Relevant bibliographies by topics / Tryptic Soy Agar - TSA

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Academic literature on the topic 'Tryptic Soy Agar - TSA'

Author: Grafiati
Published: 4 June 2021
Last updated: 16 February 2022

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Journal articles on the topic "Tryptic Soy Agar - TSA"

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Nurjanna, Nurjanna, and Ahmadirrahman Fajrihanif. "POPULASI DAN PERTUMBUHAN BAKTERI AIR TAMBAK PADA MEDIA TRYPTIC SOY AGAR (TSA) DARI PABRIKAN YANG BERBEDA." Buletin Teknik Litkayasa Akuakultur 8, no. 1 (2016): 71. http://dx.doi.org/10.15578/blta.8.1.2009.71-73.

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Percobaan ini bertujuan untuk melihat populasi bakteri air tambak menggunakanmedia TSA dari 2 (dua) pabrikan yang berbeda. Pelaksanaan percobaan meliputipemilihan media TSA, pembuatan media TSA, dan isolasi bakteri air tambak. Kedua media TSA tersebut masing-masing ditimbang sebanyak 16 g, kemudian dilarutkan dalam 400 mL aquadest steril. Selanjutnya ditambahkan NaCl sebanyak 6 g. Sterilisasi menggunakan autoclave pada suhu 121oC, 1 atm selama 15 menit. Inokulasi sampel air pada kedua media tersebut dilakukan dengan sistem pengenceran menggunakan larutan garam fisiologis (NaCl 0,85%). Hasil pe
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STRANTZ, A. A., and E. A. ZOTTOLA. "A Modified Plating Technique for the Recovery and Enumeration of Stressed Salmonella typhimurium Hf1." Journal of Food Protection 52, no. 10 (1989): 712–14. http://dx.doi.org/10.4315/0362-028x-52.10.712.

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A plating technique that would allow for recovery and enumeration of stressed Salmonella typhimurium was developed. The strain of S. typhimurium used in this study was isolated from an outbreak of salmonellosis caused by the consumption of contaminated pasteurized milk. Cultures propagated for 18 h at 37°C in Tryptic Soy Broth (TSB) were enumerated on Brilliant Green Agar (BGA), Salmonella-Shigella agar (SS), Xylose Lysine Desoxycholate Agar (XLD), and Hektoen Enteric Agar (HE). Recovery of 101 to 102 fewer cells was seen on the selective agars as compared to recovery on Tryptic Soy Agar (TSA)
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SKEENS, JORDAN W., MARTIN WIEDMANN, and NICOLE H. MARTIN. "Spore-Forming Bacteria Associated with Dairy Powders Can Be Found in Bacteriological Grade Agar–Agar Supply." Journal of Food Protection 83, no. 12 (2020): 2074–79. http://dx.doi.org/10.4315/jfp-20-195.

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ABSTRACT Thermophilic spore-forming bacteria are found ubiquitously in natural environments and, therefore, are present in a number of agricultural food products. Spores produced by these bacteria can survive harsh environmental conditions encountered during food processing and have been implicated in food spoilage. During research efforts to develop a standardized method for enumerating spores in dairy powders, the dairy powder–associated thermophilic sporeformer Anoxybacillus flavithermus was discovered growing in uninoculated control plates of tryptic soy agar (TSA) supplemented with 1% (w/
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KOBAYASHI, HIROSHI, TAKAHISA MIYAMOTO, YOSHIKAZU HASHIMOTO, et al. "Identification of Factors Involved in Recovery of Heat-Injured Salmonella Enteritidis." Journal of Food Protection 68, no. 5 (2005): 932–41. http://dx.doi.org/10.4315/0362-028x-68.5.932.

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Proteins and genes involved in the recovery of heat-injured Salmonella Enteritidis were investigated. Salmonella Enter-itidis cells cultured overnight in tryptic soy broth (TSB; nonselective medium) were suspended in citric acid–disodium hydrogen phosphate buffer (pH 6). After heat treatment at 55°C for 15 min, the culturable counts measured by tryptic soy agar (TSA; nonselective medium) decreased from 108 to 107 CFU/ml. On the other hand, culturable counts measured by desoxycholate–hydrogen sulfite–lactose (DHL) agar (selective medium) were decreased from 108 to 104 CFU/ml by the same treatme
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SMITH, AMANDA R., ALYSHA L. ELLISON, AMANDA L. ROBINSON, et al. "Enumeration of Sublethally Injured Escherichia coli O157:H7 ATCC 43895 and Escherichia coli Strain B-41560 Using Selective Agar Overlays versus Commercial Methods." Journal of Food Protection 76, no. 4 (2013): 674–79. http://dx.doi.org/10.4315/0362-028x.jfp-12-363.

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Quality control procedures during food processing may involve direct inoculation of food samples onto appropriate selective media for subsequent enumeration. However, sublethally injured bacteria often fail to grow, enabling them to evade detection and intervention measures and ultimately threaten the health of consumers. This study compares traditional selective and nonselective agar-based overlays versus two commercial systems (Petrifilm and Easygel) for recovery of injured E. coli B-41560 and O157:H7 strains. Bacteria were propagated in tryptic soy broth (TSB), ground beef slurry, and infan
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Dwinanti, Sefti Heza, and ,. Tanbiyaskur. "Modification of non-selective-solid media for aquatic bacteria." Jurnal Akuakultur Indonesia 13, no. 2 (2015): 163. http://dx.doi.org/10.19027/jai.13.163-166.

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<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="Pa2">The objective of this research was to produce an alternative media for aquatic bacteria using fish broth as pepton source and commercial consumption agar as material. This experiment consisted of six treatments; four treatments used fish broth with doses 200 g/L; 400 g/L; 600 g/L and 800 g/L; two treatments as controls which were commercial agar as negative control and tryptic soy agar (TSA) as positive control. The res
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Wu, Jian, Katheryn M. Goodrich, Joseph D. Eifert, et al. "Inhibiting foodborne pathogens Vibrio parahaemolyticus and Listeria monocytogenes using extracts from traditional medicine: Chinese gallnut, pomegranate peel, Baikal skullcap root and forsythia fruit." Open Agriculture 3, no. 1 (2018): 163–70. http://dx.doi.org/10.1515/opag-2018-0017.

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Abstract Foodborne illnesses have been a heavy burden in the United States and globally. Many medicinal herbs have been cultivated in the US and many of which contain antimicrobial compounds with the potential to be used for food preservation. Methanol/water extracts of pomegranate peel (“PP”, Punica Granatum L.), Chinese gallnut (“CG”, Galla chinensis), Forsythia fruit (“FF”, Forsythia suspensa) and Baikal skullcap root (“BS”, Scutellaria baicalensis) were tested for antimicrobial activity using the agar diffusion assay on tryptic soy agar (TSA) and microdilution assay in tryptic soy broth (T
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WU, V. C. H., D. Y. C. FUNG, D. H. KANG, and L. K. THOMPSON. "Evaluation of Thin Agar Layer Method for Recovery of Acid-Injured Foodborne Pathogens†." Journal of Food Protection 64, no. 7 (2001): 1067–71. http://dx.doi.org/10.4315/0362-028x-64.7.1067.

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The thin agar layer (TAL) method of Kang and Fung was used to enumerate acid-injured foodborne pathogens. This method involves overlaying 14 ml of nonselective medium (tryptic soy agar [TSA]) onto a prepoured and solidified pathogen-specific, selective medium in a petri dish. After surface plating, injured cells resuscitated and grew on TSA during the first few hours of incubation; then, the selective agents from the selective medium diffused to the top layer, interacted with the recovered microorganisms, and started to produce typical reactions. Foodborne pathogens were exposed to 2% acetic a
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BACK, KYEONG-HWAN, SANG-OH KIM, KI-HWAN PARK, MYUNG-SUB CHUNG, and DONG-HYUN KANG. "Spray Method for Recovery of Heat-Injured Salmonella Typhimurium and Listeria monocytogenes." Journal of Food Protection 75, no. 10 (2012): 1867–72. http://dx.doi.org/10.4315/0362-028x.jfp-11-512.

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Selective agar is inadequate for supporting recovery of injured cells. During risk assessment of certain foods, both injured and noninjured cells must be enumerated. In this study, a new method (agar spray method) for recovering sublethally heat-injured microorganisms was developed and used for recovery of heat-injured Salmonella Typhimurium and Listeria monocytogenes. Molten selective agar was applied as an overlay to presolidified nonselective tryptic soy agar (TSA) by spray application. Heat-injured cells (55°C for 10 min in 0.1% peptone water or 55°C for 15 min in sterilized skim milk) wer
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Karolenko, Caitlin E., Arjun Bhusal, Dhiraj Gautam, and Peter M. Muriana. "Selenite Cystine Agar for Enumeration of Inoculated Salmonella Serovars Recovered from Stressful Conditions during Antimicrobial Validation Studies." Microorganisms 8, no. 3 (2020): 338. http://dx.doi.org/10.3390/microorganisms8030338.

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Process validation studies often require the inoculation of select foodborne pathogens into targeted foods to determine the lethality of the process or antimicrobial ingredients, and quantitative recovery of surviving inoculum bacteria helps to make those assessments. Such processes introduce various stressors on the inoculated challenge microorganisms whereby traditional selective media are too harsh to enumerate the remaining viable and injured population quantitatively. Innate antibiotic resistance of challenge organisms has often been used to establish simple selective media (i.e., Tryptic
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Dissertations / Theses on the topic "Tryptic Soy Agar - TSA"

1

Sunmonu, Olasunkanmi. "The Sensitivity of Pseudomonas Agar Plaque Assay in the Isolation of Bacteriophage Φ6 in the Environment: A pilot study". 2017. http://scholarworks.gsu.edu/iph_theses/533.

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Background: Bacteriophage Φ6 is a lipid-enveloped dsRNA bacteriophage. The limitations in our knowledge of how this bacteriophage occurs in the environment are limited by non-selective isolation techniques. Research on finding phages in the environment in the past has employed the Double Agar Layer (DAL) plaque assay using Tryptic Soy Agar (TSA), a non-selective media. The bacterial host for bacteriophage Φ6 is Pseudomonas syringae. In this study, we tested Pseudomonas Agar, a selective media that suppresses the growth of bacteria except Pseudomonas species, in the standard double agar layer p
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