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Journal articles on the topic 'Transgenic grapevine'

Author: Grafiati

Published: 11 March 2023

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1

Gölles, R., R. Moser, H. Pühringer, et al. "TRANSGENIC GRAPEVINES EXPRESSING COAT PROTEIN GENE SEQUENCES OF GRAPEVINE FANLEAF VIRUS, ARABIS MOSAIC VIRUS, GRAPEVINE VIRUS A AND GRAPEVINE VIRUS B." Acta Horticulturae, no. 528 (May 2000): 307–14. http://dx.doi.org/10.17660/actahortic.2000.528.42.

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2

Yu, Yanyan, Yong Ni, Tian Qiao, et al. "Overexpression of VvASMT1 from grapevine enhanced salt and osmotic stress tolerance in Nicotiana benthamiana." PLOS ONE 17, no. 6 (2022): e0269028. http://dx.doi.org/10.1371/journal.pone.0269028.

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Salt and drought stresses are major environmental conditions that severely limit grape growth and productivity, while exogenous melatonin can alleviate the drought and salt damage to grapevines. N-acetylserotonin methyltransferase (ASMT) is the key enzyme in melatonin synthesis, which plays a critical role in regulating stress responses. However, the roles of ASMTs from grapevine under drought and salt stresses responses remain largely unclear. In this study, the VvASMT1 gene was isolated from grapevine, and its physiological functions in salt and mimic drought stress tolerance were investigat

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3

Gribaudo, I., G. Gambino, S. Leopold, and M. Laimer. "MOLECULAR CHARACTERIZATION OF TRANSGENIC GRAPEVINE PLANTS." Acta Horticulturae, no. 689 (August 2005): 485–92. http://dx.doi.org/10.17660/actahortic.2005.689.59.

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4

Levenko, B. A., and M. A. Rubtsova. "HERBICIDE RESISTANT TRANSGENIC PLANTS OF GRAPEVINE." Acta Horticulturae, no. 528 (May 2000): 339–42. http://dx.doi.org/10.17660/actahortic.2000.528.46.

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5

Gray, D. J., Z. T. Li, D. L. Hopkins, et al. "Transgenic Grapevines Resistant to Pierce's Disease." HortScience 40, no. 4 (2005): 1104D—1105. http://dx.doi.org/10.21273/hortsci.40.4.1104d.

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Pierce's disease (PD), caused by the xylem-limited bacterium Xylella fastidiosa, is endemic to the coastal plain of the southeastern United States. Although native southern grapevines are tolerant to X. fastidiosa, all varieties of Vitisvinifera grown in the region will succumb to PD. Genetic transformation to add disease resistance genes, while not disturbing desirable phenotypic characters, holds promise for expanding the southeastern U.S. grape industry by allowing use of established fruit and wine varieties. We utilize embryogenic cell cultures and Agrobacterium strain EHA105 to refine tra

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6

Krastanova, S., K. S. Ling, H. Y. Zhu, B. Xue, T. J. Burr, and D. Gonsalves. "DEVELOPMENT OF TRANSGENIC GRAPEVINE ROOTSTOCKS WITH GENES FROM GRAPEVINE FANLEAF VIRUS AND GRAPEVINE LEAFROLL ASSOCIATED CLOSTEROVIRUSES 2 AND 3." Acta Horticulturae, no. 528 (May 2000): 367–72. http://dx.doi.org/10.17660/actahortic.2000.528.52.

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7

Dutt, Manjul, Dennis J. Gray, Zhijian T. Li, Sadanand Dhekney, and Marilyn M. Van Aman. "Micropropagation Cultures for Genetic Transformation of Grapevine." HortScience 41, no. 4 (2006): 972C—972. http://dx.doi.org/10.21273/hortsci.41.4.972c.

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A major drawback to the use of embryogenic cultures for transformation of grapevine is that their ability to undergo genetic transformation is cultivar-dependent. Also, depending on cultivar, embryogenic cultures are difficult to impossible to maintain over time, reducing their utility for use in genetic transformation. An alternative to the use of embryogenic cultures for transformation of grapevine is the use of micropropagation cultures, which are easier to initiate from a wide range of grapevine cultivars and can be maintained over time without loss of function. Vitis vinifera `Thompson Se

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8

Aleynova, Olga A., Konstantin V. Kiselev, Zlata V. Ogneva, and Alexandra S. Dubrovina. "The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response." International Journal of Molecular Sciences 21, no. 21 (2020): 7939. http://dx.doi.org/10.3390/ijms21217939.

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Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded proteins with distinct N-terminal regions. We studied the transcript abundance of CML21v1, CML21v2, CML21v3, and CML21v4 in wild-growing grapevine Vitis amurensis Rupr. in response to desiccation, heat, cold, high salinity, and high mannitol stress using quantitative real-time RT-PCR. The levels of

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9

Li, Wei, Changxi Dang, Yuxiu Ye, et al. "Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco." International Journal of Molecular Sciences 21, no. 4 (2020): 1323. http://dx.doi.org/10.3390/ijms21041323.

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In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators that regulate developmental process and responses to phytohormones and stress treatments. However, the regulatory functions of the Vitis vinifera L. (grapevine) Aux/IAA transcription factor gene VvIAA18 have not been reported. In this study, the VvIAA18 gene was successfully cloned from grapevine. Subcellular localization analysis in onion epidermal cells indicated that VvIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length of VvIAA18 exhibited transcriptional activation

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10

Rubtsova, M. A., and B. A. Levenko. "PHOSPHINOTHRICIN- AND CROWN GALL-RESISTANT TRANSGENIC PLANTS OF GRAPEVINE." Acta Horticulturae, no. 625 (September 2003): 465–72. http://dx.doi.org/10.17660/actahortic.2003.625.55.

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11

Zok, A., I. Forgács, A. Pedryc, R. Oláh, and E. Szegedi. "Agrobacterium tumefaciens virE1Inhibits crown gall development in transgenic grapevine." Acta Alimentaria 41, Supplement 1 (2012): 214–18. http://dx.doi.org/10.1556/aalim.41.2012.suppl.21.

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12

Voegel, Tanja M., Jeremy G. Warren, Ayumi Matsumoto, Michele M. Igo, and Bruce C. Kirkpatrick. "Localization and characterization of Xylella fastidiosa haemagglutinin adhesins." Microbiology 156, no. 7 (2010): 2172–79. http://dx.doi.org/10.1099/mic.0.037564-0.

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Xylella fastidiosa is a Gram-negative, xylem-inhabiting, plant-pathogenic bacterium responsible for several important diseases including Pierce's disease (PD) of grapevines. The bacteria form biofilms in grapevine xylem that contribute to the occlusion of the xylem vessels. X. fastidiosa haemagglutinin (HA) proteins are large afimbrial adhesins that have been shown to be crucial for biofilm formation. Little is known about the mechanism of X. fastidiosa HA-mediated cell–cell aggregation or the localization of the adhesins on the cell. We generated anti-HA antibodies and show that X. fastidiosa

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13

Ju, Yan-lun, Zhuo Min, Xiao-feng Yue, et al. "Overexpression of grapevine VvNAC08 enhances drought tolerance in transgenic Arabidopsis." Plant Physiology and Biochemistry 151 (June 2020): 214–22. http://dx.doi.org/10.1016/j.plaphy.2020.03.028.

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14

Boss, Paul K., Lekha Sreekantan, and Mark R. Thomas. "A grapevine TFL1 homologue can delay flowering and alter floral development when overexpressed in heterologous species." Functional Plant Biology 33, no. 1 (2006): 31. http://dx.doi.org/10.1071/fp05191.

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Grapevines (Vitis vinifera L.) have unusual plant architecture in that the shoot apical meristem produces both vegetative structures and primordia that are capable of forming inflorescences at regular intervals. These primordia are termed ‘uncommitted’ and differentiate into inflorescences or tendrils depending on the environment in which they are produced. To investigate the molecular relationship between tendrils and inflorescences and vine architecture, we cloned a TFL1 homologue from grapevine (VvTFL1). VvTFL1 is expressed in shoot apices early in latent bud development and in buds soon af

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15

Vigne, Emmanuelle, Véronique Komar, and Marc Fuchs. "Field Safety Assessment of Recombination in Transgenic Grapevines Expressing the Coat Protein Gene of Grapevine fanleaf virus." Transgenic Research 13, no. 2 (2004): 165–79. http://dx.doi.org/10.1023/b:trag.0000026075.79097.c9.

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16

Vandelle, Elodie, Pietro Ariani, Alice Regaiolo, et al. "The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen Plasmopara viticola." International Journal of Molecular Sciences 22, no. 2 (2021): 940. http://dx.doi.org/10.3390/ijms22020940.

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Downy mildew, caused by Plasmopara viticola, is one of the most severe diseases of grapevine (Vitis vinifera L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors) introgressed from wild Vitis species, although effective, may be non-durable because the pathogen can evolve to avoid specific recognition. Previous transcriptomic studies in the resistant species Vitis riparia highlighted the activation of signal transduction components during infection. The transfer of such components to V. vinifera

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17

Dalla Costa, Lorenza, Daniela Vinciguerra, Lisa Giacomelli, et al. "Integrated approach for the molecular characterization of edited plants obtained via Agrobacterium tumefaciens-mediated gene transfer." European Food Research and Technology 248, no. 1 (2021): 289–99. http://dx.doi.org/10.1007/s00217-021-03881-0.

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AbstractAgrobacterium tumefaciens-mediated gene transfer—actually the most used method to engineer plants—may lead to integration of multiple copies of T-DNA in the plant genome, as well as to chimeric tissues composed of modified cells and wild type cells. A molecular characterization of the transformed lines is thus a good practice to select the best ones for further investigation. Nowadays, several quantitative and semi-quantitative techniques are available to estimate the copy number (CN) of the T-DNA in genetically modified plants. In this study, we compared three methods based on (1) rea

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18

Aguero, C. B., A. M. Dandekar, and C. P. Meredith. "TRANSGENIC GRAPEVINE PLANTS EXPRESSING GREEN FLUORESCENT PROTEINS TARGETED TO THE APOPLAST." Acta Horticulturae, no. 689 (August 2005): 475–780. http://dx.doi.org/10.17660/actahortic.2005.689.57.

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19

Li, Peiying, Dongdong Yu, Bao Gu, Hongjuan Zhang, Qiying Liu, and Jianxia Zhang. "Overexpression of the VaERD15 gene increases cold tolerance in transgenic grapevine." Scientia Horticulturae 293 (February 2022): 110728. http://dx.doi.org/10.1016/j.scienta.2021.110728.

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20

Burger, Anita L., Leonora Watts, and Frederik C. Botha. "Grapevine promoter directs gene expression in the nectaries of transgenic tobacco." Physiologia Plantarum 126, no. 3 (2006): 418–34. http://dx.doi.org/10.1111/j.1399-3054.2006.00598.x.

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21

Harst, Margit, Beatrix-Axinja Cobanov, Ludger Hausmann, Rudolf Eibach, and Reinhard Töpfer. "Evaluation of pollen dispersal and cross pollination using transgenic grapevine plants." Environmental Biosafety Research 8, no. 2 (2009): 87–99. http://dx.doi.org/10.1051/ebr/2009012.

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22

Li, Hui, Zhen Gao, Qiuju Chen, et al. "Grapevine ABA receptor VvPYL1 regulates root hair development in Transgenic Arabidopsis." Plant Physiology and Biochemistry 149 (April 2020): 190–200. http://dx.doi.org/10.1016/j.plaphy.2020.02.008.

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23

Jiu, SongTao, Chen Wang, Ting Zheng, et al. "Characterization of VvPAL-like promoter from grapevine using transgenic tobacco plants." Functional & Integrative Genomics 16, no. 6 (2016): 595–617. http://dx.doi.org/10.1007/s10142-016-0516-x.

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24

Vigne, Emmanuelle, Marc Bergdoll, Sébastien Guyader, and Marc Fuchs. "Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination." Journal of General Virology 85, no. 8 (2004): 2435–45. http://dx.doi.org/10.1099/vir.0.79904-0.

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The nematode-borne Grapevine fanleaf virus, from the genus Nepovirus in the family Comoviridae, causes severe degeneration of grapevines in most vineyards worldwide. We characterized 347 isolates from transgenic and conventional grapevines from two vineyard sites in the Champagne region of France for their molecular variant composition. The population structure and genetic diversity were examined in the coat protein gene by IC-RT-PCR-RFLP analysis with EcoRI and StyI, and nucleotide sequencing, respectively. RFLP data suggested that 55 % (191 of 347) of the isolates had a population structure

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25

Li, Min, Si-qi Shen, Yi-bin Xing, et al. "Vitis vinifera VvPUB17 functions as a E3 ubiquitin ligase and enhances powdery mildew resistance via the salicylic acid signaling pathway." Journal of Berry Research 11, no. 3 (2021): 419–30. http://dx.doi.org/10.3233/jbr-210709.

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BACKGROUND: Powdery mildew affects grapevine growth and development and reduces grapevine fruit yield and quality. Plant U-box (PUB) E3 ubiquitin ligases play important roles in ubiquitin/proteasome-mediated protein degradation during plant development and in the plant defense response. OBJECTIVE: We cloned the VvPUB17 gene from Vitis vinifera and analyzed that VvPUB17 enhanced the resistance of grapevine to powdery mildew through the SA signal pathway. METHODS: Pathogen inoculation of Arabidopsis thaliana and grapevine plants was carried out by the tableting method. Gene expression was analyz

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26

Arrey-Salas, Oscar, José Carlos Caris-Maldonado, Bairon Hernández-Rojas, and Enrique Gonzalez. "Comprehensive Genome-Wide Exploration of C2H2 Zinc Finger Family in Grapevine (Vitis vinifera L.): Insights into the Roles in the Pollen Development Regulation." Genes 12, no. 2 (2021): 302. http://dx.doi.org/10.3390/genes12020302.

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Some C2H2 zinc-finger proteins (ZFP) transcription factors are involved in the development of pollen in plants. In grapevine (Vitis vinifera L.), it has been suggested that abnormalities in pollen development lead to the phenomenon called parthenocarpy that occurs in some varieties of this cultivar. At present, a network involving several transcription factors types has been revealed and key roles have been assigned to members of the C2H2 zinc-finger proteins (ZFP) family in model plants. However, particularities of the regulatory mechanisms controlling pollen formation in grapevine remain unk

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27

Monier, C., P. Barbier, and B. Walter. "PROTECTION AGAINST GRAPEVINE FANLEAF VIRUS IN TRANSGENIC TOBACCO CONTAINING NON-TRANSLATABLE SEQUENCES." Acta Horticulturae, no. 528 (May 2000): 379–84. http://dx.doi.org/10.17660/actahortic.2000.528.54.

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28

Zok, A., R. Oláh, É. Hideg, et al. "Effect of Medicago sativa ferritin gene on stress tolerance in transgenic grapevine." Plant Cell, Tissue and Organ Culture (PCTOC) 100, no. 3 (2009): 339–44. http://dx.doi.org/10.1007/s11240-009-9641-8.

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29

Martinelli, L., and G. Mandolino. "Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S.)." Theoretical and Applied Genetics 88, no. 6-7 (1994): 621–28. http://dx.doi.org/10.1007/bf01253963.

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30

Hanif, Muhammad, Mati Rahman, Min Gao, et al. "Heterologous Expression of the Grapevine JAZ7 Gene in Arabidopsis Confers Enhanced Resistance to Powdery Mildew but Not to Botrytis cinerea." International Journal of Molecular Sciences 19, no. 12 (2018): 3889. http://dx.doi.org/10.3390/ijms19123889.

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Jasmonate ZIM-domain (JAZ) family proteins comprise a class of transcriptional repressors that silence jasmonate-inducible genes. Although a considerable amount of research has been carried out on this gene family, there is still very little information available on the role of specific JAZ gene members in multiple pathogen resistance, especially in non-model species. In this study, we investigated the potential resistance function of the VqJAZ7 gene from a disease-resistant wild grapevine, Vitis quinquangularis cv. “Shang-24”, through heterologous expression in Arabidopsis thaliana. VqJAZ7-ex

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31

Laquitaine, Laurent, Eric Gomès, Julie François, et al. "Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression." Molecular Plant-Microbe Interactions® 19, no. 10 (2006): 1103–12. http://dx.doi.org/10.1094/mpmi-19-1103.

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Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-gl

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32

Zhu, Ziguo, Guirong Li, Chaohui Yan, et al. "DRL1, Encoding A NAC Transcription Factor, Is Involved in Leaf Senescence in Grapevine." International Journal of Molecular Sciences 20, no. 11 (2019): 2678. http://dx.doi.org/10.3390/ijms20112678.

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The NAC (for NAM, ATAF1,2, and CUC2) proteins family are plant-specific transcription factors, which play important roles in leaf development and response to environmental stresses. In this study, an NAC gene, DRL1, isolated from grapevine Vitis vinifera L. “Yatomi Rose”, was shown to be involved in leaf senescence. The quantity of DRL1 transcripts decreased with advancing leaf senescence in grapevine. Overexpressing the DRL1 gene in tobacco plants significantly delayed leaf senescence with respect to chlorophyll concentration, potential quantum efficiency of photosystem II (Fv/Fm), and ion le

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33

Nakano, M., Y. Hoshino, and M. Mii. "Regeneration of transgenic plants of grapevine (Vitis viniferaL.) viaAgrobacteriumrhizogenesmediated transformation of embryogenic calli." Journal of Experimental Botany 45, no. 5 (1994): 649–56. http://dx.doi.org/10.1093/jxb/45.5.649.

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34

Yamamoto, T., H. Iketani, H. Ieki, et al. "Transgenic grapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogens." Plant Cell Reports 19, no. 7 (2000): 639–46. http://dx.doi.org/10.1007/s002999900174.

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35

Galambos, A., A. Zok, A. Kuczmog, et al. "Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance." Plant Cell Reports 32, no. 11 (2013): 1751–57. http://dx.doi.org/10.1007/s00299-013-1488-0.

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36

Zhu, Ziguo, Guirong Li, Li Liu, et al. "A R2R3-MYB Transcription Factor, VvMYBC2L2, Functions as a Transcriptional Repressor of Anthocyanin Biosynthesis in Grapevine (Vitis vinifera L.)." Molecules 24, no. 1 (2018): 92. http://dx.doi.org/10.3390/molecules24010092.

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In grapevine, the MYB transcription factors play an important role in the flavonoid pathway. Here, a R2R3-MYB transcription factor, VvMYBC2L2, isolated from Vitis vinifera cultivar Yatomi Rose, may be involved in anthocyanin biosynthesis as a transcriptional repressor. VvMYBC2L2 was shown to be a nuclear protein. The gene was shown to be strongly expressed in root, flower and seed tissue, but weakly expressed during the fruit development in grapevine. Overexpressing the VvMYBC2L2 gene in tobacco resulted in a very marked decrease in petal anthocyanin concentration. Expression analysis of flavo

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37

Takuhara, Yuki, Masayuki Kobayashi, and Shunji Suzuki. "Low-temperature-induced transcription factors in grapevine enhance cold tolerance in transgenic Arabidopsis plants." Journal of Plant Physiology 168, no. 9 (2011): 967–75. http://dx.doi.org/10.1016/j.jplph.2010.11.008.

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38

Krastanova, Stoyanka V., Vasudevan Balaji, Michele R. Holden, et al. "Resistance to crown gall disease in transgenic grapevine rootstocks containing truncated virE2 of Agrobacterium." Transgenic Research 19, no. 6 (2010): 949–58. http://dx.doi.org/10.1007/s11248-010-9373-x.

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39

Thomzik, J. E., K. Stenzel, R. Stöcker, P. H. Schreier, R. Hain, and D. J. Stahl. "Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentumMill.) conditions resistance againstPhytophthora infestans." Physiological and Molecular Plant Pathology 51, no. 4 (1997): 265–78. http://dx.doi.org/10.1006/pmpp.1997.0123.

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40

Kikkert, Julie R., Dominique Hébert-Soulé, Patricia G. Wallace, Michael J. Striem, and Bruce I. Reisch. "Transgenic plantlets of ‘Chancellor’ grapevine (Vitis sp.) from biolistic transformation of embryogenic cell suspensions." Plant Cell Reports 15, no. 5 (1996): 311–16. http://dx.doi.org/10.1007/bf00232362.

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41

Grimmig, Bernhard, Roland Schubert, Regina Fischer, et al. "Ozone- and ethylene-induced regulation of a grapevine resveratrol synthase promoter in transgenic tobacco." Acta Physiologiae Plantarum 19, no. 4 (1997): 467–74. http://dx.doi.org/10.1007/s11738-997-0043-4.

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42

Kikkert, Julie R., Dominique H�bert-Soul�, Patricia G. Wallace, Michael J. Striem, and Bruce I. Reisch. "Transgenic plantlets of 'Chancellor' grapevine ( Vitis sp.) from biolistic transformation of embryogenic cell suspensions." Plant Cell Reports 15, no. 5 (1996): 311–16. http://dx.doi.org/10.1007/s002990050023.

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43

Cheng, Jing, Keji Yu, Mingyue Zhang, Ying Shi, Changqing Duan, and Jun Wang. "The Effect of Light Intensity on the Expression of Leucoanthocyanidin Reductase in Grapevine Calluses and Analysis of Its Promoter Activity." Genes 11, no. 10 (2020): 1156. http://dx.doi.org/10.3390/genes11101156.

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To investigate the effect of light intensity on flavonoid biosynthesis, grapevine calluses were subjected to high light (HL, 250 μmol m−2 s−1) and dark (0 μmol m−2 s−1) in comparison to 125 μmol m−2 s−1 under controlled conditions (NL). The alteration of flavonoid profiles was determined and was integrated with RNA sequencing (RNA-seq)-based transcriptional changes of the flavonoid pathway genes. Results revealed that dark conditions inhibited flavonoid biosynthesis. Increasing light intensity affected flavonoids differently—the concentrations of flavonols and anthocyanins as well as the expre

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44

Le Gall, O., L. Torregrosa, Y. Danglot, T. Candresse, and A. Bouquet. "Agrobacterium-mediated genetic transformation of grapevine somatic embryos and regeneration of transgenic plants expressing the coat protein of grapevine chrome mosaic nepovirus (GCMV)." Plant Science 102, no. 2 (1994): 161–70. http://dx.doi.org/10.1016/0168-9452(94)90034-5.

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45

Yoshikawa, N., S. Gotoh, M. Umezawa, et al. "Transgenic Nicotiana occidentalis Plants Expressing the 50-kDa Protein of Apple chlorotic leaf spot virus Display Increased Susceptibility to Homologous Virus, but Strong Resistance to Grapevine berry inner necrosis virus." Phytopathology® 90, no. 3 (2000): 311–16. http://dx.doi.org/10.1094/phyto.2000.90.3.311.

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The 50-kDa protein (P50) encoded by the open reading frame 2 of Apple chlorotic leaf spot virus (ACLSV), a putative movement protein, was expressed in transgenic Nicotiana occidentalis plants. P50 in transgenic plants was mainly detected in a modified form in the cell wall fraction, similar to that in infected leaves. The P50-expressing plants (P50 plants) complemented the systemic spread of the P50-defective mutants of an infectious cDNA clone of ACLSV (pCLSF), indicating that P50 in transgenic plants was functional. Severity of symptoms was greatly enhanced and accumulation of virus in upper

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46

Zhang, Zhan, Luming Zou, Chong Ren, et al. "VvSWEET10 Mediates Sugar Accumulation in Grapes." Genes 10, no. 4 (2019): 255. http://dx.doi.org/10.3390/genes10040255.

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Sugar accumulation is a critical event during grape berry ripening that determines the grape market values. Berry cells are highly dependent on sugar transporters to mediate cross-membrane transport. However, the role of sugar transporters in improving sugar accumulation in berries is not well established in grapes. Herein we report that a Sugars Will Eventually be Exported Transporter (SWEET), that is, VvSWEET10, was strongly expressed at the onset of ripening (véraison) and can improve grape sugar content. VvSWEET10 encodes a plasma membrane-localized transporter, and the heterologous expres

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47

Hily, Jean-Michel, Sandrine Demanèche, Nils Poulicard, et al. "Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome." Plant Biotechnology Journal 16, no. 1 (2017): 208–20. http://dx.doi.org/10.1111/pbi.12761.

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Yoshikawa, N., Y. Saitou, A. Kitajima, T. Chida, N. Sasaki, and M. Isogai. "Interference of Long-Distance Movement of Grapevine berry inner necrosis virus in Transgenic Plants Expressing a Defective Movement Protein of Apple chlorotic leaf spot virus." Phytopathology® 96, no. 4 (2006): 378–85. http://dx.doi.org/10.1094/phyto-96-0378.

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Transgenic Nicotiana occidentalis plants expressing a movement protein (P50) and partially functional deletion mutants (ΔA and ΔC) of the Apple chlorotic leaf spot virus (ACLSV) showed resistance to Grapevine berry inner necrosis virus (GINV). The resistance is highly effective and GINV was below the level of detection in both inoculated and uninoculated upper leaves. In contrast, GINV accumulated in inoculated and uninoculated leaves of nontransgenic (NT) plants and transgenic plants expressing a dysfunctional mutant (ΔG). On the other hand, in some plants of a transgenic plant line expressin

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Ritzenthaler, C., C. Laporte, F. Gaire, et al. "Grapevine Fanleaf Virus Replication Occurs on Endoplasmic Reticulum-Derived Membranes." Journal of Virology 76, no. 17 (2002): 8808–19. http://dx.doi.org/10.1128/jvi.76.17.8808-8819.2002.

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ABSTRACT Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic modifications of the host endomembrane system that eventually culminate in the formation of a perinuclear “viral compartment.” We identified by immunoconfocal microscopy this compartment as the site of virus replication since it contained the RNA1-encoded proteins necessary for replication, newly synthesized viral RNA, and double-stranded replicative forms. In addition, by using transgenic T-BY2 protoplasts expressing green fluore

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Valat, Laure, Marc Fuchs, and Monique Burrus. "Transgenic grapevine rootstock clones expressing the coat protein or movement protein genes of Grapevine fanleaf virus: Characterization and reaction to virus infection upon protoplast electroporation." Plant Science 170, no. 4 (2006): 739–47. http://dx.doi.org/10.1016/j.plantsci.2005.11.005.

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