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Journal articles on the topic 'Tubuline-B'

Author: Grafiati

Published: 4 June 2021

Last updated: 17 February 2022

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1

Živković, S., V. Gavrilović, T. Popović, N. Dolovac, and N. Trkulja. "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia." Plant Disease 98, no. 9 (2014): 1272. http://dx.doi.org/10.1094/pdis-01-14-0052-pdn.

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Quince (Cydonia oblonga Mill.) tree is traditionally grown in Serbia. The fruits are used for compote, marmalade, and brandy production. In December 2012, quince fruits cv. Leskovacka with symptoms of postharvest anthracnose were collected in a storage facility in the area of Sabac, western Serbia. The symptoms were observed on fruits approximately 2 months after harvest. The incidence of the disease was about 3%, but the symptoms were severe. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. Small pieces (3 to 5 mm) of necrotic

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Zhu, Yuanye, Xiaoyu Liang, Yanjun Li та ін. "F240 of β2-Tubulin Explains why Fusarium graminearum is Less Sensitive to Carbendazim than Botrytis cinerea". Phytopathology® 108, № 3 (2018): 352–61. http://dx.doi.org/10.1094/phyto-09-17-0295-r.

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β-Tubulin is the target of benzimidazole fungicides, the most widely used of which is carbendazim (methyl benzimidazol-2-ylcarbamate [MBC]). MBC sensitivity is determined by the differential affinity of MBC for β-tubulins. However, the mechanism of less sensitivity of Fusarium graminearum to MBC compared with other fungi, including Botrytis cinerea, Colletotrichum gloeosporioides, and Sclerotinia sclerotiorum, remains exclusive. Alignment of β-tubulin amino acid sequences showed that position 240 of β-tubulins is leucine (L) in most pathogenic fungi but is phenylalanine (F) in the Fgβ2-tubulin

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3

Vogel, Jacalyn M., Tim Stearns, Conly L. Rieder та Robert E. Palazzo. "Centrosomes Isolated from Spisula solidissima Oocytes Contain Rings and an Unusual Stoichiometric Ratio of α/β Tubulin". Journal of Cell Biology 137, № 1 (1997): 193–202. http://dx.doi.org/10.1083/jcb.137.1.193.

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Centrosome-dependent microtubule nucleation involves the interaction of tubulin subunits with pericentriolar material. To study the biochemical and structural basis of centrosome-dependent microtubule nucleation, centrosomes capable of organizing microtubules into astral arrays were isolated from parthenogenetically activated Spisula solidissima oocytes. Intermediate voltage electron microscopy tomography revealed that each centrosome was composed of a single centriole surrounded by pericentriolar material that was studded with ring-shaped structures ∼25 nm in diameter and &lt;25 nm in len

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4

Gaertig, J., M. A. Cruz, J. Bowen, L. Gu, D. G. Pennock, and M. A. Gorovsky. "Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila." Journal of Cell Biology 129, no. 5 (1995): 1301–10. http://dx.doi.org/10.1083/jcb.129.5.1301.

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In Tetrahymena, at least 17 distinct microtubule structures are assembled from a single primary sequence type of alpha- and beta-tubulin heterodimer, precluding distinctions among microtubular systems based on tubulin primary sequence isotypes. Tetrahymena tubulins also are modified by several types of posttranslational reactions including acetylation of alpha-tubulin at lysine 40, a modification found in most eukaryotes. In Tetrahymena, axonemal alpha-tubulin and numerous other microtubules are acetylated. We completely replaced the single type of alpha-tubulin gene in the macronucleus with a

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5

Tian, Guoling, Sally A. Lewis, Becket Feierbach, et al. "Tubulin Subunits Exist in an Activated Conformational State Generated and Maintained by Protein Cofactors." Journal of Cell Biology 138, no. 4 (1997): 821–32. http://dx.doi.org/10.1083/jcb.138.4.821.

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The production of native α/β tubulin heterodimer in vitro depends on the action of cytosolic chaperonin and several protein cofactors. We previously showed that four such cofactors (termed A, C, D, and E) together with native tubulin act on β-tubulin folding intermediates generated by the chaperonin to produce polymerizable tubulin heterodimers. However, this set of cofactors generates native heterodimers only very inefficiently from α-tubulin folding intermediates produced by the same chaperonin. Here we describe the isolation, characterization, and genetic analysis of a novel tubulin folding

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6

Baffet, Alexandre D., Béatrice Benoit, Jens Januschke, et al. "Drosophila tubulin-binding cofactor B is required for microtubule network formation and for cell polarity." Molecular Biology of the Cell 23, no. 18 (2012): 3591–601. http://dx.doi.org/10.1091/mbc.e11-07-0633.

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Microtubules (MTs) are essential for cell division, shape, intracellular transport, and polarity. MT stability is regulated by many factors, including MT-associated proteins and proteins controlling the amount of free tubulin heterodimers available for polymerization. Tubulin-binding cofactors are potential key regulators of free tubulin concentration, since they are required for α-β–tubulin dimerization in vitro. In this paper, we show that mutation of the Drosophila tubulin-binding cofactor B (dTBCB) affects the levels of both α- and β-tubulins and dramatically destabilizes the MT network in

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7

Gu, W., S. A. Lewis, and N. J. Cowan. "Generation of antisera that discriminate among mammalian alpha-tubulins: introduction of specialized isotypes into cultured cells results in their coassembly without disruption of normal microtubule function." Journal of Cell Biology 106, no. 6 (1988): 2011–22. http://dx.doi.org/10.1083/jcb.106.6.2011.

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To assay the functional significance of the multiple but closely related alpha-tubulin polypeptides that are expressed in mammalian cells, we generated three specific immune sera, each of which uniquely recognizes a distinct alpha-tubulin isotype. All three isotypes are expressed in a tissue-restricted manner: one (M alpha 3/7) only in mature testis, one (M alpha 4) mainly in muscle and brain, and the third (M alpha 6) in several tissues at a very low level. A fourth specific antiserum was also generated that distinguishes between the tyrosinated and nontyrosinated form of a single alpha-tubul

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8

Piperno, G., and M. T. Fuller. "Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms." Journal of Cell Biology 101, no. 6 (1985): 2085–94. http://dx.doi.org/10.1083/jcb.101.6.2085.

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Seven monoclonal antibodies raised against tubulin from the axonemes of sea urchin sperm flagella recognize an acetylated form of alpha-tubulin present in the axoneme of a variety of organisms. The antigen was not detected among soluble, cytoplasmic alpha-tubulin isoforms from a variety of cells. The specificity of the antibodies was determined by in vitro acetylation of sea urchin and Chlamydomonas cytoplasmic tubulins in crude extracts. Of all the acetylated polypeptides in the extracts, only alpha-tubulin became antigenic. Among Chlamydomonas tubulin isoforms, the antibodies recognize only

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9

Uthman, Aumaid U. "Identification and Molecular Characterization of B-Tubulin Gene from Dermatophyte Pathogen Microsporum Canis." Journal of Zankoy Sulaimani - Part A 10, no. 1 (2007): 1–8. http://dx.doi.org/10.17656/jzs.10157.

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10

Geuens, G., A. M. Hill, N. Levilliers, A. Adoutte, and M. DeBrabander. "Microtubule dynamics investigated by microinjection of Paramecium axonemal tubulin: lack of nucleation but proximal assembly of microtubules at the kinetochore during prometaphase." Journal of Cell Biology 108, no. 3 (1989): 939–53. http://dx.doi.org/10.1083/jcb.108.3.939.

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Microtubule (MT) dynamics in PtK2 cells have been investigated using in vivo injection of unmodified Paramecium ciliary tubulin and time-lapse fixation. The sites of incorporation of the axonemal tubulin were localized using a specific antibody which does not react with vertebrate cytoplasmic tubulin (Adoutte, A., M. Claisse, R. Maunoury, and J. Beisson. 1985. J. Mol. Evol. 22:220-229), followed by immunogold labeling, Nanovid microscopy, and ultrastructural observation of the same cells. We confirm data from microinjection of labeled tubulins in other cell types (Soltys, B. J., and G. G. Bori

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11

Piperno, G., M. LeDizet, and X. J. Chang. "Microtubules containing acetylated alpha-tubulin in mammalian cells in culture." Journal of Cell Biology 104, no. 2 (1987): 289–302. http://dx.doi.org/10.1083/jcb.104.2.289.

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The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5-1-2, a monoclonal antibody specific for all alpha-tubulins. The antibody 6-11B-1 binds to primary cilia, centrioles, mitotic spindles, midbodies, and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells, but not in PtK2 cells. These observatio

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12

Samson-Himmelstjerna, G. von. "Anthelminthika-Resistenzen bei Pferde- und Wiederkäuerhelminthen: neueste Forschungsergebnisse aus molekularbiologischer Sicht." Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere 32, no. 06 (2004): 312–15. http://dx.doi.org/10.1055/s-0038-1623506.

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ZusammenfassungGegenwärtig sind Anthelminthika-Resistenzen bei Pferdeund Wiederkäuerhelminthen bereits weit verbreitet. Es handelt sich dabei in erster Linie um Resistenzen gegen Wirkstoffe aus den Gruppen der Benzimidazole (BZ) und bei kleinen Wiederkäuern auch der makrozyklischen Laktone (ML). Das Angriffsziel der BZ ist das Beta-Tubulin. Gemeinsam mit dem Alpha-Tubulin bildet dieses Protein die röhrenartigen Mikrotubulin-Strukturen in den Zellen der Parasiten und Wirte. Die BZ binden an das Beta-Tubulin und blockieren dadurch den Aufbau von Mikrotubuli. Ohne Mikrotubuli können wichtige Zell

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13

Martin-Galiano, Antonio J., María A. Oliva, Laura Sanz, et al. "Bacterial Tubulin Distinct Loop Sequences and Primitive Assembly Properties Support Its Origin from a Eukaryotic Tubulin Ancestor." Journal of Biological Chemistry 286, no. 22 (2011): 19789–803. http://dx.doi.org/10.1074/jbc.m111.230094.

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The structure of the unique bacterial tubulin BtubA/B from Prosthecobacter is very similar to eukaryotic αβ-tubulin but, strikingly, BtubA/B fold without eukaryotic chaperones. Our sequence comparisons indicate that BtubA and BtubB do not really correspond to either α- or β-tubulin but have mosaic sequences with intertwining features from both. Their nucleotide-binding loops are more conserved, and their more divergent sequences correspond to discrete surface zones of tubulin involved in microtubule assembly and binding to eukaryotic cytosolic chaperonin, which is absent from the Prosthecobact

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14

Dhonukshe, Pankaj, Bastiaan O. R. Bargmann та Theodorus W. J. Gadella. "Arabidopsis Tubulin Folding Cofactor B Interacts with α-Tubulin In Vivo". Plant and Cell Physiology 47, № 10 (2006): 1406–11. http://dx.doi.org/10.1093/pcp/pcl001.

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15

Johnson, K. A. "The axonemal microtubules of the Chlamydomonas flagellum differ in tubulin isoform content." Journal of Cell Science 111, no. 3 (1998): 313–20. http://dx.doi.org/10.1242/jcs.111.3.313.

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Little is known of the molecular basis for the diversity of microtubule structure and function found within the eukaryotic flagellum. Antibodies that discriminate between tyrosinated alpha tubulin and post-translationally detyrosinated alpha tubulin were used to localize these complementary tubulin isoforms in flagella of the single-celled green alga Chlamydomonas reinhardtii. Immunofluorescence analysis of intact axonemes detected both isoforms along most of the lengths of flagella; however, each had a short distal zone rich in tyrosinated tubulin. Localizations on splayed axonemes revealed t

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16

Aillaud, Chrystelle, Christophe Bosc, Yasmina Saoudi та ін. "Evidence for new C-terminally truncated variants of α- and β-tubulins". Molecular Biology of the Cell 27, № 4 (2016): 640–53. http://dx.doi.org/10.1091/mbc.e15-03-0137.

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Cellular α-tubulin can bear various carboxy-terminal sequences: full-length tubulin arising from gene neosynthesis is tyrosinated, and two truncated variants, corresponding to detyrosinated and Δ2 α‑tubulin, result from the sequential cleavage of one or two C-terminal residues, respectively. Here, by using a novel antibody named 3EG that is highly specific to the –EEEG C-terminal sequence, we demonstrate the occurrence in neuronal tissues of a new αΔ3‑tubulin variant corresponding to α1A/B‑tubulin deleted of its last three residues (EEY). αΔ3‑tubulin has a specific distribution pattern: its qu

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17

Feierbach, Becket, Eva Nogales, Kenneth H. Downing та Tim Stearns. "Alf1p, a CLIP-170 Domain-containing Protein, Is Functionally and Physically Associated with α-Tubulin". Journal of Cell Biology 144, № 1 (1999): 113–24. http://dx.doi.org/10.1083/jcb.144.1.113.

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Tubulin is a heterodimer of α- and β-tubulin polypeptides. Assembly of the tubulin heterodimer in vitro requires the CCT chaperonin complex, and a set of five proteins referred to as the tubulin cofactors (Tian, F., Y. Huang, H. Rommelaere, J. Vandekerckhove, C. Ampe, and N.J. Cowan. 1996. Cell. 86:287–296; Tian, G., S.A. Lewis, B. Feierbach, T. Stearns, H. Rommelaere, C. Ampe, and N.J. Cowan. 1997. J. Cell Biol. 138:821–832). We report the characterization of Alf1p, the yeast ortholog of mammalian cofactor B. Alf1p interacts with α-tubulin in both two-hybrid and immunoprecipitation assays. Al

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18

Faruki, S., R. L. Geahlen, and D. J. Asai. "Syk-dependent phosphorylation of microtubules in activated B-lymphocytes." Journal of Cell Science 113, no. 14 (2000): 2557–65. http://dx.doi.org/10.1242/jcs.113.14.2557.

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Syk is a protein-tyrosine kinase that is essential for B-lymphocyte development and B-cell signaling. Syk phosphorylates tubulin on tyrosine both in vitro and in intact lymphocytes. Here we show that (alpha)-tubulin present within the cytoskeletal microtubule network was phosphorylated in a Syk-dependent manner following the activation of B-cells by engagement of the B-cell antigen receptor or by treatment with the phosphotyrosine phosphatase inhibitor, pervanadate. Immunofluorescence staining of microtubule cytoskeletons and western blotting studies with antibodies to phosphotyrosine confirme

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19

Schlieper, D., and J. Löwe. "Bacterial tubulin BtubA/B: a folding mystery." Acta Crystallographica Section A Foundations of Crystallography 62, a1 (2006): s24. http://dx.doi.org/10.1107/s0108767306099521.

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20

Luduen̄a, Richard F., Mary Carmen Roach, Veena Prasad, and George R. Pettit. "Interaction of halichondrin B and homohalichondrin B with bovine brain tubulin." Biochemical Pharmacology 45, no. 2 (1993): 421–27. http://dx.doi.org/10.1016/0006-2952(93)90079-c.

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21

Raff, J. W., D. R. Kellogg, and B. M. Alberts. "Drosophila gamma-tubulin is part of a complex containing two previously identified centrosomal MAPs." Journal of Cell Biology 121, no. 4 (1993): 823–35. http://dx.doi.org/10.1083/jcb.121.4.823.

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gamma-tubulin is a minor tubulin that is localized to the microtubule organizing center of many fungi and higher eucaryotic cells (Oakley, B. R., C. E. Oakley, Y. Yoon, and M. C. Jung. 1990. Cell. 61: 1289-1301; Stearns, T., L. Evans, and M. Kirschner. 1991. Cell. 65:825-836; Zheng, Y., M. K. Jung, and B. R. Oakley. 1991. Cell. 65:817-823). Here we show that gamma-tubulin is a component of a previously isolated complex of Drosophila proteins that contains at least two centrosomal microtubule-associated proteins called DMAP190 and DMAP60. Like DMAP190 and DMAP60, the gamma-tubulin in extracts o

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INABA, KAZUO, KOUSAKU OHKAWA, and MASAAKI MORISAWA. "IDENTIFICATION OF TUBULINS ASSOCIATED WITH THE 950 KDA PROTEASE FROM SALMON SPERM ." Biomedical Research 17, no. 1 (1996): 87–93. http://dx.doi.org/10.2220/biomedres.17.87.

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23

Bai, Ruoli, Tam Luong Nguyen, James C. Burnett, et al. "Interactions of Halichondrin B and Eribulin with Tubulin." Journal of Chemical Information and Modeling 51, no. 6 (2011): 1393–404. http://dx.doi.org/10.1021/ci200077t.

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24

Song, Y. H., and E. Mandelkow. "The anatomy of flagellar microtubules: polarity, seam, junctions, and lattice." Journal of Cell Biology 128, no. 1 (1995): 81–94. http://dx.doi.org/10.1083/jcb.128.1.81.

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Although the overall structures of flagellar and cytoplasmic microtubules are understood, many details have remained a matter of debate. In particular, studies of the arrangement of tubulin subunits have been hampered by the low contrast of the tubulin subunits. This problem can now be addressed by the kinesin decoration technique. We have shown previously that the recombinant kinesin head domain binds to beta-tubulin, thus enhancing the contrast between alpha- and beta-tubulin in the electron microscope; this allows one to study the arrangement of tubulin dimers. Here we describe the lattices

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Vinnik, Yuriy, Yulia Belevtsova, and Marina Sadchikova. "PROSPECTS FOR INCREASING THE EFFICIENCY OF TREATMENT OF PATIENTS HEAVING OF LOCALLY- DISTRIBUTED BREAST CANCER." EUREKA: Health Sciences 3 (May 31, 2020): 6–12. http://dx.doi.org/10.21303/2504-5679.2020.001286.

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Improving the efficiency of treatment of locally distributed forms of breast cancer (BC) patients is relevant. The aim of the study: to determine the possibility of increasing the efficiency of treatment of patients with locally distributed BC by supplementing it with the determination of the expression of TOP2 alpha and beta-tubulin III genes in the primary tumor during various chemotherapy regimens. Method. 139 patients with locally distributed BC were examined. Patients received 2-4 courses of neoadjuvant chemotherapy (NAChT) according to the TC (docetaxel + cyclophosphamide) and TAC (docet

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Vee, S., L. Lafanechere, D. Fisher, J. Wehland, D. Job, and A. Picard. "Evidence for a role of the (alpha)-tubulin C terminus in the regulation of cyclin B synthesis in developing oocytes." Journal of Cell Science 114, no. 5 (2001): 887–98. http://dx.doi.org/10.1242/jcs.114.5.887.

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Microinjected mAb YL1/2, an (alpha)-tubulin antibody specific for the tyrosinated form of the protein, blocks the cell cycle in developing oocytes. Here, we have investigated the mechanism involved in the mAb effect. Both developing starfish and Xenopus oocytes were injected with two different (alpha)-tubulin C terminus antibodies. The injected antibodies blocked cell entry into mitosis through specific inhibition of cyclin B synthesis. The antibody effect was independent of the presence or absence of polymerized microtubules and was mimicked by injected synthetic peptides corresponding to the

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Saxton, W. M., and J. R. McIntosh. "Interzone microtubule behavior in late anaphase and telophase spindles." Journal of Cell Biology 105, no. 2 (1987): 875–86. http://dx.doi.org/10.1083/jcb.105.2.875.

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We have studied microtubule behavior in late anaphase and telophase spindles of PtK1 cells, using fluoresceinated tubulin (DTAF-tubulin), microinjection, and laser microbeam photobleaching. We present the results of two novel tests which add to the evidence that DTAF-tubulin closely mimics the behavior of native tubulin in vivo. (a) Microinjected DTAF-tubulin was as effective as injected native tubulin in promoting division of taxol-dependent mitotic mutant cells that had been deprived of taxol. (b) Microinjected colchicine-DTAF-tubulin complex was similar to injected colchicine-native tubulin

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Budiapsari, Putu Indah, I. Kadek Swastika, and Sri Masyeni. "Prevalence of Soil-transmitted Helminths Infection in Students of Klungkung, Bali, after Mass Treatment with AlbendazolePrevalence of Soil-transmitted Helminths Infection in Students of Klungkung, Bali, after Mass Treatment with Albendazole." Open Access Macedonian Journal of Medical Sciences 9, A (2021): 433–39. http://dx.doi.org/10.3889/oamjms.2021.6266.

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 The success of the anti-helminth mass treatment use Albendazole makes detection of soil transmitted helminth infections even more difficult to do microscopically. It is hoped that the molecular method was able to help increase the detectability of Soil Transmitted Helminth infection. The research aimed is to evaluate effectiveness of Albendazole administration in Bali, to identify the presence of β-tubulin gene as molecular diagnosis of STHs infection among children who treated by Albendazole. This study is a cross-sectional study that recruits elementary school children aged 6-12 years

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Schmidt-Cernohorska, M., I. Zhernov, E. Steib, et al. "Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails." Science 363, no. 6424 (2019): 285–88. http://dx.doi.org/10.1126/science.aav2567.

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Microtubule doublets (MTDs), consisting of an incomplete B-microtubule at the surface of a complete A-microtubule, provide a structural scaffold mediating intraflagellar transport and ciliary beating. Despite the fundamental role of MTDs, the molecular mechanism governing their formation is unknown. We used a cell-free assay to demonstrate a crucial inhibitory role of the carboxyl-terminal (C-terminal) tail of tubulin in MTD assembly. Removal of the C-terminal tail of an assembled A-microtubule allowed for the nucleation of a B-microtubule on its surface. C-terminal tails of only one A-microtu

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Pyles, Erica A., and Susan Bane Hastie. "Role of the B-ring substituent in the fluorescence of colchicinoid-tubulin and allocolchicinoid-tubulin complexes." Biochemistry 31, no. 31 (1992): 7086–93. http://dx.doi.org/10.1021/bi00146a009.

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Buendia, B., G. Draetta, and E. Karsenti. "Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase." Journal of Cell Biology 116, no. 6 (1992): 1431–42. http://dx.doi.org/10.1083/jcb.116.6.1431.

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Isolated centrosomes nucleate microtubules when incubated in pure tubulin solutions well below the critical concentration for spontaneous polymer assembly (approximately 15 microM instead of 60 microM). Treatment with urea (2-3 M) does not severely damage the centriole cylinders but inactivates their ability to nucleate microtubules even at high tubulin concentrations. Here we show that centrosomes inactivated by urea are functionally complemented in frog egg extracts. Centrosomes can then be reisolated on sucrose gradients and assayed in different concentrations of pure tubulin to quantify th

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Kikkawa, M., T. Ishikawa, T. Nakata, T. Wakabayashi, and N. Hirokawa. "Direct visualization of the microtubule lattice seam both in vitro and in vivo." Journal of Cell Biology 127, no. 6 (1994): 1965–71. http://dx.doi.org/10.1083/jcb.127.6.1965.

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Microtubules are constructed from alpha- and beta-tubulin heterodimers that are arranged into protofilaments. Most commonly there are 13 or 14 protofilaments. A series of structural investigations using both electron microscopy and x-ray diffraction have indicated that there are two potential lattices (A and B) in which the tubulin subunits can be arranged. Electron microscopy has shown that kinesin heads, which bind only to beta-tubulin, follow a helical path with a 12-nm pitch in which subunits repeat every 8-nm axially, implying a primarily B-type lattice. However, these helical symmetry pa

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Silverman-Gavrila, R. V., and A. Forer. "Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes." Journal of Cell Science 113, no. 4 (2000): 597–609. http://dx.doi.org/10.1242/jcs.113.4.597.

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We studied the effects of various drugs on the poleward flux of tubulin in kinetochore microtubules in metaphase-I crane-fly spermatocytes. We used as a measure of tubulin flux a ‘gap’ in acetylation of kinetochore microtubules immediately poleward from the kinetochore; the ‘gap’ is caused by a time lag between incorporation of new tubulin subunits at the kinetochore and subsequent acetylation of those subunits as they flux to the pole. We confirmed that the ‘gap’ is due to flux by showing that the ‘gap’ disappeared when cells were treated briefly with the anti-tubulin drug nocodazole, which d

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Królewska-Golińska, Karolina, Marcin J. Cieślak, Milena Sobczak, et al. "Novel Benzo[B]Furans with Anti-Microtubule Activity Upregulate Expression of Apoptotic Genes and Arrest Leukemia Cells in G2/M Phase." Anti-Cancer Agents in Medicinal Chemistry 19, no. 3 (2019): 375–88. http://dx.doi.org/10.2174/1871520619666181122123552.

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Background:Novel derivatives of benzo[b]furan were found to be highly toxic towards human chronic myelogenous (K562), acute myelogenous (HL-60) and acute lymphoblastic (MOLT-4) leukemia cells.Objective:The objective was the characterization of the biological activity of novel benzofurans (influence on apoptosis, mitogen-activated protein kinases and on the cell cycle). Cellular protein(s) targeted by test benzofurans and mechanism of action were identified.Methods:The methods utilized in the study were chemical synthesis, fluorescence assays, flow cytometry, gene expression by DNA microarray a

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Shpetner, H. S., B. M. Paschal, and R. B. Vallee. "Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C)." Journal of Cell Biology 107, no. 3 (1988): 1001–9. http://dx.doi.org/10.1083/jcb.107.3.1001.

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We recently found that the brain cytosolic microtubule-associated protein 1C (MAP 1C) is a microtubule-activated ATPase, capable of translocating microtubules in vitro in the direction corresponding to retrograde transport. (Paschal, B. M., H. S. Shpetner, and R. B. Vallee. 1987b. J. Cell Biol. 105:1273-1282; Paschal, B. M., and R. B. Vallee. 1987. Nature [Lond.]. 330:181-183.). Biochemical analysis of this protein (op. cit.) as well as scanning transmission electron microscopy revealed that MAP 1C is a brain cytoplasmic form of the ciliary and flagellar ATPase dynein (Vallee, R. B., J. S. Wal

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Larsson, Niklas, Bo Segerman, Bonnie Howell, Kajsa Fridell, Lynne Cassimeris, and Martin Gullberg. "Op18/Stathmin Mediates Multiple Region-Specific Tubulin and Microtubule-Regulating Activities." Journal of Cell Biology 146, no. 6 (1999): 1289–302. http://dx.doi.org/10.1083/jcb.146.6.1289.

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Oncoprotein18/stathmin (Op18) is a regulator of microtubule (MT) dynamics that binds tubulin heterodimers and destabilizes MTs by promoting catastrophes (i.e., transitions from growing to shrinking MTs). Here, we have performed a deletion analysis to mechanistically dissect Op18 with respect to (a) modulation of tubulin GTP hydrolysis and exchange, (b) tubulin binding in vitro, and (c) tubulin association and MT-regulating activities in intact cells. The data reveal distinct types of region-specific Op18 modulation of tubulin GTP metabolism, namely inhibition of nucleotide exchange and stimula

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Blume, R. Ya, A. N. Rabokon та Ya V. Pirko. "β-tubulin intron length polymorphism among forms var. glabra and var. laxa of napa cabbage". Faktori eksperimental'noi evolucii organizmiv 26 (1 вересня 2020): 87–92. http://dx.doi.org/10.7124/feeo.v26.1247.

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Aim. Main aim of this research was identification of genetic distances between different genotypes of napa cabbage (B. rapa ssp. pekinensis) and diversity identification in var. glabra and var. laxa forms. Methods. Molecular genetic analysis of napa cabbage genotypes was conducted out using method of β-tubulin intron length polymorphism (TBP). Results. Molecular profiles of different napa cabbage (B. rapa ssp. pekinensis) genotypes were identified. Number of amplified β-tubulin intron fragments was significantly varying – from 12 to 24 for each genotype. Basing on obtained results a dendrogram

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Waterhouse, N. J., J. Oliaro та M. J. Pinkoski. "A ‘polarized’ look at α-tubulin cleavage by granzyme B". Cell Death & Differentiation 13, № 11 (2006): 1839–41. http://dx.doi.org/10.1038/sj.cdd.4402023.

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Ota, Shintaro, Shougo Tomioka, Haruki Sogawa, et al. "Binding properties between curcumin and malarial tubulin: molecular-docking and ab initio fragment molecular orbital calculations." Chem-Bio Informatics Journal 18 (2018): 44–57. http://dx.doi.org/10.1273/cbij.18.44.

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Boyé, O., A. Brossi, H. J. C. Yeh, E. Hamel, B. Wegrzynski, and V. Toome. "Natural products. Antitubulin effect of congeners of N-acetylcolchinyl methyl ether: synthesis of optically active 5-acetamidodeaminocolchinyl methyl ether and of demethoxy analogues of deaminocolchinyl methyl ether." Canadian Journal of Chemistry 70, no. 5 (1992): 1237–49. http://dx.doi.org/10.1139/v92-160.

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Trimethoxy-substituted dihydrodibenzocycloheptenes 4–7, required for a structure–activity study measuring the inhibition of tubulin polymerization in vitro, were synthesized by four different routes: (1) Synthesis of 4 was achieved from 2,3-dimethoxybenzaldehyde via biphenyl aldehyde 17, chain lengthening to propionic acid 20, acid-catalyzed cyclization toward ketone 21, and removal of the carbonyl group. (2) Compound 5 was obtained by eliminating the sterically most hindered methoxy group in 25 or 26 by metal reduction in alcohol. (3) Compound 6 was prepared from biphenyl aldehyde 34 obtained

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Al-Karmalawy, Ahmed A., and Muhammad Khattab. "Molecular modelling of mebendazole polymorphs as a potential colchicine binding site inhibitor." New Journal of Chemistry 44, no. 33 (2020): 13990–96. http://dx.doi.org/10.1039/d0nj02844d.

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Olenieva, V. D., D. I. Lytvyn, A. I. Yemets, and Ya B. Blume. "Expression profiling of kinesins, involved in the development of autophagy in Arabidopsis thaliana, and the role of tubulin acetylation in the interaction of Atg8 protein with microtubules." Faktori eksperimental'noi evolucii organizmiv 22 (September 9, 2018): 162–68. http://dx.doi.org/10.7124/feeo.v22.942.

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Aim. To investigate the interrelation between changes in the expression levels of kinesin genes that are potentially involved in the development of stress-induced autophagy in Arabidopsis thaliana by means of microtubules, and the structural biology analysis of the role of α-tubulin acetylation in the regulation of interaction of α-tubulin with Atg8. Methods. The simulation of the influence of abiotic stresses. PCR analysis of changes in expression levels of kinesin genes. The molecular dynamics simulations of α-tubulin and Atg8 complexes were performed using the GROMACS 4.5.5 program. Results

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Pathak, Narendra, Tomoko Obara, Steve Mangos, Yan Liu, and Iain A. Drummond. "The Zebrafish fleer Gene Encodes an Essential Regulator of Cilia Tubulin Polyglutamylation." Molecular Biology of the Cell 18, no. 11 (2007): 4353–64. http://dx.doi.org/10.1091/mbc.e07-06-0537.

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Cilia and basal bodies are essential organelles for a broad spectrum of functions, including the development of left-right asymmetry, kidney function, cerebrospinal fluid transport, generation of photoreceptor outer segments, and hedgehog signaling. Zebrafish fleer (flr) mutants exhibit kidney cysts, randomized left-right asymmetry, hydrocephalus, and rod outer segment defects, suggesting a pleiotropic defect in ciliogenesis. Positional cloning flr identified a tetratricopeptide repeat protein homologous to the Caenorhabditis elegans protein DYF1 that was highly expressed in ciliated cells. fl

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Oegema, Karen, Christiane Wiese, Ona C. Martin та ін. "Characterization of Two Related Drosophila γ-tubulin Complexes that Differ in Their Ability to Nucleate Microtubules". Journal of Cell Biology 144, № 4 (1999): 721–33. http://dx.doi.org/10.1083/jcb.144.4.721.

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γ-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1– 12). Here, we report the purification and characterization of both complexes that we name γ-tubulin small complex (γTuSC; ∼280,000 D) and Drosophila γTuRC (∼2,200,000 D). In addition to γ-tubulin, the γTuSC contains Dgrip84 and Dgrip91, two proteins homologous to the Spc97/98p protein family. The γTuSC is a structural subunit of the γTuRC, a larger complex containing about six additional polypeptides. Like the γTuRC isolated from Xenopus egg ext

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Caron, J. M., A. L. Jones, and M. W. Kirschner. "Autoregulation of tubulin synthesis in hepatocytes and fibroblasts." Journal of Cell Biology 101, no. 5 (1985): 1763–72. http://dx.doi.org/10.1083/jcb.101.5.1763.

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Microtubule polymer levels in mouse 3T6 fibroblasts and primary cultures of rat hepatocytes can be manipulated by treatment of cells with long term, low doses of colcemid. Such treatment produces a rather uniform population of cells with microtubules of reduced lengths. Using this system, we demonstrate (a) that the rate of tubulin synthesis is sensitive to small changes (10%) in microtubule polymer mass and (b) that the percent of inhibition of synthesis is proportional to the level of soluble tubulin. Experiments with hepatocytes indicate that not only synthesis but the stability of tubulin

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Wang, Xiaojuan, Mine Tanaka, Herbenya Silva Peixoto, and Michael Wink. "Cucurbitacins: elucidation of their interactions with the cytoskeleton." PeerJ 5 (May 30, 2017): e3357. http://dx.doi.org/10.7717/peerj.3357.

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Cucurbitacins, a class of toxic tetracyclic triterpenoids in Cucurbitaceae, modulate many molecular targets. Here we investigated the interactions of cucurbitacin B, E and I with cytoskeletal proteins such as microtubule and actin filaments. The effects of cucurbitacin B, E and I on microtubules and actin filaments were studied in living cells (Hela and U2OS) and in vitro using GFP markers, immunofluorescence staining and in vitro tubulin polymerization assay. Cucurbitacin B, E and I apparently affected microtubule structures in living cells and cucurbitacin E inhibited tubulin polymerization

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Ito, Yuko, Akiko Kanamaru, and Akihiro Tada. "A novel agent, methylophiopogonanone B, promotes Rho activation and tubulin depolymerization." Molecular and Cellular Biochemistry 297, no. 1-2 (2006): 121–29. http://dx.doi.org/10.1007/s11010-006-9336-y.

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Schlieper, D., M. A. Oliva, J. M. Andreu, and J. Lowe. "Structure of bacterial tubulin BtubA/B: Evidence for horizontal gene transfer." Proceedings of the National Academy of Sciences 102, no. 26 (2005): 9170–75. http://dx.doi.org/10.1073/pnas.0502859102.

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Sarkar, Taradas, Tam Luong Nguyen, Zhi-Wei Su, et al. "Interaction of pseudolaric acid B with the colchicine site of tubulin." Biochemical Pharmacology 84, no. 4 (2012): 444–50. http://dx.doi.org/10.1016/j.bcp.2012.05.014.

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Tong, Yun-Guang, Xiong-Wen Zhang, Mei-Yu Geng, et al. "Pseudolarix Acid B, a New Tubulin-Binding Agent, Inhibits Angiogenesis by Interacting with a Novel Binding Site on Tubulin." Molecular Pharmacology 69, no. 4 (2006): 1226–33. http://dx.doi.org/10.1124/mol.105.020537.

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