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Journal articles on the topic 'Trichomonase'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

KUTISOVA, K., J. KULDA, I. CEPICKA, et al. "Tetratrichomonads from the oral cavity and respiratory tract of humans." Parasitology 131, no. 3 (2005): 309–19. http://dx.doi.org/10.1017/s0031182005008000.

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To clarify the taxonomy of trichomonads associated with human respiratory diseases, we examined a collection of axenic trichomonad strains isolated from the oral cavity and bronchi of patients from pulmonary diseases clinics in Tallin, Estonia. The oral and bronchial strains were compared mutually as well as with a reference strain of Trichomonas tenax, a common inhabitant of the human oral cavity, and other trichomonad species from humans and animals. Unexpectedly, the morphological studies, as well as DNA sequencing of ITS1-5.8S rRNA-ITS2 regions revealed that the Estonian strains belong to

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TASCA, T., C. D. BONAN, G. A. DE CARLI, J. J. F. SARKIS, and J. F. ALDERETE. "Heterogeneity in extracellular nucleotide hydrolysis among clinical isolates ofTrichomonas vaginalis." Parasitology 131, no. 1 (2005): 71–78. http://dx.doi.org/10.1017/s0031182005007377.

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Trichomonas vaginalisis a parasitic protozoan that causes trichomonosis, a sexually-transmitted disease, with serious sequelae to women and men. As the host–parasite relationship is complex, it is important to investigate biochemical aspects of the parasite that contribute to our understanding of trichomonal biology and pathogenesis. Nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), which hydrolyses extracellular ATP and ADP, and ecto-5′-nucleotidase, which hyrolyses AMP, have been characterized in laboratory isolates ofT. vaginalis. Here we show that the extracellular ATP[ratio ]ADP h

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Potamianos, Spyros, Peter R. Mason, John S. Read, and Silas Chikunguwo. "Lysis of erythrocytes by Trichomonas vaginalis." Bioscience Reports 12, no. 5 (1992): 387–95. http://dx.doi.org/10.1007/bf01121502.

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The in vitro hemolytic activity of 4 isolates of Trichomonas vaginalis was investigated. Repetitive hemolysis assays of any one isolate showed cyclical fluctuations in hemolytic activity, varying over 24 hr of continuous culture. Maximal hemolytic activity was detected using trichomonads in the lag phase of the growth cycle. Investigations showed that hemolysis was a contact-dependent phenomenon and microscopic investigation of samples showed a significant correlation between hemolysis and attachment of erythrocytes to the trichomonad surface. Quantitative data from cytoadherence assays using

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4

Lehker, M. W., R. Arroyo, and J. F. Alderete. "The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis." Journal of Experimental Medicine 174, no. 2 (1991): 311–18. http://dx.doi.org/10.1084/jem.174.2.311.

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Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically mediated by iron; parasites cultured in a low-iron medium in the presence of salts other than iron were unresponsive to changes in adherence levels. Expression of the higher adherence property, by parasites grown first in low-iron medium followed by supplementation with iron, was a function of time, and

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OZPINAR, Necati, Hulya OZPINAR, Nuraniye ERUYGUR, and Tuğba KAYA. "EVALUATION OF ANTI-TRICHOMONASE ACTIVITIES OF METHANOL EXTRACT OF HYPERICUM SCABRUM L." INTERNATIONAL REFEREED ACADEMIC JOURNAL OF SPORTS 34 (2020): 0. http://dx.doi.org/10.17363/sstb.2020.34.2.

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6

Lehker, M. W., T. H. Chang, D. C. Dailey, and J. F. Alderete. "Specific erythrocyte binding is an additional nutrient acquisition system for Trichomonas vaginalis." Journal of Experimental Medicine 171, no. 6 (1990): 2165–70. http://dx.doi.org/10.1084/jem.171.6.2165.

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Specific receptor-mediated binding by Trichomonas vaginalis of human erythrocytes was demonstrated. The ability of live parasites to internalize erythrocytes was also documented. In vitro growth assays during lipid-free and iron-limiting conditions that do not support the survival of T. vaginalis organisms showed that purified erythrocyte lipids and hemoglobin were each able to provide lipids and/or hemoglobin iron for trichomonal growth and multiplication. Parasites bound hemoglobin in a highly specific receptor-mediated fashion, and only the homologous unlabeled hemoglobin, but not lactoferr

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7

Morton, O. "Neotran® – A New Double-Active Pessary for the Treatment of Vaginitis." Journal of International Medical Research 21, no. 1 (1993): 36–46. http://dx.doi.org/10.1177/030006059302100104.

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The common causes of vaginitis have changed in recent years. Infection with Trichomonas vaginalis is much less frequent than it used to be. The most common causes of vaginitis are candidiasis and bacterial vaginosis due to infection with mixed bacterial flora. In general practice, treatment of vaginitis is often begun before microbiological confirmation of the diagnosis is available. A vaginal pessary has been formulated to provide an effective therapy in all three forms of vaginitis mentioned above. This formulation, Neotran®, is believed to be the first pessary to contain both metronidazole

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Miranda-Ozuna, Jesús F. T., Luis Alberto Rivera-Rivas, Rosa Elena Cárdenas-Guerra, et al. "Glucose-restriction increasesTrichomonas vaginaliscellular damage towards HeLa cells and proteolytic activity of cysteine proteinases (CPs), such as TvCP2." Parasitology 146, no. 9 (2019): 1156–66. http://dx.doi.org/10.1017/s0031182019000209.

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AbstractTrichomonas vaginalisinduces cellular damage to the host cells (cytotoxicity) through the proteolytic activity of multiple proteinases of the cysteine type (CPs). Some CPs are modulated by environmental factors such as iron, zinc, polyamines, etc. Thus, the goal of this study was to assess the effect of glucose onT. vaginaliscytotoxicity, proteolytic activity and the particular role of TvCP2 (TVAG_057000) during cellular damage. Cytotoxicity assays showed that glucose-restriction (GR) promotes the highest HeLa cell monolayers destruction (~95%) by trichomonads compared to those grown u

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9

Friedman, Mendel, Christina C. Tam, Jong H. Kim, et al. "Anti-Parasitic Activity of Cherry Tomato Peel Powders." Foods 10, no. 2 (2021): 230. http://dx.doi.org/10.3390/foods10020230.

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Trichomoniasis in humans, caused by the protozoal parasite Trichomonas vaginalis, is the most common non-viral sexually transmitted disease, while Tritrichomonas foetus causes trichomonosis, an infection of the gastrointestinal tract and diarrhea in farm animals and domesticated cats. As part of an effort to determine the inhibitory effects of plant-based extracts and pure compounds, seven commercially available cherry tomato varieties were hand-peeled, freeze-dried, and pounded into powders. The anti-trichomonad inhibitory activities of these peel powders at 0.02% concentration determined usi

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10

Mendoza-López, M. Remedios, Cecilia Becerril-Garcia, Loriz V. Fattel-Facenda, et al. "CP30, a Cysteine Proteinase Involved inTrichomonas vaginalis Cytoadherence." Infection and Immunity 68, no. 9 (2000): 4907–12. http://dx.doi.org/10.1128/iai.68.9.4907-4912.2000.

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ABSTRACT We describe here the participation of a Trichomonas vaginalis 30-kDa proteinase (CP30) with affinity to the HeLa cell surface in attachment of this parasite to host epithelial cells. The CP30 band is a cysteine proteinase because its activity was inhibited by E-64, a thiol proteinase inhibitor. In two-dimensional substrate gel electrophoresis of total extracts of the trichomonad isolate CNCD 147, three spots with proteolytic activity were detected in the 30-kDa region, in the pI range from 4.5 to 5.5. Two of the spots (pI 4.5 and 5.0) bound to the surfaces of fixed HeLa cells correspo

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11

Desoubeaux, Guillaume, and Adélaïde Chesnay. "Diagnostic de la trichomonose à Trichomonas vaginalis." Revue Francophone des Laboratoires 2021, no. 530 (2021): 71–75. http://dx.doi.org/10.1016/s1773-035x(21)00071-x.

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12

Kleydman, Yekaterina, Nigel Yarlett, and Thomas E. Gorrell. "Production of ammonia by Tritrichomonas foetus and Trichomonas vaginalis." Microbiology 150, no. 5 (2004): 1139–45. http://dx.doi.org/10.1099/mic.0.26939-0.

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Production of ammonia is difficult to find among the various studies of amino acid metabolism in protozoa. Several studies suggest that catabolism of arginine to ammonium is important for the growth of trichomonads. Trichomonads are amitochondriate zooflagellates that thrive under microaerophilic and anaerobic conditions. The authors were able to detect accumulation of ammonium ions and ammonia in cultures of Tritrichomonas foetus and Trichomonas vaginalis, including those resistant to metronidazole. Ammonium ions and ammonia were detected using the indophenol colorimetric method. Cells incuba

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13

Nielsen, Tyler J., Prajakta Pradhan, Andrew Brittingham, and Wayne A. Wilson. "Glycogen Accumulation and Degradation by the Trichomonads Trichomonas vaginalis and Trichomonas tenax." Journal of Eukaryotic Microbiology 59, no. 4 (2012): 359–66. http://dx.doi.org/10.1111/j.1550-7408.2012.00624.x.

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14

van Schalkwyk, Julie, and Mark H. Yudin. "Vulvovaginite : Dépistage et prise en charge de la trichomonase, de la candidose vulvovaginale et de la vaginose bactérienne." Journal of Obstetrics and Gynaecology Canada 38, no. 12 (2016): S587—S596. http://dx.doi.org/10.1016/j.jogc.2016.09.065.

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15

van Schalkwyk, Julie, Mark H. Yudin, Mark H. Yudin, et al. "Vulvovaginite: Dépistage et prise en charge de la trichomonase, de la candidose vulvovaginale et de la vaginose bactérienne." Journal of Obstetrics and Gynaecology Canada 37, no. 3 (2015): 275–76. http://dx.doi.org/10.1016/s1701-2163(15)30317-0.

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16

Alrefaei, Abdulwahed Fahad, Ross Low, Neil Hall, et al. "Multilocus Analysis Resolves the European Finch Epidemic Strain of Trichomonas gallinae and Suggests Introgression from Divergent Trichomonads." Genome Biology and Evolution 11, no. 8 (2019): 2391–402. http://dx.doi.org/10.1093/gbe/evz164.

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Abstract In Europe, Trichomonas gallinae recently emerged as a cause of epidemic disease in songbirds. A clonal strain of the parasite, first found in the United Kingdom, has become the predominant strain there and spread to continental Europe. Discriminating this epidemic strain of T. gallinae from other strains necessitated development of multilocus sequence typing (MLST). Development of the MLST was facilitated by the assembly and annotation of a 54.7 Mb draft genome of a cloned stabilate of the A1 European finch epidemic strain (isolated from Greenfinch, Chloris chloris, XT-1081/07 in 2007

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17

Calla-Choque, Jaeson Santos, Elisa Elvira Figueroa-Angulo, Leticia Ávila-González та Rossana Arroyo. "α-Actinin TvACTN3 ofTrichomonas vaginalisIs an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism". BioMed Research International 2014 (2014): 1–20. http://dx.doi.org/10.1155/2014/424767.

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Trichomonas vaginalisis a sexually transmitted flagellated protist parasite responsible for trichomoniasis. This parasite is dependent on high levels of iron, favoring its growth and multiplication. Iron also differentially regulates some trichomonad virulence properties by unknown mechanisms. However, there is evidence to support the existence of gene regulatory mechanisms at the transcriptional and posttranscriptional levels that are mediated by iron concentration inT. vaginalis. Thus, the goal of this study was to identify an RNA-binding protein inT. vaginalisthat interacts with the tvcp4 R

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18

Leon, Segundo R., Kelika A. Konda, Kyle T. Bernstein, et al. "Trichomonas vaginalisInfection and Associated Risk Factors in a Socially-Marginalized Female Population in Coastal Peru." Infectious Diseases in Obstetrics and Gynecology 2009 (2009): 1–6. http://dx.doi.org/10.1155/2009/752437.

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Objective. The epidemiology ofTrichomonas vaginalisinfection among sexually active socially-marginalized women in three urban, coastal Peruvian cities was examined in order to quantify the prevalence of trichomonas infection and identify associated risk factors.Methods. We conducted a cross-sectional, venue-based study of women from socially-marginalized populations in three coastal Peruvian cities.Results. Among the 319 women enrolled, the overall prevalence of trichomonal infection was 9.1% (95% CI, 5.9%–12.3%). The mean age was 26.3 years, and 35.5% reported having had unprotected intercour

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19

Alderete, J. F. "Iron Modulates Phenotypic Variation and Phosphorylation of P270 in Double-Stranded RNA Virus-Infected Trichomonas vaginalis." Infection and Immunity 67, no. 8 (1999): 4298–302. http://dx.doi.org/10.1128/iai.67.8.4298-4302.1999.

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ABSTRACT Trichomonas vaginalis infected with a double-stranded RNA virus undergoes phenotypic variation on the basis of surface versus cytoplasmic expression of the immunogenic protein P270. Examination of batch cultures by flow cytofluorometry with monoclonal antibody (MAb) to P270 yields both fluorescent and nonfluorescent trichomonads. Greater numbers and intensity of fluorescent organisms with surface P270 reactive with MAb were evident in parasites grown in medium depleted of iron. Placement of iron-limited organisms in medium supplemented with iron gave increased numbers of nonfluorescen

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20

Rendón-Gandarilla, Francisco Javier, Lucero de los Angeles Ramón-Luing, Jaime Ortega-López, Ivone Rosa de Andrade, Marlene Benchimol, and Rossana Arroyo. "The TvLEGU-1, a Legumain-Like Cysteine Proteinase, Plays a Key Role inTrichomonas vaginalisCytoadherence." BioMed Research International 2013 (2013): 1–18. http://dx.doi.org/10.1155/2013/561979.

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The goal of this paper was to characterize aTrichomonas vaginaliscysteine proteinase (CP) legumain-1 (TvLEGU-1) and determine its potential role as a virulence factor duringT. vaginalisinfection. A 30-kDa band, which migrates in three protein spots (pI~6.3, ~6.5, and ~6.7) with a different type and level of phosphorylation, was identified as TvLEGU-1 by one- and two-dimensional Western blot (WB) assays, using a protease-rich trichomonad extract and polyclonal antibodies produced against the recombinant TvLEGU-1 (anti-TvLEGU-1r). Its identification was confirmed by mass spectrometry. Immunofluo

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Graves, Keonte J., Jan Novak, W. Evan Secor, Patricia J. Kissinger, Jane R. Schwebke, and Christina A. Muzny. "A systematic review of the literature on mechanisms of 5-nitroimidazole resistance in Trichomonas vaginalis." Parasitology 147, no. 13 (2020): 1383–91. http://dx.doi.org/10.1017/s0031182020001237.

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AbstractBackgroundTrichomonas vaginalis is the most common non-viral sexually transmitted infection. 5-Nitroimidazoles [metronidazole (MTZ) and tinidazole (TDZ)] are FDA-approved treatments. To better understand treatment failure, we conducted a systematic review on mechanisms of 5-nitroimidazole resistance.MethodsPubMed, ScienceDirect and EMBASE databases were searched using keywords Trichomonas vaginalis, trichomoniasis, 5-nitroimidazole, metronidazole, tinidazole and drug resistance. Non-English language articles and articles on other treatments were excluded.ResultsThe search yielded 606 a

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22

Benchimol, Marlene. "Trichomonads under Microscopy." Microscopy and Microanalysis 10, no. 5 (2004): 528–50. http://dx.doi.org/10.1017/s1431927604040905.

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Trichomonads are flagellate protists, and among themTrichomonas vaginalisandTritrichomonas foetusare the most studied because they are parasites of the urogenital tract of humans and cattle, respectively. Microscopy provides new insights into the cell biology and morphology of these parasites, and thus allows better understanding of the main aspects of their physiology. Here, we review the ultrastructure ofT. foetusandT. vaginalis, stressing the participation of the axostyle in the process of cell division and showing that the pseudocyst may be a new form in the trichomonad cell cycle and not

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Martikaynen, Zinaida Mikhaylovna, Aleksey Nikolayevich Grigoryev, Olga Sergeyevna Ryzhkova, et al. "Comparison of laboratory methods for diagnosis of Trichomonas vaginalis." Journal of obstetrics and women's diseases 63, no. 1 (2014): 5–9. http://dx.doi.org/10.17816/jowd6315-9.

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The paper presents data on comparison of microscopy, culture and molecular (PCR) methods for diagnosis of trichomoniasis, as well as on evaluation of the quality of culture media for isolation of trichomonads produced by Russian and international manufacturers. The highest sensitivity was shown for the molecular (real-time PCR) method. Microscopy of stained and wet smears demonstrated relatively high sensitivity. The culture media that are widely used in our country for Trichomonas vaginalis isolation need optimization.

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SMEJKALOVÁ, PAVLA, KLÁRA J. PETRŽELKOVÁ, KATEŘINA POMAJBÍKOVÁ, DAVID MODRÝ, and IVAN ČEPIČKA. "Extensive diversity of intestinal trichomonads of non-human primates." Parasitology 139, no. 1 (2011): 92–102. http://dx.doi.org/10.1017/s0031182011001624.

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SUMMARYDespite the fact that the non-human primates are our closest relatives and represent a species-rich mammalian group, little is known about their intestinal protistan parasites/commensals. Particularly, the intestinal trichomonads represent a neglected part of the fauna of the primate digestive system. We have established 30 trichomonad strains isolated from feces of 11 primate species kept in 3 Czech zoos and performed an analysis of their SSU rDNA and ITS1-5·8S rDNA-ITS2. Our results showed that intestinal trichomonads are rather common among non-human primates. Molecular phylogenetic

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MARTY, MATHIEU, MARK BONNER, and FRÉDÉRIC VAYSSE. "Observation of trichomonads infection in a child with periodontitis by direct microscopy at the dental office." Parasitology 142, no. 11 (2015): 1440–42. http://dx.doi.org/10.1017/s0031182015000876.

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SUMMARYThe pathogenicity of Trichomonas species is well documented. Although their exact involvement in gum disease is not fully understood, recent studies suggest a correlation between these protozoa and periodontitis. This case report details the first chair-side observation in Europe of an oral trichomonad infection in a child with periodontitis, by direct microscopy. The dramatic recovery of the patient, observed following administration of an anti-parasitic treatment, confirms the necessity of further investigation in this field.

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Omer, El-Fadil E., R. D. Catterall, M. H. Ali, H. A. El-Naeem, and H. H. Erwa. "Vaginal Trichomoniasis." Tropical Doctor 15, no. 4 (1985): 170–72. http://dx.doi.org/10.1177/004947558501500407.

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At a sexually transmitted disease clinic at Khartoum 613 Sudanese women presenting with vaginal discharge were investigated. Specimens were examined by microscopy and culture. Trichomonas vaginalis infestation was found in 123 patients (20.1%), predominantly in the age groups 16–19 years (27.1%) and 46–65 years (27%). Frequency of trichomonal vaginitis was highest (35.9%) among divorced women. Of the pregnant women investigated, 16.3% were found to harbour the parasite. There was no significant difference in the parity of parasitized and non-parasitized patients.

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Brugerolle, Guy, Geneviève Bricheux, and Gérard Coffe. "Actin cytoskeleton demonstration in Trichomonas vaginalis and in other trichomonads." Biology of the Cell 88, no. 1-2 (1996): 29–36. http://dx.doi.org/10.1016/s0248-4900(97)86828-1.

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THONG, K., G. COOMBS, and B. SANDERSON. "S-adenosylhomocysteine hydrolase activity in Trichomonas vaginalis and other trichomonads." Molecular and Biochemical Parasitology 17, no. 1 (1985): 35–44. http://dx.doi.org/10.1016/0166-6851(85)90126-4.

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Dimasuay, Kris Genelyn B., Orlie John Y. Lavilla, and Windell L. Rivera. "New Hosts ofSimplicimonas similisandTrichomitus batrachorumIdentified by 18S Ribosomal RNA Gene Sequences." Journal of Parasitology Research 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/831947.

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Trichomonads are obligate anaerobes generally found in the digestive and genitourinary tract of domestic animals. In this study, four trichomonad isolates were obtained from carabao, dog, and pig hosts using rectal swab. Genomic DNA was extracted using Chelex method and the 18S rRNA gene was successfully amplified through novel sets of primers and undergone DNA sequencing. Aligned isolate sequences together with retrieved 18S rRNA gene sequences of known trichomonads were utilized to generate phylogenetic trees using maximum likelihood and neighbor-joining analyses. Two isolates from carabao w

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Mundodi, V., A. S. Kucknoor та J. F. Alderete. "Immunogenic and Plasminogen-Binding Surface-Associated α-Enolase of Trichomonas vaginalis". Infection and Immunity 76, № 2 (2007): 523–31. http://dx.doi.org/10.1128/iai.01352-07.

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ABSTRACT Trichomonas vaginalis is a protist that causes the most common human sexually transmitted infection. A T. vaginalis cDNA expression library was screened with pooled sera from patients with trichomoniasis. A highly reactive cDNA clone of 1,428 bp encoded a trichomonad protein of 472 amino acids with sequence identity to α-enolase (tv-eno1). The sequence alignment confirmed the highly conserved nature of the enzyme with 65% to 84% identity among organisms. The expression of tv-eno1 was up-regulated by contact of parasites with vaginal epithelial cells, and this is the first report demon

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Dailey, D. C., C. Te-Hung, and J. F. Alderete. "Characterization ofTrichomonas vaginalishaemolysis." Parasitology 101, no. 2 (1990): 171–75. http://dx.doi.org/10.1017/s0031182000063204.

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The haemolytic activity of liveTrichomonas vaginalisorganisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 μm filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37 °C. Treatment of trichomonads with metronidazole reduced levels of

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Journal, Baghdad Science. "Evaluation the efficiency of Trichomonas vaginalis depending on clinical sings , direct examination ,culturing and serological test." Baghdad Science Journal 8, no. 1 (2011): 392–99. http://dx.doi.org/10.21123/bsj.8.1.392-399.

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The adequacy of diagnostic tests, together with trichomoniasis associated clinical symptoms, were investigated in females suffering vaginitis, and they were referred to the Gynecology Department, Al-Yarmouk Teaching Hospital during the period December 2004 – June 2005. The total number of patients was 250 cases (age range: 18 - 52 years), and each patient was examined using a sterile speculum to obtain vaginal swabs for examination. The diagnosis with T. vaginalis was done in many methods. The direct methods included wet and stained (Leishman's stain) examinations and cultivation in different

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MIDLEJ, V., and M. BENCHIMOL. "Trichomonas vaginaliskills and eats – evidence for phagocytic activity as a cytopathic effect." Parasitology 137, no. 1 (2009): 65–76. http://dx.doi.org/10.1017/s0031182009991041.

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SUMMARYThis study reports that the cytopathic effect ofTrichomonas vaginalis, an important human parasite of the urogenital tract, occurs due to mechanical stress and subsequent phagocytosis of the necrotic cells. The investigation was done using a primary culture of bovine oviduct epithelial cells (BOECs), grown either in monolayers or as floating cells. Trophozoites displaying different virulence levels were co-incubated with BOECs for times varying between 1 min and 48 h. Analyses were performed using videomicroscopy, scanning and transmission electron microscopy, colourimetric assays and c

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Padhye, Saraswati M. "CHANGING TRENDS OF VAGINAL INFECTION." Journal of Nepal Medical Association 42, no. 145 (2003): 18–22. http://dx.doi.org/10.31729/jnma.711.

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ABSTRACTIntroductionVaginal discharge and /or infection are very distressing to all women.Vaginal infection encompasses-Vaginosis/vaginitis,Trichomonal vaginitis and Monilial or fungal infection. Vaginal discharge could be due to foreignbody such as ring pessary inserted for uterovaginal prolapse or for induction of abortion by untrained Daisin Nepal. It could be due to physiological condition, such as -during mid menstrual and pregnant state. Astudy of pattern of infection in vaginal discharge has been presented in this study.ObjectiveTo find the pattern of infection in the vaginal discharge

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Fedorich, P. V. "Protozoynye invasion of urogenital system in combine with bacterial vaginosis agents by women of fertil age and their sexual partners." HEALTH OF WOMAN, no. 7(133) (September 30, 2018): 100–106. http://dx.doi.org/10.15574/hw.2018.133.100.

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Study of protozoyny invazions of the uroganital system with combination of microorganisms which are associated with bacterial vaginosis by women of fertil age and their sexual partners men is perspective direction of modern medicine. The development of this direction can increase the level of diagnostic inspection of patients by transmissible mainly sexual way infections and improve of efficiency of their treatment. The objective: exposure of combine defeats of uroganital system with different kinds of trichomonas or lamblias and microorganisms which are associated with bacterial vaginosis, fo

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FELLEISEN, R. S. J. "Comparative sequence analysis of 5·8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa." Parasitology 115, no. 2 (1997): 111–19. http://dx.doi.org/10.1017/s0031182097001212.

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The taxonomic situation in the genus Tritrichomonas is the subject of controversial discussion: potentially T. foetus and T. suis, the tritrichomonads from cattle and swine, respectively, could belong to the same species. In order to shed some light on this question, a molecular biological analysis was performed. The 5·8S rRNA gene and the flanking internal transcribed spacer regions (ITS1 and ITS2) of 12 different isolates of 3 Tritrichomonas species T. foetus, T. suis and T. mobilensis were enzymatically amplified by PCR and subcloned. Also, the corresponding regions of the trichomonads Tric

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Zanzani, Sergio A., Alessia L. Gazzonis, Paola Scarpa, Emanuela Olivieri, Hans-Jörg Balzer, and Maria Teresa Manfredi. "Coinfection withTritrichomonas foetusandGiardia duodenalisin Two Cats with Chronic Diarrhea." Case Reports in Veterinary Medicine 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/5705168.

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A Tritrichomonas foetusandGiardia duodenalismixed infection was diagnosed in two Maine Coon cats aged six months. One of them presented a history of chronic liquid diarrhea and of several unsuccessful treatments. In both cats,G. duodenalisand trichomonads were detected in fecal smears from freshly voided feces; the presence ofT. foetuswas confirmed by a real-time PCR assay. The cats completely recovered after treatment with ronidazole. In a refrigerated fecal sample collected from the cat with chronic diarrhea, drop-shaped trichomonad pseudocysts smaller thanG. duodenaliscysts were detected. T

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Adu-Sarkodie, Y. A., H. A. Weiss, and D. C. W. Mabey. "Are trichomonads other than Trichomonas vaginalisinvolved in the aetiology of vaginal trichomoniasis?" Transactions of the Royal Society of Tropical Medicine and Hygiene 97, no. 6 (2003): 625. http://dx.doi.org/10.1016/s0035-9203(03)80061-5.

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Garcia, Ana F., M. Benchimol, and J. F. Alderete. "Trichomonas vaginalis Polyamine Metabolism Is Linked to Host Cell Adherence and Cytotoxicity." Infection and Immunity 73, no. 5 (2005): 2602–10. http://dx.doi.org/10.1128/iai.73.5.2602-2610.2005.

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ABSTRACT Trichomonas vaginalis secretes putrescine that is readily detected in vaginal secretions. We wanted to examine the effect of decreased putrescine synthesis by inhibition of ornithine decarboxylase (ODC) on T. vaginalis. One reason is because inhibition of Tritrichomonas foetus ODC results in growth arrest, destruction of hydrogenosomes, and decreased amounts of hydrogenosomal enzymes. Treatment of T. vaginalis T016 with ≥20 mM 1,4-diamino-2-butanone (DAB) to inhibit ODC resulted in growth arrest, which was reversed by addition of exogenous putrescine. No similar reversal of growth arr

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Walker, R. L., D. C. Hayes, S. J. Sawyer, R. W. Nordhausen, K. A. Van Hoosear, and R. H. BonDurant. "Comparison of the 5.8S rRNA Gene and Internal Transcribed Spacer Regions of Trichomonadid Protozoa Recovered from the Bovine Preputial Cavity." Journal of Veterinary Diagnostic Investigation 15, no. 1 (2003): 14–20. http://dx.doi.org/10.1177/104063870301500104.

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Sequence analysis of the 5.8S rRNA gene and the internal transcribed spacer regions (ITSRs) was used to compare trichomonadid protozoa ( n = 39) of varying morphologies isolated from the bovine preputial cavity. A multiple sequence alignment was performed with bovine isolate sequences and other trichomonadid protozoa sequences available in GenBank. As a group, Tritrichomonas foetus isolates ( n = 7) had nearly complete homology. A similarity matrix showed low homology between the T. foetus isolates and other trichomonads recovered from cattle (<70%). Two clusters of trichomonads other than

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Wang, A., C. C. Wang, and J. F. Alderete. "Trichomonas vaginalis phenotypic variation occurs only among trichomonads infected with the double-stranded RNA virus." Journal of Experimental Medicine 166, no. 1 (1987): 142–50. http://dx.doi.org/10.1084/jem.166.1.142.

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Trichomonas vaginalis isolates were examined for the presence of viral double-stranded RNA (dsRNA) and the property of phenotypic variation. Only the heterogeneous isolates composed of mAb-reactive and -nonreactive organisms, as determined by indirect immunofluorescence and flow cytofluorometry, and capable of phenotypic variation possessed the dsRNA. Both the positive and negative phenotype subpopulations separated from the heterogeneous parent contained equal amounts of the dsRNA. Loss of the dsRNA upon prolonged in vitro cultivation always correlated with the lack of expression of the major

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Mason, Peter R., and Lovemore Gwanzura. "Mouse Spleen Cell Responses to Trichomonal Antigens in Experimental Trichomonas vaginalis Infection." Journal of Parasitology 74, no. 1 (1988): 93. http://dx.doi.org/10.2307/3282483.

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Kucknoor, Ashwini S., Vasanthakrishna Mundodi, and J. F. Alderete. "Adherence to Human Vaginal Epithelial Cells Signals for Increased Expression of Trichomonas vaginalis Genes." Infection and Immunity 73, no. 10 (2005): 6472–78. http://dx.doi.org/10.1128/iai.73.10.6472-6478.2005.

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ABSTRACT Host parasitism by Trichomonas vaginalis is complex, and the adhesion to vaginal epithelial cells (VECs) by trichomonads is preparatory to colonization of the vagina. Since we showed increased synthesis of adhesins after contact with VECs (A. F. Garcia, et al., Mol. Microbiol. 47:1207-1224, 2003) and more recently demonstrated up-regulated gene expression in VECs after parasite attachment (A. S. Kucknoor, et al., Cell. Microbiol. 7:887-897, 2005), we hypothesized that enhanced expression of adhesin and other genes would result from signaling of trichomonads following adherence. In ord

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Kellerová, Pavlína, and Jan Tachezy. "Zoonotic Trichomonas tenax and a new trichomonad species, Trichomonas brixi n. sp., from the oral cavities of dogs and cats." International Journal for Parasitology 47, no. 5 (2017): 247–55. http://dx.doi.org/10.1016/j.ijpara.2016.12.006.

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Lehker, M. W., and J. F. Alderete. "Iron regulates growth of Trichomonas vaginalis and the expression of immunogenic trichomonad proteins." Molecular Microbiology 6, no. 1 (1992): 123–32. http://dx.doi.org/10.1111/j.1365-2958.1992.tb00844.x.

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Alderete, J. F., E. Newton, C. Dennis, and K. A. Neale. "Antibody in sera of patients infected with Trichomonas vaginalis is to trichomonad proteinases." Sexually Transmitted Infections 67, no. 4 (1991): 331–34. http://dx.doi.org/10.1136/sti.67.4.331.

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Cuvertoret-Sanz, Maria, Christiane Weissenbacher-Lang, Madeleine Lunardi, et al. "Coinfection with Entamoeba polecki and Brachyspira hyodysenteriae in a pig with severe diarrhea." Journal of Veterinary Diagnostic Investigation 31, no. 2 (2019): 298–302. http://dx.doi.org/10.1177/1040638719829800.

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Enteric disease in pigs is usually of multifactorial etiology, including infectious and non-infectious factors. In many cases of endemic diarrhea in weaner-to-finisher pigs, the combination of 2 or more microorganisms leads to aggravation of intestinal lesions and, consequently, clinical signs. We autopsied a 4-mo-old fattening pig with diarrhea and diagnosed severe fibrinonecrotizing typhlocolitis. Numerous spiral-shaped bacteria and amoeba-like PAS-positive protozoa were observed in the cecal and colonic mucosa and submucosa. Brachyspira hyodysenteriae was detected by PCR from colonic conten

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Dessì, Daniele, Giuseppe Delogu, Eleonora Emonte, Maria Rosaria Catania, Pier Luigi Fiori, and Paola Rappelli. "Long-Term Survival and Intracellular Replication of Mycoplasma hominis in Trichomonas vaginalis Cells: Potential Role of the Protozoon in Transmitting Bacterial Infection." Infection and Immunity 73, no. 2 (2005): 1180–86. http://dx.doi.org/10.1128/iai.73.2.1180-1186.2005.

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ABSTRACT The existence of a symbiotic relationship between Trichomonas vaginalis and Mycoplasma hominis, which is the first reported example of symbiosis between two obligate human pathogens, has been recently reported by our research group. In this work, we examined the cellular location of M. hominis in respect to T. vaginalis. By using gentamicin protection assays, double immunofluorescence, and confocal microscopy, we obtained strong evidence that M. hominis is located within protozoan cells. 5-Bromodeoxyuridine incorporation assays showed that intracellularly located mycoplasmas actively

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Ceplecha, V., V. Svobodova, C. Lendon, R. Husnik, K. Horackova, and M. Svoboda. "A survey of feline trichomonosis suggests a low incidence of Tritrichomonas blagburni among cats in the Czech Republic." Veterinární Medicína 62, No. 5 (2017): 269–73. http://dx.doi.org/10.17221/106/2016-vetmed.

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Tritrichomonas blagburni (previously called T. foetus) has been implicated as an aetiological agent of long-term large-bowel diarrhoea in cats in many countries worldwide. The aim of this study was to determine the presence of, and risk factors for T. blagburni among a cohort of cats living in different conditions in the Czech Republic. Samples were collected from 170 cats living in different environments. The InPouch™ TF-Feline medium method was used for diagnosis of feline trichomonosis. A single case (0.6%) with motile trichomonads identified as Pentatrichomonas hominis was found in a cat f

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Narcisi, E. M., and W. E. Secor. "In vitro effect of tinidazole and furazolidone on metronidazole-resistant Trichomonas vaginalis." Antimicrobial Agents and Chemotherapy 40, no. 5 (1996): 1121–25. http://dx.doi.org/10.1128/aac.40.5.1121.

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Trichomonas vaginalis is a common sexually transmitted protozoan parasite. Although often considered simply a nuisance infection, T. vaginalis has been implicated in premature rupture of placental membranes and increases in the risk of acquiring human immunodeficiency virus. Metronidazole, a 5-nitroimidazole, is currently the drug of choice to treat T. vaginalis infection. Because some patients have severe reactions to metronidazole and others are infected with metronidazole-resistant T. vaginalis, we were prompted to investigate alternative therapies. Tinidazole, another 5-nitroimidazole used

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