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Journal articles on the topic 'Tryptic Soy Agar - TSA'

Author: Grafiati

Published: 4 June 2021

Last updated: 16 February 2022

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1

Nurjanna, Nurjanna, and Ahmadirrahman Fajrihanif. "POPULASI DAN PERTUMBUHAN BAKTERI AIR TAMBAK PADA MEDIA TRYPTIC SOY AGAR (TSA) DARI PABRIKAN YANG BERBEDA." Buletin Teknik Litkayasa Akuakultur 8, no. 1 (2016): 71. http://dx.doi.org/10.15578/blta.8.1.2009.71-73.

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Percobaan ini bertujuan untuk melihat populasi bakteri air tambak menggunakanmedia TSA dari 2 (dua) pabrikan yang berbeda. Pelaksanaan percobaan meliputipemilihan media TSA, pembuatan media TSA, dan isolasi bakteri air tambak. Kedua media TSA tersebut masing-masing ditimbang sebanyak 16 g, kemudian dilarutkan dalam 400 mL aquadest steril. Selanjutnya ditambahkan NaCl sebanyak 6 g. Sterilisasi menggunakan autoclave pada suhu 121oC, 1 atm selama 15 menit. Inokulasi sampel air pada kedua media tersebut dilakukan dengan sistem pengenceran menggunakan larutan garam fisiologis (NaCl 0,85%). Hasil pe

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2

STRANTZ, A. A., and E. A. ZOTTOLA. "A Modified Plating Technique for the Recovery and Enumeration of Stressed Salmonella typhimurium Hf1." Journal of Food Protection 52, no. 10 (1989): 712–14. http://dx.doi.org/10.4315/0362-028x-52.10.712.

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A plating technique that would allow for recovery and enumeration of stressed Salmonella typhimurium was developed. The strain of S. typhimurium used in this study was isolated from an outbreak of salmonellosis caused by the consumption of contaminated pasteurized milk. Cultures propagated for 18 h at 37°C in Tryptic Soy Broth (TSB) were enumerated on Brilliant Green Agar (BGA), Salmonella-Shigella agar (SS), Xylose Lysine Desoxycholate Agar (XLD), and Hektoen Enteric Agar (HE). Recovery of 101 to 102 fewer cells was seen on the selective agars as compared to recovery on Tryptic Soy Agar (TSA)

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3

SKEENS, JORDAN W., MARTIN WIEDMANN, and NICOLE H. MARTIN. "Spore-Forming Bacteria Associated with Dairy Powders Can Be Found in Bacteriological Grade Agar–Agar Supply." Journal of Food Protection 83, no. 12 (2020): 2074–79. http://dx.doi.org/10.4315/jfp-20-195.

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ABSTRACT Thermophilic spore-forming bacteria are found ubiquitously in natural environments and, therefore, are present in a number of agricultural food products. Spores produced by these bacteria can survive harsh environmental conditions encountered during food processing and have been implicated in food spoilage. During research efforts to develop a standardized method for enumerating spores in dairy powders, the dairy powder–associated thermophilic sporeformer Anoxybacillus flavithermus was discovered growing in uninoculated control plates of tryptic soy agar (TSA) supplemented with 1% (w/

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4

KOBAYASHI, HIROSHI, TAKAHISA MIYAMOTO, YOSHIKAZU HASHIMOTO, et al. "Identification of Factors Involved in Recovery of Heat-Injured Salmonella Enteritidis." Journal of Food Protection 68, no. 5 (2005): 932–41. http://dx.doi.org/10.4315/0362-028x-68.5.932.

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Proteins and genes involved in the recovery of heat-injured Salmonella Enteritidis were investigated. Salmonella Enter-itidis cells cultured overnight in tryptic soy broth (TSB; nonselective medium) were suspended in citric acid–disodium hydrogen phosphate buffer (pH 6). After heat treatment at 55°C for 15 min, the culturable counts measured by tryptic soy agar (TSA; nonselective medium) decreased from 108 to 107 CFU/ml. On the other hand, culturable counts measured by desoxycholate–hydrogen sulfite–lactose (DHL) agar (selective medium) were decreased from 108 to 104 CFU/ml by the same treatme

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5

SMITH, AMANDA R., ALYSHA L. ELLISON, AMANDA L. ROBINSON, et al. "Enumeration of Sublethally Injured Escherichia coli O157:H7 ATCC 43895 and Escherichia coli Strain B-41560 Using Selective Agar Overlays versus Commercial Methods." Journal of Food Protection 76, no. 4 (2013): 674–79. http://dx.doi.org/10.4315/0362-028x.jfp-12-363.

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Quality control procedures during food processing may involve direct inoculation of food samples onto appropriate selective media for subsequent enumeration. However, sublethally injured bacteria often fail to grow, enabling them to evade detection and intervention measures and ultimately threaten the health of consumers. This study compares traditional selective and nonselective agar-based overlays versus two commercial systems (Petrifilm and Easygel) for recovery of injured E. coli B-41560 and O157:H7 strains. Bacteria were propagated in tryptic soy broth (TSB), ground beef slurry, and infan

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6

Dwinanti, Sefti Heza, and ,. Tanbiyaskur. "Modification of non-selective-solid media for aquatic bacteria." Jurnal Akuakultur Indonesia 13, no. 2 (2015): 163. http://dx.doi.org/10.19027/jai.13.163-166.

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<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="Pa2">The objective of this research was to produce an alternative media for aquatic bacteria using fish broth as pepton source and commercial consumption agar as material. This experiment consisted of six treatments; four treatments used fish broth with doses 200 g/L; 400 g/L; 600 g/L and 800 g/L; two treatments as controls which were commercial agar as negative control and tryptic soy agar (TSA) as positive control. The res

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7

Wu, Jian, Katheryn M. Goodrich, Joseph D. Eifert, et al. "Inhibiting foodborne pathogens Vibrio parahaemolyticus and Listeria monocytogenes using extracts from traditional medicine: Chinese gallnut, pomegranate peel, Baikal skullcap root and forsythia fruit." Open Agriculture 3, no. 1 (2018): 163–70. http://dx.doi.org/10.1515/opag-2018-0017.

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Abstract Foodborne illnesses have been a heavy burden in the United States and globally. Many medicinal herbs have been cultivated in the US and many of which contain antimicrobial compounds with the potential to be used for food preservation. Methanol/water extracts of pomegranate peel (“PP”, Punica Granatum L.), Chinese gallnut (“CG”, Galla chinensis), Forsythia fruit (“FF”, Forsythia suspensa) and Baikal skullcap root (“BS”, Scutellaria baicalensis) were tested for antimicrobial activity using the agar diffusion assay on tryptic soy agar (TSA) and microdilution assay in tryptic soy broth (T

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8

WU, V. C. H., D. Y. C. FUNG, D. H. KANG, and L. K. THOMPSON. "Evaluation of Thin Agar Layer Method for Recovery of Acid-Injured Foodborne Pathogens†." Journal of Food Protection 64, no. 7 (2001): 1067–71. http://dx.doi.org/10.4315/0362-028x-64.7.1067.

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The thin agar layer (TAL) method of Kang and Fung was used to enumerate acid-injured foodborne pathogens. This method involves overlaying 14 ml of nonselective medium (tryptic soy agar [TSA]) onto a prepoured and solidified pathogen-specific, selective medium in a petri dish. After surface plating, injured cells resuscitated and grew on TSA during the first few hours of incubation; then, the selective agents from the selective medium diffused to the top layer, interacted with the recovered microorganisms, and started to produce typical reactions. Foodborne pathogens were exposed to 2% acetic a

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9

BACK, KYEONG-HWAN, SANG-OH KIM, KI-HWAN PARK, MYUNG-SUB CHUNG, and DONG-HYUN KANG. "Spray Method for Recovery of Heat-Injured Salmonella Typhimurium and Listeria monocytogenes." Journal of Food Protection 75, no. 10 (2012): 1867–72. http://dx.doi.org/10.4315/0362-028x.jfp-11-512.

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Selective agar is inadequate for supporting recovery of injured cells. During risk assessment of certain foods, both injured and noninjured cells must be enumerated. In this study, a new method (agar spray method) for recovering sublethally heat-injured microorganisms was developed and used for recovery of heat-injured Salmonella Typhimurium and Listeria monocytogenes. Molten selective agar was applied as an overlay to presolidified nonselective tryptic soy agar (TSA) by spray application. Heat-injured cells (55°C for 10 min in 0.1% peptone water or 55°C for 15 min in sterilized skim milk) wer

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10

Karolenko, Caitlin E., Arjun Bhusal, Dhiraj Gautam, and Peter M. Muriana. "Selenite Cystine Agar for Enumeration of Inoculated Salmonella Serovars Recovered from Stressful Conditions during Antimicrobial Validation Studies." Microorganisms 8, no. 3 (2020): 338. http://dx.doi.org/10.3390/microorganisms8030338.

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Process validation studies often require the inoculation of select foodborne pathogens into targeted foods to determine the lethality of the process or antimicrobial ingredients, and quantitative recovery of surviving inoculum bacteria helps to make those assessments. Such processes introduce various stressors on the inoculated challenge microorganisms whereby traditional selective media are too harsh to enumerate the remaining viable and injured population quantitatively. Innate antibiotic resistance of challenge organisms has often been used to establish simple selective media (i.e., Tryptic

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11

TETTEH, GLORIA L., and LARRY R. BEUCHAT. "Sensitivity of Acid-Adapted and Acid-Shocked Shigella flexneri to Reduced pH Achieved with Acetic, Lactic, and Propionic Acids." Journal of Food Protection 64, no. 7 (2001): 975–81. http://dx.doi.org/10.4315/0362-028x-64.7.975.

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Survival and growth characteristics of unadapted, acid-adapted, and acid-shocked Shigella flexneri 2a cells in acidified (pH 3.5 to 5.5) tryptic soy broth with 0.25% glucose (TSB) and tryptic soy agar (TSA) were determined. S. flexneri was grown at 37°C for 18 h in tryptic soy broth without glucose (TSBNG) (unadapted) and TSBNG supplemented with 1% glucose (TSBG) (acid-adapted). Cells grown in TSBNG were acid shocked by adjusting 16-h cultures to pH 5.05 ± 0.05 with lactic acid. Cells were then inoculated into TSB acidified with acetic, lactic, or propionic acids to pH 5.5, 4.5, or 3.5 and inc

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12

Kamallia, Sonia, M. Hasbi, and Budijono Budijono. "Isolation and Identification of Biosurfactant Producing Bacteria from Tofu Liquid Waste UD. Dika Putra, Riau Province." Ilmu Perairan (Aquatic Science) 9, no. 1 (2021): 16. http://dx.doi.org/10.31258/jipas.9.1.p.16-22.

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Tofu liquid waste contains high levels of organic matter, especially protein and amino acids. These organic compounds can be proteins, carbohydrates and fats. Most of the bacteria are able to use oil or fat as a source of carbon and energy, bacteria that have this ability are often known as lipolytic bacteria. This study aims to obtain biosurfactant producing bacteria from tofu wastewater. This research was conducted from July - September 2020. The method used in this research is survey method and emulsification method. The media used for bacterial isolation were Tryptic Soy Broth (TSB) and Tr

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13

KANG, DONG-HYUN, and DANIEL Y. C. FUNG. "Thin Agar Layer Method for Recovery of Heat-Injured Listeria monocytogenes." Journal of Food Protection 62, no. 11 (1999): 1346–49. http://dx.doi.org/10.4315/0362-028x-62.11.1346.

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A thin agar layer (TAL) method was developed to recover heat-injured Listeria monocytogenes. Modified Oxford medium (MOX), a selective plating medium, inhibits heat-injured L. monocytogenes from growing, whereas tryptic soy agar (TSA), a nonselective medium, does not. In order to facilitate recovery of heat-injured L. monocytogenes cells while providing selectivity of isolation of L. monocytogenes from other bacteria in the sample, a unique TAL procedure was developed by overlaying 5 ml of nonselective medium (TSA) onto prepoured and solidified MOX medium in an 8.5-cm–diameter petri dish. The

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14

YUSTE, J., and D. Y. C. FUNG. "Inactivation of Listeria monocytogenes Scott A 49594 in Apple Juice Supplemented with Cinnamon." Journal of Food Protection 65, no. 10 (2002): 1663–66. http://dx.doi.org/10.4315/0362-028x-65.10.1663.

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Normal (pH 3.7) and adjusted (pH 5.0) pasteurized apple juice containing cinnamon (0, 0.1, 0.2, and 0.3%) was inoculated with Listeria monocytogenes Scott A 49594 at 104 CFU/ml and stored at 5 and 20°C for 7 days. Counts on tryptic soy agar (TSA), modified Oxford (MOX) medium, and thin agar layer (TAL) were determined at 1 h and 1, 3, and 7 days. The TAL method (MOX medium overlaid with TSA) was used for the recovery of injured cells. In apple juice, both at normal and adjusted pH, with any doses of cinnamon, no L. monocytogenes (a 4.6-log CFU/ml reduction) was detected after 1 h of storage at

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15

RAJKOWSKI, KATHLEEN T., and ROBERT L. DUDLEY. "Use of Selective Media to Recover Salmonella and Vibrio cholerae after Growth in Reconditioned Pork-Processing Wastewater†." Journal of Food Protection 62, no. 7 (1999): 724–30. http://dx.doi.org/10.4315/0362-028x-62.7.724.

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Selective plating media are used for the enumeration and isolation of bacterial pathogens from food and water samples. This study compared the quantitative recovery of Salmonella spp. and Vibrio cholerae grown in nutrient-limited, filter-sterilized, reconditioned wastewater over the temperature range of 4 to 45°C using nonselective and pathogen-specific selective media. Viable Salmonella were enumerated on tryptic soy agar (TSA) and XLT-4, and viable V. cholerae were enumerated on TSA and thiosulfate-citrate-bile-sucrose agar. There was a statistically significant (P &lt; 0.05) higher reco

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16

LAVIERI, NICOLAS A., JOSEPH G. SEBRANEK, JOSEPH C. CORDRAY, et al. "Evaluation of the Thin Agar Layer Method for the Recovery of Pressure-Injured and Heat-Injured Listeria monocytogenes." Journal of Food Protection 77, no. 5 (2014): 828–31. http://dx.doi.org/10.4315/0362-028x.jfp-13-374.

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A sublethally injured bacterial cell has been defined as a cell that survives a stress such as heating, freezing, acid treatment, or other antimicrobial intervention but can repair the cellular damage exerted by the stressor and later regain its original ability to grow. Consequently, sublethally injured cells are not likely to be included in conventional enumeration procedures, which could result in unrealistically low counts unless efforts are made to encourage recovery of the injured cells before enumeration. The objective of this study was to evaluate the use of the thin agar layer (TAL) m

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17

YUSTE, J., and D. Y. C. FUNG. "Inactivation of Salmonella Typhimurium and Escherichia coli O157:H7 in Apple Juice by a Combination of Nisin and Cinnamon." Journal of Food Protection 67, no. 2 (2004): 371–77. http://dx.doi.org/10.4315/0362-028x-67.2.371.

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Pasteurized apple juice with nisin (0, 25, 50, 100, and 200 ppm, wt/vol) and cinnamon (0 and 0.3%, wt/vol) was inoculated with Salmonella Typhimurium and Escherichia coli O157:H7 at 104 CFU/ml and stored at 5 and 20°C. Counts on tryptic soy agar (TSA), selective medium (xylose lysine desoxycholate agar for Salmonella Typhimurium, and MacConkey sorbitol agar for E. coli O157:H7), and thin agar layer (TAL) were determined at 1 h and 1, 3, 7, and 14 days. The TAL method (selective medium overlaid with TSA) was used for recovery of sublethally injured cells. The pathogens were gradually inactivate

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18

Sinaga, Kristina, M. Hasbi, and Eko Purwanto. "Isolation and Identification of Biosurfactant Producing Bacteria from Slaughterhouse Wastewater." Ilmu Perairan (Aquatic Science) 9, no. 1 (2021): 49. http://dx.doi.org/10.31258/jipas.9.1.p.49-55.

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The slaughterhouse wastewater is rich of oil and fat and it may inhabit by biosurfactant producing bacteria. Isolation of the bacteria is needed in order to find out a biosurfactant producing bacteria that can be used to maintain the oil and fat polluted area through bioremediation process. To isolate and identify the biosurfactant-producing bacteria, a study has been conducted on July to September 2020. The samples were obtained from slaughterhouse wastewater at Cipta Karya street Pekanbaru, Riau Province and samplings were conducted three times. The isolation was conducted by TSB (Tryptic So

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19

BOWMAN, LAUREN S., KIM M. WATERMAN, ROBERT C. WILLIAMS, and MONICA A. PONDER. "Inoculation Preparation Affects Survival of Salmonella enterica on Whole Black Peppercorns and Cumin Seeds Stored at Low Water Activity." Journal of Food Protection 78, no. 7 (2015): 1259–65. http://dx.doi.org/10.4315/0362-028x.jfp-14-483.

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Salmonellosis has been increasingly associated with contaminated spices. Identifying inoculation and stabilization methods for Salmonella on whole spices is important for development of validated inactivation processes. The objective of this study was to examine the effects of inoculation preparation on the recoverability of Salmonella enterica from dried whole peppercorns and cumin seeds. Whole black peppercorns and cumin seeds were inoculated with S. enterica using one dry transfer method and various wet inoculation methods: immersion of spice seeds in tryptic soy broth (TSB) plus Salmonella

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20

AHMED, NAHED M., and DONALD E. CONNER. "Evaluation of Various Media for Recovery of Thermally-Injured Escherichia coli O157:H71." Journal of Food Protection 58, no. 4 (1995): 357–60. http://dx.doi.org/10.4315/0362-028x-58.4.357.

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Efficacies of plating media for recovering heated Escherichia coli O157:H7 were determined and compared. To compare populations of recovered cells, suspensions of cells (three isolates, four replications/isolate) were heated at 50, 55, or 60°C, and then inoculated onto eight media: PCA-PA (plate count agar with 1% pyruvic acid [PA]), MSA (MacConkey sorbitol agar), MSA-Mg (MSA with 0.025% MgSO4), MSA-PA (MSA with 1% PA), MSA-MUG (MSA with 0.005% 4-methylumbelliferyl-β-d-glucuronide (MUG), PRSA-MUG (phenol red sorbitol agar [PSRA] with 0.005% MUG), PRSA-PA (PRSA with 1% PA), and TSA-PA (tryptic

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21

CROZIER-DODSON, BETH ANN, and DANIEL Y. C. FUNG. "Comparison of Recovery of Airborne Microorganisms in a Dairy Cattle Facility Using Selective Agar and Thin Agar Layer Resuscitation Media†." Journal of Food Protection 65, no. 9 (2002): 1488–92. http://dx.doi.org/10.4315/0362-028x-65.9.1488.

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Thin agar layer (TAL) medium was developed at Kansas State University to improve the resuscitation of injured cells and has been shown to result in higher recovery than is obtained with selective media alone for cold-, heat-, salt-, and acid-injured cells. The experiment presented here was designed to determine the effectiveness of the TAL method for the recovery of possibly injured organisms from air. Eleven agar media were used for the experiment: tryptic soy agar (TSA), MacConkey sorbitol agar (MSA), TAL-MSA, Baird-Parker (BP) agar, TAL-BP agar, modified Oxford (MOX) agar, TAL-MOX agar, xyl

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22

Chang, Tsung C., Su H. Huang, H. Y. Chao, et al. "Efficacy of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus: Collaborative Study." Journal of AOAC INTERNATIONAL 79, no. 3 (1996): 661–70. http://dx.doi.org/10.1093/jaoac/79.3.661.

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Abstract Fifteen laboratories completed a collaborative study comparing the efficacy of a latex agglutination kit (Aureus Test) with that of AOAC Official Method 987.09 (coagulase test for identification of Staphylococcus aureus). Each laboratory analyzed 240 strains of bacteria, including 160 isolates of S. aureus and 80 isolates of other bacteria. Upon receipt of cultures, collaborators subcultured each isolate on both tryptic soy agar (TSA) and Baird-Parker agar medium (BPA) to determine whether the growth medium has any effect on either method. For cultures grown on TSA, the latex test had

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23

Matsuoka, Hideaki, Koichiro Nakano, Norimasa Takatani, Tomonori Yoshida, Shizunobu Igimi, and Mikako Saito. "Flow Cytometric Method for in situ Preparation of Standard Materials of a Small Defined Number of Microbial Cells with Colony-Forming Potentiality." Journal of AOAC INTERNATIONAL 97, no. 2 (2014): 479–83. http://dx.doi.org/10.5740/jaoacint.13-302.

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Abstract Standard materials of a small defined number of cells with colony-forming potentiality are essential for the rational validation of food microbiological methods. An in situ flow cytometric method using viable staining with 6-carboxyfluorescein diacetate (CFDA) and tryptic soy agar (TSA) was previously proposed and its feasibility was demonstrated with five strains. In this study, this method was applied to 16 strains to support its broad applicability. The cellsorting gate was previously determined based on the CFDA stainability alone. Now the structural properties of cells designated

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Lee, K. K., K. C. Yii, T. I. Yang, H. I. Hong, and P. C. Liu. "Protease and Virulence of the Extracellular Products Produced by Vibrio carchariae after Growth on Various Media." Zeitschrift für Naturforschung C 54, no. 5-6 (1999): 383–86. http://dx.doi.org/10.1515/znc-1999-5-613.

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Abstract Protease and virulence of the extracellular products (ECP) of Vibrio carchariae strain EmI82KL, a causative agent of gastroenteritis in E pinephelus coioides, cultured on different media were studied. The bacteria grown on peptone agar, nutrient agar or brain heart infusion agar produced higher protease activities than that grown on tryptic soy agar (TSA) in terms of protein content. The addition of ethylenediamine di(o-hydroxyphenylacetic acid) or horse serum in TSA enhanced the ECP protease production while the addition of grouper serum apparently reduced the enzyme activity indica

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LEE, RUBY M., and PAUL A. HARTMAN. "Optimal Pyruvate Concentration for the Recovery of Conforms from Food and Water." Journal of Food Protection 52, no. 2 (1989): 119–21. http://dx.doi.org/10.4315/0362-028x-52.2.119.

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Recoveries of coliforms in water and food samples were increased by adding sodium pyruvate to the Tryptic Soy Agar (TSA) base layer, and Violet Red Bile (VRBA) overlay, of the Modified VRBA procedure described in Standard Methods for the Examination of Dairy Products. Six pyruvate levels (0, 0.005, 0.01, 0.05, 0.1, and 1.0%) were tested. Counts were significantly lower (P≤0.05) on media containing 0% and 1.0% pyruvate than on the other media. Although 0.05% yielded the highest counts overall, there were no significant differences (P≤0.05) among 0.005, 0.01, 0.05, and 0.1% pyruvate. Analysis by

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HVIZDZAK, A. L., S. BEAMER, J. JACZYNSKI, and K. E. MATAK. "Use of Electron Beam Radiation for the Reduction of Salmonella enterica Serovars Typhimurium and Tennessee in Peanut Butter." Journal of Food Protection 73, no. 2 (2010): 353–57. http://dx.doi.org/10.4315/0362-028x-73.2.353.

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Peanut butter and peanut paste products were implicated as the vehicle of contamination in an outbreak of Salmonella Typhimurium, which began in September 2008, and in the November 2006 outbreak of Salmonella Tennessee. Therefore, this study evaluated the effectiveness of electron beam (e-beam) radiation for the reduction of Salmonella serovars Tennessee (ATCC 10722) and Typhimurium (ATCC 14028) in creamy peanut butter. Each strain was studied independently. Peanut butter samples were inoculated with approximately 8.0 log CFU/g of Salmonella, and exposed to e-beam doses ranging from 0 to 3.1 k

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RYU, JEE-HOON, YUN DENG, and LARRY R. BEUCHAT. "Behavior of Acid-Adapted and Unadapted Escherichia coli O157:H7 When Exposed to Reduced pH Achieved with Various Organic Acids." Journal of Food Protection 62, no. 5 (1999): 451–55. http://dx.doi.org/10.4315/0362-028x-62.5.451.

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A study was done to determine if various organic acids differ in their inhibitory or lethal activity against acid-adapted and unadapted Escherichia coli O157:H7 cells. E. coli O157:H7 strain E0139, isolated from venison jerky, was grown in tryptic soy broth (TSB) and in TSB supplemented with 1% glucose (TSBG) for 18 h at 37°C, then plated on tryptic soy agar (TSA) acidified with malic, citric, lactic, or acetic acid at pH 5.4, 5.1, 4.8, 4.5, 4.2, and 3.9. Regardless of whether cells were grown in TSB or TSBG, visible colonies were not formed when plated on TSA acidified with acetic, lactic, ma

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ENACHE, ELENA, AI KATAOKA, D. GLENN BLACK, CARLA D. NAPIER, RICHARD PODOLAK, and MELINDA M. HAYMAN. "Development of a Dry Inoculation Method for Thermal Challenge Studies in Low-Moisture Foods by Using Talc as a Carrier for Salmonella and a Surrogate (Enterococcus faecium)." Journal of Food Protection 78, no. 6 (2015): 1106–12. http://dx.doi.org/10.4315/0362-028x.jfp-14-396.

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The objective of this study was to obtain dry inocula of Salmonella Tennessee and Enterococcus faecium, a surrogate for thermal inactivation of Salmonella in low-moisture foods, and to compare their thermal resistance and stability over time in terms of survival. Two methods of cell growth were compared: cells harvested from a lawn on tryptic soy agar (TSA-cells) and from tryptic soy broth (TSB-cells). Concentrated cultures of each organism were inoculated onto talc powder, incubated at 35°C for 24 h, and dried for additional 24 h at room temperature (23 ± 2°C) to achieve a final water activit

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Viegas, Carla, Marta Dias, Beatriz Almeida, et al. "Loading Rates of Dust and Bioburden in Dwellings in an Inland City of Southern Europe." Atmosphere 12, no. 3 (2021): 378. http://dx.doi.org/10.3390/atmos12030378.

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Sampling campaigns indoors have shown that occupants exposed to contaminated air generally exhibit diverse health outcomes. This study intends to assess the deposition rates of total settleable dust and bioburden in the indoor air of dwellings onto quartz fiber filters and electrostatic dust collectors (EDCs), respectively. EDC extracts were inoculated onto malt extract agar (MEA) and dichloran glycerol (DG18) agar-based media used for fungal contamination characterization, while tryptic soy agar (TSA) was applied for total bacteria assessment, and violet red bile agar (VRBA) for Gram-negative

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Taufik, Pipik, and Dayat Bastiawan. "SUSCEPTIBILY OF Aeromonar hydrophylla BACTERIA ISOLATED FROM UNHEALTHY BAUNG FISH (Mystus nemurus) TOWARDS ANTIMICROBIAL AGENTS." Indonesian Journal of Chemistry 3, no. 3 (2010): 166–68. http://dx.doi.org/10.22146/ijc.21883.

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The susceptibility of Aeromonas hydrophila isolated from ikan baung (Mystus nemurus) to antimicrobial agents was carried out. The objective of study is to know the kind of antimicrobial agents for the control aeromonasis of Mystus nemurus. Tryptic soy agar (TSA) plate consisted 106 cells/plate of A. hydrophila on its surface was kept paper disk consisted antimicrobial agent, than incubated on 30 0C for 24 hours. The results showed that from 10 isolates to be tested, 5 isolates were resistant, 2 isolates were intermediate and 3 isolates were sensitive to tetracycline and chloramphenicol respect

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Topalcengiz, Zeynal, Sefa Işık, and Yusuf Alan. "Aside Adapte ve Adapte Olmayan Durağan Faz Escherichia coli O157:H7, Salmonella enterica Typhimurium ve Listeria monocytogenes’in Nar Suyundaki Termal Direnci." Turkish Journal of Agriculture - Food Science and Technology 7, no. 7 (2019): 1000. http://dx.doi.org/10.24925/turjaf.v7i7.1000-1007.2483.

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The purpose of this study was to investigate the thermal resistance of acid adapted and non-adapted stationary phase Escherichia coli O157:H7, Salmonella enterica Typhimurium and Listeria monocytogenes in pomegranate juice. In addition, the performance of generic E. coli was evaluated as an indicator. Non-adapted stationary phase cells were grown by incubating inoculated tryptic soy broth without glucose (TSB-NG) at 36±1°C for 18±2 hours. Tryptic soy broth with 1% glucose (10 g/l; TSBG) was used for acid adaptation. All media used for L. monocytogenes was supplemented with 0.6% yeast extract.

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BURNHAM, JENNIFER A., PATRICIA A. KENDALL, and JOHN N. SOFOS. "Ascorbic Acid Enhances Destruction of Escherichia coli O157:H7 during Home-Type Drying of Apple Slices." Journal of Food Protection 64, no. 8 (2001): 1244–48. http://dx.doi.org/10.4315/0362-028x-64.8.1244.

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Destruction of Escherichia coli O157:H7 was evaluated on inoculated apple slices dehydrated at two temperatures with and without application of predrying treatments. Half-ring slices (0.6 cm thick) of peeled and cored Gala apples were inoculated by immersion for 30 min in a four-strain composite inoculum of E. coli O157:H7. The inoculated slices (8.7 to 9.4 log CFU/g) either received no predrying treatment (control), were soaked for 15 min in a 3.4% ascorbic acid solution, or were steam blanched for 3 min at 88°C immediately prior to drying at 57.2 or 62.8°C for up to 6 h. Samples were plated

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Stackhouse, Robert R., Nancy G. Faith, Charles W. Kaspar, Charles J. Czuprynski, and Amy C. Lee Wong. "Survival and Virulence of Salmonella enterica Serovar Enteritidis Filaments Induced by Reduced Water Activity." Applied and Environmental Microbiology 78, no. 7 (2012): 2213–20. http://dx.doi.org/10.1128/aem.06774-11.

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ABSTRACTSalmonella entericaserovar Enteritidis strain E40 filaments were developed under conditions of a reduced water activity (aw) of 0.95 in tryptic soy broth (TSB) or tryptic soy agar (TSA) supplemented with 8% or 7% NaCl, respectively. Filament formation was accompanied by an increase of biomass without an increase in CFU and was affected by incubation temperature and the physical milieu. The greatest amount of filaments was recovered from TSA with 7% NaCl and incubation at 30°C. Within 2 h of transfer to fresh TSB, filaments started to septate into normal-sized cells, resulting in a rapi

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WILLIAMS, ROBERT C., SUSAN S. SUMNER, and DAVID A. GOLDEN. "Survival of Escherichia coli O157:H7 and Salmonella in Apple Cider and Orange Juice as Affected by Ozone and Treatment Temperature." Journal of Food Protection 67, no. 11 (2004): 2381–86. http://dx.doi.org/10.4315/0362-028x-67.11.2381.

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Inactivation of Escherichia coli O157:H7 and Salmonella in apple cider and orange juice treated with ozone was evaluated. A five-strain mixture of E. coli O157:H7 or a five-serovar mixture of Salmonella was inoculated (7 log CFU/ml) into apple cider and orange juice. Ozone (0.9 g/h) was pumped into juices maintained at 4°C, ambient temperature (approximately 20°C), and 50°C for up to 240 min, depending on organism, juice, and treatment temperature. Samples were withdrawn, diluted in 0.1% peptone water, and surface plated onto recovery media. Recovery of E. coli O157:H7 was compared on tryptic

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WUYTACK, ELKE Y., L. DUONG THI PHUONG, A. AERTSEN, et al. "Comparison of Sublethal Injury Induced in Salmonella enterica Serovar Typhimurium by Heat and by Different Nonthermal Treatments." Journal of Food Protection 66, no. 1 (2003): 31–37. http://dx.doi.org/10.4315/0362-028x-66.1.31.

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We have studied sublethal injury in Salmonella enterica serovar Typhimurium caused by mild heat and by different emerging nonthermal food preservation treatments, i.e., high-pressure homogenization, high hydrostatic pressure, pulsed white light, and pulsed electric field. Sublethal injury was determined by plating on different selective media, i.e., tryptic soy agar (TSA) plus 3% NaCl, TSA adjusted to pH 5.5, and violet red bile glucose agar. For each inactivation technique, at least five treatments using different doses were applied in order to cover an inactivation range of 0 to 5 log units.

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URKU, C. "Isolation and characterization of Pseudomonas putida caused granulomas in cultured sea bass (Dicentrarchus labrax) in Turkey." Journal of the Hellenic Veterinary Medical Society 72, no. 1 (2021): 2661. http://dx.doi.org/10.12681/jhvms.26748.

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The present study describes a Pseudomas infection in diseased European sea bass (Dicentrarchus labrax) caused by Pseudomonas putida in Turkey. Detected symptoms in the diseased fish were internally white nodules in the liver and kidney. Bacteriological samples from the kidney, spleen, liver and blood were streaked onto Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA) containing 1.5 % NaCl. After incubation, bacterial colonies produced florescent pigment under the ultraviolet light were observed. The morphological and physiological characteristics of bacterial colonies were determine

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Guerreiro-Tanomaru, Juliane Maria, Renata Dornelles Morgental, Norberto Batista Faria-Junior, Fábio Luis Camargo Vilela Berbert, and Mário Tanomaru-filho. "Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants." Brazilian Dental Journal 22, no. 4 (2011): 285–87. http://dx.doi.org/10.1590/s0103-64402011000400004.

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This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2

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R. Wahid Nur Yusuf, Rahayu Kusdarwati, Kismiyati, Sri Subekti,. "Isolasi Dan Identifikasi Bakteri Gram Negatif Pada Luka Ikan Maskoki (Carassius auratus) Akibat Infestasi Ektoparasit Argulus sp. [Isolation And Identification Gram Negative Bacteria At Lessions Of Gold Fish (Carassius auratus) By Infestation Ectoparasite Argulus sp." Jurnal Ilmiah Perikanan dan Kelautan 1, no. 2 (2019): 129. http://dx.doi.org/10.20473/jipk.v1i2.11678.

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AbstractOne of them is the problem of disease Argulus sp. is ektoparasite which often attacked in activity of good conducting of sea water and also freshwater. Argulus sp. attacking at part of fin, husk, gill and entire all its body exterior of him. attacked by fish is Argulus sp. will seen is thin, often rub body and arise red pock which later can generate secondary infection. Utilize to prove the existence of bacterium which emerge effect of lession (secondary infection) which because of bite of Argulus sp. that is by identifying bacterium and biochemical test. Media which used in identifyin

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BROWN, JOSEPH C., and XIUPING JIANG. "Prevalence of Antibiotic-Resistant Bacteria in Herbal Products." Journal of Food Protection 71, no. 7 (2008): 1486–90. http://dx.doi.org/10.4315/0362-028x-71.7.1486.

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The objective of this study was to determine the prevalence of antibiotic-resistant bacteria in various herbal products. Twenty-nine herbal supplements (18 traditional and 11 organic products) were purchased from stores and analyzed microbiologically. Total bacterial counts were determined by pour plate and surface spreading on tryptic soy agar (TSA). Antibiotic-resistant bacteria were enumerated on TSA supplemented with ceftriaxone (64 μg/ml) or tetracycline (16 μg/ml). Total bacterial counts ranged from &lt;5 to 2.9 × 105 CFU/g. Ceftriaxone- and tetracycline-resistant bacteria were detec

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Bal, Amandeep, Richa Anand, Odile Berge, and Christopher P. Chanway. "Isolation and identification of diazotrophic bacteria from internal tissues ofPinus contortaandThuja plicata." Canadian Journal of Forest Research 42, no. 4 (2012): 807–13. http://dx.doi.org/10.1139/x2012-023.

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Lodgepole pine ( Pinus contorta var. latifolia Engelm. ex S. Watson) and western red cedar ( Thuja plicata Donn ex D. Don) are capable of growing in soils that are severely nitrogen-limited. A rarely evaluated but possible nitrogen source for gymnosperms growing at nutrient poor sites is nitrogen fixation by endophytic bacteria. To investigate this possibility, we looked for nitrogen-fixing bacteria in surface-sterilized needle, stem, and root tissues of naturally regenerating lodgepole pine and western red cedar seedlings (2–4 years old) and trees (>20 years old) growing near Williams Lake

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IU, JANET, GAURI S. MITTAL, and MANSEL W. GRIFFITHS. "Reduction in Levels of Escherichia coli O157:H7 in Apple Cider by Pulsed Electric Fields." Journal of Food Protection 64, no. 7 (2001): 964–69. http://dx.doi.org/10.4315/0362-028x-64.7.964.

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Many studies have demonstrated that high voltage pulsed electric field (PEF) treatment has lethal effects on microorganisms including Escherichia coli O157:H7; however, the survival of this pathogen through the PEF treatment is not fully understood. Fresh apple cider samples inoculated with E. coli O157:H7 strain EC920026 were treated with 10, 20, and 30 instant charge reversal pulses at electric field strengths of 60, 70, and 80 kV/cm, at 20, 30, and 42°C. To accurately evaluate the lethality of apple cider processing steps, counts were determined on tryptic soy agar (TSA) and sorbitol MacCon

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LIN, CHIA-MIN, and CHENG-I. WEI. "Transfer of Salmonella montevideo onto the Interior Surfaces of Tomatoes by Cutting†." Journal of Food Protection 60, no. 7 (1997): 858–62. http://dx.doi.org/10.4315/0362-028x-60.7.858.

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Salmonella contamination of precut watermelon, tomatoes, and cantaloupes was responsible for several outbreaks of salmonellosis. To better understand the relationship between bacterial doses and their transfer onto cut surfaces by using a knife, rifampicin-resistant Salmonella montevideo at 7, 70, 700, or 7,000 CFU in Butterfield's buffer (BPB) or tryptic soy broth (TSB) was added to the stem scars of tomatoes. Tomatoes were cut from the stem scar to blossom end using a sterilized knife. After stem scars were aseptically removed, cut surfaces were placed on tryptic soy agar-rifampicin (TSA-RIF

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ADLER, JEREMY M., ERIN D. CAIN-HELFRICH, and CANGLIANG SHEN. "Reductions in Natural Microbial Flora, Nonpathogenic Escherichia coli, and Pathogenic Salmonella on Jalapeno Peppers Processed in a Commercial Antimicrobial Cabinet: A Pilot Plant Trial." Journal of Food Protection 79, no. 11 (2016): 1854–59. http://dx.doi.org/10.4315/0362-028x.jfp-16-222.

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ABSTRACT This experiment aimed to validate the use of antimicrobial solutions in a spray cabinet to inactivate natural microbial flora, nonpathogenic Escherichia coli, and Salmonella on jalapeno peppers. Jalapeno peppers, uninoculated or inoculated with a five-strain mixture of rifampin-resistant E. coli (3.9 log CFU/g) or novobiocin- and nalidixic acid–resistant Salmonella (4.2 log CFU/g), were passed through a commercial antimicrobial cabinet containing both a top and bottom bar spraying (1.38 bar and 2 liters/min) water, sodium hypochlorite (50 ppm), sodium hypochlorite with pH adjusted to

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Aldy Mulyadin, Widanarni Widanarni, Munti Yuhana, and Dinamella Wahjuningrum. "Growth performance, immune response, and resistance of Nile tilapia fed paraprobiotic Bacillus sp. NP5 against Streptococcus agalactiae infection." Jurnal Akuakultur Indonesia 20, no. 1 (2021): 34–46. http://dx.doi.org/10.19027/jai.20.1.34-46.

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ABSTRACT  This study was aimed to evaluate the effectiveness of Bacillus sp. NP5 paraprobiotic administration through commercial feed on growth performance, immune response, and resistance of Nile tilapia against Streptococcus agalactiae infection. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h, then performed a viability test on tryptic soy agar (TSA) media and incubated for 24 hours. Paraprobiotics could be used whether the bacteria did not grow on the TSA media. This study used a completely randomized design, containing three treatments wit

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Kang, David, Joseph D. Eifert, Robert C. Williams, and Steven Pao. "Evaluation of Quantitative Recovery Methods for Listeria monocytogenes Applied to Stainless Steel." Journal of AOAC INTERNATIONAL 90, no. 3 (2007): 810–16. http://dx.doi.org/10.1093/jaoac/90.3.810.

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Abstract The ability of Listeria monocytogenes to attach to various food contact surfaces, such as stainless steel, polypropylene, and rubber compounds, is well documented. The retention of these or other pathogenic bacteria on food contact surfaces increases the risk of transmission to food products. The objective of this study was to compare several methods for quantitative recovery of Listeria monocytogenes from stainless steel surfaces. A cocktail of 4 serotypes of Listeria monocytogenes mixed in equivalent concentrations was inoculated onto type 304 stainless steel coupons in a 2 2 cm are

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CONNER, D. E., and S. F. BILGILI. "Skin Attachment Model for Improved Laboratory Evaluation of Potential Carcass Disinfectants for their Efficacy Against Salmonella Attached to Broiler Skin." Journal of Food Protection 57, no. 8 (1994): 684–88. http://dx.doi.org/10.4315/0362-028x-57.8.684.

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An improved laboratory system for testing the efficacy of potential broiler carcass disinfectants against attached Salmonella was developed. Breast skin from freshly processed chilled broilers was cut into 10 cm diameter pieces, irradiated to inactivate vegetative microflora and served as the attachment surface. Salmonella typhimurium and Salmonella montevideo were grown in brain heart infusion (BHI) broth at 23 or 37°C, diluted in 0.1 M potassium phosphate buffer (KPB), and inoculated onto skin samples as a coarse aerosol spray. At 10, 20 or 30 min post inoculation, skins were vigorously rins

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Fang, Xiao-Mei, Jing Su, Hao Wang, et al. "Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant." International Journal of Systematic and Evolutionary Microbiology 63, Pt_11 (2013): 4158–62. http://dx.doi.org/10.1099/ijs.0.052688-0.

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Two actinobacterial strains, CPCC 203464T and CPCC 203448, isolated from surface-sterilized stems of medicinal plants were subjected to a polyphasic taxonomic study. These two aerobic organisms formed pale yellow colonies on tryptic soy agar (TSA). Cells were Gram-stain-positive, non-acid-fast, non-motile, rod- or coccoid-like elements. Comparative 16S rRNA gene sequence analysis indicated that strains CPCC 203464T and CPCC 203448 were most closely related to the type strains of the species of the genus Williamsia . Chemotaxonomic properties such as containing meso-diaminopimelic acid in the c

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Viegas, Carla, Ana Monteiro, Elisabete Carolino, and Susana Viegas. "Occupational exposure to bioburden in Portuguese bakeries: an approach to sampling viable microbial load." Archives of Industrial Hygiene and Toxicology 69, no. 3 (2018): 250–57. http://dx.doi.org/10.2478/aiht-2018-69-3116.

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AbstractIn bakeries, a number of operations such as mixing are associated with exposure to air-suspended flour dust and related bioburden. The aim of this study was to find the best active sampling approach to the assessment of occupational exposure to bioburden in Portuguese bakeries based on the data obtained with the use of specific impaction and impinger devices. We used impaction to collect fungal particles from 100 L air samples onto malt extract agar (MEA) supplemented with chloramphenicol (0.05 %). For growing fungi we also used dichloran glycerol (DG18) agar-based media and for mesoph

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Stoodley, Paul, Jacob Brooks, Casey W. Peters, et al. "Prevention and Killing Efficacy of Carbapenem Resistant Enterobacteriaceae (CRE) and Vancomycin Resistant Enterococci (VRE) Biofilms by Antibiotic-Loaded Calcium Sulfate Beads." Materials 13, no. 15 (2020): 3258. http://dx.doi.org/10.3390/ma13153258.

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Carbapenem-resistant Enterobacteriaceae (CRE) and vancomycin-resistant Enterococci (VRE) have emerged as multidrug-resistant (MDR) pathogens associated with periprosthetic joint infections (PJI). In this study, we evaluated the efficacy of antibiotic-loaded calcium sulfate beads (ALCSB) in inhibiting bacterial growth, encouraging biofilm formation and killing preformed biofilms of CRE and VRE. Three strains of Klebsiella pneumoniae (KP) and a strain of Enterococcus faecalis (EF) were used. ALCSB of 4.8-mm diameter were loaded with vancomycin (V) and gentamicin (G), V and rifampicin (R), V and

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MADHYASTHA, M. S., R. R. MARQUARDT, A. A. FROHLICH, and J. BORSA. "Optimization of Yeast Bioassay for Trichothecene Mycotoxins." Journal of Food Protection 57, no. 6 (1994): 490–95. http://dx.doi.org/10.4315/0362-028x-57.6.490.

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An improved disc diffusion type bioassay was developed for T-2 toxin (T-2), HT-2 toxin (HT-2), diacetoxyscirpenol (DAS), deoxynivalenol (DON), nivalenol (NIV), neosolaniol (NSL), fusarenon-X (FUS-X), trichothecin (TIN), roridin A (RDA) and verrucarin A (VCA) using the yeast, Kluyveromyces marxianus. Factors such as type of medium, agar volume per plate, preincubation time and temperature, incubation temperature, inoculum size and pH had variable, and in some cases a dramatic effect on the sensitivity of the assay. The effect of pH of the assay medium was particularly pronounced. The highest se

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