Academic literature on the topic 'Transmissibility'

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Journal articles on the topic "Transmissibility"

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Lage, Y. E., M. M. Neves, N. M. M. Maia, and D. Tcherniak. "Force transmissibility versus displacement transmissibility." Journal of Sound and Vibration 333, no. 22 (October 2014): 5708–22. http://dx.doi.org/10.1016/j.jsv.2014.05.038.

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Farrell, Elinor, Imran Mohamed, and Iain J. Robbé. "Transmissibility of Influenza." Epidemiology 23, no. 4 (July 2012): 650. http://dx.doi.org/10.1097/ede.0b013e3182583ff8.

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Klick, Brendan, Hiroshi Nishiura, and Benjamin J. Cowling. "Transmissibility of Influenza." Epidemiology 23, no. 4 (July 2012): 650–51. http://dx.doi.org/10.1097/ede.0b013e3182590554.

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Meusch, John, and Salam Rahmatalla. "3D Transmissibility and Relative Transmissibility of Immobilized Supine Humans during Transportation." Journal of Low Frequency Noise, Vibration and Active Control 33, no. 2 (June 2014): 125–37. http://dx.doi.org/10.1260/0263-0923.33.2.125.

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Yang, Rui, Dong-Mei Wang, Ning Liang, and Yan-Feng Guo. "Maximum Vibration Transmissibility of Paper Honeycomb Sandwich Structures." International Journal of Structural Stability and Dynamics 19, no. 06 (June 2019): 1971003. http://dx.doi.org/10.1142/s0219455419710032.

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The maximum vibration transmissibility of paper honeycomb sandwich structures with different sizes of honeycomb core under various static stresses was investigated using the sine frequency sweep test. The effects of the cell length of the honeycomb, the thickness of the sandwich structure, and the static stress on the maximum vibration transmissibility were evaluated and a linear polynomial equation for evaluating the maximum vibration transmissibility was obtained. The results show that the maximum vibration transmissibility increases steadily with the increase in the cell length of the honeycomb, the thickness of the sandwich structure, and the static stress. The proposed equation for the maximum vibration transmissibility is suitable for predicting the maximum vibration transmissibility of paper honeycomb sandwich structures. In addition, the fitted three-dimensional diagrams of the effects of the factors on the maximum vibration transmissibility derived from the evaluation equation were shown to be in good agreement with the experimental results.
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Bonomo, Anthony L., and Matthew Craun. "Direct transmissibility and structural acoustics." Journal of the Acoustical Society of America 150, no. 4 (October 2021): A344. http://dx.doi.org/10.1121/10.0008527.

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Direct transmissibility is a useful concept developed for transfer path analysis of linear systems. Defined loosely as the quotient between the responses at degrees-of-freedom i and j when i is excited and all other degrees of freedom are blocked, the direct transmissibility provides information on the connectivity of a system that more conventional transmissibility quantities cannot. Despite its apparent usefulness, the application of the direct transmissibility concept to structural acoustics has been somewhat limited. In this talk, the definition of direct transmissibility will be reviewed and the utility of this concept will be demonstrated through application to a few canonical structural acoustics systems. The extension of the direct transmissibility concept from spatial to modal coordinates will also be explored. [Work supported by ONR.]
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Dondorp, Harry. "Transmissibility of Delictual Claims." Bulletin of Medieval Canon Law 33, no. 1 (2016): 145–84. http://dx.doi.org/10.1353/bmc.2016.0006.

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Corsellis, J. A. N. "THE TRANSMISSIBILITY OF DEMENTIA." British Medical Bulletin 42, no. 1 (1986): 111–14. http://dx.doi.org/10.1093/oxfordjournals.bmb.a072090.

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Cogswell, James A., and Donald E. Malen. "Low force transmissibility mount." Journal of the Acoustical Society of America 80, no. 5 (November 1986): 1563. http://dx.doi.org/10.1121/1.394337.

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Schapira, Allan. "More on Malaria Transmissibility." Parasitology Today 14, no. 7 (July 1998): 293. http://dx.doi.org/10.1016/s0169-4758(98)01259-9.

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Dissertations / Theses on the topic "Transmissibility"

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Yee, Abigail K. "Using Group Transmissibility Concepts to Compare Dissimilar Vehicle Platforms." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1250525222.

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Ma, Jingjiao. "Pathogenicity and transmissibility of novel influenza viruses." Diss., Kansas State University, 2015. http://hdl.handle.net/2097/19773.

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Doctor of Philosophy
Department of Diagnostic Medicine/Pathobiology
Wenjun Ma
Influenza A virus (IAV) is an enveloped, segmented, negative-sense RNA virus that infects avian species and mammals. Its segmented feature enables antigenic shift which can generate novel IAVs that pose a threat to animal and public health due to lack of immunity to these viruses. Pigs have been considered the “mixing vessels” of influenza A viruses to generate novel reassortant viruses that may threaten animal and public health. Therefore, it is necessary to understand the pathogenicity and transmissibility of newly emerged reassortant viruses in swine. Adding to this complexity is the newly identified bat influenza A-like viruses which have roused interest in understanding the evolutionary history and pandemic potential of bat influenza. At least 10 different genotypes of novel reassortant H3N2 IAVs with gene(s) from 2009 pandemic H1N1 [A(H1N1)pdm09] have been identified in pigs in the United States. To date, only three genotypes of these viruses have been evaluated in animal models leaving the pathogenicity and transmissibility of the other seven genotype viruses unknown. We showed that reassortant viruses with genes from A(H1N1)pdm09 are pathogenic and transmissible in pigs. Further studies showed that avian-like glycine at position 228 of the HA receptor binding site is responsible for inefficient transmission of the reassortant H3N2 IAV with five A(H1N1)pdm09 genes. Studying the recently discovered IAV-like sequences from bats has been hindered by the lack of live virus isolation or culturing. Using synthetic genomics, we successfully rescued modified bat influenza viruses that had the HA and NA coding regions replaced with two classical IAVs. Additional studies were performed with truncations on NS1 protein and substitution of a putative virulence mutation in bat influenza PB2. Virus reassortment experiments demonstrated that bat influenza has limited genetic and protein compatibility with other influenza viruses; however, it readily reassorts with another divergent bat influenza virus. Taken together, our results provide insights into the pathogenicity and transmissibility of novel reassortant H3N2 IAVs in pigs. It also indicates that the bat influenza viruses recently identified are viable viruses that pose little pandemic threat to humans. Moreover, they provide new insights into the evolution and basic biology of influenza viruses.
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Lin, Juhn-Shyue. "Experimental analysis of dynamic force transmissibility through bearings." Connect to resource, 1989. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1207597697.

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Chilakapati, Sindhura, and Sri Lakshmi Jyothirmai Mamidala. "Study of Vibration Transmissibility of Operational Industrial Machines." Thesis, Blekinge Tekniska Högskola, Institutionen för tillämpad signalbehandling, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-13906.

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Industrial machines during their operation generate vibration due to dynamic forces acting on the machines. This vibration may create noise, abrasion in the machine parts, mechanical fatigue, degrade performance, transfer to other machines via floor or walls and may cause complete shutdown of the machine. To limit the vibration pre-installation, vibration isolation measures are usually employed in workshops and industrial units. However, such vibration isolation may not be sufficient due to varying operating and physical conditions, such as machine ageing, structural changes and new installations etc. Therefore, it is important to assess the quantity of vibration generated and transmitted during true operating conditions. The thesis work is aimed at the estimation of vibrational transmissibility or transfer from industrial machines to floor and to other adjacent installed machines. This study of transmissibility is based on the measurement and analysis of various spectral estimation tools such as Power Spectral Density (PSD), Frequency Response Function (FRF) and Coherence Function. The overall study is divided into three major steps. Firstly, the initial measurements are carried in BTH on simple Single Degree of Freedom (SDOF) systems to gain confidence in measurement and analysis. Then the measurements are performed on a Lathe machine “Quick Turn Nexus 300-II” in a laboratory at BTH. Finally, the measurements are taken on the machines of an Industrial workshop (KOSAB). The analysis results revealed that vibration measurements in industry are challenging and not easy as measurement in labs. Measurements are contaminated by noise from other machines, which degrade the coherence function. However, vibration transferred from one machine to the floor or other machines may be studied using FRF and PSD. Appropriate further isolations may be employed based on the spectral analysis.
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Kennedy, I. "Vibration transmissibility characteristics of fibre and steel reinforced flexible pipes." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378334.

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Imai, Natsuko. "Refining baseline estimates of dengue transmissibility and implications for control." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/43961.

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Climate change, globalisation and increased travel, increasing urban populations, overcrowding, continued poverty, and the breakdown of public health infrastructure are among the factors contributing to the 30-fold increase in total dengue incidence in the past 50 years. Consequently, with an estimated 40% of the world's population at risk of infection, dengue is now the world's most important mosquito-borne viral infection. However estimates of dengue transmissibility and burden remain ambiguous. Since the majority of infections are asymptomatic, surveillance systems substantially underestimate true rates of infection. With advances in the development of novel control measures and the recent licensing of the Sanofi Dengvaxia® dengue vaccine, obtaining robust estimates of average dengue transmission intensity is key for estimating both the burden of disease from dengue and the likely impact of interventions. Given the highly spatially heterogeneous nature of dengue transmission, future planning, implementation, and evaluation of control programs are likely to require a spatially targeted approach. Here we collate existing age-stratified seroprevalence and incidence data and develop catalytic models to estimate the burden of dengue as quantified by the force of infection and basic reproduction number. We identified a paucity of serotype-specific age stratified seroprevalence surveys in particular but showed that non-serotype specific data could give robust estimates of baseline transmission. Chapters explore whether estimates derived from different data types are comparable. Using these estimates we mapped the estimated number of dengue cases across the globe at a high spatial resolution allowing us to assess the likely impact of targeted control measures.
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Zhang, Xiaolu. "Measurement and modelling of seating dynamics to predict seat transmissibility." Thesis, University of Southampton, 2014. https://eprints.soton.ac.uk/370542/.

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The transmissibility of a seat depends on the dynamics of both the seat and the human body. Previous studies show that the apparent mass of the body, to which much attention has been paid, has a large influence on the vibration transmissibility of a seat. The influence of the seat dynamics on the seat transmissibility has received less systematic attention. The principal objective of this study was to develop a systematic methodology using finite element methods to model the dynamic interaction between a seat and the human body so as to predict the seat transmissibility. The purpose was to understand how the foam material, the seat structure, and the seat occupant influence the vibration transmitted through seats. The effect of the foam thickness at the seat cushion and the backrest on the transmissibility was investigated experimentally in the laboratory with a SAE J826 manikin and with 12 subjects during exposure to 60-s periods of fore-and-aft and vertical vibration, respectively, in the frequency range 0.5 to 20 Hz at 0.8 ms-2 r.m.s. Increasing the thickness of the foam at the seat cushion decreased the resonance frequency of both the vertical vibration transmitted to the seat cushion and the fore-and-aft vibration transmitted to the backrest, while there was little effect of the foam thickness at the backrest. It appears that the foam at the seat cushion had a predominant effect on the transmission of the vibration. Load-deflection curves were measured at various points across the lateral and fore-and-aft centrelines of a car seat with three different loading rates: 0.5, 1.0 and 2.0 mm/s. The dynamic stiffness of the seat cushion and backrest was measured with 120-s broadband random vibration (1.5 to 15 Hz) with three static preloads and with three vibration magnitudes (0.25, 0.5, and 1.0 ms-2 r.m.s.). With the same deformation, the reaction force was greater during loading than during unloading, showing evidence of hysteresis. The stiffness increased with increasing preload force and tended to decrease with increasing magnitude of vibration, indicating the seat components were nonlinear. The dynamic stiffness was also found to be greater when the seat cushion was constrained with a leather cover than without a leather cover. The transmission of vibration from the seat base to six different positions on a car seat was investigated experimentally in the laboratory with a SAE J826 manikin and with 12 subjects exposed to 120-s periods of random vibration (0.5 to 40 Hz) at three magnitudes (0.4, 0.8, and 1.2 ms-2 r.m.s.) in the fore-and-aft and vertical directions, respectively. The transmissibility from the seat base to the seat cushion surface and frame, to the backrest surface and frame, and to the headrest surface and frame exhibited a peak around 4-5 Hz in the fore-and-aft and vertical directions, respectively. The principal resonance frequency in the transmissibilities to all locations decreased with increasing magnitude of vibration, indicating nonlinearity in the seat-occupant system. There was little effect of the seat track position on the measured seat transmissibilities. The transmissibilities with subjects and with the manikin were different. Based on the experimental studies, models of the seat cushion and the backrest assemblies were built up and calibrated separately using the measured load-deflection curves and dynamic stiffnesses. They were joined to form a complete seat model and integrated with the model of a manikin for further calibration with measured seat transmissibility. The calibrated seat model was combined with a re-calibrated existing human body model to predict the transmissibility of the seat. It was found that by combining a calibrated seat model with a calibrated human body model, and defining appropriate contacts between the two models, the vertical vibration transmissibility of a seat with an occupant can be predicted. The developed seat-occupant model could be further improved to predict fore-and-aft seat transmissibility to the backrest and the dynamic pressure distributions at the interfaces between the human body and the seat.
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Schallhorn, Charles Joseph. "Coherence-based transmissibility as a damage indicator for highway bridges." Diss., University of Iowa, 2015. https://ir.uiowa.edu/etd/2007.

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Vibration-based damage detection methods are used in structural applications to identify the global dynamic response of the system. The purpose of the work presented is to exhibit a vibration-based damage detection algorithm that calculates a damage indicator, based on limited frequency bands of the transmissibility function that have high coherence, as a metric for changes in the dynamic integrity of the structure. The methodology was tested using numerical simulation, laboratory experimentation, and field testing with success in detecting, comparatively locating, and relatively quantifying different damages while also parametrically investigating variables which have been identified as issues within similar existing methods. Throughout both the numerical and laboratory analyses, the results were used to successfully detect damage as a result of crack growth or formation of new cracks. Field results using stochastic operational traffic loading have indicated the capability of the proposed methodology in evaluating the changes in the health condition of a section of the bridge and in consistently detecting cracks of various sizes (30 to 60 mm) on a sacrificial specimen integrated with the bridge abutment and a floor beam. Fluctuations in environmental and loading conditions have been known to create some uncertainties in most damage detection processes; however, this work demonstrated that by limiting the features of transmissibility to frequency ranges of high coherence, the effect of these parameters, as compared to the effect of damage, become less significant and can be neglected for some instances. The results of additional field testing using controlled impact forces on the sacrificial specimen have reinforced the findings from the operational loading in detecting damage.
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Allemang, Matthew R. "Comparison of Automotive Structures Using Transmissibility Functions and Principal Component Analysis." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1367944783.

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Deshmukh, Shounak. "Synthesizing Uncorrelated Drive Files for MIMO Transmissibility Measurements on Road Simulators." University of Cincinnati / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1471254196.

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Books on the topic "Transmissibility"

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Leung, R. C. N. Power transmissibility of an idealised gearbox. Southampton: Institute of Sound and Vibration Research, University of Southampton, 1985.

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International Symposium on Oral AIDS (1st 1989 Montreal, Québec). Oral AIDS: Manifestations, safety measures, and questions of transmissibility : proceedings of the First International Symposium on Oral AIDS held in Montreal, June 1-3, 1989. Edited by Ajdukovic Djordje, Fonds de la recherche en santé du Québec., and Institut Armand-Frappier. New York: Elsevier, 1990.

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S, Paddan G., Griffin M. J, and Great Britain. Health and Safety Executive., eds. Standard tests for the vibration transmissibility of gloves. Sudbury: HSE Books, 1999.

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Ajdukovic, Djordje. Oral Aids: MANIFESTATIONS,SAFETY MEASURES,AND QUESTIONS OF TRANSMISSIBILITY. Edited by Djordje Ajdukovic. Prentice Hall, 1990.

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Schwerter, Stephanie, Jennifer K. Dick, and Jennifer Dick. Transmissibility and Cultural Transfer: Dimensions of Translation in the Humanities. ibidem-Verlag, 2013.

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Greaves, Ian, and Paul Hunt. Biological Incidents. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199238088.003.0009.

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Chapter 9 covers information on recognition of a biological incident, natural disease outbreaks, accidental release of pathogenic organisms, bioterrorism incidents, features of an intentional biological agent release, recognition of an intentional biological agent release, bioterrorism surveillance, and biological agent biodromes, initial management of a suspected biological agent release incident, general incident management principles, universal (standard) precautions, personal protective equipment, decontamination at scene, biological agent transmissibility and public health impact, mathematical models of infection spread, pre- and post-exposure prophylaxis, the hospital response to a biological incident, primary care, cardinal signs and tips for key biological agents, the role of hospital clinicians, and the unidentified biological agent and ‘white powder’ incidents.
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Nationally adopted international standard (NAIS standard): Mechanical vibration and shock : hand-arm vibration : method for the measurement and evaluation of the vibration transmissibility of gloves at the palm of the hand. Melville, N.Y: Standards Secretariat, Acoustical Society of America, 2002.

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Book chapters on the topic "Transmissibility"

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Hendricks, Vincent F. "Transmissibility." In Trends in Logic, 267–78. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9676-3_15.

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Duran-Aniotz, C., R. Morales, I. Moreno-Gonzalez, and C. Soto. "Amyloid-β Transmissibility." In Proteopathic Seeds and Neurodegenerative Diseases, 71–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-35491-5_6.

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Varoto, P. S., and K. G. McConnell. "Q-Transmissibility Matrix vs. Single Point Transmissibility in Test Environments." In Modal Analysis and Testing, 179–208. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4503-9_8.

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Carrella, A., D. J. Ewins, and L. Harper. "Using Transmissibility measurements for Nonlinear Identification." In Conference Proceedings of the Society for Experimental Mechanics Series, 479–90. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-9299-4_39.

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Dziedziech, Kajetan, Krzysztof Mendrok, Tadeusz Uhl, and Wiesław J. Staszewski. "Wavelet-based transmissibility for structural damage detection." In Current Perspectives and New Directions in Mechanics, Modelling and Design of Structural Systems, 1770–74. London: CRC Press, 2022. http://dx.doi.org/10.1201/9781003348443-290.

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Dziedziech, Kajetan, Krzysztof Mendrok, Tadeusz Uhl, and Wiesław J. Staszewski. "Wavelet-based transmissibility for structural damage detection." In Current Perspectives and New Directions in Mechanics, Modelling and Design of Structural Systems, 617–18. London: CRC Press, 2022. http://dx.doi.org/10.1201/9781003348450-290.

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Hendricks, Vincent F. "Knowledge Transmissibility and Pluralistic Ignorance: A First Stab." In Putting Information First, 33–44. Oxford, UK: Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9781444396836.ch3.

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Dziedziech, Kajetan, Wieslaw J. Staszewski, and T. Uhl. "Time-variant system analysis using wavelet-based transmissibility." In Advances in Mechanism and Machine Science, 4167–73. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-20131-9_415.

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Singh, Arvinder, Harkirat Singh, Ishbir Singh, and Sachin Kalsi. "Transmissibility Along Human Segments When Exposed to Vibration." In Lecture Notes in Mechanical Engineering, 227–34. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-1071-7_19.

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Schwarz, Brian, Patrick McHargue, and Mark Richardson. "ODS & Modal Testing Using a Transmissibility Chain." In Special Topics in Structural Dynamics, Volume 5, 87–99. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-75390-4_7.

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Conference papers on the topic "Transmissibility"

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Ren, Tongqun, Liang He, Meiling Hui, and Zhiyong Zhang. "The quaterninon transmissibility." In 2014 7th International Congress on Image and Signal Processing (CISP). IEEE, 2014. http://dx.doi.org/10.1109/cisp.2014.7003955.

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Balbinot, Alexandre, Adalberto Schuck, and Yuri Bruxel. "Measurement of transmissibility on individuals." In 2013 IEEE International Instrumentation and Measurement Technology Conference (I2MTC). IEEE, 2013. http://dx.doi.org/10.1109/i2mtc.2013.6555459.

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Zhang, Yuan. "Drive Point Mobility, Transmissibility and Beyond." In SAE 2011 World Congress & Exhibition. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2011. http://dx.doi.org/10.4271/2011-01-0502.

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Su, J. Hannsen. "Load transmissibility control by virtual substructures." In 1996 Symposium on Smart Structures and Materials, edited by Vasundara V. Varadan and Jagdish Chandra. SPIE, 1996. http://dx.doi.org/10.1117/12.240828.

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Yushkov, A. Y. "New Method for Connection Transmissibility Calculation." In ECMOR XIII - 13th European Conference on the Mathematics of Oil Recovery. Netherlands: EAGE Publications BV, 2012. http://dx.doi.org/10.3997/2214-4609.20143275.

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Islam, M. S., and T. Manzocchi. "The Transmissibility of Partially Connected Cells." In ECMOR XIV - 14th European Conference on the Mathematics of Oil Recovery. Netherlands: EAGE Publications BV, 2014. http://dx.doi.org/10.3997/2214-4609.20141846.

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Wang, W., and A. Gupta. "Transmissibility Scale-up In Reservoir Simulation." In Annual Technical Meeting. Petroleum Society of Canada, 1999. http://dx.doi.org/10.2118/99-34.

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Chang, Y. P. "Radial-Ply vs. Bias-Ply Tires' Transmissibility." In SAE World Congress & Exhibition. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2007. http://dx.doi.org/10.4271/2007-01-1513.

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Wang, Frank Fan. "Study of Printed Circuit Board Shock Transmissibility." In ASME 2007 InterPACK Conference collocated with the ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ipack2007-33074.

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It is always a challenge to deal with dynamic loads. Often, attempts are made to compare the peak acceleration of the shock load. However, the peak acceleration is the maximum acceleration at one frequency. It may not be the most damaging load for the electronics with a different resonant frequency. This paper is to propose a mathematical method to calculate printed circuit board deflections under shock load. The theory is the printed circuit board will deflect the most at its resonance point when there are dynamic excitations. This paper also discusses a method to use load factor or equivalent acceleration load to calculate printed circuit board deflections under dynamic loads.
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Bafouni - Kotta, Anastasia, Bart Bergen, Kris Van de Rostyne, and Pantelis Nikolakopoulos. "A Physical Model for Driveshaft Vibration Transmissibility." In Noise and Vibration Conference & Exhibition. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2021. http://dx.doi.org/10.4271/2021-01-1112.

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Reports on the topic "Transmissibility"

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Hansen, N. R., V. I. Bateman, and F. A. Brown. Shock transmissibility of threaded joints. Office of Scientific and Technical Information (OSTI), December 1996. http://dx.doi.org/10.2172/639812.

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Smith, Suzanne D., Jeanne A. Smith, and Raymond J. Newman. Vibration Transmissibility Characteristics of Occupied Suspension Seats. Fort Belvoir, VA: Defense Technical Information Center, September 2006. http://dx.doi.org/10.21236/ada462026.

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Grumet, Rebecca, and Benjamin Raccah. Identification of Potyviral Domains Controlling Systemic Infection, Host Range and Aphid Transmission. United States Department of Agriculture, July 2000. http://dx.doi.org/10.32747/2000.7695842.bard.

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Potyviruses form one of the largest and most economically important groups of plant viruses. Individual potyviruses and their isolates vary in symptom expression, host range, and ability to overcome host resistance genes. Understanding factors influencing these biological characteristics is of agricultural importance for epidemiology and deployment of resistance strategies. Cucurbit crops are subject to severe losses by several potyviruses including the highly aggressive and variable zucchini yellow mosaic virus (ZYMV). In this project we sought to investigate protein domains in ZYMV that influence systemic infection and host range. Particular emphasis was on coat protein (CP), because of known functions in both cell to cell and long distance movement, and helper component-protease (HC-Pro), which has been implicated to play a role in symptom development and long distance movement. These two genes are also essential for aphid mediated transmission, and domains that influence disease development may also influence transmissibility. The objectives of the approved BARD project were to test roles of specific domains in the CP and HC-Pro by making sequence alterations or switches between different isolates and viruses, and testing for infectivity, host range, and aphid transmissibility. These objectives were largely achieved as described below. Finally, we also initiated new research to identify host factors interacting with potyviral proteins and demonstrated interaction between the ZYMV RNA dependent RNA polymerase and host poly-(A)-binding protein (Wang et al., in press). The focus of the CP studies (MSU) was to investigate the role of the highly variable amino terminus (NT) in host range determination and systemic infection. Hybrid ZYMV infectious clones were produced by substituting the CP-NT of ZYMV with either the CP-NT from watermelon mosaic virus (overlapping, but broader host range) or tobacco etch virus (TEV) (non- overlapping host range) (Grumet et al., 2000; Ullah ct al., in prep). Although both hybrid viruses initially established systemic infection, indicating that even the non-cucurbit adapted TEV CP-NT could facilitate long distance transport in cucurbits, after approximately 4-6, the plants inoculated with the TEV-CPNT hybrid exhibited a distinct recovery of reduced symptoms, virus titer, and virus specific protection against secondary infection. These results suggest that the plant recognizes the presence of the TEV CP-NT, which has not been adapted to infection of cucurbits, and initiates defense responses. The CP-NT also appears to play a role in naturally occurring resistance conferred by the zym locus in the cucumber line 'Dina-1'. Patterns of virus accumulation indicated that expression of resistance is developmentally controlled and is due to a block in virus movement. Switches between the core and NT domains of ZYMV-NAA (does not cause veinal chlorosis on 'Dina-1'), and ZYMV-Ct (causes veinal chlorosis), indicated that the resistance response likely involves interaction with the CP-NT (Ullah and Grumet, submitted). At the Volcani Center the main thrust was to identify domains in the HC-Pro that affect symptom expression or aphid transmissibility. From the data reported in the first and second year report and in the attached publications (Peng et al. 1998; Kadouri et al. 1998; Raccah et al. 2000: it was shown that: 1. The mutation from PTK to PAK resulted in milder symptoms of the virus on squash, 2. Two mutations, PAK and ATK, resulted in total loss of helper activity, 3. It was established for the first time that the PTK domain is involved in binding of the HC-Pro to the potyvirus particle, and 4. Some of these experiments required greater amount of HC-Pro, therefore a simpler and more efficient purification method was developed based on Ni2+ resin.
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