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1

Kubelková, D., and J. Špak. "Virus diseases of poppy (Papaver somniferum L.) and some other species of the Papaveraceae family – a review." Plant Protection Science 35, No. 1 (1999): 33–36. http://dx.doi.org/10.17221/9671-pps.

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Opium poppy (Papaver somniferum L.) is described in the literature as a natural host of turnip mosaic virus, bean yellow mosaic virus, beet yellows virus and beet mosaic virus, and experimental host of plum pox virus. P. orientale L., a natural host of beet curly top virus, was successfully infected with turnip mosaic virus and cucumber mosaic virus, and P. dubium L. with turnip mosaic virus. P. rhoeas L. is a natural host of turnip mosaic virus, and artificial host of beet yellows, plum pox and cucumber mosaic viruses. P. nudicaule is reported as a natural host of beet curly top, tomato spotted wilt viruses and turnip mosaic, experimentally it was infected with turnip mosaic virus. Eschscholtzia californica Cham. is described as a natural host of aster yellows phytoplasma, and experimental host of bean yellow mosaic virus. In the Czech Republic, only turnip mosaic virus was reliably identified in naturally infected P. somniferum.
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2

Wilson, M. A. "Turnip Mosaic Virus in Alabama." Plant Disease 70, no. 9 (1986): 892c. http://dx.doi.org/10.1094/pd-70-892c.

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3

Shattuck, V. I. "UG1 Turnip Germplasm Possessing Resistance to Turnip Mosaic Virus." HortScience 27, no. 8 (1992): 938–39. http://dx.doi.org/10.21273/hortsci.27.8.938.

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4

Jenner, Keane, Jones, and Walsh. "Serotypic variation in turnip mosaic virus." Plant Pathology 48, no. 1 (1999): 101–8. http://dx.doi.org/10.1046/j.1365-3059.1999.00309.x.

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5

Canady, Mary A., Steven B. Larson, John Day, and Alexander McPherson. "Crystal structure of turnip yellow mosaic virus." Nature Structural & Molecular Biology 3, no. 9 (1996): 771–81. http://dx.doi.org/10.1038/nsb0996-771.

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6

Jenner, C. E., F. Sánchez, K. Tomimura, K. Ohshima, F. Ponz, and J. A. Walsh. "Turnip mosaic virus determinants of virulence for Brassica napus resistance genes." Plant Protection Science 38, SI 1 - 6th Conf EFPP 2002 (2002): S155—S157. http://dx.doi.org/10.17221/10343-pps.

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Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus<br />(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using<br />resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.<br />For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein<br />converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in<br />plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more<br />resistance genes present in another B. napus line.
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7

Farzadfar, Sh, K. Ohshima, R. Pourrahim, A. R. Golnaraghi, S. Sajedi, and A. Ahoonmanesh. "Reservoir Weed Hosts for Turnip mosaic virus in Iran." Plant Disease 89, no. 3 (2005): 339. http://dx.doi.org/10.1094/pd-89-0339c.

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During the summer of 2003, weed samples of Rapistrum rugosum and Sisymbrium loeselii showing severe mosaic, malformation, and stunting were collected from cauliflower fields in Tehran Province of Iran. Using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with specific polyclonal antibodies, the samples were tested for the presence of Beet western yellows virus, Cauliflower mosaic virus, Radish mosaic virus, Turnip crinkle virus, Turnip mosaic virus (TuMV) (DSMZ, Braunschweig, Germany), Cucumber mosaic virus, and Tobacco mosaic virus (Sanofi Diagnostics Pasteur, Marnes-La-Coquette, France). Leaf extracts were used for mechanical inoculation and they produced chlorotic local lesions on Chenopodium amaranticolor, necrotic lesions on leaves and shoot apex necrosis on Nicotiana glutinosa, leaf deformation, mosaic, and stunting on Petunia hybrida, and severe mosaic, distortion, and stunting on Brassica rapa. These symptoms were similar to those that were described previously for TuMV (4). ELISA results showed that the original leaf samples and inoculated indicator plants reacted positively with TuMV antibodies, but not with antibodies for any of the other viruses listed above. Also, reverse transcription-polymerase chain reaction of total RNA extracted from the collected leaf samples using the universal primers for potyviruses (3) resulted in the amplification of two fragments of the expected sizes, approximately 700 and 1,700 bp. TuMV, a member of the genus Potyvirus in the family Potyviridae, is transmitted by aphids in a nonpersistent manner (4). This virus is geographically widespread with a wide host range that can infect 318 species in 156 genera of 43 plant families including, Brassicaceae, Chenopodiaceae, Asteraceae, Cucurbitaceae, and Solanaceae (2,4). R. rugosum and S. loeselii, two annual or biennial plants in the Brassicaceae family, were common and widely distributed in the fields surveyed. The presence of TuMV-infected weed hosts in cauliflower fields may impact disease management strategies. TuMV was first observed on stock plants (Matthiola sp.) in Iran (1). To our knowledge, this is the first report of natural occurrence of TuMV on weed hosts in Iran. References: (1) M. Bahar et al. Iran. J. Plant Pathol. 21:11, 1985. (2) J. R. Edwardson and R. G. Christie. The potyvirus group. Fla. Agric. Exp. Stn. Monogr. Ser. No. 16, 1991. (3) A. Gibbs and A. Mackenzie. J. Virol. Methods 63:9, 1997. (4) J. A. Tomlinson. Turnip mosaic virus. No. 8 in: Descriptions of Plant Viruses. CMI/AAB, Surrey, England, 1970.
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8

Spence, N. J., N. A. Phiri, S. L. Hughes, et al. "Economic impact of Turnip mosaic virus, Cauliflower mosaic virus and Beet mosaic virus in three Kenyan vegetables." Plant Pathology 56, no. 2 (2007): 317–23. http://dx.doi.org/10.1111/j.1365-3059.2006.01498.x.

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9

Nguyen, Ha Anh, Isabelle Jupin, Philippe Decorse, Stephanie Lau-Truong, Souad Ammar, and Nguyet-Thanh Ha-Duong. "Assembly of gold nanoparticles using turnip yellow mosaic virus as an in-solution SERS sensor." RSC Advances 9, no. 55 (2019): 32296–307. http://dx.doi.org/10.1039/c9ra08015e.

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10

FUJISAWA, Ichiro. "Aphid transmission of turnip mosaic virus and cucumber mosaic virus. 2. Transmission from virus mixtures." Japanese Journal of Phytopathology 51, no. 5 (1985): 562–68. http://dx.doi.org/10.3186/jjphytopath.51.562.

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11

Martínez, Fernando, Santiago F. Elena, and José-Antonio Daròs. "Fate of Artificial MicroRNA-Mediated Resistance to Plant Viruses in Mixed Infections." Phytopathology® 103, no. 8 (2013): 870–76. http://dx.doi.org/10.1094/phyto-09-12-0233-r.

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Artificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically downregulate RNAs that contain complementary sequences. Transgenic plants expressing high levels of one or more amiRNAs targeting particular sequences in the genomes of some RNA viruses have shown specific resistance to the corresponding virus. This is the case of the Arabidopsis thaliana transgenic line 12-4 expressing a high level of the amiR159-HC-Pro targeting 21 nucleotides in the Turnip mosaic virus (TuMV) (family Potyviridae) cistron coding for the viral RNA-silencing suppressor HC-Pro that is highly resistant to TuMV infection. In this study, we explored the fate of this resistance when the A. thaliana 12-4 plants are challenged with a second virus in addition to TuMV. The A. thaliana 12-4 plants maintained the resistance to TuMV when this virus was co-inoculated with Tobacco mosaic virus, Tobacco rattle virus (TRV), Cucumber mosaic virus (CMV), Turnip yellow mosaic virus, Cauliflower mosaic virus (CaMV), Lettuce mosaic virus, or Plum pox virus. However, when the plants were preinfected with these viruses, TuMV was able to co-infect 12-4 plants preinfected with TRV, CaMV, and, particularly, CMV. Therefore, preinfection by another virus jeopardizes the amiRNA-mediated resistance to TuMV.
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12

Shin, In-Sun, Doyeong Kim, and Tae-Ju Cho. "Cysteine-Added Mutants of Turnip Yellow Mosaic Virus." Journal of Bacteriology and Virology 48, no. 4 (2018): 137. http://dx.doi.org/10.4167/jbv.2018.48.4.137.

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13

Barnhill, Hannah N., Rachel Reuther, P. Lee Ferguson, Theo Dreher, and Qian Wang. "Turnip Yellow Mosaic Virus as a Chemoaddressable Bionanoparticle." Bioconjugate Chemistry 18, no. 3 (2007): 852–59. http://dx.doi.org/10.1021/bc060391s.

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14

Stobbs, L. W. "Turnip Mosaic Virus Strains in Southern Ontario, Canada." Plant Disease 73, no. 3 (1989): 208. http://dx.doi.org/10.1094/pd-73-0208.

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15

Shields, S. A., and T. M. A. Wilson. "Cell-free Translation of Turnip Mosaic Virus RNA." Journal of General Virology 68, no. 1 (1987): 169–80. http://dx.doi.org/10.1099/0022-1317-68-1-169.

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16

Chivasa, S., E. J. A. Ekpo, and R. G. T. Hicks. "New hosts of Turnip mosaic virus in Zimbabwe." Plant Pathology 51, no. 3 (2002): 386. http://dx.doi.org/10.1046/j.1365-3059.2002.00699.x.

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17

Kaur, Gagandeep, Jinbo He, Ji Xu, et al. "Interfacial Assembly of Turnip Yellow Mosaic Virus Nanoparticles." Langmuir 25, no. 9 (2009): 5168–76. http://dx.doi.org/10.1021/la900167s.

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18

Zhang, Y., R. T. Lartey, S. D. Hartson, T. C. Voss, and U. Melcher. "Limitations to tobacco mosaic virus infection of turnip." Archives of Virology 144, no. 5 (1999): 957–71. http://dx.doi.org/10.1007/s007050050558.

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19

Yoon, Hyun Yee, Kwan Yong Choi, and Byeong Doo Song. "Fluorometric Assay of Turnip Mosaic Virus NIa Protease." Analytical Biochemistry 277, no. 2 (2000): 228–31. http://dx.doi.org/10.1006/abio.1999.4398.

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20

Pink, D. A. C., and D. G. A. Walkey. "Resistance to turnip mosaic virus in white cabbage." Euphytica 51, no. 2 (1990): 101–7. http://dx.doi.org/10.1007/bf00022440.

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21

Martín, África Martín, Héctor Luis Cabrera y Poch, David Martínez Herrera, and Fernando Ponz. "Resistances to Turnip Mosaic Potyvirus in Arabidopsis thaliana." Molecular Plant-Microbe Interactions® 12, no. 11 (1999): 1016–21. http://dx.doi.org/10.1094/mpmi.1999.12.11.1016.

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The responses of a collection of Arabidopsis thaliana eco-types to mechanical inoculation with turnip mosaic poty-virus were assessed. The virus induced characteristic severe symptoms of infection in systemically infected plants. Resistance was found in four ecotypes: Bay-0, Di-0, Er-0, and Or-0. Enzyme-linked immunosorbent assay results of the resistant ecotypes suggested that ecotypes Di-0, Er-0, and Or-0 actually consist of mixed genotypes with resistances acting at different levels in the virus life cycle. Another form of resistance was found in ecotype Bay-0, for which several lines of evidence indicated an interference with viral cell-to-cell movement in the inoculated leaves.
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22

Horsewood, P., M. R. McDermott, L. W. Stobbs, P. L. J. Brais, and B. J. Underdown. "Characterization of a monoclonal antibody to turnip mosaic virus and its use in immunodiagnosis of infection." Phytoprotection 72, no. 2 (2005): 61–68. http://dx.doi.org/10.7202/706004ar.

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Monoclonal antibodies specifie for turnip mosaic virus (TuMV) were produced and used in a double antibody sandwich enzyme immunoassay to detect virus in infected plants. One particular antibody from a hybridoma clone having desirable growth, specificity and antibody production properties was characterized in detail. This antibody was shown by immunocytochemical electron microscopy and immunoblotting to react with a virion coat protein. Conditions providing efficient extraction of virus from leaves were investigated by using the antibody in both capture and detection steps of a sandwich immunoassay. With an extraction buffer System containing multiple detergents, a highly sensitive assay was produced that reliably detected virus in infected plants. This assay is now in routine use for immunodiagnosis of turnip mosaic virus infections.
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23

MASKELL, L. C., A. F. RAYBOULD, J. I. COOPER, M.-L. EDWARDS, and A. J. GRAY. "Effects of turnip mosaic virus and turnip yellow mosaic virus on the survival, growth and reproduction of wild cabbage (Brassica oleracea)." Annals of Applied Biology 135, no. 1 (1999): 401–7. http://dx.doi.org/10.1111/j.1744-7348.1999.tb00867.x.

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24

Kartiningtyas, Kartiningtyas, and Sri Hendrastuti Hidayat. "DETEKSI TURNIP MOSAIC VIRUS PADA JARINGAN BENIH DAN DAUN." Jurnal Hama dan Penyakit Tumbuhan Tropika 6, no. 1 (2006): 32–40. http://dx.doi.org/10.23960/j.hptt.1632-40.

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Detection of Turnip mosaic virus in seed and leaf tissue. The study was conducted to test the seed transmission efficiency of Turnip mosaic virus (TuMV) on caisin (Brassica rapa) and the susceptibility of plant to the virus at different ages. Two detection techniques, ELISA and RT-PCR, were used to determine the more appropriate method for detection of TuMV. Two different sources of seeds involved those from farmer and commercial seeds were collected from West Java and Central Java. TuMV was inoculated on test plants at 2, 4, 6, 8, and 10 weeks after transplanting. Infected plants were confirmed using ELISA and RT-PCR techniques with specific antiserum and primer. TuMV was detected from farmer seeds originated from Ciherang and Cinangneng with percent infection of 15% and 2% , respectively. Plant growth and symptom development were affected by time of infection. In general, TuMV infection caused symptoms, mosaic, malformation, vein clearing, and blister on the leaf. The youngest plants were more susseptble and shown more severe symptoms. Absorbent value of ELISA from infected plants was in the range of 2.1 – 2.4. Spesific DNA band, 800 bp, was amplified from infected plants.
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25

Lilly, S. T., R. S. M. Drummond, M. N. Pearson, and R. M. MacDiarmid. "Identification and Validation of Reference Genes for Normalization of Transcripts from Virus-Infected Arabidopsis thaliana." Molecular Plant-Microbe Interactions® 24, no. 3 (2011): 294–304. http://dx.doi.org/10.1094/mpmi-10-10-0236.

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Real-time quantitative polymerase chain reaction (qPCR) of complementary DNA is now a standard method for studies of gene expression. However, qPCR can identify genuine variation only when transcript quantities are accurately normalized to an appropriate reference. To identify the most reliable reference genes for transcript quantification by qPCR, we describe a systematic evaluation of candidate reference genes of Arabidopsis thaliana ecotype Columbia-0 (Col-0). Twelve genes were selected for transcript stability studies by qPCR of complementary DNA prepared from Arabidopsis leaf tissue infected with one of five plant viruses (Cauliflower mosaic virus, Tobacco mosaic virus, Tomato spotted wilt virus, Turnip mosaic virus, and Turnip yellow mosaic virus). The F-box family protein, elongation factor 1-α, sand family protein, and protodermal factor 2 gene transcripts showed the most stable accumulation, whereas a traditionally used reference gene, Actin8, showed the least stable accumulation as measured by the geNorm algorithm. The data furnish plant virologists with reference genes for normalization of qPCR-derived gene expression in virus-infected Arabidopsis and will be beneficial to the selection and design of primers targeting orthologous genes in other plant species.
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26

Richardson, Lynn G. L. "Hijacking the ER Membrane: Lessons from Turnip mosaic virus." Plant Physiology 179, no. 2 (2019): 367–68. http://dx.doi.org/10.1104/pp.18.01551.

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27

Green, S. K. "Turnip Mosaic Virus Strains in Cruciferous Hosts in Taiwan." Plant Disease 69, no. 1 (1985): 28. http://dx.doi.org/10.1094/pd-69-28.

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28

Stobbs, L. W., and A. Stirling. "Susceptibility of Ontario weed species to turnip mosaic virus." Canadian Journal of Plant Pathology 12, no. 3 (1990): 255–62. http://dx.doi.org/10.1080/07060669009500996.

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29

Walsh, John A., and Carol E. Jenner. "Turnip mosaic virus and the quest for durable resistance." Molecular Plant Pathology 3, no. 5 (2002): 289–300. http://dx.doi.org/10.1046/j.1364-3703.2002.00132.x.

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30

MA, Sevik. "Turnip mosaic virus infecting kale plants in Ordu, Turkey." Phyton 85, no. 1 (2016): 231–35. http://dx.doi.org/10.32604/phyton.2016.85.231.

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31

Matthews, R. E. F., and J. Witz. "Uncoating of turnip yellow mosaic virus RNA in vivo." Virology 144, no. 2 (1985): 318–27. http://dx.doi.org/10.1016/0042-6822(85)90274-0.

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32

Demir, Gizem. "Ülkemiz Turnip Mosaic Virus Bamya İzolatının Tüm Genom Analizi." Cukurova University, Agriculture Faculty 1, no. 36 (2021): 139–48. http://dx.doi.org/10.36846/cjafs.2021.42.

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33

Palukaitis, Peter, and Su Kim. "Resistance to Turnip Mosaic Virus in the Family Brassicaceae." Plant Pathology Journal 37, no. 1 (2021): 1–23. http://dx.doi.org/10.5423/ppj.rw.09.2020.0178.

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34

Gładysz, Karol, and Ewa Hanus-Fajerska. "Evaluation of the infectivity of selected turnip mosaic virus isolates towards white cabbage cultivars." Folia Horticulturae 21, no. 1 (2009): 129–38. http://dx.doi.org/10.2478/fhort-2013-0132.

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Abstract Experiments were carried out to evaluate the reaction of cabbage cultivars to mechanical inoculation with selected isolates of the turnip mosaic virus (TuMV). Simultaneously we aimed for the assessment of TuMV pathogenicity towards cultivars chosen to be transformed in order to obtain the resistance trait. The TuMV-CAR37A and TuMV-CAR39 isolates from horseradish proved to be infective towards ‘Amager’ and ‘Langedijker’ B. oleracea subsp. capitata f. alba. The course of symptom expression was assessed and the results of virus detection in symptomless leaves, using DAS-ELISA, were documented. Both tested cultivars showed a similar level of susceptibility. TuMV-CAR37A and TuMV-CAR39 can be useful in the selection of cabbage lines with resistance to the turnip mosaic virus.
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35

Hao, Yi, Wen Yuan, Chuanxin Ma, et al. "Engineered nanomaterials suppress Turnip mosaic virus infection in tobacco (Nicotiana benthamiana)." Environmental Science: Nano 5, no. 7 (2018): 1685–93. http://dx.doi.org/10.1039/c8en00014j.

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Tobacco (Nicotiana benthamiana) and Turnip mosaic virus (TuMV) were used as a model system to investigate the potential of engineered nanomaterials (ENMs) for promoting crop growth and resistance to viral infection.
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36

Rajamäki, Minna-Liisa, Dehui Xi, Sidona Sikorskaite-Gudziuniene, Jari P. T. Valkonen, and Steven A. Whitham. "Differential Requirement of the Ribosomal Protein S6 and Ribosomal Protein S6 Kinase for Plant-Virus Accumulation and Interaction of S6 Kinase with Potyviral VPg." Molecular Plant-Microbe Interactions® 30, no. 5 (2017): 374–84. http://dx.doi.org/10.1094/mpmi-06-16-0122-r.

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Ribosomal protein S6 (RPS6) is an indispensable plant protein regulated, in part, by ribosomal protein S6 kinase (S6K) which, in turn, is a key regulator of plant responses to stresses and developmental cues. Increased expression of RPS6 was detected in Nicotiana benthamiana during infection by diverse plant viruses. Silencing of the RPS6 and S6K genes in N. benthamiana affected accumulation of Cucumber mosaic virus, Turnip mosaic virus (TuMV), and Potato virus A (PVA) in contrast to Turnip crinkle virus and Tobacco mosaic virus. In addition, the viral genome-linked protein (VPg) of TuMV and PVA interacted with S6K in plant cells, as detected by bimolecular fluorescence complementation assay. The VPg–S6K interaction was detected in cytoplasm, nucleus, and nucleolus, whereas the green fluorescent protein-tagged S6K alone showed cytoplasmic localization only. These results demonstrate that the requirement for RPS6 and S6K differs for diverse plant viruses with different translation initiation strategies and suggest that potyviral VPg–S6K interaction may affect S6K functions in both the cytoplasm and the nucleus.
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37

Farzadfar, S., R. Pourrahim, A. R. Golnaraghi, S. Jalali, and A. Ahoonmanesh. "Occurrence of Radish mosaic virus on Cauliflower and Turnip Crops in Iran." Plant Disease 88, no. 8 (2004): 909. http://dx.doi.org/10.1094/pdis.2004.88.8.909a.

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During the spring and summer of 2003, symptoms of mosaic, mottle, and crinkle were observed in cauliflower (Brassica oleracea) and turnip (Brassica rapa) fields in the Qazvin and Esfahan provinces of Iran, respectively. Leaf extracts of these plants, made infective by mechanical inoculation, caused necrotic local lesions on Chenopodium amaranticolor, chlorotic ring spot on Nicotiana tabacum cv. Samsun, and chlorotic local lesions followed by systemic mosaic on Brassica rapa (1). Using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and specific polyclonal antibodies (As-0120 and PV-0355) that were kindly prepared by S. Winter (DSMZ, Braunschweig, Germany), the samples were tested for the presence of Radish mosaic virus (RaMV) (family Comoviridae, genus Comovirus). ELISA results showed that the original leaf samples and inoculated indicator plants reacted positively to RaMV antibodies. RaMV has been reported in the United States, Japan, and Europe on turnip and other crucifers (1,2). To our knowledge, this is the first report of RaMV occurring in Iran. References: (1) R. N. Campbell. Radish mosaic virus. No. 121 in: Descriptions of Plant Viruses. CMI/AAB, Surrey, England, 1973. (2) D. D. Sutic et al. Handb. Plant Virus Diseases. CRC Press, Boca Raton, FL, 1999.
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38

ŠPAK, J., DARINA KUBELKOVÁ, and E. HNILIČKA. "Seed transmission of turnip yellow mosaic virus in winter turnip and winter oilseed rapes." Annals of Applied Biology 123, no. 1 (1993): 33–35. http://dx.doi.org/10.1111/j.1744-7348.1993.tb04069.x.

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39

de Assis Filho, F. M., and J. L. Sherwood. "Evaluation of Seed Transmission of Turnip yellow mosaic virus and Tobacco mosaic virus in Arabidopsis thaliana." Phytopathology® 90, no. 11 (2000): 1233–38. http://dx.doi.org/10.1094/phyto.2000.90.11.1233.

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The mechanism of virus transmission through seed was studied in Arabidopsis thaliana infected with Turnip yellow mosaic virus (TYMV) and Tobacco mosaic virus (TMV). Serological and biological tests were conducted to identify the route by which the viruses reach the seed and subsequently are located in the seed. Both TYMV and TMV were detected in seed from infected plants, however only TYMV was seed-transmitted. This is the first report of transmission of TYMV in seed of A. thaliana. Estimating virus seed transmission by grow-out tests was more accurate than enzyme-linked immunosorbent assay due to the higher frequency of antigen in the seed coat than in the embryo. Virus in the seed coat did not lead to seedling infection. Thus, embryo invasion is necessary for seed transmission of TYMV in A. thaliana. Crosses between healthy and virus-infected plants indicated that TYMV from either the female or the male parent could invade the seed. Conversely, invasion from maternal tissue was the only route for TMV to invade the seed. Pollination of flowers on healthy A. thaliana with pollen from TYMV-infected plants did not result in systemic infection of healthy plants, despite TYMV being carried by pollen to the seed.
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40

Costa, H., J. A. Ventura, A. S. Jadão, J. A. M. Rezende, and A. P. O. A. Mello. "First Report of Turnip mosaic virus on Watercress in Brazil." Plant Disease 94, no. 8 (2010): 1066. http://dx.doi.org/10.1094/pdis-94-8-1066a.

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Watercress (Nasturtium officinale L.), a member of the family Brassicaceae, is consumed mainly as salad. Medicinal properties have also been attributed to this species. In Brazil, watercress is grown mainly by very small farmers. The crop is primarily seed propagated and growers can harvest several times per year in an established planting. Very few diseases have been reported in this crop worldwide. In Brazil, watercress infection by Cauliflower mosaic virus (CaMV) (3), Cucumber mosaic virus (CMV) (1), and an unidentified potyvirus (2) were previously reported. In January 2009, 80% of watercress plants, cv. Gigante Redondo, exhibiting severe mosaic, leaf size reduction, and plant stunting were observed in a crop in Marechal Floriano Municipality, State of Espírito Santo, Brazil. Preliminary leaf dip analysis by transmission electron microscopy revealed the presence of potyvirus-like particles. Sap from five infected plants reacted in plate-trapped antigen (PTA)-ELISA with polyclonal antiserum against Turnip mosaic virus (TuMV), but not with antiserum against CMV. Both antisera were produced in the Plant Virology Laboratory, ESALQ/USP. Mechanically inoculated watercress plants developed similar systemic mosaic symptoms. The virus was also transmitted to Nicotiana benthamiana, which exhibited severe mosaic and stunting. The presence of TuMV on these inoculated plants was confirmed by PTA-ELISA and reverse transcription (RT)-PCR. Total RNA extracted from infected and healthy watercress and infected N. benthamiana was analyzed by RT-PCR using specific pairs of primers flanking the coat protein gene of TuMV. Degenerated anti-sense (5′-t/caacccctt/gaacgcca/cagt/ca-3′) and sense (5′-gcaggtgaa/gacg/acttgat/ca/gc-3′) primers were designed after analysis to an alignment of the nucleotide sequences for five isolates of TuMV available in the GenBank (Accession Nos. NC_002509, D10927, EU680574, AB362513, and D88614). One fragment of 838 bp was amplified from samples in the infected plants, but not in the healthy controls. Two amplicons were purified and directly sequenced in both directions. Comparisons of the 731-bp consensus nucleotide sequence (Accession No. HM008961) to several other isolates of TuMV revealed 94 to 95% identity in the coat protein region. To our knowledge, this is the first report of TuMV in watercress in Brazil. Management of the disease should include propagation by seeds instead of vegetative parts of the plants and rouging of diseased plants to prevent mechanical transmission during successive harvestings. References: (1) A. J. Boari et al. Fitopatol. Bras. 25:438, 2000. (2) A. J. Boari et al. Fitopatol. Bras. 27:S200, 2002. (3) M. L. R. Z. C. Lima et al. Fitopatol. Bras. 9:403, 1984.
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41

Špak, J., and D. Kubelková. "Field resistance of six cultivars of winter oilseed rape against Turnip yellow mosaic virus." Plant Protection Science 38, No. 2 (2012): 73–75. http://dx.doi.org/10.17221/4852-pps.

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The resistance of six cultivars of winter oilseed rape (SL 509, SL 507, Darmor, Solida, Jet Neuf, Silesia) against Turnip yellow mosaic virus was studied. The number of over-wintering plants and plants with symptoms of TYMV infection were monitored. All plants were tested by the double diffusion test in agar and by DAS-ELISA to prove infection. ELISA was the most sensitive method, revealing 32–76% of latent virus infections in individual cultivars. The results clearly illustrated that monitoring of plant symptoms is not sufficient to prove TYMV infection in the field and that sensitive, large scale methods like ELISA must be employed to obtain reliable data.
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42

SANO, Yositaka, and Makoto KOJIMA. "Increase in cucumber mosaic virus concentration in Japanese radish plants co-infected with turnip mosaic virus." Japanese Journal of Phytopathology 55, no. 3 (1989): 296–302. http://dx.doi.org/10.3186/jjphytopath.55.296.

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43

Gungoosingh, A., S. P. Beni Madhu, and D. Dumur. "First Report of Turnip mosaic virus in Watercress in Mauritius." Plant Disease 85, no. 8 (2001): 919. http://dx.doi.org/10.1094/pdis.2001.85.8.919b.

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In July 1999, leaf mosaic and distortions were observed on watercress in the region of Camp de Masque, in the eastern part of Mauritius. Electron microscopy of crude sap preparations revealed the presence of 720 nm, flexuous filamentous particles. Virus detection by reverse-transcription polymerase chain reaction (RT-PCR) and virus sequencing by A. Mackenzie (Research School of Biological Sciences, Australian National University) confirmed the identity of the causal pathogen as Turnip mosaic virus (TuMV) in November 1999. A survey was initiated in January 2000, covering the 44 major watercress ponds across the island. Two hundred seventy-five samples (231 symptomatic; 44 symptomless) were collected from 22 localities. TuMV was detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using commercial antisera (Agdia Inc.). Plant extracts were prepared by cutting approximately 20 g of leaf samples into small pieces, from which 1.0 to 1.5 g were used in the evaluation. Eighty-one percent of symptomatic samples (187 out of 231) were TuMV positive; all of the symptomless samples were TuMV negative. Symptoms on infected watercress included leaf mosaic, mottling, distortions, general yellowing, and plant stunting. TuMV has since been detected on all three commonly grown watercress varieties in Mauritius: Brède Doux, Brède Blanc, and Constance. Under local conditions, TuMV affects the quality and thus the commercial value of the crop. Additional hosts of TuMV among local brassicas are also being determined, and to date the virus has been detected in turnip (Brassica campestris spp. rapa), pak choi (Brassica campestris spp. chinensis), and petsai (Brassica campestris spp. pekinensis).
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44

Yang, Hui, Shaodong Wang, Dehui Xi, et al. "Interaction between Cucumber mosaic virus and Turnip crinkle virus in Arabidopsis thaliana." Journal of Phytopathology 158, no. 11-12 (2010): 833–36. http://dx.doi.org/10.1111/j.1439-0434.2010.01694.x.

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45

Chen, Y. K., Y. S. Chang, and H. J. Bau. "First Report of Turnip ringspot virus in Field Mustard (Brassica chinensis) in Taiwan." Plant Disease 95, no. 8 (2011): 1036. http://dx.doi.org/10.1094/pdis-03-11-0224.

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Crucifer crops (Brassica spp.) are important winter vegetables in Taiwan. Five viruses, including Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV), Radish mosaic virus (RaMV), Beet western yellows virus (BWYV), and Cauliflower mosaic virus (CaMV), have been detected in a range of domestic-grown crucifers during past decades (1). Field mustard plants (Brassica chinensis) showing mosaic in the leaves were collected in the ChiaYi area in December 2007. Spherical virus-like particles, approximately 30 nm in diameter, were readily observed in crude sap of symptomatic plants. Tests by ELISA failed to detect any of the aforementioned viruses. A spherical agent was isolated through mechanical inoculation onto Chenopodium quinoa, and a virus culture was established and inoculated mechanically back to the original host as well as other crucifers. Systemic mosaic appeared on inoculated B. campestris, B. chinensis, and B. juncea, whereas ringspots appeared on inoculated leaves of B. oleracea. Total RNA was extracted from symptomatic leaves and used for reverse transcription (RT)-PCR amplification using degenerate primers for comoviruses (2). Other successive fragments of RNAs 1 and 2 were amplified by specific or degenerate primers designed on the basis of sequences of published Turnip ringspot virus (TuRSV). The RNA 1 (GenBank Accession No. GU968732) and RNA 2 (No. GU968731) of the isolated virus consisted of 6,076 and 3,960 nucleotides, respectively. The number of nucleotides and the arrangement of open reading frames on both RNA 1 and RNA 2 were similar to those of comoviruses. Sequence analysis revealed that the nucleotide sequences of RNA 1 and RNA 2 shared 54.2 to 82.5% and 50.2 to 79.3% similarities, respectively, to those of comoviruses and were most similar to Turnip ringspot virus. The deduced peptides of large and small coat proteins (LCP and SCP) contain 375 amino acids (41.2 kDa) and 251 amino acids (28.5 kDa), respectively. The deduced amino acid sequences of RNA-dependent RNA polymerase (RdRp), LCP, and SCP share 92.0 to 94.5%, 93.1 to 93.3% and 87.3 to 89.6% similarity, respectively, to those of published TuRSV isolates, i.e., -B (GenBank Accession No. GQ222382), -M12 (No. FJ516746), and -Toledo (No. FJ712027) indicating that the newly isolated virus from field mustard in Taiwan is an isolate of TuRSV, hence TuRSV-TW. Comparison of LCP and SCP between current TuRSV-TW and Radish mosaic virus (RaMV; GenBank Accession No. AB295644) showed 74% similarity, which is below the species demarcation level of 75% (3), indicating its discrimination from RaMV. To our knowledge, this is the first report of the occurrence of TuRSV in Taiwan and in the subtropics. References: (1) T. H. Chen et al. Plant Pathol. Bull. 9:39, 2000. (2) V. Maliogka et al. J. Phytopathol. 152:404, 2004. (3) K. Petrzik and I. Koloniuk. Virus Genes 40:290, 2010.
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46

Milosevic, Dragana, Maja Ignjatov, Zorica Nikolic, et al. "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia." Pesticidi i fitomedicina 31, no. 1-2 (2016): 37–44. http://dx.doi.org/10.2298/pif1602037m.

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A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.
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47

Eiras, Marcelo, Alexandre L. R. Chaves, Addolorata Colariccio, and César M. Chagas. "First report of Turnip mosaic virus in horseradish in Brazil." Fitopatologia Brasileira 32, no. 2 (2007): 165. http://dx.doi.org/10.1590/s0100-41582007000200013.

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48

Doucet, R., V. I. Shattuck, and L. W. Stobbs. "Rutabaga Germplasm TuMV-R Possessing Resistance to Turnip Mosaic Virus." HortScience 25, no. 5 (1990): 583–84. http://dx.doi.org/10.21273/hortsci.25.5.583.

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49

Shin, Hyun-Il, In-Cheol Kim, and Tae-Ju Cho. "Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA." BMB Reports 41, no. 10 (2008): 739–44. http://dx.doi.org/10.5483/bmbrep.2008.41.10.739.

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50

Larson, Steven B., Robert W. Lucas, Aaron Greenwood, and Alexander McPherson. "The RNA of turnip yellow mosaic virus exhibits icosahedral order." Virology 334, no. 2 (2005): 245–54. http://dx.doi.org/10.1016/j.virol.2005.01.036.

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