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1

Dale, C., S. C. Welburn, I. Maudlin, and P. J. M. Milligan. "The kinetics of maturation of trypanosome infections in tsetse." Parasitology 111, no. 2 (1995): 187–91. http://dx.doi.org/10.1017/s0031182000064933.

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SUMMARYEstimates of the time delay between the infective bloodmeal and maturation (incubation or maturation time) for 4 trypanosome stocks (2 Trypanozoon and 2 Trypanosoma congolense) show that maturation time in tsetse is not a parasite species-specific constant. The mean incubation time of a Trypanosoma brucei rhodesiense stock (EATRO 2340 – 18 days) was not significantly different from one T. congolense stock (SIKUDA88 – 15·5 days) but was significantly greater than another (1/148 FLY9 – 12·5 days). There was no significant difference in incubation times between male and female Glossina mor
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2

PUMHOM, P., D. POGNON, S. YANGTARA, et al. "Molecular prevalence of Trypanosoma spp. in wild rodents of Southeast Asia: influence of human settlement habitat." Epidemiology and Infection 142, no. 6 (2013): 1221–30. http://dx.doi.org/10.1017/s0950268813002161.

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SUMMARYThis study investigated the molecular prevalence of Trypanosoma lewisi and T. evansi in wild rodents from Cambodia, Lao PDR and Thailand. Between 2008 and 2012, rodents (and shrews) were trapped in nine locations and 616 of these were tested using three sets of primers: TRYP1 (amplifying ITS1 of ribosomal DNA of all trypanosomes), TBR (amplifying satellite genomic DNA of Trypanozoon parasites) and LEW1 (amplifying ITS1 of ribosomal DNA of T. lewisi). Based on the size of the PCR products using TRYP1, 17% were positive for T. lewisi and 1·0% positive for Trypanozoon. Results were confirm
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3

Uilenberg, G. "Nomenclature of trypanosomes of the subgenus Trypanozoon." Transactions of the Royal Society of Tropical Medicine and Hygiene 81, no. 4 (1987): 701. http://dx.doi.org/10.1016/0035-9203(87)90464-0.

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4

Else, Anthony J., James F. Clarke, Anthony Willis, Simon A. Jackman, David W. Hough, and Michael J. Danson. "Dihydrolipoamide dehydrogenase in the Trypanosoma subgenus, Trypanozoon." Molecular and Biochemical Parasitology 64, no. 2 (1994): 233–39. http://dx.doi.org/10.1016/0166-6851(93)00016-3.

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5

Gibson, W. C., P. Dukes, and J. K. Gashumba. "Species-specific DNA probes for the identification of African trypanosomes in tsetse flies." Parasitology 97, no. 1 (1988): 63–73. http://dx.doi.org/10.1017/s0031182000066749.

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SUMMARYWe have obtained 5 specific DNA probes for African trypanosomes of the subgenera Trypanozoon and Nannomonas. Each probe consists of one repeat unit of the major repetitive DNA (satellite DNA) of each species or intra-specific group. One probe hybridized with all members of subgenus Trypanozoon (except T. equiperdum which was not tested). In subgenus Nannomonas, one probe recognized T. simiae, but 3 probes were needed to identify all stocks of T. congolense available. Each of the 3 latter probes recognized trypanosomes from one of the 3 major groups of T. congolense previously defined by
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6

Stevens, J. R., and D. G. Godfrey. "Numerical taxonomy of Trypanozoon based on polymorphisms in a reduced range of enzymes." Parasitology 104, no. 1 (1992): 75–86. http://dx.doi.org/10.1017/s0031182000060820.

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SUMMARYNumerical analyses of Trypanozoon taxonomy are presented, based on the isoenzyme data of Stevens et al. (1992). The previous study used a reduced range of enzymes compared with earlier work; the analyses indicate the value of this rationalized system. Both recently isolated trypanosome stocks and previously studied populations were included, allowing detailed comparison with earlier studies. Relationships between zymodemes were calculated with an improved similarity coefficient program, using Jaccard's coefficient (1908), and by Nei's method (1972). Dendrograms were constructed from the
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7

Cauchard, S., N. Van Reet, P. Büscher, et al. "Killing of Trypanozoon Parasites by the Equine Cathelicidin eCATH1." Antimicrobial Agents and Chemotherapy 60, no. 5 (2016): 2610–19. http://dx.doi.org/10.1128/aac.01127-15.

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ABSTRACTTrypanozoonparasites infect both humans, causing sleeping sickness, and animals, causing nagana, surra, and dourine. Control of nagana and surra depends to a great extent on chemotherapy. However, drug resistance to several of the front-line drugs is rising. Furthermore, there is no official treatment for dourine. Therefore, there is an urgent need to develop antiparasitic agents with novel modes of action. Host defense peptides have recently gained attention as promising candidates. We have previously reported that one such peptide, the equine antimicrobial peptide eCATH1, is highly a
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8

Gibson, Wendy C., Klaas A. Osinga, Paul A. M. Michels, and Piet Borst. "Trypanosomes of subgenus trypanozoon are diploid for housekeeping genes." Molecular and Biochemical Parasitology 16, no. 3 (1985): 231–42. http://dx.doi.org/10.1016/0166-6851(85)90066-0.

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9

Misra, K. K., S. Roy, and A. Choudhury. "Biology of Trypanosoma (Trypanozoon) evansi in experimental heterologous mammalian hosts." Journal of Parasitic Diseases 40, no. 3 (2015): 1047–61. http://dx.doi.org/10.1007/s12639-014-0633-1.

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10

Vourchakbé, Joël, Zebaze Arnol Auvaker Tiofack, Tagueu Sartrien Kante, Mbida Mpoame, and Gustave Simo. "Molecular identification of Trypanosoma brucei gambiense in naturally infected pigs, dogs and small ruminants confirms domestic animals as potential reservoirs for sleeping sickness in Chad." Parasite 27 (2020): 63. http://dx.doi.org/10.1051/parasite/2020061.

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Human African trypanosomiasis (HAT) has been targeted for zero transmission to humans by 2030. Animal reservoirs of gambiense-HAT could jeopardize these elimination goals. This study was undertaken to identify potential host reservoirs for Trypanosoma brucei gambiense by detecting its natural infections in domestic animals of Chadian HAT foci. Blood samples were collected from 267 goats, 181 sheep, 154 dogs, and 67 pigs. Rapid diagnostic test (RDT) and capillary tube centrifugation (CTC) were performed to search for trypanosomes. DNA was extracted from the buffy coat, and trypanosomes of the s
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11

Welburn, S. C., I. Maudlin, and P. J. M. Milligan. "Trypanozoon: Infectivity to Humans Is Linked to Reduced Transmissibility in Tsetse." Experimental Parasitology 81, no. 3 (1995): 404–8. http://dx.doi.org/10.1006/expr.1995.1131.

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12

Milligan, P. J. M., I. Maudlin, and S. C. Welburn. "Trypanozoon: Infectivity to Humans Is Linked to Reduced Transmissibility in Tsetse." Experimental Parasitology 81, no. 3 (1995): 409–15. http://dx.doi.org/10.1006/expr.1995.1132.

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13

Cronin, C. N., and K. F. Tipton. "Purification and regulatory properties of phosphofructokinase from Trypanosoma (Trypanozoon) brucei brucei." Biochemical Journal 227, no. 1 (1985): 113–24. http://dx.doi.org/10.1042/bj2270113.

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Phosphofructokinase (EC 2.7.1.11) from Trypanosoma (Trypanozoon) brucei brucei was purified to homogeneity by using a three-step procedure that may be performed within 1 day. Proteolysis, which removes a fragment of Mr approx. 2000, may occur during the purification, but this can be prevented by including antipain, an inhibitor of cysteine proteinases, in the buffers during the purification. The subunits of the enzyme appear to be identical in size, with an Mr of 49 000. The Mr of the native enzyme was estimated to be approx. 220 000, suggesting a tetrameric structure. Kinetic studies showed t
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14

Masiga, Daniel K., and Wendy C. Gibson. "Specific probes for Trypanosoma (Trypanozoon) evansi based on kinetoplast DNA minicircles." Molecular and Biochemical Parasitology 40, no. 2 (1990): 279–83. http://dx.doi.org/10.1016/0166-6851(90)90049-r.

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15

Biteau, N., F. Bringaud, W. Gibson, P. Truc, and T. Baltz. "Characterization of Trypanozoon isolates using a repeated coding sequence and microsatellite markers." Molecular and Biochemical Parasitology 105, no. 2 (2000): 187–202. http://dx.doi.org/10.1016/s0166-6851(99)00171-1.

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16

Biswas, Debjani, A. Choudhury, and K. K. Misra. "Histopathology of Trypanosoma (Trypanozoon) evansi Infection in Bandicoot Rat. I. Visceral Organs." Experimental Parasitology 99, no. 3 (2001): 148–59. http://dx.doi.org/10.1006/expr.2001.4664.

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17

Filgueiras, Alessandra, Juliana Helena da Silva Barros, Samanta C. C. Xavier, et al. "Natural Trypanosoma (Trypanozoon) evansi (Steel, 1885) infection among mammals from Brazilian Amazon." Acta Tropica 190 (February 2019): 92–98. http://dx.doi.org/10.1016/j.actatropica.2018.11.011.

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18

Asonganyi, Tazoacha, S. Suh, and M. D. Tetuh. "Prévalence des trypanosomoses des animaux domestiques dans le foyer de la maladie du sommeil de Fontem au Cameroun." Revue d’élevage et de médecine vétérinaire des pays tropicaux 43, no. 1 (1990): 69–74. http://dx.doi.org/10.19182/remvt.8900.

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Les auteurs ont examiné, dans le foyer de la maladie du sommeil de Fontem (Cameroun), 304 animaux domestiques dont 114 chèvres, 93 moutons, 67 chiens et 30 porcs, pour la détection de la trypanosomose, à l'aide des tests parasitologiques et sérologiques. Le sous-genre Nannomonas a été le seul détecté chez les animaux, avec une prévalence de 28,3 p. 100. Le test d'agglutination sur carte (Testryp CATT) a montré un taux de positivité de 38,2 p. 100, ce qui a permis une meilleure estimation de la trypanosomose animale. L'absence des trypanosomes du sous-genre Trypanozoon indique probablement que
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19

Al-Taqi, Muna M. "Characterization of trypanosoma (Trypanozoon) evansi from dromedary camels in Kuwait by isoenzyme electrophoresis." Veterinary Parasitology 32, no. 2-3 (1989): 247–53. http://dx.doi.org/10.1016/0304-4017(89)90124-6.

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20

Majiva, P. A. O., R. Hamers, N. Van Meirvenne, and G. Matthyssens. "Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense." Parasitology 93, no. 2 (1986): 291–304. http://dx.doi.org/10.1017/s0031182000051465.

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SUMMARYGenetic proximity between two karyotypic groups of Trypanosoma congolense was investigated using as hybridization probes: (i) total genomic DNA, (ii) a 35 nucleotide long synthetic oligonucleotide, and (iii) non-variant antigen type (non-VAT) specific complementary DNAs. The phylogenetic relationship between Trypanosoma brucei and T. evansi, both of which are accepted species in the subgenus Trypanozoon, was used as a reference to assess the phylogenetic proximity of the two groups of T. congolense. Results indicate that some morphologically indistinguishable T. congolense populations d
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21

ZABLOTSKII, V. T., C. GEORGIU, TH DE WAAL, P. H. CLAUSEN, F. CLAES, and L. TOURATIER. "The current challenges of dourine: difficulties in differentiating Trypanosoma equiperdum within the subgenus Trypanozoon." Revue Scientifique et Technique de l'OIE 22, no. 3 (2003): 1087–96. http://dx.doi.org/10.20506/rst.22.3.1460.

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22

Gibson, W. C., and B. T. Wellde. "Characterization of Trypanozoon stocks from the South Nyanza sleeping sickness focus in Western Kenya." Transactions of the Royal Society of Tropical Medicine and Hygiene 79, no. 5 (1985): 671–76. http://dx.doi.org/10.1016/0035-9203(85)90187-7.

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23

Biswas, Debjani, Amalesh Choudhury, and Kamales Kumar Misra. "Histopathology of Trypanosoma (Trypanozoon) evansi infection in Bandicoot rat. II. Brain and choroid plexus." Proceedings of the Zoological Society 63, no. 1 (2010): 27–37. http://dx.doi.org/10.1007/s12595-010-0004-6.

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24

Enyaru, J. C. K., J. R. Stevens, M. Odiit, N. M. Okuna, and J. F. Carasco. "Isoenzyme comparison of Trypanozoon isolates from two sleeping sickness areas of south-eastern Uganda." Acta Tropica 55, no. 3 (1993): 97–115. http://dx.doi.org/10.1016/0001-706x(93)90072-j.

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25

Ngeranwa, J. J. N., E. R. Mutiga, G. J. O. Agumbah, P. K. Gathumbi, and W. K. Munyua. "The effects of experimental Trypanosoma (trypanozoon) (brucei) evansi infection on the fertility of male goats." Veterinary Research Communications 15, no. 4 (1991): 301–8. http://dx.doi.org/10.1007/bf00430035.

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26

TRAN, T., F. CLAES, J. C. DUJARDIN, and P. BUSCHER. "The invariant surface glycoprotein ISG75 gene family consists of two main groups in the Trypanozoon subgenus." Parasitology 133, no. 05 (2006): 613. http://dx.doi.org/10.1017/s0031182006000953.

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27

Richardson, Joshua Brian, Kuang-Yao Lee, Paul Mireji, et al. "Genomic analyses of African Trypanozoon strains to assess evolutionary relationships and identify markers for strain identification." PLOS Neglected Tropical Diseases 11, no. 9 (2017): e0005949. http://dx.doi.org/10.1371/journal.pntd.0005949.

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28

Claes, F., S. Deborggraeve, D. Verloo, et al. "Validation of a PCR-Oligochromatography Test for Detection of Trypanozoon Parasites in a Multicenter Collaborative Trial." Journal of Clinical Microbiology 45, no. 11 (2007): 3785–87. http://dx.doi.org/10.1128/jcm.01244-07.

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29

Witola, William H., Nopporn Sarataphan, Noboru Inoue, Kazuhiko Ohashi, and Misao Onuma. "Genetic variability in ESAG6 genes among Trypanosoma evansi isolates and in comparison to other Trypanozoon members." Acta Tropica 93, no. 1 (2005): 63–73. http://dx.doi.org/10.1016/j.actatropica.2004.09.006.

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30

Mulla, A. F., and L. R. Rickman. "The isolation of human serum-resistant Trypanosoma (Trypanozoon) species from zebra and impala in Luangwa Valley, Zambia." Transactions of the Royal Society of Tropical Medicine and Hygiene 82, no. 5 (1988): 718. http://dx.doi.org/10.1016/0035-9203(88)90211-8.

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31

Gibson, Wendy C., and Piet Borst. "Size-fractionation of the small chromosomes of Trypanozoon and Nannomonas trypanosomes by pulsed field gradient gel electrophoresis." Molecular and Biochemical Parasitology 18, no. 2 (1986): 127–40. http://dx.doi.org/10.1016/0166-6851(86)90033-2.

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32

Nunes, V. L. B., and E. T. Oshiro. "Trypanosoma (Trypanozoon) evansi in the coati from the Pantanal region of Mato Grosso do Sul State, Brazil." Transactions of the Royal Society of Tropical Medicine and Hygiene 84, no. 5 (1990): 692. http://dx.doi.org/10.1016/0035-9203(90)90148-8.

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33

Wang, Ming-Hui, Yan-Zi Wen, Ying Wei, et al. "Mitogen-activated protein kinase 5, a novel molecular marker for the identification and detection of Trypanozoon species." Acta Tropica 122, no. 2 (2012): 183–88. http://dx.doi.org/10.1016/j.actatropica.2012.01.009.

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34

Richner, D., J. Schweizer, B. Betschart, and L. Jenni. "Characterization of West AfricanTrypanosoma (Trypanozoon) brucei isolates from man and animals using isoenzyme analysis and DNA hybridization." Parasitology Research 76, no. 1 (1989): 80–85. http://dx.doi.org/10.1007/bf00931077.

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35

MORLAIS, I., P. GREBAUT, J. M. BODO, S. DJOHA, and G. CUNY. "Characterization of trypanosome infections by polymerase chain reaction (PCR) amplification in wild tsetse flies in Cameroon." Parasitology 116, no. 6 (1998): 547–54. http://dx.doi.org/10.1017/s0031182098002625.

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The polymerase chain reaction (PCR) method was used to characterize trypanosome infections in tsetse flies from 3 sleeping sickness foci in Cameroon. The predominant tsetse species found was Glossina palpalis palpalis. An average infection rate of 12·1% was revealed by microscopical examination of 888 non-teneral tsetse flies. PCR amplification analyses for trypanosome identification were carried out on 467 flies, with primer sets specific for Trypanosoma (Trypanozoon) brucei s.l., T. (Duttonella) vivax, T. (Nannomonas) simiae and forest type T. (Nannomonas) congolense. Of 467 flies 93 were po
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36

HAGOS, A., G. DEGEFA, H. YACOB, V. BONAZZA, and E. BROCCHI. "Seroepidemiological survey of trypanozoon infection in horses in the suspected dourine-infected Bale Highlands of the Oromia region, Ethiopia." Revue Scientifique et Technique de l'OIE 29, no. 3 (2010): 649–54. http://dx.doi.org/10.20506/rst.29.3.2005.

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37

CLAES, F., E. C. AGBO, M. RADWANSKA, et al. "How does Trypanosoma equiperdum fit into the Trypanozoon group? A cluster analysis by RAPD and Multiplex-endonuclease genotyping approach." Parasitology 126, no. 5 (2003): 425–31. http://dx.doi.org/10.1017/s0031182003002968.

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The pathogenic trypanosomes Trypanosoma equiperdum, T. evansi as well as T. brucei are morphologically identical. In horses, these parasites are considered to cause respectively dourine, surra and nagana. Previous molecular attempts to differentiate these species were not successful for T. evansi and T. equiperdum; only T. b. brucei could be differentiated to a certain extent. In this study we analysed 10 T. equiperdum, 8 T. evansi and 4 T. b. brucei using Random Amplified Polymorphic DNA (RAPD) and multiplex-endonuclease fingerprinting, a modified AFLP technique. The results obtained confirm
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38

Hilali, M., and M. M. Fahmy. "Trypanozoon-like epimastigotes in the larvae of Cephalopina titillator (Diptera: Oestridae) infesting camels (Camelus dromedarius) infected with Trypanosoma evansi." Veterinary Parasitology 45, no. 3-4 (1993): 327–29. http://dx.doi.org/10.1016/0304-4017(93)90087-4.

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39

Szpeiter, Bruno Bernal, Juliana Isabel Giuli da Silva Ferreira, Francisco Flávio Vieira de Assis, et al. "Bat trypanosomes from Tapajós-Arapiuns Extractive Reserve in Brazilian Amazon." Revista Brasileira de Parasitologia Veterinária 26, no. 2 (2017): 152–58. http://dx.doi.org/10.1590/s1984-29612017022.

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Abstract Trypanosoma comprises flagellates able to infect several mammalian species and is transmitted by several groups of invertebrates. The order Chiroptera can be infected by the subgenera Herpetosoma, Schizotrypanum, Megatrypanum and Trypanozoon. In this study, we described the diversity of bat trypanosomes and inferred phylogenetic relationships among the trypanosomes from bats caught in Tapajós-Arapiuns Extractive Reserve (Resex) in Pará state. Trypanosomes from bats were isolated by means of hemoculture, and the molecular phylogeny was based on the trypanosome barcode (SSUrDNA V7V8 var
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40

Njiru, Z. K., A. S. J. Mikosza, E. Matovu, et al. "African trypanosomiasis: Sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA." International Journal for Parasitology 38, no. 5 (2008): 589–99. http://dx.doi.org/10.1016/j.ijpara.2007.09.006.

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Li, Feng-Jun, Robin B. Gasser, Jia-Yu Zheng, Filip Claes, Xing-Quan Zhu, and Zhao-Rong Lun. "Application of multiple DNA fingerprinting techniques to study the genetic relationships among three members of the subgenus Trypanozoon (Protozoa: Trypanosomatidae)." Molecular and Cellular Probes 19, no. 6 (2005): 400–407. http://dx.doi.org/10.1016/j.mcp.2005.07.002.

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42

Moreno, S. Andrea, and Gabriela V. Cantos. "The kinetic properties of hexokinases in African trypanosomes of the subgenus Trypanozoon match the blood glucose levels of mammal hosts." Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 217 (March 2018): 51–59. http://dx.doi.org/10.1016/j.cbpb.2017.12.014.

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43

BLACK, S. J., C. N. SENDASHONGA, P. WEBSTER, G. L. E. KOCH, and S. Z. SHAPIRO. "Regulation of parasite-specific antibody responses in resistant (C57BL/6) and susceptible (C3H/HE) mice infected with Trypanosoma (trypanozoon) brucei brucei." Parasite Immunology 8, no. 5 (1986): 425–42. http://dx.doi.org/10.1111/j.1365-3024.1986.tb00859.x.

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44

Enyaru, J. C. K., E. Matovu, M. Odiit, L. A. Okedi, A. J. J. Rwendeire, and J. R. Stevens. "Genetic diversity in Trypanosoma (Trypanozoon) brucei isolates from mainland and Lake Victoria island populations in south-eastern Uganda: epidemiological and control implications." Annals of Tropical Medicine & Parasitology 91, no. 1 (1997): 107–13. http://dx.doi.org/10.1080/00034983.1997.11813118.

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45

Schares, G., and D. Mehlitz. "Sleeping sickness in Zaire: a nested polymerase chain reaction improves the identification of Trypanosoma (Trypanozoon) brucei gambiense by specific kinetoplast DNA probes." Tropical Medicine & International Health 1, no. 1 (1996): 59–70. http://dx.doi.org/10.1046/j.1365-3156.1996.d01-11.x.

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46

Mattioli, Raffaele C., O. Jean, and Adrien Marie Gaston Belem. "Incidence de la trypanosomose sur la faune sauvage d'un ranch de gibier au Burkina Faso." Revue d’élevage et de médecine vétérinaire des pays tropicaux 43, no. 4 (1990): 459–64. http://dx.doi.org/10.19182/remvt.8759.

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Le sang de 203 animaux sauvages élevés dans un ranch de gibier au Burkina Faso a été examiné au niveau de la strate leucocytaire après centrifugation en tube capillaire à microhématocrite. Frottis et gouttes épaisses ont été examinés pour chaque animal afin de détecter la présence de Babesia, Anaplasma, Theileria, et de microfilaires. Le taux d'infection et la parasitémie, suivant l'espèce animale, ont été évalués ainsi que le degré d'anémie par la mesure de l'hématocrite. Pour chaque animal, les paramètres suivants ont été notés: espèce, sexe, âge, poids, présence éventuelle de parasites exte
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47

Mohammed, A. Basheer, O. Mohammed Yassir, M. Hassan Mo awia, A. Mohamed, M. Mohamed Ahmed Mohamed, and E. El Rayah Intisar. "Detection of Trypanozoon trypanosomes infections on Glossina fuscipes fuscipes (Diptera: Glossinidae) using polymerase chain reaction (PCR) technique in the Blue Nile State, Sudan." African Journal of Biotechnology 12, no. 1 (2013): 89–95. http://dx.doi.org/10.5897/ajb12.1856.

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Hamilton, P. B., E. R. Adams, I. I. Malele, and W. C. Gibson. "A novel, high-throughput technique for species identification reveals a new species of tsetse-transmitted trypanosome related to the Trypanosoma brucei subgenus, Trypanozoon." Infection, Genetics and Evolution 8, no. 1 (2008): 26–33. http://dx.doi.org/10.1016/j.meegid.2007.09.003.

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Savani, Elisa San Martin Mouriz, Vania Lúcia Brandão Nunes, Eunice Aparecida Bianchi Galati, et al. "Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil." Memórias do Instituto Oswaldo Cruz 100, no. 7 (2005): 739–41. http://dx.doi.org/10.1590/s0074-02762005000700011.

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Elhaig, Mahmoud M., and Nahla H. Sallam. "Molecular survey and characterization of Trypanosoma evansi in naturally infected camels with suspicion of a Trypanozoon infection in horses by molecular detection in Egypt." Microbial Pathogenesis 123 (October 2018): 201–5. http://dx.doi.org/10.1016/j.micpath.2018.07.017.

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