carcinoma-associated eIF3i overexpression facilitates mTOR-dependent growth transformation." Molecular Carcinogenesis 45, no. 12 (2006): 957–67. http://dx.doi.org/10.1002/mc.20269.

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Nagase, Toshihiko, Sumio Kawata, Hiromu Nakajima, et al. "Effect of farnesyltransferase overexpression on cell growth and transformation." International Journal of Cancer 80, no. 1 (1999): 126–33. http://dx.doi.org/10.1002/(sici)1097-0215(19990105)80:1<126::aid-ijc23>3.0.co;2-u.

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Grammatopoulos, G. A., E. Bell, L. Toole, A. Lumsden, and A. S. Tucker. "Homeotic transformation of branchial arch identity after Hoxa2 overexpression." Development 127, no. 24 (2000): 5355–65. http://dx.doi.org/10.1242/dev.127.24.5355.

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Overexpression of Hoxa2 in the chick first branchial arch leads to a transformation of first arch cartilages, such as Meckel's and the quadrate, into second arch elements, such as the tongue skeleton. These duplicated elements are fused to the original in a similar manner to that seen in the Hoxa2 knockout, where the reverse transformation of second to first arch morphology is observed. This confirms the role of Hoxa2 as a selector gene specifying second arch fate. When first arch neural crest alone is targeted, first arch elements are lost, but the Hoxa2-expressing crest is unable to develop
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Resar, Linda, Surajit Dhara, Takita Felder Sumter, et al. "STAT3: A Direct HMGA1 Gene Target Important in Lymphoid Malignancy." Blood 108, no. 11 (2006): 2222. http://dx.doi.org/10.1182/blood.v108.11.2222.2222.

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Abstract While the oncogenic properties of the high mobility group A1 (HMGA1) gene are well established, the molecular pathways that mediate transformation by HMGA1 have not been clearly defined. HMGA1 is widely overexpressed in hematologic malignancies and other human cancers. Moreover, its overexpression portends a poor prognosis in some tumors. The HMGA1 gene encodes the HMGA1a and -A1b chromatin binding proteins. We previously showed that overexpression of HMGA1a or -A1b confers a transformed phenotype in cultured, human lymphoid cells. More recently, we developed transgenic mice overexpre
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Dissertations / Theses on the topic "Transformation, overexpression"

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Devereaux, Alissa Corrine. "Transformation and overexpression of a MnSOD gene in perennial ryegrass (Lolium perenne L.)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ61891.pdf.

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Liu, Suling. "An estrogenically regulated potential tumor suppressor gene, protein tyrosine phosphatase γ (PTPγ), in human breast". The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1063374869.

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Wijesekara, Kolitha Bandara Verfasser], Heiko [Akademischer Betreuer] [Becker, Petr [Akademischer Betreuer] Karlovsky, and Brigitte [Akademischer Betreuer] Maass. "Development of a haploid transformation system and overexpression of Phytochrome B gene in Brassica napus L. / Kolitha Bandara Wijesekara. Gutachter: Heiko C. Becker ; Petr Karlovsky ; Brigitte Maass. Betreuer: Heiko C. Becker." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2007. http://d-nb.info/1044186674/34.

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Huang, Ching-Chung, and 黃建中. "Overexpression of p97Eps8 is required for v-Src-mediated transformation." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/78150825547595457261.

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碩士<br>國立成功大學<br>藥理學研究所<br>92<br>Eps8 (EGF receptor pathway substrate NO.8) is a common substrate of various receptor tyrosine kinases and cytoplasmic tyrosine kinase Src. It exists in two isoforms, p97Eps8 and p68Eps8 in many cell lines. Our previous studies have indicated that C3H10T1/2 fibroblast overexpressing p97Eps8 not only exhibits the ability of focus formation in cell culture but also promotes the tumor formation in mice as compared to the control cells. Since both tyrosyl phosphorylation and protein expression of Eps8 are elevated in v-Src transformed cell IV5, we wonder whether Ep
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Annon, Ali Hani Hamza. "Overexpression of Tobacco Osmotin Protein in Carrot (Daucus carota L.) to Enhance Drought Tolerance." Thesis, 2012. http://hdl.handle.net/1969.1/148151.

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Lack of water is one of the most significant issues that already threaten world agriculture as many countries are unable to meet the demand for water to grow the crops. To make matters worse, the water availability is expected to fall by half by 2050, thus severely restricting agriculture production. Genetic engineering of crops to enhance their tolerance to such unfavorable environment represents one of the few approaches that can help us address this problem. Osmotin and osmotin-like proteins are stress proteins, belonging to the plant PR-5 group of proteins, which induced in response to va
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Chen, Hsing-Ta, and 陳幸達. "Improving Genetic Stability by Utilizing Bacteria with Linear Transformation during Recombinant Protein Overexpression." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/ksdm78.

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碩士<br>國立臺北科技大學<br>化學工程所<br>93<br>In this research, we use linear transformation that takes advantage of the homologous sequence between two ends of linear DNA and the host cell E.coli, to transfer the target genes directly into the chromosome of the strain. Because this method enables expressing recombinant proteins continuously without plasmid, the problem of plasmid instability is solved. In practice, we use ZSC114, a multi-auxotrophic E.coli strain that cannot metabolize glucose, mannose and lactose, as our host cell. First, we unite each of the three metabolism-related genes with lacZ
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Lin, Chih-Ta, and 林志達. "The roles of HSP70 family members (HSPA14/HSPA4) in NBS1-overexpression mediated transformation and metastasis." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/40368025227667797389.

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碩士<br>國立陽明大學<br>生化暨分子生物研究所<br>95<br>NBS1, forming a complex with RAD50 and MRE11, is involved in repairing double-strand breaks in DNA. In our previous studies, overexpression of NBS1 contributes to transformation through the activation of phosphatidylinositol 3-kinase (PI3K)/Akt, and induces the epithelial-mesenchymal transition (EMT) through the upregulation of Snail1/MMP2 expression. Understanding the role of NBS1 in cell transformation and metastasis, the molecular mechanism of NBS1 is needed to be delineated. Heat shock protein 70 (HSP70) chaperones play an important role in binding to no
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Wijesekara, Kolitha Bandara. "Development of a haploid transformation system and overexpression of Phytochrome B gene in Brassica napus L." Doctoral thesis, 2007. http://hdl.handle.net/11858/00-1735-0000-000D-F22A-E.

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Books on the topic "Transformation, overexpression"

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Dyer, Paul S., Carol A. Munro, and Rosie E. Bradshaw. Fungal genetics. Edited by Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum, and Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0005.

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Fungi have been long used as model organisms to investigate genetic and cellular processes. An overview is provided of how fungi function at a genetic level, including ploidy, gene structure, and gene flow by sexual and asexual processes. The tools used to study fungal genetics are then described, such techniques having widespread applications in medical mycology research. Classical genetic analysis includes the use of gene mapping by sexual crossing and tetrad analysis, and forward genetic experimentation based on mutagenesis, for which various mutant screening approaches are described. Molec
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Book chapters on the topic "Transformation, overexpression"

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Rigó, Gábor, Csaba Papdi, and László Szabados. "Transformation Using Controlled cDNA Overexpression System." In Plant Salt Tolerance. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-986-0_19.

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Wang, Min, Runrun Sun, Qinglian Wang, and Baohong Zhang. "Overexpression of miRNA in Cotton via Agrobacterium-Mediated Transformation." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8952-2_19.

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Mann, David G. J., Peter R. LaFayette, Laura L. Abercrombie, Wayne A. Parrott, and C. Neal Stewart. "pANIC: A Versatile Set of Gateway-Compatible Vectors for Gene Overexpression and RNAi-Mediated down-Regulation in Monocots." In Plant Transformation Technologies. Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9780470958988.ch11.

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Zhang, Baohong, Min Wang, Xin Zhang, Chengqi Li, and Qinglian Wang. "Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-212-4_16.

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Matres, Jerlie Mhay, Erwin Arcillas, Maria Florida Cueto-Reaño, Ruby Sallan-Gonzales, Kurniawan R. Trijatmiko, and Inez Slamet-Loedin. "Biofortification of Rice Grains for Increased Iron Content." In Rice Improvement. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66530-2_14.

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AbstractDietary iron (Fe) deficiency affects 14% of the world population with significant health impacts. Biofortification is the process of increasing the density of vitamins and minerals in a crop, through conventional breeding, biotechnology approaches, or agronomic practices. This process has recently been shown to successfully alleviate micronutrient deficiency for populations with limited access to diverse diets in several countries (https://www.harvestplus.org/). The Fe breeding target in the HarvestPlus program was set based on average rice consumption to fulfil 30% of the Estimated Average Requirement of Fe in women and children. In this review, we present the reported transgenic approaches to increase grain Fe. Insertion of a single or multiple genes encoding iron storage protein, metal transporter, or enzyme involved in the biosynthesis of metal chelator in the rice genome was shown to be a viable approach to significantly increase grain-Fe density. The most successful approach to reach the Fe breeding target was by overexpression of multiple genes. Despite this success, a significant effort of 8–10 years needs to be dedicated from the proof of concept to varietal release. This includes large-scale plant transformation, event selection, collection of data for premarket safety assurance, securing biosafety permits for consumption and propagation, and collection of data for variety registration.
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Sinha, Sujata. "Trends and Challenges in Enzymatic Bioengineering of Natural Products to Industrially Valuable Products." In Research Advancements in Pharmaceutical, Nutritional, and Industrial Enzymology. IGI Global, 2018. http://dx.doi.org/10.4018/978-1-5225-5237-6.ch016.

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Bioengineering of natural products to useful products are trending rapidly. These products are cost effective and eco-friendly and fitting into consumer demands of natural and organic. Wastes from industries, agriculture, fishery, dairy, etc. are being investigated for transformation to useful biomolecules for other industries like cosmetics, food supplements/preservation, dairy, etc. Biocatalytic transformation looks promising in the present scenario, but needs intensive research looking for novel enzymes/process and their optimization. Immobilization and scale up is also required for taking this process up to industrial level. Process improvement and downstream processing research for product purification is going on. Some of the areas which look promising are metagenomic screening of novel biocatalysts, gene cloning for overexpression and purification, etc. Bioreactor designing for scale up and simultaneous production and purification of desirable products are also being emphasised. Exploring biological activities after enzymatic reaction is one of the main areas of research nowadays.
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Xia, Wei. "Genes Involved in Lipid Metabolism in Coconut." In Innovation in the Food Sector Through the Valorization of Food and Agro-Food By-Products. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.90998.

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Coconut palm (Cocos nucifera L) is an economically important monocot plant grown in tropical and subtropical regions. Coconut oil is stored in a solid endosperm and has 47.48–50.5% fatty acid component as lauric acid (C12:0). Present research showed that acyl-acyl carrier protein thioesterases (FatA/B) and lysophosphatidic acid acyltransferase (LAAPT) are key enzymes determining medium-chain fatty acid accumulation in coconut oil. Among five CnFatB genes, CnFatB3 expressed specifically in endosperm and in vitro experiment showed that this gene made mainly lauric acid (C12:0) and tetradecenoic acid (C14:1). Overexpression of CnFatB3 in Arabidopsis increased the amounts of C12:0 and C14:0 in transgenic plant. CnLPAAT gene that is expressed specifically in coconut endosperm showed a preference for using acyl-CoAs containing C10:0, C12:0, and C14:0 acyl groups as acyl-donor substrates. Coconut and oil palm are closely related species with approximately 50% lauric acid (C12:0) in their endosperm. The two species have a close evolutionary relationship between predominant gene isoforms and high conservation of gene expression bias in the lipid metabolism pathways. Moreover, since no stable transformation system has been constructed in coconut palm, gene function validations have been done in vitro, or genes transformed into a heterologous system.
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"Flachmann (1997) studied the PS II antennae composition under varying light conditions in tobacc o plants transformed with antisense echnique. An increase of P S II antenna size was observed under low irradiance and also higher LHC II content. The results also suggested that LHC II biogenesis is perhaps not controlled by transcription. The foregone account of different studies using transgenics have inmmensely helped by adding new dimension in our understanding of the structure and function of the photosystem core complexes and of the antennae systems related to both PS II and PS I. A fairly larg e number of studies have also been directed using transgenic technology to understand the process of photoinhibition. Tyystjarvi et al., (1999b) have made a study of photoinhibition of PS II in tobacco an d poplar plants. The tobacco cultivars were expressed with bacterial gov gene in the cytosol and Fe SOD gene from Arabidopsis thaliana rather in the chloroplast. The transformations were affected as an overexpression of glutathione reductase in tobacco and superoxide dismutase in poplar. This transformation resulted in the activities of glutathione reductase in tobacco leaves and superoxide dismutase in poplars were five to eight times higher than in the untransformed plants. The experiments of the authors (Tyystjarvi et al., (1999b) with the transformed plants have led to some important clues regarding the identity of Active Oxygen Species and the mechanisms. There was a lack of protection by overproduction of SOD in the stroma, suggesting that superoxide is not accessible to dismutation by the stromal enzymes. Protection by glutathione reductase suggested that a soluble reductant has a limited chance to trap the species before it reacts with PS II RC. It was concluded (Tyystjarvi et al., 1999b) that much further work is required to understand the molecular mechanism of loss of PS II activity. H.Y.Yamamoto and his scholars have made several studies manipulating the levels of the enzymes of the xanthophyll cycle through transgenic techniques. Verhoeven et al., (2001) have investigated the effect of suppression of Z in tobacco plants with an antisense construct of VDE in growth chambers. Under short-term (2 or 3h) high light treatment, antisense plants had a greater reduction in Fv/Fm ratio relative to wild type, which implied a greater susceptibity to photoinhibition. In the long-term highlight stress experiment, the antisense plants had significant reduction in Fv/Fm. The authors concluded that XC-dependent energy dissipiation is critical for photoprotection in tobacco under excess light in the long term." In Photosynthesis. CRC Press, 2004. http://dx.doi.org/10.1201/9781482294446-20.

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Conference papers on the topic "Transformation, overexpression"

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Zhang, Dang-Quan, Zhen-Jun Gu, Shun-Yang Deng, Shao-Gang Fan, and Quan-Dong Zhu. "Construction of a New Universal Plant Overexpression Vector for cDNA Transformation." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5517341.

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Della-Franca, Austin, Daniel R. Catchpoole, Robert K. Bright, Anna deFazio, and Jennifer A. Byrne. "Abstract 2338: Molecular mechanisms underlying cellular transformation by Tumor protein D52 overexpression." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-2338.

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Wang, Xiran, Leiyu Jiang, and Haoru Tang. "Cloning and Construction of Overexpression Vector for FaUVR8 Gene Transformation with Strawberry." In 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017). Atlantis Press, 2018. http://dx.doi.org/10.2991/ifeesm-17.2018.350.

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Zhu, Yusha, Hong Sun, and Max Costa. "Abstract 1480: Overexpression of histone H3.1 induced cell malignant transformation and mutation of H3.1 C96/110A further induced chromatin instability." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-1480.

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Zimberlin, Maria Noel, Natalia Rubinstein, Omar A. Coso, and Edith C. Kordon. "Abstract B13: R‐spondin3 overexpression induces transformation and deregulates expression of genes controlling cell proliferation and apoptosis in NIH3T3 cells." In Abstracts: First AACR International Conference on Frontiers in Basic Cancer Research--Oct 8–11, 2009; Boston MA. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.fbcr09-b13.

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Das, Jayanta, and Deodutta Roy. "Abstract 803: Overexpression of NRF1 leads to the generation of cancer stem-like cells and resistance to anoikis _ pathways to anchorage-independent growth during estrogen-induced malignant transformation." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-803.

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Reports on the topic "Transformation, overexpression"

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Carbone, Christopher. MDM2 Overexpression Cooperates With Mutant CDK4 in Mammary Cell Transformation and Tumorigenesis. Defense Technical Information Center, 2004. http://dx.doi.org/10.21236/ada429494.

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