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1
Abraham, Samuel D. M. "Activation of multiple hemopoietic growth factor genes in Abelson virus transformed myeloid cells." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27786.
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The stringent requirement for hemopoietic growth factors (HGF) in the induction of hemopoiesis in vitro has raised questions as to their possible role(s) in leukemogenesis. Several recent clinical studies have shown aberrant cell growth factor gene activation in patient derived leukemic cells. Assessment of growth factor activity is often based on in vitro bioactivity assays of conditioned media or body fluids. The specificity of this type of endpoint is, however, open to question due to the overlap in biological activities of many HGFs.
In assessing the role of growth factor gene expression in a murine myeloid leukemia model I have used a sensitive RNA detection procedure coupled with a vector-probe system that enables the synthesis of uniformly labelled radioactive DNA probes to detect unambiguously the expression of particular growth factor genes. The Abelson murine leukemia virus (A-MuLV) derived myeloid transformants used in this study had previously been shown to produce a multi-lineage colony stimulating activity (CSA). While these A-MuLV transformants were shown to produce GM-CSF, it seemed likely that the multi-lineage CSA was due to another factor. In addition to confirming the expression of GM-CSF mRNA, I was able to show that the cells of all four A-MuLV transformed lines tested also expressed interleukin-3 mRNA. This finding was strongly corroborated by bio-activity data obtained using the CM from the A-MuLV myeloid transformants. Additional preliminary analysis by bioactivity assays have also shown the possible presence of interleukin-6 (IL-6) and a recently described pre-B cell factor suggesting perhaps a common mechanism underlying the activation of these various growth factor genes.Medicine, Faculty of
Medical Genetics, Department of
Graduate
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Roberts, April M. "Steroid hormone treatments alter growth characteristics in transformed human ovarian cell lines." Virtual Press, 2003. http://liblink.bsu.edu/uhtbin/catkey/1265095.
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Liu, Chi 1963. "Transforming growth factors produced by normal and neoplastically transformed rat liver epithelial cells in culture." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63768.
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SIPES, NANCY JO. "GROWTH REGULATION OF HUMAN MELANOMA: FACTORS INVOLVED IN THE EXPRESSION OF THE TRANSFORMED PHENOTYPE (SOFT AGAR, GROWTH FACTORS, PLATELETS, ENDOTHELIAL CELLS, PARACRINE)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183788.
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Cellular transformation is accomplished in vitro through the concerted action of growth factors and oncogenes. This association has demonstrated that malignant growth results from aberrations in pathways that normally operate to control proliferation. Activation of genes that code for growth factors, their receptors, and/or molecules essential in the transduction of signals from the cell surface to the nucleus are all potential mechanisms by which tumor cells could establish a selective growth advantage over normal cells. This dissertation addresses the question of what oncogenic mechanisms are important in the development and progression of human melanoma. These studies show that melanoma growth is regulated by endogenous substances produced by the melanoma cells themselves (autocrine stimulation), as well as by exogenous substances supplied by neighboring cells and platelets (paracrine stimulation). These factors work to drive the expression of the transformed phenotype for melanoma as evidenced by induction of serum-free soft agar growth. Human platelets were found to the the richest source of paracrine growth promoters. The factor from human platelets was characterized and partially purified. Melanoma cells respond to this 60,000 molecular weight, disulfide-bond-containing protein in colony formation assays. In addition, the protein has endothelial cell growth factor activity. Purified fractions which promoted optimal colony formation for human melanoma cells also maximally stimulated monolayer growth of bovine aortic endothelial cells, while melanocytes were nonresponsive. This implies that melanoma cells are expressing receptors for a protein which plays no known or apparent role in the normal growth of melanocytes. Melanoma cells are sensitive to growth regulatory molecules of autocrine and paracrine nature. This dissertation provides clues to the genetic lesions which have occurred in these melanoma cells to influence their proliferation. The aberrations appear to reside in those genes important in growth factor pathways at the level of endogenous production and misguided response to exogenous factors through receptor expression. We can not hope to fully inhibit the proliferation of tumor cells until we identify and understand those forces which drive their growth. These studies have increased our knowledge of those signals which stimulate melanoma cellular proliferation, and thus provide insight into important therapeutic targets.
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Taglienti, Cherie A. "Signal Transduction by the EGF Receptor in Normal and Transformed Cells: a Dissertation." eScholarship@UMMS, 1996. http://escholarship.umassmed.edu/gsbs_diss/314.
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Much research has been conducted and significant progress has been made in understanding how signals are transmitted in response to extracellular stimuli. Continued research is necessary to elucidate how specificity in signaling from the cell surface is achieved and to understand how disruption of such signaling pathways leads to disease. The question of specificity is important since many of the same proteins can be activated by different stimuli in a variety of signaling pathways. One means of achieving specificity would be for different cell types to express different sets of proteins that interact with "common" proteins such as GRB2, Shc, and Ras. There were two goals to my dissertation research. The first goal was to study disease which results from perturbation of the EGF receptor. The second was to explore the possibility that signals generated in response to EGF or other stimuli might be transmitted through MAP kinase proteins other than ERKs 1 and 2.
Chapter II of this thesis centers around further analyzing the disease potential of erbB in chickens. I have conducted oncogenicity tests with an erbB oncogene that contains no mutations, only the characteristic N-terminal truncation. My studies have revealed that truncation of the EGF receptor (erbB) can induce tumorigenesis of the kidney as well as the erythroblastosis originally associated with erbB. Previous studies have only found kidney tumors in association with mutated forms of the erbB oncogene. Furthermore, I have demonstrated that mutational removal of a negative regulatory serine phosphorylation site increases the number of wing web tumors caused by the erbB oncogene.
Chapter III describes the identification of the Ser/Thr protein kinase p56 KKIAMRE and characterization of p56 KKIAMRE and p42 KKIALRE. These kinases have homology to the MAP kinase group and contain the conserved Thr-Xaa-Tyr dual phosphorylation site. I have demonstrated that both kinases can be activated by treatment of cells with EGF. Interestingly, phosphorylation of Thr and Tyr in the Thr-Xaa-Tyr motif is not necessary for EGF stimulated activity.
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Mißbach, Sven. "Untersuchungen zu standortunabhängigen Eigenschaften des Wachstums für verschiedene Baumarten." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-175061.
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Towler, Melissa J. "Effects of inoculum density, carbon concentration, and feeding scheme on the growth of transformed roots of Artemisia annua in a modified nutrient mist bioreactor." Digital WPI, 2005. https://digitalcommons.wpi.edu/etd-dissertations/269.
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Previous work has shown that despite the lack of oxygen limitation, transformed roots of Artemisia annua had lower biomass productivity in a nutrient mist bioreactor than in a liquid-phase bubble column reactor where the roots demonstrated metabolic signs of oxygen stress. Mathematical modeling suggested that the roots were too sparsely packed to capture mist particles efficiently and to achieve high growth rates. In this study, higher packing fractions were tested, and the growth rate increased significantly. Similarly, higher sucrose concentrations increased the growth rate. Growth kinetics for 2, 4, and 6 days showed an unexpected decrease or stationary growth rate after only 4 days for both 3% and 5% sucrose feeds. Residual media analyses indicated that carbon was not exhausted, nor were other major nutrients including phosphate. Increasing the misting frequency such that the total amount of carbon delivered from a 3% sucrose feed was equivalent to that delivered in a 5% sucrose feed showed that growth was affected by the modified cycle. These studies showed that both the concentration of carbon source and alteration of misting frequency can significantly increase growth rates of hairy roots in mist reactors.
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Zinn, Jennifer Nicole. "Studying modulation of tumor necrosis factor-Ã and transforming growth factor-ß by norepineprhine in murine alveolar macrophages, murine splenocytes and transformed cell populations." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ33833.pdf.
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Shoji(kawata), Sanae. "p21Waf1/Cip1/Sdi1 functions to prevent apoptosis as well as stimulate growth in cells transformed or immortalized by human T-cell leukemia virus type 1-encoded Tax." Kyoto University, 2003. http://hdl.handle.net/2433/148476.
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Hafermann, Juliane O. [Verfasser], and Martin [Akademischer Betreuer] Löchelt. "The effect of APOBEC3A-mediated mutagenesis on tumour growth of immortalised, transformed cells and on survival of head and neck squamous cell carcinoma patients / Juliane O. Hafermann ; Betreuer: Martin Löchelt." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1177043645/34.
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Hafermann, Juliane O. Verfasser], and Martin [Akademischer Betreuer] [Löchelt. "The effect of APOBEC3A-mediated mutagenesis on tumour growth of immortalised, transformed cells and on survival of head and neck squamous cell carcinoma patients / Juliane O. Hafermann ; Betreuer: Martin Löchelt." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1177043645/34.
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LIMA, FILHO Luiz Medeiros de Araújo. "Modelos simétricos transformados não-lineares com diferentes distribuições dos erros: aplicações em ciências florestais." Universidade Federal Rural de Pernambuco, 2009. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5175.
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Historically, the wood of the eucalyptus is used for the most varied applications, such as; firewood, charcoal, cellulose, railway sleepers, posts for electrification, bark to tan leather, essential oils, civil construction, etc. The Gypsum Pole of Araripe in Pernambuco is a great firewood consumer for the gypsum production. Due to great need to find economical and environmental alternatives for the area, the sustainable production of eucalyptus that is a fast growth tree with great versatility has an important role. In the planning of the sustainable forest management there is a variable of extreme importance: the growth. To model the growth is fundamental in the prognosis of the productivity, site quality and dynamics of populations. Usually, the growth curves are fitted through nonlinear models developed empirically to relate, for instance, height and age. The Chapman-Richards model is a nonlinear model frequently used to model forest growth. In studies of this type, in general, it is assumed that the errors follow approximately the normal distribution. However, to model the growth assuming that the errors have a normal distribution is quite sensitive to atypical values that can happen, and generate bad estimates of the parameters. To correct that problem a new class of transformed symmetrical models was developed considering for the errors symmetrical continuous distributions with heavier tails than the normal distribution and allowing a possible nonlinear structure for the mean. With the expectation of obtaining better estimates of eucalyptus growth, it was applied to the Chapman-Richards model the following distributions of the errors: normal, t of Student, Cauchy, exponential potency, logistics I and logistics II. The t distribution of Student with 2 degrees of freedom was the most efficient to estimate height and circumference growth of eucalyptus in the Gypsum Pole of Pernambuco.
Historicamente, a madeira do Eucalyptus é usada para os mais variados fins, tais como; lenha, carvão vegetal, celulose, dormentes ferroviários, postes para eletrificação, casca para curtir couro, óleos essenciais, construção civil, etc. O Pólo Gesseiro do Araripe em Pernambuco é um grande consumidor de madeira para produção de gesso. Devido à grande necessidade de se buscar uma alternativa econômica e ambiental para a região é de interesse obter uma produção sustentável para o Eucalyptus, uma vez que esta é uma árvore de rápido crescimento e grande versatilidade. No planejamento do manejo florestal sustentado uma variável é de extrema importância: o crescimento. Sua modelagem é fundamental na prognose da produtividade, qualidade do local e dinâmica de populações. Geralmente, as curvas de crescimento são estudadas por meio de modelos não-lineares desenvolvidos empiricamente para relacionar, por exemplo, altura e idade. Um modelo não-linear bastante utilizado na prática para modelar curvas de crescimento é o modelo de Chapman-Richards. Em estudos deste tipo, em geral, assume-se que os erros seguem distribuição normal. Contudo, a modelagem sob a suposição de erros com distribuição normal é bastante sensível a valores atípicos que por ventura possam ocorrer, podendo distorcer as estimativas dos parâmetros. Para corrigir esse problema Cordeiro et al. (2009) desenvolveram uma nova classe de modelos simétricos transformados considerando para os erros distribuições contínuas simétricas com caudas mais pesadas do que a distribuição normal e permitindo uma possível estrutura não-linear para a média. Dessa forma, com a expectativa de obter melhores estimativas de crescimento de Eucalyptus, aplicaram-se ao modelo de Chapman-Richards as seguintes distribuições dos erros: normal, t de Student, Cauchy, exponencial potência, logística I e logística II que apresentou a distribuição t de Student com 2 graus de liberdade com melhores estimativas de crescimento em altura e circunferência de Eucalyptus no Pólo Gesseiro de Pernambuco.
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Srivastava, Sachi. "Laplace transforms, non-analytic growth bounds and C₀-semigroups." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249507.
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In this thesis, we study a non-analytic growth bound $\zeta(f)$ associated with an exponentially bounded measurable function $f: \mathbb{R}_{+} \to \mathbf{X},$ which measures the extent to which $f$ can be approximated by holomorphic functions. This growth bound is related to the location of the domain of holomorphy of the Laplace transform of $f$ far from the real axis. We study the properties of $\zeta(f)$ as well as two associated abscissas, namely the non-analytic abscissa of convergence, $\zeta_{1}(f)$ and the non-analytic abscissa of absolute convergence $\kappa(f)$. These new bounds may be considered as non-analytic analogues of the exponential growth bound $\omega_{0}(f)$ and the abscissas of convergence and absolute convergence of the Laplace transform of $f,$ $\operatorname{abs}(f)$ and $\operatorname{abs}(\|f\|)$. Analogues of several well known relations involving the growth bound and abscissas of convergence associated with $f$ and abscissas of holomorphy of the Laplace transform of $f$ are established. We examine the behaviour of $\zeta$ under regularisation of $f$ by convolution and obtain, in particular, estimates for the non-analytic growth bound of the classical fractional integrals of $f$. The definitions of $\zeta, \zeta_{1}$ and $\kappa$ extend to the operator-valued case also. For a $C_{0}$-semigroup $\mathbf{T}$ of operators, $\zeta(\mathbf{T})$ is closely related to the critical growth bound of $\mathbf{T}$. We obtain a characterisation of the non-analytic growth bound of $\mathbf{T}$ in terms of Fourier multiplier properties of the resolvent of the generator. Yet another characterisation of $\zeta(\mathbf{T}) $ is obtained in terms of the existence of unique mild solutions of inhomogeneous Cauchy problems for which a non-resonance condition holds. We apply our theory of non-analytic growth bounds to prove some results in which $\zeta(\mathbf{T})$ does not appear explicitly; for example, we show that all the growth bounds $\omega_{\alpha}(\mathbf{T}), \alpha >0,$ of a $C_{0}$-semigroup $\mathbf{T}$ coincide with the spectral bound $s(\mathbf{A})$, provided the pseudo-spectrum is of a particular shape. Lastly, we shift our focus from non-analytic bounds to sun-reflexivity of a Banach space with respect to $C_{0}$-semigroups. In particular, we study the relations between the existence of certain approximations of the identity on the Banach space $\xspace$ and that of $C_{0}$-semigroups on $\mathbf{X}$ which make $\mathbf{X}$ sun-reflexive.
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Salim, Sateria. "In-situ Fourier transform infrared spectroscopy of chemistry and growth in chemical vapor deposition." Thesis, Massachusetts Institute of Technology, 1995. http://hdl.handle.net/1721.1/11378.
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Gwanzura, Zvikomborero Takunda. "Fabrication of a gold nanorod metal organic framework sensor for epidermal growth factor ; a biomarker for kidney disease." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2778.
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Thesis (Master of Applied Sciences in Chemistry)--Cape Peninsula University of Technology, 2018.Biosensors have been on the forefront to provide clinical diagnosis tools for various diseases. Proper selection of biomarkers as well as chemical electrode modification is key in the fabrication of electrochemical biosensors. Hence, electrode modified with nanomaterials devices to improve electroanalytical applications. These nanomaterials were functionalized to improve conductivity, accelerate signal transduction and amplify biorecognition events. Thus, resulting in novel sensing platforms that are highly sensitive and selective towards the target analyte. In this study, gold nanorods (Au NRs) capped with CTAB, zeolitic imidazole framework were synthesised using the seed mediated and hydrothermal method respectively. Composites of gold nanorods with cysteine, ZIF-8 or both were also synthesised. All synthesised materials were characterized using ultraviolet–visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-Ray diffraction (XRD) and cyclic voltammetry (CV) techniques. The obtained results confirmed the synthesis of the nanomaterials and composites. Identification of the ideal platform for fabrication of a transducer with the best electrochemical response was determined by studying the combinations of the synthesised nanomaterials and composites. The studied parameters were surface coverage, conductivity, rate of electron transfer constant. Cysteine-Au NRs composites platforms, had exceptional properties hence its synthesis optimisation of was undertaken. The effect of CTAB, reaction time, volume and concentration ratio of Au:Cysteine, temperature and pH on the composite properties were assessed. However, this composite’s electrochemical properties decreased when bioconjugated with the antibodies. Hence, the choice of Au NRs CTAB functionalised ZIF-8 (CTABAu/ZIF-8) as the transducer for biosensor applications due to a more favourable biocompatibility. Biosensor fabrication was done by drop coating glassy carbon electrode with the CTABAu/ZIF-8 forming a transducer followed by immobilisation of the antibody (Ab) using a covalent attachment method with glutaraldehyde (GA) as a cross linker. The target analyte, epidermal growth factor (EGF) was interacted with the Ab binding sites via electrostatic forces. All the fabrication steps were optimized for biosensor components, immobilization technique (drop coating and immersion), concentration and incubation time of linker and bioreceptor, as well as the synthesis of the CTABAu-ZIF-8 composites where in situ and ex situ techniques were compared together with the effect of the concentration ratio of Au: ZIF-8. There was also an analysis of optimum pH. Optimum conditions were found to be immersion in 3 % GA and 2 μg/ml Ab, with incubation times of 8, 10 and 5 minutes for GA, Ab and EGF respectively at a pH of 6. The following electroanalytical techniques: cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) were utilised for EGF detection. The DPV showed better reproducibility, higher currents and better resolution hence; it was the method of choice. The technique’s optimisation involved assessments of the effect of step potential, starting potential and pulse amplitude. The optimum response for pulse amplitude, step potential and starting potential were 60 mV, 20 mV and 0.5 V respectively. The biosensor analytical parameters were linear towards EGF in the concentration range from 2 to 100 nM with a detection limit of 0.58 nM. Reproducibility and repeatability tests were acceptable, and the biosensor had a stability over 80 % within 15 days. There was no interference observed in the presence of glucose and creatine. The EGF biosensor was successfully applied in urine and saliva analysis, obtaining 67.5 and 3.12 nM respectively. This biosensor’s positive outcome strongly suggests its potential as a diagnosis tool for early detection of kidney disease as it was able to detect EGF concentration within physiological levels of EGF in normal kidney function.
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Wen, Xin Li. "Étude d'une lignée de cellules de hamster transformées chimiquement capables de croitre sans ancrage dans un milieu sans sérum ni mitogènes." Paris 11, 1985. http://www.theses.fr/1985PA112001.
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Cette thèse présente les résultats de l'étude de fibroblastes d'embryon de hamster transformés par un cancérogène chimique (cellules BA 10-IR). Cette lignée est très tumorigène, et pousse indéfiniment dans un milieu synthétique sans sérum ni facteurs mitogènes (S⁻/M⁻). Les cellules BA10-IR ont aussi la propriété, non encore décrite, de croître en l'absence d'ancrage en milieu S⁻/M⁻ liquide ou semi-solide. Cette capacité va de pair avec la production d'un TGF (transforming growth factor), auquel les cellules BA10-IR sont sensibles. Un TGF similaire est produit par les fibroblastes d'embryon de hamster témoins (FEH), mais ils n'y répondent pas. Les FEH ne poussent pas non plus en milieu S⁻/M⁻. Les fibroblastes de rat FR3T3 transformés par le Ki-MSV sont sensibles aux TGFs des cellules BA10-IR et FEH, et croissent en l'absence d'ancrage en milieu S⁻/M⁻ en leur présence. Mais, ils ne produisent pas de TGF stimulant leur croissance en milieu S⁻/M⁻. Le phénotype unique des cellules BA10-IR ne semble pas être dû à l’activation des proto-oncogènes cellulaires h-ras et myc. Ces proto-oncogènes ne sont pas réarrangés dans l’ADN des cellules BA10-IR et sont exprimés à des niveaux comparables dans ces cellules et les FEH témoins.
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Landis, Tamra R. "How a Successful Collecting Society Can Transform an Art Museum: A History of The Georgia Welles Apollo Society at the Toledo Museum of Art." Bowling Green State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1522759729069838.
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Antonysamy, Alphons Anandaraj. "Microstructure, texture and mechanical property evolution during additive manufacturing of Ti6Al4V alloy for aerospace applications." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/microstructure-texture-and-mechanical-property-evolution-during-additive-manufacturing-of-ti6al4v-alloy-for-aerospace-applications(03c4d403-822a-4bfd-a0f8-ef49eb65e7a0).html.
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Additive Manufacturing (AM) is an innovative manufacturing process which offers near-net shape fabrication of complex components, directly from CAD models, without dies or substantial machining, resulting in a reduction in lead-time, waste, and cost. For example, the buy-to-fly ratio for a titanium component machined from forged billet is typically 10-20:1 compared to 5-7:1 when manufactured by AM. However, the production rates for most AM processes are relatively slow and AM is consequently largely of interest to the aerospace, automotive and biomedical industries. In addition, the solidification conditions in AM with the Ti alloy commonly lead to undesirable coarse columnar primary β grain structures in components. The present research is focused on developing a fundamental understanding of the influence of the processing conditions on microstructure and texture evolution and their resulting effect on the mechanical properties during additive manufacturing with a Ti6Al4V alloy, using three different techniques, namely; 1) Selective laser melting (SLM) process, 2) Electron beam selective melting (EBSM) process and, 3) Wire arc additive manufacturing (WAAM) process. The most important finding in this work was that all the AM processes produced columnar β-grain structures which grow by epitaxial re-growth up through each melted layer. By thermal modelling using TS4D (Thermal Simulation in 4 Dimensions), it has been shown that the melt pool size increased and the cooling rate decreased from SLM to EBSM and to the WAAM process. The prior β grain size also increased with melt pool size from a finer size in the SLM to a moderate size in EBSM and to huge grains in WAAM that can be seen by eye. However, despite the large difference in power density between the processes, they all had similar G/R (thermal gradient/growth rate) ratios, which were predicted to lie in the columnar growth region in the solidification diagram. The EBSM process showed a pronounced local heterogeneity in the microstructure in local transition areas, when there was a change in geometry; for e.g. change in wall thickness, thin to thick capping section, cross-over’s, V-transitions, etc. By reconstruction of the high temperature β microstructure, it has been shown that all the AM platforms showed primary columnar β grains with a <001>β.
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ENOKIHARA, CYRO T. "Estudo do quartzo verde de origem hidrotermal tratado com radiação gama." reponame:Repositório Institucional do IPEN, 2013. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10600.
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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Johansson, Anders. "Search for biomarkers in ALS and Parkinson's Disease positron emission tomography and cerebrospinal fluid studies /." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univeritetsbiblioteket [distributör], 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-102040.
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HIGUTI, ELIZA. "Correção fenotípica do nanismo avaliada por diferentes parâmetros de crescimento após administração de DNA plasmidial em modelo animal de deficiência isolada do hormônio do crescimento." reponame:Repositório Institucional do IPEN, 2016. http://repositorio.ipen.br:8080/xmlui/handle/123456789/26374.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Tese (Doutorado em Tecnologia Nuclear)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
FAPESP:11/21708-6
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Dezillie, Britta. "Étude de la tenue aux radiations de détecteurs de particules en silicium épitaxial pour leurs utilisaitons au LHC du CERN." Université Joseph Fourier (Grenoble ; 1971-2015), 1997. http://www.theses.fr/1997GRE10146.
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Dans les experiences futures dans le lhc de cern on veut utiliser des detecteurs de silicium de haute precision. Le composent hadronique influence le temps de vie des detecteurs. La tenue aux irradiations du silicium peut etre amelioree par l'etude des defauts. Cette etude implique l'addition des impuretes au silicium afin de former des centres de defauts qui sont actives d'une maniere electrique. Ceux ci controlent les parametres macroscopiques des echantillons. Les ingredients principales a examiner sont l'oxygene et le carbone parce qu'ils se comportent comme consommateurs des vacances et des interstitials. Un premier essai a introduire du ge dans le silicium a ete realise. Du materiel epitaxiale du type n de macom (us) qui contient dix fois plus d'oxygene que le float-zone (fz), montre une amelioration d'un facteur 2 a l'egard du fz. Les impuretes initiales au sein du materiel ont ete mesurees par sims et spectrometrie infrarouge (ir). Les etudes d'irradiations des detecteurs epitaxiales du type n et p avec une epaisseur de 200 m, produits par itme (pologne) avec differentes vitesses de croissance, sont reportees dans ce travail. Une comparaison de differents procedes comme l'implantation par ionisation et mesa a ete faite. La tenue aux irradiations des detecteurs de silicium epitaxials avec des resistivites initiales de 25 ohm cm a 10 kohm cm a ete comparee avec du fz standard. Les resultats sont presentes en terme de la variation du courant de fuite, la concentration de dopage effectif et la collection de charge en fonction de la fluence et le temps apres des irradiations proton en neutron jusqu'une fluence de 4. 5 10#1#4 p/cm#2.
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Wolf, Jörg. "Regularität schwacher Lösungen nichtlinearer elliptischer und parabolischer Systeme partieller Differentialgleichungen mit Entartung." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2002. http://dx.doi.org/10.18452/14792.
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In der vorliegenden Arbeit untersuchen wir schwache Lösungen, die zu einem geeigneten Sobolevraum gehören, q-elliptischer und parabolischer Systeme partieller Differentialgleichungen auf deren Regularität für den Fall 1In the present work we study the regularity of weak solution to q-elliptic and parabolic systems partial differential equations in appropriate Sobolev spaces in case 1
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楊梅如. "Effect of TGF-β on the Growth of Transformed Uterine Cells." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/96959967470241683946.
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碩士國立成功大學
生理學研究所
83
The uterus undergoes growth and regression which is associated with the cyclic changes of reproductiong cycle in females. Femal sex steroids and some growth factors are known to affect the growth of the uterus. TGF-B is one of these factors. Since the tissure growth is the result of dynamic balance between cell proliferation and death, this study is to investigate the effect of TGF-B on cell proliferation and death. We used HRE-H9 cells, rabbit uterine epithelial cells tr nsformed by temperature-sensitive mutant SV40 virus, to study how TGF-B affects the growth of transformed uterine cells. Cells in culture were treated with TGF-B, then the changes in cell number, cell cycle and 3H-thymidine incorporation were evaluated. In addition, apoptotic death was characterized by morphological and biochemical methods. Our results showed that, in unsynchronized cells, the treatment with TGF-B inhibited cell proliferation and suppressed DNA synthesis. The simultaneous treatment with femals sex steroids and TGF-B attenuated the inhibitory effect. The cell cycle distribution analysis showed that the percentage of S phase decreased and the percentage of G2/M phase increased after TGF-B treatment. A typical DNA laddering pattern of apoptosis appeared in the TGF-B treated cells. In the sychronized cells by serum starvation, TGF-B inhibited cell proliferation and increased the percentage of DNA fragmentation. When cells were synchronized with 1mM hydroxyurea, we observed that TGF-B suppressed the increase in cell number after 1 day treatment. But cell cycle distribution and DNA synthesis duing the same period was not affected, Morphological analysis by Hoechst staining demonstrated that apoptotic death increased significantly in the first day of TGF-B treated. Biochemical analysis further showed that a significant increase in DNA fragmentation occurred at 12 hours posttreatment, which was equivalent to the peak of G2/M phase. Gel electrophoresis also showed that DNA fragments appeared 3 hours posttreatment of TGF-β. These results indicated that TGF-B affected the growth of transformed uterine cells by two functions: TGF-B might suppress cell cycle progressing from G1 phase to S phase and inhibited DNA synthesis; TGF-B also increased the apoptosis percentage with culturing time. The apoptosis occured during cell cycle progression.
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Feng, Hsing-Chi, and 馮幸琪. "Epidermal Growth Factor (EGF) Suppressed Apoptotic Death in Transformed Uterine Epithelial Cells." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/31910367039198246005.
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碩士國立成功大學
生理學研究所
87
Animal studies have shown that estrogen stimulates proliferation of uterine epithelial cells and inhibits cell death . Considerable evidence suggests that the effects of estrogen on the uterine cells may be mediated indirectly through growth factors such as epidermal growth factor (EGF). However, there is no study related to the effect of growth factor on uterine cell death. In this study, a temperature-sensitive SV40 mutant transformed rabbit uterine epithelial cell line was used to investigate the effect of EGF on cell death. Our results showed that cells grew well in 4% fetal calf serum. When they were changed to serum free medium, the apoptotic death was observed with the appearance of DNA ladders. The treatment with EGF increased cell number and decreased DNA fragmentation. To detect the extent of cell death, cells were incubated with 3H-thymidine for 48 hours and the retention of 3H-thymidine incorporated into DNA was then measured. 3H-thymidine retention decreased with time after serum deprivation and EGF could reduce cell death dose-dependently. EGF induced the phosphorylation of EGF receptor (EGFR) of H9 cells. The treatment with AG1478, a blocker of EGFR, reduced EGFR phophorylation and also abolished the effect of EGF on cell death. The treatment with PMA, the activator of protein kinase C (PKC), enhanced cell survival in serum-free medium. GF109204X, which is a blocker of PKC reduced the effect of PMA and EGF on cell death. In contrast, the treatment with LY294002 and wortmannin (PI3-kinase inhibitor) or PD98059 (MEK1 inhibitor) could not inhibit the effect of EGF. Our results suggest that the PKC pathway may be involved in the action of EGF on preventing cell death.
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Claeyssen, Eric. "Growth and respiration of maize suspension cells transformed with a barley phytoglobin gene." 2002. http://hdl.handle.net/1993/19619.
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Huang, Shiao-Wei, and 黃曉薇. "Growth and differentiation of carp maternal gene transformed human pituitary gland tumor cells." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/59331900357990292828.
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碩士國立臺灣大學
動物學系
81
Human pituitary gland tumor cells (Hpt-1) were transfected with a maternal gene from carp egg. A transfectant cell line Hp-3 lost the ability to growth in soft agar. Immunoprecipitation and western blot analysis revealed that in Hp-3 the protein amount of epidermal growth factor receptor (EGFR) was considerably reduced as compared with the parental Hpt-1 cells, on the other hand that of glial fibrillary acidic protein (GFAP) and p53 were increased. Furthermore we found that Hp-3 cells could adhere and proliferate in bacteriological dish better than Hpt-1 cells, which suggested that Hp-3 cells secret a lot more of extracellular matrix into the medium. The result of western blot analysis indeed showed that the protein level of fibronectin of Hp-3 cell grown in bacteriological dishes was increased as compared with that in tissue culture dishes. A growth-stimulating activity was found in the serum- free medium conditioned by Hpt-1 or Hp-3 cells, which promoted the proliferation of hepatoma (NTU-BL), Hela, NIH 3T3 and human fibroblast cells except Hpt-1 and Hp-3 cells themselves. And there was no significant difference in the growth-stimulating activity between Hpt-1 and Hp-3 conditioned media. Hela and 3T3 were chosen as the main assay systems and exhibited a dose- dependence of mitotic response to the added conditioned medium. Since basic fibroblast growth factor (bFGF) has been examined as an autocrine regulator of glioma cell growth and a paracrine angiogenic factor facilitating its neoplastic progression, 0.5 ug /ml of anti-human bFGF antibody was added to the conditioned media of Hpt-1 and Hp-3 cells. However, the growth-stimulating activity was still not neutralized, suggesting that the mitogenic activity was not from bFGF.
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王家昌. "= Growth charcteristics of transformed stizolobium hassjoo hairy root and operation strategies for mist tricklingbioreactor." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/98283100037093421865.
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鄭琮霖. "Anti-apoptotic effects of basic fibroblast growth factor(bFGF) on transformed uterine epithelial cells." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/39724800297474565707.
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碩士國立成功大學
生理學研究所
89
Basic fibroblast growth factor （bFGF）, a member of FGF family, stimulates cell proliferation, differentiation and survival in a variety of cell types. So far, there is no information related to the action of growth factors on cell survival in uterine epithelial cells yet. This study was to investigate the mechanisms by which bFGF regulates the survival of uterine epithelial cells. A temperature-sensitive SV40 mutant transformed rabbit uterine epithelial cell line was used. Cells were cultured in a medium containing 4% fetal calf serum and 3H-thymidine for 48 hours and then shifted into 3H-thymidine-free and serum-free medium in the presence or absence of bFGF. The retention of incorporated 3H-thymidine was measured as an index of cell survival. The results show that serum deprivation causes a decrease of retained 3H-thymidine in cultured cells and bFGF treatment prevents the loss of incorporated 3H-thymidine. Furthermore, electrophoresis analysis shows that serum deprivation induces apoptosis, which is characterized by the appearance of DNA ladders. The treatment with bFGF decreases fragmented DNA ladders. The treatment of protein kinase C（PKC） inhibitor（GF10923X） and phospholipase C（PLC） inhibitor（U73122）abolish the anti-apoptotic effect of bFGF. However, MEK inhibitor（PD98059 and U0126）or PI3-kinase inhibitor（LY294002） is ineffective on bFGF action. Additionally, Western blot analysis shows that bFGF can stimulate the translocation of PKCalpha from the cytoplasm to membrane. PLC inhibitor or tyrosine kinase inhibitor（genistein） inhibits the translocation of PKCalpha and also abolishes retention of 3H-thymidine in cells treated with bFGF. These results indicate that bFGF has anti-apoptotic effect on uterine epithelial cells through the activation of PKC.
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(7042784), Mohammed S. Alyakoob. "The Economics of Geographic and Demographic Heterogeneity in Digitally Transformed Markets." Thesis, 2019.
Find full textAbstract:
The digital transformation of markets can remove traditional geographic restrictions, democratizing access to previously unattainable products, and enable individuals to extract rent from their personal assets. However, these digital innovations often have competitors and complementors that are not immune to the impact of local factors such as the local market structure, economic condition, and even demographics. This dissertation examines the geographic and demographic heterogeneity driven disparities in two digitally transformed markets, the financial and accommodations sectors respectively.
First, we study the impact of local financial market competition in managing online peer-to-peer loans. With the boom of financial technologies (FinTech), a critical question is whether the local financial market structure still matters. Unlike traditional retail financial institutions that are predominantly territorial, FinTech-based platforms, in particular peer-to-peer (P2P) lending, provide individuals equal access to funds by removing typical geographic restrictions. Combined with other benefits such as ease-of-use and lower interest rates, P2P lenders are increasingly threatening the traditional local lenders. A largely unanswered question in the literature is whether the local retail financial institutions strategically respond to the rise of such P2P platforms. Moreover, if the strategic reaction of traditional institutions continues the legacy of being territorial, borrowers will ultimately gain unevenly from the competition. That is, where a borrower lives may still matter. In this chapter, we devise multiple strategies to empirically analyze the extent and nature of the strategic response of traditional institutions to P2P lending. This includes: (1) utilization of a Probit model that leverages the richness of our local market data and (2) exploitation of bank mergers as exogenous shocks to local market structure. We find consistently that a borrower from a more competitive market is more likely to prepay, suggesting that local market structure plays a pivotal role in P2P borrowers' debt management. We validate the underlying mechanism by studying the improving credit profiles of borrowers and platforms' (exogenous) changes in pricing in moderating the main effect. This mechanism reveals that traditional banks, especially when their local market conditions support, credibly responds to the growth of P2P and are successful in attracting consumers back to traditional financial products. Relatedly, we document heterogeneity in the benefits that borrowers gain from the local market structure (using a machine learning algorithm) and verify the robustness of our main findings. We discuss the implications for P2P lending, other crowd-based markets, and local retail financial markets.
Second, we examines the heterogeneous economic spillover effects of a home sharing platform---Airbnb---on the growth of a complimentary local service---restaurants. By circumventing traditional land-use regulation and providing access to underutilized inventory, Airbnb is attracting visitors of a city to vicinities that are not traditional tourist destinations. Although visitors generally bring significant spending power, it is, however, not clear if the visitors use Airbnb primarily for lodging, thus, not contributing to the adjacent vicinity economy. To evaluate this, we focus on the impact of Airbnb on the restaurant employment growth across vicinities in New York City (NYC). Our results indicate that if the intensity of Airbnb activity (Airbnb reviews per household) increases by 1\%, the restaurant employment in an average area grows by approximately 1.03\%. We also investigate the role of demographics and market concentration in driving the variation. Notably, restaurants in areas with a relatively high number of Black residents do not benefit from the economic spillover of Airbnb activity. Also, restaurants in more competitive areas reap the benefit from this spillover most. We validate the underlying mechanism behind the main result by evaluating the impact of Airbnb on Yelp visitor reviews -- areas with increasing Airbnb activity experience a surge in their share of NYC visitor reviews. This result is further validated by evaluating the impact of a unique Airbnb neighborhood level policy recently implemented in New Orleans.
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Luo, Hong Ren, and 羅宏仁. "Study on the growth factors required by human epithelial cells transformed by human papillomavirus type 16 DNA." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/97171639747082109019.
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碩士國立臺灣大學
生化學研究所
83
NW-1 cells of normal human nasal epithelial cells in origin were immortalized by human papillomavirus type 16 DNA. The growth of NW-1 cells require insulin (Ins), epidermal growth factor(EGF)and bovine pituitary extract (BPE). BPE alone does not stimulate DNA synthesis, but acts in synergy with Ins or EGF to promote G2/M transition in NW-1 cells. In combination with Ins,but not EGF,BPE also had a slight effect on promotion of G2/M transition in Xenopus oocytes. Four growth-stimulating polypeptides of 17 kilodaltons,and pI ranged between 6.6 and 7.0 were purified from BPE. Amino acid sequences at the N terminals of these polypeptides were highly homologous to the a or b subunit of bovine hemoglobin (HB). Hb was found growth-stimulating even at the concentraction of 0.1 ng/ml. Hb when subjected to the identical purification protocal of BPE resulted in the purified Hb which was found also growth-stimulating to NW-1 cells. Therefore, why substances with sequence similar to alpha or beta subunit of Hb can act like mammalian growth factors is significant to find out.
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Hawthorne, Rhonda. "A teacher's journey to transform her math identity." 2017. http://hdl.handle.net/1993/32014.
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This study explored the transformation of a teacher’s math identity. The researcher used narrative inquiry to live alongside the participant (Clandinin, 2013) as she experienced teaching in a pedagogically courageous way to make math meaningful to her students. Along the way the participant experienced cognitive dissonance but through intense collaborative professional reflection she persevered and evolved. The study addressed the questions: What factors influence a teacher’s motivation to grow and to make math meaningful to their students? What factors influence a teacher to commit to lifelong learning in math? Four themes emerged through the observations, journals, and reflective conversations including sharing of interim texts and the final narrative. The importance of the relationship with the researcher/coach, the trust that the participant had in the coach, the process, and herself, her desire for balance in her teaching life, and her improved sense efficacy led to her transformed math identity.February 2017
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Alves, Ludovico Hélder Martins. "Efeito da expressão da isoforma S de Endoglina sobre a migração celular: Análise da mediação das rotas de MAPK no processo." Master's thesis, 2010. http://hdl.handle.net/10400.6/3941.
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A família do factor de crescimento TGF-β regula vários processos, em espécies tão diversas como moscas ou humanos. Desde que foi descoberta, no começo dos anos 80, esta família foi considerada como um importante regulador da proliferação, diferenciação, mobilidade, adesão, organização celular e morte celular programada (Massague 1998). Os membros da família TGF-β desempenham tarefas fundamentais durante o desenvolvimento embrionário e na homeostase dos tecidos em organismos metazoários. Investigação desenvolvida nos últimos anos clarificou sobre o funcionamento da rede de transdução da família de sinais TGF-β. Esta rede envolve receptores ligados a cinase de serina/treonina e os seus substratos, as proteínas Smad, que se deslocam para o núcleo após activação, onde em associação com ligandos do ADN, activam a transcrição de genes específicos. Diferentes classes de receptores, proteínas Smad e ligandos do ADN, permitem, de uma maneira específica para cada célula, que TGF-β possua uma natureza multifuncional (Massague 1998).
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Pudakalakatti, Shivanand. "Novel NMR Methods for Fast Data Acquisition : Application to Metabolomics." Thesis, 2014. http://hdl.handle.net/2005/2797.
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Synopsis My research work is focused on: (i) development of novel Fast NMR methods in solution state and their application to metabolomics and small molecules. (ii) NMR based metabolic study of human IVF to assess embryo viability for implantation. The major components of the embryo growth media were identified for evaluating the embryo quality. Described below are the projects carried out towards the dissertation of my PhD. Chapter 1 describes NMR methods which are the foundation stones for new Fast NMR methods developed. Typical 1D and 2D NMR experiments used in metabolomics and statistical methods for analysis are described. A few applications of metabolomics are also covered in the chapter. Chapter 2 describes a new Fast NMR method based on polarization sharing and parallel acquisition using the dual receiver system. The method developed helps in acquiring simultaneously three 2D NMR spectra: 2D [13C-1H] HETCOR, 2D [1H-1H] TOCSY and 2D [13C-1H] HSQC-TOCSY in a single data set. This method achieves a time saving of about two fold. All the experiments are acquired on molecules with natural abundance of 13C. The method was used to assign the side chain atoms (1H and 13C) of two important peptides. i) 12 amino acid residue peptide, which is a part of central linker domain of Human Insulin like Growth Factor Binding Protein-2 known to play a vital role in the IGF system and ii) a 18 amino acid residue peptide which acts as an antimicrobial agent.
Chapter 3 describes extension of the Fast NMR method described in chapter 2. The method is combined with G-matrix Fourier Transform NMR spectroscopy. In this method we have acquire simultaneously two 2D NMR experiments and one reduced dimensional 3D experiment. The three experiments are 2D [13C-1H] HETCOR, 2D [1H-1H] TOCSY and GFT (3,2)D [13C-1H] HSQC-TOCSY, which provide complementary information for rapid assignments. GFT (3,2)D [13C-1H] HSQC-TOCSY gives 3D correlations in a 2D manner facilitating high resolution and unambiguous assignments. The experiments were applied for complete assignment of 21 unlabeled metabolite mixtures corresponding to the Innovative Sequential medium (ISM1) used for culturing human embryos for IVF. Further, a 13C multiplicity edition block is added to the method to simplify the resonances assignment in GFT (3,2)D [13C-1H] HSQC-TOCSY. Taken together, experiments provide time gain of order of magnitudes compared to conventional data acquisition.
Chapter 4 of the thesis describes a metabolomics study of Human in-vitro fertilization to assess viable embryos of implantation potential using NMR as non-invasive tool. NMR study included the analysis of 127 embryo culture media (Innovative Sequential Media-1) and 29 controls (culture media without embryo) of both day-2 and day-3 transferred. The embryos were divided into 3 categories 1) implanted (successful) 2) transferred not-implanted (unsuccessful) 3) not transferred based on morphological studies. All NMR experiments were acquired with CPMG (T2 filter) incorporated in 1D 1H presaturation pulse scheme. The study was based on estimation of lactate, pyruvate and alanine levels in the embryo culture media (ISM1). The study reveals higher uptake of pyruvate and high pyruvate/alanine ratios in case of implanted embryos compared to one which failed to implant. Present study provides pyruvate/alanine ratio as a biomarker to select the
embryos with high implantation potential. The method combined with morphology based assessment or with other biomarkers can be serve as a powerful tool to assess the embryo quality. Chapter 5 describes a novel NMR method for rapid characterization of translation diffusion of molecules in solution either in mixture or pure form. Unlike acquisition of several 2D [13C-1H] HSQC experiments with varying gradients to get diffusion measurement, a single 2D [13C-1H] HSQC is sufficient to measure the diffusion coefficients which is in the linewidths of peaks. The method uses the idea of accordion NMR spectroscopy, wherein gradients are linearly co-incremented with 13C chemical shift evolution period during t1. The methodology speeds up the acquisition by replacing series of 2D [13C-1H] HSQC with single 2D constant time [13C-1H] HSQC. The method was used to monitor the diffusion of metabolites in a time-resolved manner during polymerization of SDS-PAGE gel. Using this method, it was possible to detect the presence of oligomers of diphenylalanine (FF) during its self assembly to form nanotubular structures.