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Journal articles on the topic 'TUNEL'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Avdić, Adam, Kemal Gutić, Ivan Mlakić Vuković, and Sanel Alić. "TEHNOLOGIJA ISKOPA CESTOVNOG TUNELA HRANJEN MEHANIZOVANIM KONCEPTOM." Nauka+Praksa 27 (June 6, 2024): 57–68. http://dx.doi.org/10.62683/nip27.57-68.

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Bosna i Hercegovina je zemlja u kojoj uglavnom preovladava brdsko planiniski reljef, pa izrada nove putne infrastrukture ne predstavlja nimalo jednostavan zadatak, odnosno u manjoj ili većoj mjeri ovisno od konfiguracije terena nameće se kao potreba stalna izrada nadzemnih i podzemnih objekata kao što su mostovi, vijadukti i tuneli. Izrada cestovnog tunela Hranjen kao sastavnog dijela buduće brze ceste Prača-Hranjen-Goražde u istočnom dijelu zemlje predstavlja jedan od najzahtjevnijih projekata posljednih decenija, a u momentu završetka izrade sa dužinom od preko 5.500,0 m tunel Hranjen predst

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Blagojević, Predrag, and Miljan Milenković. "GLAVNI PROJEKAT TUNELA NA PUTU GUSAR - LAZA U AZERBEJDŽANU." Nauka+Praksa 16 (October 10, 2013): 71–78. http://dx.doi.org/10.62683/nip16.71-78.

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U radu je opisan projekat i proračun konstrukcije tunela na deonici autoputa od grada Gusar do grada Laza u Azerbejdžanu. Autoput prolazi kroz nacionalni park i skijaški centar. Da se ne bi narušilo prirodno okruženje postojećih i novoprojektovanih hotela prokopan je tunel u dužini od 340.37m koji je predmet ovog rada. Tunel je armiranobetonski, sastoji iz deset segmenata međusobno dilatiranih. Širina tunela je 10.2m a visina 6.0m. Debljina donje ploče je 1,35-1,45m, gornje ploče 1,17-1,30m, zidovi su debljine 1,40m.

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3

Komór, Kinga, Magda Kołaczyńska, and Dominika Kosylak. "Drążenie tuneli technologią TBM." BUILDER 262, no. 5 (2019): 100–102. http://dx.doi.org/10.5604/01.3001.0013.3569.

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W artykule została scharakteryzowana metoda wykonania tunelu z wykorzystaniem technologii TBM. Na przykładzie linii metra warszawskiego zaprezentowany został schemat wykonywania prac ta metoda oraz monitoring bezpieczenstwa przylegajacej infrastruktury. Metoda ta jest równie z powodzeniem wykorzystywana do draenia tuneli drogowych, wodociagowych, kanalizacyjnych, hydroenergetycznych czy wielozadaniowych. Za pomoca technologii TBM utworzono ju 369 projektów tuneli metra o długosci ponad 880 km, 440 km tuneli kolejowych i 171 km drogowych. Łacznie ta technologia wydraono ponad 1900 km tuneli

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Rucińska, Teresa, Janusz Kozanecki, and Maciej K. Kokolus. "Technologia mrożenia gruntupodczas budowy tunelu pod Świną." Przegląd Budowlany 94, no. 9-10 (2023): 6–10. http://dx.doi.org/10.5604/01.3001.0053.9353.

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Budowa tuneli komunikacyjnych to ogromne wyzwaniedla kadry inżynieryjnej. Tunel pod Martwą Wisłą w Gdańskuoraz pod Świną, łączący wyspy Uznam i Wolin, dowodzą, jakwymagające są tego typu inwestycje, nie tylko z uwagi na kosztyczy ich realizację, ale również zarządzanie budową. To takżekonieczność korzystania z nowoczesnych technologii, które wymagająnajwyższych kompetencji od wszystkich uczestnikówprocesu inwestycyjno-budowlanego, a zakończona budowa tunelupod Świną jest tego dowodem. Realizacja tej inwestycji wymagałazastosowania metody pozwalającej na wykonanie wyjśćawaryjnych poza obudową t

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Flaga, Andrzej, and Łukasz Flaga. "Laboratorium Inżynierii Wiatrowej L-14– Tunele aerodynamiczne do badań środowiskowychi budowlanych." Przegląd Budowlany 96, no. 3 (2025): 70–72. https://doi.org/10.5604/01.3001.0055.1427.

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Laboratorium Inżynierii Wiatrowej PolitechnikiKrakowskiej (LIW PK) to unikalna w skali Polski i ceniona międzynarodowojednostka badawczo-dydaktyczna, specjalizująca sięw badaniach z zakresu aerodynamiki budowli, inżynierii śniegowej,energetyki wiatrowej oraz inżynierii środowiska. Początkowofunkcjonowało z jednym tunelem aerodynamicznym, otwartymw 2001 roku, natomiast w 2024 roku oddano do użytku nowykompleks z trzema dodatkowymi tunelami. Całość inwestycji zostałazrealizowana w ramach projektu „Budowa Laboratorium AerodynamikiŚrodowiskowej Politechniki Krakowskiej”. Podstawoweprzeznaczenie po

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6

Gergeta Sotončić, Kristina. "Rezultati arheoloških radova uz poligonalnu kulu u Carrarinoj ulici u Puli 2021. godine." Histria, no. 11 (December 30, 2021): 11–28. http://dx.doi.org/10.32728/h2021.01.

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U veljači 2021. arheološki je istražen prostor u polovici širine poligonalne kule uz ulaz u tunel Zerostrasse i ulazni dio tunela u Carrarinoj ulici u Puli. Povod tomu bila je zamjena kabelskoga voda za dizalo koje će povezivati mrežu podzemnih tunela i utvrdu Kaštel. Istraživanjem je utvrđena fazna izgrađenost kule: kasnoantička kula poligonalnoga oblika, čija je struktura vidljiva u današnjoj hodnoj razini, nalegla je na antičku kulu kružnoga oblika, koja je temeljena na četvrtastoj kuli iz najranije faze formiranja pulskoga gradskog obrambenog sustava. Na samom ulazu u tunel otkriveni su os

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Gergeta Sotončić, Kristina. "Rezultati arheoloških radova uz poligonalnu kulu u Carrarinoj ulici u Puli 2021. godine." Histria, no. 11 (December 30, 2021): 11–28. http://dx.doi.org/10.32728/h2021.01.

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U veljači 2021. arheološki je istražen prostor u polovici širine poligonalne kule uz ulaz u tunel Zerostrasse i ulazni dio tunela u Carrarinoj ulici u Puli. Povod tomu bila je zamjena kabelskoga voda za dizalo koje će povezivati mrežu podzemnih tunela i utvrdu Kaštel. Istraživanjem je utvrđena fazna izgrađenost kule: kasnoantička kula poligonalnoga oblika, čija je struktura vidljiva u današnjoj hodnoj razini, nalegla je na antičku kulu kružnoga oblika, koja je temeljena na četvrtastoj kuli iz najranije faze formiranja pulskoga gradskog obrambenog sustava. Na samom ulazu u tunel otkriveni su os

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8

Gergeta Sotončić, Kristina. "Rezultati arheoloških radova uz poligonalnu kulu u Carrarinoj ulici u Puli 2021. godine." Histria, no. 11 (December 30, 2021): 11–28. http://dx.doi.org/10.32728/h2021.01.

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U veljači 2021. arheološki je istražen prostor u polovici širine poligonalne kule uz ulaz u tunel Zerostrasse i ulazni dio tunela u Carrarinoj ulici u Puli. Povod tomu bila je zamjena kabelskoga voda za dizalo koje će povezivati mrežu podzemnih tunela i utvrdu Kaštel. Istraživanjem je utvrđena fazna izgrađenost kule: kasnoantička kula poligonalnoga oblika, čija je struktura vidljiva u današnjoj hodnoj razini, nalegla je na antičku kulu kružnoga oblika, koja je temeljena na četvrtastoj kuli iz najranije faze formiranja pulskoga gradskog obrambenog sustava. Na samom ulazu u tunel otkriveni su os

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9

., Supriya, Sneha G. Kalthur, and Guruprasad Kalthur. "Cellular Changes and Effect on DNA Integrity of the Epididymal Cells of Swiss Albino Mice Post Exposure to High Dose of Cyclophosphamide: A Histological and TUNEL Assay Study." International Journal of Science and Healthcare Research 7, no. 1 (2022): 54–64. http://dx.doi.org/10.52403/ijshr.20220111.

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Background: Many studies done in past literature focuses on gonadal toxicity of Cyclophosphamide, but very less studies are done on the epididymis post exposure to this drug. Hence a sincere attempt is made in present study to look into the cellular changes in the epididymis through histology and TUNEL Assay. Subjects & Methods: Healthy male Swiss albino adult mice, were obtained from Central Animal Research Facility, Manipal were used for the study. The adult as well as prepubertal male mice were divided into control and test groups. In test group, the mice were injected with Cyclophospha

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10

Negoescu, A., P. Lorimier, F. Labat-Moleur, et al. "In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations." Journal of Histochemistry & Cytochemistry 44, no. 9 (1996): 959–68. http://dx.doi.org/10.1177/44.9.8773561.

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TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) is a method of choice for rapid identification and quantification of the apoptotic cell fraction in cultured cell preparations. However, TUNEL application has been restricted to a narrow spectrum of sample conditions, and only detergents have been proposed as labeling enhancers. This study was aimed at extending TUNEL to variously fixed cells and improving TUNEL sensitivity by optimized pretreatments, the specificity being assessed by reference to the apoptotic morphology. Comparative TUNEL was performed with three p

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Moore, Christopher L., Alena V. Savenka, and Alexei G. Basnakian. "TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation." International Journal of Molecular Sciences 22, no. 1 (2021): 412. http://dx.doi.org/10.3390/ijms22010412.

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Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3’-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell death in cultured kidney cells and animal and human kidneys resulting from toxic or hypoxic injury. The early characterization of TUNEL as an apoptotic assay has led to numerous misinterpretations of the mechanisms of kidney cell injury. Nevertheless, TUNEL is becoming

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12

Masiakowska-Osses, Dorota. "Nie do przebycia? O granicach i ich przekraczaniu w powieści "Tunel" Magdaleny Parys." Acta Neophilologica 2, no. XXIII (2021): 229–40. http://dx.doi.org/10.31648/an.6668.

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This article is an analysis of Tunel (The Tunnel), the novel by Magdalena Parys, a Polish author living in Germany. The action of the book focuses on the fictional construction of an escape tunnel built under the border wall between the western and eastern part of Berlin in the early 1980s. This, somehow, predestines Parys’s work to be read in the context of transgression, and crossing limitations. Starting with the writer’s non-literary inspirations, the article shows the multiplicity of literal and metaphorical meanings of the term “border” and examines how the motif functions in the present

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13

Wu, Yi-Chun, Gillian M. Stanfield, and H. Robert Horvitz. "NUC-1, a Caenorhabditis elegans DNase II homolog, functions in an intermediate step of DNA degradation during apoptosis." Genes & Development 14, no. 5 (2000): 536–48. http://dx.doi.org/10.1101/gad.14.5.536.

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One hallmark of apoptosis is the degradation of chromosomal DNA. We cloned the Caenorhabditis elegans gene nuc-1, which is involved in the degradation of the DNA of apoptotic cells, and found that nuc-1 encodes a homolog of mammalian DNase II. We used the TUNEL technique to assay DNA degradation in nuc-1 and other mutants defective in programmed cell death and discovered that TUNEL labels apoptotic cells only during a transient intermediate stage. Mutations in nuc-1 allowed the generation of TUNEL-reactive DNA but blocked the conversion of TUNEL-reactive DNA to a subsequent TUNEL-unreactive st

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Chegini, Raziye, Mahshad Khodarahmian, Niloufar Ahmadian, et al. "Evaluation of Sperm DNA Fragmentation in Oligoasthenoteratozoospermia Patients Using Two Different Techniques: TUNEL and Sperm Chromatin Dispersion Assays." Galen Medical Journal 13 (October 8, 2024): e3515. http://dx.doi.org/10.31661/gmj.v13i.3515.

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Background: Oligoasthenoteratozoospermia (OAT) is the most prevalent male infertility condition that is mainly caused by sperm DNA fragmentation (SDF). This study compared the sensitivity and effectiveness of two different approaches for analyzing SDF in patients with OAT: sperm chromatin dispersion (SCD) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). Materials and Methods: In this study, which received ethical committee approval, participants were divided in to normal and OAT groups (n=20 for each). both TUNEL and SCD assays were used to analyze the sperm DNA fragme

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Masetto, Tathiana Elisa, and José Marcio Rocha Faria. "In situ DNA fragmentation during the re-establishment of desiccation tolerance in germinated seeds of Cedrela fissilis Vell." Journal of Seed Science 41, no. 2 (2019): 244–49. http://dx.doi.org/10.1590/2317-1545v42n2207417.

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Abstract: Dehydration is a necessary procedure prior to exposing seeds to long term storage, but this is associated with metabolism-linked injury mediated by cell injury. In order to assess cellular alterations during re-establishment of desiccation tolerance (DT) in C. fissilis germinated seeds and their relation to DNA damage, we verified the occurrence of DNA fragmentation through the TUNEL test and its evidence through the cytological analyses. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moist

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Mirzayans, Razmik, and David Murray. "Do TUNEL and Other Apoptosis Assays Detect Cell Death in Preclinical Studies?" International Journal of Molecular Sciences 21, no. 23 (2020): 9090. http://dx.doi.org/10.3390/ijms21239090.

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The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay detects DNA breakage by labeling the free 3ʹ-hydroxyl termini. Given that genomic DNA breaks arise during early and late stages of apoptosis, TUNEL staining continues to be widely used as a measure of apoptotic cell death. The advantages of the assay include its relative ease of performance and the broad availability of TUNEL assay kits for various applications, such as single-cell analysis of apoptosis in cell cultures and tissue samples. However, as briefly discussed herein, aside from some concerns relat

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Chambers, Steven A., Mathew Newman, Melissa M. Frangie, Alena V. Savenka, Alexei G. Basnakian, and Mohammad A. Alam. "Antimelanoma activities of chimeric thiazole–androstenone derivatives." Royal Society Open Science 8, no. 8 (2021): 210395. http://dx.doi.org/10.1098/rsos.210395.

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The discovery of chimeric anti-melanoma agents is reported. These molecules are potent growth suppressors of melanoma cells in vitro with growth inhibition of 50% (GI 50 ) values as low as 1.32 µM. Compounds were more toxic to melanoma cells in vitro than commonly used anti-melanoma agent dacarbazine as measured by TUNEL assay. They induced both caspase-independent apoptosis evident by colocalization of TUNEL with endonuclease G (EndoG) and caspase-mediated apoptosis measured by colocalization of TUNEL with caspase-activated DNase (CAD). In addition, compounds 3 and 5 strongly induced oxidativ

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M-Alicante, Sara, Arantza Muguruza-Montero, Óscar R. Ballesteros, Ane Metola, Alvaro Villarroel, and Eider Nuñez. "Proteinen tolestura tunel erribosomikoan." EKAIA Euskal Herriko Unibertsitateko Zientzia eta Teknologia Aldizkaria, no. 43 (September 13, 2022): 101–16. http://dx.doi.org/10.1387/ekaia.23624.

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Proteinak polimero lineal gisa sintetizatzen dira eta beren jatorrizko egitura tridimentsionalean tolestu behar dira zelulan hainbat funtzio betetzeko. Proteinen tolespena ulertzea funtsezkoa da, tolespen okerrak hainbat gaixotasun neuro-degeneratiboren jatorria direlako. Proteinen tolespena modu koitzultzailean has daiteke, hau da, sortzen ari den peptidoa erribosomari lotuta dagoenean oraindik. Izan ere, zelularen proteinen heren bat baino gehiago erribosomaren tunelaren espazio mugatuan tolesten direla frogatu da, hau da, erribosomaren gainazalarekiko interakzioek modulatuta eta erribosoma-

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Kelly, K. J., Ruben M. Sandoval, Kenneth W. Dunn, Bruce A. Molitoris, and Pierre C. Dagher. "A novel method to determine specificity and sensitivity of the TUNEL reaction in the quantitation of apoptosis." American Journal of Physiology-Cell Physiology 284, no. 5 (2003): C1309—C1318. http://dx.doi.org/10.1152/ajpcell.00353.2002.

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Apoptosis is an important mode of cell death under both physiological and pathophysiological conditions. Numerous techniques are available for the study and quantitation of apoptosis in cell culture, but only few are useful when applied to complex tissues. Among these, the terminal transferase-mediated dUTP nick end-labeling (TUNEL) assay remains the most widely used technique. However, its specificity and sensitivity for the detection of apoptosis remain controversial. We developed a technique consisting of staining live cells and tissues with Hoechst 33342 and the vital dye propidium iodide

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Isailović, Slobodan. "MIKROMREŽA TUNELA "STRAŽEVICA" U RESNIKU (BEOGRAD)." Zbornik radova Fakulteta tehničkih nauka u Novom Sadu 35, no. 02 (2020): 360–63. http://dx.doi.org/10.24867/06kg02isailovic.

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Tunel je najčešće saobraćajni ili hidrotehnički objekat koji se nalazi u zemljištu, na jednom ili oba kraja izlazi na površinu, ili uopšte ne izlazi na površinu. U radu je opisan projekat i realizacija projekta mikromreže za tunel "Straževica" u Beogradu.

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Umemura, S., M. Yasuda, R. Y. Osamura, Y. Kawarada, T. Sugiyama, and Y. Tsutsumi. "Enhancement of TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method using mung bean nuclease, a single-stranded DNA digestion enzyme." Journal of Histochemistry & Cytochemistry 44, no. 2 (1996): 125–32. http://dx.doi.org/10.1177/44.2.8609368.

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The TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method has been employed widely to demonstrate apoptotic cells in routinely prepared paraffin sections. Because the apoptotic cells were reactive with the antibody to single-stranded DNA, we attempted to enhance the TUNEL positivity by pretreatment with single-stranded DNA digestion enzymes, S1 nuclease, and mung bean nuclease. When mung bean nuclease (5 U/50 microliter/section) was incubated at 37 degrees C for 30 min, the TUNEL reaction was most effectively enhanced. Pretreatment with S1 nuclease (0.25 U/50 microliter/section) at 37 degr

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Ragosta, Maria Emanuela, Giulia Traini, Lara Tamburrino, et al. "Sperm Chromatin Dispersion Test Detects Sperm DNA Fragmentation Mainly Associated with Unviable Spermatozoa and Underestimates the Values with Respect to TUNEL Assay." International Journal of Molecular Sciences 25, no. 8 (2024): 4481. http://dx.doi.org/10.3390/ijms25084481.

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Several clinical laboratories assess sperm DNA fragmentation (sDF) in addition to semen analysis in male infertility diagnosis. Among tests evaluating sDF, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) and SCD (Sperm Chromatin Dispersion) are widely used. Our lab developed a modified version of TUNEL (TUNEL/PI) able to distinguish two sperm populations (PI Brighter and PI Dimmer) differently associated with sperm viability and reproductive outcomes. The aim of this study was to compare sDF levels detected by SCD and TUNEL/PI in the semen samples from 71 male subjects att

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Dagistanli, F., H. Ozkaya, B. Kucukyoruk, et al. "Preoperative Somatostatin Analogue Treatment Might Trigger Apoptosis and Autophagy in Tumor Tissues of Patients with Acromegaly: A Pilot Study." Experimental and Clinical Endocrinology & Diabetes 126, no. 03 (2016): 168–75. http://dx.doi.org/10.1055/s-0042-107243.

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Abstract Objective: To evaluate the effect of preoperative somatostatin analog (SRL) treatment on proteins associated with apoptosis and autophagy in patients with acromegaly and to determine factors correlating with these parameters. Methods: Ex-vivo tumor samples of 11 SRL-treated and 9 SRL-untreated patients were retrospectively included in the study. Apoptotic and autophagic proteins were determined via immunohistochemical staining and apoptosis was evaluated via in situ DNA end labeling (TUNEL). Results: TUNEL, caspase-3, and ATG-5 immunopositivity was significantly increased (p<0.01,

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Britton, Mark, Jose Rafols, Sarah Alousi, and Joseph C. Dunbar. "The Effects of Middle Cerebral Artery Occlusion on Central Nervous System Apoptotic Events in Normal and Diabetic Rats." Experimental Diabesity Research 4, no. 1 (2003): 13–20. http://dx.doi.org/10.1080/15438600303727.

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Apoptosis and neural degeneration are characteristics of cerebral ischemia and brain damage. Diabetes is associated with worsening of brain damage following ischemic events. In this study, the authors characterize the influence of focal cerebral ischemia, induced by middle cerebral artery occlusion, on 2 indexes of apoptosis,TUNEL(terminal deoxynucleotidyl transferase–mediated deoxyuridine 5-triphosphate nick end-labeling) staining and caspase- 3 immunohistochemistry. Diabetes was induced in normal rats using streptozotocin and maintained for 5 to 6 weeks. The middle cerebral artery of both no

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Meiyanto, Edy. "PENINGKATAN EKSPRESI p53 OLEH EKSTRAK ETANOLIK RUMPUT MUTIARA (Hedyotis corymbosa) PADA SEL HEPAR TIKUS SPRAGUE DAWLEY TERINDUKSI 7,12-DIMETILBENZ[a]ANTRASENA." Pharmacon: Jurnal Farmasi Indonesia 11, no. 1 (2015): 1–6. http://dx.doi.org/10.23917/pharmacon.v11i1.62.

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Asam ursolat dan asam oleanolat yang terdapat dalam Rumput Mutiara (Hedyotis corymbosa) diduga dapat menghambat kanker dengan berbagai mekanisme. Penelitian ini dirancang untuk mengetahui kemampuan ekstrak etanolik rumput mutiara sebagai agen pemacu apoptosis melalui uji in vivo menggunakan tikus galur Sprague Dawley terinduksi 7,12-dimetilbenz[a]antrasena (DMBA). Kelompok hewan uji yang digunakan terdiri dari kontrol ekstrak 1500mg/kgBB, kontrol pelarut CMC-Na, kontrol DMBA, perlakuan DMBA+ekstrak dosis 750mg/kgBB dan perlakuan DMBA+ekstrak 1500mg/kgBB. Hasil percobaan selanjutnya dianalisis

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Zorc-Pleskovič, R., A. Alibegović, M. Zorc, A. Milutinović, N. Radovanović, and Daniel Petrovič. "Apoptosis of Cardiomyocytes in Myocarditis." Folia Biologica 52, no. 1-2 (2006): 6–9. https://doi.org/10.14712/fb2006052010006.

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Apoptosis of cardiomyocytes has been reported to be involved in the pathogenesis of heart failure of different aetiologies. The purpose of this study is to assess the role and extent of apoptosis of cardiomyocytes in active myocarditis. Endomyocardial samples from the right ventricle of 22 patients with active myocarditis were compared with 25 traffic accident victims without a history of cardiovascular disease. Twenty-two patients fulfilled the histopathologic Dallas criteria for myocarditis. The TUNEL method and immunostaining for active caspase 3 were used for the detection of apoptosis. Im

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Chatzimeletiou, Katerina, Alexandra Fleva, Theodoros-Thomas Nikolopoulos, et al. "Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry." Medicina 59, no. 7 (2023): 1313. http://dx.doi.org/10.3390/medicina59071313.

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Background and Objectives: Sperm DNA fragmentation refers to any break in one or both of the strands of DNA in the head of a sperm. The most widely used methodologies for assessing sperm DNA fragmentation are the sperm chromatin structure assay (SCSA), the sperm chromatin dispersion assay (SCD), the single-cell gel electrophoresis assay (SCGE–comet), and the terminal-deoxynucleotidyl-transferase (TdT)-mediated dUTP nick end labelling (TUNEL) assay. The aim of this study was to compare the efficiency and sensitivity of the analysis of sperm DNA fragmentation using TUNEL via fluorescence microsc

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Sloop, Gregory D., Juan C. Roa, Alberto G. Delgado, John T. Balart, Merrill O. Hines, and James M. Hill. "Histologic Sectioning Produces TUNEL Reactivity." Archives of Pathology & Laboratory Medicine 123, no. 6 (1999): 529–32. http://dx.doi.org/10.5858/1999-123-0529-hsptr.

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Abstract Objective.—To determine if the DNA strand breaks caused by tissue sectioning result in terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end-labeling (TUNEL) reactivity. Methods.—The incidence and location of TUNEL-positive nuclei were determined in 5- and 15-μm sections of human stomach. Five- and 15-μm sections of tonsil were stained as a positive control. Results.—In 5-μm gastric sections, 69% of nuclei were labeled; in 15-μm sections, only 30% were labeled. In the latter sections, almost all labeled nuclei were located at the cut surface of sections. La

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Machado, D. A., and W. P. Martins. "Síndrome do tunel do carpo." Experts in Ultrasound: Reviews and Perspectives 1, no. 3 (2009): 136–40. http://dx.doi.org/10.4281/eurp.2009.03.02.

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Obradović, Nadežda, and Vanja Bulić. "Tunel: Lepa sela lepo gore." World Literature Today 72, no. 1 (1998): 168. http://dx.doi.org/10.2307/40153659.

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Short, Ben. "TUNEL vision spots apoptotic cells." Journal of Cell Biology 208, no. 1 (2015): 7. http://dx.doi.org/10.1083/jcb.2081fta.

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Walker, J. A., and P. Quirke. "Viewing apoptosis through a ?TUNEL?" Journal of Pathology 195, no. 3 (2001): 275–76. http://dx.doi.org/10.1002/path.979.

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SATOH, Kasumi, Tetsuo NAGATANI, and Hiroshi NAKAJIMA. "Examination of BCC by TUNEL method. Examination of apotosis in BCC by TUNEL method." Skin Cancer 13, no. 2 (1998): 154–57. http://dx.doi.org/10.5227/skincancer.13.154.

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Pini, Taylor, Rachel Makloski, Karen Maruniak, William B. Schoolcraft, and Mandy G. Katz-Jaffe. "Mitigating the Effects of Oxidative Sperm DNA Damage." Antioxidants 9, no. 7 (2020): 589. http://dx.doi.org/10.3390/antiox9070589.

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Sperm DNA damage is correlated with reduced embryo development and increased miscarriage risk, reducing successful conception. Given its links with oxidative stress, antioxidants have been investigated as a potential treatment, yet results are conflicting. Importantly, individual antioxidants are not identical in composition, and some compounds may be more effective than others. We investigated the use of the polyphenol-rich, high-antioxidant-capacity fruit acai as a treatment for elevated sperm DNA fragmentation (>16%), measured by terminal deoxynucleotidyl transferase dUTP nick end labell

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Shabisgh, Ahmad, Nozomu Tanji, Vivette D’Agati, et al. "Early Effects of Castration on the Vascular System of the Rat Ventral Prostate Gland*." Endocrinology 140, no. 4 (1999): 1920–26. http://dx.doi.org/10.1210/endo.140.4.6644.

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Abstract Recent studies have found that blood flow to the rat ventral prostate gland is drastically reduced at an early time after castration. These observations caused us to reevaluate the effects of castration on the various cell populations of the ventral prostate, especially those in the prostatic vascular system. Sections of ventral prostate glands obtained at different times after castration were analyzed using the TUNEL (terminal deoxynucleotide transferase-mediated dUTP nick END labeling) staining method to quantify apoptosis in different cell types. The results of this analysis showed

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KRISHNAMURTHY, HANUMANTHAPPA, GERHARD E. WEINBAUER, HUMAIRA ASLAM, CHING‐HEI YEUNG, and EBERHARD NIESCHLAG. "Quantification of Apoptotic Testicular Germ Cells in Normal and Methoxyacetic Acid‐Treated Mice as Determined by Flow Cytometry." Journal of Andrology 19, no. 6 (1998): 710–17. http://dx.doi.org/10.1002/j.1939-4640.1998.tb02080.x.

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ABSTRACT: Several studies have reported the occurrence and significance of programmed ceil death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by morphological criteria or by in situ end labeling (TUNEL) and were enumerated from histological sections by semi‐quantitative and time‐consuming techniques. In the present study, we have established a flow cytometric technique for quantification of TUNEL‐positive cells in the mouse testis. Groups of five adult mice each received 0, 650, or 1300 mg/kg (IP) of methoxyacetic acid (MAA), and teste

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McCall, Cordero L., James Stinson, Ryan W. Dobbs, et al. "Abstract A079: Genetic ancestry and vitamin D may predict degree of prostatic apoptosis in prostate tumor and benign epithelium among men undergoing radical prostatectomy." Cancer Epidemiology, Biomarkers & Prevention 32, no. 1_Supplement (2023): A079. http://dx.doi.org/10.1158/1538-7755.disp22-a079.

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Abstract There is evidence that vitamin D metabolites such as 25 hydroxyvitamin D [25(OH)D], may be protective against prostate cancer (PCa). Vitamin D and its analogs are hypothesized to have pro-apoptotic effects in multiple cell lines. Blacks have higher rates of vitamin D deficiency, higher tumor progression rates 1,2, and higher apoptosis rates3 compared to Whites. West African ancestry (WAA) may confound this relationship if it is associated with both vitamin D status and apoptosis rates. Our objective is to assess for independent associations between in vivo vitamin D status, genetic an

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SATO, TOSHINOBU, MIENEKE G. A. VAN DIXHOORN, FRANS A. PRINS, et al. "The Terminal Sequence of Complement Plays an Essential Role in Antibody-Mediated Renal Cell Apoptosis." Journal of the American Society of Nephrology 10, no. 6 (1999): 1242–52. http://dx.doi.org/10.1681/asn.v1061242.

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Abstract. Mesangial cell (MC) injury is a characteristic feature in the early phase of Thy.1 nephritis. The present study investigates the contribution of complement to MC apoptosis in this experimental model of kidney disease in rats. Thy.1 nephritis was induced by injection of mouse anti-Thy.1 monoclonal antibody (ER4G). To assess the contribution of the terminal sequence of complement on apoptosis, the studies were performed in complement-sufficient PVG/c (PVG/c+) rats and in rats deficient in complement C6 (PVG/c-). Apoptosis was monitored by assessment of the number of condensed nuclei in

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Prunell, Giselle F., Niels-Aage Svendgaard, Kanar Alkass, and Tiit Mathiesen. "Delayed cell death related to acute cerebral blood flow changes following subarachnoid hemorrhage in the rat brain." Journal of Neurosurgery 102, no. 6 (2005): 1046–54. http://dx.doi.org/10.3171/jns.2005.102.6.1046.

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Object. The authors tested the hypotheses that subarachnoid hemorrhage (SAH) leads to delayed cell death with the participation of apoptotic-like mechanisms and is influenced by the degree of acute decrease in the cerebral blood flow (CBF) following hemorrhage. Methods. Subarachnoid hemorrhage was induced in rats by endovascular perforation of the internal carotid artery or injection of blood into the prechiasmatic cistern. Cerebral blood flow was measured using laser Doppler flowmetry for 60 minutes. Brain sections stained with terminal deoxynucleotidyl transferase-mediated deoxyuridine triph

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Sun, Yun, Changman Zhou, Paula Polk, Anil Nanda, and John H. Zhang. "Mechanisms of Erythropoietin-induced Brain Protection in Neonatal Hypoxia-Ischemia Rat Model." Journal of Cerebral Blood Flow & Metabolism 24, no. 2 (2004): 259–70. http://dx.doi.org/10.1097/01.wcb.0000110049.43905.ac.

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Erythropoietin, a hemotopoietic growth factor, has brain protective actions. This study investigated the mechanisms of Recombinant Human EPO (rhEPO)-induced brain protection in neonates. An established rat hypoxia-ischemia model was used by ligation of the right common carotid artery of 7-day-old pups, followed by 90 minute of hypoxia (8% 02 and 92% N2) at 37°C. Animals were divided into three groups: control, hypoxia-ischemia, and hypoxia-ischemia plus rhEPO treatment. In rhEPO treated pups, 300 units rhEPO was administered intraperitoneally 24 hours before hypoxia. rhEPO treatment (300 units

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Bikbova, Guzel, Toshiyuki Oshitari, Takayuki Baba, and Shuichi Yamamoto. "Combination of Neuroprotective and Regenerative Agents for AGE-Induced Retinal Degeneration: In Vitro Study." BioMed Research International 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/8604723.

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To determine the most effective combination of neuroprotective and regenerative agents for cultured retinal neurons from advanced glycation end products- (AGEs-) induced degeneration, retinal explants of 7 adult Sprague-Dawley rats were three-dimensionally cultured in collagen gel and incubated in serum-free media and in 7 media; namely, AGEs, AGEs + 100 μM citicoline, AGEs + 10 ng/mL NT-4, AGEs + 100 μM TUDCA, AGEs + 100 μM citicoline + TUDCA (doublet), and AGEs + 100 μM citicoline + TUDCA + 10 ng/mL NT-4 (triplet) were examined. The number of regenerating neurites was counted after 7 days of

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Kunz, Tina, Niklas Marklund, Lars Hillered, and Ernst H. Oliw. "Effects of the selective cyclooxygenase-2 inhibitor rofecoxib on cell death following traumatic brain injury in the rat." Restorative Neurology and Neuroscience 24, no. 1 (2006): 55–63. https://doi.org/10.3233/rnn-2006-00330.

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Purpose: Lateral fluid percussion brain injury (FPI) increases cyclooxygenase-2 (COX-2) expression in the cortex and hippocampus. The objective was to investigate whether the selective COX-2 inhibitor rofecoxib (10 mg/kg twice daily) reduces neuronal cell death after FPI in rats, since rofecoxib has been shown to be neuroprotective in other models of CNS injury. Methods: Rofecoxib (n=23) or vehicle (n=20) were administered after FPI and for up to 3 days. Cell death was evaluated by Fluoro-Jade B staining and by the TdT-mediated dUTP nick end labelling (TUNEL) assay. Results: COX-2 immunoreacti

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Matsushita, Kohji, Wei Meng, Xiaoying Wang, et al. "Evidence for Apoptosis After Intracerebral Hemorrhage in Rat Striatum." Journal of Cerebral Blood Flow & Metabolism 20, no. 2 (2000): 396–404. http://dx.doi.org/10.1097/00004647-200002000-00022.

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The overall hypothesis that cell death after intracerebral hemorrhage is mediated in part by apoptotic mechanisms was tested. Intracerebral hemorrhage was induced in rats using stereotactic infusions of 0.5 U of collagenase (1-μL volume) into the striatum. After 24 hours, large numbers of TUNEL-positive stained cells with morphologies suggestive of apoptosis were present in the center and periphery of the hemorrhage. Double staining with Nissl and immunocytochemical labeling with antibodies against neuronal nuclei and glial fibrillary acidic protein suggested that these TUNEL-positive cells we

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Grigaitienė, Jūratė, Irena Marčiukaitienė, Audra Blažienė, and Anželika Chomičienė. "Study of apoptosis in normal skin tissues." Medicina 43, no. 4 (2007): 301. http://dx.doi.org/10.3390/medicina43040037.

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The aim of the study was to investigate the apoptosis in normal human skin by examination of all epidermal layers. Material and methods. The normal skin epidermis of 15 healthy subjects was investigated by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-X nick end labeling) technique. Apoptotic cells were evaluated in the germinative and differential compartments and stratum corneum. Only highly TUNEL-positive cells with typical morphological DNA fragmentation signs were calculated. Results. In vital strata (except stratum corneum) of normal skin epidermis, 37.5% of all TUNEL-positi

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Maciejewska, Beata, Małgorzata Lipowska, Przemysław Kowiański, Beata Domaradzka-Pytel, and Janusz Moryś. "Postnatal development of the rat striatum – a study using in situ DNA end labeling technique." Acta Neurobiologiae Experimentalis 58, no. 1 (1998): 23–28. http://dx.doi.org/10.55782/ane-1998-1255.

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We have examined the development of rat striatum for evidence of cells dying in the process of physiological cell death. In present study we have indicated apoptotic cells in sections stained with cresyl violet (cell death characterized by pyknosis) or with DNA end labeling assay (TUNEL method). Our results demonstrated that cell loss during maturation of the rat striatum had the characteristics of apoptosis rather than necrosis. The greatest number of TUNEL - positive and pyknotic cells in the striatum were found during the first postnatal days; after 7th day of postnatal life a rapid decreas

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Loureiro, Bárbara, Amber Mary Brad та Peter James Hansen. "Heat shock and tumor necrosis factor-α induce apoptosis in bovine preimplantation embryos through a caspase-9-dependent mechanism". Reproduction 133, № 6 (2007): 1129–37. http://dx.doi.org/10.1530/rep-06-0307.

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Heat shock and tumor necrosis factor-α (TNF-α) induce apoptosis through different mechanisms, with heat shock acting to cause mitochondrial depolarization and caspase-9 activation, while TNF-α acts through a receptor-mediated process to activate caspase-8. In some cells, however, TNF-α can also cause mitochondrial depolarization and caspase-9 activation. In the present study, we tested the hypothesis that heat shock at 41 °C and TNF-α induce apoptosis in bovine preimplantation embryos through a caspase-9-dependent mechanism. Treatment of embryos with either heat shock (41 °C) or TNF-α increase

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Austgulen, Rigmor, Lisa Chedwick, Christina Vogt Isaksen, Lars Vatten, and Catherine Craven. "Trophoblast Apoptosis in Human Placenta at Term as Detected by Expression of a Cytokeratin 18 Degradation Product of Caspase." Archives of Pathology & Laboratory Medicine 126, no. 12 (2002): 1480–86. http://dx.doi.org/10.5858/2002-126-1480-taihpa.

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Abstract Context.—Apoptosis occurs in the normal placenta. The monoclonal antibody M30 is directed against a novel epitope of cytokeratin 18 (CK18) that is formed by caspase cleavage early in the apoptotic cascade, and this antibody may therefore be useful for evaluating trophoblast apoptosis. Objective.—We undertook the present study to evaluate the use of monoclonal antibody M30 to assess trophoblast apoptosis in placenta at term. Methods.—We stained paraffin-embedded placental tissues from 15 deliveries at term with M30. We compared positive M30 staining and CK18 staining (as detected by a

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Holmin, Staffan, та Tiit Mathiesen. "Intracerebral administration of interleukin-1β and induction of inflammation, apoptosis, and vasogenic edema". Journal of Neurosurgery 92, № 1 (2000): 108–20. http://dx.doi.org/10.3171/jns.2000.92.1.0108.

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Object. The proinflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor—α (TNFα) are produced intracerebrally in brain disorders such as trauma, ischemia, meningitis, and multiple sclerosis. This investigation was undertaken to analyze the effect of intracerebral administration of IL-1β and TNFα on inflammatory response, cell death, and edema development.Methods. Intracerebral microinjections of these cytokines were administered to rats. The animals were killed 24 or 72 hours after the injections, and their brains were analyzed by using deoxynucleotidyl transferase—mediated deox

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Wang, A. Y., Y. V. Bobryshev, S. M. Cherian, et al. "Expression of Apoptosis-Related Proteins and Structural Features of Cell Death in Explanted Aortocoronary Saphenous Vein Bypass Grafts." Cardiovascular Surgery 9, no. 4 (2001): 319–28. http://dx.doi.org/10.1177/096721090100900403.

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This study aimed to investigate the features of cell death occurring in aortocoronary saphenous vein bypass grafts. Human aortocoronary saphenous vein bypass grafts with angiographic luminal stenosis of >75% were explanted from 14 patients at redo coronary artery bypass grafting. Proteins associated with apoptotic pathways were identified immunohistochemically using antibodies to Bcl-2, Fas, BAX, p53 and CPP32. Cells undergoing DNA fragmentation were identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). DNA synthesis was investigated using the antibody

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Jamnicki-Abegg, Marina, Dorothee Weihrauch, Paul S. Pagel, et al. "Isoflurane Inhibits Cardiac Myocyte Apoptosis during Oxidative and Inflammatory Stress by Activating Akt and Enhancing Bcl-2 Expression." Anesthesiology 103, no. 5 (2005): 1006–14. http://dx.doi.org/10.1097/00000542-200511000-00015.

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Background Volatile anesthetics attenuate apoptosis. The underlying mechanisms remain undefined. The authors tested whether isoflurane reduces apoptosis in cardiomyocytes subjected to oxidative or inflammatory stress by enhancing Akt and B-cell lymphoma-2 (Bcl-2). Methods Adult and neonatal rat ventricular myocytes and atrial HL-1 myocytes were exposed to hypoxia, hydrogen peroxide, or neutrophils with or without isoflurane pretreatment. The authors assessed cell damage and investigated apoptosis using mitochondrial cytochrome c release, caspase activity, and TUNEL assay. They determined expre

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