Relevant bibliographies by topics / Two-Hybrid screening

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Two-Hybrid screening'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Two-Hybrid screening"

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Koegl, M., and P. Uetz. "Improving yeast two-hybrid screening systems." Briefings in Functional Genomics and Proteomics 6, no. 4 (2008): 302–12. http://dx.doi.org/10.1093/bfgp/elm035.

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Parchaliuk, Debra L., Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, and R. Daniel Gietz. "Yeast two-hybrid system: part B—screening procedure." Technical Tips Online 4, no. 1 (1999): 30–34. http://dx.doi.org/10.1016/s1366-2120(08)70133-8.

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Lecrenier, Nicolas, Françoise Foury, and Andre Goffeau. "Two-hybrid systematic screening of the yeast proteome." BioEssays 20, no. 1 (1998): 1–5. http://dx.doi.org/10.1002/(sici)1521-1878(199801)20:1<1::aid-bies2>3.0.co;2-y.

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Helmuth, Martin, Wilko Altrock, Tobias M. Böckers, Eckart D. Gundelfinger, and Michael R. Kreutz. "An Electrotransfection Protocol for Yeast Two-Hybrid Library Screening." Analytical Biochemistry 293, no. 1 (2001): 149–52. http://dx.doi.org/10.1006/abio.2001.5107.

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Bhartur, Sheela G., and James R. Goldenring. "Mapping of Ezrin Dimerization Using Yeast Two-Hybrid Screening." Biochemical and Biophysical Research Communications 243, no. 3 (1998): 874–77. http://dx.doi.org/10.1006/bbrc.1998.8196.

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Vinayagam, Arunachalam, Ulrich Stelzl, and Erich E. Wanker. "Repeated two-hybrid screening detects transient protein–protein interactions." Theoretical Chemistry Accounts 125, no. 3-6 (2009): 613–19. http://dx.doi.org/10.1007/s00214-009-0651-8.

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Vidal, Marc, and Hideki Endoh. "Prospects for drug screening using the reverse two-hybrid system." Trends in Biotechnology 17, no. 9 (1999): 374–81. http://dx.doi.org/10.1016/s0167-7799(99)01338-4.

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Yu, Z., N. Liu, Y. Wang, X. Li, and X. Wang. "Identification of neuroglobin-interacting proteins using yeast two-hybrid screening." Neuroscience 200 (January 2012): 99–105. http://dx.doi.org/10.1016/j.neuroscience.2011.10.046.

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Lina, Zhou, Wu Jun, Luo Gaoxing, et al. "Screening of FOXP3-interacted proteins by yeast two-hybrid technique." Journal of Medical Colleges of PLA 23, no. 2 (2008): 81–87. http://dx.doi.org/10.1016/s1000-1948(08)60027-1.

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YU, Ying. "Screening of BRD7 interacting proteins by yeast two-hybrid system." Science in China Series C 45, no. 5 (2002): 546. http://dx.doi.org/10.1360/02yc9060.

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Dissertations / Theses on the topic "Two-Hybrid screening"

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Patrício, Daniela Marques. "Implementation of mammalian two-hybrid system for highthroughput drugs screening." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22327.

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Mestrado em Biologia Molecular e Celular<br>The base of cell communication is signalling pathways which, when deregulated can lead to several diseases, from cancer to autoimmune diseases. Protein-protein interactions (PPIs) are the backbone of signalling pathways. Consequently, identifying and modulating PPIs can unravel the pathophysiological mechanisms of a disease. When considering the male reproductive system, the same is observed, hence PPIs are responsible and regulate most of the cellular functions. PPP1CC2 is a testis enriched protein, key to sperm motility acquisition. Its interaction with AKAP4, also a testis-specific protein, appears to be a potential new drug target. Therefore, the modulation of PPP1CC2/AKAP4 interaction under mammalian two-hybrid system is crucial. Two-hybrid system, particularly the mammalian two-hybrid (MTH), is an efficient technique to validate PPIs ex vivo. Thus, we will explore this tool for highthroughput drug screening through the modulation of PPIs. Consequently, it will be used to identify either a target to a specific drug or drugs. This methodology relies on the fact that in eukaryotes, gene transcription only occurs when the DNA-binding domain (BD) and DNA activation domain (AD) of a transcription factor come into close proximity. When fusing the cDNA of two interacting proteins with the BD and AD, a reporter gene is expressed and the interaction can be detected. The main goal of this thesis is to implement the MTH into the Signal Transduction laboratory and to be able to identify and modulate PPIs in a system that can mimic the natural way PPI’s occur – mammalian cells.<br>A comunicação celular baseia-se em vias de sinalização, que quando desreguladas podem levar ao desenvolvimento de doenças, desde cancro a doenças autoimunes. As interações proteína-proteína (PPIs) são fundamentais para a regulação de vias de sinalização. Deste modo, a identificação e a modulação de PPIs é crucial para entender mecanismos pato-fisiológicos de diferentes doenças. O sistema reprodutor masculino não é exceção, estando as PPIs no centro da manutenção de funções celulares na produção e funcionamento dos gâmetas. A PPP1CC2 é uma proteína com expressão enriquecida nos testículos e a sua interação com a AKAP4, proteína específica de testículo, aparenta ser um bom alvo para novos fármacos. A monitorização da modulação da interação PPP1CC2/AKAP4 através do sistema de dois-híbrido em mamífero (MTH) é crucial. Os sistemas dois-híbrido são técnicas específicas para validar PPIs ex vivo. Desta forma, neste trabalho explorou-se o sistema MTH para uma análise de potenciais drogas para a modulação de PPIs. Isto permitirá identificar um novo alvo terapêutico e/ou desenvolvimento de drogas que afetem especificamente uma PPI. Esta metodologia baseia-se no facto de em eucariotas, a transcrição de genes ocorrer apenas quando o domínio de ligação (BD) e o domínio de ativação (AD) do DNA estão próximos. Ao fundir o DNA complementar de duas proteínas que interagem com o BD e AD, a expressão de um gene repórter é ativada e a interação detetada. O objetivo principal desta tese é implementar o MTH no laboratório de transdução de sinal, para identificar e modular PPIs num sistema que mimetiza o ambiente onde ocorrem PPIs – células de mamíferos.
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Hung, Kwok Wang. "Identification of the EphA4-interacting proteins by yeast two-hybrid screening /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20HUNG.

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Zhang, Linhua 1968. "Identification of Fyb as an interacting protein with CD45 in yeast two-hybrid screening." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98530.

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CD45, also known as Leukocyte-common antigen (LCA), is a type-1 receptor-like protein tyrosine phosphatase (PTPase), which contains two PTPase domains, the membrane proximal domain (DI) and the membrane distal domain (D2). It is uniquely expressed on the surface of all leukocytes and their hematopoietic precursors and plays an important role in immunoreceptor signaling. In this study, I identified a novel CD45-interacting protein, Fyb, through screening human leukocyte cDNA library with a modified yeast two-hybrid system. Fyb is an adaptor protein mainly involved in the regulation of T cell receptor-induced integrin clustering and adhesion. It contains several regions with potential to mediate protein-protein interactions and has been shown to bind to the Src family kinase Fyn, the adapter protein SLP-76, Ena/VASP proteins, the adapter protein SKAP55, and SKAP55-HOM in T cell receptor signaling. I also demonstrated that the interaction of Fyb with CD45 was through involves the C-terminal region of Fyb, where several tyrosine residues, such as Tyr 594 and Tyr624, are critical for its interaction with other proteins in signaling. The interaction of Fyb with CD45 is phosphotyrosine-dependent since Src kinase is required for the interaction. Mutation analysis revealed that the tyrosine residue 624 of Fyb is the pivotal tyrosine residue involved in its association with CD45, whereas tyrosin 594 was not found to be required for binding. By mutation of CD45, it was demonstrated that the phosphatase catalytic D1 domain of CD45 was involved in the interaction with Fyb. More significantly, I found that only the substrate-trapping mutant (Asp to Val), but not the wild type CD45 interacted with Fyb, suggesting that Fyb is a potential CD45 substrate. Furthermore, I demonstrated the association of the C-terminal fragment of Fyb with CD45 in mammalian cells. However, I was not able to determine the interaction of the wild type Fyb with CD45 in 293 cells.
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Farzadfar, Rahin. "Identification of Artemis and PARP-1 interacting factors: A yeast two hybrid screening approach." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28481.

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The genomic integrity of the cell is under the constant threat of DNA damage from endogenous and exogenous sources. In response to DNA damage mammalian cells elicit a highly complex, yet coordinated series of cellular responses, which are triggered by the detection of DNA lesions and ultimately lead to cell survival (DNA repair) or apoptosis. Artemis and PARP-1 are two proteins with significant implications in the various branches within the DNA damage response network. A series of yeast two hybrid screening (Y2H) experiments were conducted using both classical and interacting mating Y2H systems to find Artemis and PARP-1 interacting factors. The ATRX protein was identified as a putative interactor of Artemis, Caspase-7 and an "unkown" protein were identified as putative interactors of PARP-1 binding factors. In addition a rare case of false positives were identified and characterized. Furthermore, the strengths and limits of each Y2H system, as well as troubleshooting techniques developed to increase the efficiency of each system were thoroughly examined.
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Friese, Anke. "Etablierung eines Zwei-Hybrid-Screening-Systems zur Suche und Charakterisierung von Ras-Raf-Effektoren." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964787466.

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Scott, Adam. "Modified yeast two-hybrid screening identifies SKAP-HOM as a novel substrate of PTP-PEST." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21986.

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PTP-PEST is a soluble intracellular phosphatase that is ubiquitously expressed in mammalian tissues. The biological importance of this enzyme is highlighted by the fact that it is essential for murine embryogenesis and that it has been implicated in actin dynamics as well as other processes such as immunoreceptor signalling and cell death. Having five proline-rich regions in its C-terminal tail, PTP-PEST has a large potential for protein:protein interactions, several of which are known. To better understand the physiological importance of PTP-PEST, we explored the possibility that other substrates or binding partners existed. To this end, we carried out a modified yeast two-hybrid assay using full-length PTP-PEST with the D199A substrate trapping mutation as bait and screened a library of cDNA clones from 17-day-old mouse embryos. Screening identified the cytosolic adaptor protein, SKAP-HOM, as a novel substrate of PTP-PEST and GST pull-down assays confirmed this interaction in HeLa299 cells. We showed that PTP-PEST's PTP-domain and proline-1 region are essential for this interaction, as are SKAP-HOM's Y260 residue and SH3 domain. Further mutagenesis studies revealed that PTP-PEST residues S39 and S434 play a role in inhibiting and promoting the interaction with SKAP-HOM, respectively. Preliminary evidence was also obtained suggesting that p59fyn kinase plays a role in promoting the PTP-PEST D199A:SKAP-HOM interaction through a mechanism other than SKAP-HOM phosphorylation. Understanding the biological significance of this interaction could lead to novel discoveries in the fields of cell metastasis and inflammation.<br>PTP-PEST est une phosphatase soluble intracellulaire qui est exprimée de façon ubiquitaire dans les tissus de mammifères. L'importance biologique de cette enzyme relève du fait qu'elle est essentielle durant l'embryogénèse chez la souris, et qu'elle est de plus impliquée dans l'organisation dynamique du cytosquelette d'actine, ainsi que dans d'autres processus tels la signalisation par les immunorécepteurs et la mort cellulaire. Avec ses cinq régions riches en motifs proline dans la région C-terminale, PTP-PEST possède un large potentiel d'interactions protéine : protéine, parmi lesquelles plusieurs sont déjà identifiées. Afin de mieux comprendre l'importance physiologique de PTP-PEST, nous avons exploré la possibilité de l'existence d'autres substrats ou ligands. À cette fin, nous avons développé un test modifié de levures à deux-hybrides, utilisant une construction pleine longueur de PTP-PEST insérée d'une mutation de piégeage de ligands D199A comme appât. Suite à l'analyse d'une banque d'ADNc provenant d'embryons de souris de 17 jours, nous avons identifié une protéine adaptatrice cytosolique, SKAP-HOM, en tant que nouveau substrat de PTP-PEST. L'essai de capture de la protéine GST a de plus confirmé cette interaction dans les cellules HeLa299. Nos résultats démontrent que le domaine PTP de PTP-PEST, ainsi que la région proline-1, sont essentiels à cette interaction, tout comme le résidu Y260 et le domaine SH3 de SKAP-HOM. Une analyse de mutagénèse plus approfondie a identifié les résidus S39 et S434 de PTP-PEST comme jouant respectivement un rôle dans l'inhibition et dans la promotion de l'interaction avec SKAP-HOM. Nous avons aussi obtenus des résultats préliminaires suggérant que la protéine kinase p59fyn joue aussi un rôle dans la promotion de l'interaction PTP-PEST D199A:SKAP-HOM, par un mécanisme autre que la phosphorylation de SKAP-HOM. La compréhension de l'importance biologique de cette interaction pour
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Davidora, Albena. "Validation and characterization of putative NHE6-interacting proteins identified by yeast two-hybrid screening and tandem affinity purification :." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100793.

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Na+/H+ exchangers (NHE) are integral membrane proteins that catalyze the electroneutral exchange of Na+ (or K+) for H+. The nine human isoforms identified to date share the same topology of twelve membrane-spanning domains and a cytoplasmic regulatory tail, and diverge in their tissue expressions and subcellular localizations. This thesis focused on the identification and characterization of proteins interacting with the ubiquitous NHE6 isoform, which resides predominantly in recycling endosomes. Recent yeast two-hybrid (Y2H) screening of a human brain cDNA library using the regulatory tail of NHE6 as a probe resulted in the tentative identification of about 250 partial or full-length NHE6-interacting proteins. Thirty other potential NHE6-interacting partners were identified by isolating NHE6 complexes from embryonic kidney 293 cells by tandem affinity purification (TAP) and subjecting them to mass spectrometry analysis. The interaction between NHE6 and eight of these proteins (four from each Y2H and TAP analyses) was tested by co-immunoprecipitation, co-localization by immunofluorescence microscopy and in vitro GST-fusion protein pull-down assays. We show that apolipoprotein D interacts weakly with NHE6, while myosin light chain kinase and the mitochondrial ATP synthase subunit-alpha are true NHE6-binding partners. The remaining five proteins exhibited poor and/or non-reproducible association with NHE6.
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Alzahrani, Ashwag. "Identification of Human Proteins Interacting with the Protein IcsB of Shigella flexneri." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38333.

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Elfaki, Imadeldin [Verfasser], Peter [Akademischer Betreuer] Bayer, and Michael [Akademischer Betreuer] Ehrmann. "Binding Partners of Parvulin Proteins using the High-Throughput Screening Methods such as Yeast two-hybrid and Phage Display / Imadeldin Elfaki. Gutachter: Michael Ehrmann. Betreuer: Peter Bayer." Duisburg, 2011. http://d-nb.info/1016615027/34.

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Pow, Andrew James. "Protein complementation assay as a display system for screening protein libraries in the intracellular environment." Queensland University of Technology, 2008. http://eprints.qut.edu.au/30392/.

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A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precursor protein), and various proteins crucial to viral replication (e.g. HIV-1 proteins such as Tat, Rev and Vif-1), which are difficult to screen by phage, ribosome or cell-surface display. This study used the â-lactamase protein complementation assay (PCA) as the display and selection component of a system for screening a protein library in the cytoplasm of HEK 293T cells. The colicin E7 (ColE7) and Immunity protein 7 (Imm7) Escherichia coli proteins were used as model interaction partners for developing the system. These proteins drove effective â-lactamase complementation, resulting in a signal-to-noise ratio (9:1 – 13:1) comparable to that of other â-lactamase PCAs described in the literature. The model Imm7-ColE7 interaction was then used to validate protocols for library screening. Single positive cells that harboured the Imm7 and ColE7 binding partners were identified and isolated using flow cytometric cell sorting in combination with the fluorescent â-lactamase substrate, CCF2/AM. A single-cell PCR was then used to amplify the Imm7 coding sequence directly from each sorted cell. With the screening system validated, it was then used to screen a protein library based the Imm7 scaffold against a proof-of-principle target. The wildtype Imm7 sequence, as well as mutants with wild-type residues in the ColE7- binding loop were enriched from the library after a single round of selection, which is consistent with other eukaryotic screening systems such as yeast and mammalian cell-surface display. In summary, this thesis describes a new technology for screening protein libraries in a mammalian, intracellular environment. This system has the potential to complement existing screening technologies by allowing access to intracellular proteins and expanding the range of targets available to the pharmaceutical industry.
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Book chapters on the topic "Two-Hybrid screening"

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Maple, Jodi, and Simon G. Møller. "Yeast Two-Hybrid Screening." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-257-1_15.

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Schenk, Jörg A. "Two Hybrid cDNA Cloning." In Genetic Library Construction and Screening. Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_8.

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Donnard, Elisa, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz. "Yeast Two-Hybrid Liquid Screening." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0799-1_7.

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Roberts, George G., Jodi R. Parrish, Bernardo A. Mangiola, and Russell L. Finley. "High-Throughput Yeast Two-Hybrid Screening." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-455-1_3.

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Huang, Betty C. B., and Ying Luo. "Yeast One and Two Hybrid cDNA Cloning." In Genetic Library Construction and Screening. Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_9.

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Mohr, Kerstin, and Manfred Koegl. "High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-455-1_5.

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Dudha, Namrata, and Sanjay Gupta. "Viral–Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3618-2_15.

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Grein, Swen, Karine Raymond, Claude Cochet, Walter Pyerin, Edmond M. Chambaz, and Odile Filhol. "Searching interaction partners of protein kinase CK2β subunit by two-hybrid screening." In A Molecular and Cellular View of Protein Kinase CK2. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4419-8624-5_13.

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Barreto, Kris, and C. Ronald Geyer. "Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0799-1_21.

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Letteboer, Stef J. F., and Ronald Roepman. "Versatile Screening for Binary Protein-Protein Interactions by Yeast Two-Hybrid Mating." In Functional Proteomics. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-398-1_10.

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Conference papers on the topic "Two-Hybrid screening"

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Polterauer, Stephan, Dharmarao Thapi, Nikolaus Schultz, et al. "Abstract 3033: Identification of the MUC16/CA125 interaction network: Results of a high-throughput yeast two-hybrid screening." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3033.

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Polterauer, Stephan, Ryan Parlett, Dharmarao Thapi, Nestor Rosales, David R. Spriggs та Xiu J. Yan. "Abstract 1946: Detecting protein-protein interactions between MUC16 CDΔ102 (80AA) and a SKOV-3 cDNA library using yeast two-hybrid screening". У Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-1946.

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Liu, Fei, Fang Li, David C. Spray, Anis Nurashikin Nordin, and Ioana Voiculescu. "High Sensitivity MEMS Biosensor for Monitoring Cell Attachment." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-85613.

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This paper presents the fabrication and testing of a novel microelectromechanical (MEMS) biosensor based on live cells. The biosensor combines two biosensing techniques; resonant frequency measurements and electric cell-substrate impedance sensing (ECIS) on a single device. The sensor is based on the innovative placement of the working microelectrode for ECIS technique as the upper electrode of a quartz crystal microbalance (QCM) resonator. This hybrid biosensor was tested with bovine aortic endothelial cells with different seeding densities. The cell attachment and spreading was monitored with both sensors; the QCM and the ECIS technique. After the cells form a monolayer the values of the impedance and resonant frequency measurements are constant. The optimal cell seeding density with minimal time required to attach and form a monolayer was observed to be 1.5×104 cells/cm2. This biosensor monitors the cells attachment and viability and could be used for screening toxicants in drinking water.
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Hassan, Anas M., Mohammed Ayoub, Mysara Eissa, Hans Bruining, Abdullah Al-Mansour, and Abdulrahman Al-Quraishi. "A New Hybrid Improved and Enhanced Oil Recovery IOR/EOR Process Using Smart Water Assisted Foam SWAF Flooding in Carbonate Rocks; A Laboratory Study Approach." In International Petroleum Technology Conference. IPTC, 2021. http://dx.doi.org/10.2523/iptc-21381-ms.

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Abstract Given the increasing demand for energy globally and depleting oil and gas resources, it is crucial to increase the production from existing reservoirs by introducing new technologies for Improved/Enhanced Oil Recovery (IOR/EOR). This contribution presents a novel hybrid IOR/EOR method, which combines smart water (SW) and foam flooding, known as Smart Water Assisted Foam (SWAF) flooding. The optimal conditions of the SWAF technology will be interpreted using experimental laboratory design (i.e., experimental data). The experimental design was divided into three main steps. The first step is obtaining rock wettability measurements using contact angle measurements. This step aims to select the optimum SW composition that changes the carbonate rock's wettability from oil-wet towards more water-wet and faster oil recoveries. The water-wet condition leads to high residual oil saturations and low end-point permeabilities. This is conductive to favourable mobility ratios and efficient water-oil displacement. However, high residual oil saturations are unfavourable to the high ultimate oil recovery as much oil stays behind. Secondly, the chemical screening follows, where two tests were performed, viz., (i) an Aqueous Stability Test (AST), (ii) and a Foamability and Foam Stability Tests (FT/FST). This step aims to generate a stable foam (i.e., surfactant aqueous solution + gas) in the absence and presence of crude oil with different TAN (Total Acid Number) and TBN (Total Base Number), viz., crude oils Type-A and Type-B. Favourable mobility ratio is achieved by the presence of foam, which leads to excellent displacement efficiency. Thirdly, core flooding tests are performed. This step aims to select the best formulations through SWAF core flooding tests to obtain the ultimate recovery factor under different injection scenarios. The optimal SWAF condition combines high ultimate recovery with the best displacement efficiency. It is shown that the enormous changes in wettability were seen for SW (MgCl2) solution at 3500 (ppm) for both crude oils Type-A and Type-B. It has been shown that the use of a cationic surfactant CTAB (i.e., cetyltrimethylammonium-bromide) in the positively charged carbonates (with an isoelectric point of pH = 9) is more effective than the use of anionic surfactant, e.g., Alpha Olefin Sulfonate (AOS). The aim is to create an optimum surfactant aqueous solution (SAS). The SAS stability is considerably affected by the concentration of both the SW (MgCl2) and surfactant (CTAB). In the absence of oil, the strength of foam (SAS and Gas) is highly dependent on the concentration and composition of the SW in the SAS. In the presence of oil, foam generation and stability are better when the crude oil has a low TAN and high TBN. From the core flooding tests for crude oils Type-A and Type-B, the ultimate residual oil recovery was achieved by the MgCl2 - foam injection combination (i.e., incremental oil recovery of 42%, which is equivalent to a cumulative oil recovery of 92%). In summary, SWAF under the optimum conditions is a promising method to increase the oil recovery from carbonate reservoirs.
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Briones, Alejandro M., Robert Olding, Joshua P. Sykes, Brent A. Rankin, Kyle McDevitt, and Joshua S. Heyne. "Combustion Modeling Software Development, Verification and Validation." In ASME 2018 Power Conference collocated with the ASME 2018 12th International Conference on Energy Sustainability and the ASME 2018 Nuclear Forum. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/power2018-7433.

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Historically, combustion modeling is important for many transportation- and ground-based applications. More recently, modeling has been offered as an early screening tool in the evaluation of a potential alternative aviation jet fuel. This combustion evaluation path would in theory be conducted by gas turbine Original Equipment Manufacturers (OEMs) on proprietary geometries and conditions. Ideally, OEMs would have access to the latest combustion theory models and would thus have the highest predictive confidence in their model predictions. Unfortunately, the latest combustion theory codes are not written for commercial purposes. This work identifies and develops a conduit for OEM usage of latest flamelet theory for use in the evaluation of alternative jet fuel combustion properties. A so-called “common format routine” (CFR) software with two low-dimensional manifold combustion models that can be used for laminar and turbulent applications is developed, which can be implemented by OEMs on proprietary hardware. The two models are the flamelet prolongation of the intrinsic low-dimensional manifold (FPI), used for premixed combustion, and the flamelet progress variable (FPV), utilized for nonpremixed combustion. The three branches of combustion are computed using a hybrid tool that combines homotopic flamelet calculations with scaling laws and the two- and one-point flamelet continuation methods in order to resolve bifurcations. The mixture fraction and progress variable definitions can be chosen to be any summation of atomic and species composition, respectively. Diffusivity coefficients can be computed using unity Lewis number, mixture-averaged and multicomponent species composition. The turbulence-chemistry interaction is tabulated a priori using Beta probability density function (PDF) for the mixture fraction and Beta or Dirac-delta PDF for the progress variable. Parallel computing is necessary for industrial quality tabulation. The tabulated table can be used for k-ε and k-ω RANS, SAS, DES, and LES simulations. The software can also interact with liquid spray and exchange mass between the liquid and gaseous phase. The software is verified against previous numerical simulations of canonical triple flames, piloted flames and single-cup combustor. The numerical results are validated against experimental measurements of temperature and species mass fractions. The CFR software advances Cantera 2.3. Hence, the software contains an inner layer of C++ code, an intermediate layer of Python wrappers, and an upper layer (GUI) of C# code. The pre-tabulated chemistry is used for CFD simulations. The tables are bi-linearly interpolated for laminar simulations and tri-linearly interpolated for turbulent simulations. The tabulated chemistry can be hooked to commercial software such as Fluent through C and Scheme codes. The simulated flames presented here were computed with this software. The developed software is reliable for modeling and simulation of complex combustion phenomena.
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