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1

Roux, Huguette, C. Paass, Konan Djaha, G. Agneroh-Eboi, and A. Aka. "Triangle de TWEED. Équilibre de la denture et des tissus mous. Étude sur une population de jeunes Ivoiriens." Revue d'Orthopédie Dento-Faciale 27, no. 3 (1993): 297–304. http://dx.doi.org/10.1051/odf/1993030.

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2

Meehan, J. T., R. C. Cutlip, and H. D. Lehmkuhl. "Evaluation of Ethylenediaminetetraacetic Acid, Tetrasodium Salt Dihydrate (EDTA)-Tween 20 Treatment versus Protease Digestion of Formalin-fixed Tissue Sections for Detection of Bovine Respiratory Syncytial Virus Antigen in Infected Ovine Lung." Veterinary Pathology 26, no. 4 (1989): 322–25. http://dx.doi.org/10.1177/030098588902600406.

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The efficacy of protease and ethylenediaminetetraacetic acid, tetrasodium salt dihydrate (EDTA)-Tween 20 in unmasking bovine respiratory syncytial virus (BRSV) antigens in formalin-fixed lung tissue was compared using avidin-biotin immunoperoxidase procedure. Tissues were taken from experimentally infected lambs. BRSV antigen stained in both techniques. Treatment with EDTA-Tween 20 resulted in more intense staining of BRSV infected cells, more uniform cytoplasmic staining, less non-specific background, and superior cellular detail in comparison to protease digestion.
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3

Karimifar, Haddis, Mina Allameh, Arsalan Soleimanian, Asghar Zeyni-Esfahani, and Abdolamir Allameh. "Augmentation of lipid peroxidation and tissue injuries in rats exposed by sub-acute inhalation to nano-silver particles containing tween-20." Interdisciplinary Toxicology 13, no. 4 (2020): 121–27. https://doi.org/10.2478/intox-2020-0017.

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Abstract Nanosilver particles (NSPs) used by customers may contain detergents/solvents. However, in most of the toxicological studies, nanosilvers are prepared in water and very little is known about the interaction of these agents with NSPs in causing adverse effects. The aim of this study was to investigate the interaction of NSPs and tween-20 in causing tissue injuries after inhalation. In this experiment, 16 rats were divided into four groups (n=4/group) as follows. Controls, exposed to water vapors; Tween-treated exposed to tween-20 alone (0.01% V/V); NSP-tween group exposed to 1000 ppm c
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4

Lust, Cody A. C., Xinjie Lin, Erin M. Rock, Cheryl L. Limebeer, Linda A. Parker, and David W. L. Ma. "Short communication: Tissue distribution of major cannabinoids following intraperitoneal injection in male rats." PLOS ONE 17, no. 1 (2022): e0262633. http://dx.doi.org/10.1371/journal.pone.0262633.

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Currently, peripheral tissue distribution of cannabinoids after treatment is poorly understood. This pilot study sought to examine the early tissue distribution of major cannabinoids 30 minutes following an intraperitoneal injection of vehicle (1:9 Tween 80/SAL), and doses of THC (1 mg/kg) and CBD (5 mg/kg) that are feasible for human consumption in serum, adipose, brain, lung, liver, jejunum, and muscle of male Sprague-Dawley rats. The jejunum and adipose were most enriched in THC. Similarly, CBD was enriched in the jejunum and adipose but also the liver. In contrast, the brain had the lowest
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5

Lownds, N. K., and M. J. Bukovac. "Surfactant-induced Ethylene Production by Leaf Tissue." Journal of the American Society for Horticultural Science 114, no. 3 (1989): 449–54. http://dx.doi.org/10.21273/jashs.114.3.449.

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Abstract Ethylene evolution induced by nonionic (Triton X-100, Triton X-405, Tween 20, Ortho X-77 and Regulaid), anionic (Aerosol OT and Dupanol ME), and cationic (Arquad C-50 and Arquad 2C-75) surfactants was characterized using cowpea [Vigna unguiculata (L.) Walp. supsb. unguiculata ‘Dixielee’] seedlings. Representative surfactants of each ionogenic class induced ethylene evolution. Time course studies revealed an increased rate of ethylene evolution during the first 6 to 12 hr after treatment, followed by a slow decrease for the next 12 to 36 hr, and a return to control levels within 48 hr.
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6

Lu, Huihui, Francesco Floris, Marc Rensing, and Stefan Andersson-Engels. "Fluorescence Spectroscopy Study of Protoporphyrin IX in Optical Tissue Simulating Liquid Phantoms." Materials 13, no. 9 (2020): 2105. http://dx.doi.org/10.3390/ma13092105.

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Fluorescence spectroscopy has been extensively investigated for disease diagnosis. In this framework, optical tissue phantoms are widely used for validating the biomedical device system in a laboratory environment outside of clinical procedures. Moreover, it is fundamental to consider that there are several scattering components and chromophores inside biological tissues and the interplay between scattering and absorption may result in a distortion of the emitted fluorescent signal. In this work, the photophysical behaviour of a set of liquid, tissue-like phantoms containing different composit
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7

Morita, Tetsuo, and Tatsuo Shimada. "Surface morphology of Purkinje cells and myocardial cells following chemical digestion." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 420–21. http://dx.doi.org/10.1017/s0424820100159643.

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From light and transmission electron microscopic studies, it has been long known that Purkinje cells of mammalian hearts have morphological characteristics different from ordinary myocardial cells. In the present study, not only Purkinje cells and myocardial cells but also connective tissue sheaths surrounding these cells were investigated by combined scanning electron microscopy(SEM) and chemical digestion.The moderator band of adult sheep heart was used because it possessed both Purkinje cells and myocardial cells (Fig.1). Tissue blocks were immersed in Karnovsky’s fixative for 3hr or longer
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8

Gagné, François. "Isolation and Quantification of Polystyrene Nanoplastics in Tissues by Low Pressure Size Exclusion Chromatography." Journal of Xenobiotics 12, no. 2 (2022): 109–21. http://dx.doi.org/10.3390/jox12020010.

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Ecotoxicity investigations of plastic nanoparticles (NPs) should pay more attention to their ability to pass barriers, accumulate, and initiate toxicity in cells. The purpose of this study was to develop a simple size exclusion chromatography (SEC) methodology to measure plastic NPs in biological tissues. A SEC column was prepared using a high-resolution gel for large macromolecules to separate plastic NPs from the protein/lipid pools in tissues. It was necessary to prepare the samples in high salt and non-ionic detergent (0.5 M NaCl and 0.2% Tween-20) and apply 0.2% Tween-20 containing 14 mM
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9

Kshitiza, Pathak, and Pathak Akshay. "CROSS-SECTIONAL ASSESSMENT OF ASSOCIATION BETWEEN GRADE OF AS-THI SARATA AND INCIDENCE OF DENTAL CARIES." International Ayurvedic Medical Journal 8, no. 8 (2020): 4032–35. http://dx.doi.org/10.46607/iamj0108082020.

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Dashavidha Karanadi Pariksha (Ten-fold examination) elaborated in Charaka Samhita imparts complete knowledge of patient’s condition by means of specific investigations. It is done for knowledge of lifespan, degree of strength of body and disease and exact treatment perspective. Accordingly, a patient should be examined in respect of Dhatu Sarata (excellence of body tissues) i.e. as per the best qualities of Dhatu (body tissues). Drudh Danta (strongness of teeth) has been described as a characteristic of Asthi Sarata. Thus, an observational cross-sectional study was planned to assess the probab
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10

Bytov, Maksim V., Irina M. Petrova, Sergey L. Khatsko, Olga V. Sokolova, and Irina A. Shkuratova. "Protocol refinement for quenching autofluorescence of red blood cells in FFPE sections of organ samples from cattle, pigs and chickens." BIO Web of Conferences 108 (2024): 01034. http://dx.doi.org/10.1051/bioconf/202410801034.

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One of the most common problem that researchers encounter when using fluorescence to visualize immunohistochemistry is the autofluorescence of the studied organ tissue sections and cell cultures. Autofluorescence quenching is necessary for a wide variety of organs and tissues, as well as for different methods of fixation and histochemical processing of sections. In addition to autofluorescence quenching, it is necessary to take into account the need for histological readability of tissue sections when using counterstains afterwards. Such protocol refinement for fluorescent immunohistochemistry
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11

Xing, Zhe, Jiazheng Cai, Yang Sun, et al. "Altered Surface Hydrophilicity on Copolymer Scaffolds Stimulate the Osteogenic Differentiation of Human Mesenchymal Stem Cells." Polymers 12, no. 7 (2020): 1453. http://dx.doi.org/10.3390/polym12071453.

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Background: Recent studies have suggested that both poly(l-lactide-co-1,5-dioxepan-2-one) (or poly(LLA-co-DXO)) and poly(l-lactide-co-ε-caprolactone) (or poly(LLA-co-CL)) porous scaffolds are good candidates for use as biodegradable scaffold materials in the field of tissue engineering; meanwhile, their surface properties, such as hydrophilicity, need to be further improved. Methods: We applied several different concentrations of the surfactant Tween 80 to tune the hydrophilicity of both materials. Moreover, the modification was applied not only in the form of solid scaffold as a film but also
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12

Chuanlai, X., P. Cifang, H. Kai, J. Zhengyu, and W. Wukang. "Quantitative analysis of chloramphenicol residues in shrimp muscle tissues by Chemiluminescent enzyme immunoassay." Czech Journal of Food Sciences 23, No. 6 (2011): 251–56. http://dx.doi.org/10.17221/3399-cjfs.

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A competitive indirect chemiluminescent enzyme immunoassay (ic-CLEIA) has been developed for the determination of chloramphenicol (CAP) residues in shrimp. After the optimisation of four physico-chemical parameters, i.e. incubation time, concentration of Tween-20, concentration of PBS and its pH, the method developed gave a limit of detection of 0.01 ng/ml and a detection range from 0.03 ng/ml to 23.7 ng/ml, with an ED<sub>50</sub> of 0.47 ng/ml. The developed method has been validated on spiked shrimp samples in terms of precision (intra- and interassay coefficient variations of l
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13

Kömüves, László G. "The presence of active, mature goblet cells in the small intestine of newborn miniature piglets." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 30–31. http://dx.doi.org/10.1017/s0424820100145996.

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Goblet cells in the intestine produce intestinal mucus, which acts as a protective barrier. Histochemical studies in 1-wk-old piglets have shown that goblet cells were fully mature, and contained both neutral and acidic mucopolysaccharides. There is no information, however, about the secretory activity of goblet cells in newborn piglets. We present evidence that goblet cells in newborn, nonsuckled miniature piglets actively synthesize and secrete glycoproteins, as detected by wheat germ agglutiningold conjugates (WGA-AU15) in LR White-embedded tissues. Details of the surgical procedures, tissu
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14

McClellan, Phillip, Robin Jacquet, Qing Yu, and William J. Landis. "A Method for the Immunohistochemical Identification and Localization of Osterix in Periosteum-Wrapped Constructs for Tissue Engineering of Bone." Journal of Histochemistry & Cytochemistry 65, no. 7 (2017): 407–20. http://dx.doi.org/10.1369/0022155417705300.

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A novel immunohistochemistry (IHC) approach has been developed to label and localize osterix, a bone-specific transcription factor, within formalin-fixed, paraffin-embedded, tissue-engineered constructs uniquely containing synthetic polymers and human periosteal tissue. Generally, such specimens consisting in part of polymeric materials and mineral are particularly difficult for IHC identification of proteins. Samples here were fabricated from human periosteum, electrospun poly-l-lactic acid (PLLA) nanofibers, and polycaprolactone/poly-l-lactic acid (PCL/PLLA, 75/25) scaffolds and harvested fo
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15

Korolev, Dmitry, Michael Shumilo, Galina Shulmeyster, et al. "Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles." Nanomaterials 11, no. 6 (2021): 1484. http://dx.doi.org/10.3390/nano11061484.

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In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (&g
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16

Warmuz, Justyna, Beata Kawala, and Liwia Minch. "Complete analysis of space in the dental arch used in the Tweed-Merrifield technique as a key to prepare a maximally individual plan of orthodontic treatment." Forum Ortodontyczne 13, no. 4 (2017): 283–90. http://dx.doi.org/10.5604/01.3001.0010.8773.

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The Tweed-Merrifield technique is based on a simple and detailed diagnosis and therapy considering a shape of the dental arch with preservation or improvement of facial features.<b> Aim</b>. The aim of the work was to present a reliable and structured method to analyse diagnostic models used in the Tweed-Merrifield technique. <b>Material and methods</b>. Based on a review of available literature the paper presents rules to assess an amount of space in the arch for tooth movements used in the Tweed-Merrifield technique. The analysis of models in this technique is based o
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17

Gotfredsen, E., J. Kragskov, and A. Wenzel. "Development of a system for craniofacial analysis from monitor-displayed digital images." Dentomaxillofacial Radiology 28, no. 2 (1999): 123–26. http://dx.doi.org/10.1038/sj/dmfr/4600420.

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OBJECTIVES To develop a program for clinical craniofacial analysis directly on monitor-displayed digital images. METHODS The program was developed in VisualBasic (Microsoft, Redwood WA, USA), using Access (Microsoft) database, for landmark identification analysis and data storage. It runs on Windows95 (Microsoft). RESULTS The following established cephalometric analyses were included in the program: Bjork, Down, Frontal, Jarabak, Occlusogram, Rickett and Tweed. In addition, an orthodontist can simply define his own analyses. If he wants to identify a particular landmark, he clicks on its name
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18

Asilian, Ebrahim, Reza Ghasemi-Fasaei, Abdolmajid Ronaghi, Mozhgan Sepehri, and Ali Niazi. "Chemical- and microbial-enhanced phytoremediation of cadmium-contaminated calcareous soil by maize." Toxicology and Industrial Health 35, no. 5 (2019): 378–86. http://dx.doi.org/10.1177/0748233719842752.

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Phytoremediation is an appropriate technology used to remove pollutants from environment components. A greenhouse trial was conducted to test the hypothesis that application of surfactant levels and inoculation with Pseudomonas fluorescens bacterium and/or Piriformospora indica fungus enhances the phytoremediation of cadmium (Cd). Maize seeds were sown in Cd-polluted soil, and after 2 months Cd status in plant tissues and Cd phytoremediation criteria was determined. Results showed that application of surfactant increased root and shoot dry weight. Mean Cd uptake in roots and shoots increased f
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19

Aldossary, Sara Abdulraman. "Sesamol counter act toxicity of arsenic on testicular tissues." Journal of medical pharmaceutical and allied sciences 11, no. 5 (2022): 5259–63. http://dx.doi.org/10.55522/jmpas.v11i5.3794.

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The main aim of the study includes analyzing the protective effects of sesamol of testicular in arsenic-induced toxicity. Primarily there have been different treatments and sample preparation methods that includethe mixing of drugs seasonal and arsenic, as they were immersed into the aqueous solution of tween 80 and further arsenic stabilisation was done using gum of 0.2%. A total of four groups, each group having 8 rats selected. Testicular catalase is decreased in arsenic treated rats whereas testicular (Glutathione synthetase) GSH, testicular (nitric oxide) NO and testicular malondialdehyde
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20

Keddari, Soumia, Iyas Aldib, and Ali Riazi. "In vivo stimulatory effects of wheat bran on intestinal microbial ecosystem of mice." South Asian Journal of Experimental Biology 4, no. 1 (2014): 24–32. http://dx.doi.org/10.38150/sajeb.4(1).p24-32.

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The prebiotic effect in vivo of wheat bran on growth and colonization of both strains bifidobacteria (Bifidobacterium animalis subsp lactis Bb12 and B. bifi-dum Bb 443) and two lactic strains (Lactobacillus acidophilus LbA - CEC-T4529; L.rhamnosus LBRE - LSAS) was investigated. Conventional Swiss mice received a daily dose of orogastric monocultures of different strains tested for 4 weeks. These mice were fed a diet supplemented with or without wheat bran by weight. Changes in weight, implantation of strains and modifi-cation of microflora were monitored throughout the study. At the end of the
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21

Smith, Bradford J., Lennart K. A. Lundblad, Michaela Kollisch-Singule, et al. "Predicting the response of the injured lung to the mechanical breath profile." Journal of Applied Physiology 118, no. 7 (2015): 932–40. http://dx.doi.org/10.1152/japplphysiol.00902.2014.

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Mechanical ventilation is a crucial component of the supportive care provided to patients with acute respiratory distress syndrome. Current practice stipulates the use of a low tidal volume (Vt) of 6 ml/kg ideal body weight, the presumptive notion being that this limits overdistension of the tissues and thus reduces volutrauma. We have recently found, however, that airway pressure release ventilation (APRV) is efficacious at preventing ventilator-induced lung injury, yet APRV has a very different mechanical breath profile compared with conventional low-Vt ventilation. To gain insight into the
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22

Dmitriev, М. О. "Determination of individual teleroentgenographic characteristics of the face profile in ukrainian young men and girls with orthognathic bite." Biomedical and Biosocial Anthropology, no. 32 (September 20, 2018): 28–34. http://dx.doi.org/10.31393/bba32-2018-04.

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Modern dentistry requires the definition of individualized values of teleroentgenographic indicators. To solve such problems, methods of regression and correlation analysis are increasingly used, which help to establish not only the existence of various relationships between the anatomical structures of the head and the parameters of the dento-jaw system, but also allow more accurately predict the change in the contour of soft facial tissue in response to orthodontic treatment. The purpose of the study is to develop mathematical models for the determination of individual teleroentgenographic c
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23

Brandl, Maria T., and Steven Huynh. "Effect of the Surfactant Tween 80 on the Detachment and Dispersal of Salmonella enterica Serovar Thompson Single Cells and Aggregates from Cilantro Leaves as Revealed by Image Analysis." Applied and Environmental Microbiology 80, no. 16 (2014): 5037–42. http://dx.doi.org/10.1128/aem.00795-14.

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ABSTRACTSalmonella entericahas the ability to form biofilms and large aggregates on produce surfaces, including on cilantro leaves. Aggregates ofS. entericaserovar Thompson that remained attached to cilantro leaves after rigorous washing and that were present free or bound to dislodged leaf tissue in the wash suspension were observed by confocal microscopy. Measurement ofS.Thompson population sizes in the leaf washes by plate counts failed to show an effect of 0.05% Tween 80 on the removal of the pathogen from cilantro leaves 2 and 6 days after inoculation. On the contrary, digital image analy
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24

Armadany, Fery Indradewi, Laode Muhammad Fitrawan, Fitri Rahmadani Saputri, Vica Aspadiah, and Henny Kasmawati. "Combination of Gotu Kola (Centella asiatica (L.)) Ethyl Acetate Extract and Virgin Coconut Oil (VCO) as Burn Healing." Journal Borneo 3, no. 2 (2023): 95–105. http://dx.doi.org/10.57174/j.born.v3i2.89.

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Burns result from exposure to heat sources like fire, chemicals, or radiation and can lead to tissue loss or damage. Traditional healing of burns often involves using herbal remedies like gotu kola and coconut, typically oil extracts. This study aims to identify the healing effects of burns by administering a combination of ethyl acetate extract of gotu kola herb and virgin coconut oil (VCO) to mice. The mice were divided into 7 groups, namely negative control (K(-)) was given tween 20; positive control (K(+)): burn topical medicine on the market, P1: 10% Ethylacetate extract in tween 20; P2:
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25

Zaki Hammodi, Hiba, and Nada Naji Al-Shawi. "Protective Effect of Daidzein on Ifosfamide-Induced Neurotoxicity Via Improving Some Selected Oxidative Stress Parameters in Male Rats." Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) 32, Suppl. (2023): 53–60. http://dx.doi.org/10.31351/vol32isssuppl.pp53-60.

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In this study, the possible protective effects of daidzein on ifosfamide-induced neurotoxicity in male rats were examined by the determination of changes in selected oxidant–antioxidant markers of male rats’ brain tissue.
 Twenty-eight (28) apparently-healthy Wistar male rats weighing (120-150gm) allocated into 4 groups (n=7) were used in this study. Rats orally-administered 1% tween 20 dissolved in distilled water/Control (Group I); rats were orally-administered daidzein suspension (100mg/kg) for 7 days (Group II); rats intraperitoneally-injected with a single dose of ifosfamide (500 mg/
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26

Roacho-Pérez, Jorge A., Fernando G. Ruiz-Hernandez, Christian Chapa-Gonzalez, et al. "Magnetite Nanoparticles Coated with PEG 3350-Tween 80: In Vitro Characterization Using Primary Cell Cultures." Polymers 12, no. 2 (2020): 300. http://dx.doi.org/10.3390/polym12020300.

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Some medical applications of magnetic nanoparticles require direct contact with healthy tissues and blood. If nanoparticles are not designed properly, they can cause several problems, such as cytotoxicity or hemolysis. A strategy for improvement the biological proprieties of magnetic nanoparticles is their functionalization with biocompatible polymers and nonionic surfactants. In this study we compared bare magnetite nanoparticles against magnetite nanoparticles coated with a combination of polyethylene glycol 3350 (PEG 3350) and polysorbate 80 (Tween 80). Physical characteristics of nanoparti
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Zukancic, Danijela, Estelle J. A. Suys, Emily H. Pilkington, Azizah Algarni, Hareth Al-Wassiti, and Nghia P. Truong. "The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles." Pharmaceutics 12, no. 11 (2020): 1068. http://dx.doi.org/10.3390/pharmaceutics12111068.

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Targeted delivery of nucleic acids to lymph nodes is critical for the development of effective vaccines and immunotherapies. However, it remains challenging to achieve selective lymph node delivery. Current gene delivery systems target mainly to the liver and typically exhibit off-target transfection at various tissues. Here we report novel lipid nanoparticles (LNPs) that can deliver plasmid DNA (pDNA) to a draining lymph node, thereby significantly enhancing transfection at this target organ, and substantially reducing gene expression at the intramuscular injection site (muscle). In particula
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28

Jabbar, Ahmed A. J., Zaenah Zuhair Alamri, Mahmood Ameen Abdulla, Ahmed S. AlRashdi, Soran Kayfi Najmaldin, and Mustafa AbdulMonam Zainel. "Sinapic Acid Attenuate Liver Injury by Modulating Antioxidant Activity and Inflammatory Cytokines in Thioacetamide-Induced Liver Cirrhosis in Rats." Biomedicines 11, no. 5 (2023): 1447. http://dx.doi.org/10.3390/biomedicines11051447.

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Sinapic acid (SA) is a natural pharmacological active compound found in berries, nuts, and cereals. The current study aimed to investigate the protective effects of SA against thioacetamide (TAA) fibrosis in rats by histopathological and immunohistochemical assays. The albino rats (30) were randomly divided into five groups (G). G1 was injected with distilled water 3 times/week and fed orally daily with 10% Tween 20 for two months. G2–5 were injected with 200 mg/kg TAA three times weekly for two months and fed with 10% Tween 20, 50 mg/kg silymarin, 20, and 40 mg/kg of SA daily for 2 months, re
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29

Marefati, Narges, Farimah Beheshti, Akbar Anaeigoudari, et al. "The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats." Journal of Cardiovascular and Thoracic Research 15, no. 2 (2023): 106–15. http://dx.doi.org/10.34172/jcvtr.2023.31719.

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Introduction: Inflammation and oxidative stress are contributed to cardiovascular diseases. Vitamin D (Vit D) has antioxidant and anti-inflammatory properties. In the current research, the effect of Vit D on cardiac fibrosis and inflammation, and oxidative stress indicators in cardiovascular tissues was studied in lipopolysaccharides(LPS) injected rats. Methods: Rats were distributed into 5 groups and were treated for 2 weeks. Control: received vehicle(saline supplemented with tween-80) instead of Vit D and saline instead of LPS, LPS: treated by 1 mg/kg of LPS and was given vehicle instead of
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Rancan, Fiorenza, Jana Jurisch, Cemre Günday, et al. "Screening of Surfactants for Improved Delivery of Antimicrobials and Poly-Lactic-co-Glycolic Acid Particles in Wound Tissue." Pharmaceutics 13, no. 7 (2021): 1093. http://dx.doi.org/10.3390/pharmaceutics13071093.

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Topical wound management is often a challenge due to the poor penetration of antimicrobials in wound tissue and across the biofilm matrix where bacteria are embedded. Surfactants have been used for decades to improve the stability of formulations, increase drug solubility, and enhance penetration. In this study, we screened different detergents with respect to their cytotoxicity and their ability to improve the penetration of poly-lactic-co-glycolic acid (PLGA) particles in wound tissue. Among the tested surfactants, Kolliphor SLS and Tween 80 increased the penetration of PLGA particles and ha
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31

Skaptsov, Mikhail, Maxim Kutsev, Maxim Filipenko, Evgeniy Khrapov, and Harue Shinoyama. "Effect of Modified Heptamethyltrisiloxane on the Efficiency of Agrobacterium-Mediated Transformation and Expression of Recombinant Structure in Plant Cell and Tissue Culture." Key Engineering Materials 683 (February 2016): 506–10. http://dx.doi.org/10.4028/www.scientific.net/kem.683.506.

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Detergents represent a unique class of chemical compounds. They can alter surface and interphase bonds, and form micellar systems. These detergent properties allow to alter wettability of surfaces, stabilize or destabilize dispersed systems, and modify the properties of liquid phases. Therefore, the use of detergents is virtually unlimited in chemical synthesis and processing, medicine, biological systems and agricultural biology. The article includes the studies of the feasibility of application of 1,1,1,3,5,5,5-heptamethyltrisiloxane modified by polyalkylene oxide in combination with allylox
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32

Aboubakr, Esam M., Hamdoon A. Mohammed, Abeer S. Hassan, Hebatallah B. Mohamed, Mahmoud I. El Dosoky, and Adel M. Ahmad. "Glutathione-loaded non-ionic surfactant niosomes: A new approach to improve oral bioavailability and hepatoprotective efficacy of glutathione." Nanotechnology Reviews 11, no. 1 (2021): 117–37. http://dx.doi.org/10.1515/ntrev-2022-0010.

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Abstract A new formulation (niosomes) was prepared to enhance the bioavailability, hepatic tissue uptake, and hepatoprotective activity of glutathione (GSH). The GSH-loaded niosomes (nanoform, N-GSH) were formulated by the thin-film hydration technique using cholesterol/non-ionic surfactants (Span®40, Span®60, and Tween®80) at a componential ratio of 1:1 and 2:1. The hepatoprotective activity of N-GSH, GSH, and the standard silymarin against CCl4-induced liver damage and oxidative stress were tested on the rats’ model. The hepatic morphology and histopathological characters were also investiga
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Al-Qaisi, Talal Salem, Ahmed A.J. Jabbar, Muhsin H. Ubeid, et al. "A traditional medicinal food Arum rupicola (Kardeh) ameliorates thioacetamide-induced hepatotoxicity in animal model." Italian Journal of Food Science 36, no. 4 (2024): 208–28. http://dx.doi.org/10.15586/ijfs.v36i4.2723.

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Nutraceuticals are major contributors to human health because of their modulatory effects on various physiological and pathological processes. Arum rupicola (Kardeh) is a traditional medicinal food used for many gastrointestinal and enzymatic disorders. The present study evaluates the acute toxicity and hepatoprotective effects of methanolic extracts of Arum rupicola (MEAR) in rat with thioacetamide (TAA)-induced liver injury in rat. Thirty Sprague-Dawley rats were divided into five groups: Group A, normal control, and group B, TAA control groups were treated orally with 10% tween 20; group C
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Khalil-Zada, R. S., S. A. Etibarli, and K. T. Hepgul. "SURGERY FOR OCCULT SPINAL DYSRAPHISM." National Journal of Neurology 2013, Special (2013): 132–33. http://dx.doi.org/10.61788/njn.spec.13.30.

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Failure of closure of the spine is referred to as spinal dysraphism or spina bifida. If there is no, or minimal soft tissue defect so that neural tissue is not exposed, it is referred to as occult spinal dysra-phism (OSD). Most OSD include become evident as tethered cord syndrome or in rare cases as diastematomyelia. Surgical detethering is the only treatment option, as natural history shows neurological deterioration in most cases with non-surgical treatment. In this report, we analyzed results of surgical treatment for OSD in seventeen patients operated be-tween 2008-2013 depending on their
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Amantayev, Nurbolat Gabdullaevich, Marina Mikhailovna Silantyeva, and Vadim Tagirovich Khassanov. "Selecting sterilization conditions for the explants of different potato varieties to be in vitro introduced into the culture." Bulletin of the Karaganda University. “Biology, medicine, geography Series” 111, no. 3 (2023): 16–24. http://dx.doi.org/10.31489/2023bmg3/16-24.

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An explant is a fragment of plant tissue or organ that is incubated on its own or used to produce a primary callus. The introduction of any plant’s tissues into the culture in vitro begins with the selection of an uninfected viable explant. The authors presented the results of the effect of various sterilizing agents as well as the treatment timing on the viability of plant explants during microclonal propagation of potatoes of the following varieties: Aladin, Gala, Nevsky, Udacha and Kostanay Novosti. The meristem sprouts from potato tubers were used as parent material. The following commerci
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Somtrakoon, Khanitta, Wilailuck Khompun, Chonlada Dechakiatkrai Theerakarunwong, and Waraporn Chouychai. "Gibberellic Acid and Tween 20 Increases Napier Grass Tolerance to Synthetic Pyrethroid." Pertanika Journal of Tropical Agricultural Science 46, no. 4 (2023): 1391–405. http://dx.doi.org/10.47836/pjtas.46.4.20.

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The wide use of synthetic pyrethroids has increased their contamination in agricultural soil, so removing this pollutant from agricultural sites is necessary. Phytoremediation offers promise for agricultural soil decontamination as it is an environmentally friendly and green method. In this study, Napier grass cv. Pakchong 1 (Pennisetum purpureum x Pennisetum Americanum), cuttings with or without soaking in gibberellic acid (GA3) with and without the surfactant Tween 20, were planted in synthetic pyrethroid contaminated soil for 20 days. The results showed that the synthetic pyrethroid reduced
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Zampieri, Sandra, Anna Ghirardello, Andrea Doria, et al. "The use of Tween 20 in immunoblotting assays for the detection of autoantibodies in connective tissue diseases." Journal of Immunological Methods 239, no. 1-2 (2000): 1–11. http://dx.doi.org/10.1016/s0022-1759(00)00168-x.

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Duduk, Natasa, Mirko Ivanovic, and Bojan Duduk. "Detection of Colletotrichum acutatum latent infections in strawberry petioles and leaves." Pesticidi i fitomedicina 23, no. 4 (2008): 235–41. http://dx.doi.org/10.2298/pif0804235d.

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Colletotrichum acutatum is the most significant agent of anthracnose strawberry fruit rot. Besides being a necrotrophic pest, it can spend a part of its life cycle as an epiphyte, in a form of latent infection. The presence of the fungi on symptomless plant tissue is considered one of the main ways of distribution of this economically harmful pathogen in the world. Investigation of latent C. acutatum infection was carried out on artificially inoculated strawberries. The initiation of fungi sporulation on symptomless petioles and leaves was carried out by exposing them to the herbicide paraquat
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Hardarani, Nofia, and Chatimatun Nisa. "Efektivitas Formulasi Sterilan terhadap Jenis Eksplan pada Kultur Durian Lahung (Durio dulcis)." Daun: Jurnal Ilmiah Pertanian dan Kehutanan 9, no. 2 (2022): 161–76. http://dx.doi.org/10.33084/daun.v9i2.4264.

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Durian lahung is a type of endemic durian to Kalimantan which has a unique characteristic, namely its red skin. The existence of this plant is almost extinct so that conservation needs to be done and one way is to use tissue culture techniques. As first step, it is necessary to conduct experiments to obtain the right sterilization method in order to obtain aseptic planting material. This study aims to determine the effectiveness of sterilant formulation on type of explant for lahung durian culture. This study was designed using two factors factorial Completely Randomized Design (CRD) and was r
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Ghassami, Erfaneh, Jaleh Varshosaz, Mohsen Minaiyan, Mehrab Nasirikenari, and Seyed M. Hoseini. "Biodistribution, Safety and Organ Toxicity of Docetaxel-Loaded in HER-2 Aptamer Conjugated Ecoflex® Nanoparticles in a Mouse Xenograft Model of Ovarian Cancer." Recent Patents on Nanotechnology 13, no. 1 (2019): 49–58. http://dx.doi.org/10.2174/1872210513666181128162403.

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Background:Docetaxel is a notably efficient anticancer drug administered for several types of malignancies including ovarian cancer. However, various side effects caused either by the nonspecific distribution of the active ingredient or by high contents of Tween 80 and ethanol in the currently marketed formulations, could even deprive the patients of the treatment.Objectives:In the current study, a novel targeted delivery system composed of Ecoflex® polymeric nanoparticles loaded with docetaxel and equipped with HER-2 specific aptamer molecules was evaluated regarding blood and tissue toxicity
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Rubio-Osornio, Moisés, Carmen T. Goméz-De León, Sergio Montes, et al. "Repurposing Simvastatin in Parkinson’s Disease Model: Protection Is throughout Modulation of the Neuro-Inflammatory Response in the Substantia nigra." International Journal of Molecular Sciences 24, no. 13 (2023): 10414. http://dx.doi.org/10.3390/ijms241310414.

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Parkinson’s disease is a neurodegenerative disorder characterized by oxidative stress and immune activation in the nigro-striatal pathway. Simvastatin regulates cholesterol metabolism and protects from atherosclerosis disease. Simvastatin-tween 80 was administered 7 days before sterotaxic intrastriatal administration of MPP+ (1-methyl-4-phenylpyridine) in rats. Fluorescent lipidic product formation, dopamine levels, and circling behavior were considered damage markers. Twenty-four hours and six days after, the animal group lesioned with MPP+ showed significant damage in relation to the control
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Molinaro, Roberto, Agnese Gagliardi, Antonia Mancuso, et al. "Development and In Vivo Evaluation of Multidrug Ultradeformable Vesicles for the Treatment of Skin Inflammation." Pharmaceutics 11, no. 12 (2019): 644. http://dx.doi.org/10.3390/pharmaceutics11120644.

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The aim of this work was to evaluate the effect of two chemically different edge activators, i.e., Tween® 80 and sodium deoxycholate, on (i) the physical, mechanical, and biological properties of ultradeformable vesicles, and (ii) the administration of naproxen sodium-loaded multidrug ultradeformable vesicles for the transdermal route in order to obtain therapeutically meaningful drug concentrations in the target tissues and to potentiate its anti-inflammatory effect by association with the antioxidant drug idebenone. The results obtained in this investigation highlighted a synergistic action
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Baral, Kshitis Chandra, Sang Hoon Lee, Jae Geun Song, Seong Hoon Jeong, and Hyo-Kyung Han. "Improved Therapeutic Efficacy of MT102, a New Anti-Inflammatory Agent, via a Self-Microemulsifying Drug Delivery System, in Ulcerative Colitis Mice." Pharmaceutics 15, no. 12 (2023): 2720. http://dx.doi.org/10.3390/pharmaceutics15122720.

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MT-102 is a new anti-inflammatory agent derived from Juglans mandshurica and Isatis indigotica. Its therapeutic potential is hindered by low aqueous solubility, impacting its in vivo efficacy. Therefore, this study aimed to develop a self-microemulsifying drug delivery system (SMEDDS) for MT-102 to enhance its oral efficacy in treating ulcerative colitis. Solubility assessment in different oils, surfactants, and cosurfactants led to a SMEDDS formulation of MT-102 using Capmul MCM, Tween 80, and propylene glycol. Based on a pseudoternary phase diagram, the optimal SMEDDS composition was selecte
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44

Anderson, Jeffrey A. "Heat Stability of Pepper Leaf Extracts." Journal of the American Society for Horticultural Science 131, no. 1 (2006): 17–23. http://dx.doi.org/10.21273/jashs.131.1.17.

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Acute high-temperature stress affects plant protein structure, leading to denaturation and aggregation. Although folding states of individual proteins have been extensively studied, little information is available on protein thermostability in complex mixtures. The objective of this study was to systematically examine protein stabilizing and destabilizing factors in pepper (Capsicum annuum L.) leaf extracts using light transmission measurements. Increasing turbidity and subsequent precipitation were monitored in heated extracts as changes in light scattering at 540 nm. Factors evaluated includ
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Archbold, Douglas D., and F. G. Dennis. "Strawberry Receptacle Growth and Endogenous IAA Content as Affected by Growth Regulator Application and Achene Removal." Journal of the American Society for Horticultural Science 110, no. 6 (1985): 816–20. http://dx.doi.org/10.21273/jashs.110.6.816.

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Abstract Parthenocarpy was induced in emasculated strawberry (Fragaria × ananassa Duch.) flowers with aqueous solutions of 10−3M NAA, GA3 or GA4+7 in 2% DMSO plus 0.1% Tween 80. All fruit except those treated with NAA stopped growing within 12 days of treatment. Repeat application with NAA or GA4+7 20 days after initial treatment stimulated continued growth of NAA-induced fruit, but had little or no effect on growth of GA4+7-induced fruit. The diameters of mature parthenocarpic fruit ranged from 70% to 90% of that of pollinated fruit. Achene removal 12 days after pollination greatly reduced su
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Widawati, Tuti, Sri Agus Sudjarwo, and Herry Agoes Hermadi. "Protective Effect of Propolis Extract Against Lead Acetate Toxicity in Mice (Mus Musculus) Testes." KnE Life Sciences 3, no. 6 (2017): 557. http://dx.doi.org/10.18502/kls.v3i6.1183.

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This study was aimed to investigate the protective effect of propolis extract from Apis mellifera that was obtained from Agro Tawon Rimba Raya Malang against the exposure of lead acetate 20 mg/kgBW orally. Twenty-five BALB/C mice were randomly devided into five groups. Negative control received only CMC-Na 1.5 % and Tween 80 0.5% also aquadest an hour after the first administration; Positive control group that administered CMC-Na 1.5 % and Tween 80 0.5% then continued received 20 mg/kgBW of lead acetate, Treatment group was received 200; 400 and 800 mg/kgBW of propolis first and then an hour a
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El-Zaafarany, Ghada, Mahmoud Soliman, Samar Mansour, et al. "A Tailored Thermosensitive PLGA-PEG-PLGA/Emulsomes Composite for Enhanced Oxcarbazepine Brain Delivery via the Nasal Route." Pharmaceutics 10, no. 4 (2018): 217. http://dx.doi.org/10.3390/pharmaceutics10040217.

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The use of nanocarrier delivery systems for direct nose to brain drug delivery shows promise for achieving increased brain drug levels as compared to simple solution systems. An example of such nanocarriers is emulsomes formed from lipid cores surrounded and stabilised by a corona of phospholipids (PC) and a coating of Tween 80, which combines the properties of both liposomes and emulsions. Oxcarbazepine (OX), an antiepileptic drug, was entrapped in emulsomes and then localized in a poly(lactic acid-co-glycolic acid)-poly(ethylene glycol)-poly(lactic acid-co-glycolic acid) (PLGA-PEG-PLGA) trib
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48

Mosaad, Rehab M., Marwan A. Ibrahim, and Hend A. Sabry. "The Prophylactic Role of Chrysin against Clonazepam Induced Brain Toxicity in Male Albino Rats." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 12 (2024): 261–68. http://dx.doi.org/10.56557/upjoz/2024/v45i124125.

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Background: The bioactive substance chyrus is found in bee propolis and all plants of the species Passiflora. It is well recognized to have neuroprotective properties and a wide range of pharmacological activity. Purpose: In this work, the consequence of Chrysin administration (50 mg/kg b.wt/day) on brain toxicity caused by Clonazepam (CZP, 2mg/kg b.wt/day) were investigated by measuring NaK ATPase, neuronal oxidative stress, neuro- inflammation, and DNA fragmentation. Study Design: In our investigation, we used male albino rats 4 weeks old and weighing 60 ± 5 g. There were four groups of ten
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Al-Qurtas, Mohammed A. M. "Immunohistochemical Targeting of p110β Isoform of phosphatidylinositol 3-kinase co-associated with Cyclin-Dependent Kinase 1 in a Group of Tissues from Iraqi Patients with Breast Cancer". AL-Kindy College Medical Journal 13, № 2 (2019): 127–36. http://dx.doi.org/10.47723/kcmj.v13i2.109.

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Background: While two-thirds of breast cancers express hormone receptors for either estrogen (ER) and/or progesterone (PR) , genetically altered PI3K pathway was found in more than 70% of ER-positive breast cancers.An aberrant activity of cyclin-dependent kinase 1 (CDK1) in a wide variety of human cancers has selectively constituted an attractive pharmacological targets in MYC-dependent human breast cancer cells.
 Aim of the study: Role of p110-beta as well as and CDK 1 in the pathogenesis of subset of breast cancers and contribution in their carcinogenesis.
 Type of the study: is a
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Surya, Muhammad Imam, and Lily Ismaini. "Perbandingan Metode Sterilisasi Untuk Perbanyakan Rubus rosifolius Secara in Vitro." Al-Kauniyah: Jurnal Biologi 14, no. 1 (2021): 127–37. http://dx.doi.org/10.15408/kauniyah.v14i1.16325.

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AbstrakRubus rosifolius adalah salah satu jenis rasberi liar yang memiliki potensi cukup tinggi untuk dikembangkan sebagai tanaman buah. Selain itu, metode perbanyakan tanaman merupakan salah satu faktor yang berpengaruh dalam pembudidayaan. Lebih lanjut, informasi terkait upaya perbanyakan R. rosifolius secara in vitro masih sangat terbatas. Percobaan ini ditujukan untuk mengetahui metode sterilisasi yang tepat pada eksplan R. rosifolius. Sebanyak 17 metode sterilisasi telah diujicobakan di Laboratorium Kultur Jaringan BKT Kebun Raya Cibodas-LIPI. Bahan sterilisasi yang digunakan, yaitu deter
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