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1

Kirby, Richard R., and Gregory Beaugrand. "Trophic amplification of climate warming." Proceedings of the Royal Society B: Biological Sciences 276, no. 1676 (2009): 4095–103. http://dx.doi.org/10.1098/rspb.2009.1320.

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Ecosystems can alternate suddenly between contrasting persistent states due to internal processes or external drivers. It is important to understand the mechanisms by which these shifts occur, especially in exploited ecosystems. There have been several abrupt marine ecosystem shifts attributed either to fishing, recent climate change or a combination of these two drivers. We show that temperature has been an important driver of the trophodynamics of the North Sea, a heavily fished marine ecosystem, for nearly 50 years and that a recent pronounced change in temperature established a new ecosyst
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2

Chust, Guillem, J. Icarus Allen, Laurent Bopp, et al. "Biomass changes and trophic amplification of plankton in a warmer ocean." Global Change Biology 20, no. 7 (2014): 2124–39. https://doi.org/10.1111/gcb.12562.

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Ocean warming can modify the ecophysiology and distribution of marine organisms, and relationships between species, with nonlinear interactions between ecosystem components potentially resulting in trophic amplification. Trophic amplification (or attenuation) describe the propagation of a hydroclimatic signal up the food web, causing magnification (or depression) of biomass values along one or more trophic pathways. We have employed 3-D coupled physical-biogeochemical models to explore ecosystem responses to climate change with a focus on trophic amplification. The response of phytoplankton an
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3

Stock, C. A., J. P. Dunne, and J. G. John. "Drivers of trophic amplification of ocean productivity trends in a changing climate." Biogeosciences 11, no. 24 (2014): 7125–35. http://dx.doi.org/10.5194/bg-11-7125-2014.

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Abstract. Pronounced projected 21st century trends in regional oceanic net primary production (NPP) raise the prospect of significant redistributions of marine resources. Recent results further suggest that NPP changes may be amplified at higher trophic levels. Here, we elucidate the role of planktonic food web dynamics in driving projected changes in mesozooplankton production (MESOZP) found to be, on average, twice as large as projected changes in NPP by the latter half of the 21st century under a high emissions scenario in the Geophysical Fluid Dynamics Laboratory's ESM2M–COBALT (Carbon, Oc
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4

Stock, C. A., J. P. Dunne, and J. G. John. "Drivers of trophic amplification of ocean productivity trends in a changing climate." Biogeosciences Discussions 11, no. 7 (2014): 11331–59. http://dx.doi.org/10.5194/bgd-11-11331-2014.

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Abstract. Pronounced projected 21st century trends in regional oceanic net primary production (NPP) raise the prospect of significant redistributions of marine resources. Recent results further suggest that NPP changes may be amplified at higher trophic levels. Here, we elucidate the role of planktonic food web dynamics in driving projected changes in mesozooplankton production (MESOZP) found to be, on average, twice as large as projected changes in NPP by the latter half of the 21st century under a high emissions scenario. Globally, MESOZP was projected to decline by 7.9% but regional MESOZP
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5

Kwiatkowski, Lester, Olivier Aumont, and Laurent Bopp. "Consistent trophic amplification of marine biomass declines under climate change." Global Change Biology 25, no. 1 (2018): 218–29. http://dx.doi.org/10.1111/gcb.14468.

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6

Guibourd de Luzinais, Vianney, Hubert du Pontavice, Gabriel Reygondeau, et al. "Trophic amplification: A model intercomparison of climate driven changes in marine food webs." PLOS ONE 18, no. 8 (2023): e0287570. http://dx.doi.org/10.1371/journal.pone.0287570.

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Marine animal biomass is expected to decrease in the 21st century due to climate driven changes in ocean environmental conditions. Previous studies suggest that the magnitude of the decline in primary production on apex predators could be amplified through the trophodynamics of marine food webs, leading to larger decreases in the biomass of predators relative to the decrease in primary production, a mechanism called trophic amplification. We compared relative changes in producer and consumer biomass or production in the global ocean to assess the extent of trophic amplification. We used simula
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7

Chust, Guillem, J. Icarus Allen, Laurent Bopp, et al. "Biomass changes and trophic amplification of plankton in a warmer ocean." Global Change Biology 20, no. 7 (2014): 2124–39. http://dx.doi.org/10.1111/gcb.12562.

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8

Lotze, Heike K., Derek P. Tittensor, Andrea Bryndum-Buchholz, et al. "Global ensemble projections reveal trophic amplification of ocean biomass declines with climate change." Proceedings of the National Academy of Sciences 116, no. 26 (2019): 12907–12. http://dx.doi.org/10.1073/pnas.1900194116.

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While the physical dimensions of climate change are now routinely assessed through multimodel intercomparisons, projected impacts on the global ocean ecosystem generally rely on individual models with a specific set of assumptions. To address these single-model limitations, we present standardized ensemble projections from six global marine ecosystem models forced with two Earth system models and four emission scenarios with and without fishing. We derive average biomass trends and associated uncertainties across the marine food web. Without fishing, mean global animal biomass decreased by 5%
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9

Lindley, J. A., G. Beaugrand, C. Luczak, J. M. Dewarumez, and R. R. Kirby. "Warm-water decapods and the trophic amplification of climate in the North Sea." Biology Letters 6, no. 6 (2010): 773–76. http://dx.doi.org/10.1098/rsbl.2010.0394.

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A long-term time series of plankton and benthic records in the North Sea indicates an increase in decapods and a decline in their prey species that include bivalves and flatfish recruits. Here, we show that in the southern North Sea the proportion of decapods to bivalves doubled following a temperature-driven, abrupt ecosystem shift during the 1980s. Analysis of decapod larvae in the plankton reveals a greater presence and spatial extent of warm-water species where the increase in decapods is greatest. These changes paralleled the arrival of new species such as the warm-water swimming crab Pol
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10

Lepère, Cécile, Isabelle Domaizon, and Didier Debroas. "Unexpected Importance of Potential Parasites in the Composition of the Freshwater Small-Eukaryote Community." Applied and Environmental Microbiology 74, no. 10 (2008): 2940–49. http://dx.doi.org/10.1128/aem.01156-07.

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ABSTRACT The diversity of small eukaryotes (0.2 to 5 μm) in a mesotrophic lake (Lake Bourget) was investigated using 18S rRNA gene library construction and fluorescent in situ hybridization coupled with tyramide signal amplification (TSA-FISH). Samples collected from the epilimnion on two dates were used to extend a data set previously obtained using similar approaches for lakes with a range of trophic types. A high level of diversity was recorded for this system with intermediate trophic status, and the main sequences from Lake Bourget were affiliated with ciliates (maximum, 19% of the operat
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11

Pedersen, Karen M., Christoph von Beeren, Arianna Oggioni, and Nico Blüthgen. "Mammal dung–dung beetle trophic networks: an improved method based on gut-content DNA." PeerJ 12 (March 15, 2024): e16627. http://dx.doi.org/10.7717/peerj.16627.

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Background Dung beetles provide many important ecosystem services, including dung decomposition, pathogen control, soil aeration, and secondary seed dispersal. Yet, the biology of most dung beetles remains unknown. Natural diets are poorly studied, partly because previous research has focused on choice or attraction experiments using few, easily accessible dung types from zoo animals, farm animals, or humans. This way, many links within natural food webs have certainly been missed. In this work, we aimed to establish a protocol to analyze the natural diets of dung beetles using DNA gut barcodi
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12

Besedinа, E. N., and V. I. Kil. "Genetic variability of the pear lace bug Stephanitis pyri F. (Heteroptera: Tingidae) in Krasnodar Territory, Russia." South of Russia: ecology, development 18, no. 3 (2023): 28–38. http://dx.doi.org/10.18470/1992-1098-2023-3-28-38.

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Aim. Study of the genetic variability of the pear lace bug Stephanitis pyri F. depending on geographical location and trophic specialization.Material and Methods. The object of the study was insect samples (n=60) from the natural population of the pear lace bug Stephanitis pyri F. (Tingidae: Heteroptera) in the Krasnodar Territory: in Krasnodar, Kropotkin and Novorossiysk. The collection of S. pyri bugs was carried out on model trees of domestic apple (Malus domestica Borkh) and sour cherry (Prunus cerasus L.). Molecular genetic analysis was carried out in several stages: sample preparation, D
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13

Chai, Yanchao, Xin Wang, Haiqing Wang, Yu Zhang, Zhongqi Dai, and Jiaxin Yang. "Tire wear particle leachate exhibits trophic and multi-generational amplification: Potential threat to population viability." Journal of Hazardous Materials 480 (December 2024): 136497. http://dx.doi.org/10.1016/j.jhazmat.2024.136497.

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14

Jatmiko, Irwan, Raymon Rahmanov Zedta, Maya Agustina, and Bram Setyadji. "Genetic Diversity and Demography of Skipjack Tuna (Katsuwonus pelamis) In Southern and Western Part of Indonesian Waters." ILMU KELAUTAN: Indonesian Journal of Marine Sciences 24, no. 2 (2019): 61. http://dx.doi.org/10.14710/ik.ijms.24.2.61-68.

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Skipjack tuna (Katsuwonus pelamis) is highly migratory species that spread from trophic and sub trophic waters. This species can be found in Atlantic, Indian and Pacific oceans. The genetic information of highly migratory species like skipjack tuna is important to support the sustainability of the fisheries. The objectives of this study are to gain information genetic diversity and population structure of exploited species and to understand the population kinship in Indonesian waters. Tissue samples were collected from six locations, i.e.: Sibolga (North Sumatera), Padang (West Sumatera), Binu
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15

Van Looy, Kris, Mathieu Floury, Martial Ferréol, Marta Prieto-Montes, and Yves Souchon. "Long-term changes in temperate stream invertebrate communities reveal a synchronous trophic amplification at the turn of the millennium." Science of The Total Environment 565 (September 2016): 481–88. http://dx.doi.org/10.1016/j.scitotenv.2016.04.193.

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16

Browett, Samuel S., Thomas G. Curran, Denise B. O’Meara, et al. "Primer biases in the molecular assessment of diet in multiple insectivorous mammals." Mammalian Biology 101, no. 3 (2021): 293–304. http://dx.doi.org/10.1007/s42991-021-00115-4.

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AbstractOur understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here, we apply DNA metabarcoding to empirically compare the prey detection capabilities of two widely used primer sets when assessing the
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17

POULIN, ROBERT, and CLÉMENT LAGRUE. "The ups and downs of life: population expansion and bottlenecks of helminth parasites through their complex life cycle." Parasitology 142, no. 6 (2015): 791–99. http://dx.doi.org/10.1017/s0031182014001917.

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SUMMARYThe fundamental assumption underpinning the evolution of numerous adaptations shown by parasites with complex life cycles is that huge losses are incurred by infective stages during certain transmission steps. However, the magnitude of transmission losses or changes in the standing crop of parasites passing from upstream (source) to downstream (target) hosts have never been quantified in nature. Here, using data from 100 pairs of successive upstream–downstream life stages, from distinct populations representing 10 parasite species, we calculated the total density per m2 of successive li
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18

Hunter, R. H. F., and N. Einer-Jensen. "Potential amplification of early pregnancy signals by ovarian follicular cells in suspension within the Fallopian tube." Zygote 11, no. 3 (2003): 237–43. http://dx.doi.org/10.1017/s0967199403002284.

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That a mammalian embryo might influence the maternal endocrinological and/or immunological systems whilst still in the Fallopian tube has long seemed possible (Fig. 1). Under normal circumstances, the embryo contains a full diploid complement of genetic information and thus the point at issue is just how soon instructions in the genome are expressed for trophic purposes. It could be argued from several points of view that it would be biologically prudent for an embryo to influence the mother whilst still at an early stage of development. Consideration of the extremely intimate apposition of th
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19

Bogani, Patrizia, Alessandra Simoni, Priscilla Bettini, Maria Mugnai, M. Gabriella Pellegrini, and Marcello Buiatti. "Genome flux in tomato auto- and auxo-trophic cell clones cultured in different auxin/cytokinin equilibria. I. DNA multiplicity and methylation levels." Genome 38, no. 5 (1995): 902–12. http://dx.doi.org/10.1139/g95-119.

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An analysis of the effect of changing physiological conditions on genetic stability, in terms of epigenetic changes, such as DNA, methylation patterns, and multiplicity of repetitive DNA, was carried out on tomato cell clones grown on media supplemented with different auxin/cytokinin ratios. The effect of endogenous variation in phytohormone equilibria was also indirectly analysed through a comparison of auxotrophic or habituated (autotrophic) cell clones and the differentiated leaf tissue. The data obtained showed significant variation in methylation and multiplicity levels both between clone
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20

Browett, Samuel S., Thomas G. Curran, Denise B. O'Meara, et al. "Primer biases in the molecular assessment of diet in multiple insectivorous mammals." Mammalian Biology 101, no. 3 (2021): 293–304. https://doi.org/10.5281/zenodo.13480841.

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(Uploaded by Plazi for the Bat Literature Project) Our understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here, we apply DNA metabarcoding to empirically compare the prey detection capabilities of two
APA, Harvard, Vancouver, ISO, and other styles
21

Browett, Samuel S., Thomas G. Curran, Denise B. O'Meara, et al. "Primer biases in the molecular assessment of diet in multiple insectivorous mammals." Mammalian Biology 101, no. 3 (2021): 293–304. https://doi.org/10.5281/zenodo.13480841.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) Our understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here, we apply DNA metabarcoding to empirically compare the prey detection capabilities of two
APA, Harvard, Vancouver, ISO, and other styles
22

Browett, Samuel S., Thomas G. Curran, Denise B. O'Meara, et al. "Primer biases in the molecular assessment of diet in multiple insectivorous mammals." Mammalian Biology 101, no. 3 (2021): 293–304. https://doi.org/10.5281/zenodo.13480841.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) Our understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here, we apply DNA metabarcoding to empirically compare the prey detection capabilities of two
APA, Harvard, Vancouver, ISO, and other styles
23

Browett, Samuel S., Thomas G. Curran, Denise B. O'Meara, et al. "Primer biases in the molecular assessment of diet in multiple insectivorous mammals." Mammalian Biology 101, no. 3 (2021): 293–304. https://doi.org/10.5281/zenodo.13480841.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) Our understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here, we apply DNA metabarcoding to empirically compare the prey detection capabilities of two
APA, Harvard, Vancouver, ISO, and other styles
24

Browett, Samuel S., Thomas G. Curran, Denise B. O'Meara, et al. "Primer biases in the molecular assessment of diet in multiple insectivorous mammals." Mammalian Biology 101, no. 3 (2021): 293–304. https://doi.org/10.5281/zenodo.13480841.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) Our understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here, we apply DNA metabarcoding to empirically compare the prey detection capabilities of two
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25

Landry, MR, RR Hood, and CH Davies. "Mesozooplankton biomass and temperature-enhanced grazing along a 110°E transect in the eastern Indian Ocean." Marine Ecology Progress Series 649 (September 10, 2020): 1–19. http://dx.doi.org/10.3354/meps13444.

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Low-latitude waters of the Indian Ocean are warming faster than other major oceans. Most models predict a zooplankton decline due to lower productivity, enhanced metabolism and phytoplankton size shifts that reduce trophic transfer efficiency. In May-June 2019, we investigated mesozooplankton biomass and grazing along the historic 110°E transect line from the International Indian Ocean Expedition (IIOE) of the 1960s. Twenty sampling stations from 39.5 to 11.5°S spanned latitudinal variability from temperate to tropical waters and a pronounced 14°C gradient in mean euphotic zone temperature. Al
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26

Ávila-Torres, Gerardo, Gabriela Rosiles-González, Victor Hugo Carrillo-Jovel, et al. "Microcystin Concentrations and Detection of the mcyA Gene in Water Collected from Agricultural, Urban, and Recreational Areas in a Karst Aquifer in the Yucatan Peninsula of Mexico." Microbiology Research 14, no. 3 (2023): 1168–84. http://dx.doi.org/10.3390/microbiolres14030078.

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The eutrophication of freshwater ecosystems allows the proliferation of cyanobacteria that can produce secondary metabolites such as microcystins. The main aim of this study was to explore the occurrence and concentration of microcystin and the mcyA gene in water bodies located in agricultural, urban, and recreational areas in the karst aquifer of the Yucatan peninsula of Mexico (YPM) and to analyze the water quality variables and chlorophyll-a (Chl-a) associated with their presence. Water samples were collected from 14 sites, and microcystin concentrations were quantified using antibody-based
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27

Judd, Miranda, Jens Wira, Allen R. Place, and Tsvetan Bachvaroff. "Long-Read Sequencing Unlocks New Insights into the Amphidinium carterae Microbiome." Marine Drugs 22, no. 8 (2024): 342. http://dx.doi.org/10.3390/md22080342.

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Dinoflagellates are one of the largest groups of marine microalgae and exhibit diverse trophic strategies. Some dinoflagellates can produce secondary metabolites that are known to be toxic, which can lead to ecologically harmful blooms. Amphidinium carterae is one species of dinoflagellate that produces toxic compounds and is used as a model for dinoflagellate studies. The impact of the microbiome on A. carterae growth and metabolite synthesis is not yet fully understood, nor is the impact of bacterial data on sequencing and assembly. An antibiotic cocktail was previously shown to eliminate 16
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28

Fablet, Laura, Anouk Pellerin, Diane Zarzoso-Lacoste, Vincent Dubut, and Laurence Walch. "Metabarcoding reveals waterbird diet in a French Ramsar wetland: implications for ecosystem management." Knowledge & Management of Aquatic Ecosystems, no. 425 (2024): 9. http://dx.doi.org/10.1051/kmae/2024005.

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Environmental and/or climate changes, occurring at a global or local scale, can significantly impact the diets, health, and population dynamics of waterbirds. This study aimed to develop an effective tool, using DNA metabarcoding of fecal samples, for monitoring waterbird diets during the breeding season in a Ramsar freshwater wetland in Northern France. We collected bird feces across eight marshes with varying anthropic usage. The majority of samples (69%) were from five waterbird species: Eurasian coot (Fulica atra), Eurasian moorhen (Gallinula chloropus), mallard (Anas platyrhynchos), mute
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29

Ibáñez, C., D. Fukui, A. .G. Popa‐Lisseanu, D. Pastor‐Beviá, J. L. García‐Mudarra, and J. Juste. "Molecular identification of bird species in the diet of the bird‐like noctule bat in Japan." Journal of Zoology 313, no. 4 (2021): 276–82. https://doi.org/10.5281/zenodo.13449495.

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(Uploaded by Plazi for the Bat Literature Project) We investigated the birds in the diet of the bird-like noctule (Nyctalus aviator) through DNA amplification from feather remains found in faecal pellets. Our goal was to confirm whether N. aviator preys on nocturnally migratory species, as does its European relative N. lasiopterus, and to gain insight into this hunting strategy (e.g. on the wing vs. from cavities). The diversity and the characteristics of the birds found in the faecal remains indicate that in Japan N. aviator employs a similar hunting strategy on songbirds as N. lasiopterus in
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30

Ibáñez, C., D. Fukui, A. .G. Popa‐Lisseanu, D. Pastor‐Beviá, J. L. García‐Mudarra, and J. Juste. "Molecular identification of bird species in the diet of the bird‐like noctule bat in Japan." Journal of Zoology 313, no. 4 (2021): 276–82. https://doi.org/10.5281/zenodo.13449495.

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(Uploaded by Plazi for the Bat Literature Project) We investigated the birds in the diet of the bird-like noctule (Nyctalus aviator) through DNA amplification from feather remains found in faecal pellets. Our goal was to confirm whether N. aviator preys on nocturnally migratory species, as does its European relative N. lasiopterus, and to gain insight into this hunting strategy (e.g. on the wing vs. from cavities). The diversity and the characteristics of the birds found in the faecal remains indicate that in Japan N. aviator employs a similar hunting strategy on songbirds as N. lasiopterus in
APA, Harvard, Vancouver, ISO, and other styles
31

Ibáñez, C., D. Fukui, A. .G. Popa‐Lisseanu, D. Pastor‐Beviá, J. L. García‐Mudarra, and J. Juste. "Molecular identification of bird species in the diet of the bird‐like noctule bat in Japan." Journal of Zoology 313, no. 4 (2021): 276–82. https://doi.org/10.5281/zenodo.13449495.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) We investigated the birds in the diet of the bird-like noctule (Nyctalus aviator) through DNA amplification from feather remains found in faecal pellets. Our goal was to confirm whether N. aviator preys on nocturnally migratory species, as does its European relative N. lasiopterus, and to gain insight into this hunting strategy (e.g. on the wing vs. from cavities). The diversity and the characteristics of the birds found in the faecal remains indicate that in Japan N. aviator employs a similar hunting strategy on songbirds as N. lasiopterus in
APA, Harvard, Vancouver, ISO, and other styles
32

Ibáñez, C., D. Fukui, A. .G. Popa‐Lisseanu, D. Pastor‐Beviá, J. L. García‐Mudarra, and J. Juste. "Molecular identification of bird species in the diet of the bird‐like noctule bat in Japan." Journal of Zoology 313, no. 4 (2021): 276–82. https://doi.org/10.5281/zenodo.13449495.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) We investigated the birds in the diet of the bird-like noctule (Nyctalus aviator) through DNA amplification from feather remains found in faecal pellets. Our goal was to confirm whether N. aviator preys on nocturnally migratory species, as does its European relative N. lasiopterus, and to gain insight into this hunting strategy (e.g. on the wing vs. from cavities). The diversity and the characteristics of the birds found in the faecal remains indicate that in Japan N. aviator employs a similar hunting strategy on songbirds as N. lasiopterus in
APA, Harvard, Vancouver, ISO, and other styles
33

Ibáñez, C., D. Fukui, A. .G. Popa‐Lisseanu, D. Pastor‐Beviá, J. L. García‐Mudarra, and J. Juste. "Molecular identification of bird species in the diet of the bird‐like noctule bat in Japan." Journal of Zoology 313, no. 4 (2021): 276–82. https://doi.org/10.5281/zenodo.13449495.

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Abstract:
(Uploaded by Plazi for the Bat Literature Project) We investigated the birds in the diet of the bird-like noctule (Nyctalus aviator) through DNA amplification from feather remains found in faecal pellets. Our goal was to confirm whether N. aviator preys on nocturnally migratory species, as does its European relative N. lasiopterus, and to gain insight into this hunting strategy (e.g. on the wing vs. from cavities). The diversity and the characteristics of the birds found in the faecal remains indicate that in Japan N. aviator employs a similar hunting strategy on songbirds as N. lasiopterus in
APA, Harvard, Vancouver, ISO, and other styles
34

Ascher, Alexander, Peter D. Countway, Robin S. Sleith, et al. "Contemporary eDNA methods complement conventional microscopy in zooplankton diet studies: Case study with American lobster postlarvae." PLOS One 20, no. 6 (2025): e0325889. https://doi.org/10.1371/journal.pone.0325889.

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The diets of pelagic marine larvae are difficult to analyze due to their small size and even smaller prey. Furthermore, different methods may lead to alternative interpretations of trophic interactions. Conventionally, diet studies have relied primarily on visual identification of prey through dissection and microscopy. While microscopy has clear benefits, it can yield an incomplete assessment of diet since smaller and soft-bodied prey items are often difficult to identify. Here, we combined conventional microscopy and two contemporary environmental DNA (eDNA) methods: DNA metabarcode sequenci
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35

Bogani, Patrizia, Alessandra Simoni, Pietro Lio', Angela Germinario, and Marcello Buiatti. "Molecular variation in plant cell populations evolving in vitro in different physiological contexts." Genome 44, no. 4 (2001): 549–58. http://dx.doi.org/10.1139/g01-033.

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Previous work has shown the fixation of context-specific random amplified polymorphic DNA (RAPD) patterns in tomato cell cultures grown for 2 years in different hormonal contexts. In this work, RAPD sequences were characterised and RAPD-derived molecular markers used for a further study of variation between and within auto- and auxo-trophic tomato cultures grown in different hormonal equilibria. Results were then compared with those obtained using microsatellite markers located in noncoding regions of differentiation- and hormone-related genes and with those obtained with the external transcri
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36

Kenis, Vladimir M. "Amplified pain and complex regional pain syndrome in children: clinical cases and literature review." Pediatric Traumatology, Orthopaedics and Reconstructive Surgery 12, no. 3 (2024): 361–76. http://dx.doi.org/10.17816/ptors634985.

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BACKGROUND: Musculoskeletal pain is a common reason for visiting pediatric clinics. Causes of chronic musculoskeletal pain include various inflammatory or noninflammatory conditions. Amplified pain syndrome refers to a wide range of conditions manifested by chronic pain, the common feature of which is central and/or peripheral sensory amplification of pain. Complex regional pain syndrome is characterized by spontaneously occurring or provoked by irritating stimuli pain of high intensity, disproportionate to the actual injury or other stimulus, and the presence of several concomitant vegetative
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Pumariño, L., O. Alomar, and N. Agustí. "Development of specific ITS markers for plant DNA identification within herbivorous insects." Bulletin of Entomological Research 101, no. 3 (2010): 271–76. http://dx.doi.org/10.1017/s0007485310000465.

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AbstractDNA-based techniques have proved to be very useful methods to study trophic relationships between pests and their natural enemies. However, most predators are best defined as omnivores, and the identification of plant-specific DNA should also allow the identification of the plant species the predators have been feeding on. In this study, a PCR approach based on the development of specific primers was developed as a self-marking technique to detect plant DNA within the gut of one heteropteran omnivorous predator (Macrolophus pygmaeus) and two lepidopteran pest species (Helicoverpa armig
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Menconi, Vasco, Perla Tedesco, Paolo Pastorino, et al. "Could Fish Feeding Behaviour and Size Explain Prevalence Differences of the Nematode Eustrongylides excisus among Species? The Case Study of Lake Garda." Water 13, no. 24 (2021): 3581. http://dx.doi.org/10.3390/w13243581.

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The nematode Eustrongylides excisus is a parasite of freshwater fish- and fish-eating birds, with known differences on prevalence values among fish species. Thus, the present study aims to explore the hypothesis that the feeding behavior and the size of fish belonging to different trophic levels could explain such differences. For that, 14 sampling sites were selected to perform a fish parasitological survey on Lake Garda (Italy) during spring-summer 2020. Amplification of nuclear ribosomal internal transcribed spacer (ITS) rDNA sequences of nematodes morphologically ascribable to the genus Eu
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Barton, AD, F. González Taboada, A. Atkinson, CE Widdicombe, and CA Stock. "Integration of temporal environmental variation by the marine plankton community." Marine Ecology Progress Series 647 (August 13, 2020): 1–16. http://dx.doi.org/10.3354/meps13432.

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Theory and observations suggest that low frequency variation in marine plankton populations, or red noise, may arise through cumulative integration of white noise atmospheric forcing by the ocean and its amplification within food webs. Here, we revisit evidence for the integration of stochastic atmospheric variations by comparing the power spectra of time series of atmospheric and oceanographic conditions to the population dynamics of 150 plankton taxa at Station L4 in the Western English Channel. The power spectra of oceanographic conditions (sea surface temperature, surface nitrate) are redd
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Traugott, M., and W. O. C. Symondson. "Molecular analysis of predation on parasitized hosts." Bulletin of Entomological Research 98, no. 3 (2008): 223–31. http://dx.doi.org/10.1017/s0007485308005968.

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AbstractPredation on parasitized hosts can significantly affect natural enemy communities, and such intraguild predation may indirectly affect control of herbivore populations. However, the methodological challenges for studying these often complex trophic interactions are formidable. Here, we evaluate a DNA-based approach to track parasitism and predation on parasitized hosts in model herbivore-parasitoid-predator systems. Using singleplex polymerase chain reaction (SP-PCR) to target mtDNA of the parasitoid only, and multiplex PCR (MP-PCR) to additionally target host DNA as an internal amplif
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DIOMEDE, Luisa, Silvano SOZZANI, Walter LUINI, et al. "Activation effects of a prion protein fragment [PrP-(106-126)] on human leucocytes*." Biochemical Journal 320, no. 2 (1996): 563–70. http://dx.doi.org/10.1042/bj3200563.

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Prion-related encephalopathies are characterized by the intracerebral accumulation of an abnormal isoform of the cellular prion protein (PrPC) named scrapie prion protein (PrPSc). The pathological forms of this protein and its cellular precursor are not only expressed in the brain but also, at lower concentrations, in peripheral tissues. We recently showed that a synthetic peptide corresponding to residues 106–126 [PrP-(106–126)] of the human PrP is toxic to neurons and trophic to astrocytes in vitro. Our experiments were aimed at verifying whether PrP-(106–126) and other peptides correspondin
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Aristizábal-Duque, Sandra L., Tatiana Diosa-Mejía, Carolina Zapata-Escobar, and Luz Y. Orozco-Jiménez. "Molecular sexing and sex ratio of the neotropical otter (Lontra longicaudis annectens) using non-invasive samples from the Porce III Reservoir (Antioquia, Colombia)." Therya 16, no. 2 (2025): 305–12. https://doi.org/10.12933/therya-25-6172.

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The Neotropical otter is an important mammal in aquatic ecosystems due to its trophic position as a top predator, therefore is a valuable medium and long term bio monitor of the environment. Due to its crepuscular-nocturnal behavior, studies on this species primarily rely on non invasive methods, such as the analysis of feces and anal-glands jellies, which allow for various ecological and population inferences. With recent advances in molecular techniques, these non-invasive samples have greatly contributed to the understanding of wild populations. Among the relevant population characteristics
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Mavingui, Patrick, Toon Laeremans, Margarita Flores, David Romero, Esperanza Martínez-Romero, and Rafael Palacios. "Genes Essential for Nod Factor Production and Nodulation Are Located on a Symbiotic Amplicon (AMPRtrCFN299pc60) in Rhizobium tropici." Journal of Bacteriology 180, no. 11 (1998): 2866–74. http://dx.doi.org/10.1128/jb.180.11.2866-2874.1998.

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ABSTRACT Amplifiable DNA regions (amplicons) have been identified in the genome of Rhizobium etli. Here we report the isolation and molecular characterization of a symbiotic amplicon of Rhizobium tropici. To search for symbiotic amplicons, a cartridge containing a kanamycin resistance marker that responds to gene dosage and conditional origins of replication and transfer was inserted in the nodulation region of the symbiotic plasmid (pSym) of R. tropici CFN299. Derivatives harboring amplifications were selected by increasing the concentration of kanamycin in the cell culture. The amplified DNA
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Moldovan, Rebeca, Bogdan-Cezar Iacob, Cosmin Farcău, Ede Bodoki, and Radu Oprean. "Strategies for SERS Detection of Organochlorine Pesticides." Nanomaterials 11, no. 2 (2021): 304. http://dx.doi.org/10.3390/nano11020304.

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Organochlorine pesticides (OCPs) embody highly lipophilic hazardous chemicals that are being phased out globally. Due to their persistent nature, they are still contaminating the environment, being classified as persistent organic pollutants (POPs). They bioaccumulate through bioconcentration and biomagnification, leading to elevated concentrations at higher trophic levels. Studies show that human long-term exposure to OCPs is correlated with a large panel of common chronic diseases. Due to toxicity concerns, most OCPs are listed as persistent organic pollutants (POPs). Conventionally, separat
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Ducic, Vladan, Bosko Milovanovic, Gorica Stanojevic, Milan Milenkovic, and Nina Curcic. "Tropical temperature altitude amplification in the hiatus period (1998-2012)." Thermal Science 19, suppl. 2 (2015): 371–79. http://dx.doi.org/10.2298/tsci150410103d.

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In the period 1998-2012 there was a stagnation in temperature rise, despite the GHGs radiation forcing is increased (hiatus period). According to Global Circulation Models simulations, expected response on the rise of GHGs forcing is tropical temperature altitude amplification - temperature increases faster in higher troposphere than in lower troposphere. In this paper, two satellite data sets, UAH MSU and RSS, were used to test altitude temperature amplification in tropic (20?N-20?S) in the hiatus period. We compared data from satellite data sets from lower troposphere (TLT) and middle tropos
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Cai, Ming, and Ka-Kit Tung. "Robustness of Dynamical Feedbacks from Radiative Forcing: 2% Solar versus 2 × CO2 Experiments in an Idealized GCM." Journal of the Atmospheric Sciences 69, no. 7 (2012): 2256–71. http://dx.doi.org/10.1175/jas-d-11-0117.1.

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Abstract Despite the differences in the spatial patterns of the external forcing associated with a doubling CO2 and with a 2% solar variability, the final responses in the troposphere and at the surface in a three-dimensional general circulation model appear remarkably similar. Various feedback processes are diagnosed and compared using the climate feedback–response analysis method (CFRAM) to understand the mechanisms responsible. At the surface, solar radiative forcing is stronger in the tropics than at the high latitudes, whereas greenhouse radiative forcing is stronger at high latitudes com
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Alraey, Yasser, Rasha Alhweti, Hatim Almutairi, et al. "Molecular Characterization of Leishmania Species among Patients with Cutaneous Leishmaniasis in Asir Province, Saudi Arabia." Pathogens 11, no. 12 (2022): 1472. http://dx.doi.org/10.3390/pathogens11121472.

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Anthroponotic cutaneous leishmaniais (ACL) and zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania tropica and Leishmania major, respectively, are endemic vector-borne diseases in southern Saudi Arabia. In 2021, an outbreak of cutaneous leishmaniasis occurred in the province of Asir. The main objective of our investigation was to analyze the epidemiological features of CL in southern Saudi Arabia. The ministry of health recorded 194 CL patients between January and December 2021 from the Asir province. Our findings showed that the majority of CL patients (87.1%) originated from the gove
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Kim, Jeongmin, Andrés Álvarez-Rodríguez, Zeng Li, Magdalena Radwanska, and Stefan Magez. "Recent Progress in the Detection of Surra, a Neglected Disease Caused by Trypanosoma evansi with a One Health Impact in Large Parts of the Tropic and Sub-Tropic World." Microorganisms 12, no. 1 (2023): 44. http://dx.doi.org/10.3390/microorganisms12010044.

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Surra is a wasting disease triggered by infection with Trypanosoma evansi, a protozoan blood parasite that causes mortality and morbidity in a broad spectrum of wild and domestic animals and occasionally humans. Trypanosoma evansi has the widest geographical spread among all pathogenic trypanosomes, inflicting significant worldwide economic problems due to its adverse effects on meat and milk production. For diagnosis, most endemic countries continue to rely on traditional parasitological and serological techniques, such as the analysis of blood smears by microscopy and the Card Agglutination
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Pitz, Kathleen, Collin Closek, Anni Djurhuus, et al. "Rewards and Challenges of eDNA Sequencing with Multiple Genetic Markers for Marine Observation Programs." Proceedings of TDWG 1 (August 24, 2017): e20548. https://doi.org/10.3897/tdwgproceedings.1.20548.

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Metabarcoding of environmental DNA (eDNA) samples holds new promise to increase our ability to measure changes in biodiversity and community composition over time. It can allow the characterization of large groups of organisms where traditional sampling may be impractical or not cost-effective. However, it is still unclear how best to compare and combine this information with morphological counts in order to inform policies and biodiversity metrics that are based on traditional sampling results. Under the Marine Biodiversity Observation Network (MBON) initiative, multiple taxonomic marker gene
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Kimno, Lydia Chepkoech, Javan Omondi Were, Isaac Kofi Bimpong, Miriam Gaceri Kinyua, and Stephen Kimno. "Virulence and Molecular Detection of Cassava Mosaic and Brown Streak Virus Genes on Elite Mutant Cassava Lines." Biotechnology Journal International 27, no. 6 (2023): 1–11. http://dx.doi.org/10.9734/bji/2023/v27i6701.

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Unavailability of germplasm with Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) tolerance genes remains the main bottleneck towards management of the two diseases in tropic and sub-tropic regions in Africa. We assessed the pathogenicity of two viruses and established the presence of genes linked to CBSD and CMD among some elite cassava lines using field techniques and PCR methods. The screen-house experiment was arranged in split plot in completely randomized block design replicated thrice. Severity data was done on a scale of 1 - 5 at an interval of 14 days post inoculat
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