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Journal articles on the topic 'Trypan blue dye exclusion assay'

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Published: 4 June 2025

Last updated: 1 August 2025

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1

Bussadori, Sandra Kalil, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, et al. "Cytotoxicity assessment of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste." ConScientiae Saúde 9, no. 3 (2010): 354–59. http://dx.doi.org/10.5585/conssaude.v9i3.2224.

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Introduction: Casein phosphopeptides (CPP) have been shown to be good carriers of calcium, phosphate, and hydroxide ions to promote enamel remineralization with applications in oral care products, professional dental products, and food products. Objectives: Evaluate the cytotoxicity of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste in rat fibroblasts. Materials and methods: Cytotoxicity was measured by the Trypan blue dye exclusion assay and the MTT assay. Results: Long term (1, 3, 5, and 7 days) and short term tests (0, 4, 8, and 12 hours) were performed with the Trypan b

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Raajshree.r, Khoushika, and Brindha Durairaj. "IN VITRO ANTICANCER POTENTIAL OF BIOSYNTHESIZED ZINC OXIDE NANOPARTICLES FROM THE SEAWEED TURBINARIA CONOIDES." Asian Journal of Pharmaceutical and Clinical Research 11, no. 5 (2018): 127. http://dx.doi.org/10.22159/ajpcr.2018.v11i5.22224.

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Objective: The objective of this study is to investigate the anticancer potential of zinc oxide nanoparticles (ZnO-NPs) and hydroethanolic extract of Turbinaria conoides (HETC) against Dalton’s lymphoma ascites (DLA) cell line. Methods: Nanoparticles were synthesized from the HETC. An ultraviolet-visible spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology, and elemental composition of ZnO-NPs were also analyzed using scanning electron microscope-energy dispersive X-ray diffraction. The cytotoxic activity of ZnO-NPs and HETC was evaluated by 3-(4,5-di

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Vega, Maria V., Samuel K. Maheswaran, Joel R. Leininger, and Trevor R. Ames. "Adaptation of a colorimetric microtitration assay for quantifying Pasteurella haemolytica A1 leukotoxin and antileukotoxin." American Journal of Veterinary Research 48, no. 11 (1987): 1559–64. https://doi.org/10.2460/ajvr.1987.48.11.1559.

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SUMMARY A colorimetric microtitration assay was adapted to quantify the cytotoxicity of Pasteurella haemolytica A1 leukotoxin to bovine neutrophils used as target cells. The viability of leukotoxin-treated target cells was detected by use of a tetrazolium dye that living cells reduced to dark blue formazan. The amount of formazan formed (which was quantified by use of an elisa plate reader) was directly proportional to the number of viable target cells. This assay system also was used to measure leu-kotoxin-neutralization antibody titers of bovine serum and lung lavage specimens obtained durin

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Et al., Hussein. "Trypan Blue Exclusion Assay Verifies in Vitro Cytotoxicity of New Cis-Platinum (II) Complex in Human Cells." Baghdad Science Journal 16, no. 3 (2019): 0555. http://dx.doi.org/10.21123/bsj.2019.16.3.0555.

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Various assays are used to determine the toxic effects of drugs at cellular levels in vitro. One of these methods is the dye exclusion assay, which measures membrane integrity in the presence of Trypan blue. Trypan blue the dye which was used in this study to investigate cytotoxic effect of a new Cis –dichloroplatinum (II) complex [(Qu)2PtCl2] on the viability of polymorphonuclear cells (PMNs). Three concentrations of platinum complex were prepared (70, 35and 17.5 µg/ ml) and the results revealed that the percentage of cell viability decreased as the platinum complex concentration increased in

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Monte Neto, Rubens L., M. A. Sousa, Celidarque S. Dias, José M. Barbosa Filho, and Márcia R. Oliveira. "Yangambin Cytotoxicity: A Pharmacologically Active Lignan Obtained from Ocotea duckei Vattimo (Lauraceae)." Zeitschrift für Naturforschung C 63, no. 9-10 (2008): 681–86. http://dx.doi.org/10.1515/znc-2008-9-1012.

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The in vitro cytotoxic potential of yangambin was evaluated. Yangambin is a pharmacologically active furofuran lignan obtained from the leaves of Ocotea duckei. It is the major compound from the lignoids fraction. Yangambin presented low cytotoxicity in all in vitro models analyzed. Its cytotoxicity to murine macrophages was measured by the Trypan blue dye exclusion test and MTT reduction assay, resulting in high CC50 values of 187.0 μg/mL (383.3 μm) and 246.7 μg/mL (504.3 μm), respectively. The difference obtained in the inhibitory concentrations aforementioned can be explained, at least in p

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Ahmad, Shama, Aftab Ahmad, B. Kelly Schneider, and Carl W. White. "Cholesterol Interferes with the MTT Assay in Human Epithelial-Like (A549) and Endothelial (HLMVE and HCAE) Cells." International Journal of Toxicology 25, no. 1 (2006): 17–23. http://dx.doi.org/10.1080/10915810500488361.

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Metabolically active cells are able to convert the MTT [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide] dye to blue formazan. This is the basis of the MTT assay, which is among the most widely used screening methods to evaluate cell viability and proliferation. When testing the effects of cholesterol products on the viability of human pulmonary epithelial-like A549 cells using trypan blue staining (cell numbers) and the MTT assay, results were inconsistent. The MTT assay indicated greater than 50% loss of viability with exposure of cells to cholesterol, whereas there was no decrea

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Aloysius, Morris Terfa, Eleni Andreou, Kyriacos Felekkis, C. Petrou, and Chidi Evans Egwim. "Determination of viability in yeast cell culture for the study of some plant base insulin releasing and insulin like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum)." European Journal of Biology and Medical Science Research 12, no. 3 (2024): 66–92. https://doi.org/10.37745/ejbmsr.2013/vol12n36692.

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The determination of viability in yeast cell culture for the study of some insulin – releasing and insulin – like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) was studied using standard techniques, and data analytical tools respectively. The yeast cell line was used for this study. Yeast cell measurement at OD600 was carried out with the assistance of a spectrophotometer. Yeast cell counting was done first with a hemocytometer to determine the yeast cell number (as a correlation between the OD600). The yeast cell was very viable 95.9%. The tryp

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Venkatesh, S., K. Muhamed, Mohan P. Mridhul, Asharaf V.V. Asheena, P. Anjitha, and A. Suresh. "Anti-Tumor and Anti-Oxidant Activity of Ethanolic Extract of Epipremnum Aureum Linn. Leaves against DAL Induced Tumor in Swiss Albino Mice." International Journal of Current Science Research and Review 04, no. 08 (2021): 1008–21. https://doi.org/10.47191/ijcsrr/V4-i8-18.

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Abstract : Neoplasia literally means the process of “new growth,” and a new growth is called a neoplasm. The term tumor was originally applied to the swelling caused by inflammation. Neoplasms also may induce swellings, but by long precedent, the non-euplastic usage of tumor has passed into limbo; thus, the term is now equated with neoplasm. Oncology (Greek oncos = tumor) is the study of tumors or neoplasm’s. Cancer is the common term for all malignant tumors. The present study was designed to investigate the anti-tumor activity of ethanolic leaves extract of Epipremnum aureu

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Qin, Chen, and He Dongmei. "Mir-15a and Mir-16-1 Induce Apoptosis of Raji Cell Line." Blood 112, no. 11 (2008): 4492. http://dx.doi.org/10.1182/blood.v112.11.4492.4492.

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Abstract MicroRNAs are endogenous small noncoding RNAs that regulate gene expression negatively at posttranscriptional level. Mir-15a and mir-16-1 are deleted or downregulated in the majority of chronic lymphomatic leukemia(CLL), which is the most common human leukemia and overexpresses the antiapoptotic B cell lymphoma2 (Bcl-2) protein. Here we investigated the effect of mir-15a and mir-16-1 on human lymhpomatic cell line Raji in induction of apoptosis. We transfected chemically synthesized mir-15a and mir-16-1 oligonucleotide into Raji cells using Lipofectamine 2000 reagent. At 24,48,72 hour

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Chang, Yung-Fu, and Harland W. Renshaw. "Pasteurella haemolytica leukotoxin: Comparison of 51chromium-release, trypan blue dye exclusion, and luminol-dependent chemiluminescence-inhibition assays for sensitivity in detecting leukotoxin activity." American Journal of Veterinary Research 47, no. 1 (1986): 134–38. https://doi.org/10.2460/ajvr.1986.47.01.134.

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SUMMARY Dilutions of concentrated, dialyzed Pasteurella haemolytica culture supernatant were caused to react with bovine neutrophil (pmn) suspensions, and then the trypan blue dye exclusion (tbde), 51chromium (51Cr)-release, and luminol-dependent chemiluminescence-inhibition (ldcll) assays were done to compare their relative sensitivities in detecting biological activity of Ρ haemolytica leukotoxin (cytotoxin). The culture supernatant was concentrated approximately 200:1, and when caused to react as an undiluted preparation with bovine pmn, it was cytotoxic for 38.6% and 80.4% of pmn as determ

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Altman, Steven A., Lisa Randers, and Govind Rao. "Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations." Biotechnology Progress 9, no. 6 (1993): 671–74. http://dx.doi.org/10.1021/bp00024a017.

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Sarker, K., A. Ghosh, S. Mishra, A. Saha, and S. Sen. "Design, Synthesis, Molecular Docking and in vitro Evaluation of N-(4-Methoxyphenylsulfonyl)pyrrolidine-2-carboxylic Acid Analogues as Antiangiogenic and Anticancer Agents on Multiple Myeloma." Asian Journal of Chemistry 32, no. 12 (2020): 3079–86. http://dx.doi.org/10.14233/ajchem.2020.22957.

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A series of N-(4-methoxyphenylsulfonyl)pyrrolidine-2-carboxylic acid analogs were designed as bioisosteres of a major metabolite of thalidomide, i.e., N-(o-carboxybenzoyl)-D,L-glutamic acid. Compounds 2b, 2d, 2f, 2i and 2k exhibited anticancer activity on multiple myeloma (RPMI 8226) by MTS assay and were tested for primary antiangiogenic activity on HUVEC cell line by MTT assay. Compound 2f was excluded from further study as it was found to be cytotoxic to normal epithelial cells. 2b, 2d, 2i and 2k were found to have primary antiangiogenic activity along with low cytotoxicity on normal vero c

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Carey, Doug, Jun Wu, Hiroshi Sugiyama, and Tai-Wing Wu. "Validation of the morphologic end point of necrosis in rat hepatocytes subjected to oxyradical damage." Biochemistry and Cell Biology 69, no. 10-11 (1991): 689–94. http://dx.doi.org/10.1139/o91-103.

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We have used phase-contrast microscopy to determine a necrotic end point of the order of minutes in primary hepatocytes exposed to oxyradicals generated with xanthine oxidase plus hypoxanthine. This study examines whether the morphologic end point thus determined agrees with other criteria of cell necrosis. When 95–100% of the cells were shown to be necrotic by our morphologic assay, transmission electron microscopy confirmed definitive subcellular evidence of cell death, trypan blue exclusion revealed a 92% loss in the ability of cells to exclude the dye, and there was a 47% specific release

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GHOSH, A., A. SAHA, K. SARKER, S. MISHRA, and S. SEN. "Design, Synthesis, Molecular Docking and in vitro Evaluation of N-(4-Ethoxyphenylsulfonyl)pyrrolidine-2-carboxylic Acid Analogues as Antiangiogenic and Anticancer Agents on Multiple Myeloma." Asian Journal of Chemistry 33, no. 4 (2021): 727–33. http://dx.doi.org/10.14233/ajchem.2021.23064.

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In present work, N-(4-ethoxyphenylsulfonyl)pyrrolidine-2-carboxylic acid analogs were designed, synthesized and biologically evaluated as an antiangiogenic and anticancer agent on multiple myeloma. Compounds 3i, 3k and 3m exhibited the cytotoxic action on human multiple myeloma cell line RPMI8226 with IC50 (μM) value 3.72, 3.89 2.28, respectively. These compounds possessed the antiangiogenic property and are selectively cytotoxic to cancer cells, as observed from the in vitro study of human umbilical vein endothelial cell (HUVEC) and African green monkey epithelial cell (VERO), respectively. A

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Kuzmits, Rudolf, Paul Aiginger, Matthias M. Müller, Günter Steurer, and Werner Linkesch. "Assessment of the sensitivity of leukaemic cells to cytotoxic drugs by bioluminescence measurement of ATP in cultured cells." Clinical Science 71, no. 1 (1986): 81–88. http://dx.doi.org/10.1042/cs0710081.

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1. A short-term test in vitro is described, which can be used to detect resistance to cytostatic agents in leukaemic cells. Leukaemic cell suspensions were incubated with cytostatic agents and the resulting intracellular ATP concentrations were measured by a bioluminescence ATP assay. 2. There was a clear dose-effect relationship in acute leukaemia and chronic lymphocytic leukaemia cells for drugs used in the treatment of leukaemias. A good correlation was found between the ATP content of leukaemic cells and cell viability as determined by the trypan blue dye exclusion test. 3. Preliminary ind

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Liu, Tielong, Yonggang Liu, Jiashi Cao та ін. "ILKAP Binding to and Dephosphorylating HIF-1α is Essential for Apoptosis Induced by Severe Hypoxia". Cellular Physiology and Biochemistry 46, № 6 (2018): 2500–2507. http://dx.doi.org/10.1159/000489656.

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Background/Aims: Integrin-linked kinase-associated phosphatase (ILKAP), a serine/threonine phosphatase that belongs to the protein phosphatase 2C family, has a role in cell survival and apoptosis. Hypoxia-inducible factor 1α (HIF-1α) is the key transcription factor in the response to oxygen deficiency in mammals. Direct phosphorylation and dephosphorylation of HIF-1α affect its function. The present study investigated the role of ILKAP on HIF-1α dephosphorylation and cell behavior. Methods: HIF-1α was induced by hypoxia. Physical binding between ILKAP and HIF-1α was demonstrated by a co-immuno

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Gonçalves, Paula P., Manoj K. Singh, Virgília S. Silva, et al. "Automated high-throughput screening of carbon nanotube-based bio-nanocomposites for bone cement applications." Pure and Applied Chemistry 83, no. 11 (2011): 2063–69. http://dx.doi.org/10.1351/pac-con-11-04-06.

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In this work we demonstrate the potential of using an automated cell viability analyzer for developing high-throughput screening of orthopedic bioactive materials. We used a biomaterial of carbon nanotubes (CNTs)-based composite integrated with hydroxyapatite/polymethyl methacrylate (HA/PMMA) with controlled physical and chemical properties to evaluate the usefulness of morphometric analysis in conjunction with trypan blue dye exclusion assays in MG63 cell cultures. The MG63 cell line, derived from human bone osteo-sarcoma, is often used as a model for studying osteoblast-like cellular respons

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Crescenzi, Elvira, Linda Varriale, Mariangela Iovino, Angela Chiaviello, Bianca Maria Veneziani, and Giuseppe Palumbo. "Photodynamic therapy with indocyanine green complements and enhances low-dose cisplatin cytotoxicity in MCF-7 breast cancer cells." Molecular Cancer Therapeutics 3, no. 5 (2004): 537–44. http://dx.doi.org/10.1158/1535-7163.537.3.5.

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Abstract Objective: We investigated the effects of photodynamic therapy (PDT) combined with low-dose chemotherapy on breast cancer cells. Photodynamic treatment was administered by irradiating indocyanine green-preloaded MCF-7 cells with an IR diode laser source at 805 nm; cisplatin was used for chemotherapy. Methods: The dose-response phenomena associated with the two treatments administered individually and together were evaluated with the following tests: trypan blue dye exclusion, 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay, clonogenic survival, thymidine and methio

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Cariddi, L. N., M. C. Sabini, F. M. Escobar, et al. "In VitroandIn VivoCytogenotoxic Effects of Hot Aqueous Extract ofAchyrocline satureioides(Lam.) DC." BioMed Research International 2015 (2015): 1–12. http://dx.doi.org/10.1155/2015/270973.

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In this work we extend the toxicological studies of hot aqueous extract ofA. satureioides(As-HAE) evaluating cytotoxic and apoptotic effects on human peripheral blood mononuclear cells (PBMCs). We also determine genotoxic action of this extractin vivo. In addition, the extract was chemically characterized. Finally, we established a comparison with previous data of cold aqueous extract. The As-HAE induced cytotoxicity on PBMCs determined by trypan blue dye exclusion (CC50= 653 μg/mL) and MTT (CC50= 588 μg/mL) assays being more toxic than cold extract. However, As-HAE as well as cold extract did

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Yamashita, Kyohei, Ryoma Tagawa, Yoshikazu Higami, and Eiji Tokunaga. "Noninvasive and Safe Cell Viability Assay for Breast Cancer MCF-7 Cells Using Natural Food Pigment." Biology 9, no. 8 (2020): 227. http://dx.doi.org/10.3390/biology9080227.

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A dye exclusion test (DET) was performed to determine the viability of human breast cancer cells MCF-7, using natural food pigments as compared with trypan blue (TB), a typical synthetic dye for DET known to exhibit teratogenicity and cytotoxicity. We demonstrated that Monascus pigment (MP) is noninvasive to living cells and can effectively stain only dead cells. This study is the first verification of the applicability of MP to cancer cells. The appropriate MP concentration was 0.4% (0.02% as the concentration of pure MP) and all the dead cells were stained within 10 min. We found that the ce

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Saha, Abhijit, Koushik Sarker, Avijit Ghosh, Suvasish Mishra, and Subrata Sen. "RATIONAL DESIGN, SYNTHESIS AND IN VITRO EVALUATION OF N-(4-ETHOXYPHENYLSULFONYL)-L-GLUTAMIC ACID ANALOGUES AS ANTIANGIOGENIC AND ANTICANCER AGENTS ON MULTIPLE MYELOMA." INDIAN DRUGS 57, no. 12 (2021): 26–35. http://dx.doi.org/10.53879/id.57.12.12807.

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We report the rational design, synthesis and evaluation of the anticancer and antiangiogenic activity of the N-(4-ethoxyphenylsulfonyl)-L-glutamic acid analogs on multiple myeloma. From the series, compound 2c, 2f, and 2h exhibit cytotoxic action on human multiple myeloma cell line RPMI8226 with IC50 (μM) value 2.72, 2.24, and 1.81, respectively. These compounds possess the antiangiogenic property and are selectively cytotoxic to cancer cells, as observed from the in vitro study of Human Umbilical Vein Endothelial Cell (HUVEC) and African green monkey kidney epithelial cell (VERO), respectivel

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Ghosh, Dipanwita, Preeti Mangar, Abhinandan Choudhury, et al. "Characterization of a hemolytic and antibiotic-resistant Pseudomonas aeruginosa strain S3 pathogenic to fish isolated from Mahananda River in India." PLOS ONE 19, no. 3 (2024): e0300134. http://dx.doi.org/10.1371/journal.pone.0300134.

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Virulent strain Pseudomonas aeruginosa isolated from Mahananda River exhibited the highest hemolytic activity and virulence factors and was pathogenic to fish as clinical signs of hemorrhagic spots, loss of scales, and fin erosions were found. S3 was cytotoxic to the human liver cell line (WRL-68) in the trypan blue dye exclusion assay. Genotype characterization using whole genome analysis showed that S3 was similar to P. aeruginosa PAO1. The draft genome sequence had an estimated length of 62,69,783 bp, a GC content of 66.3%, and contained 5916 coding sequences. Eight genes across the genome

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Zorić, Nataša, Nevenka Kopjar, Klara Kraljić, Nada Oršolić, Siniša Tomić, and Ivan Kosalec. "Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study." Acta Pharmaceutica 66, no. 3 (2016): 411–21. http://dx.doi.org/10.1515/acph-2016-0033.

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Abstract Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL-1 and C. dubliniensis with MIC

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Das, PP, AP Shaik, and K. Jamil. "Genotoxicity induced by pesticide mixtures: in-vitro studies on human peripheral blood lymphocytes." Toxicology and Industrial Health 23, no. 8 (2007): 449–58. http://dx.doi.org/10.1177/0748233708089040.

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To assess the damage caused by pesticides and their mixtures on humans, we designed in-vitro experiments to evaluate their cytotoxicity and genotoxicity. Three equimolar pesticide mixtures were investigated for their capability to affect cultured human peripheral blood lymphocytes. The LC50 values for cytotoxicity, using standard trypan blue dye exclusion and calculated by probit analysis, were 4.18, 5.76, and 7.5 μM for endosulfan, carbofuran, and monocrotophos, respectively. When combined in equimolar concentrations, the LC50 values for cytotoxicity were 0.7, 0.9, and 1.0 μM for monocrotopho

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Bass, Noa, Mruga Parekh, Prabodh Satyal, et al. "Manuka Essential Oil Triggers Apoptosis and Activation of c-Jun N-Terminal Kinase in Fibroblasts and Fibrosarcoma Cells." Molecules 29, no. 21 (2024): 5168. http://dx.doi.org/10.3390/molecules29215168.

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Manuka essential oil has long been used in traditional medicine, though the effects of the oil on cancer cells have limited studies. The goal of this project was to treat cancer cell lines with manuka essential oil at different concentrations and to ascertain the effects on the cell proliferation of normal fibroblast (CUA-4) and on fibrosarcoma (HT-1080) cells. Cell lines were grown on 24-well plates, and subconfluent cultures were treated with varying concentrations of manuka oil for 24 h. The effect of the oil on proliferation and viability was measured through direct cell counting using try

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Mukherjee, Soumalya, Mitali Ray, and Sajal Ray. "A report of hailstorm damage to two species of freshwater sponge (Demospongiae: Haplosclerida: Spongillidae) populations of West Bengal, India." Journal of Threatened Taxa 8, no. 4 (2016): 8719. http://dx.doi.org/10.11609/jott.1739.8.4.8719-8727.

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Selected freshwater bodies in Jagaddal of North 24 Parganas in West Bengal, India are inhabited by two species of sponge, Eunapius carteri (Bowerbank, 1863) and Spongilla alba (Carter, 1849) (Porifera: Demospongiae: Spongillidae). Most of these wetlands are perennial ponds without a history of aquaculture and toxin contamination. On 22 March 2014, the entire area of Jagaddal experienced an unprecedented hailstorm associated with a sharp decline of environmental temperature from 35 0C to 21 0C within 10–15 minutes. The hailstorm associated with torrential rain lasted for about 30 minutes. The n

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Biernacka, K. M., C. C. Uzoh, L. Zeng, et al. "Hyperglycaemia-induced chemoresistance of prostate cancer cells due to IGFBP2." Endocrine-Related Cancer 20, no. 5 (2013): 741–51. http://dx.doi.org/10.1530/erc-13-0077.

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Clinically relevant prostate cancer (PCa) is more frequent in Westernised societies and increasingly men have co-morbidities associated with a Western lifestyle, primarily diabetes, characterised by hyperinsulinaemia and hyperglycaemia. IGFs and their binding proteins (IGFBPs) are important mediators of the effects of nutrition on growth and play a key role in the development of PCa. We used DU145, PC3 and LNCaP PCa cell lines to examine how hyperglycaemia altered their response to docetaxel. Trypan Blue dye-exclusion assay was used to determine the percentage of cell death. Protein abundance

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Moon, Seo Hyun, and Min Young Kim. "Effect of dose-rate on nitric oxide-induced apoptosis in A375 human melanoma cells." Tropical Journal of Pharmaceutical Research 23, no. 4 (2024): 675–81. http://dx.doi.org/10.4314/tjpr.v23i4.1.

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Purpose: To investigate the effect of dose-rate on nitric oxide (NO)-induced apoptosis in A375 human melanoma cells. Methods: The A375 cells were exposed to NO at a steady-state concentration of 7 μM, similar to the level estimated to occur in vivo in inflamed tissues, and delivered by diffusion through silastic tubing. Trypan blue dye exclusion assay was used to assess cell proliferation and viability. Cell cycle was studied by propidium iodide (PI) staining while Annexin V-FITC and PI assays were used to evaluate apoptosis. Western blotting assay was carried out to determine protein levels o

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SILVEIRA, Keli Cristina Simões da, Cassiana Macagnan VIAU, Josiane Raskopf COLARES, Jenifer SAFFI, Norma Possa MARRONI, and Marilene PORAWSKI. "CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS." Arquivos de Gastroenterologia 52, no. 1 (2015): 65–71. http://dx.doi.org/10.1590/s0004-28032015000100014.

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Background Renal failure is a frequent and serious complication in patients with decompensated cirrhosis. Objectives We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis. Methods Secondary biliary cirrhosis was induced in rats by ligation of the common bile duct. We measured TBARS, ROS and mitochondrial membrane potential in kidney as markers of oxidative stress, and activities of the antioxidant enzymes. Relative cell viability was determined by trypan blue dye-exclusion assay. Annexin V-PE was used with a vital dye, 7-AAD, to di

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Azevedo, Cledson Lima de, Márcia Martins Marques, and Antonio Carlos Bombana. "Cytotoxic effects of cyanoacrylates used as retrograde filling materials: an in vitro analysis." Pesquisa Odontológica Brasileira 17, no. 2 (2003): 113–18. http://dx.doi.org/10.1590/s1517-74912003000200003.

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Cyanoacrylate has been used in medicine and dentistry for many years. It has been used as a postextraction dressing and retrograde filling material in endodontic surgery. The aim of this study was to evaluate the cytotoxic effects of Histoacryl and other two homologue ethyl cyanoacrylates, Super Bonder and Ultrabond, on cultured fibroblasts, using the Trypan blue dye exclusion assay. The cyanoacrylates were applied to round glass coverslips, which were placed in contact with NIH 3T3 cells. After 0, 6, 12 and 24 h (short-term assay; viability) and 1, 3, 5 and 7 days (long-term assay; survival),

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Gokulakrishnaa, R. K., and Selvamuthukumaran Thirunavukkarasu. "Bioassay Techniques in Entomological Research." International Journal of Plant & Soil Science 35, no. 16 (2023): 363–73. http://dx.doi.org/10.9734/ijpss/2023/v35i163163.

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BIOASSAY (Bio - life, Assay - determination) is the measurement of the potency of any stimulus (physical, chemical, biological, and psychological) and the reactions that it produces in living matter. In Entomology, to know about the potency, Specificity of any compounds, standardization of doses, and identify modes of action bioassay is needed. In addition to the basic bioassay methods such as poison food, dry film techniques, etc., depending upon the researcher’s necessity, they are modified or newly developed. Diet covering and diet incorporation for lepidopterans and homopterans have been d

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Lee, Hyun Joo, Ju Yeon Ban, Sang Bum Koh, et al. "Polygalae Radix Extract Protects Cultured Rat Granule Cells Against Damage Induced by NMDA." American Journal of Chinese Medicine 32, no. 04 (2004): 599–610. http://dx.doi.org/10.1142/s0192415x04002235.

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Polygalae Radix (PR) from Polygala tenuifolia (Polygalaceae) is traditionally used in China and Korea, as this herb has a sedative, anti-inflammatory and antibacterial agent. To extend our understanding of the pharmacological actions of PR in the CNS on the basis of its CNS inhibitory effect, the present study examined whether PR has the neuroprotective action against N-methyl-D-aspartate (NMDA)-induced cell death in primarily cultured rat cerebellar granule neurons. PR, over a concentration range of 0.05 to 5 μg/ml, inhibited NMDA (1 mM)-induced neuronal cell death, which was measured by a tr

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Badmus, J. A., O. E. Ekpo, A. A. Hussein, M. Meyer, and D. C. Hiss. "Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract ofHolarrhena floribunda(G. Don)." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–11. http://dx.doi.org/10.1155/2015/756482.

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Natural plant products with potent growth inhibition and apoptosis induction properties are extensively being investigated for their cancer chemopreventive potential.Holarrhena floribunda(HF) is used in a wide range of traditional medicine practices. The present study investigated the antiproliferative and apoptosis induction potential of methanolic leaf extracts of HF against breast (MCF-7), colorectal (HT-29), and cervical (HeLa) cancer cells relative to normal KMST-6 fibroblasts. The MTT assay in conjunction with the trypan blue dye exclusion and clonogenic assays were used to determine the

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Mendoza-Fuentes, Alan, Elena González-Burgos, Omar Emiliano Aparicio Trejo, et al. "The cytotoxicity effect of 7-hydroxy-3,4-dihydrocadalene from Heterotheca inuloides and semisynthetic cadalenes derivates towards breast cancer cells: involvement of oxidative stress-mediated apoptosis." PeerJ 11 (June 20, 2023): e15586. http://dx.doi.org/10.7717/peerj.15586.

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Background Heterotheca inuloides, traditionally employed in Mexico, has demonstrated anticancer activities. Although it has been proven that the cytotoxic effect is attributed to cadinane-type sesquiterpenes such as 7-hydroxy-3,4-dihydrocadalene, the mechanism of action by which these agents act in tumor lines and their regulation remain unknown. This study was undertaken to investigate for first time the cytotoxic activity and mechanism of action of 7-hydroxy-3,4-dihydrocadalene and two semi-synthetic cadinanes derivatives towards breast cancer cells. Methods Cell viability and proliferation

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Salihu, Rabiu, Saiful Izwan Abd Razak, Mohd Helmi Sani, Mohammed Ahmad Wsoo, Nurliyana Ahmad Zawawi, and Shafinaz Shahir. "Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration." PLOS ONE 19, no. 12 (2024): e0312396. https://doi.org/10.1371/journal.pone.0312396.

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Bacterial cellulose (BC) is a novel biocompatible polymeric biomaterial with a wide range of biomedical uses, like tissue engineering (TE) scaffolds, wound dressings, and drug delivery. Although BC lacks good cell adhesion due to limited functionality, its tunable surface chemistry still holds promise. Here, hydroxyapatite (HA) was incorporated into a citrate-modified BC (MBC) using the biomimetic synthesis in simulated body fluid (SBF). Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), thermal gravimetric analysis (T

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Bonfanti-Azzolin, Gabriela, Andressa Leal Zambra, Bruna Morgan da Silva, et al. "Toxicity assessment of Syzygium jambos and Solanum guaraniticum hydroethanolic leaf extracts thru Artemia salina lethality and spleen lymphocyte cytotoxicity tests." International Journal for Innovation Education and Research 7, no. 10 (2019): 133–43. http://dx.doi.org/10.31686/ijier.vol7.iss10.1758.

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The aim of this study was to investigate the toxicity of Syzygium jambos and Solanum guaraniticum leaf extracts, through Artemia salina lethality testing and cytotoxicity evaluations in rat spleen lymphocytes, using methyl tetrazolium (MTT), neutral red uptake (NRU), trypan blue dye exclusion, and lactate dehydrogenase (LDH) leakage assays, besides by their in vitro effects on acetylcholinesterase (AChE) activity. The LC50 calculated in the A. salina bioassay demonstrated that both extracts might well be toxic. Solanum guaraniticum presented cytotoxic effects against lymphocytes, as demonstrat

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Almuqbil, Rashed M. "Brucine Entrapped Titanium Oxide Nanoparticle for Anticancer Treatment: An In Vitro Study." Advances in Pharmacological and Pharmaceutical Sciences 2024 (March 18, 2024): 1–10. http://dx.doi.org/10.1155/2024/4646855.

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Backgroundand Objective. The public’s health has been seriously threatened by cervical cancer during recent times. In terms of newly diagnosed cases worldwide, it ranks as the ninth most prevalent malignancy. Multiple investigations have proven that nanoparticles can effectively combat cancer due to their small dimensions and extensive surface area. In the meantime, bioactive compounds which are biocompatible are being loaded onto nanoparticles to promote cancer therapy. The current study investigates the anticancerous potential of Brucine-entrapped titanium oxide nanoparticles (TiO2 NPs) in c

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Yamashita, Kyohei, Koji Yamada, Kengo Suzuki, and Eiji Tokunaga. "Noninvasive and safe cell viability assay forEuglena gracilisusing natural food pigment." PeerJ 7 (April 4, 2019): e6636. http://dx.doi.org/10.7717/peerj.6636.

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Noninvasive and safe cell viability assay is required in many fields such as regenerative medicine, genetic engineering, single-cell analysis, and microbial food culture. In this case, a safe and inexpensive method which is a small load on cells and the environment is preferable without requiring expensive and space-consuming equipment and a technician to operate. We examined eight typical natural food pigments to findMonascuspigment (MP) or anthocyanin pigment (AP) works as a good viability indicator of dye exclusion test (DET) forEuglena graciliswhich is an edible photosynthetic green microa

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Prasad, Paindla, and Mamidala Estari. "Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Acalypha indica (L.) Plant Leaves Extract." American Journal of Science and Medical Research 8, no. 3 (2022): 1–4. https://doi.org/10.5281/zenodo.7213389.

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<strong>ABSTRACT</strong> HIV (Human Immunodeficiency Virus) the virus that bases AIDS, is one of the newest zones of medical research nowadays. The objective of the study is to test the HIV-1 reverse transcriptase inhibitory efficiency of <em>Acalypha indica</em> leaves extracts. The leaves of <em>Acalypha indica</em>, were collected from the selected places of Adilabad District. Leaves were exposed to size reduction to get coarse powder and extracted by sequential maceration method using non-polar to polar solvents. Cell viability was determined in contradiction of PBMC cells by the trypan-b

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Sobolewska, Ewa, Piotr Makowiecki, Justyna Drozdowska, et al. "Cytotoxic Potential of Denture Adhesives on Human Fibroblasts—In Vitro Study." Materials 15, no. 4 (2022): 1583. http://dx.doi.org/10.3390/ma15041583.

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(1) In recent years, there has been a significant increase in the availability of denture adhesives for stabilizing removable dentures. The aim of the present study was to assess the cytotoxicity of three denture adhesives on human fibroblasts. (2) Methods: Three denture adhesives were analyzed. Fibroblast cultures were established for the study and control groups in order to assess the incidence of necrosis and to evaluate the microscopic intracellular alterations induced. Following incubation with (study groups) or without adhesives (control group), trypan blue dye exclusion assay was used t

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Sun, Shangfan, Yi Zhang, Weili Xu, Bin Wang, and Jing Chen. "Mitochondrial dysfunction is involved in the cellular activity inhibition by eleutheroside B in SMMC-7721 and HeLa cells." Human & Experimental Toxicology 41 (January 2022): 096032712210890. http://dx.doi.org/10.1177/09603271221089006.

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Eleutheroside B, also known as syringin, has been shown to have various pharmacological activities such as anti-inflammatory, anti-irradiation and antidepressant, but there are few studies on its anti-cancer activity. Its anti-tumor effect on SMMC-7721 cells has not been revealed. Moreover, whether it induces autophagy is still unclear. Thus, the present study investigated whether Eleutheroside B induces apoptosis, autophagy and cellular motility in SMMC-7721 cells and HeLa cells, and explored the underlying molecular mechanisms. SMMC-7721 cells and HeLa cells treated with Eleutheroside B and

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Hussain, Fahad, Fahad Imtiaz Rahman, Poushali Saha, et al. "Synthesis of Sugar and Nucleoside Analogs and Evaluation of Their Anticancer and Analgesic Potentials." Molecules 27, no. 11 (2022): 3499. http://dx.doi.org/10.3390/molecules27113499.

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Chemical modification of sugars and nucleosides has a long history of producing compounds with improved selectivity and efficacy. In this study, several modified sugars (2–3) and ribonucleoside analogs (4–8) have been synthesized from α-d-glucose in a total of 21 steps. The compounds were tested for peripheral anti-nociceptive characteristics in the acetic acid-induced writhing assay in mice, where compounds 2, 7, and 8 showed a significant reduction in the number of writhes by 56%, 62%, and 63%, respectively. The compounds were also tested for their cytotoxic potential against human HeLa cell

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Tseng, Tsui-Hwa, Chau-Jong Wang, Yean-Jang Lee, et al. "Suppression of the Proliferation of Huh7 Hepatoma Cells Involving the Downregulation of Mutant p53 Protein and Inactivation of the STAT 3 Pathway with Ailanthoidol." International Journal of Molecular Sciences 23, no. 9 (2022): 5102. http://dx.doi.org/10.3390/ijms23095102.

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Ailanthoidol (ATD) has been isolated from the barks of Zanthoxylum ailanthoides and displays anti-inflammatory, antioxidant, antiadipogenic, and antitumor promotion activities. Recently, we found that ATD suppressed TGF-β1-induced migration and invasion of HepG2 cells. In this report, we found that ATD exhibited more potent cytotoxicity in Huh7 hepatoma cells (mutant p53: Y220C) than in HepG2 cells (wild-type p53). A trypan blue dye exclusion assay and colony assay showed ATD inhibited the growth of Huh7 cells. ATD also induced G1 arrest and reduced the expression of cyclin D1 and CDK2. Flow c

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Hussein Al Ali, Samer Hasan, Mothanna Al-Qubaisi, Mohamed El Zowalaty, Mohd Zobir Hussein, and Maznah Ismail. "Antimicrobial Activity of Hippurate Nanocomposite and Its Cytotoxicity Effect in Combination with Cytarabine against HL-60." Journal of Nanomaterials 2013 (2013): 1–9. http://dx.doi.org/10.1155/2013/843647.

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Hippuric acid (HA) was intercalated into a zinc-layered hydroxide (ZLH) by direct reaction of an aqueous suspension of zinc oxide with an aqueous solution of hippuric acid to obtain hippurate nanocomposite (HAN). Various concentrations of hippuric acid (0.05, 0.2, and 0.4 molar) were used for the synthesis of the nanocomposite. The as-synthesized HAN using 0.2 molar was found to give a well-ordered layered nanocomposite material with an increase in the basal spacing to 21.3 Å which indicated the insertion of hippurate organic moiety into the ZLH interlayers. The cytotoxicity of HAN in combinat

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Polańska, Żaneta, Zuzanna Pietralik-Molińska, Daria Wojciechowska, et al. "The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids." International Journal of Molecular Sciences 22, no. 14 (2021): 7744. http://dx.doi.org/10.3390/ijms22147744.

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Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind genetic material (multiplied positive electric charge) while lowering undesirable effects (the presence of lipids makes the system more like natural membranes). This study aimed to test the ability to bind and release genetic material by lipoplexes based on trimeric surfactants and lipid formulations of d

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Cosenza, Zachary, Raul Astudillo, Peter I. Frazier, Keith Baar, and David E. Block. "Multi-information source Bayesian optimization of culture media for cellular agriculture." Biotechnology and Bioengineering 119, no. 9 (2022): 2447–58. https://doi.org/10.1002/bit.28132.

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Culture media used in industrial bioprocessing and the emerging field of cellular agriculture is difficult to optimize due to the lack of rigorous mathematical models of cell growth and culture conditions, as well as the complexity of the design space. Rapid growth assays are inaccurate yet convenient, while robust measures of cell number can be time-consuming to the point of limiting experimentation. In this study, we optimized a cell culture media with 14 components using a multi-information source Bayesian optimization algorithm that locates optimal media conditions based on an iterative re

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Shtukmaster, Stella, Predrag Ljubuncic, and Arieh Bomzon. "The Effect of an Aqueous Extract ofTeucrium poliumon Glutathione Homeostasis In Vitro: A Possible Mechanism of Its Hepatoprotectant Action." Advances in Pharmacological Sciences 2010 (2010): 1–7. http://dx.doi.org/10.1155/2010/938324.

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Background.Teucrium poliumis used in Arab traditional medicine to treat liver diseases. Glutathione is an important intracellular antioxidant, and intrahepatic glutathione levels are depleted in liver diseases.Hypothesis and Aim. This investigation tested the hypothesis that aqueous extracts ofT. poliummaintains intracellular glutathione levels by augmenting glutathione peroxidase and glutathione reductase activity in cultured hepatocytes.Methods. The effects of increasing concentrations (0.01–1 mg/mL) of aqueous extract ofT. poliumwere assessed in cultured HepG2 cells following 24 hours incub

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Ramos Machado Braga, Jacqueline, Bruna Dos Santos Bispo, Victor Lucas Dias Cavalcante, et al. "VIABILITY OF VERO E6 CELLS EXPOSED TO THE Bothrops leucurus SNAKE CRUDE VENOM." Science And Animal Health 6, no. 3 (2019): 190. http://dx.doi.org/10.15210/sah.v6i3.14231.

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Primary and continuous cell culture systems are a good in vitro alternative for the quantitative assessment of snake venom toxicity. There are still few studies evaluating the cellular response against Bothrops leucurus venom (BlV). This study aimed to evaluate the cytotoxic effect of crude BlV on culture of VERO E6 cells. Neutral Red incorporation (NR) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) tests were performed for viable cells, and Trypan Blue (TB) dye exclusion test was performed for non-viable cells. The differences between the groups were evaluated by ANOV

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Martins, Christine Men, Elizane Ferreira Hamanaka, Thayse Yumi Hoshida, et al. "Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability." Brazilian Dental Journal 27, no. 6 (2016): 751–56. http://dx.doi.org/10.1590/0103-6440201600987.

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Abstract Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to ma

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Abdelfattah, Maha I., Sherine A. Nasry, and Amani A. Mostafa. "Characterization and Cytotoxicity Analysis of a Ciprofloxacin Loaded Chitosan/Bioglass Scaffold on Cultured Human Periodontal Ligament Stem Cells: a Preliminary Report." Open Access Macedonian Journal of Medical Sciences 4, no. 3 (2016): 461–67. http://dx.doi.org/10.3889/oamjms.2016.052.

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AIM: The aim of this study was to analyze the cytotoxicity of ciprofloxacin (CIP) loaded on chitosan bioactive glass scaffold on human periodontal ligament stem cells (PLSCs) in vitro.MATERIALS AND METHODS: PLSCs obtained from human third molars, cultures treated with medium containing 15 x 15 mm chitosan/bioactive glass scaffolds without/with different concentration 0, 5, 10, and 20 % of CIP. A total of 15 x 10^3 cells were plated in 6 well plates. The attached cells of each group were harvested from the plates after 1, 4 and 8 days of culture to detect the viability of cells. The cell number

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