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Journal articles on the topic 'Tubes dilution. eng'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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1

De Simone, Nicole, and Ravi Sarode. "The Use of Activated Partial Thromboplastin Time As a Surrogate to Predict Residual FVIII Activity in the Bethesda Assay." Blood 128, no. 22 (2016): 4962. http://dx.doi.org/10.1182/blood.v128.22.4962.4962.

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Abstract Introduction: Bethesda assays, used in the quantification of factor VIII inhibitors, are time and labor intensive. They are performed by mixing one part of normal pooled plasma (NPP) with an equal amount of serial dilutions of patient plasma in imadizole buffered saline (IBS), and incubating at 37°C for 2 hours. After the incubation time, most laboratories perform factor VIII (FVIII) activities on each tube. These results are divided by the activity resulted on the control (NPP+IBS) to obtain the percent residual activity. The dilution with a percent residual FVIII activity between 25

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Arif, Ani Atul, Tulus Maulana, Ekayanti Mulyawati Kaiin, Bambang Purwantara, Raden Iis Arifiantini, and Erdogan Memili. "Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen." November-2020 13, no. 11 (2020): 2422–28. http://dx.doi.org/10.14202/vetworld.2020.2422-2428.

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Background and Aim: Indonesia has two National Artificial Insemination centers and 17 Regional Artificial Insemination Centers. The frozen semen production techniques differed between the centers, including the type of diluent and semen dilution technique. The aim of the research was to compare the quality of frozen Limousin bull semen diluted using different techniques. Materials and Methods: Semen was collected from three sexually mature Limousin bulls using an artificial vagina. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen that had >70%

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D’Amato, Francesco, Silvia Viciani, Alessio Montori, et al. "Spectroscopic Techniques versus Pitot Tube for the Measurement of Flow Velocity in Narrow Ducts." Sensors 20, no. 24 (2020): 7349. http://dx.doi.org/10.3390/s20247349.

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In order to assess the limits and applicability of Pitot tubes for the measurement of flow velocity in narrow ducts, e.g., biomass burning plants, an optical, dual function device was implemented. This sensor, based on spectroscopic techniques, targets a trace gas, injected inside the stack either in bursts, or continuously, so performing transit time or dilution measurements. A comparison of the two optical techniques with respect to Pitot readings was carried out in different flow conditions (speed, temperature, gas composition). The results of the two optical measurements are in agreement w

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Shiwaku, K., Y. Chigusa, T. Kadosaka, and K. Kaneko. "Factors influencing development of free-living generations of Strongyloides stercoralis." Parasitology 97, no. 1 (1988): 129–38. http://dx.doi.org/10.1017/s0031182000066804.

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SUMMARYThe effects of duration of infection and of temperature and nourishment in cultures on development of free-living generations of Strongyloides stercoralis were studied quantitatively. Rhabditiform larvae, 228–353 μm long, were collected from infected dogs with or without prednisolone treatment using the Baermann apparatus. Cultures were carried out by the filter paper test-tube method under the following conditions: incubation temperature 15–40°C and faecal dilution 1:0–1:16. Rhabditiform larvae developed predominantly to free-living females at incubation temperatures of 15–30°C and low

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Bobev, S. G., J. Van Vaerenbergh, and M. Maes. "First Report of Dickeya dianthicola Causing Blackleg on Potato (Solanum tuberosum) in Bulgaria." Plant Disease 98, no. 2 (2014): 275. http://dx.doi.org/10.1094/pdis-02-13-0147-pdn.

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Potato (Solanum tuberosum) is an important and widespread crop in Bulgaria. A new disease was observed on a single potato plot (Plovdiv region) without a history of potato cultivation in the spring of 2011. Initially, single lower leaves wilted on recently emerged plants (approx. 15% incidence) with subsequent desiccation of the leaf margins. The wilting progressed over time and eventually the whole stem became desiccated. A blackleg-like necrosis was noticed at the stem base when symptomatic plants were uprooted. Most diseased stems remained green above ground but pith tissue was heavily mace

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Kjelland, M. E., T. Stroud, H. E. Ayliffe, and G. Dittami. "178 PORTABLE AUTOMATED MICROFLUIDIC DEVICE FOR RAPID DETERMINATION OF SPERM COUNTS." Reproduction, Fertility and Development 26, no. 1 (2014): 203. http://dx.doi.org/10.1071/rdv26n1ab178.

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The primary objective of this research was to determine whether an automated electronic cell counter using microfluidic technology (Moxi Z) could be effective for determining sperm concentrations. Validation consisted of comparing frozen-thawed conventional bovine sperm counts using the Moxi Z to Coulter Z2 counter results. Specific goals were to determine (1) if the Moxi Z could count intact motile sperm, (2) if differences in media containing egg yolk lipids would affect counting, and (3) evaluate sample concentration requirements for Moxi Z accuracy. Cryopreserved straws (n = 20) of convent

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Loken, Michael R., Sung-Chao Chu, Wayne K. Fritschle, Dian-Kun Li, and Denise A. Wells. "Correcting Flow Cytometric Blast Counts for Blood Contamination in Bone Marrow Aspirates." Blood 110, no. 11 (2007): 4613. http://dx.doi.org/10.1182/blood.v110.11.4613.4613.

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Abstract An accurate blast count is pivotal in the diagnosis, classification and prognosis of patients with myelodysplasia. Blast counts in all previous classification schemes are based on morphologic assessment of marrow aspirates with a poor correlation to blast counts determined by flow cytometry. A significant problem in blast enumeration by flow cytometry is the variable hemodilution of the marrow during collection for flow cytometric analysis. Blast counts can vary depending on which aspirate tube is used for flow analysis, e.g., 2.4%, 1st 5ml tube; 0.62%, 2nd 5ml tube; 0.58% 3rd 5ml tub

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Emerson, David, and Craig L. Moyer. "Neutrophilic Fe-Oxidizing Bacteria Are Abundant at the Loihi Seamount Hydrothermal Vents and Play a Major Role in Fe Oxide Deposition." Applied and Environmental Microbiology 68, no. 6 (2002): 3085–93. http://dx.doi.org/10.1128/aem.68.6.3085-3093.2002.

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ABSTRACT A number of hydrothermal vent sites exist on the summit of the Loihi Seamount, a shield volcano that is part of the Hawaiian archipelago. The vents are 1,100 to 1,325 m below the surface and range in temperature from slightly above ambient (10°C) to high temperature (167°C). The vent fluid is characterized by high concentrations of CO2 (up to 17 mM) and Fe(II) (up to 268 μM), but there is a general paucity of H2S. Most of the vents are surrounded by microbial mats that have a gelatinous texture and are heavily encrusted with rust-colored Fe oxides. Visually, the Fe oxides appeared hom

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Khaeruddin, Khaeruddin, Raden Iis Arifiantini, Cece Sumantri, and Sri Darwati. "KUALITAS SPERMATOZOA AYAM PERANAKAN SENTUL DALAM PENGENCER RINGER LAKTAT KUNING TELUR DENGAN BERBAGAI MONOSAKARIDA (Quality of Sentul Crossbreed Chicken Spermatozoa in Ringer Lactate-Egg Yolk Diluents Supplemented with Various Monosaccharide)." Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences 10, no. 2 (2016): 166–69. http://dx.doi.org/10.21157/j.ked.hewan.v10i2.5090.

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The aim of this study was to examine the preservation of sentul crossbreed chicken semen in ringer lactate egg yolk diluent supplemented with various monosaccharide. Semen was collected from three roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen with more than 70% motility was divided into four tubes. Each of them diluted with ringer lactate egg yolk glucose (RLEYG), ringer lactate egg yolk fructose (RLEYF), ringer lactate egg yolk xylose (RLEYX) and ringer lactate egg yolk mannose (RLEYM). Semen was stored in refri

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Bhakta, Abhijit, Maitreyi Bandyopadhyay, Sayantan Dasgupta, Santanu Sen, Arun Kumar, and Utpal Kumar Biswas. "Effect of NaHS on carbonic anhydrase activity of human erythrocyte." Asian Journal of Medical Sciences 7, no. 3 (2016): 23–27. http://dx.doi.org/10.3126/ajms.v7i3.14047.

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Background: In contrast to its role as poison, hydrogen sulfide (H2S) is recently considered as a gaso-transmitter which mediates important physiologic functions in humans. Evidence is accumulating to demonstrate that inhibitors of H2S production or therapeutic H2S donor compounds exert significant effects in various experimental models. Carbonic anhydrases (CA) are a group of zinc-containing metalloenzymes that catalyse the reversible hydration of carbon dioxide. CAs activity in erythrocytes (CAI and CAII) has recently been observed to be associated with various pathological conditions especi

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AD, Omur. "Antioxidant Potential of Ferulic Acid on the Freezability of Bull Semen." Open Access Journal of Veterinary Science & Research 4, no. 3 (2019): 1–4. http://dx.doi.org/10.23880/oajvsr-16000187.

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Ejaculates were collected twice a week from the bulls, via an artificial vagina, during two weeks. The suitable ejaculates obtained for sperm density (≥ 1.4 × 10 9 spermatoz oa / ml) and for motility (≥ 75%) were used for dilution and freezing of semen. A Tris - based extender (Tris 297.58mM, citric acid 96.32mM, fructose 82.66mM, egg yolk 15% (v/v), glycerol 5% (v/v), gentamicin 0.1 ml / 100ml, pH 6.8 - 7.0) was used as the base extender (cryopreservation diluent). Pooled ejaculate was split into 2 equal aliquots and diluted at 32 °C with base extender containing ferulic acid (100 μM) and no

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Koçak, Mustafa Murat, Sibel Koçak, Elif Aybala Oktay, Abdullah Kiliç, and Sis Darendeliler Yaman. "In vitro Evaluation of the Minimum Bactericidal Concentrations of Different Root-End Filling Materials." Journal of Contemporary Dental Practice 14, no. 3 (2013): 371–74. http://dx.doi.org/10.5005/jp-journals-10024-1330.

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ABSTRACT Aim The aim of this study was to evaluate the minimum bactericidal concentrations (MBC) of root-end filling materials ProRoot MTA, MTA Angelus and IRM. Materials and methods Macrodilution broth method was used. Microorganisms used were: Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212) and Streptococcus mutans. Serial two-fold dilutions of root-end filling samples were prepared in macrodilution tubes with concentrations ranging from 1/2 to 1/512. The samples dilutions were incubated for 24 hours. After incubation, 0.1 ml of diluted culture was inoculated onto the

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Czajkowski, Robert, Waldo J. de Boer, Johannes A. van Veen, and Jan M. van der Wolf. "Downward Vascular Translocation of a Green Fluorescent Protein-Tagged Strain of Dickeya sp. (Biovar 3) from Stem and Leaf Inoculation Sites on Potato." Phytopathology® 100, no. 11 (2010): 1128–37. http://dx.doi.org/10.1094/phyto-03-10-0093.

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Translocation of a green fluorescent protein (GFP)-tagged Dickeya sp. from stems or from leaves to underground parts of potato plants was studied in greenhouse experiments. Thirty days after stem inoculation, 90% of plants expressed symptoms at the stem base and 95% of plants showed browning of internal stem tissue. The GFP-tagged Dickeya sp. was detected by dilution plating in extracts of the stem interiors (100%), stem bases (90%), roots (80%), stolons (55%), and progeny tubers (24%). In roots, the GFP-tagged Dickeya sp. was found inside and between parenchyma cells whereas, in stems and sto

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Journal, Baghdad Science. "The effect of Bacillus thuringiensis in Phthorimaea operculella (Zeller) (Lepetoptera:Gelechiidae)." Baghdad Science Journal 8, no. 1 (2011): 7–12. http://dx.doi.org/10.21123/bsj.8.1.7-12.

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The following dilutions -710X46, -610X46, -510X46 of Bacillus thuringiensis were used for bioassay against the different larval instar of the potato tuber moth Phthorimaea operculella by the spraying method, the results showed that there was no significant influence in the percentage of egg hatching in comparison with the control. The sensitivity of larval stages was reduced with the increasing the age and exposure period. The study also showed that the larvae infected with B.t. stopped feeding, movement and a general paralysis causing the death of larva after (24-48) hours , and the larva col

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15

Yoshinaga, J., Y. Shibata, and M. Morita. "Trace elements determined along single strands of hair by inductively coupled plasma mass spectrometry." Clinical Chemistry 39, no. 8 (1993): 1650–55. http://dx.doi.org/10.1093/clinchem/39.8.1650.

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Abstract Flow injection-inductively coupled plasma mass spectrometry has been evaluated for determining the distribution profile of trace elements along a single strand of hair. Hair was cut into several mm long sections from follicle to the distal end. Each section was solubilized in a capped 1.5-mL polypropylene tube with small volume of nitric acid (typically 50 microL) at room temperature. After dilution an aliquot (50 microL) was introduced into the mass spectrometer by flow injection. The limit of determination was typically 5-50 pg with 5-10% precision (CV), depending on the element exa

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DDesai, Unnati, Vijay Ugale, Trupti Killeker, Meenal Gulve, Swapnil Kolhe, and Gayatri Aher. "A COMPARATIVE EVALUATION OF THE ANTIBACTERIAL AND ANTIFUNGAL EFFICACY OF DIFFERENT ROOT-END FILLING MATERIALS USING TUBE DILUTION METHOD - AN IN VITRO STUDY." International Journal of Advanced Research 7, no. 4 (2019): 541–46. http://dx.doi.org/10.21474/ijar01/8850.

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Hamilton, Martin A., Kelli Buckingham-Meyer, and Darla M. Goeres. "Checking the Validity of the Harvesting and Disaggregating Steps in Laboratory Tests of Surface Disinfectants." Journal of AOAC INTERNATIONAL 92, no. 6 (2009): 1755–62. http://dx.doi.org/10.1093/jaoac/92.6.1755.

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Abstract A chemical disinfectant against surface-associated bacteria typically uses carriers (e.g., glass disks) that are purposely contaminated with bacteria prior to disinfection. After disinfection, the bacteria are harvested by mechanically separating them from the carrier surface to form a suspension of cells in a dilution tube. Bacterial clumps in the tube are disaggregated using mechanical or chemical techniques, thereby creating a well-mixed suspension of single cells suitable for enumeration. Efficacy is quantified by comparing the viable cell count for a disinfected carrier to the vi

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18

Giechaskiel, Zardini, Lähde, Clairotte, Forloni, and Drossinos. "Identification and Quantification of Uncertainty Components in Gaseous and Particle Emission Measurements of a Moped." Energies 12, no. 22 (2019): 4343. http://dx.doi.org/10.3390/en12224343.

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The recent Euro 4 and 5 environmental steps for L-category vehicles (e.g., mopeds, motorcycles) were mainly designed to reduce the emissions of particulate matter and ozone precursors, such as nitrogen oxides and hydrocarbons. However, the corresponding engine, combustion, and aftertreatment improvements will not necessarily reduce the solid particle number (SPN) emissions, suggesting that a SPN regulation may be necessary in the future. At the same time, there are concerns whether the current SPN regulations of passenger cars can be transferred to L-category vehicles. In this study we quantif

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Dantas, Naiara Silva Menezes, Luciene Almeida Siqueira de Vasconcelos, Pedro de Queiroz Costa Neto, and Felipe Faccini dos Santos. "Mold species and fungi load of washed and unwashed table eggs." Acta Veterinaria Brasilica 14, no. 4 (2020): 286–90. http://dx.doi.org/10.21708/avb.2020.14.4.9480.

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Egg quality has been widely studied, mainly because defects in quality can pose risks to public health, as well as economic losses.Nevertheless, studies about fungiin eggsare scarce. The objective was to compare the fungal microbiota from washed and unwashed eggs in the rainy season and dry season of the year. This exploratory research consisted in the analysis of large size white table eggs acquired from 48 different lots. Two manufacturers were sampled considering the main characteristic of washed or unwashed eggs. From each lot, a 30-egg pack were purchased and six of those eggs were used f

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Kpegba, Kafui, Kodjo Eloh, Kodjo Selom Evenamede, et al. "A Comparative Study of the Chemical Composition of the Extracts from Leaves, Stem Bark, and Root Bark of Cassia sieberiana: Antibacterial Activities." Oriental Journal of Chemistry 35, no. 6 (2019): 1678–89. http://dx.doi.org/10.13005/ojc/350608.

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In Togo, the abusive use of the root of Cassia sieberiana D.C. in traditional medicine, contributes gradually to the rarefaction of the species. The general objective of this study is to promote the use of vital organs of Cassia sieberiana in traditional medicine in Togo. The identification of secondary metabolites of the extracts (cyclohexane, dichloromethane and methanol) was carried out by GC-MS and by CL-MS / MS. The antibiotic susceptibility test was performed according to the well diffusion method and the MICs and MBCs according to the tube dilution method. Compounds such as sitosterol α

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Roser, D. J., N. Ashbolt, G. Ho, et al. "Hydrogen sulphide production tests and the detection of groundwater faecal contamination by septic seepage." Water Science and Technology 51, no. 10 (2005): 291–300. http://dx.doi.org/10.2166/wst.2005.0378.

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The ‘H2S test’ is being advanced for microbiological water quality testing where conventional coliform based methods are impractical or too expensive. It involves ambient temperature incubation of water samples with nutrient formulated to generate hydrogen sulphide when ‘faecal’ bacteria are present. Recently a WHO review identified several concerns including the limited number of comparative studies, formulation variability, and false positives and negatives. In response we have compared the H2S test's ability to detect and quantify faecal contamination in an aquifer impacted by septic tank l

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Nethenzheni, L. P., M. L. Mphaphathi, P. V. M. Kalonji, et al. "57 EFFECT OF SEMINAL PLASMA REMOVAL ON SPERM CHARACTERISTICS AND MITOCHONDRIAL MEMBRANE FOLLOWING CRYOPRESERVATION OF SOUTH AFRICAN INDIGENOUS BUCK SEMEN." Reproduction, Fertility and Development 29, no. 1 (2017): 136. http://dx.doi.org/10.1071/rdv29n1ab57.

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The removal of seminal plasma has a significant effect on semen characteristics. The aim of the study was to evaluate the effect of seminal plasma removal on sperm characteristics following semen dilution with Triladyl® or Bioxcell® extenders during cryopreservation. Semen samples were collected from 6 matured South African indigenous bucks for a period of 8 weeks by means of electro-ejaculation. Semen samples were pooled and then divided into 4 aliquots (Triladyl® -washed and non-washed or Bioxcell® -washed and non-washed) and diluted (1:4 vol/vol). Assessment of sperm motility characteristic

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Dalimunthe, Naela Wanda Yusria, M. Rosyid Ridlo, and Agung Budiyanto. "Optimalisasi Pembekuan Sperma Limbah Kauda Epididimis Kambing Lokal dengan Metode Bertahap dan Stabilisasi." Jurnal Sain Veteriner 35, no. 2 (2018): 150. http://dx.doi.org/10.22146/jsv.34663.

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Buck slaugthering produce waste such as testicles including epididymis which contain fertile sperm. Utilization of cauda epididymis as the sources of sperm for producing goat frozen sperm was not reported yet. The aims of this study were improving the frozen-thawed sperm using stabilization and multistep methods which recovered from the waste of buck slaughtering as the source of sperma. Cauda epididymis spermatozoa which was washed then diluted using extender 1 (Tris-citrate-antibiotics) and extender 2 (extender 1- glycerol-egg yolk). The extender 2 addition was performed by single or multist

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Bal, Amandeep, and Christopher P. Chanway. "Evidence of nitrogen fixation in lodgepole pine inoculated with diazotrophic Paenibacillus polymyxa." Botany 90, no. 9 (2012): 891–96. http://dx.doi.org/10.1139/b2012-044.

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Diazotrophic bacteria previously isolated from internal tissues of naturally regenerating lodgepole pine ( Pinus contorta var. latifolia (Dougl.) Engelm.) seedlings were tested for their ability to colonize and fix nitrogen (N) in pine germinants in two experiments. Surface sterilized pine seed was sown in glass tubes containing an autoclaved sand – montmorillonite clay mixture that contained a N-limited nutrient solution labeled with 15N as 0.35 mmol·L–1 Ca(15NO3)2 (5% 15N label). Pine seed was inoculated with one of three of the following bacterial strains: Paenibacillus polymyxa P2b-2R, P.

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Acharya, M., J. M. Burke, C. Hansen, and R. W. Rorie. "21 EVALUATION OF SEMEN EXTENDERS FOR SHORT-TERM STORAGE OF RAM SEMEN AT 4°C." Reproduction, Fertility and Development 29, no. 1 (2017): 118. http://dx.doi.org/10.1071/rdv29n1ab21.

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Preliminary studies found that progressive motility of ram sperm declined ~75% when stored at 4°C for 24 h, and continued to decline over time when using extenders supplemented with 5% egg yolk. The current study evaluated the effects of different combinations of extenders, ethylene glycol (EG), egg yolk, and penicillamine, hypotaurine, and epinephrine on ram sperm progressive motility during storage. Semen collected from 3 Katahdin and 2 Suffolk rams by electroejaculation was distributed across treatment combinations consisting of either TRIS citrate or milk extender supplemented with 5 or 20

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Ustun, N., M. Ozakman, and A. Karahan. "Outbreak of Ralstonia solanacearum Biovar 2 Causing Brown Rot on Potato in the Aegean Region of Turkey." Plant Disease 92, no. 6 (2008): 973. http://dx.doi.org/10.1094/pdis-92-6-0973b.

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Ralstonia solanacearum (Smith) Yabuuchi, Kosako, Yano, Hotta, and Nishiuchi, the cause of brown rot of potato (Solanum tuberosum), was detected for the first time in Turkey in 1995 in five potato fields in the Nevsehir Province of the central Anatolia Region and was eradicated under measures mandated by the government. Occurrence of the pathogen was not reported in other parts of the country. However, in 2006, brown rot symptoms were observed in potato (cv. Marabel) fields in the Balikesir Province of the Aegean Region. Symptoms and signs included wilting, browning of stem vascular tissues, an

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Pelosi, Elvira, Mauro Valtieri, Simona Coppola, et al. "Identification of the hemangioblast in postnatal life." Blood 100, no. 9 (2002): 3203–8. http://dx.doi.org/10.1182/blood-2002-05-1511.

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Abstract Postnatal CD34+ cells expressing vascular endothelial growth factor receptor 2 (KDR) generate hematopoietic or endothelial progeny in different in vitro and in vivo assays. Hypothetically, CD34+KDR+ cells may comprise hemangioblasts bipotent for both lineages. This hypothesis is consistent with 2 series of experiments. In the first series, in clonogenic culture permissive for hematopoietic and endothelial cell growth, CD34+KDR+ cells generate large hemato-endothelial (Hem-End) colonies (5% of seeded cells), whereas CD34+KDR− cells do not. Limiting-dilution analysis indicates that Hem-

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Martin, J. A., J. Figueiredo та L. Wang. "Qualification of a 17β-estradiol (E2) Assay in Sprague Dawley Rat Serum". American Journal of Clinical Pathology 154, Supplement_1 (2020): S85—S86. http://dx.doi.org/10.1093/ajcp/aqaa161.188.

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Abstract Introduction/Objective Significant technical issues are associated with methods used for the measurement of estradiol.The objective of this study was to qualify an electrochemiluminescent (ECL) assay for the quantification of 17β-estradiol (E2) in rat serum. Hemolysis has been identified as a factor that interferes with accurate measurement.The impact of hemolysis was also assessed. Methods Approximately 1.0 mL of whole blood was collected from male and female rats into separate red top tubes and processed to serum. The LoQ for E2 was evaluated by analyzing the low calibrator or at le

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Donadelli, Renan A., Evan C. Titgemeyer, and Charles G. Aldrich. "Organic matter disappearance and production of short- and branched-chain fatty acids from selected fiber sources used in pet foods by a canine in vitro fermentation model1." Journal of Animal Science 97, no. 11 (2019): 4532–39. http://dx.doi.org/10.1093/jas/skz302.

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Abstract Dietary fibers can influence a dog’s overall health, but high concentrations of soluble dietary fibers can cause soft stools. An in vitro model could be useful to predict the rate fibers are fermented once they reach the colon. Pet food companies are constantly searching for new ingredients to differentiate their products from competitors. Miscanthus grass (MG), pea fiber (PF), and sorghum bran (SB) are novel fiber sources that could be alternatives to standards like cellulose (CE) and beet pulp (BP). The objectives of the study were to determine the effects of fiber source on organic

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Marawali, ALoysius, Muhammad S. Abdullah, and Jalaludin Jalaludin. "Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE)." Jurnal Veteriner 20, no. 1 (2019): 20. http://dx.doi.org/10.19087/jveteriner.2019.20.1.20.

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The aim of this research was to know the effectiveness of guava filtrate supplementation in coconut water- egg yolk dilution on quality of liquid semen stored at 5oC of Bali cattle. Semen collected from a five year old Bali cattle using artificial vagina. Semen of good quality were kept in six tubes based on treatment then stored at 5oC. Treatments of the research were P0 : coconut water 80% + egg yolk 20% without guava filtrate; P1 : coconut water 80% + egg yolk 20% + 0.8% guava filtrate; P2 : coconut water 80% + egg yolk 20% + 0.9% guava filtrate; P3 : coconut water 80% + egg yolk 20% + 1.0

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Mustafayeva, G. A. "The study of oleander scale (Aspidiotus nerii Bche) and the way of diluting its entomophages in Azerbaijan." Ecology and Noospherology 25, no. 3-4 (2014): 69–76. http://dx.doi.org/10.15421/031421.

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Scales (Hemiptera, Diaspididae) are the dangerous crop and park-ornamental plant pests. They reproduce very quickly and cause great harm to plants, sometimes even lead to their complete destruction. Scales suck juices from plants, cause premature drying, dying and falling off leaves, dry branches, deformation of leaves, fruits and shoots, reducing annual growth of plants. Therefore the fight with these pests is rather topical in the agriculture. In this connection in this work the analysis of bioecological peculiarities of oleander scale on the territory of Azerbaijan, and also the detection o

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Majumder, Rajib, Irfan A. Rather, Vivek K. Bajpai, and Yong-Ha Park. "In vitro antiviral activity of Lactobacillus plantarum using SPF embryonated eggs and hemagglutination assay." Bangladesh Journal of Pharmacology 10, no. 3 (2015): 688. http://dx.doi.org/10.3329/bjp.v10i3.24475.

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<p>Serum pathogen-free (SPF) embryonated egg for culturing virus has three main steps: a) candling of egg, b) inoculation of egg and c) harvesting of inoculated egg. Furthermore, hemagglutination assay consists of only one step. The method is easy to perform. However, this experiment must be performed in a class 2 biosafety cabinet.</p><p><strong>INTRODUCTION</strong></p><p>More than 200,000 hospitalizations and around 36,000 deaths are associated with influenza infection every year in the United States. As a consequence of consecutive mutations, new i

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Martell, María, Jordi Gómez, Juan I. Esteban, et al. "High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA." Journal of Clinical Microbiology 37, no. 2 (1999): 327–32. http://dx.doi.org/10.1128/jcm.37.2.327-332.1999.

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We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR) that contains a dual-labeled fluorogenic probe to quantify the 5′ noncoding region (5′ NCR) of HCV. The probe contains a fluorescent reporter at the 5′ end and a fluorescent quencher at the 3′ end. The use of such a probe combined with the 5′-3′ nuclease activity of Taq polymerase allows direct qua

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Küsel, Kirsten, Arno Karnholz, Tanja Trinkwalter, Richard Devereux, Georg Acker, and Harold L. Drake. "Physiological Ecology of Clostridium glycolicum RD-1, an Aerotolerant Acetogen Isolated from Sea Grass Roots." Applied and Environmental Microbiology 67, no. 10 (2001): 4734–41. http://dx.doi.org/10.1128/aem.67.10.4734-4741.2001.

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ABSTRACT An anaerobic, H2-utilizing bacterium, strain RD-1, was isolated from the highest growth-positive dilution series of a root homogenate prepared from the sea grass Halodule wrightii. Cells of RD-1 were gram-positive, spore-forming, motile rods that were linked by connecting filaments. Acetate was produced in stoichiometries indicative of an acetyl coenzyme A (acetyl-CoA) pathway-dependent metabolism when RD-1 utilized H2-CO2, formate, lactate, or pyruvate. Growth on sugars or ethylene glycol yielded acetate and ethanol as end products. RD-1 grew at the expense of glucose in the presence

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Ledda, S., J. M. Kelly, S. K. Walker, Y. Natan, and A. Arav. "47 A New Device and Method for Successful Vitrification of In Vitro-Produced Ovine Embryos." Reproduction, Fertility and Development 30, no. 1 (2018): 163. http://dx.doi.org/10.1071/rdv30n1ab47.

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To advance the use of embryo vitrification technology in veterinary practice, we developed a system in which embryo vitrification, warming, and dilution can be performed within a straw. An in-straw embryo cryopreservation method reduces the need for equipment and technical skills and can facilitate direct embryo transfer to the uterus. This study proposes the use of a new device named “Sarah” that is designed to permit all in-straw embryo cryopreservation procedures. Ovine in vitro-produced (IVP) embryos were vitrified at either early blastocyst stage (EB, n = 65, 6 days post-IVF) or fully exp

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Teunis, P., A. Havelaar, J. Vliegenthart, and G. Roessink. "Risk assessment of Campylobacter species in shellfish: identifying the unknown." Water Science and Technology 35, no. 11-12 (1997): 29–34. http://dx.doi.org/10.2166/wst.1997.0705.

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Shellfish are frequently contaminated by Campylobacter spp, presumably originating from faeces from gulls feeding in the growing or relaying waters. The possible health effects of eating contaminated shellfish were estimated by quantitative risk assessment. A paucity of data was encountered necessitating many assumptions to complete the risk estimate. The level of Campylobacter spp in shellfish meat was calculated on the basis of a five-tube, single dilution MPN and was strongly season-dependent. The contamination level of mussels (&lt;1/g) appeared to be higher than in oysters. The usual

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Kwan, Edmond Michael, Sarah To, Heidi Fettke, et al. "Whole blood AR-V7 and AR-V9 mRNA expression and treatment response in metastatic castrate-resistant prostate cancer (mCRPC)." Journal of Clinical Oncology 36, no. 6_suppl (2018): 252. http://dx.doi.org/10.1200/jco.2018.36.6_suppl.252.

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252 Background: Androgen receptor splice variant (AR-V) expression has previously been regarded as a negative predictive biomarker for response to abiraterone and enzalutamide in mCRPC patients. However, recent data questions this association. We designed a whole blood assay to detect AR-V7 and AR-V9, the two most abundantly expressed AR-Vs, and correlated expression with clinical outcomes in patients commencing abiraterone or enzalutamide. Methods: We developed a quantitative real-time polymerase chain reaction assay to detect AR-V7 and AR-V9 from whole blood collected in PAXgene tubes. The a

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Matas, C., J. Gadea, and G. Decuadro-Hansen. "89 EVALUATION OF A CUSHIONED CENTRIFUGATION TECHNIQUE FOR PROCESSING BOAR SEMEN FOR FREEZING." Reproduction, Fertility and Development 17, no. 2 (2005): 195. http://dx.doi.org/10.1071/rdv17n2ab89.

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Boar semen freezing procedures include the use of centrifugation to concentrate sperm and remove seminal plasma prior to dilution in freezing extender. The centrifugation techniques employed have necessarily been a compromise between the need to recover as many spermatozoa as possible after centrifugation and the damage caused by pelleting the sperm. The use of an inert, dense, and isotonic solution as a cushion in the bottom of the tube leads to the use of higher-speed centrifugation to ensure maximum sperm recovery. However, it is necessary to know the viability and functionality of the samp

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Sanchez-Rodriguez, A., M. Arias-Alvarez, P. G. Rebollar, P. L. Lorenzo та R. M. Garcia-Garcia. "148 Inmunolocalization of β-Nerve Growth Factor (NGF) in Male Reproductive Tract and NGF Levels in Serum and Seminal Plasma at Puberty and Adulthood in Rabbit". Reproduction, Fertility and Development 30, № 1 (2018): 214. http://dx.doi.org/10.1071/rdv30n1ab148.

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β-Nerve growth factor (β-NGF) is a neurotrophin with different roles in reproduction that could be regulated by sexual hormone concentrations. The objective of the present study was to characterise β-NGF in the accessory glands and epididymis of male rabbit and to determine whether its expression differentially changed near puberty (week 22) and adulthood (week 37) according to serum testosterone levels and NGF levels in both serum and seminal plasma (SP). Semen and blood were collected every week from 6 males during 3 months and analysed at 3 time points (weeks 22, 30, and 37) by ELISA. The S

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Aiuppa, A., A. Franco, R. von Glasow, et al. "The tropospheric processing of acidic gases and hydrogen sulphide in volcanic gas plumes as inferred from field and model investigations." Atmospheric Chemistry and Physics 7, no. 5 (2007): 1441–50. http://dx.doi.org/10.5194/acp-7-1441-2007.

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Abstract. Improving the constraints on the atmospheric fate and depletion rates of acidic compounds persistently emitted by non-erupting (quiescent) volcanoes is important for quantitatively predicting the environmental impact of volcanic gas plumes. Here, we present new experimental data coupled with modelling studies to investigate the chemical processing of acidic volcanogenic species during tropospheric dispersion. Diffusive tube samplers were deployed at Mount Etna, a very active open-conduit basaltic volcano in eastern Sicily, and Vulcano Island, a closed-conduit quiescent volcano in the

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Aiuppa, A., A. Franco, R. von Glasow, et al. "The tropospheric processing of acidic gases and hydrogen sulphide in volcanic gas plumes as inferred from field and model investigations." Atmospheric Chemistry and Physics Discussions 6, no. 6 (2006): 11653–80. http://dx.doi.org/10.5194/acpd-6-11653-2006.

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Abstract. Improving the constraints on the atmospheric fate and depletion rates of acidic compounds persistently emitted by non-erupting (quiescent) volcanoes is important for quantitatively predicting the environmental impact of volcanic gas plumes. Here, we present new experimental data coupled with modelling studies to investigate the chemical processing of acidic volcanogenic species during tropospheric dispersion. Diffusive tube samplers were deployed at Mount Etna, a very active open-conduit basaltic volcano in eastern Sicily, and Vulcano Island, a closed-conduit quiescent volcano in the

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Carwell, D. B., B. R. Scott, J. Len, et al. "59 EFFECT OF STORAGE DURATION ON POST-THAW PARAMETERS OF BULL SEMEN." Reproduction, Fertility and Development 25, no. 1 (2013): 177. http://dx.doi.org/10.1071/rdv25n1ab59.

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It has been proposed that once good-quality sperm are collected, extended, and cryopreserved that the post-thaw quality will remain high regardless of the duration of storage. A previous study has suggested that there is no effect on post-thaw sperm motility of bovine semen stored in liquid nitrogen up to 13 years. However, no studies have reported the effect of extended storage of bovine semen in LN2. In this study, cryopreserved semen from Angus bulls (n = 25) was utilized. Time frames were assigned to each bull based on the year of collection and cryopreservation: time frame 1 (1960–1975),

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Torres, C. A. A., E. A. Moraes, J. K. Graham, and P. L. Romualdo. "121 ACROSOME REACTION AS EFFECT OF ADDITION OF CHOLESTEROL TO BOAR SPERM MEMBRANES." Reproduction, Fertility and Development 22, no. 1 (2010): 219. http://dx.doi.org/10.1071/rdv22n1ab121.

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Altering the lipid composition of sperm plasma membranes not only affects the ability of sperm capacitation and acrosome reaction, it also affects the way sperm respond to cryopreservation. The objective was to determine if increasing sperm membrane cholesterol levels, by adding cholesterolloaded cyclodextrin (CLC) to boar sperm, alter the cryopreservation sperm to undergo acrosome reaction in vitro. The CLC was prepared as described by Purdy and Graham (2004) with some modification: 200 mg of cholesterol was dissolved in 1 mL of chloroform, and 1 g of methyl-β-cyclodextrin was dissolved in 2

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Plachy, Radek, Lenka Zejskova, Radek Cmejla, et al. "Five-Color Multiplex Real-Time PCR Technology to Detect Over 75 Recurrent Chromosomal Abnormalities in Acute Myeloid Leukemia; Benefits for Minimal Residual Disease Detection." Blood 118, no. 21 (2011): 2526. http://dx.doi.org/10.1182/blood.v118.21.2526.2526.

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Abstract Abstract 2526 Many studies on childhood and adult leukemias have demonstrated that thorough molecular analysis at the time of diagnosis and minimal residual disease (MRD) follow-up are significantly related to the prognosis, and overall- and event-free survival, of patients with acute leukemias. In acute myeloid leukemia (AML), such complex molecular diagnostics and subsequent MRD evaluations are important factors for proper stratification, disease prognosis, assessment of the treatment response, optimal dosage and duration of chemotherapy, and estimation of the optimal timing for hem

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Blevins, B. A., M. de la Rey, and N. M. Loskutoff. "7 EFFECT OF DENSITY GRADIENT CENTRIFUGATION WITH TRYPSIN ON THE FERTILIZING CAPABILITY OF BOVINE SPERM." Reproduction, Fertility and Development 20, no. 1 (2008): 84. http://dx.doi.org/10.1071/rdv20n1ab7.

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The goal of this research was to investigate the effect of a novel density gradient centrifugation (DGC) treatment on the fertilizing capability of bovine sperm as compared to a standard method. Domestic bull (Bos taurus) semen was used for AI and the production of embryos from in vivo-matured bovine oocytes. In 2004, a preliminary study compared the novel semen treatment using a trypsinized PVP-coated silica particle suspension (Percoll; Sigma, St. Louis, MO, USA) to a standard method (4� dilution with an egg yolk diluent) on the fertilizing capacity in vivo of bovine sperm (de la Rey et al.

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Chacur, Marcelo George Mungai, Mariana Grandis Ripari de Souza, Camila Dutra de Souza, and Camila Pires Cremasco. "Effect of Oral Administration of Selenium and Vitamin E on the Quality of Fresh, Refrigerated and Frozen Semen in French Bulldog Breed Dogs." Acta Scientiae Veterinariae 45, no. 1 (2017): 7. http://dx.doi.org/10.22456/1679-9216.79790.

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Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen

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Jiang, Qianli, Shan Jiang, Fanyi Meng, et al. "Efficient Duplex Real-Time Quantitative RT-PCR of BCR-ABL and ABL Gene Transcripts for Monitoring of Minimal Residual Disease." Blood 112, no. 11 (2008): 4887. http://dx.doi.org/10.1182/blood.v112.11.4887.4887.

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Abstract BCL-ABL fusion gene can be found in 100% chronic myelogenous leukemia (CML) and about 25% adult acute lympholid leukemia where its expression level is a crucial parameter for monitoring of minimal residual disease (MRD). Depending on the breakpoint in BCR, exon 2 of ABL (a2) joins with exons 1 (e1), 13 (b2), or 14 (b3), or rarely to exon 19 (e19) of BCR resulting in chimeric proteins of p190, p210 and p230, respectively. The c-ABL gene is one of the best controls for MRD detection by real-time quantitative RT-PCR (RQ-RT-PCR). In most studies published before, PCR probes were labeled b

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Nesrine, Lenchi, Kebbouche Salima, Khelfaoui Mohamed Lamine, et al. "Phylogenetic characterization and screening of halophilic bacteria from Algerian salt lake for the production of biosurfactant and enzymes." World Journal of Biology and Biotechnology 5, no. 2 (2020): 1. http://dx.doi.org/10.33865/wjb.005.02.0294.

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Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by

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Dheda, Shyam, Hongjin Min, David Vesey, Carmel Hawley, David W. Johnson, and Magid Fahim. "Establishing a stable platform for the measurement of blood endotoxin levels in the dialysis population." Diagnosis, April 17, 2020. http://dx.doi.org/10.1515/dx-2019-0088.

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AbstractBackgroundGram-negative lipopolysaccharides are potent inducers of inflammation and have been shown to be present in patients with end-stage kidney disease. There are a variety of different manufacturers and assay types to quantify endotoxin levels; however, there is no standard methodology to demonstrate its presence in plasma.MethodsA control group consisting of haemodialysis and non-kidney disease was selected. Five sets of experiments were conducted to try and ascertain the best platform for plasma endotoxin testing. This included: testing of blank tubes; the effects of freezing, t

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Laich, Andrew R., Jessica Baker, Erik Ninnemann, et al. "Effects of High Fuel Loading and CO2 Dilution on Oxy-Methane Ignition Inside a Shock Tube at High Pressure." Journal of Energy Resources Technology 142, no. 10 (2020). http://dx.doi.org/10.1115/1.4047023.

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Abstract Ignition delay times were measured for methane/O2 mixtures in a high dilution environment of either CO2 or N2 using a shock tube facility. Experiments were performed between 1044 K and 1356 K at pressures near 16 ± 2 atm. Test mixtures had an equivalence ratio of 1.0 with 16.67% CH4, 33.33% O2, and 50% diluent. Ignition delay times were measured using OH* emission and pressure time-histories. Data were compared to the predictions of two literature kinetic mechanisms (ARAMCO MECH 2.0 and GRI Mech 3.0). Most experiments showed inhomogeneous (mild) ignition which was deduced from five ti

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