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Journal articles on the topic 'Tuberculose - Microbiologia'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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1

Zamarioli, Liliana Aparecida, Andréa Gobetti Vieira Coelho, Clemira Martins Pereira, Lucilaine Ferrazoli, and Ricardo Helbert Bammann. "Identificação laboratorial de micobactérias em amostras respiratórias de pacientes HIV-positivos com suspeita de tuberculose." Revista da Sociedade Brasileira de Medicina Tropical 42, no. 3 (2009): 290–97. http://dx.doi.org/10.1590/s0037-86822009000300010.

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Foram analisados retrospectivamente os registros (2000 a 2004) do Laboratório de Microbiologia do Instituto Adolfo Lutz de Santos, SP referentes a pacientes infectados pelo virus da imunodeficiência humana com suspeita de tuberculose pulmonar. Foram encaminhadas 1.321 amostras com finalidade de diagnóstico, correspondendo a 880 casos suspeitos de tuberculose em 693 pacientes. Cento e trinta e quatro baciloscopias foram positivas e em 188 culturas houve crescimento de micobactérias, correspondendo a 161 casos confirmados. Houve identificação de Mycobacterium tuberculosis em 126 (78,3%) e micoba

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2

Galvão, Maria Aparecida, João Cesar da Silva, and Mônica Cristina Teixeira. "Eficácia da descontaminação de resíduos biológicos infectantes de laboratórios de microbiologia após tratamento térmico por autoclavação." Engenharia Sanitaria e Ambiental 18, no. 4 (2013): 323–31. http://dx.doi.org/10.1590/s1413-41522013000400004.

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A pesquisa foi realizada no Laboratório Central de Saúde Pública de Minas Gerais com o objetivo de validar o processo de descontaminação de resíduos infectantes do subgrupo A1 e identificar possíveis falhas no procedimento preliminar à sua disposição final. Foram avaliados tanto a descontaminação dos resíduos totalmente descartáveis acondicionados em sacos plásticos termorresistentes quanto o processo de descontaminação dos resíduos reutilizáveis provenientes do Laboratório de Tuberculose, acondicionados em caixas metálicas. Enquanto os resultados obtidos no primeiro estudo indicaram uma defic

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Hammi, Sanae, Naima Zimed, Khalid Bouti, and Jamal Eddine Bourkadi. "Paradoxical reactions during Antituberculosis therapy - A single-center prospective analysis." International Journal of Medicine and Surgery 2, no. 2 (2015): 32–35. http://dx.doi.org/10.15342/ijms.v2i2.75.

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[1] Hawkey CR, Yap T, Pereira J, Moore DA, Davidson RN, Pasvol G, et al. Characterization and management of paradoxical upgrading reactions in HIV-uninfected patients with lymph node tuberculosis. Clinical infectious diseases. 2005;40(9):1368-71. [2] Breton G. Syndrome inflammatoire de reconstitution immune (IRIS) associé à la tuberculose. Journal des Anti-infectieux. 2012;14(4):180-5. [3] Cheng V, Ho P, Lee R, Chan K, Woo P, Lau S, et al. Clinical spectrum of paradoxical deterioration during antituberculosis therapy in non-HIV-infected patients. European Journal of Clinical Microbiology and I

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4

Carvalho, Márcio Pedrote, Luciana Sant`Ana Souza, Jair Antonio Carvalho, and Bruno Medrado Araújo. "Fatores de risco e soroprevalência da Brucelose em assentamento rural no Município de Aragominas - TO, Brasil." Cadernos UniFOA 8, no. 22 (2013): 81. http://dx.doi.org/10.47385/cadunifoa.v8i22.30.

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Foi realizado um estudo descritivo transversal ou de prevalência, com o objetivo de identificar a positividade de reações sorológicas para brucelose em bovinos, em projeto de assentamento do Município de Aragominas, Estado do Tocantins, Brasil, no ano de 2008. Neste estudo a amostra foi constituída de 198 bovinos submetidos a provas sorológicas para brucelose. Conforme preconizado pelo Programa de Controle e Erradicação da Brucelose e Tuberculose - PNCEBT, as provas realizadas foram, de triagem a prova do Antígeno Acidificado Tamponado (AAT), realizada no laboratório de Fundação de Medicina Tr

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Opanasenko, N. S., I. V. Liskina, V. I. Lysenko, et al. "Morphological studies of the surgical material to determine the formphase and the degree of activity of the tuberculosis process when using minimally invasive surgical treatment techniques." Tuberculosis, Lung Diseases, HIV Infection, no. 1 (March 23, 2021): 25–33. http://dx.doi.org/10.30978/tb2021-1-25.

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Objective — to analyze the morphological studies of the operating material with the determination of the form-phase and the level of activity of the tuberculous process using minimally invasive methods of surgical treatment. Materials and methods. A retrospective analysis of data for a five-year period was conducted in our clinic. A total of 165 cases of pulmonary or pleural tuberculosis were treated. Patients were of different age groups. The men were 88 (53.3 %), women — 77 (46.6 %). All patients underwent morphological examination of the surgical material. Morphological determination

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Dariansyah, Ahmad Data, Wihasto Suryaningtyas, and Muhammad Arifin Parenrengi. "Tuberculoma mimicking postoperative VP shunt seeding of craniopharyngioma: A rare case report." Surgical Neurology International 12 (September 6, 2021): 450. http://dx.doi.org/10.25259/sni_606_2021.

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Background: Tuberculosis (TB) is still a big problem in developing and TB endemic countries such as Indonesia. The most common manifestations of TB in the central nervous system are tuberculous meningitis and tuberculoma. In developing and TB endemic countries, tuberculomas account for 33% of intracranial space-occupying lesions. Isolated tuberculoma without systemic TB is rarely seen. On physical and radiological examination, tuberculoma often gives an atypical appearance. From imaging, tuberculoma often mimics another intracranial tumor. Oftentimes the accurate diagnosis can only be made aft

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7

Sulistyowati, Titiek, Deby Kusumaningrum, Eko Budi Koendhori, and Ni Made Mertaniasih. "Tuberculous Meningitis: The Microbiological Laboratory Diagnosis and Its Drug Sensitivity Patterns." Jurnal Respirasi 3, no. 2 (2019): 35. http://dx.doi.org/10.20473/jr.v3-i.2.2017.35-40.

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Background: Tuberculosis continues one of the major challenges to global health. Mycobacterium tuberculosis complex can affect any organ other than the lung parenchyma, include central nervous system. The mortality rate of tuberculous meningitis (TBM) are high worldwide with up to half of survivors suffering irreversible sequelae. Diagnosis of TBM is difficult due to paucibacillary, various clinical manifestation, and invasive procedure to appropriate specimens. Objective: The objectiveis to study the positivity rate of microbiological laboratory diagnosis and its drug sensitivity patterns of

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Ali, Hosameldeen Mostafa, and Hoda Mohamed Abdelaziz Shoshan. "Multifocal primary musculoskeletal tuberculosis in nonimmunocompromised patient from nonendemic area." BJR|case reports 6, no. 2 (2020): 20190077. http://dx.doi.org/10.1259/bjrcr.20190077.

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Iliopsoas primary tuberculous abscess is a rare clinical and imaging entity. Most reported imaging literature cases are secondary to tuberculous spondylodiscitis. Iliopsoas tuberculous inflammation and abscess constitutes a diagnostic challenge owing to its insidious onset and subtle non-specific symptoms. Here, in a case of right iliopsoas and thigh primary tuberculosis abscess complicated with right iliac bone osteomyelitis extended to the right hip joint. The conventional radiography, thigh and pelvic ultrasonography, MRI and CT examinations showed the whole right iliopsoas and thigh absces

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Jesic, Snezana, Svetlana Stosic, Branislava Milenkovic, et al. "Middle ear tuberculosis: Diagnostic criteria." Srpski arhiv za celokupno lekarstvo 137, no. 7-8 (2009): 346–50. http://dx.doi.org/10.2298/sarh0908346j.

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Introduction. Tuberculous otitis is a diagnostic problem due to the difficulty to obtain microbiological, histomorphological and cytological confirmation of the disease. Objective. Our objective was to compare clinical and radiological characteristic and development of otogenic complications in patients with tuberculous otitis and otitis with cholesteatoma as the most destructive form of chronic nonspecific otitis in the purpose of establishing the diagnostic criteria for tuberculous otitis. Methods. Medical records of 12 patients with tuberculous otitis and 163 patients with cholesteatoma tre

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M Pollock, Katrina, and Ajit Lalvani. "Tuberculosis of the central nervous system: Recognition, diagnosis and treatment in a low-prevalence country." Acute Medicine Journal 7, no. 3 (2008): 113–21. http://dx.doi.org/10.52964/amja.0212.

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Abstract Tuberculosis (TB) is one of the greatest threats to global public health, with 9.2 million new cases in 20061 and has become increasingly common in the UK. Central nervous system (CNS) infection with Mycobacterium tuberculosis (MTB) is relatively rare but is associated with a serious risk of neurological morbidity or death. Delays in diagnosis worsen prognosis and even with anti-tuberculous therapy up to 30% of tuberculous meningitis (TBM) patients may die.2 TBM and tuberculomas can mimic other CNS pathologies. Careful analysis of the clinical features, CSF examination and pragmatic u

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Stogova, Natalya. "DIAGNOSIS OF TUBERCULOUS PLEURISY IN PATIENTS WITH ONCOLOGICAL DISEASES." Problems in oncology 65, no. 3 (2019): 399–403. http://dx.doi.org/10.37469/0507-3758-2019-65-3-399-403.

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The analysis of the effectiveness of a set of measures for the differential diagnosis of tuberculosis and tumor etiology of pleural effusion in patients with cancer. It was established that out of 1934 patients with pleural effusion, having malignant tumors of different localization, pleural etiology was diagnosed in 23 (1.2%). Insufficient efficiency of etiological diagnostics of pleural effusion in the conditions of institutions of the General medical network is revealed. The average period from the onset of clinical manifestations of tuberculous pleurisy to admission of patients to the hosp

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Ergeshov, Atadzhan E., Larisa N. Chernousova, and Sofya N. Andreevskaya. "New technologies for the diagnosis of drug-resistant tuberculosis." Annals of the Russian academy of medical sciences 74, no. 6 (2020): 413–22. http://dx.doi.org/10.15690/vramn1163.

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Widespread drug-resistant tuberculosis is a global challenge that has a potential to have a negative impact on the global TB situation. Rapid TB diagnosis and early initiation of effective treatment based on the individualized chemotherapy regimens underpin efforts in prevention of further spread of tuberculosis. Microbiological methods of TB diagnosis that provide evidence of the TB process etiology and identify drug susceptibility of the pathogen play a very important role. The review covers the current and emerging methods of the microbiological TB diagnosis including conventional microbiol

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Kameswaran, M., K. Natarajan, M. Parthiban, P. V. Krishnan, and S. Raghunandhan. "Tuberculous otitis media: a resurgence?" Journal of Laryngology & Otology 131, no. 9 (2017): 785–92. http://dx.doi.org/10.1017/s0022215117001281.

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AbstractObjective:Tuberculosis is a global health problem that is especially prevalent in developing countries such as India. Recently, atypical presentation has become more common and a high index of suspicion is essential. This study analysed the various presenting symptoms and signs of tuberculous otitis media and the role of diagnostic tests, with the aim of formulating criteria for the diagnosis.Methods:A total of 502 patients underwent tympanomastoidectomy over a two-year period. Microbiological and histopathological examinations and polymerase chain reaction analysis of tissue taken dur

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Krishna, Nallamothu Murali, Vietla Sreeramulu, Sai Krishna Gurrala, and T. Jaya Chandra. "Aetiopathological evaluation of pleural effusions." International Journal of Advances in Medicine 7, no. 1 (2019): 90. http://dx.doi.org/10.18203/2349-3933.ijam20195651.

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Background: Pleural Effusion (PE) is a sign of disease and not a diagnosis itself. Hence an attempt was made to find out the etiological diagnosis of pleural effusion in cases.Methods: Single center, observational, cross sectional hospital-based study. Samples were collected by systematic random sampling method; study was conducted in GSL Medical College. Patients admitted with pleural effusion in medical wards and ICU were taken. The patients >14 years age, both genders were included. All patients were interviewed to obtain detailed history and examined thoroughly as per pre-determined pro

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Cvetkovikj, Iskra, Slavcho Mrenoshki, Kiril Krstevski, et al. "Bovine Tuberculosis in the Republic of Macedonia: Postmortem, Microbiological and Molecular Study in Slaughtered Reactor Cattle." Macedonian Veterinary Review 40, no. 1 (2017): 43–52. http://dx.doi.org/10.1515/macvetrev-2016-0097.

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Abstract Bovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonotic potential. The main purpose of the study was to determine the presence and distribution of the tuberculous lesions in reactor cattle, and to isolate and identify the causative agents of bovine tuberculosis in the Republic of Macedonia. Lymph nodes and affected organs from 188 reactor catt

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SUMBUL, BILGE, and MEHMET ZIYA DOYMAZ. "A Current Microbiological Picture of Mycobacterium Isolates from Istanbul, Turkey." Polish Journal of Microbiology 69, no. 2 (2020): 185–91. http://dx.doi.org/10.33073/pjm-2020-021.

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Despite advances in diagnosis and treatment, tuberculosis (TB) continues to be one of the essential health problems throughout the world. Turkey is considered to be endemic for TB. In this study, we analyzed the distribution of Mycobacterium species, compare the diagnostic methods, and susceptibilities to anti-tuberculosis drugs of TB isolates. The aim was to document the current status and to provide a frame of reference for future studies. In this study, 278 Mycobacterium species isolated from 7,480 patients between September 2015 and June 2019 were included. Löwenstein-Jensen medium (LJ) an

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Skodric-Trifunovic, Vesna. "New insights into transmission of tuberculosis." Medical review 57, no. 11-12 (2004): 561–65. http://dx.doi.org/10.2298/mpns0412561s.

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Introduction Considering the fact that tuberculosis is a global problem, that one third of the world population is infected with tuberculosis and that 8-10 million of newly recognized cases are detected per year, the need for acquiring new knowledge on pathogenesis and transmission of tuberculosis becomes obvious. Application of molecular biology methods in clinico-epidemiology practice has identified transmission chain of tuberculous bacilli in numerous intrahospital outbreaks of tuberculosis. Conditions favoring transsmition of tuberculous The possibility of transmission primarily depends on

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Açikgöz, Mustafa Mert, Ayşem Yurtseven, and Gülsüm Ak. "Primary Tuberculous Lymphadenitis: a Rare Case Report." Balkan Journal of Dental Medicine 22, no. 2 (2018): 106–10. http://dx.doi.org/10.2478/bjdm-2018-0019.

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SummaryBackground/Aim: Our aim is to describe multidisciplinary approach to primary tuberculous lymphadenitis with a case report.Case Report: A 6-year-old boy was referred to İstanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery with the symptoms of painless extra-oral abscess and lymphadenopathy. The diagnosis of primary tuberculous lymphadenitis was proved by microbiological culture and ultrasound imaging.Conclusions: Combine tuberculosis treatment should be applied and long term follow up is necessary. Excisional biopsy for tissue diagnosis and bacterial ex

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Quaranta, Nicola, Paolo Petrone, Alexandra Michailidou, et al. "Tuberculous Otitis Media with Facial Paralysis: A Clinical and Microbiological Diagnosis—A Case Report." Case Reports in Infectious Diseases 2011 (2011): 1–3. http://dx.doi.org/10.1155/2011/932608.

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The tuberculosis of the ear is rare, and in most cases the clinical picture resembles that of a chronic otitis media. The diagnosis is often delayed, and this can lead to irreversible complications such as hearing loss and/or facial paralysis. In view of its rare occurrence, we report a case of primary tuberculous otitis media in a 87-year-old female patient. The diagnosis was made on the basis of both histological and microbiological findings. In particular, gene amplification techniques such as real-time polymerase chain reaction are useful method for rapid diagnosis and detecting tuberculou

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Kashyap, Bineeta, Neha Gupta, Pooja Dewan, NP Singh, and Ashwani Khanna. "Association of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) Grading on the Basis of Cycle Threshold Value with Conventional Microbiological Diagnosis in Pediatric Tuberculosis." Bangladesh Journal of Infectious Diseases 7, no. 2 (2021): 72–77. http://dx.doi.org/10.3329/bjid.v7i2.51513.

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Background: Microbiological confirmation of tuberculosis disease in children remains difficult due to paucibacillary disease and inability to obtain optimal samples. Recently introduced Cartridge based nucleic acid amplification test (CBNAAT) has improved microbiological diagnosis in pediatric tuberculosis. Objectives: We aimed to study association of CBNAAT grading based on cycle threshold value with conventional microbiological diagnosis. Methodology: This prospective study was conducted over a period from November 2016 to October 2017 in the Departments of Microbiology and Pedia

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de Almeida, Sergio Monteiro, Gislene Maria Botão Kussen, Laura Cogo, José Henrique Carvalho, and Keite Nogueira. "Diagnostic characteristics of Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis and rifampicin resistance in Southern Brazil." Arquivos de Neuro-Psiquiatria 78, no. 11 (2020): 700–707. http://dx.doi.org/10.1590/0004-282x20200067.

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ABSTRACT Background: The timely diagnosis of tuberculous meningitis (TBM) is challenging. Molecular diagnostic tools are necessary for TBM, particularly in low- and middle-income countries. Objectives: We aimed to calculate the diagnostics characteristics of Xpert MTB/RIF for the detection of Mycobacterium tuberculosis in the cerebrospinal fluid (CSF) and the frequency of rifampicin (RIF)-resistance in the CSF samples. Methods: A total of 313 consecutive CSF samples were studied and categorized into TBM definite, probable, possible, or not TBM cases based on the clinical, laboratory, and imagi

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Zorkaltseva, E. Yu, L. V. Zaritskaya, and S. V. Pugacheva. "SYMPTOMS OF TUBERCULOSIS IN HIV POSITIVE CHILDREN AND THE IMPACT OF PREVENTION ON THE DEVELOPMENT AND COURSE OF THE DISEASE." Tuberculosis and Lung Diseases 97, no. 3 (2019): 35–39. http://dx.doi.org/10.21292/2075-1230-2019-97-3-35-39.

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The objective of the study: to compare the clinical and X-ray signs of tuberculosis in HIV positive and HIV negative children, to identify factors that are more common in HIV infected children in the region with a high prevalence of both infections. Subjects and methods. 264 children with tuberculosis, and 41 children with HIV infection and tuberculosis undergoing inpatient treatment were enrolled in the study, which was the retrospective study of medical records. The patients underwent standard clinical and X-ray examinations, and microbiological tests. Results. Disseminated forms of tubercul

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Alipour Tehrany, Yassaman, Laurence Toutous-Trellu, Véronique Trombert, Jean-Luc Reny, Gürkan Kaya, and Virginie Prendki. "A Case of Tuberculous Granulomatous Panniculitis without Vasculitis." Case Reports in Dermatology 7, no. 2 (2015): 141–45. http://dx.doi.org/10.1159/000435831.

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We report a case of tuberculous granulomatous panniculitis without vasculitis in an 87-year-old female patient with B-cell chronic lymphocytic leukaemia. One month after starting chemotherapy with chlorambucil and prednisone she presented superficial erythematous plaques on the anterior side of the left leg. Three weeks later erythematous painless deep nodules appeared on the left popliteal fossa and on the left thigh. Cutaneous biopsy revealed granulomatous panniculitis without caseation necrosis or vasculitis. Polymerase chain reaction for Mycobacterium tuberculosis revealed positivity in th

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Sant'Anna, Clemax Couto, Leila de Souza Fonseca, and Maria Helena Féres Saad. "Relação entre o diagnóstico sorológico (ELISA) e a gravidade da tuberculose pulmonar na infância." Revista da Sociedade Brasileira de Medicina Tropical 34, no. 6 (2001): 531–35. http://dx.doi.org/10.1590/s0037-86822001000600006.

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Foram estudadas 48 crianças de 0 a 13 anos através da realização do ensaio imunoenzimático ligado a enzima (ELISA) para pesquisa de anticorpos da classe IgG antiPPD, visando estabelecer correlação entre a resposta imune humoral medida pela sorologia e a gravidade da tuberculose, segundo formas radiológicas (leve, moderada e grave). A amostra foi composta de 29 crianças com tuberculose e 19 sem tuberculose comunicantes de tuberculose). Os valores médios (medianas) da densidade óptica do teste ELISA foram, respectivamente: 0,098 na forma gânglio-pulmonar (leve), 0,092 na forma pneumônica (modera

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Gonzàlez-Martin, Julià. "Microbiología de la tuberculosis." Seminarios de la Fundación Española de Reumatología 15, no. 1 (2014): 25–33. http://dx.doi.org/10.1016/j.semreu.2014.01.001.

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Brant, Cesar Q., Mario R. Silva Jr., Erica P. Macedo, Claudio Vasconcelos, Natalina Tamaki, and M. Lucia G. Ferraz. "The value of adenosine deaminase (ADA) determination in the diagnosis of tuberculous ascites." Revista do Instituto de Medicina Tropical de São Paulo 37, no. 5 (1995): 449–53. http://dx.doi.org/10.1590/s0036-46651995000500011.

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In order to evaluate the role of the determination of adenosine deaminase activity (ADA) in ascitic fluid for the diagnosis of tuberculosis, 44 patients were studied. Based on biochemical, cytological, histopathological and microbiological tests, the patients were divided into 5 groups: G1 - tuberculous ascites (n = 8); G2 - malignant ascites (n = 13); G3 - spontaneous bacterial peritonitis (n = 6); G4 - pancreatic ascites (n = 2); G5 - miscelaneous ascites (n = 15). ADA concentration were significantly higher in G1 (133.50 ± 24.74 U/l) compared to the other groups (G2 = 41.85 ± 52.07 U/l; G3

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Vilar, Fernando Crivelenti, Fábio Fernandes Neves, Jeová Keny Baima Colares, and Benedito Antônio Lopes da Fonseca. "Tuberculose vertebral (doença de Pott) associada a abscesso de psoas: relato de dois casos e revisão da literatura." Revista da Sociedade Brasileira de Medicina Tropical 39, no. 3 (2006): 278–82. http://dx.doi.org/10.1590/s0037-86822006000300011.

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A tuberculose é uma das principais doenças infecciosas de acometimento mundial com 3,9 milhões de casos notificados no mundo, em 2002. A forma esquelética pode corresponder a 3% do número total de casos, sendo 50% devido à tuberculose vertebral. O abscesso de psoas é uma entidade clínica rara com aproximadamente 12 casos relatados por ano na literatura médica e tem no Mycobacterium tuberculosis, um dos seus agentes etiológicos. O objetivo deste trabalho é relatar dois casos de tuberculose vertebral associada a abscesso de psoas atendidos em nosso serviço, bem como uma revisão da literatura.

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Brienze, Vânia Maria Sabadoto, Ângela Pedroso Tonon, Fabrício José Tarelho Pereira, et al. "Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil." Revista da Sociedade Brasileira de Medicina Tropical 34, no. 4 (2001): 389–93. http://dx.doi.org/10.1590/s0037-86822001000400015.

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Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

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Titov, Alexei G., Elena B. Vyshnevskaya, Sergei I. Mazurenko, Seppo Santavirta, and YrjöT Konttinen. "Use of Polymerase Chain Reaction to Diagnose Tuberculous Arthritis From Joint Tissues and Synovial Fluid." Archives of Pathology & Laboratory Medicine 128, no. 2 (2004): 205–9. http://dx.doi.org/10.5858/2004-128-205-uopcrt.

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Abstract Context.—Tuberculosis of the joints and bones is a significant worldwide problem, often leading to joint and bone destruction. The diagnosis of this disease manifestation is difficult. Objective.—To assess the role of conventional diagnostics compared to polymerase chain reaction applied to samples obtained at arthroscopy. Design.—This was an open observational study that was blinded to the microbiologist, histopathologist, and molecular biologist responsible for assessing the main outcome measures. Patients.—Seven patients (8 samples) with joint and bone tuberculosis and 14 patients

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Kulkarni, S. P., M. A. Jaleel, and G. V. Kadival. "Evaluation of an in-house-developed PCR for the diagnosis of tuberculous meningitis in Indian children." Journal of Medical Microbiology 54, no. 4 (2005): 369–73. http://dx.doi.org/10.1099/jmm.0.45801-0.

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Early and rapid detection of the causative organism is necessary in tuberculosis, particularly tuberculous meningitis, as the disease affects mainly children and if untreated or improperly treated can cause severe central nervous system disorders and can often be fatal. An in-house-developed PCR technique was developed for the detection of Mycobacterium tuberculosis DNA, in which the target for amplification was a 340 bp nucleotide sequence located within the 38 kDa protein gene. The test can detect as small an amount of DNA as 10 fg, which is equivalent to two to three organisms, and is highl

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Akhter, Hasina, Zakir Hossain Habib, Imtiaz Ahmed, et al. "Diagnosis of Tuberculous Lymphadenitis from Fine Needle Aspirate by PCR." Bangladesh Journal of Medical Microbiology 8, no. 1 (2017): 2–6. http://dx.doi.org/10.3329/bjmm.v8i1.31051.

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Lymph node involvement is the commonest form of extra pulmonary tuberculosis (EPTB). This study was undertaken to evaluate the role of PCR using IS6110 as a target for Mycobacterium tuberculosis complex in the diagnosis of tuberculous lymphadenitis (TB-L) and was done in Microbiology department of Sir Salimullah Medical College from July 2009 to June 2010. Fine needle aspiration was performed on 107 patients with a clinical suspicion of tuberculous lymphadenitis from different hospitals of Dhaka City Corporation area. All the aspirated samples were subjected to culture and PCR for M.tuberculos

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Klein, H., JB Seeger, and I. Schleicher. "Tuberculous Coxitis: Diagnostic Problems and Varieties of Treatment: A Case Report§." Open Orthopaedics Journal 6, no. 1 (2012): 445–48. http://dx.doi.org/10.2174/1874325001206010445.

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Although the prevalence of tuberculosis reduces, it still belongs to the most important infectious diseases worldwide even in industrial countries. We report an unusual case of tuberculous coxitis in a 28-year-old healthy native female with recurrent hip pain. While X-ray and microbiological examination of the aspirate showed no abnormality, only extended diagnostic measurements and detailed history led to the diagnosis of TBC. Although the patient did not show any pulmonary symptoms open tuberculosis was confirmed. After a course of antibiotic treatment she underwent reconstructive surgery wh

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Ash, C. "MICROBIOLOGY: Containing Tuberculosis." Science 289, no. 5476 (2000): 15a—15. http://dx.doi.org/10.1126/science.289.5476.15a.

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M. AL-Tabbakh, AL-Shaimaa, Rehab S. El Sawy, and Marwa S. EL-Melouk. "Polymorphism in Toll-like Receptor 10 and Tuberculosis Susceptibility in Egyptian Population." EJMM-Volume 30-Issue 2 30, no. 2 (2021): 139–44. http://dx.doi.org/10.51429/ejmm30219.

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Background: One-third of the world’s population is infected with Mycobacterium tuberculosis. Difference in clinical outcome of infection implies that host genetics may be implicated in such variability. Investigations of Toll-like receptors (TLRs) revealed new information regarding the immunopathogenesis of tuberculosis. Toll-like receptor 2 (TLR2) mediates crucial immune response against Mycobacterium tuberculosis. There is argument that Toll-like receptor (TLR10) participate in tuberculosis susceptibility by acting as a signaling modulator for TLR2. Objectives: The aim of this study was inve

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Kiral, Nesrin, Benan Caglayan, Banu Salepci, et al. "Endobronchial ultrasound-guided transbronchial needle aspiration in diagnosing intrathoracic tuberculous lymphadenitis." Medical Ultrasonography 17, no. 3 (2015): 333. http://dx.doi.org/10.11152/mu.2013.2066.173.nki.

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Aims: Patients with suspected tuberculosis without pulmonary lesions and with intrathoracic lymphadenopathy often pose a diagnostic challenge. The aim of this study was to describe the diagnostic utility of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in patients with isolated intrathoracic lymphadenopathy due to tuberculosis (TB). Materials and methods: Cases with tuberculous lymphadenitis (TBLA) as the final diagnosis were analysed among patients in whom EBUS-TBNA had been performed. All patients underwent routine clinical assessment and a CT scan prior to EBU

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Swaim, Laura E., Lynn E. Connolly, Hannah E. Volkman, Olivier Humbert, Donald E. Born, and Lalita Ramakrishnan. "Mycobacterium marinum Infection of Adult Zebrafish Causes Caseating Granulomatous Tuberculosis and Is Moderated by Adaptive Immunity." Infection and Immunity 74, no. 11 (2006): 6108–17. http://dx.doi.org/10.1128/iai.00887-06.

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ABSTRACT The zebrafish, a genetically tractable model vertebrate, is naturally susceptible to tuberculosis caused by Mycobacterium marinum, a close genetic relative of the causative agent of human tuberculosis, Mycobacterium tuberculosis. We previously developed a zebrafish embryo-M. marinum infection model to study host-pathogen interactions in the context of innate immunity. Here, we have constructed a flowthrough fish facility for the large-scale longitudinal study of M. marinum-induced tuberculosis in adult zebrafish where both innate and adaptive immunity are operant. We find that zebrafi

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Maranatha, Daniel, and Samsul Bahri. "Tuberculous Pyopneumothorax as A Complication of Inadequate Treatment in Active Pulmonary Tuberculosis: A Case Report." Journal of Pure and Applied Microbiology 14, no. 2 (2020): 1115–20. http://dx.doi.org/10.22207/jpam.14.2.05.

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Chakravarty, Soumya D., Guofeng Zhu, Ming C. Tsai, et al. "Tumor Necrosis Factor Blockade in Chronic Murine Tuberculosis Enhances Granulomatous Inflammation and Disorganizes Granulomas in the Lungs." Infection and Immunity 76, no. 3 (2008): 916–26. http://dx.doi.org/10.1128/iai.01011-07.

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ABSTRACT Tumor necrosis factor (TNF) is a prototypic proinflammatory cytokine that contributes significantly to the development of immunopathology in various disease states. A complication of TNF blockade therapy, which is used increasingly for the treatment of chronic inflammatory diseases, is the reactivation of latent tuberculosis. This study used a low-dose aerogenic model of murine tuberculosis to analyze the effect of TNF neutralization on disease progression in mice with chronic tuberculous infections. Histological, immunohistochemical, and flow cytometric analyses of Mycobacterium tube

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Gautam, Rupesh, Maria Isabel Atienza, Maika Noda, and Mariaem Andres. "Non-tuberculous Mycobacterium Disease: Radiologic Manifestation in an Infant presenting with Respiratory Distress." Nepalese Journal of Radiology 5, no. 1-2 (2017): 13–20. http://dx.doi.org/10.3126/njr.v5i1-2.18739.

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Non-tuberculous mycobacterium (NTM) comprises distinct group of organisms with lymphadenitis and pulmonary infection as the common manifestation. The diagnosis of pulmonary disease is based on clinical manifestations, radiologic findings and microbiologic culture. The classic NTM infection may be indistinguishable from pulmonary TB. Non-classic infection has predilection to the middle lobe and lingula unlike tuberculosis which is commonly seen in the upper lobes. The disease may also present as hypersensitivity pneumonitis with ground glass like opacities, centrilobular nodules and air trappin

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Ko, Yousang, Changwhan Kim, Yong Park, et al. "Clinical Characteristics and Treatment Outcomes of Definitive versus Standard Anti-Tuberculosis Therapy in Patients with Tuberculous Lymphadenitis." Journal of Clinical Medicine 8, no. 6 (2019): 813. http://dx.doi.org/10.3390/jcm8060813.

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Although it is necessary to culture Mycobacterium tuberculosis from tuberculous lymphadenitis (TBL) patients for definitive therapy, based on the drug-sensitivity test (DST), substantial cases remain culture-negative. Limited data are available regarding the treatment outcomes after standard anti-tuberculosis therapy in culture-negative TBL. The aim of this study was to compare the recurrence rates between definitive anti-tuberculosis therapy, based on DST and standard anti-tuberculosis therapy in culture-negative TBL. A multicenter retrospective cohort study was performed from 2011 to 2015 in

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Che, Nanying, Xinting Yang, Zichen Liu, Kun Li, and Xiaoyou Chen. "Rapid Detection of Cell-Free Mycobacterium tuberculosis DNA in Tuberculous Pleural Effusion." Journal of Clinical Microbiology 55, no. 5 (2017): 1526–32. http://dx.doi.org/10.1128/jcm.02473-16.

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ABSTRACT Tuberculous pleurisy is one of the most common types of extrapulmonary tuberculosis, but its diagnosis remains difficult. In this study, we report for the first time on the detection of cell-free Mycobacterium tuberculosis DNA in pleural effusion and an evaluation of a newly developed molecular assay for the detection of cell-free Mycobacterium tuberculosis DNA. A total of 78 patients with pleural effusion, 60 patients with tuberculous pleurisy, and 18 patients with alternative diseases were included in this study. Mycobacterial culture, the Xpert MTB/RIF assay, the adenosine deaminas

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Gómez Mampaso, E. "Laboratorio de Microbiología y tuberculosis." Revista Clínica Española 202, no. 1 (2002): 16–19. http://dx.doi.org/10.1016/s0014-2565(02)70973-9.

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Lima, Karla Valéria Batista, Maria Luíza Lopes, Edvaldo Carlos Brito Loureiro, et al. "Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar." Revista da Sociedade Brasileira de Medicina Tropical 40, no. 2 (2007): 212–15. http://dx.doi.org/10.1590/s0037-86822007000200013.

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A reação em cadeia da polimerase usada para amplificação de uma seqüência interna de um fragmento previamente amplificado (nested-PCR) foi investigada como uma alternativa complementar a pesquisa de bacilos álcool ácido resistentes e a cultura do Mycobacterium tuberculosis em meio de Lowenstein-Jensen. Foram investigadas 144 amostras de escarro de pacientes suspeitos de tuberculose encaminhados ao Laboratório de Tuberculose do Instituto Evandro Chagas em Belém, no período de junho de 2002 a dezembro de 2003. Das 144 amostras, 121 foram caracterizadas como tuberculose, 119 foram positivas na cu

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Martins, Luiz C., Ilma A. Paschoal, Angela Von Nowakonski, Silvana A. B. Silva, Fernando F. Costa, and Laura Sterian Ward. "Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis." Revista da Sociedade Brasileira de Medicina Tropical 33, no. 3 (2000): 253–57. http://dx.doi.org/10.1590/s0037-86822000000300003.

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Fluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M

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Baveja, C. P., Vidya Nidhi Gumma, Manisha Jain, and Himanshu Jha. "Foot ulcer caused by multidrug-resistant Mycobacterium tuberculosis in a diabetic patient." Journal of Medical Microbiology 59, no. 10 (2010): 1247–49. http://dx.doi.org/10.1099/jmm.0.019554-0.

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Osteoarticular tuberculosis is the fourth leading type of extrapulmonary tuberculosis. The disease has a progressive course and the diagnosis is often made in the later stages of bone destruction. We describe a case of a foot ulcer caused by drug-resistant Mycobacterium tuberculosis in a patient with known diabetes where the diagnosis was not suspected initially. Although tuberculous foot ulcers are rare, they should be included in the differential diagnosis of unknown foot ulcers. A greater awareness of this rare clinical entity may help in commencing specific evidence-based therapy quickly a

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Balasaniantc, G. S., V. V. Dantsev, M. S. Matinina, B. V. Zarecky, and R. D. Muchaidze. "Features of diagnostics of the peripheral lymph nodes tuberculosis." Bulletin of the Russian Military Medical Academy 22, no. 4 (2020): 43–46. http://dx.doi.org/10.17816/brmma62803.

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The analysis of the features of diagnostics of peripheral lymph nodes tuberculosis in modern conditions is carried out. For this 28 case histories of patients suffering from tuberculosis of peripheral lymph nodes were studied and 85,7% of them had a combination of tuberculosis and infection caused by the human immunodeficiency virus. All patients were undergoing biopsies or surgery of lymph nodes, and the material was examined using microbiological molecular, genetic and histological methods. 85,7% of patients had relapses with different periods of time, on average, 34 years, and repeated peri

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Menon, Sarala, Renu Bharadwaj, A. S. Chowdhary, D. V. Kaundinya, and D. A. Palande. "Tuberculous brain abscesses: Case series and review of literature." Journal of Neurosciences in Rural Practice 02, no. 02 (2011): 153–57. http://dx.doi.org/10.4103/0976-3147.83581.

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ABSTRACT Introduction: Tuberculous brain abscess (TBA) is a rare but serious condition. It resembles a pyogenic brain abscess clinically and radiologically and poses a problem in diagnosis and treatment. A final diagnosis is established by smear or culture demonstration of acid fast bacilli (AFB) within the abscess. Here, we report four such cases in our fiveyear study on brain abscesses, along with the different diagnostic modalities used. Materials and Methods: A total of 75 brain abscess pus specimens were collected during neurosurgery, either by burr hole or by craniotomy. These specimens

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Chhajed, Prashant N., Preyas J. Vaidya, Neha P. Mandovra, et al. "EBUS-TBNA in the rapid microbiological diagnosis of drug-resistant mediastinal tuberculous lymphadenopathy." ERJ Open Research 5, no. 4 (2019): 00008–2019. http://dx.doi.org/10.1183/23120541.00008-2019.

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This study aimed to examine the use of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in the rapid diagnosis of mediastinal tuberculous lymphadenitis and drug-resistant mediastinal tuberculous lymphadenitis.A diagnosis of TB was confirmed by a positive Xpert MTB/RIF test or Mycobacterium tuberculosis culture. Rifampicin-resistant TB (RR-TB) or multidrug-resistant TB (MDR-TB) was diagnosed upon the detection of rifampicin resistance by Xpert MTB/RIF or resistance to rifampicin and isoniazid by phenotypic drug susceptibility testing (DST).Xpert MTB/RIF was positive

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Paudel, M. S., Anjana Kafle, Bishal Khatri Chhetri, Sahadev Prasad Dhungana, Anuj Poudel, and Shamsuddhin . "Characteristics of Patients with Tuberculous Pleural Effusion in Rural Nepal." Journal of Lumbini Medical College 1, no. 1 (2013): 31. http://dx.doi.org/10.22502/jlmc.v1i1.10.

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 Introduction: Tuberculosis (TB) is a major cause of mortality and morbidity in developing countries. TubercularPleural effusion is the second most common form of extra pulmonary tuberculosis (EPTB), superseded in Prevalence only by lymph node tuberculosis. Pleural effusion occurs in approximately 5% of patients with TB. The purpose of this study was to assess the demographic characteristics of patients presenting with pleural effusion in rural Nepal.  Methods: A retrospective study was conducted with all the cases diagnosed and admitted with pleural effusion at Lumbini Medic

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Semenova, Nataliya Yu, T. V. Chebotareva, S. N. Orlova, and L. I. Oblogina. "Clinical and morphological characteristics and outcomes of tuberculosis process in patients suffered from HIV infection." Epidemiology and Infectious Diseases 21, no. 6 (2016): 313–21. http://dx.doi.org/10.17816/eid40934.

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The purpose of research is to perform an analysis of features of the clinical course of comorbidity tuberculosis + HIV infection and proximate causes of death of patients. In the article there was performed a comparative analysis of the clinical and radiological, bacteriological and morphological data of 74 deceased patients, divided into two groups: main (cases with the presence of co-infection: tuberculosis associated with HIV infection) and a comparison group (TB mono-infected persons).The performed analysis of the clinical course, the data of microbiological examination and radiographic fi

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