Journal articles: 'Trichoderma reesei'

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Schmoll, Monika. "Trichoderma reesei." Trends in Microbiology 30, no. 4 (2022): 403–4. http://dx.doi.org/10.1016/j.tim.2021.12.008.

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Jasim Mahi, Aymen, and Yasir Naser Alhamiri. "First record of Fusarium brachygibbosum as a causal agent of seed decay and damping-off disease on cotton in Iraq and Control using some bioagents." Bionatura 8, no. 4 (2023): 1–15. http://dx.doi.org/10.21931/rb/2023.08.04.63.

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The results of the isolation and identification of pathogens accompanying the symptomatic seeds and seedlings of cotton showed that the most common fungus was Fusarium brachygibbosum. Molecular identification of the studied fungus was performed using the universal primers: the results of the genetic analysis revealed the identities of the fungus as follows: a 100% identity for F. brachygibbosum that was deposited at the GenBank under accession number ON738702.1. This fungus has shown high pathogenicity against cotton seeds and seedlings by severely reducing their Germination and growth and treating cotton seeds with the biological factors of Trichoderma spp. It revealed a high efficiency in reducing disease incidence and increasing cotton germination percentage. Trichoderma viride showed the highest ability to increase seed germination to 94.44%. In comparison, the lowest ability reached 77.77 % in Trichoderma pseudokoningii and Trichoderma reesei—the results of extracting toxins from the filters of Trichoderma spp. The study's use of trichodermin and Gliotoxin showed the presence of trichodermin and Gliotoxin in large quantities. The percentage of toxin inhibition was significant against the growth of pathogenic fungi. The highest percentage of inhibition was 86.1% for the isolate Trichoderma koningiopsis, and the lowest percentage was 66.65% for the isolate Trichoderma reesei. As for the effect of isolates of resistant fungus on the Pathogen in the field, the highest germination rate was 100%, and the inhibition rate was 0.00% when using the biological preparation prepared from the isolates (T. viride, T. pseudokoningii, T. koningiopsis and T. reesei). Keywords: Fusarium brachygibbosum; Trichoderma spp.; Trichodermin; gliotoxin; Biological control.

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Febrianti, Nurul Husnah. "(The Interactive Effect between Fermentation with Trichoderma reesei on Ammoniation on Fiber Component of Sugar Palm Pulp." Bulletin of Applied Animal Research 2, no. 2 (2020): 56–60. http://dx.doi.org/10.36423/baar.v2i2.468.

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The purpose of this research was to examine the combination effect of fermentation time by Trichoderma reesei and ammoniation on fiber component of sugar palm pulp. The research used sugar palm pulp from Boyolali which was sun-dried. Sugar palm pulp fermentation with Trichoderma reesei requires nitrogen for microbial growth. Ammoniation process could loose lignocellulosic bonds and provides the nitrogen supply for Trichoderma reesei, therefore these treatments could optimize Trichoderma reesei in the fermentation process. Samples were fermented for 0, 3 and 6 days with 1,5% Trichoderma reesei. The research treatments were allocated according to a completely randomize design with pattern of 2x3 factorial. First factor was ammoniation treatment and second factor was variation of fermentation time. The parameters observedd were NDF, ADF, hemicellulose, cellulose and lignin. Research result showed that there is was no interactive effect between treatments on reducing levels of NDF and ADF, but interaction treatments decreased (P<0.05) levels of, cellulose and lignin. In conclusion, the interactive effet between fermentation time with Trichoderma reesei and ammoniation reduce levels of hemicellulose, cellulose and lignin in sugar palm pulp.

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Jasim Mahdi, Aymen, and Yasir Naser Alhamiri. "Evaluation of the Efficacy of Trichoderma species and their Fungal Toxins in the Eradication of Alternaria alternata Causing Seeds Decay and Damping-off Disease on Cotton in Iraq." Bionatura 8, no. 4 (2023): 1–14. http://dx.doi.org/10.21931/rb/2023.08.04.64.

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This study aimed to isolate and identify the pathogens accompanying the rotting and death of cotton seeds and seedlings. Also, some Trichoderma spp. were assessed against the fungal pathogen associated with the disease. The results showed that one of the essential isolated fungi, Alternaria alternata, has demonstrated high virulence in attacking cotton seeds and seedlings and reducing germination and growth. This fungus was identified based on its morphological and molecular characteristics. The Trichoderma species applied have shown high efficiency in reducing infection rates and increasing cotton germination percentage. Every isolate of Trichoderma showed a high efficiency against the fungus A. alternata by providing the highest antagonistic ability, reaching 93.75%. The highest percentage of inhibition growth of the pathogen (86.11%) was achieved by Trichoderma koningiopsis, while the lowest percentage of inhibition growth of the pathogen was 66.65 % for Trichoderma reesei. However, the biological formula prepared from species Trichoderma viride, Trichoderma pseudokoningii, Trichoderma koningiopsis and Trichoderma reesei displayed the highest percentage of inhibition of 100% against the fungus A. alternata. Keywords. Alternaria alternata; Trichoderma spp.; Trichodermin; gliotoxin; Biological control.

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Peterbauer, Clemens K., Erich Heidenreich, Ralph T. Baker, and Christian P. Kubicek. "Effect of benomyl and benomyl resistance on cellulase formation by Trichoderma reesei and Trichoderma harzianum." Canadian Journal of Microbiology 38, no. 12 (1992): 1292–97. http://dx.doi.org/10.1139/m92-213.

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To investigate the possible relationship between resistance to benomyl and the production of cellulases by Trichoderma spp., we investigated the effect of benomyl on growth and cellulase formation in Trichoderma reesei, Trichoderma harzianum, and the benomyl-resistant mutant T. harzianum T95. While T. reesei produced the highest and T. harzianum the lowest cellulase amounts, growth of both strains was equally inhibited by 2 μg/mL benomyl. However, sublethal doses of benomyl (0.2–0.5 μg/mL) promoted growth, stimulated cellulase production, and produced a highly branched, crippled morphology. The same phenomenon was observed with T. harzianum T-95, albeit at higher (5–10 μg/mL) benomyl concentrations. Introduction of the Neurospora crassa ben gene, coding for a benomyl-resistant β-tubulin, into a T. reesei by transformation yielded a series of transformants, which exhibited increased growth, increased cellulase formation, and highly branched, crippled morphology. Key words: Trichoderma harzianum, Trichoderma reesei, benomyl, rhizosphere competence, cellulase.

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BHIKHABHAI, RAMAGAURI, GUNNAR JOHANSSON, and GÖRAN PETTERSSON. "Cellobiohydrolase from Trichoderma reesei." International Journal of Peptide and Protein Research 25, no. 4 (2009): 368–74. http://dx.doi.org/10.1111/j.1399-3011.1985.tb02187.x.

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Kumakura, Minoru, Setsuko Kanno, and Kazutoshi Nisizawa. "Cellulase production in Trichoderma reesei immobilized with polymeric fibrous carriers." Canadian Journal of Microbiology 35, no. 10 (1989): 968–71. http://dx.doi.org/10.1139/m89-159.

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Trichoderma reesei mycelia were immobilized on polymeric fibrous carriers covered with various polymers, using a radiation polymerization technique. The mycelia were firmly attached to the surface of the fibril of the carrier covered with hydrophobic polymers. Cellulase production was in some cases higher by immobilized mycelia than by free mycelia and was affected by the chain structure of the polymer.Key words: Trichoderma reesei, immobilization, radiation, cellulase, fibril.

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Shobha, Balagangadharaswamy, Thimappa Ramachandrappa Lakshmeesha, Mohammad Azam Ansari, et al. "Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae." Journal of Fungi 6, no. 3 (2020): 181. http://dx.doi.org/10.3390/jof6030181.

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The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation of Trichoderma spp. (T. harzianum (PGT4), T. reesei (PGT5) and T. reesei (PGT13)) and the production of secondary metabolites from mono and co-culture and mycosynthesis of zinc oxide nanoparticles (ZnO NPs), which were characterized by a UV visible spectrophotometer, Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscope (TEM) and Selected Area (Electron) Diffraction (SAED) patterns. The fungal secondary metabolite crude was extracted from the mono and co-culture of Trichoderma spp. And were analyzed by GC-MS, which was further subjected for antibacterial activity against Xanthomonas oryzae pv. Oryzae, the causative organism for Bacterial Leaf Blight (BLB) in rice. Our results showed that the maximum zone of inhibition was recorded from the co-culture of Trichoderma spp. rather than mono cultures, which indicates that co-cultivation of beneficial fungi can stimulate the synthesis of novel secondary metabolites better than in monocultures. ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei (PGT5), Trichoderma reesei (PGT13) and co-culture (PGT4 + PGT5 + PGT13). These ZnO NPs were checked for antibacterial activity against Xoo, which was found to be of a dose-dependent manner. In summary, the biosynthesized ZnO NPs and secondary metabolites from co-culture of Trichoderma spp. are ecofriendly and can be used as an alternative for chemical fertilizers in agriculture.

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Brown, D. E., and A. J. Thornton. "Chitinous Material in Trichoderma reesei." Biotechnology Letters 20, no. 8 (1998): 777–79. http://dx.doi.org/10.1023/b:bile.0000015921.68046.d2.

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MURKOVIC, M., W. STEINER, and H. ESTERBAUER. "Electrofusion of Trichoderma reesei protoplasts." Letters in Applied Microbiology 5, no. 6 (1987): 107–9. http://dx.doi.org/10.1111/j.1472-765x.1987.tb01625.x.

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Žáková, Hana, Jiří Pazderka, Zuzana Rácová, and Pavla Ryparová. "EFFECT OF BACTERIA BACILLUS PSEUDOFIRMUS AND FUNGUS TRICHODERMA REESEI ON SELF-HEALING ABILITY OF CONCRETE." Acta Polytechnica CTU Proceedings 21 (March 21, 2019): 42–45. http://dx.doi.org/10.14311/app.2019.21.0042.

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There are usually used inorganic substances for the strengthening of self-healing ability of concrete but some specific types of bacteria or fungi may also be considered for use. The bacteria’s ability to fill pores and micro-cracks was investigated on cylindrical concrete specimens with the diameter 55 mm and height 5 mm. Bacteria Bacillus pseudofirmus and fungus Trichoderma reesei was used for the experiment. The main objective of the experiment was to investigate, if it is possible to use Bacillus pseudofirmus and Trichoderma reesei for self-healing concrete. The results show, that it is more advantageous to use Bacillus pseudofirmus than Trichoderma reesei in alkaline environment. It is desirable to create the most ideal conditions for microorganism’s growth, as possible. Bacteria should have positive effect on self-healing ability of concrete. Any effect of fungus on self-healing ability of concrete wasn’t confirmed.

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Widyaningrum, Trianik, and Masreza Parahadi. "Bioethanol Levels of Dragon Fruit (Hylocereus polyrhizus) Peel with the Addition of Blend Crude Cellulase Enzyme from Trichoderma reesei and Aspergillus niger." Journal of Tropical Biodiversity and Biotechnology 5, no. 1 (2020): 1. http://dx.doi.org/10.22146/jtbb.52189.

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The petroleum fuel crisis shows that Indonesia's fossil energy reserves are limited. It is necessary to develop an environmentally, friendly and sustainable alternative energy, one of which is bioethanol. This study aims to determine the bioethanol levels of dragon fruit (Hylocereus polyrhizus) peel with the treatment of cellulase enzymes from Trichoderma reesei and Aspergillus niger. This research was an experimental study that uses steps such as making dragon fruit peel substrate and filtrate, cellulose degradation with enzymes from Trichoderma reesei and Aspergillus niger and inoculating with yeast (Saccharomyces cerevisiae) with a fermentation time of 96 hours and then measured reducing sugar levels with the method of DNS, distillation, and the measurement of bioethanol levels using alcohol meters. The results have shown that using enzymes from Trichoderma reesei and Aspergillus niger can increase the reduction of 49.68 % sugar levels in the treatment of T.reesei: A.niger (3: 1) and produce the highest bioethanol level, which is 2.46 % in the treatment of T.reesei: A.niger (2: 1)

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Devi, Devi, Dwi Astutik, Muhammad Nur Cahyanto, and Titiek F. Djaafar. "KANDUNGAN LIGNIN, HEMISELULOSA DAN SELULOSA PELEPAH SALAK PADA PERLAKUAN AWAL SECARA FISIK KIMIA DAN BIOLOGI." Jurnal Ilmiah Rekayasa Pertanian dan Biosistem 7, no. 2 (2019): 273–82. http://dx.doi.org/10.29303/jrpb.v7i2.148.

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Penelitian ini bertujuan untuk mengetahui kandungan lignin,hemiselulosa, dan selulosa pelepah salak pada perlakuan awal secara fisik, kimia, dan biologi; serta untuk mengetahui degradasi lignin, hemiselulosa dan selulosa. Perlakuan fisik menggunakan steam explosion, perlakuan kimia menggunkan NaOH dan perlakuan biologis menggunkan Trichoderma reesei FNCC 6012. Perlakuan Pretreatment menggunkan Steam Explosion memiliki aras yaitu besarnya suhu mulai dari 1200C, 1400C, dan 1600C. Perlakuan menggunakan NaOH terdiri dari konsentrasi 2%, 4%, dan 6%, sedangkan perlakuan menggunakan Trichoderma reesei berdasarkan waktu fermentasi selama 5 hari, 10 hari, 15 hari. Perlakuan pendahuluan tersebut berfungsi untuk mengurangi kadar lignin yang ada pada pelepah salak. Parameter yang diamati dalam penelitian ini adalah kandungan lignin, hemiselulosa dan selulosa. Hasil penelitian menunjukan bahwa perlakuan menggunakan Steam Explosion dengan suhu 140 0C dan 1600C mampu menurunkan kadar lignin sebesar 16.03% dan 15,90%. Perlakuan menggunakan Steam exploision suhu 1600C dan Trichoderma reesei 15 hari mampu meningkatkan kadar Hemiselulosa sebesar 35,84 % dan 36,21%. Perlakuan menggunakan Steam Explosion dengan suhu 1600C memberikan pengaruh yang terbaik pada selulosa sebesar 51.09%.

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Wang, Xu, Liang Rong, Mingfu Wang, Yingjie Pan, Yong Zhao, and Fang Tao. "Improving the activity of endoglucanase I (EGI) from Saccharomyces cerevisiae by DNA shuffling." RSC Adv. 7, no. 73 (2017): 46246–56. http://dx.doi.org/10.1039/c6ra26508a.

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To enhance the endo-β-1,4-glucanase activity of three mixedTrichodermasp. (Trichoderma reesei, Trichoderma longibrachiatum, andTrichoderma pseudokoningii), we optimized the efficiency of the encoding gene using DNA shuffling andSaccharomyces cerevisiaeINVSc1 as a host.

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Irinyi, László, Erzsébet Sándor, and György Kövics. "Studies of Expression of Peptaibol Synthetase of Trichoderma reesei." Acta Agraria Debreceniensis, no. 16 (December 6, 2005): 188–90. http://dx.doi.org/10.34101/actaagrar/16/3310.

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Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.

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Pakula, Tiina M., Heli Nygren, Dorothee Barth, et al. "Genome wide analysis of protein production load in Trichoderma reesei." Biotechnology for Biofuels 9, no. 1 (2016): 132. https://doi.org/10.1186/s13068-016-0547-5.

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Background: The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is a widely used industrial host organism for protein production. In industrial cultivations, it can produce over 100 g/l of extracellular protein, mostly constituting of cellulases and hemicellulases. In order to improve protein production of T. reesei the transcriptional regulation of cellulases and secretory pathway factors have been extensively studied. However, the metabolism of T. reesei under protein production conditions has not received much attention.Results: To understand the physiology and metabolism of T. reesei under protein production conditions we carried out a well-controlled bioreactor experiment with extensive analysis. We used minimal media to make the data amenable for modelling and three strain pairs to cover different protein production levels. With RNA-sequencing transcriptomics we detected the concentration of the carbon source as the most important determinant of the transcriptome. As the major transcriptional response concomitant to protein production we detected the induction of selected genes that were putatively regulated by xyr1 and were related to protein transport, amino acid metabolism and transcriptional regulation. We found novel metabolic responses such as production of glycerol and a cellotriose-like compound. We then used this cultivation data for flux balance analysis of T. reesei metabolism and demonstrate for the first time the use of genome wide stoichiometric metabolic modelling for T. reesei. We show that our model can predict protein production rate and provides novel insight into the metabolism of protein production. We also provide this unprecedented cultivation and transcriptomics data set for future modelling efforts.Conclusions: The use of stoichiometric modelling can open a novel path for the improvement of protein production in T. reesei. Based on this we propose sulphur assimilation as a major limiting factor of protein production. As an organism with exceptional protein production capabilities modelling of T. reesei can provide novel insight also to other less productive organisms.

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Lee, Ching-Fu, Daniel Yuen Teh Liu, Ming Tsong Lai, and Tzong-Hsiung Hseu. "Reidentification of cellulolytic enzyme-producing Trichoderma strains W-10 and G-39." Canadian Journal of Microbiology 52, no. 6 (2006): 570–74. http://dx.doi.org/10.1139/w06-006.

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Strain W-10, originally identified as Trichoderma koningii, and its supposed mutant G-39, published for production and gene expression of cellulase and xylanase, demonstrated morphological characteristics distinct from those of T. koningii, respectively. To clarify the identification derived from morphological characteristics, several methods were used, including electrophoretic karyotyping, internal transcribed spacer (ITS) analysis of rDNA, and polymerase chain reaction (PCR) fingerprinting using the universal primer L45. All the molecular characteristics showed that strains G-39 and W-10 were identical to T. reesei and T. longibrachiatum, respectively. The results strongly supported that T. koningii G-39 and W-10 should be reassigned as T. reesei and T. longibrachiatum, respectively. Strain G-39 should be considered a mutant from T. reesei QM9414 whose spores were contaminated with those of strain W-10 during a laboratory operation. According to this, we declare that T. koningii G-39 and W-10 must be renamed as T. reesei and T. longibrachiatum, respectively.Key words: PCR fingerprinting, electrophoretic karyotypes, ITS, Trichoderma.

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Kumajas, N. J., J. S. I. T. Onibala, and N. Tuwaidan. "Pengaruh dosis inokulum dan lama inkubasi fermentasi kombinasi Phanerohaeta chrysosporium dan Trichoderma reesei terhadap kandungan nutrien eceng gondok." ZOOTEC 42, no. 2 (2022): 97. http://dx.doi.org/10.35792/zot.42.1.2022.41168.

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Eceng gondok (Eichhornia crassipes) merupakan gulma air yang tumbuh subur, sangat mudah perkembangbiakannya dan dapat tumbuh dalam kondisi apapun. Berdasarkan potensi ketersediaan dan nilai nutriennya, eceng gondok dapat dijadikan sebagai bahan pakan. Tingginya kandungan serat kasar merupakan kendala dalam penggunaannya sebagai bahan pakan unggas. Penelitian ini dilakukan untuk memperbaiki nilai nutrien eceng gondok, menggunakan metode fermentasi substrat padat dengan berbagai level dosis dan waktu inkubasi campuran inokulum Phanerochaeta chrysosporium dan Trichoderma reesei. Penelitian dilakukan dengan menggunakan rancangan acak lengkap pola faktorial. Perlakuan.terdiri dari 2 faktor yaitu dosis inokulum (D1= 2%; D2=4%; D3=6%) dan lama inkubasi (W0= 0; W1=4; W3=8 dan W4= 12 hari. Hasil penelitian menunjukan bahwa baik dosis inokulum, maupun waktu inkubasi berpengaruh sangat nyata (P<0,01) menurunkan kandungan serat kasar dan meningkatkan kandungan protein kasar. Kesimpulan dari penelitian ini adalah dosis inokulum 4% (Hanerochaeta chrysosporium 2% dan Trichoderma reesei 2%) dan waktu inkubasi 8 hari adalah dosis dan waktu inkubasi optimal menurunkan serat kasar, lignin dan meningkatkan protein kasar eceng gondok. Kata kunci: Fermentasi, Phanerohaeta chrysosporium, Trichoderma reesei, nilai gizi

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Jurys, Arnoldas, and Dalia Feizienė. "The Effect of Specific Soil Microorganisms on Soil Quality Parameters and Organic Matter Content for Cereal Production." Plants 10, no. 10 (2021): 2000. http://dx.doi.org/10.3390/plants10102000.

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Soil chemical, biological and physical properties play important roles in soil quality and are related with increasing organic matter content, soil microbiological activity, plant nutrient content and availability. A new generation of soil amendments, containing specific soil microorganisms, are of great interest worldwide. Field experiments were carried out in 2018–2019 at the Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry. The aim was to determine the impact of bio-products containing Trichoderma reesei, Acinetobacter calcoaceticus or Bacillus megaterium, and their mixtures on changes of soil organic carbon (SOC), soil respiration, and microbial biodiversity in loamy Cambisol. Under dry meteorological conditions, Trichoderma reesei, Acinetobacter calcoaceticus and Bacillus megaterium bio-products resulted an increase in SOC content, C/N ratio, humic to fulvic acid ratio, soil respiration, and microbial biodiversity. It is concluded that the use of a mixture of three microorganisms (Trichoderma reesei + Acinetobacter calcoaceticus + Bacillus megaterium) is the most promising bio-amendment under climate change. Future research is needed on different soil types and textures.

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Xin, Donglin, Ming Yang, Xiang Chen, and Junhua Zhang. "The access of Trichoderma reesei 6A to cellulose is blocked by isolated hemicelluloses and their derivatives in biomass hydrolysis." RSC Advances 6, no. 77 (2016): 73859–68. http://dx.doi.org/10.1039/c6ra14617a.

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Teeri, T. "Domain function in Trichoderma reesei cellobiohydrolases." Journal of Biotechnology 24, no. 2 (1992): 169–76. http://dx.doi.org/10.1016/0168-1656(92)90120-x.

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Nevalainen, Helena, Pirkko Suominen, and Kaarina Taimisto. "On the safety of Trichoderma reesei." Journal of Biotechnology 37, no. 3 (1994): 193–200. http://dx.doi.org/10.1016/0168-1656(94)90126-0.

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Tenkanen, Maija, Jürgen Puls, and Kaisa Poutanen. "Two major xylanases of Trichoderma reesei." Enzyme and Microbial Technology 14, no. 7 (1992): 566–74. http://dx.doi.org/10.1016/0141-0229(92)90128-b.

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Basso, Thalita Peixoto, Cláudio Rosa Gallo, and Luiz Carlos Basso. "Atividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposição." Pesquisa Agropecuária Brasileira 45, no. 11 (2010): 1282–89. http://dx.doi.org/10.1590/s0100-204x2010001100008.

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O objetivo deste trabalho foi identificar isolados de fungos a partir de bagaço de cana-de-açúcar e madeira em decomposição e avaliar a sua atividade celulolítica em bagaço de cana. Cinco isolados foram avaliados, tendo-se como referências os fungos Trichoderma reesei QM9414 e T. reesei RUT C30. A atividade celulolítica foi estimada pela capacidade hidrolítica do extrato enzimático dos fungos cultivados em bagaço de cana sobre os substratos papel de filtro (atividade celulolítica total) e carboximetilcelulose sódica (atividade da endoglucanase). Os isolados foram identificados pela análise molecular da região 26S rDNA. Os gêneros Paecilomyces, Aspergillus, Acremonium/Penicillium e Trichoderma foram identificados. Embora T. reesei QM9414 tenha apresentado a mais alta atividade celulolítica total, alguns isolados também apresentaram alta atividade de endoglucanase. A biodiversidade, em nichos como bagaço de cana-de-açúcar, pode fornecer linhagens de fungos celulolíticos com grande potencial biotecnológico.

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Zhao, Chun Hai, Xiaoyan Liu, Tong Zhan, and Jianlong He. "Production of cellulase by Trichoderma reesei from pretreated straw and furfural residues." RSC Advances 8, no. 63 (2018): 36233–38. http://dx.doi.org/10.1039/c8ra05936e.

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Santi, Sindhea Nurmala, and Trianik Widyaningrum. "PRODUKSI BIOETANOL DARI LIMBAH BATANG KELAPA SAWIT (Elaeis guineensis) MENGGUNAKAN Zymomonas mobilis DENGAN PERLAKUAN CRUDE ENZIM Trichoderma reesei DAN Aspergillus niger." Jurnal Biolokus 5, no. 1 (2022): 18. http://dx.doi.org/10.30821/biolokus.v5i1.1260.

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Bioethanol is an alternative energy that is starting to be needed, along with the depletion of petroleum reserves. Bioethanol can be obtained from various wastes, including palm oil trunk waste which contains 86.03% cellulose. The purpose of this study was to determine the production of bioethanol from oil palm trunk waste by treatment with crude enzymes Trichoderma reesei and Aspergillus niger. This research is an experimental study using a completely randomized design, namely the ratio of crude enzymes from Trichoderma reesei and Aspergillus niger (0:0), (1:0), (0:1), (1:1), (2:1), (1:2), (3:1), (1:3) on sugar and bioethanol content of fermented oil palm stem waste using Zymomonas mobilis. Sugar content was measured using the DNS method, bioethanol content was measured using an alcoholmeter. Data were analyzed using one way ANOVA. The results showed that the crude enzymes T. reesei and A. niger could increase the sugar content, namely the ratio of crude enzymes T. reesei and A. niger 3:1 (11.89 g/mL). The highest bioethanol content was in the ratio of crude enzymes T. reesei and A. niger (2:1), which was 2.34%.

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Hadiantoro, Sigit, Dyah Ratna Wulan, and Yanty Maryanty. "Study of Xylose as Product Inhibitor in Xylanase from Aspergillus niger, Basillus subtilis, and Tricodherma reesei: Insilico and Experimental Review Approach." Journal of Pure and Applied Chemistry Research 11, no. 2 (2022): 113–27. http://dx.doi.org/10.21776/ub.jpacr.2022.011.02.639.

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Bioinformatics is helpful for solving molecular biology problems computationally with the advantage of being safe, free from chemical waste, secure, cost-effective, and can shorten research time. The issues that arise can be in the form of degradation of the xylanase enzyme using some microorganisms like Aspergillus niger, Bacillus subtilis, and Trichoderma reesei on lignocellulose bonds. To predict the optimum conditions for this enzymatic reaction has used bioinformatics applications through substrate enzymes obtained from protein data banks. The purpose of this study was to determine the optimum conditions for obtaining xylanase enzymes from the microorganisms Aspergillus niger, Bacillus subtilis, and Trichoderma reesei by bioinformatics (in silico). This research was conducted in bioinformatics using a RCSB Protein Data Bank database and PubChem. The programs used to see the interaction between substrate enzymes are PyMol, PyRx, and LigPlot. The best conditions based on the results of bioinformatics simulations will form the basis for producing xylanases on a laboratory scale. In this study, the results of interaction data between Bacillus subtilis and D-xylose, Aspergillus niger with D-xylose, and Trichoderma reesei with D-xylose have a binding affinity value of -5.2 kcal/mol; -5.1 kcal/mol; and -4.3 kcal/mol respectively.

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Sinha, J., and T. Panda. "Comparative analysis of optimal endoglucanase production by Trichoderma reesei and intergeneric fusants of Trichoderma reesei Saccharomyces cerevisiae." Bioprocess Engineering 18, no. 4 (1998): 261–65. http://dx.doi.org/10.1007/pl00008988.

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Febriyani, Nila Cahyaning, and Agung Subrata. "THE EFFECT OF DURATION TIME ON ONGGOK FERMENTATION WITH ENRICHMENT N, S, P BY Trichoderma reesei ON NUTRIEN VALUE." Bulletin of Applied Animal Research 2, no. 1 (2020): 27–32. http://dx.doi.org/10.36423/baar.v2i1.226.

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The aim of this research was to study the effect of incubation time on onggok fermentation with enrichment N, S, P by Trichoderma reesei on nutrient value. The materials of this research were onggok, T. Reesei and N, S, P element. This research used completely randomized design with 4 treatments different incubation time with 5 replication; T0: 0 day, T1: 2 days, T2: 4 days and T3: 6 days. The parameters observed were crude protein, crude fat, crude fiber, and nitrogen free extract of fermented onggok. Data analysis was used by Analysis of Variance (ANOVA) and continued by Duncan’s Multiple Range test if they were significantly different. Results of this research showed that there were haven’t effect (P>0.05) of the treatment on nutrient value. The conclusion of this research was incubation time on onggok fermentation with enrichment N, S, P by Trichoderma reesei have not been able to decrease crude fiber.Keywords : Onggok, fermentation, T.reesei

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Bu, Yingjie, Bassam Alkotaini, Bipinchandra K. Salunke, Aarti R. Deshmukh, Pathikrit Saha, and Beom Soo Kim. "Direct ethanol production from cellulose by consortium of Trichoderma reesei and Candida molischiana." Green Processing and Synthesis 8, no. 1 (2019): 416–20. http://dx.doi.org/10.1515/gps-2019-0009.

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Abstract Industrial cellulosic ethanol production is a challenge due to the high cost of cellulases for hydrolysis when lignocellulosic materials are used as feedstock. In this study, direct ethanol production from cellulose was performed by consortium of Trichoderma reesei and Candida molischiana. Cellulose was hydrolyzed by a fully enzymatic saccharification process using Trichoderma reesei cellulases. The produced reducing sugar was further utilized by Candida molischiana for ethanol production. Because the optimal temperature for the cellulase system is approximately 50°C, the effect of temperature rise from 30°C to 50°C on cellulose hydrolysis was investigated. The results showed that the temperature rise from 30°C to 50°C after 36 h of cultivation was the best for reducing sugar and glucose production. Under these conditions, the maximum concentrations of reducing sugar and glucose produced by T. reesei were 8.0 g/L and 4.6 g/L at 60 h, respectively. The maximum production of ethanol by C. molischiana was 3.0 g/L after 120 h.

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Mokhtari, Wafaa. "Rhizoctoniasolani-green beans pathosystem uncover bio control efficacy of Trichoderma spp." Advances in Plants & Agriculture Research 9, no. 1 (2019): 96–99. http://dx.doi.org/10.15406/apar.2019.09.00417.

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Rhizoctoniasolani on green beans has been chosen as one of the best pathosytems to evaluate root rot diseases as well as to determine the effectiveness of potential of the biocontrol agent Trichoderma species (ssp.). In this study we investigate the effective use ofthe pathosystem model; Rhizoctoniasolani-green beans to reveal bio control efficacy of three Trichoderma spp.; T. afro-harzianum, T. reesei and T. guizouhense, isolated from Moroccan soils. In greenhouse conditions, root-dipping approach was involved in revealing bio control potential of localTrichoderma spp. by suppressing root diseases in Rhizoctonia-green beans pathosystem. Interestingly, T. reesei (T9i12) a breaking cellulose succeeded in suppressing disease incidence in root units (DI-RU) = 0.0% in green bean cultivars infected with Rhizoctoniasolani.

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Liao, Wang, Shuai Liu, Ruihong Dong, et al. "Mixed solid-state fermentation for releasing bound polyphenols from insoluble dietary fiber in carrots via Trichoderma viride and Aspergillus niger." Food & Function 13, no. 4 (2022): 2044–56. http://dx.doi.org/10.1039/d1fo03107d.

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Zeyad Khalaf Hussein, Ghada Basil Alomashi, and Khaled Yassin Zakair. "Investigation of anthraquinone-producing Trichoderma reesei by highperformance liquid chromatography (HPLC)." Journal of the Pakistan Medical Association 73, no. 9 (2023): S119—S123. http://dx.doi.org/10.47391/jpma.iq-25.

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Objective: To investigate the isolates of Trichoderma that produce anthraquinones by High-performance LiquidChromatography (HPLC).Methods: Trichoderma specimen were collected from Wassit Soil and identified dependent on morphological featuresand tested for production of anthraquinones by HPLC, Trichoderma isolate which produced a high concentration ofanthraquinones diagnosed for the species using PCR-ITS region.Results: Ten isolates were identified as Trichoderma according to morphological and microscopic features. Resultsthree Trichoderma isolates show differences between the concentrations of anthraquinones among the ten isolates.The total concentration of this compound in the extracts of specimens 1, 2 and 3 were (7.765ug/ml), (2.308ug/ml),and (4.977ug/ml) respectively. At the final concentration of Trichoderma isolates, genomic DNA have been extracted(400 to 600 ug) / (2 to 3g) fresh mycelium, and with a concentration of (1.6 to1.8), and the results of amplifyingTrichoderma DNA samples by using ITS-1 and ITS-4 showed a single unique band consistent with T. reesei F48-03, Whichwith other isolates of the Trichoderma were missing, were identified successfully.Conclusion: For identification and phylogenetic classification of Trichoderma, DNA-based methods that provide usefulclassification information are presently used. For several years, most T. spp. is regarded as a single species due to theirmorphological similarity. This research used ITS markers to distinguish genotypes within T. spp. because of amplifyinga distinct, naturally determined locus with a couple of T.reesei -specific oligonucleotide primers. This research wascarried out to provide supporting evidence for the long-standing antimicrobial use of anthraquinone.Keywords: DNA, Primers, Trichoderma, Phylogeny, Soil, Chromatography, Liquid, Polymerase, Reaction,Anthraquinones, Mycelium, Plant, Genomics.

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Blely, P., C. R. MacKenzie, and H. Schneider. "Production of acetyl xylan esterase by Trichoderma reesei and Schizophyllum commune." Canadian Journal of Microbiology 34, no. 6 (1988): 767–72. http://dx.doi.org/10.1139/m88-130.

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Activity of acetyl xylan esterase, an enzyme that removes acetyl groups from acetyl xylan, was coproduced with that of endoxylanase and endoglucanase in two Trichoderma reesei strains and one Schizophyllum commune strain. The levels of activity of extracellular enzymes were measured during the course of cultivation on different carbon sources. The highest activity levels of acetyl xylan esterase were produced by T. reesei QM 9414 in a xylan plus cellulose medium and by S. commune in a cellulose medium. Both strains produced low levels of acetyl xylan esterase activity in glucose, xylose, and cellobiose media. Schizophyllum commune also produced low levels of acetyl xylan esterase activity in xylan and acetyl xylan media. Trichoderma reesei RUT C-30 behaved like a catabolite repression resistant mutant and produced higher enzyme levels than the QM 9414 strain on all carbon sources examined. Analytical gel electrophoresis and isoelectric focusing demonstrated that the acetyl xylan esterase activity of S. commune was represented as one major form (pI 3.4) which also hydrolyzed 4-methylumbelliferyl acetate. The esterase systems of T. reesei strains were found to be more complex than those of S. commune. The pattern of coproduction of the various activities suggested that acetyl xylan esterase is a component of the cellulolytic system of the fungi tested.

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Wang, Haiyan, Ai-Ping Pang, Bingzhi Li, Liujie Huo, Fu-Gen Wu, and Fengming Lin. "Intracellular Sugar Transporters Facilitate Cellulase Synthesis in Trichoderma reesei Using Lactose." Biomolecules 13, no. 2 (2023): 295. http://dx.doi.org/10.3390/biom13020295.

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Sugar transporters play an important role in the cellulase production of lignocellulose-degrading fungi. Nevertheless, the role and function of these transporters are still unclear. Here we first report intracellular sugar transporters assisting cellulase production in Trichoderma reesei (T. reesei) using lactose. The mRNA levels of sugar transporter genes mfs, gst, and lac1 were substantially upregulated in T. reesei cultivated on lactose, with the most abundant mRNA levels at 24 h as compared to glucose. Moreover, the individual deletion of these sugar transporters significantly inhibited cellulase production, solid cell growth, and sporulation of T. reesei, suggesting they play a supporting role in cellulase production when grown in lactose. Surprisingly, MFS, GST, and LAC1 were mainly localized in the cytoplasm, with MFS and LAC1 in the endoplasmic reticulum (ER), representing the first discovery of intracellular sugar transporters involved in cellulase biosynthesis in lactose culture. The absence of the gene lac1 noticeably inhibited most of the crucial genes related to cellulase production, including cellulase-encoding genes, transcription factors, and sugar transporters, at 24 h, which was fully relieved at 48 h or 72 h, indicating that lac1 affects cellulase production more at the early step. This research advances the understanding of the function of intracellular sugar transporters in fungi, particularly for fungal cellulase production.

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Shida, Yosuke, Akinari Morikawa, Ryoichiro Tamochi, et al. "Ultrastructure of the cellulolytic fungus Trichoderma reesei." PLANT MORPHOLOGY 27, no. 1 (2015): 15–20. http://dx.doi.org/10.5685/plmorphol.27.15.

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Tisch, Doris, and Monika Schmoll. "Targets of light signalling in Trichoderma reesei." BMC Genomics 14, no. 1 (2013): 657. http://dx.doi.org/10.1186/1471-2164-14-657.

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Koike, Hideaki, Andrea Aerts, Kurt LaButti, Igor V. Grigoriev, and Scott E. Baker. "Comparative Genomics Analysis of Trichoderma reesei Strains." Industrial Biotechnology 9, no. 6 (2013): 352–67. http://dx.doi.org/10.1089/ind.2013.0015.

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39

Heikinheimo, L., J. Buchert, A. Miettinen-Oinonen, and P. Suominen. "Treating Denim Fabrics with Trichoderma Reesei Cellulases." Textile Research Journal 70, no. 11 (2000): 969–73. http://dx.doi.org/10.1177/004051750007001106.

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Harrison, M. J., I. M. Wathugala, M. Tenkanen, N. H. Packer, and K. M. H. Nevalainen. "Glycosylation of acetylxylan esterase from Trichoderma reesei." Glycobiology 12, no. 4 (2002): 291–98. http://dx.doi.org/10.1093/glycob/12.4.291.

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Satyamurthy, Prasad, Prateek Jain, Vilas S. Karande, and Vigneshwaran Nadanathangam. "Nanocellulose induces cellulase production in Trichoderma reesei." Process Biochemistry 51, no. 10 (2016): 1452–57. http://dx.doi.org/10.1016/j.procbio.2016.06.016.

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Gusakov, Alexander V. "Alternatives to Trichoderma reesei in biofuel production." Trends in Biotechnology 29, no. 9 (2011): 419–25. http://dx.doi.org/10.1016/j.tibtech.2011.04.004.

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Poutanen, Kaisa. "An α-L-arabinofuranosidase of Trichoderma reesei". Journal of Biotechnology 7, № 4 (1988): 271–81. http://dx.doi.org/10.1016/0168-1656(88)90039-9.

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Uusitalo, Jaana M., K. M. Helena Nevalainen, Anu M. Harkki, Jonathan K. C. Knowles, and Merja E. Penttilä. "Enzyme production by recombinant Trichoderma reesei strains." Journal of Biotechnology 17, no. 1 (1991): 35–49. http://dx.doi.org/10.1016/0168-1656(91)90025-q.

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Jones, Paetrice O., and Palligarnai T. Vasudevan. "Cellulose hydrolysis by immobilized Trichoderma reesei cellulase." Biotechnology Letters 32, no. 1 (2009): 103–6. http://dx.doi.org/10.1007/s10529-009-0119-x.

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Stangl, Herbert, Franz Gruber, and Christian P. Kubicek. "Characterization of the Trichoderma reesei cbh2 promoter." Current Genetics 23, no. 2 (1993): 115–22. http://dx.doi.org/10.1007/bf00352009.

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Katenkamp, Ulrich, Hans-Egon Jacob, Gerd Kerns, and Elke Dalchow. "Hybridization of Trichoderma reesei protoplasts by electrofusion." Journal of Electroanalytical Chemistry and Interfacial Electrochemistry 276, no. 1 (1989): 57–67. http://dx.doi.org/10.1016/0022-0728(89)87253-5.

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Katenkamp, Ulrich, Hans-Egon Jacob, Gerd Kerns, and Elke Dalchow. "Hybridization of Trichoderma reesei protoplasts by electrofusion." Bioelectrochemistry and Bioenergetics 22, no. 1 (1989): 57–67. http://dx.doi.org/10.1016/0302-4598(89)85030-5.

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Morikawa, Yasushi, Mikio Kawamori, Yutaka Ado, Yoriko Shinsha, Fumiko Oda, and Seigo Takasawa. "Improvement of Cellulase Production in Trichoderma reesei." Agricultural and Biological Chemistry 49, no. 6 (1985): 1869–71. http://dx.doi.org/10.1080/00021369.1985.10866993.

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Kurzatkowski, W., A. Törrönen, J. Filipek, et al. "Glucose-induced secretion of Trichoderma reesei xylanases." Applied and environmental microbiology 62, no. 8 (1996): 2859–65. http://dx.doi.org/10.1128/aem.62.8.2859-2865.1996.

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Journal articles on the topic 'Trichoderma reesei'

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