Academic literature on the topic 'Translocation direction'

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Journal articles on the topic "Translocation direction"

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ANASTASI, ANGELA, CHERRYL HUNT, and HOWARD STEBBINGS. "Isolation of microtubule motors from an insect ovarian system: characterization using a novel motility substratum." Journal of Cell Science 96, no. 1 (1990): 63–69. http://dx.doi.org/10.1242/jcs.96.1.63.

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The ovaries of hemipteran insects contain massive microtubule-based translocation channels known as nutritive tubes, linking nurse cells to the developing oocytes. Translocation, which is in a retrograde direction along the nutritive tube microtubules, has previously been reactivated in vitro. Here, ATPsensitive microtubule-associated proteins (MAPs) have been isolated from the insect ovaries, and beads coated with such proteins applied to salt-treated, detergent-extracted nutritive tube microtubules microdissected from the insect ovaries. These motility substrata are composed of many thousands of parallel microtubules, all with a common known polarity, so that not only are they easily observed, but the direction of any translocation along their length can be readily interpreted. ATP extracts of insect ovarian MAPs, containing both kinesin and dynein, were seen to promote bidirectional movements of beads. Movements in the two directions differed in both rate and form. On fractionation of the ATP extract, those fractions containing kinesin brought about bead movement in an anterograde direction. Fractions containing dynein failed to promote movement of beads, and no single fraction promoted movement of beads in a retrograde direction. Kinesin, while clearly present in the insect ovary, is absent from the nutritive tube translocation channels. The nutritive tubes, however, contain a polypeptide that co-electrophoreses with insect ovarian dynein, making dynein a possible candidate for the motor that drives the retrograde translocation along nutritive tubes.
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Olsnes, Sjur, Jan Øivind Moskaug, Harald Stenmark, and Kirsten Sandvig. "Diphtheria toxin entry: protein translocation in the reverse direction." Trends in Biochemical Sciences 13, no. 9 (1988): 348–51. http://dx.doi.org/10.1016/0968-0004(88)90105-3.

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Petr, Novák, and Hůla Josef. "Translocation of the upper soil layer in multiple operations of seedbed preparation." Research in Agricultural Engineering 63, Special Issue (2017): S46—S52. http://dx.doi.org/10.17221/40/2017-rae.

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Translocation of tracers incorporated into the upper layer of topsoil was evaluated in the course of seedbed preparation for winter wheat. Aluminium cubes with the edge length of 16 mm were used as tracers that were placed into the soil before its tillage into furrows perpendicular to the direction of passes. After the passes of the OPALL-AGRI combined cultivator, the tracers were searched and marked using a metal detector. The translocation of tracers was evaluated during multiple passes on flatland and on the slope. During the seedbed preparation on the slope, downslope and upslope passes in a fall line direction were chosen. Results of the tracers movement measuring that simulate the soil particle translocation indicate a pronounced movement of the upper layer of topsoil during multiple downslope passes of the combined cultivator. A significant translocation was also observed after passes on flatland. A significantly smaller lengthwise translocation of tracers was found out at upslope passes. The type of translocation on flatland and upslope was quite similar, on the other hand, downslope movement was much larger. The upslope passes were found to have a very limited function with regard to the correction of the undesirable downslope movement of soil particles that occurs in the course of tillage.
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Fasullo, M. T., and R. W. Davis. "Direction of chromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational substrates." Molecular and Cellular Biology 8, no. 10 (1988): 4370–80. http://dx.doi.org/10.1128/mcb.8.10.4370.

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We used the his3 recombinational substrates (his3 fragments) to direct large interchromosomal (translocations) and intrachromosomal (deletions and tandem duplications) rearrangements in the yeast Saccharomyces cerevisiae. In strains completely deleted for the wild-type HIS3 gene, his3 fragments, one containing a deletion of 5' amino acid coding sequences and the other containing a deletion of 3' amino acid coding sequences, were first placed at preselected sites by homologous recombination. His+ revertants that arose via spontaneous mitotic recombination between the two his3 fragments were selected. This strategy was used to direct rearrangements in both RAD52+ and rad52 mutant strains. Translocations occurred in the RAD52+ genetic background and were characterized by orthogonal field alternating gel electrophoresis of yeast chromosomal DNA and by standard genetic techniques. An unexpected translocation was also identified in which HIS3 sequences were amplified. Two types of tandem duplications of the GAL(7, 10, 1) locus were also directed, and one type was not observed in rad52 mutants. Recombination mechanisms are discussed to account for these differences.
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Fasullo, M. T., and R. W. Davis. "Direction of chromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational substrates." Molecular and Cellular Biology 8, no. 10 (1988): 4370–80. http://dx.doi.org/10.1128/mcb.8.10.4370-4380.1988.

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We used the his3 recombinational substrates (his3 fragments) to direct large interchromosomal (translocations) and intrachromosomal (deletions and tandem duplications) rearrangements in the yeast Saccharomyces cerevisiae. In strains completely deleted for the wild-type HIS3 gene, his3 fragments, one containing a deletion of 5' amino acid coding sequences and the other containing a deletion of 3' amino acid coding sequences, were first placed at preselected sites by homologous recombination. His+ revertants that arose via spontaneous mitotic recombination between the two his3 fragments were selected. This strategy was used to direct rearrangements in both RAD52+ and rad52 mutant strains. Translocations occurred in the RAD52+ genetic background and were characterized by orthogonal field alternating gel electrophoresis of yeast chromosomal DNA and by standard genetic techniques. An unexpected translocation was also identified in which HIS3 sequences were amplified. Two types of tandem duplications of the GAL(7, 10, 1) locus were also directed, and one type was not observed in rad52 mutants. Recombination mechanisms are discussed to account for these differences.
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Buyukdagli, Sahin. "Facilitated polymer capture by charge inverted electroosmotic flow in voltage-driven polymer translocation." Soft Matter 14, no. 18 (2018): 3541–49. http://dx.doi.org/10.1039/c8sm00620b.

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The optimal functioning of nanopore-based sequencing tools necessitates rapid polymer capture by anionic membrane pores. This goal can be achieved by polyvalent cation addition that inverts the direction of the electroosmotic flow. The inverted flow drags the polymer in the trans direction and speeds up its capture by the nanopore.
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Elezovic, Ibrahim, Dragana Bozic, and Sava Vrbnicanin. "Absorption, translocation and metabolism of the sulfonylurea herbicides in plants." Pesticidi 18, no. 4 (2003): 205–22. http://dx.doi.org/10.2298/pif0304205e.

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Absorption, translocation and metabolism are processes affecting the efficacy of sulfonylurea herbicides. These processes contribute significantly to selectivity but are also known to effect the development of plant resistance to this group of herbicides. Sulfonylureas may be absorbed by both ground and above ground plant parts. The level of absorption depends on numerous factors such as: development stage of the plant, ambient temperature, soil humidity, fertilizers added to herbicides, application in combination with other herbicides, sufactants, plant cultivars, mode of herbicide application, various additives. Having been absorbed, the herbicide moves to the place of action whereby the direction of translocation depends on the mode of absorption. Foliary absorbed sulfonylureas are primarily basipetally translocated. Acropetal translocation is correlated to root absorption. In addition, some herbicides belonging to this group are translocated in both directions. The level and rate of translocation depend on: soil humidity, antagonistic effect of other herbicides (in case of application of herbicide combinations), additives, fertilizers etc. Sulfonylureas in plants are subjected to different metabolic changes which mostly contribute to the inactivation. These transformations are considered to be catalyzed by the cytochrom P-450 monooxigenase enzymic system. Eventually, this process is also known to be affected by numerous factors such as: temperature, soil humidity, plant cultivars, mixtures with other pesticides etc.
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Xu, H. C., L. Z. Jia, J. H. Zhang, Z. H. Zhang, and Y. H. Wei. "Combined effects of tillage direction and slope gradient on soil translocation by hoeing." CATENA 175 (April 2019): 421–29. http://dx.doi.org/10.1016/j.catena.2018.12.039.

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Olivares, Adrian O., Hema Chandra Kotamarthi, Benjamin J. Stein, Robert T. Sauer, and Tania A. Baker. "Effect of directional pulling on mechanical protein degradation by ATP-dependent proteolytic machines." Proceedings of the National Academy of Sciences 114, no. 31 (2017): E6306—E6313. http://dx.doi.org/10.1073/pnas.1707794114.

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AAA+ proteases and remodeling machines couple hydrolysis of ATP to mechanical unfolding and translocation of proteins following recognition of sequence tags called degrons. Here, we use single-molecule optical trapping to determine the mechanochemistry of two AAA+ proteases, Escherichia coli ClpXP and ClpAP, as they unfold and translocate substrates containing multiple copies of the titinI27 domain during degradation initiated from the N terminus. Previous studies characterized degradation of related substrates with C-terminal degrons. We find that ClpXP and ClpAP unfold the wild-type titinI27 domain and a destabilized variant far more rapidly when pulling from the N terminus, whereas translocation speed is reduced only modestly in the N-to-C direction. These measurements establish the role of directionality in mechanical protein degradation, show that degron placement can change whether unfolding or translocation is rate limiting, and establish that one or a few power strokes are sufficient to unfold some protein domains.
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Mazauric, Marie-Hélène, Yeonee Seol, Satoko Yoshizawa, Koen Visscher, and Dominique Fourmy. "Interaction of the HIV-1 frameshift signal with the ribosome." Nucleic Acids Research 37, no. 22 (2009): 7654–64. http://dx.doi.org/10.1093/nar/gkp779.

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Abstract Ribosomal frameshifting on viral RNAs relies on the mechanical properties of structural elements, often pseudoknots and more rarely stem-loops, that are unfolded by the ribosome during translation. In human immunodeficiency virus (HIV)-1 type B a long hairpin containing a three-nucleotide bulge is responsible for efficient frameshifting. This three-nucleotide bulge separates the hairpin in two domains: an unstable lower stem followed by a GC-rich upper stem. Toeprinting and chemical probing assays suggest that a hairpin-like structure is retained when ribosomes, initially bound at the slippery sequence, were allowed multiple EF-G catalyzed translocation cycles. However, while the upper stem remains intact the lower stem readily melts. After the first, and single step of translocation of deacylated tRNA to the 30 S P site, movement of the mRNA stem-loop in the 5′ direction is halted, which is consistent with the notion that the downstream secondary structure resists unfolding. Mechanical stretching of the hairpin using optical tweezers only allows clear identification of unfolding of the upper stem at a force of 12.8 ± 1.0 pN. This suggests that the lower stem is unstable and may indeed readily unfold in the presence of a translocating ribosome.
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Dissertations / Theses on the topic "Translocation direction"

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Galoin, Sandrine. "Contribution à l'étude de la translocation t(14 ; 18) dans les lymphomes folliculaires : intérêt des sondes clonospécifiques dans la direction de la maladie résiduelle." Toulouse 3, 2001. http://www.theses.fr/2001TOU30140.

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Steinbock, L. J., S. Krishnan, R. D. Bulushev, et al. "Probing the size of proteins with glass nanopores." Royal Society of Chemistry, 2014. https://tud.qucosa.de/id/qucosa%3A36309.

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Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensitivity was enhanced by electron reshaping which decreased the nanopore diameter and created geometries with a reduced sensing length. Further, proteins having molecular weights (MW) ranging from 12 kDa to 480 kDa were detected, which showed that their corresponding current peak amplitude changes according to their MW. In the case of the 12 kDa ComEA protein, its DNA-binding properties to an 800 bp long DNA molecule was investigated. Moreover, the influence of the pH on the charge of the protein was demonstrated by showing a change in the translocation direction. This work emphasizes the wide spectrum of detectable molecules using nanopores from glass nanocapillaries, which stand out because of their inexpensive, lithography-free, and rapid manufacturing process
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Besprozvannaya, Marina. "From DNA sequence recognition to directional chromosome segregation: Information transfer in the translocase protein SpoIIIE." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11373.

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Faithful chromosome segregation is essential for all living organisms. Bacterial chromosome segregation utilizes highly conserved directional SpoIIIE/FtsK translocases to move large DNA molecules between spatially separated compartments. These translocases employ an accessory DNA-interacting domain (gamma) that dictates the direction of DNA transport by recognizing specific DNA sequences. To date it remains unclear how these translocases use DNA sequence information as a trigger to expend chemical energy (ATP turnover) and thereby power mechanical work (DNA movement). In this thesis, I undertook a mechanistic study of directional DNA movement by SpoIIIE from the Gram-positive model bacterium Bacillus subtilis. Specifically, I was interested in understanding the information transfer within the protein from sequence recognition, to ATP turnover, and ultimately to chromosome translocation. How do DNA sequences trigger directional chromosome movement?
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Books on the topic "Translocation direction"

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Armstrong, Doug, Matthew Hayward, Dorian Moro, and Philip Seddon, eds. Advances in Reintroduction Biology of Australian and New Zealand Fauna. CSIRO Publishing, 2015. http://dx.doi.org/10.1071/9781486303021.

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The publication of Reintroduction Biology of Australian and New Zealand Fauna nearly 20 years ago introduced the new science of ‘reintroduction biology’. Since then, there have been vast changes in our understanding of the process of reintroductions and other conservation-driven translocations, and corresponding changes in regulatory frameworks governing translocations. 
 
 Advances in Reintroduction Biology of Australian and New Zealand Fauna is a timely review of our understanding of translocation from an Australasian perspective, ensuring translocation becomes an increasingly effective conservation management strategy in the future. Written by experts, including reintroduction practitioners, researchers and policy makers, the book includes extensive practical advice and example case studies, identifies emerging themes and suggests future directions. 
 
 Conservation practitioners and researchers, as well as conservation management agencies and NGOs will find the book a valuable resource. Although it is based on Australasian examples, it will be of interest globally due to synergies with reintroduction programs throughout the world.
 
 2015 Whitley Awards Certificate of Commendation for Conservation Biology.
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Conference papers on the topic "Translocation direction"

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HŮLA, Josef, and Petr NOVÁK. "IMPACT OF WORKING SPEED ON SOIL PARTICLES TRANSFER DURING SECONDARY SOIL TILLAGE." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.206.

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Displacement of soil particles by erosion can be seen as a major threat to the quality of agricultural land in the conditions of Czech Republic. While the effects of water and wind erosion have long been investigated and reported, the effect of soil tillage technology on soil particles translocation are relatively new area of agriculture research. Soil tillage may contribute to the undesirable translocation of soil particles towards lower-lying parts of fields especially on slopes. The effect of soil tillage implements on soil particle translocation has not been sufficiently explained yet. The object of this research was to assess the influence of different operating speed of power harrow on soil particle translocation during secondary tillage (soil preparation). Measurements to determine the displacement of soil particles were performed in location Nesperská Lhota in the Central Bohemia Region. Measurements were performed on a sandy loam cambisol after harvest spring cereals (oat for sillage production). To indicate displacement of soil particles was used grit of white limestone (size 10-16 mm). Limestone was put down into the trench with known position orthogonal to the direction of working operations. Subsequently were performed working operations in the specified sequence. Limestone particles were counted and weighed in each section. It was detected by measuring the different nature of displacement. Statistical significance of differences in the weight of translocated particles was evaluated for different operating speeds of power harrow from 4.5 to 12 km.h-1.
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Li, Kun, Wei Si, Jingjie Sha, and Yunfei Chen. "Molecular Dynamics Study of DNA Translocation Through Graphene Nanopores With Controllable Speed." In ASME 2015 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/imece2015-50858.

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All-atom steered molecular dynamics (SMD) simulations provide the means to study the single-stranded DNA (ssDNA) translocation through graphene nanopores at a controllable speed. The ssDNA is pulled by an elastic force similar to the manipulation by an AFM tip. At the same time, an electric field is applied across the reservoir along the direction of the pulling force, in order to hold the ssDNA strand taut and drive the ions in the solutions through the nanopore. By monitoring and analyzing the average ionic current blockage of poly(dA)10, poly(dC)10, poly(dG)10 and poly(dT)10, it is found that one can indeed discriminate the different DNA bases from each other by holding each nucleotide in the pore for sufficiently long time. It is obtained the average blocked ionic currents can be listed, in a increasing order, as IG<IA<IT, which is almost in agreement with the order of sizes of the four nucleotides (VG>VA>VT>VC), apart from C. The results indicate that physical occupancy of the nucleotide plays the major role in affecting average blocked ionic current when the DNA translocation speed is effectively slowed down. This work provides a clue for the further investigation to realize the discrimination of the four nucleotides by the method of actively controlling DNA molecule translocation speed through the nanopores.
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