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Journal articles on the topic 'Tryptic Soy broth and Glucose broth'

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Published: 5 June 2025

Last updated: 2 August 2025

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1

Lopes, Leonardo Quintana Soares, Pedro Henrique Fortes Guerim, Roberto Christ Vianna Santos, Flavia Kolling Marquezan, and Patrícia Kolling Marquezan. "The influence of different culture media on Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus biofilm formation." Bioscience Journal 39 (August 18, 2023): e39096. http://dx.doi.org/10.14393/bj-v39n0a2023-68631.

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Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are frequently isolated in samples of urinary, blood, intestinal, and respiratory infections, among others. These bacteria are also associated with microbial biofilm formation. E. coli, P. aeruginosa, and S. aureus biofilm infections are particularly hard to manage and often associated with nosocomial problems. This study investigated the influence of different culture media on E. coli, P. aeruginosa, and S. aureus biofilm formation. Bacterial performance was evaluated in brain heart infusion broth, Muel

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Ruchi, Agrawal, Shah Mitesh, Singh Puja, and Ranjan K.P. "Comparison of Efficacy of Biphasic BHI Media, Tryptic Soy Broth and Glucose Broth in Isolating Bacteria through Blood Culture of Suspected Septicemia Patients." International Journal of Pharmaceutical and Clinical Research 15, no. 9 (2023): 1414–19. https://doi.org/10.5281/zenodo.11389933.

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<strong>Introduction: </strong>Blood stream infection (BSI), bacteremia and thereby septicaemia remains one of the most important causes of morbidity and mortality throughout the world. The diagnosis of these infections can be confirmed by blood culture which is the gold standard for the diagnosis. No single blood culture medium or system is capable of detecting all the micro-organisms. Present study compares the efficacy of three culture media for isolation of bacteria through blood culture of suspected septicemia patients. <strong>Material & Methods: </strong>Study was con

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3

EDELSON-MAMMEL, SHARON G., RICHARD C. WHITING, SAM W. JOSEPH, and ROBERT L. BUCHANAN. "Effect of Prior Growth Conditions on the Thermal Inactivation of 13 Strains of Listeria monocytogenes in Two Heating Menstrua." Journal of Food Protection 68, no. 1 (2005): 168–72. http://dx.doi.org/10.4315/0362-028x-68.1.168.

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The thermal tolerance of 13 Listeria monocytogenes strains was tested using a submerged heating coil apparatus. The strains were grown individually for 18 h at 37°C in acidogenic tryptic soy broth (without dextrose) supplemented with 1% glucose and 1% glutamine (TSB+G) or nonacidogenic tryptic soy broth supplemented with 1% glutamine but containing no glucose (dextrose) (TSB−G). The former medium results in cells induced for pH-dependent, stationary-phase acid resistance, whereas the latter medium allows L. monocytogenes to grow to high numbers in the absence of glucose, yielding cells that ar

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TETTEH, GLORIA L., and LARRY R. BEUCHAT. "Sensitivity of Acid-Adapted and Acid-Shocked Shigella flexneri to Reduced pH Achieved with Acetic, Lactic, and Propionic Acids." Journal of Food Protection 64, no. 7 (2001): 975–81. http://dx.doi.org/10.4315/0362-028x-64.7.975.

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Survival and growth characteristics of unadapted, acid-adapted, and acid-shocked Shigella flexneri 2a cells in acidified (pH 3.5 to 5.5) tryptic soy broth with 0.25% glucose (TSB) and tryptic soy agar (TSA) were determined. S. flexneri was grown at 37°C for 18 h in tryptic soy broth without glucose (TSBNG) (unadapted) and TSBNG supplemented with 1% glucose (TSBG) (acid-adapted). Cells grown in TSBNG were acid shocked by adjusting 16-h cultures to pH 5.05 ± 0.05 with lactic acid. Cells were then inoculated into TSB acidified with acetic, lactic, or propionic acids to pH 5.5, 4.5, or 3.5 and inc

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5

Jayalekshmi, Swathy Krishna, Arya Radhakrishnan Krishna, Trisha Mary Pandipilly Antony, and Suganthi Ramasamy. "Isolation of Shewanella putrefaciens GRD 03 from Fish and Explication of Biofilm Adherence Potency on Different Substrates." Journal of Pure and Applied Microbiology 16, no. 1 (2022): 157–66. http://dx.doi.org/10.22207/jpam.16.1.04.

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Foodborne pathogens are the main threat and cause of food poisoning. The majority of food infections have been related to the biofilm formation of foodborne pathogens in the food industry. Shewanella putrefaciens (KX355803, GRD 03), a Gram-negative pathogen isolated from mackerel fish, was identified and recognized as a food spoilage bacterium and a strong biofilm producer. The adhesion or attachment ability of Shewanella putrefaciens was determined on steel, plastic, glass, PVC and wood. NB (Nutrient broth), LB (Luria-Bertani broth), TSB (Tryptic soy broth) and BHI (Brain heart infusion broth

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6

Aroumougame, Sandanakirouchenane. "Studies of Impact on Bioactive Molecules of SB2 Isolate and their PGPR Activities from the Marine Environment." Environment and Ecology 42, no. 2A (2024): 655–65. http://dx.doi.org/10.60151/envec/tuil8884.

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Marine microbes are important sources for recovering bioactive substances. One of the marine isolates, SB2, used a variety of media, with tryptic soy broth showing the best growth and yellow coloring. The largest amount of biomass was produced after 72 hours when the strain SB2 was cultured on glucose, molasses, and peptone, a yeast extract containing carbon and nitrogen sources. Yellow pigment must be extracted using methanol, and the isolate SB2 produced the most of this color. At a concentration of 100 g ml-1 of several bacterial pathogens, the recovered pigment from the SB2 isolate exhibit

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7

Topalcengiz, Zeynal, Sefa Işık, and Yusuf Alan. "Aside Adapte ve Adapte Olmayan Durağan Faz Escherichia coli O157:H7, Salmonella enterica Typhimurium ve Listeria monocytogenes’in Nar Suyundaki Termal Direnci." Turkish Journal of Agriculture - Food Science and Technology 7, no. 7 (2019): 1000. http://dx.doi.org/10.24925/turjaf.v7i7.1000-1007.2483.

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The purpose of this study was to investigate the thermal resistance of acid adapted and non-adapted stationary phase Escherichia coli O157:H7, Salmonella enterica Typhimurium and Listeria monocytogenes in pomegranate juice. In addition, the performance of generic E. coli was evaluated as an indicator. Non-adapted stationary phase cells were grown by incubating inoculated tryptic soy broth without glucose (TSB-NG) at 36±1°C for 18±2 hours. Tryptic soy broth with 1% glucose (10 g/l; TSBG) was used for acid adaptation. All media used for L. monocytogenes was supplemented with 0.6% yeast extract.

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8

Ng, Wenfa. "High Cell Density Cultivation of Escherichia coli DH5α in Shake Flasks with a New Formulated Medium". Biotechnology and Bioprocessing 2, № 10 (2021): 01–11. http://dx.doi.org/10.31579/2766-2314/065.

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High cell density cultivation necessitates cell division and biomass formation, the mechanisms of which remain poorly understood, especially from the cellular energetics perspective. Specifically, the sensing of energy abundance and the channelling of nutritional energy into biomass formation and cell maintenance remains enigmatic at the sensory, effector and decision levels. Thus, optimization of cell growth remains an iterative trial and error process where the principal parameters are growth medium composition and incubation temperature. In this study, a new semidefined formulated medium wa

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9

SIMPSON, CATHERINE A., IFIGENIA GEORNARAS, YOHAN YOON, JOHN A. SCANGA, PATRICIA A. KENDALL, and JOHN N. SOFOS. "Effect of Inoculum Preparation Procedure and Storage Time and Temperature on the Fate of Listeria monocytogenes on Inoculated Salami." Journal of Food Protection 71, no. 3 (2008): 494–501. http://dx.doi.org/10.4315/0362-028x-71.3.494.

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Although dry/semidry fermented sausages are characterized as being of low-to-moderate risk for human listeriosis on a per-serving and per-annum basis, data are lacking relative to the fate of postprocessing Listeria monocytogenes contamination during storage of such products. This study evaluated the effect of inoculum preparation and storage conditions on the fate of L. monocytogenes on vacuum-packaged salami. Commercially produced salami was sliced and inoculated (4 ± 1.3 log CFU/cm2) with one of four types of inocula. All inocula consisted of the same 10-strain L. monocytogenes composite, c

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Jenkins, Carrie L., and Heather D. Bean. "Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition." Metabolites 10, no. 9 (2020): 347. http://dx.doi.org/10.3390/metabo10090347.

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In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of Staphylococcus aureus and Staphylococcus epidermidis. S. aureus (ATCC 12600) and S. epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media (brain heart infusion (BHI), lysogeny broth (LB), Mueller Hinton brot

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11

Karolenko, Caitlin E., Arjun Bhusal, Dhiraj Gautam, and Peter M. Muriana. "Selenite Cystine Agar for Enumeration of Inoculated Salmonella Serovars Recovered from Stressful Conditions during Antimicrobial Validation Studies." Microorganisms 8, no. 3 (2020): 338. http://dx.doi.org/10.3390/microorganisms8030338.

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Process validation studies often require the inoculation of select foodborne pathogens into targeted foods to determine the lethality of the process or antimicrobial ingredients, and quantitative recovery of surviving inoculum bacteria helps to make those assessments. Such processes introduce various stressors on the inoculated challenge microorganisms whereby traditional selective media are too harsh to enumerate the remaining viable and injured population quantitatively. Innate antibiotic resistance of challenge organisms has often been used to establish simple selective media (i.e., Tryptic

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12

Nolan, Richard A. "Physiological studies with the fungus Entomophaga aulicae during morphogenesis in three different media under fermentation conditions." Canadian Journal of Microbiology 39, no. 7 (1993): 701–8. http://dx.doi.org/10.1139/m93-100.

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The effects of three different media on amino acid uptake and production and glucose and oxygen utilization during protoplast growth and hyphal body production by the fungus Entomophaga aulicae under fermentation conditions were studied. The three media consisted of a basal medium plus either (i) 2.8% fetal calf serum, (ii) 0.8% tryptic soy broth plus 0.4% bovine serum albumin, or (iii) 0.8% tryptic soy broth plus 0.4% calcium caseinate. The protoplasts grew most rapidly (initial peaks on days 2 and 3) and hyphal bodies were detected first (day 3) in the media containing albumin and caseinate.

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BACON, R. T., J. N. SOFOS, P. A. KENDALL, K. E. BELK, and G. C. SMITH. "Comparative Analysis of Acid Resistance between Susceptible and Multi-Antimicrobial-Resistant Salmonella Strains Cultured under Stationary-Phase Acid Tolerance–Inducing and Noninducing Conditions." Journal of Food Protection 66, no. 5 (2003): 732–40. http://dx.doi.org/10.4315/0362-028x-66.5.732.

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This study compared acid resistance levels among five antimicrobial-susceptible strains of Salmonella and five strains that were simultaneously resistant to a minimum of six antimicrobial agents. The induction of a stationary-phase acid tolerance response (ATR) was attempted by both transient low-pH acid shock and acid adaptation. For acid shock induction, strains were grown for 18 h in minimal E medium containing 0.4% glucose (EG medium) and exposed to sublethal acid stress (pH 4.3) for 2 h, and subsequently, both shocked and nonshocked cultures were acid challenged (pH 3.0) for 4 h. Acid ada

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14

SOLOMON, ETHAN B., BRENDAN A. NIEMIRA, GERALD M. SAPERS, and BASSAM A. ANNOUS. "Biofilm Formation, Cellulose Production, and Curli Biosynthesis by Salmonella Originating from Produce, Animal, and Clinical Sources†." Journal of Food Protection 68, no. 5 (2005): 906–12. http://dx.doi.org/10.4315/0362-028x-68.5.906.

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The ability of 71 strains of Salmonella enterica originating from produce, meat, or clinical sources to form biofilms was investigated. A crystal violet binding assay demonstrated no significant differences in biofilm formation by isolates from any source when tested in any of the following three media: Luria-Bertani broth supplemented with 2% glucose, tryptic soy broth (TSB), or 1/20th-strength TSB. Incubation was overnight at 30°C under static conditions. Curli production and cellulose production were monitored by assessing morphotypes on Luria-Bertani agar without salt containing Congo red

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15

Rappert, Sugima, Kathrin Caroline Botsch, Stephanie Nagorny, Wittko Francke, and Rudolf Müller. "Degradation of 2,3-Diethyl-5-Methylpyrazine by a Newly Discovered Bacterium, Mycobacterium sp. Strain DM-11." Applied and Environmental Microbiology 72, no. 2 (2006): 1437–44. http://dx.doi.org/10.1128/aem.72.2.1437-1444.2006.

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ABSTRACT A bacterium was isolated from the waste gas treatment plant at a fishmeal processing company on the basis of its capacity to use 2,3-diethyl-5-methylpyrazine (DM) as a sole carbon and energy source. The strain, designated strain DM-11, grew optimally at 25°C and had a doubling time of 29.2 h. The strain did not grow on complex media like tryptic soy broth, Luria-Bertani broth, or nutrient broth or on simple carbon sources like glucose, acetate, oxoglutarate, succinate, or citrate. Only on Löwenstein-Jensen medium was growth observed. The 16S rRNA gene sequence of strain DM-11 showed

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GUERINI, MICHAEL N., TERRANCE M. ARTHUR, STEVEN D. SHACKELFORD, and MOHAMMAD KOOHMARAIE. "Evaluation of Escherichia coli O157:H7 Growth Media for Use in Test-and-Hold Procedures for Ground Beef Processing†." Journal of Food Protection 69, no. 5 (2006): 1007–11. http://dx.doi.org/10.4315/0362-028x-69.5.1007.

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Since the mid-1990s, the beef industry has used a process called test and hold, wherein beef trim and ground beef are tested to keep products contaminated with Escherichia coli O157:H7 out of commerce. Current O157:H7 detection methods rely on a threshold level of bacterial growth for detection, which is dependent on the growth medium used. Twelve media were examined for growth and doubling time: buffered peptone water (BPW), SOC (which contains tryptone, yeast extract, KCl, MgCl2, and glucose), buffered peptone water plus SOC (BPW-SOC), Bacto-NZYM, RapidChek E. coli O157:H7 medium, BioControl

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17

Lade, Harshad, Joon Hyun Park, Sung Hee Chung, et al. "Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media." Journal of Clinical Medicine 8, no. 11 (2019): 1853. http://dx.doi.org/10.3390/jcm8111853.

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Staphylococcus aureus (S. aureus) causes persistent biofilm-related infections. Biofilm formation by S. aureus is affected by the culture conditions and is associated with certain genotypic characteristics. Here, we show that glucose and sodium chloride (NaCl) supplementation of culture media, a common practice in studies of biofilms in vitro, influences both biofilm formation by 40 S. aureus clinical isolates (methicillin-resistant and methicillin-sensitive S. aureus) and causes variations in biofilm quantification. Methicillin-resistant strains formed more robust biofilms than methicillin-se

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18

FRATAMICO, PINA M. "Tolerance to Stress and Ability of Acid-Adapted and Non–Acid-Adapted Salmonella enterica Serovar Typhimurium DT104 To Invade and Survive in Mammalian Cells In Vitro†." Journal of Food Protection 66, no. 7 (2003): 1115–25. http://dx.doi.org/10.4315/0362-028x-66.7.1115.

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The ability of acid-adapted (AA) and non–acid-adapted (NA) Salmonella enterica serovar Typhimurium definitive type 104 (DT104) strains to invade and multiply in mammalian cells in vitro and to survive stress conditions was examined. DT104 and non-DT104 strains were grown in tryptic soy broth without glucose (NA) or in tryptic soy broth containing 1% glucose (AA) for 18 h at 37°C. The invasiveness of DT104 strains in J774A.1 macrophage and Int407 intestinal cell lines was not more extensive than that of non-DT104 strains. In most cases, AA bacteria were less invasive than NA bacteria in both ce

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Kim, Inhyup, Geeta Chhetri, Yoonseop So, Jiyoun Kim, and Taegun Seo. "Characteristics and Biological Activity of Exopolysaccharide Produced by Lysobacter sp. MMG2 Isolated from the Roots of Tagetes patula." Microorganisms 10, no. 7 (2022): 1257. http://dx.doi.org/10.3390/microorganisms10071257.

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In the present study, exopolysaccharide (EPS) produced by Lysobacter sp. MMG2 (lyEPS) was characterized and purified. The lyEPS-producing strain Lysobacter sp. MMG2 was isolated from the roots of Tagetes patula. When lyEPS was produced in tryptic soy broth with 1% glucose and the lyophilized powder was measured, the yield was found to be 0.67 g/L. The molecular weight (Mw) of lyEPS was 1.01 × 105 Da. Its monosaccharide composition includes 84.24% mannose, 9.73% glucose, 2.55% galactose, 2.77% arabinose, 0.32% xylose, and 0.03% rhamnose. Scanning electron microscopy (SEM) revealed that lyEPS ha

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Angel Villegas, Natalia, José Baronetti, Inés Albesa, et al. "Relevance of Biofilms in the Pathogenesis of Shiga-Toxin-ProducingEscherichia coliInfection." Scientific World Journal 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/607258.

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The present study was designed to determine the relationships among biofilm formation, cellular stress and release of Shiga toxin (Stx) by three different clinical Shiga toxin-producingEscherichia coli(STEC) strains. The biofilm formation was determined using crystal violet stain in tryptic soy broth or thioglycollate medium with the addition of sugars (glucose or mannose) or hydrogen peroxide. The reactive oxygen species (ROSs) were detected by the reduction of nitro blue tetrazolium and reactive nitrogen intermediates (RNI) determined by the Griess assay. In addition, the activities of two a

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Wu, Jian, Katheryn M. Goodrich, Joseph D. Eifert, et al. "Inhibiting foodborne pathogens Vibrio parahaemolyticus and Listeria monocytogenes using extracts from traditional medicine: Chinese gallnut, pomegranate peel, Baikal skullcap root and forsythia fruit." Open Agriculture 3, no. 1 (2018): 163–70. http://dx.doi.org/10.1515/opag-2018-0017.

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Abstract Foodborne illnesses have been a heavy burden in the United States and globally. Many medicinal herbs have been cultivated in the US and many of which contain antimicrobial compounds with the potential to be used for food preservation. Methanol/water extracts of pomegranate peel (“PP”, Punica Granatum L.), Chinese gallnut (“CG”, Galla chinensis), Forsythia fruit (“FF”, Forsythia suspensa) and Baikal skullcap root (“BS”, Scutellaria baicalensis) were tested for antimicrobial activity using the agar diffusion assay on tryptic soy agar (TSA) and microdilution assay in tryptic soy broth (T

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22

Fournier, Diane, Jalal Hawari, Annamaria Halasz, et al. "Aerobic Biodegradation of N-Nitrosodimethylamine by the Propanotroph Rhodococcus ruber ENV425." Applied and Environmental Microbiology 75, no. 15 (2009): 5088–93. http://dx.doi.org/10.1128/aem.00418-09.

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ABSTRACT The propanotroph Rhodococcus ruber ENV425 was observed to rapidly biodegrade N-nitrosodimethylamine (NDMA) after growth on propane, tryptic soy broth, or glucose. The key degradation intermediates were methylamine, nitric oxide, nitrite, nitrate, and formate. Small quantities of formaldehyde and dimethylamine were also detected. A denitrosation reaction, initiated by hydrogen atom abstraction from one of the two methyl groups, is hypothesized to result in the formation of n-methylformaldimine and nitric oxide, the former of which decomposes in water to methylamine and formaldehyde and

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23

Szultka-Młyńska, Małgorzata, Daria Janiszewska, Paweł Pomastowski, Michał Złoch, Wojciech Kupczyk, and Bogusław Buszewski. "Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach." Molecules 26, no. 16 (2021): 5007. http://dx.doi.org/10.3390/molecules26165007.

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The bacterial infection of post-operative wounds is a common health problem. Therefore, it is important to investigate fast and accurate methods of identifying bacteria in clinical samples. The aim of the study was to analyse the use of the MALDI-TOF MS technique to identify microorganism wounds that are difficult to heal. The most common bacteria are Escherichia coli, Staphylococcus spp., and Enterococcus spp. We also demonstrate the effect of culture conditions, such as the used growth medium (solid: Brain Heart Infusion Agar, Mueller Hilton Agar, Glucose Bromocresol Purple Agar, and Vancomy

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KIM, KWANG Y., and JOSEPH F. FRANK. "Effect of Growth Nutrients on Attachment of Listeria monocytogenes To Stainless Steel." Journal of Food Protection 57, no. 8 (1994): 720–24. http://dx.doi.org/10.4315/0362-028x-57.8.720.

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Listeria monocytogenes cells grown in chemically defined minimal medium (D10), tryptic soy broth (TSB), and modifications of these media were used to determine the effect of growth nutrients on attachment ability. Stainless steel surfaces were submerged in various cell suspensions at 21°C for 4 h, and the numbers of attached cells were compared. Cells grown in D10 showed approximately 50-fold higher attachment than those grown in TSB. Addition of components of D10 to TSB did not affect the attachment ability of cells grown in TSB. The only modifications of D10, which affected attachment abilit

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Pan, Youwen, Frederick Breidt, and Lisa Gorski. "Synergistic Effects of Sodium Chloride, Glucose, and Temperature on Biofilm Formation by Listeria monocytogenes Serotype 1/2a and 4b Strains." Applied and Environmental Microbiology 76, no. 5 (2010): 1433–41. http://dx.doi.org/10.1128/aem.02185-09.

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ABSTRACT Biofilm formation by Listeria monocytogenes is generally associated with its persistence in the food-processing environment. Serotype 1/2a strains make up more than 50% of the total isolates recovered from food and the environment, while serotype 4b strains are most often associated with major outbreaks of human listeriosis. Using a microplate assay with crystal violet staining, we examined biofilm formation by 18 strains of each serotype in tryptic soy broth with varying concentrations of glucose (from 0.25% to 10.0%, wt/vol), sodium chloride (from 0.5% to 7.0%, wt/vol) and ethanol (

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Caruso, Daniel J., Enzo A. Palombo, Simon E. Moulton, Peter J. Duggan, and Bita Zaferanloo. "Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia." Antibiotics 12, no. 9 (2023): 1459. http://dx.doi.org/10.3390/antibiotics12091459.

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The threat to public health resulting from the emergence of antimicrobial resistance (AMR) is ever rising. One of the major bacterial pathogens at the forefront of this problem is methicillin-resistant Staphylococcus aureus, or MRSA, for which there is a great need to find alternative treatments. One of the most promising alternatives is endophytic fungi, which were shown to produce a vast array of bioactive compounds, including many novel antibacterial compounds. In this study, two endophytic Alternaria sp., EL 24 and EL 35, were identified from the leaves of Eremophila longifolia. Ethyl acet

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Wu, Wen Tung, and Ming Der Jean. "Evaluation of Light Irradiation on Decolorization of Azo Dyes by Tsukamurella sp. J8025." Applied Mechanics and Materials 145 (December 2011): 304–8. http://dx.doi.org/10.4028/www.scientific.net/amm.145.304.

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In the previous study, the dye decolorization was investigated byTsukamurellasp. J8025 under the static condition at 30°C. The object of this study was to evaluate the influence of light irradiation with 15W low-pressure mercury lamp on dye decolorization. Three kinds of common culture medium Luria-Bertani (LB), Tryptic Soy Broth (TSB), and Yeast extract-Malt Extract (YEME) were used in this study. Strain J8025 was cultivated in different media added with methyl orange, and the rate of color removal was determined by measuring the absorbance at specific wavelengths. The experiments proved the

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Steixner, Stephan Josef Maria, Christopher Spiegel, Dietmar Dammerer, Alexander Wurm, Michael Nogler, and Débora Cristina Coraça-Huber. "Influence of Nutrient Media Compared to Human Synovial Fluid on the Antibiotic Susceptibility and Biofilm Gene Expression of Coagulase-Negative Staphylococci In Vitro." Antibiotics 10, no. 7 (2021): 790. http://dx.doi.org/10.3390/antibiotics10070790.

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Bacterial antibiotic resistance and biofilm formation are mechanisms usually involved in the pathogeny of implant-related infections. Worldwide, antibiotic susceptibility tests are usually carried out using nutrient-rich media. Clinical routine laboratories and even research centers use for example EUCAST or CLSI for guidelines. In this study, we investigated the effect of different nutrient media on the antibiotic susceptibility and icaADBC gene expression of bacteria in biofilm. As media, Müller-Hinton Bouillon (MHB), Tryptic Soy Broth (TSB) and human synovial fluid (SF) diluted 1:4 in phosp

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ANNOUS, BASSAM A., and MICHAEL F. KOZEMPEL. "Influence of Growth Medium on Thermal Resistance of Pediococcus sp. NRRL B-2354 (Formerly Micrococcus freudenreichii) in Liquid Foods†." Journal of Food Protection 61, no. 5 (1998): 578–81. http://dx.doi.org/10.4315/0362-028x-61.5.578.

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Pediococcus sp. is a nonpathogenic heat-resistant spoilage organism that has been used as a test organism in milk pasteurization studies. These characteristics make this bacterium an attractive test organism to study the mode of bacterial thermal inactivation in a food pilot plant. We report here the effect of growth medium on the thermal D value of this organism in skim milk, whole liquid egg, 10% glucose solution, pineapple juice, apple juice, tomato juice, and water at 60°C. Thermal inactivation was done in a submerged coil; D values were calculated from the linear portion of the survival c

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RYU, JEE-HOON, YUN DENG, and LARRY R. BEUCHAT. "Behavior of Acid-Adapted and Unadapted Escherichia coli O157:H7 When Exposed to Reduced pH Achieved with Various Organic Acids." Journal of Food Protection 62, no. 5 (1999): 451–55. http://dx.doi.org/10.4315/0362-028x-62.5.451.

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A study was done to determine if various organic acids differ in their inhibitory or lethal activity against acid-adapted and unadapted Escherichia coli O157:H7 cells. E. coli O157:H7 strain E0139, isolated from venison jerky, was grown in tryptic soy broth (TSB) and in TSB supplemented with 1% glucose (TSBG) for 18 h at 37°C, then plated on tryptic soy agar (TSA) acidified with malic, citric, lactic, or acetic acid at pH 5.4, 5.1, 4.8, 4.5, 4.2, and 3.9. Regardless of whether cells were grown in TSB or TSBG, visible colonies were not formed when plated on TSA acidified with acetic, lactic, ma

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Bacon, R. T., J. R. Ransom, J. N. Sofos, P. A. Kendall, K. E. Belk, and G. C. Smith. "Thermal Inactivation of Susceptible and Multiantimicrobial-Resistant Salmonella Strains Grown in the Absence or Presence of Glucose." Applied and Environmental Microbiology 69, no. 7 (2003): 4123–28. http://dx.doi.org/10.1128/aem.69.7.4123-4128.2003.

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ABSTRACT The heat resistance of susceptible and multiantimicrobial-resistant Salmonella strains grown to stationary phase in glucose-free tryptic soy broth supplemented with 0.6% yeast extract (TSBYE−G; nonadapted), in regular (0.25% glucose) TSBYE, or in TSBYE−G with 1.00% added glucose (TSBYE+G; acid adapted) was determined at 55, 57, 59, and 61°C. Cultures were heated in sterile 0.1% buffered peptone water (50 μl) in heat-sealed capillary tubes immersed in a thermostatically controlled circulating-water bath. Decimal reduction times (D values) were calculated from survival curves having r 2

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Alves de Lima e Silva, Agostinho, Alice Slotfeldt Viana, Priscila Martins Silva, et al. "Diclofenac May Induce PIA-Independent Biofilm Formation in Staphylococcus aureus Strains." International Journal of Microbiology 2021 (January 27, 2021): 1–11. http://dx.doi.org/10.1155/2021/8823775.

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Staphylococcus aureus is a pathogen commonly resistant to antibiotics. Biofilm formation is one of the important factors related to its virulence. Non-antibiotics drugs, such as nonsteroidal anti-inflammatory agents (NSAIDs), have been studied as an alternative for treating infections by multiresistant pathogens and biofilm-associated infections. In this study, the effects of NSAID sodium diclofenac on growth inhibition and biofilm formation of S. aureus were evaluated. The minimum inhibitory concentration (MIC) of diclofenac for fifty isolates ranged from 200 to 400 μg/mL. Diclofenac sub-MICs

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Pazlarová, J., S. Purkrtová, J. Babulíková, and K. Demnerová. "Effects of ampicillin and vancomycin on Staphylococcus aureus biofilms." Czech Journal of Food Sciences 32, No. 2 (2014): 137–44. http://dx.doi.org/10.17221/156/2013-cjfs.

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The collection of 23 coagulase-positive Staphylococcus aureus strains isolated mainly from food in the Czech Republic were tested on the ability to form biofilms in the presence of ampicillin and vancomycin. The antimicrobial sensitivity (16&nbsp;antibiotics) was determined in all strains by the standard disc diffusion method on Mueller-Hinton agar plates (NCCLS). The resistance to ampicillin was found in 16 strains (69.5%), all strains being susceptible to vancomycin. The formation of biofilm was conducted in 96-well, polystyrene microtiter plates COSTAR 3797 in tryptic soy broth (TSB) wi

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Annous, Bassam A., Michael F. Kozempel, and Michael J. Kurantz. "Changes in Membrane Fatty Acid Composition ofPediococcus sp. Strain NRRL B-2354 in Response to Growth Conditions and Its Effect on Thermal Resistance." Applied and Environmental Microbiology 65, no. 7 (1999): 2857–62. http://dx.doi.org/10.1128/aem.65.7.2857-2862.1999.

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ABSTRACT Membrane fatty acid composition and thermal resistance (D value) of Pediococcus sp. were determined for mid-exponential-phase (ME) and stationary-phase (ST) cells grown in tryptic soy broth (TSB) and tryptone-glucose-yeast extract (TGY) at 28 and 37°C. As the cells entered the stationary phase of growth, the unsaturated fatty acid, C18:1 n11c, produced during the exponential phase of growth was converted to its cyclic form, C19:0 Δ9c. This shift in membrane fatty acid composition was accompanied by an increase in the D values of this bacterium. Data from this study suggest that the me

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Iwabuchi, Yusuke, Hiroko Yoshida, Shuichiro Kamei, Toshiki Uematsu, Masanori Saito, and Hidenobu Senpuku. "Formation of Mono-Organismal and Mixed Staphylococcus aureus and Streptococcus mutans Biofilms in the Presence of NaCl." Microorganisms 13, no. 5 (2025): 1118. https://doi.org/10.3390/microorganisms13051118.

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Staphylococcus aureus, an opportunistic bacterium found in the oral cavity, has been reported as a causative agent of infective endocarditis and pneumonia. Salt is an essential mineral for cell maintenance in the human body. This study was conducted to clarify how salt affects the formation of biofilms by S. aureus and Streptococcus mutans, pathogens implicated in dental caries. Bacteria were cultivated with various concentrations of NaCl on a 96-well microtiter plate in tryptic soy broth with 0.25% sucrose or 0.25% glucose (TSBs and TSBg, respectively) for 16 h. The effects of glucosyltransfe

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BAYLES, DARRELL O. "Changes in Heat Resistance Resulting from pH and Nutritional Shifts of Acid-Adapted and Non–Acid-Adapted Listeria monocytogenes Scott A†." Journal of Food Protection 67, no. 2 (2004): 316–21. http://dx.doi.org/10.4315/0362-028x-67.2.316.

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Stationary-phase Listeria monocytogenes cells that were either pH dependent acid adapted or not acid adapted were heat challenged at 60°C in a two-level full factorial design for three variables. The three variables and the levels consisted of tryptic soy broth (TSB) and sterile cell-free culture supernatant (sterile TSB), the presence and absence of 1% added glucose, and pH 4.8 and pH 7. Non–acid-adapted cells were most heat resistant when challenged in TSB (mean decimal reduction times at 60°C: D60 = 1.16 min). In the absence of added glucose, non–acid-adapted cells had similar D60-values fo

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Brandileone, Maria Cristina de Cunto, Rosemeire Cobo Zanella, Vera Simonsen Dias Vieira, Claudio Tavares Sacciii, Lucimar Gonçalves Milagres, and Carl Eduard Frasch. "Induction of iron regulated proteins during normal growth of Neisseria meningitidis in a chemically defined medium." Revista do Instituto de Medicina Tropical de São Paulo 36, no. 4 (1994): 301–10. http://dx.doi.org/10.1590/s0036-46651994000400002.

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The expression of iron regulated proteins (IRPs) in vitro has been obtained in the past by adding iron chelators to the culture after bacterial growth, in the presence of an organic iron source. We have investigated aspects concerning full expression of the meningococcal IRPs during normal growth, in defined conditions using Catlin medium, Mueller Hinton and Tryptic Soy Broth (TSB). The expression of IRPs varied between different strains with respect to Ethylenediamine Di-ortho-Hidroxy-phenyl-acetic acid (EDDA) concentrations, and according to culture medium, and also between different lots of

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Kim, Daseul, and Ki-Young Kim. "Pectolinarin Inhibits the Bacterial Biofilm Formation and Thereby Reduces Bacterial Pathogenicity." Antibiotics 11, no. 5 (2022): 598. http://dx.doi.org/10.3390/antibiotics11050598.

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Bacterial biofilms are a growing problem as it is a major cause of nosocomial infection from urinary catheters to chronic tissue infections and provide resistance to a variety of antibiotics and the host’s immune system. The effect of pectolinarin on the biofilm formation in Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Streptococcus mutans, Streptococcus sobrinus, Staphylococcus aureus, Pseudomonas aeruginosa, Cutibacterium acnes, and Porphyromonas gingivalis was studied in TSBg (tryptic soy broth supplemented with 1% glucose). Pectolinarin inhibited biofilm formation of E. f

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Hu, Xueyu, Xiaoxu Wang, Honglin Ren, et al. "Preliminary Study of the Characterization of the Viable but Noncultivable State of Yersinia enterocolitica Induced by Chloride and UV Irradiation." Microorganisms 12, no. 9 (2024): 1778. http://dx.doi.org/10.3390/microorganisms12091778.

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The viable but non-culturable (VBNC) state is a survival strategy for many foodborne pathogens under adverse conditions. Yersinia enterocolitica (Y. enterocolitica) as a kind of primary foodborne pathogen, and it is crucial to investigate its survival strategies and potential risks in the food chain. In this study, the effectiveness of ultraviolet (UV) irradiation and chlorine treatment in disinfecting the foodborne pathogen Y. enterocolitica was investigated. The results indicated that both UV irradiation and chlorine treatment can induce the VBNC state in Y. enterocolitica. The bacteria comp

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SKANDAMIS, PANAGIOTIS N., JARRET D. STOPFORTH, YOHAN YOON, PATRICIA A. KENDALL, and JOHN N. SOFOS. "Heat and Acid Tolerance Responses of Listeria monocytogenes as Affected by Sequential Exposure to Hurdles during Growth." Journal of Food Protection 72, no. 7 (2009): 1412–18. http://dx.doi.org/10.4315/0362-028x-72.7.1412.

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This study aimed to evaluate the effects of the level and sequence of hurdles, applied during growth, on the subsequent heat and acid tolerances of a 10-strain composite of Listeria monocytogenes. Individual strains were grown in glucose-free tryptic soy broth with 0.6% yeast extract (TSBYE–G). Then cultures were mixed and inoculated in fresh TSBYE–G (0.5% NaCl, pH 7.42; control), TSBYE–G that was supplemented with 3% NaCl (3.5% NaCl in total), or TSBYE–G with pH adjusted to 6.01 or 5.04 with lactic acid and incubated at 30°C for 24 h. Furthermore, the culture composite was exposed to the foll

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Fujikawa, Hiroshi, and Ryo Akimoto. "New Blue Pigment Produced byPantoea agglomeransand Its Production Characteristics at Various Temperatures." Applied and Environmental Microbiology 77, no. 1 (2010): 172–78. http://dx.doi.org/10.1128/aem.00264-10.

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ABSTRACTA bacterium capable of producing a deep blue pigment was isolated from the environment and identified asPantoea agglomerans. The pigment production characteristics of the bacterium under various conditions were studied. The optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at pH 6.4. Bacterial cells grew on the agar plate during the incubation, while the pigment spread into the agar plate, meaning that it is water soluble. Pigment production was

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Amandita, F. Y., Efadeswarni, A. Z. N. Ikhwani, et al. "The growth of mercury-resistant bacterial consortium at different levels of pH, temperature, and C:N ratio." IOP Conference Series: Earth and Environmental Science 1201, no. 1 (2023): 012068. http://dx.doi.org/10.1088/1755-1315/1201/1/012068.

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Abstract The study was conducted to investigate the growth of mercury-resistant bacterial consortium under the influence of different pHs, temperatures, and C:N ratios, as a part of mercury bioremediation research. The consortium consisted of five bacterial isolates selected due to their resistance to HgCl2 up to 100 ppm and was proven to effectively reduce mercury from the culture media. Consortium suspension was inoculated into two sets of media: 1) Tryptic soy broth (TSB) with pHs of 4.8, 6.1, 8.5, and 9.7; 2) Mineral salt with the addition of glucose and peptone to create C:N ratios of 20:

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Purkrtová, S., J. Babulíková, R. Karpíšková, K. Demnerová, and J. Pazlarová. "Antimicrobial factors effects on biofilm formation in Staphylococcus aureus." Czech Journal of Food Sciences 29, Special Issue (2012): S1—S10. http://dx.doi.org/10.17221/319/2011-cjfs.

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We determined the disinfectant effects of benzalkonium chloride (BC) and Savo (SV), a chlorine compound, on the biofilm and planktonic cells in 23 strains S. aureus mainly food isolates. The biofilm formation was performed in a model system using microtiter polystyrene plates COSTAR 3797 in trypton-soy broth with 1% glucose at 30&deg;C. Benzalkonium chloride (BC) at 125 mg/l, applied directly on 24 h old biofilm, was able to remove the biofilm matrix in 21 strains, and to stop the reproduction of the biofilm cells in 23 strains. BC at the concentration of 125 mg/l was lethal to planktonic

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Tu, Le Thi Anh, та Le Ba Le. "Optimization of β-D-galactosidase rapid enzyme assay using Escherichia coli ATCC 8739". Vietnam Journal of Biotechnology 14, № 2 (2016): 361–67. http://dx.doi.org/10.15625/1811-4989/14/2/9362.

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The bacterial enzyme, β-D-galactosidase, catalyzes the breakdown of the complex sugar lactose into its components - galactose and glucose- simple sugars. The glycolysis of lactose by β-D-galactosidase is a point of attack for studies of the biochemical problem in disaccharide utilization and the genetic basis of enzyme constitution β-D galactosidase. The aim of the present study was to focus on optimization of a rapid enumeration method based on the enzymatic hydrolysis of 4-methylumbelliferyl-β-D-galactoside (MUGal) for toxicity test using Escherichia coli (E. coli) ATCC 8739 as a model. This

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Hingston, Patricia A., Marta J. Piercey, and Lisbeth Truelstrup Hansen. "Genes Associated with Desiccation and Osmotic Stress in Listeria monocytogenes as Revealed by Insertional Mutagenesis." Applied and Environmental Microbiology 81, no. 16 (2015): 5350–62. http://dx.doi.org/10.1128/aem.01134-15.

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ABSTRACTListeria monocytogenesis a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700L. monocytogenesmutants was screened, using a microplate assay, for strains displaying increased or decreased desiccation survival (43% relative humidity, 15°C) in tryptic soy broth (TSB). The desiccation phenotypes of selected mutants were subsequently assessed on food-grade stainless steel (SS) coupons in TSB plus 1% glucos

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Fernández-Barat, Laia, Ruben López-Aladid, Nil Vázquez, et al. "Bacterial Adaptive Memory in Methicillin-Resistant Staphylococcus aureus from Endotracheal Tubes." Pathogens 13, no. 2 (2024): 144. http://dx.doi.org/10.3390/pathogens13020144.

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Objectives: To evaluate the expression dynamics of biofilm genes in methicillin-resistant Staphylococcus aureus (MRSA) retrieved from endotracheal tubes (ETT) and to determine how gene regulation is attenuated in vitro where host–environmental factors are no longer present. Methods: Biofilm was grown (24 h) in tryptic broth soy plus 0.25% glucose for a clinical MRSA isolate in planktonic state and after sessile growth named ETT-MRSA (S2, S3, S4, S5, S6, S7). Gene expression of five biofilm-related genes (icaC, clfB, ebps, fnbB, and RNA III) was assessed consecutively from day 1 to day 4 after

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47

Nguyễn, Khắc Tiệp, та Thành Lập Vũ. "Nghiên cứu tạo và đánh giá biofilm của <i>Staphylococus aureus</i> trên một số bề mặt vật liệu". Tạp chí Nghiên cứu Dược và Thông tin Thuốc 21 (23 січня 2025): 20–26. https://doi.org/10.59882/1859-364x/261.

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Nghiên cứu được thiết kế để tạo và đánh giá biofilm của Staphylococcus aureus trên 4 bề mặt inox, nhôm, kính, nhựa polystyren, với mong muốn ứng dụng để nghiên cứu các hoạt chất kháng biofilm của vi sinh vật trên các bề mặt này. Nghiên cứu sử dụng tụ cầu vàng S. aureus ATCC 33591, với nồng độ ban đầu 107 CFU/ml, được nuôi cấy trong môi trường Tryptic Soy Broth có bổ sung glucose 1% và natri clorid 2%, cho khả năng tạo biofilm hoàn chỉnh sau 24 giờ ủ ở 37°C, trên cả 4 bề mặt. Biofilm tạo thành trên các bề mặt đều ổn định ít nhất 48 giờ, cả về số lượng vi sinh vật sống trong biofilm và tổng sinh

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Babic, Isabelle, and Alley E. Watada. "Inhibitory Effect of Fresh-cut Spinach on Listeria monocytogenes." HortScience 30, no. 4 (1995): 890C—890. http://dx.doi.org/10.21273/hortsci.30.4.890c.

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Studies. were undertaken to investigate the cause of the bacteriostatic activity of spinach leaves against Listeria monocytogenes. Listeria monocytogenes was inoculated at the concentration 104 cells/ml in raw, autoclaved and vacuum filtration sterilized spinach macerates as well as in tryptic soy broth used as control. The concentrations after 24 h at 28C were 105, 5 × l06 and 105 cells/ml in respective spinach macerates and 4 × l08 cells/ml in tryptic soy broth. The anti-Listeria activity was still present in spinach macerates sterilized by vacuum filtration but strongly reduced in autoclave

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Henry-Stanley, Michelle J., Donavon J. Hess, and Carol L. Wells. "Aminoglycoside inhibition of Staphylococcus aureus biofilm formation is nutrient dependent." Journal of Medical Microbiology 63, no. 6 (2014): 861–69. http://dx.doi.org/10.1099/jmm.0.068130-0.

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Biofilms represent microbial communities, encased in a self-produced matrix or extracellular polymeric substance. Microbial biofilms are likely responsible for a large proportion of clinically significant infections and the multicellular nature of biofilm existence has been repeatedly associated with antibiotic resistance. Classical in vitro antibiotic-susceptibility testing utilizes artificial growth media and planktonic microbes, but this method may not account for the variability inherent in environments subject to biofilm growth in vivo. Experiments were designed to test the hypothesis tha

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Chiou, R. Y. Y., R. D. Phillips, P. Zhao, M. P. Doyle, and L. R. Beuchat. "Ethanol-Mediated Variations in Cellular Fatty Acid Composition and Protein Profiles of Two Genotypically Different Strains of Escherichia coli O157:H7." Applied and Environmental Microbiology 70, no. 4 (2004): 2204–10. http://dx.doi.org/10.1128/aem.70.4.2204-2210.2004.

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ABSTRACT Two strains of Escherichia coli O157:H7 were grown in tryptic soy broth (TSB, pH 7.1) supplemented with 0, 2.5, 5.0, 7.5, and 10% ethanol at 30°C for up to 54 h. Growth rates in TSB supplemented with 0, 2.5, and 5.0% ethanol decreased with an increase in ethanol concentration. Growth was not observed in TSB supplemented with 7.5 or 10% ethanol. The pH of TSB containing 5.0% ethanol decreased to 5.8 within 12 h and then increased to 7.0 at 54 h. The ethanol content in TSB supplemented with 2.5 or 5.0% ethanol did not change substantially during the first 36 h of incubation but decrease

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