Log in

Relevant bibliographies by topics / Transport actif / Journal articles

To see the other types of publications on this topic, follow the link: Transport actif.

Journal articles on the topic 'Transport actif'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Transport actif.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Paquin, Sophie, and Anne-Sophie Dubé. "La carte conceptuelle du transport actif urbain." Cahiers de géographie du Québec 55, no. 156 (2012): 399–428. http://dx.doi.org/10.7202/1008840ar.

Full text

Abstract:

Résumé La carte conceptuelle du transport actif en milieu urbain expose l’ensemble des déterminants qui influencent les déplacements actifs. L’environnement bâti, composé de l’aménagement du territoire et des infrastructures de la voirie, constitue un des déterminants majeurs du transport actif en milieu urbain. Cependant, une série de concepts caractérisant les systèmes de transport, les politiques publiques et les individus sont à considérer pour comprendre le phénomène. La carte conceptuelle montre aussi les impacts du transport actif et du transport motorisé sur la santé ainsi que l’effet

APA, Harvard, Vancouver, ISO, and other styles

2

Bissonnette, Pierre, Pierre-Pascal Duquette, and Jean-Yves Lapointe. "Le transport d’eau secondairement actif : mythe ou réalité ?" médecine/sciences 17, no. 11 (2001): 1224–25. http://dx.doi.org/10.1051/medsci/200117111224.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Saidla, Karl, Anne Préfontaine, Carole Clavier, and Renaud Crespin. "Le transport actif à Ottawa (Canada) face à des obstacles politiques tenaces." Lien social et Politiques, no. 78 (April 5, 2017): 171–92. http://dx.doi.org/10.7202/1039344ar.

Full text

Abstract:

Le développement du transport actif (TA), c’est-à-dire des déplacements utilitaires incluant la marche, le cyclisme et l’utilisation des transports en commun, est une stratégie utilisée par les villes pour stimuler l’activité physique. Ottawa est un exemple remarquable de succès à cet égard considérant les taux de TA dans le contexte nord-américain. Cependant, la pratique du TA y est plutôt faible si on la compare à celle des résidents des villes internationales les plus performantes, suggérant que cette difficulté à accroître les taux de TA pourrait être associée au contexte socioculturel nor

APA, Harvard, Vancouver, ISO, and other styles

4

Blouin, Chantal, Éric Robitaille, Yann Le Bodo, Nathalie Dumas, Philippe De Wals, and Johanne Laguë. "Aménagement du territoire et politiques favorables à un mode de vie physiquement actif et à une saine alimentation au Québec." Lien social et Politiques, no. 78 (April 5, 2017): 19–48. http://dx.doi.org/10.7202/1039337ar.

Full text

Abstract:

Cet article analyse les politiques et mesures publiques mises en oeuvre depuis 2006 au Québec afin que l’aménagement du territoire devienne plus propice à l’adoption d’un mode de vie physiquement actif et d’une saine alimentation. L’analyse est fondée sur un inventaire et un codage caractérisant les mesures qui tentent de modifier l’environnement physique, pour influencer l’alimentation, l’utilisation du transport actif et public, de même que la pratique d’activités physiques diverses. Nous comparons ces mesures québécoises adoptées par les autorités locales, régionales et nationales aux recom

APA, Harvard, Vancouver, ISO, and other styles

5

Barbaroux, Pierre, and Cécile Godé. "Changement technologique et transfert de compétences : une réflexion à partir du cas des équipages de transport de l’armée de l’air." Management international 16 (September 20, 2012): 57–73. http://dx.doi.org/10.7202/1012393ar.

Full text

Abstract:

Cet article explore la contribution des processus de transfert des compétences en matière de gestion du changement technologique. A partir d’une étude de cas portant sur des équipages d’avions de transport tactique de l’armée de l’air, l’article démontre que le transfert des compétences offre à l’organisation des ressources utiles permettant de gérer un changement technologique majeur. L’article montre également que la culture organisationnelle ne vient pas seulement faciliter ou limiter le transfert des compétences : elle constitue un actif intangible dont le transfert s’avère critique du poi

APA, Harvard, Vancouver, ISO, and other styles

6

Lewis, Paul, and Juan Torres. "Les parents et les déplacements entre la maison et l’école primaire : quelle place pour l’enfant dans la ville?" Enfances, Familles, Générations, no. 12 (September 10, 2010): 44–64. http://dx.doi.org/10.7202/044392ar.

Full text

Abstract:

La forme urbaine et l’organisation du système scolaire ont une influence sur la mobilité des enfants et, en particulier, sur leurs déplacements scolaires. Les habitudes, les perceptions et les choix des parents pèsent tout autant. Le présent article porte sur les relations entre les considérations et les comportements parentaux, d’une part, et les modes de déplacement des enfants lors des déplacements entre la maison et l’école, d’autre part. Il prend appui sur une enquête par questionnaire menée par le Groupe de recherche ville et mobilité auprès de 1495 parents d’élèves du primaire (écoles p

APA, Harvard, Vancouver, ISO, and other styles

7

Torres, Juan, and Paul Lewis. "Proximité et transport actif. Le cas des déplacements entre l’école et la maison à Montréal et à Trois-Rivières." Environnement Urbain 4 (November 8, 2010): c1—c15. http://dx.doi.org/10.7202/044884ar.

Full text

Abstract:

Les trajets entre la maison et l’école se font de moins en moins à pied ou à vélo. La trop grande distance est évoquée, par les parents, comme une motivation pour conduire les enfants en voiture. Or, l’école primaire est souvent une destination de proximité. Le présent article examine le rôle de la proximité dans le choix modal lors des trajets scolaires à Montréal et à Trois-Rivières. Sur la base d’une enquête menée auprès de 1495 parents, on observe une forte relation entre le type d’école et la distance des trajets, ainsi qu’un seuil de domination de la marche et du vélo sur les modes motor

APA, Harvard, Vancouver, ISO, and other styles

8

Larouche, R., and F. Trudeau. "Étude des impacts du transport actif sur la pratique d’activités physiques et la santé et de ses principaux déterminants." Science & Sports 25, no. 5 (2010): 227–37. http://dx.doi.org/10.1016/j.scispo.2010.08.001.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Prince, Stephanie A., Gregory P. Butler, Deepa P. Rao, and Wendy Thompson. "Examen rapide des études spatialisées traitant des lieux d’activité physique et de sédentarité des enfants et des adultes." Promotion de la santé et prévention des maladies chroniques au Canada 39, no. 3 (2019): 71–113. http://dx.doi.org/10.24095/hpcdp.39.3.01f.

Full text

Abstract:

Introduction Les systèmes de géolocalisation par satellite (GPS) peuvent fournir un contexte supplémentaire sur les lieux où l’activité physique (AP) et les activités sédentaires (AS) sont pratiquées, surtout lorsque les données GPS sont superposées à celles de la mesure objective du mouvement. L’objectif de cet examen rapide était de résumer les données probantes tirées d’études spatialisées faisant appel à l’utilisation simultanée de GPS et de mesures objectives de l’AP et de l’AS. Méthodologie Six bases de données ont été consultées afin de recenser les études qui faisaient appel pour quant

APA, Harvard, Vancouver, ISO, and other styles

10

Perchoux, C., J. A. Nazare, T. Benmarhnia, et al. "Étude des disparités d’éducation du quartier sur la pratique du transport actif vers le lieu de travail/étude : l’effet modérateur de la distance (une étude ACTI-Cités)." Nutrition Clinique et Métabolisme 31, no. 1 (2017): 58. http://dx.doi.org/10.1016/j.nupar.2016.10.067.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Dubos, Thomas. "Un opérateur de diffusion spatialement sélectif pour le transport d'un traceur passif ou actif par un écoulement de grande échelle." Comptes Rendus de l'Académie des Sciences - Series IIB - Mechanics 329, no. 7 (2001): 509–16. http://dx.doi.org/10.1016/s1620-7742(01)01359-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Spinney, Jamie E. L. "Aerobic Activity Preferences among Older Canadians: A Time Use Perspective." Canadian Journal on Aging / La Revue canadienne du vieillissement 32, no. 4 (2013): 443–51. http://dx.doi.org/10.1017/s0714980813000433.

Full text

Abstract:

RÉSUMÉUne population physiquement active jouit de nombreux avantages pour la santé. Cette étude visait à découvrir les préférences de l’activité aérobie chez les Canadiens âgés. Quatre cycles de données indiquant l’usage national représentatif de temps ont été fusionnées avec des informations sur la dépense d’énergie pour déterminer les taux de participation et le temps consacré en dix activités aérobiques les plus fréquemment rapportées. Les activités préférés sont dominées par les tâches ménagères (15% à 30%, la participation d’environ deux heures par jour), la marche de loisirs (15% a 30%,

APA, Harvard, Vancouver, ISO, and other styles

13

Baars, R. M. T., R. De Jong, and D. Zwart. "Les coûts et revenus de l’association de l’élevage bovin et de l’agriculture dans la Province de l’Ouest de la Zambie." Revue d’élevage et de médecine vétérinaire des pays tropicaux 49, no. 3 (1996): 243–51. http://dx.doi.org/10.19182/remvt.9521.

Full text

Abstract:

Une analyse économique a été réalisée sur 50 cheptels afin de déterminer pour chacun la répartition des gains et des dépenses entre les familles, et en quelles proportions il contribuait aux revenus de la famille par rapport aux autres revenus agricoles et à ceux provenant d'autres sources. La vente de bétail, le labour avec les bœufs, l'accroissement du cheptel et la production de lait se sont avérés être les sources de revenus les plus importantes (respectivement 26, 21, 19 et 16 % des revenus totaux bruts). Les revenus de l'abattage local, du fumier et de l'usage des bœufs pour le transport

APA, Harvard, Vancouver, ISO, and other styles

14

Lavoie, M., G. Burigusa, P. Maurice, D. Hamel, and É. Turmel. "Transport actif et sécuritaire des élèves du primaire : analyse comparée des risques de blessures associés aux déplacements des enfants en automobile, à pied et à vélo entre la maison et l’école." Maladies chroniques et blessures au Canada 34, no. 4 (2014): 212–20. http://dx.doi.org/10.24095/hpcdp.34.4.02f.

Full text

Abstract:

Introduction Les programmes de promotion du transport actif au primaire visent à contrer la sédentarité chez les jeunes en provoquant un transfert modal des déplacements en automobile vers les déplacements à pied ou à vélo auprès des enfants demeurant à une distance de l'école propice à la marche ou au vélo. Cette étude a pour objectif d'évaluer le risque de blessures lié aux déplacements à pied, à vélo et en automobile entre la maison et l'école pour les élèves du primaire de la région de Montréal, ainsi que d'évaluer l'impact d'un transfert modal des déplacements en automobile vers les dépla

APA, Harvard, Vancouver, ISO, and other styles

15

Koehoorn, M., L. Tamburic, CB McLeod, PA Demers, L. Lynd, and SM Kennedy. "Surveillance populationnelle de l'asthme chez les travailleurs de Colombie-Britannique (Canada)." Maladies chroniques et blessures au Canada 33, no. 2 (2013): 101–9. http://dx.doi.org/10.24095/hpcdp.33.2.05f.

Full text

Abstract:

Introduction Des bases de données populationnelles sur la santé ont été utilisées pour la surveillance de l'asthme chez les travailleurs de la Colombie-Britannique pour la période 1999-2003. L'objectif était d'identifier des groupes à haut risque afin d'offrir des pistes pour des études complémentaires et l'élaboration de mesures d'éducation et de prévention ayant trait à l'asthme. Méthodologie Nous avons identifié des travailleurs à l'aide de codes de cotisation à un régime d'assurance maladie payée par l'employeur dans le registre médical de la Colombie-Britannique, puis couplé pour chaque t

APA, Harvard, Vancouver, ISO, and other styles

16

Le Maire, M. "Prix Nobel de chimie 1997 : Paul D. Boyer (CA, USA), John E. Walker (Cambridge, GB), Jens Skou (Aarhus, Danemark) . L'identification de la première ATPase de type P, responsable d'un transport actif d'ions, l'ATPase Na+-K+." médecine/sciences 13, no. 12 (1997): 1528. http://dx.doi.org/10.4267/10608/595.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Stępka, Małgorzata, Fabricio Ciampolini, Mauro Cresti, and Maria Charzyńska. "Localization of actin in pollen tubes of Ornithogalum virens L." Acta Societatis Botanicorum Poloniae 68, no. 2 (2014): 97–102. http://dx.doi.org/10.5586/asbp.1999.014.

Full text

Abstract:

The germinating pollen grain (in vivo on the stigma or in vitro in germination medium) forms a pollen tube which transports the vegetative nucleus and generative cell/two sperm cells participating in the process of double fertilization. The growth of the tube and the transport of organelles and the cells occur due to two major motor systems existing in the pollen tubes of higher plants: the tubuline-dynein/kinesin and the actin-myosin system. In pollen tubes of <em>Ornithogalum virens</em> the actin filaments were labelled with TRITC-phalloidin (2 µg/ml) in the PIPES buffer and the

APA, Harvard, Vancouver, ISO, and other styles

18

Shechter, Emanuel. "Transports actifs secondaires." Biochimie 68, no. 3 (1986): 357–65. http://dx.doi.org/10.1016/s0300-9084(86)80002-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Fan, Yi, Sandeepa M. Eswarappa, Masahiro Hitomi, and Paul L. Fox. "Myo1c facilitates G-actin transport to the leading edge of migrating endothelial cells." Journal of Cell Biology 198, no. 1 (2012): 47–55. http://dx.doi.org/10.1083/jcb.201111088.

Full text

Abstract:

Addition of actin monomer (G-actin) to growing actin filaments (F-actin) at the leading edge generates force for cell locomotion. The polymerization reaction and its regulation have been studied in depth. However, the mechanism responsible for transport of G-actin substrate to the cell front is largely unknown; random diffusion, facilitated transport via myosin II contraction, local synthesis as a result of messenger ribonucleic acid localization, or F-actin turnover all might contribute. By tracking a photoactivatable, nonpolymerizable actin mutant, we show vectorial transport of G-actin in l

APA, Harvard, Vancouver, ISO, and other styles

20

Wells, William A. "A disease of actin transport?" Journal of Cell Biology 163, no. 4 (2003): 689. http://dx.doi.org/10.1083/jcb1634iti3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Diesinger, Philipp M., Nilah Monnier M. Mori, Peter Lenart, and Mark Bathe. "F-Actin Mediated Chromosome Transport." Biophysical Journal 102, no. 3 (2012): 238a. http://dx.doi.org/10.1016/j.bpj.2011.11.1311.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Madsen, Agnete B., Jonas R. Knudsen, Carlos Henriquez-Olguin та ін. "β-Actin shows limited mobility and is required only for supraphysiological insulin-stimulated glucose transport in young adult soleus muscle". American Journal of Physiology-Endocrinology and Metabolism 315, № 1 (2018): E110—E125. http://dx.doi.org/10.1152/ajpendo.00392.2017.

Full text

Abstract:

Studies in skeletal muscle cell cultures suggest that the cortical actin cytoskeleton is a major requirement for insulin-stimulated glucose transport, implicating the β-actin isoform, which in many cell types is the main actin isoform. However, it is not clear that β-actin plays such a role in mature skeletal muscle. Neither dependency of glucose transport on β-actin nor actin reorganization upon glucose transport have been tested in mature muscle. To investigate the role of β-actin in fully differentiated muscle, we performed a detailed characterization of wild type and muscle-specific β-acti

APA, Harvard, Vancouver, ISO, and other styles

23

Breuer, David, Jacqueline Nowak, Alexander Ivakov, Marc Somssich, Staffan Persson, and Zoran Nikoloski. "System-wide organization of actin cytoskeleton determines organelle transport in hypocotyl plant cells." Proceedings of the National Academy of Sciences 114, no. 28 (2017): E5741—E5749. http://dx.doi.org/10.1073/pnas.1706711114.

Full text

Abstract:

The actin cytoskeleton is an essential intracellular filamentous structure that underpins cellular transport and cytoplasmic streaming in plant cells. However, the system-level properties of actin-based cellular trafficking remain tenuous, largely due to the inability to quantify key features of the actin cytoskeleton. Here, we developed an automated image-based, network-driven framework to accurately segment and quantify actin cytoskeletal structures and Golgi transport. We show that the actin cytoskeleton in both growing and elongated hypocotyl cells has structural properties facilitating ef

APA, Harvard, Vancouver, ISO, and other styles

24

Grikscheit, Katharina, Olga Dolnik, Yuki Takamatsu, Ana Raquel Pereira, and Stephan Becker. "Ebola Virus Nucleocapsid-Like Structures Utilize Arp2/3 Signaling for Intracellular Long-Distance Transport." Cells 9, no. 7 (2020): 1728. http://dx.doi.org/10.3390/cells9071728.

Full text

Abstract:

The intracellular transport of nucleocapsids of the highly pathogenic Marburg, as well as Ebola virus (MARV, EBOV), represents a critical step during the viral life cycle. Intriguingly, a population of these nucleocapsids is distributed over long distances in a directed and polar fashion. Recently, it has been demonstrated that the intracellular transport of filoviral nucleocapsids depends on actin polymerization. While it was shown that EBOV requires Arp2/3-dependent actin dynamics, the details of how the virus exploits host actin signaling during intracellular transport are largely unknown.

APA, Harvard, Vancouver, ISO, and other styles

25

Tang, Elizabeth I., Will M. Lee, and C. Yan Cheng. "Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis." Endocrinology 2016, no. 1 (2016): 47–62. http://dx.doi.org/10.1210/en.2015-1962.

Full text

Abstract:

Abstract Germ cell transport across the seminiferous epithelium during spermatogenesis requires the intricate coordination of cell junctions, signaling proteins, and both actin- and microtubule (MT)-based cytoskeletons. Although the involvement of cytoskeletons in germ cell transport has been suggested, the precise mechanism(s) remains elusive. Based on growing evidencethat actin and MT interactions underlie fundamental cellular processes, such as cell motility, it is unlikely that actin- and MT-based cytoskeletons work independently to regulate germ cell transport in the testis. Using rats tr

APA, Harvard, Vancouver, ISO, and other styles

26

Ondrej, Vladan, Emilie Lukásová, Jana Krejcí, and Stanislav Kozubek. "Intranuclear trafficking of plasmid DNA is mediated by nuclear polymeric proteins lamins and actin." Acta Biochimica Polonica 55, no. 2 (2008): 307–15. http://dx.doi.org/10.18388/abp.2008_3077.

Full text

Abstract:

Functions of nuclear polymeric proteins such as lamin A/C and actin in transport of plasmid DNA were studied. The results show that the lamina plays an important role in plasmid DNA's entry into the cell nucleus from the cytoplasm. Selective disruption of lamin A/C led to a halt in plasmid DNA transport through the nuclear envelope. Inside the nucleus, plasmid DNA was frequently localized at sites with impaired genome integrity, such as DNA double-strand breaks (DSBs), occurring spontaneously or induced by ionizing radiation. Polymeric actin obviously participates in nuclear transport of plasm

APA, Harvard, Vancouver, ISO, and other styles

27

Oberhofer, Angela, Peter Spieler, Yuliya Rosenfeld, et al. "Myosin Va’s adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro." Proceedings of the National Academy of Sciences 114, no. 24 (2017): E4714—E4723. http://dx.doi.org/10.1073/pnas.1619473114.

Full text

Abstract:

Pigment organelles, or melanosomes, are transported by kinesin, dynein, and myosin motors. As such, melanosome transport is an excellent model system to study the functional relationship between the microtubule- and actin-based transport systems. In mammalian melanocytes, it is well known that the Rab27a/melanophilin/myosin Va complex mediates actin-based transport in vivo. However, pathways that regulate the overall directionality of melanosomes on the actin/microtubule networks have not yet been delineated. Here, we investigated the role of PKA-dependent phosphorylation on the activity of th

APA, Harvard, Vancouver, ISO, and other styles

28

Dunn, Brian D., Takeshi Sakamoto, Myoung-Soon S. Hong, James R. Sellers, and Peter A. Takizawa. "Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA." Journal of Cell Biology 178, no. 7 (2007): 1193–206. http://dx.doi.org/10.1083/jcb.200707080.

Full text

Abstract:

The yeast Saccharomyces cerevisiae uses two class V myosins to transport cellular material into the bud: Myo2p moves secretory vesicles and organelles, whereas Myo4p transports mRNA. To understand how Myo2p and Myo4p are adapted to transport physically distinct cargos, we characterize Myo2p and Myo4p in yeast extracts, purify active Myo2p and Myo4p from yeast lysates, and analyze their motility. We find several striking differences between Myo2p and Myo4p. First, Myo2p forms a dimer, whereas Myo4p is a monomer. Second, Myo4p generates higher actin filament velocity at lower motor density. Thir

APA, Harvard, Vancouver, ISO, and other styles

29

Chetta, Joshua, James M. Love, Brian G. Bober, and Sameer B. Shah. "Bidirectional actin transport is influenced by microtubule and actin stability." Cellular and Molecular Life Sciences 72, no. 21 (2015): 4205–20. http://dx.doi.org/10.1007/s00018-015-1933-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Zicha, D. "Rapid Actin Transport During Cell Protrusion." Science 300, no. 5616 (2003): 142–45. http://dx.doi.org/10.1126/science.1082026.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

UEYAMA, H., H. NAKAYASU, and K. UEDA. "Nuclear actin and transport of RNA." Cell Biology International Reports 11, no. 9 (1987): 671–77. http://dx.doi.org/10.1016/0309-1651(87)90102-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Khaitlina, S. Yu. "Intracellular transport based on actin polymerization." Biochemistry (Moscow) 79, no. 9 (2014): 917–27. http://dx.doi.org/10.1134/s0006297914090089.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Schindler, M., and L. W. Jiang. "Nuclear actin and myosin as control elements in nucleocytoplasmic transport." Journal of Cell Biology 102, no. 3 (1986): 859–62. http://dx.doi.org/10.1083/jcb.102.3.859.

Full text

Abstract:

Fluorescence redistribution after photobleaching (FRAP) was used to examine the role of actin and myosin in the transport of dextrans through the nuclear pore complex. Anti-actin antibodies added to isolated rat liver nuclei significantly reduced the flux rate of fluorescently labeled 64-kD dextrans. The addition of 3 mM ATP to nuclei, which enhances the flux rate in control nuclei by approximately 250%, had no enhancement effect in the presence of either anti-actin or anti-myosin antibody. Phalloidin (10 microM) and cytochalasin D (1 micrograms/ml) individually inhibited the ATP stimulation o

APA, Harvard, Vancouver, ISO, and other styles

34

Lombardo, Andrew T., Shane R. Nelson, Guy G. Kennedy, Kathleen M. Trybus, Sam Walcott, and David M. Warshaw. "Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity." Proceedings of the National Academy of Sciences 116, no. 17 (2019): 8326–35. http://dx.doi.org/10.1073/pnas.1901176116.

Full text

Abstract:

The cell’s dense 3D actin filament network presents numerous challenges to vesicular transport by teams of myosin Va (MyoVa) molecular motors. These teams must navigate their cargo through diverse actin structures ranging from Arp2/3-branched lamellipodial networks to the dense, unbranched cortical networks. To define how actin filament network organization affects MyoVa cargo transport, we created two different 3D actin networks in vitro. One network was comprised of randomly oriented, unbranched actin filaments; the other was comprised of Arp2/3-branched actin filaments, which effectively po

APA, Harvard, Vancouver, ISO, and other styles

35

Chakrabarty, Nilaj, Pankaj Dubey, Yong Tang, et al. "Processive flow by biased polymerization mediates the slow axonal transport of actin." Journal of Cell Biology 218, no. 1 (2018): 112–24. http://dx.doi.org/10.1083/jcb.201711022.

Full text

Abstract:

Classic pulse-chase studies have shown that actin is conveyed in slow axonal transport, but the mechanistic basis for this movement is unknown. Recently, we reported that axonal actin was surprisingly dynamic, with focal assembly/disassembly events (“actin hotspots”) and elongating polymers along the axon shaft (“actin trails”). Using a combination of live imaging, superresolution microscopy, and modeling, in this study, we explore how these dynamic structures can lead to processive transport of actin. We found relatively more actin trails elongated anterogradely as well as an overall slow, an

APA, Harvard, Vancouver, ISO, and other styles

36

Herrera-Martínez, M., V. I. Hernández-Ramírez, A. E. Lagunes-Guillén, B. Chávez-Munguía, and P. Talamás-Rohana. "Actin, RhoA, and Rab11 Participation during Encystment inEntamoeba invadens." BioMed Research International 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/919345.

Full text

Abstract:

In the genusEntamoeba, actin reorganization is necessary for cyst differentiation; however, its role is still unknown. The aim of this work was to investigate the role of actin and encystation-related proteins duringEntamoeba invadensencystation. Studied proteins were actin, RhoA, a small GTPase involved through its effectors in the rearrangement of the actin cytoskeleton; Rab11, a protein involved in the transport of encystation vesicles; and enolase, as an encystment vesicles marker. Results showed a high level of polymerized actin accompanied by increased levels of RhoA-GTP during cell roun

APA, Harvard, Vancouver, ISO, and other styles

37

Durán, Juan M., Ferran Valderrama, Susana Castel, et al. "Myosin Motors and Not Actin Comets Are Mediators of the Actin-based Golgi-to-Endoplasmic Reticulum Protein Transport." Molecular Biology of the Cell 14, no. 2 (2003): 445–59. http://dx.doi.org/10.1091/mbc.e02-04-0214.

Full text

Abstract:

We have previously reported that actin filaments are involved in protein transport from the Golgi complex to the endoplasmic reticulum. Herein, we examined whether myosin motors or actin comets mediate this transport. To address this issue we have used, on one hand, a combination of specific inhibitors such as 2,3-butanedione monoxime (BDM) and 1-[5-isoquinoline sulfonyl]-2-methyl piperazine (ML7), which inhibit myosin and the phosphorylation of myosin II by the myosin light chain kinase, respectively; and a mutant of the nonmuscle myosin II regulatory light chain, which cannot be phosphorylat

APA, Harvard, Vancouver, ISO, and other styles

38

OMATA, Waka, Hiroshi SHIBATA, Lu LI, Kuniaki TAKATA, and Itaru KOJIMA. "Actin filaments play a critical role in insulin-induced exocytotic recruitment but not in endocytosis of GLUT4 in isolated rat adipocytes." Biochemical Journal 346, no. 2 (2000): 321–28. http://dx.doi.org/10.1042/bj3460321.

Full text

Abstract:

Actin-based cytoskeletons have been implicated in insulin-stimulated glucose transport and translocation of the insulin-regulated glucose transporter, GLUT4, from the intracellular pool to the plasma membrane. However, most previous studies were done using adherent cell systems such as L6 myotubes and 3T3-L1 adipocytes, and very little information is available on the significance of the actin filaments to the insulin action in isolated adipocytes, a widely used experimental system. In the present study, we investigated the physiological role of actin filaments in the subcellular trafficking of

APA, Harvard, Vancouver, ISO, and other styles

39

Niu, Fengfeng, Kang Sun, Wenjie Wei, Cong Yu, and Zhiyi Wei. "F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis." Science Advances 6, no. 45 (2020): eabb1307. http://dx.doi.org/10.1126/sciadv.abb1307.

Full text

Abstract:

Motor-mediated intracellular trafficking requires motors to position cargoes at proper locations. Myosin Va (MyoVa), an actin-based motor, is a classic model for studying cargo transport. However, the molecular basis underlying cargo unloading in MyoVa-mediated transport has remained enigmatic. We have identified MICAL1, an F-actin disassembly regulator, as a binding partner of MyoVa and shown that MICAL1-MyoVa interaction is critical for localization of MyoVa at the midbody. By binding to MICAL1, MyoVa-mediated transport is terminated, resulting in vesicle unloading at the midbody for efficie

APA, Harvard, Vancouver, ISO, and other styles

40

Bricker, Jeremy L., Shaoyou Chu, and Stephen A. Kempson. "Disruption of F-actin stimulates hypertonic activation of the BGT1 transporter in MDCK cells." American Journal of Physiology-Renal Physiology 284, no. 5 (2003): F930—F937. http://dx.doi.org/10.1152/ajprenal.00289.2002.

Full text

Abstract:

Many membrane transport systems are altered by changes in the state of the actin cytoskeleton. Although an intact microtubule network is required for hypertonic activation of the betaine transporter (BGT1), the possible role of the actin cytoskeleton is unknown. BGT1 function in Madin-Darby canine kidney cell monolayers was assessed as Na+-dependent uptake of GABA, following disassembly of F-actin by cytochalasin D (1.0 μM) or latrunculin A (0.6 μM). Both drugs significantly increased ( P < 0.001) the activation of BGT1 transport by 24-h hypertonicity (500 mosmol/kgH2O). In contrast, the hy

APA, Harvard, Vancouver, ISO, and other styles

41

Albertson, Roger, Jian Cao, Tao-shih Hsieh, and William Sullivan. "Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle." Journal of Cell Biology 181, no. 5 (2008): 777–90. http://dx.doi.org/10.1083/jcb.200803096.

Full text

Abstract:

During cytokinesis, cleavage furrow invagination requires an actomyosin-based contractile ring and addition of new membrane. Little is known about how this actin and membrane traffic to the cleavage furrow. We address this through live analysis of fluorescently tagged vesicles in postcellularized Drosophila melanogaster embryos. We find that during cytokinesis, F-actin and membrane are targeted as a unit to invaginating furrows through formation of F-actin–associated vesicles. F-actin puncta strongly colocalize with endosomal, but not Golgi-derived, vesicles. These vesicles are recruited to th

APA, Harvard, Vancouver, ISO, and other styles

42

Muriel, Olivia, Alejandra Tomas, Cameron C. Scott, and Jean Gruenberg. "Moesin and cortactin control actin-dependent multivesicular endosome biogenesis." Molecular Biology of the Cell 27, no. 21 (2016): 3305–16. http://dx.doi.org/10.1091/mbc.e15-12-0853.

Full text

Abstract:

We used in vivo and in vitro strategies to study the mechanisms of multivesicular endosome biogenesis. We found that, whereas annexinA2 and ARP2/3 mediate F-actin nucleation and branching, respectively, the ERM protein moesin supports the formation of F-actin networks on early endosomes. We also found that moesin plays no role during endocytosis and recycling to the plasma membrane but is absolutely required, much like actin, for early-to-late-endosome transport and multivesicular endosome formation. Both actin network formation in vitro and early-to-late endosome transport in vivo also depend

APA, Harvard, Vancouver, ISO, and other styles

43

Geitmann, Anja, and Andreas Nebenführ. "Navigating the plant cell: intracellular transport logistics in the green kingdom." Molecular Biology of the Cell 26, no. 19 (2015): 3373–78. http://dx.doi.org/10.1091/mbc.e14-10-1482.

Full text

Abstract:

Intracellular transport in plant cells occurs on microtubular and actin arrays. Cytoplasmic streaming, the rapid motion of plant cell organelles, is mostly driven by an actin–myosin mechanism, whereas specialized functions, such as the transport of large cargo or the assembly of a new cell wall during cell division, are performed by the microtubules. Different modes of transport are used, fast and slow, to either haul cargo over long distances or ascertain high-precision targeting, respectively. Various forms of the actin-specific motor protein myosin XI exist in plant cells and might be invol

APA, Harvard, Vancouver, ISO, and other styles

44

Qian, Xiaojing, Dolores D. Mruk, Yan-Ho Cheng, et al. "Actin binding proteins, spermatid transport and spermiation." Seminars in Cell & Developmental Biology 30 (June 2014): 75–85. http://dx.doi.org/10.1016/j.semcdb.2014.04.018.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Stamnes, Mark. "Regulating the actin cytoskeleton during vesicular transport." Current Opinion in Cell Biology 14, no. 4 (2002): 428–33. http://dx.doi.org/10.1016/s0955-0674(02)00349-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Previs, Samantha Beck, Carol S. Bookwalter, Kathleen M. Trybus, and David M. Warshaw. "Myosin Va Cargo Transport on Actin Bundles." Biophysical Journal 98, no. 3 (2010): 563a. http://dx.doi.org/10.1016/j.bpj.2009.12.3051.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Saltini, Marco, and Bela M. Mulder. "Microtubule-based actin transport and localization in a spherical cell." Royal Society Open Science 7, no. 11 (2020): 201730. http://dx.doi.org/10.1098/rsos.201730.

Full text

Abstract:

The interaction between actin filaments and microtubules is crucial for many eukaryotic cellular processes, such as, among others, cell polarization, cell motility and cellular wound healing. The importance of this interaction has long been recognized, yet very little is understood about both the underlying mechanisms and the consequences for the spatial (re)organization of the cellular cytoskeleton. At the same time, understanding the causes and the consequences of the interaction between different biomolecular components are key questions for in vitro research involving reconstituted biomole

APA, Harvard, Vancouver, ISO, and other styles

48

Kuroda, Taruho S., Hiroyoshi Ariga, and Mitsunori Fukuda. "The Actin-Binding Domain of Slac2-a/Melanophilin Is Required for Melanosome Distribution in Melanocytes." Molecular and Cellular Biology 23, no. 15 (2003): 5245–55. http://dx.doi.org/10.1128/mcb.23.15.5245-5255.2003.

Full text

Abstract:

ABSTRACT Melanosomes containing melanin pigments are transported from the cell body of melanocytes to the tips of their dendrites by a combination of microtubule- and actin-dependent machinery. Three proteins, Rab27A, myosin Va, and Slac2-a/melanophilin (a linker protein between Rab27A and myosin Va), are known to be essential for proper actin-based melanosome transport in melanocytes. Although Slac2-a directly interacts with Rab27A and myosin Va via its N-terminal region (amino acids 1 to 146) and the middle region (amino acids 241 to 405), respectively, the functional importance of the putat

APA, Harvard, Vancouver, ISO, and other styles

49

Johnson, Jennifer L., Jlenia Monfregola, Gennaro Napolitano, William B. Kiosses, and Sergio D. Catz. "Vesicular trafficking through cortical actin during exocytosis is regulated by the Rab27a effector JFC1/Slp1 and the RhoA-GTPase–activating protein Gem-interacting protein." Molecular Biology of the Cell 23, no. 10 (2012): 1902–16. http://dx.doi.org/10.1091/mbc.e11-12-1001.

Full text

Abstract:

Cytoskeleton remodeling is important for the regulation of vesicular transport associated with exocytosis, but a direct association between granular secretory proteins and actin-remodeling molecules has not been shown, and this mechanism remains obscure. Using a proteomic approach, we identified the RhoA-GTPase–activating protein Gem-interacting protein (GMIP) as a factor that associates with the Rab27a effector JFC1 and modulates vesicular transport and exocytosis. GMIP down-regulation induced RhoA activation and actin polymerization. Importantly, GMIP-down-regulated cells showed impaired ves

APA, Harvard, Vancouver, ISO, and other styles

50

Leduc, Cécile, and Sandrine Etienne-Manneville. "Regulation of microtubule-associated motors drives intermediate filament network polarization." Journal of Cell Biology 216, no. 6 (2017): 1689–703. http://dx.doi.org/10.1083/jcb.201607045.

Full text

Abstract:

Intermediate filaments (IFs) are key players in the control of cell morphology and structure as well as in active processes such as cell polarization, migration, and mechanoresponses. However, the regulatory mechanisms controlling IF dynamics and organization in motile cells are still poorly understood. In this study, we investigate the mechanisms leading to the polarized rearrangement of the IF network along the polarity axis. Using photobleaching and photoconversion experiments in glial cells expressing vimentin, glial fibrillary acidic protein, and nestin, we show that the distribution of c

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!