Log in

Relevant bibliographies by topics / Trypan blue dye exclusion assay

Contents

Journal articles
Book chapters
Conference papers
Reports

Academic literature on the topic 'Trypan blue dye exclusion assay'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Trypan blue dye exclusion assay.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Trypan blue dye exclusion assay"

1

Bussadori, Sandra Kalil, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, et al. "Cytotoxicity assessment of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste." ConScientiae Saúde 9, no. 3 (2010): 354–59. http://dx.doi.org/10.5585/conssaude.v9i3.2224.

Full text

Abstract:

Introduction: Casein phosphopeptides (CPP) have been shown to be good carriers of calcium, phosphate, and hydroxide ions to promote enamel remineralization with applications in oral care products, professional dental products, and food products. Objectives: Evaluate the cytotoxicity of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste in rat fibroblasts. Materials and methods: Cytotoxicity was measured by the Trypan blue dye exclusion assay and the MTT assay. Results: Long term (1, 3, 5, and 7 days) and short term tests (0, 4, 8, and 12 hours) were performed with the Trypan b

APA, Harvard, Vancouver, ISO, and other styles

2

Raajshree.r, Khoushika, and Brindha Durairaj. "IN VITRO ANTICANCER POTENTIAL OF BIOSYNTHESIZED ZINC OXIDE NANOPARTICLES FROM THE SEAWEED TURBINARIA CONOIDES." Asian Journal of Pharmaceutical and Clinical Research 11, no. 5 (2018): 127. http://dx.doi.org/10.22159/ajpcr.2018.v11i5.22224.

Full text

Abstract:

Objective: The objective of this study is to investigate the anticancer potential of zinc oxide nanoparticles (ZnO-NPs) and hydroethanolic extract of Turbinaria conoides (HETC) against Dalton’s lymphoma ascites (DLA) cell line. Methods: Nanoparticles were synthesized from the HETC. An ultraviolet-visible spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology, and elemental composition of ZnO-NPs were also analyzed using scanning electron microscope-energy dispersive X-ray diffraction. The cytotoxic activity of ZnO-NPs and HETC was evaluated by 3-(4,5-di

APA, Harvard, Vancouver, ISO, and other styles

3

Vega, Maria V., Samuel K. Maheswaran, Joel R. Leininger, and Trevor R. Ames. "Adaptation of a colorimetric microtitration assay for quantifying Pasteurella haemolytica A1 leukotoxin and antileukotoxin." American Journal of Veterinary Research 48, no. 11 (1987): 1559–64. https://doi.org/10.2460/ajvr.1987.48.11.1559.

Full text

Abstract:

SUMMARY A colorimetric microtitration assay was adapted to quantify the cytotoxicity of Pasteurella haemolytica A1 leukotoxin to bovine neutrophils used as target cells. The viability of leukotoxin-treated target cells was detected by use of a tetrazolium dye that living cells reduced to dark blue formazan. The amount of formazan formed (which was quantified by use of an elisa plate reader) was directly proportional to the number of viable target cells. This assay system also was used to measure leu-kotoxin-neutralization antibody titers of bovine serum and lung lavage specimens obtained durin

APA, Harvard, Vancouver, ISO, and other styles

4

Et al., Hussein. "Trypan Blue Exclusion Assay Verifies in Vitro Cytotoxicity of New Cis-Platinum (II) Complex in Human Cells." Baghdad Science Journal 16, no. 3 (2019): 0555. http://dx.doi.org/10.21123/bsj.2019.16.3.0555.

Full text

Abstract:

Various assays are used to determine the toxic effects of drugs at cellular levels in vitro. One of these methods is the dye exclusion assay, which measures membrane integrity in the presence of Trypan blue. Trypan blue the dye which was used in this study to investigate cytotoxic effect of a new Cis –dichloroplatinum (II) complex [(Qu)2PtCl2] on the viability of polymorphonuclear cells (PMNs). Three concentrations of platinum complex were prepared (70, 35and 17.5 µg/ ml) and the results revealed that the percentage of cell viability decreased as the platinum complex concentration increased in

APA, Harvard, Vancouver, ISO, and other styles

5

Monte Neto, Rubens L., M. A. Sousa, Celidarque S. Dias, José M. Barbosa Filho, and Márcia R. Oliveira. "Yangambin Cytotoxicity: A Pharmacologically Active Lignan Obtained from Ocotea duckei Vattimo (Lauraceae)." Zeitschrift für Naturforschung C 63, no. 9-10 (2008): 681–86. http://dx.doi.org/10.1515/znc-2008-9-1012.

Full text

Abstract:

The in vitro cytotoxic potential of yangambin was evaluated. Yangambin is a pharmacologically active furofuran lignan obtained from the leaves of Ocotea duckei. It is the major compound from the lignoids fraction. Yangambin presented low cytotoxicity in all in vitro models analyzed. Its cytotoxicity to murine macrophages was measured by the Trypan blue dye exclusion test and MTT reduction assay, resulting in high CC50 values of 187.0 μg/mL (383.3 μm) and 246.7 μg/mL (504.3 μm), respectively. The difference obtained in the inhibitory concentrations aforementioned can be explained, at least in p

APA, Harvard, Vancouver, ISO, and other styles

6

Ahmad, Shama, Aftab Ahmad, B. Kelly Schneider, and Carl W. White. "Cholesterol Interferes with the MTT Assay in Human Epithelial-Like (A549) and Endothelial (HLMVE and HCAE) Cells." International Journal of Toxicology 25, no. 1 (2006): 17–23. http://dx.doi.org/10.1080/10915810500488361.

Full text

Abstract:

Metabolically active cells are able to convert the MTT [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide] dye to blue formazan. This is the basis of the MTT assay, which is among the most widely used screening methods to evaluate cell viability and proliferation. When testing the effects of cholesterol products on the viability of human pulmonary epithelial-like A549 cells using trypan blue staining (cell numbers) and the MTT assay, results were inconsistent. The MTT assay indicated greater than 50% loss of viability with exposure of cells to cholesterol, whereas there was no decrea

APA, Harvard, Vancouver, ISO, and other styles

7

Aloysius, Morris Terfa, Eleni Andreou, Kyriacos Felekkis, C. Petrou, and Chidi Evans Egwim. "Determination of viability in yeast cell culture for the study of some plant base insulin releasing and insulin like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum)." European Journal of Biology and Medical Science Research 12, no. 3 (2024): 66–92. https://doi.org/10.37745/ejbmsr.2013/vol12n36692.

Full text

Abstract:

The determination of viability in yeast cell culture for the study of some insulin – releasing and insulin – like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) was studied using standard techniques, and data analytical tools respectively. The yeast cell line was used for this study. Yeast cell measurement at OD600 was carried out with the assistance of a spectrophotometer. Yeast cell counting was done first with a hemocytometer to determine the yeast cell number (as a correlation between the OD600). The yeast cell was very viable 95.9%. The tryp

APA, Harvard, Vancouver, ISO, and other styles

8

Venkatesh, S., K. Muhamed, Mohan P. Mridhul, Asharaf V.V. Asheena, P. Anjitha, and A. Suresh. "Anti-Tumor and Anti-Oxidant Activity of Ethanolic Extract of Epipremnum Aureum Linn. Leaves against DAL Induced Tumor in Swiss Albino Mice." International Journal of Current Science Research and Review 04, no. 08 (2021): 1008–21. https://doi.org/10.47191/ijcsrr/V4-i8-18.

Full text

Abstract:

Abstract : Neoplasia literally means the process of “new growth,” and a new growth is called a neoplasm. The term tumor was originally applied to the swelling caused by inflammation. Neoplasms also may induce swellings, but by long precedent, the non-euplastic usage of tumor has passed into limbo; thus, the term is now equated with neoplasm. Oncology (Greek oncos = tumor) is the study of tumors or neoplasm’s. Cancer is the common term for all malignant tumors. The present study was designed to investigate the anti-tumor activity of ethanolic leaves extract of Epipremnum aureu

APA, Harvard, Vancouver, ISO, and other styles

9

Qin, Chen, and He Dongmei. "Mir-15a and Mir-16-1 Induce Apoptosis of Raji Cell Line." Blood 112, no. 11 (2008): 4492. http://dx.doi.org/10.1182/blood.v112.11.4492.4492.

Full text

Abstract:

Abstract MicroRNAs are endogenous small noncoding RNAs that regulate gene expression negatively at posttranscriptional level. Mir-15a and mir-16-1 are deleted or downregulated in the majority of chronic lymphomatic leukemia(CLL), which is the most common human leukemia and overexpresses the antiapoptotic B cell lymphoma2 (Bcl-2) protein. Here we investigated the effect of mir-15a and mir-16-1 on human lymhpomatic cell line Raji in induction of apoptosis. We transfected chemically synthesized mir-15a and mir-16-1 oligonucleotide into Raji cells using Lipofectamine 2000 reagent. At 24,48,72 hour

APA, Harvard, Vancouver, ISO, and other styles

10

Chang, Yung-Fu, and Harland W. Renshaw. "Pasteurella haemolytica leukotoxin: Comparison of 51chromium-release, trypan blue dye exclusion, and luminol-dependent chemiluminescence-inhibition assays for sensitivity in detecting leukotoxin activity." American Journal of Veterinary Research 47, no. 1 (1986): 134–38. https://doi.org/10.2460/ajvr.1986.47.01.134.

Full text

Abstract:

SUMMARY Dilutions of concentrated, dialyzed Pasteurella haemolytica culture supernatant were caused to react with bovine neutrophil (pmn) suspensions, and then the trypan blue dye exclusion (tbde), 51chromium (51Cr)-release, and luminol-dependent chemiluminescence-inhibition (ldcll) assays were done to compare their relative sensitivities in detecting biological activity of Ρ haemolytica leukotoxin (cytotoxin). The culture supernatant was concentrated approximately 200:1, and when caused to react as an undiluted preparation with bovine pmn, it was cytotoxic for 38.6% and 80.4% of pmn as determ

APA, Harvard, Vancouver, ISO, and other styles

More sources

Book chapters on the topic "Trypan blue dye exclusion assay"

1

Madar, InamulHasan, Ghazala Sultan, Ramachandran Chelliah, and Deog-Hwan Oh. "Screening for Anticancer Activity: Trypan Blue Exclusion Assay." In Methods in Actinobacteriology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1728-1_59.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Tripathi, Keshawanand, Yashdeep Srivastava, and Narendra Kumar. "Trypan blue dye exclusion assay: Principles, protocols, and applications in cell viability assessment." In Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis. Deep Science Publishing, 2025. https://doi.org/10.70593/978-93-49307-52-0_9.

Full text

Abstract:

Cell viability assessment is crucial for determining the number of live cells within a given sample. The Trypan blue dye exclusion test is a widely used method to distinguish between viable and nonviable cells based on their membrane integrity (Davis, 2011). Live cells, which maintain intact cell membranes, can exclude certain dyes like Trypan blue, whereas dead cells cannot. This technique involves mixing a cell suspension with Trypan blue and observing under a microscope: viable cells appear clear, while nonviable cells appear blue. This protocol is particularly valuable in cell culture stud

APA, Harvard, Vancouver, ISO, and other styles

3

Ude, Arinzechukwu, Kaiyven Afi-Leslie, Kelechi Okeke, and Emmanuel Ogbodo. "Trypan Blue Exclusion Assay, Neutral Red, Acridine Orange and Propidium Iodide." In Cytotoxicity [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105699.

Full text

Abstract:

Cytotoxicity and cell viability assessments are very important parameters that are widely used in fundamental research and drug development to determine the safety profile of toxic compounds. These assays measure the degree to which a substance can cause toxic damage to cells or cell death. There are different assays that have been employed to determine the cytotoxicity of substances. These assays either determine enzymatic function, cell viability, mitochondrial activity, lipid metabolism, cell proliferation and/or cell death. These assays entail use of different kinds of dyes such as trypan

APA, Harvard, Vancouver, ISO, and other styles

4

Shobana, G., N. Agnel Arul John, G. Hariharan, and G. Sridharan. "In Vitro Antioxidant and In Vitro Cytotoxic Potentials of Ancardium occidentale Linn. Against Hep G2 Cell Lines." In Harnessing Medicinal Plants in Cancer Prevention and Treatment. IGI Global, 2024. http://dx.doi.org/10.4018/979-8-3693-1646-7.ch013.

Full text

Abstract:

Cancer is a disease in which there is an uncontrolled multiplication and spread, within the body, of abnormal forms of the body's own cells. The Ethanol extract (EEAO) of Anacardium occidentale Linn was used for In-Vitro anticancer screening against HepG2 cell lines.Preliminary phytochemical analysis of the test drug was carried out and it revealed the presence of Tannin, Terpene, Quinone, Coumarin, Phenol. The results revealed that the extracts have significant Antioxidant potentials. In- vitro cytrotoxic assay such as tryphan blue dye exclusion, MTT and LDH releasing assays were carried out

APA, Harvard, Vancouver, ISO, and other styles

5

Tripathi, Keshawanand, Yashdeep Srivastava, and Narendra Kumar. "Evans blue dye exclusion assay: Mechanisms, protocols, and applications in cell viability analysis." In Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis. Deep Science Publishing, 2025. https://doi.org/10.70593/978-93-49307-52-0_10.

Full text

Abstract:

Evans blue is a dye that has the ability to penetrate the damaged cell membranes of deceased cells, resulting in a distinct blue staining. The Evans Blue assay is a widely used technique in biology and medicine to assess vascular permeability, particularly in studies involving inflammation, injury, or pathological conditions affecting blood vessel integrity. Named after the scientist Herbert M. Evans who first described its application in 1936, the assay utilizes Evans Blue dye, a synthetic azo dye with high affinity for serum albumin. In the Evans Blue assay, the dye is intravenously injected

APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Trypan blue dye exclusion assay"

1

Shatos, M., J. Doherty, D. Allen, and J. Hoak. "ALTERATIONS IN VASCULAR ENDOTHELIAL CELL FUNCTION BY OXYGEN-FREE RADICALS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643365.

Full text

Abstract:

The vascular endothelium is a target for oxidant-induced damage in many disease states including hyperoxia, inflammation, ischemia and reperfusion injury. However, little is known concerning oxidant injury to endothelial cells and its relationship to hemostasis. Our studies have focused on the ability of oxygen free radicals to injure and/or alter selected vascular endothelial cell functions pertinent to the regulation of hemostasis. Xanthine and xanthine oxidase, a well-characterized generating system for the production of the superoxide anion radical (O− 2) was used to sublethally injure hum

APA, Harvard, Vancouver, ISO, and other styles

2

M. ALI, Hanan, Asawer Salim TEMMA, and Omar Naji ALI. "ANALYTICAL STUDIES AND THE EFFECT OF TRYPAN BLUE IN HUMAN ESOPHAGUS CANCER CELLS." In VIII.International ScientificCongressofPure,AppliedandTechnological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress8-20.

Full text

Abstract:

Analytical studies of Trypan Blue (TB) diazo dye; showed, highest solubility in water without deviation in the linearity between a dipole moment constants and maximum wavelength. Because of the absorption spectrum of TB affected by using a range of different solvents via solvation and dielectric constant (D). The linear relationship between ʎmax and each function F(D), and (D-1)/(D+1), Shown that the D is the only operator that controls shift in the peak. Results also showed, that the deviation from the linear relationship of DMSO is explained on the basis of the formation of strong hydrogen b

APA, Harvard, Vancouver, ISO, and other styles

3

Ujihira, Masanobu, Yumi Matsumura, Chinatsu Kuroda, Koji Okaniwa, and Kiyoshi Mabuchi. "Effect of Cell Density on Post-Thaw Viability in Cryopreserved Artificial Tissue." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2220.

Full text

Abstract:

Abstract The effect of cell density on the post-thaw viability of cells in cryopreserved artificial tissue was studied. Human fibroblasts were three-dimensionally cultured for 2 days in a collagen sponge (ϕ20×1mm) as an extracellular matrix to imitate biological tissue (artificial tissue). Different cell densities for the artificial tissue were used, from 104 to 107 cells/cm3. Four artificial tissues were first stacked in a test chamber, then frozen at a slow or fast cooling rate (either 1 or 50°C/min) in a solution of Dulbecco’s Modified Eagle Medium, 20% Fetal Bovine Serum, and 10% dimethyls

APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Trypan blue dye exclusion assay"

1

ปทุมราช, สุทธิลักษณ์, ภาวพันธ์ ภัทรโกศล та สัญญา หกพุดซา. ผลของเหงือกปลาหมอดอกขาวต่อการเกิดหลอดเลือดใหม่ และต่อการเติบโตของ เซลล์มะเร็งปากมดลูก ที่ปลูกบนผิวหนังของหนูนู๊ดไมส์ : รายงานฉบับสมบูรณ์โครงการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2011. https://doi.org/10.58837/chula.res.2011.32.

Full text

Abstract:

วัตถุประสงค์ของการศึกษาครั้งนี้เพื่อศึกษาผลของสมุนไพรเหงือกปลาหมอดอกขาวที่สกัดด้วยน้ำต่อการเจริญเติบโตและการเกิดหลอดเลือดใหม่ของมะเร็งปากมดลูก โดยทำการศึกษาในหลอดทดลองเพื่อทดสอบผลการยับยั้งการเจริญของเซลล์มะเร็ง และทำการศึกษาในหนูนูดไมซ์ที่ได้รับการปลูกถ่ายเซลล์มะเร็งปากมดลูกใต้ชั้นผิวหนัง โดยเซลล์มะเร็งปากมดลูกที่ใช้เป็นชนิดที่บรรจุสารชีวะพันธุกรรมของฮิวแมนปาปิลโลมาไวรัส ชนิด 16 (Human papillomavirus type 16, (HPV)-16 DNA) และศึกษาผลของสารสกัดสมุนไพรเหงือกปลาหมอต่อชีวะโมเลกุลที่บ่งชี้การเกิดหลอดเลือดใหม่ของมะเร็ง และการตายของเซลล์มะเร็ง ได้แก่ VEGF และ P53 การศึกษาผลของสารสกัดสมุนไพรเหงือกปลา

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!