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Dissertations / Theses on the topic 'Two-Hybrid screening'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Patrício, Daniela Marques. "Implementation of mammalian two-hybrid system for highthroughput drugs screening." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22327.

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Mestrado em Biologia Molecular e Celular<br>The base of cell communication is signalling pathways which, when deregulated can lead to several diseases, from cancer to autoimmune diseases. Protein-protein interactions (PPIs) are the backbone of signalling pathways. Consequently, identifying and modulating PPIs can unravel the pathophysiological mechanisms of a disease. When considering the male reproductive system, the same is observed, hence PPIs are responsible and regulate most of the cellular functions. PPP1CC2 is a testis enriched protein, key to sperm motility acquisition. Its interaction

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2

Hung, Kwok Wang. "Identification of the EphA4-interacting proteins by yeast two-hybrid screening /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BICH%202006%20HUNG.

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3

Zhang, Linhua 1968. "Identification of Fyb as an interacting protein with CD45 in yeast two-hybrid screening." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98530.

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CD45, also known as Leukocyte-common antigen (LCA), is a type-1 receptor-like protein tyrosine phosphatase (PTPase), which contains two PTPase domains, the membrane proximal domain (DI) and the membrane distal domain (D2). It is uniquely expressed on the surface of all leukocytes and their hematopoietic precursors and plays an important role in immunoreceptor signaling. In this study, I identified a novel CD45-interacting protein, Fyb, through screening human leukocyte cDNA library with a modified yeast two-hybrid system. Fyb is an adaptor protein mainly involved in the regulation of T cell re

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Farzadfar, Rahin. "Identification of Artemis and PARP-1 interacting factors: A yeast two hybrid screening approach." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28481.

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The genomic integrity of the cell is under the constant threat of DNA damage from endogenous and exogenous sources. In response to DNA damage mammalian cells elicit a highly complex, yet coordinated series of cellular responses, which are triggered by the detection of DNA lesions and ultimately lead to cell survival (DNA repair) or apoptosis. Artemis and PARP-1 are two proteins with significant implications in the various branches within the DNA damage response network. A series of yeast two hybrid screening (Y2H) experiments were conducted using both classical and interacting mating Y2H syste

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5

Friese, Anke. "Etablierung eines Zwei-Hybrid-Screening-Systems zur Suche und Charakterisierung von Ras-Raf-Effektoren." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964787466.

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6

Scott, Adam. "Modified yeast two-hybrid screening identifies SKAP-HOM as a novel substrate of PTP-PEST." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21986.

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PTP-PEST is a soluble intracellular phosphatase that is ubiquitously expressed in mammalian tissues. The biological importance of this enzyme is highlighted by the fact that it is essential for murine embryogenesis and that it has been implicated in actin dynamics as well as other processes such as immunoreceptor signalling and cell death. Having five proline-rich regions in its C-terminal tail, PTP-PEST has a large potential for protein:protein interactions, several of which are known. To better understand the physiological importance of PTP-PEST, we explored the possibility that other sub

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Davidora, Albena. "Validation and characterization of putative NHE6-interacting proteins identified by yeast two-hybrid screening and tandem affinity purification :." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100793.

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Na+/H+ exchangers (NHE) are integral membrane proteins that catalyze the electroneutral exchange of Na+ (or K+) for H+. The nine human isoforms identified to date share the same topology of twelve membrane-spanning domains and a cytoplasmic regulatory tail, and diverge in their tissue expressions and subcellular localizations. This thesis focused on the identification and characterization of proteins interacting with the ubiquitous NHE6 isoform, which resides predominantly in recycling endosomes. Recent yeast two-hybrid (Y2H) screening of a human brain cDNA library using the regulatory tail of

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8

Alzahrani, Ashwag. "Identification of Human Proteins Interacting with the Protein IcsB of Shigella flexneri." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38333.

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9

Elfaki, Imadeldin [Verfasser], Peter [Akademischer Betreuer] Bayer, and Michael [Akademischer Betreuer] Ehrmann. "Binding Partners of Parvulin Proteins using the High-Throughput Screening Methods such as Yeast two-hybrid and Phage Display / Imadeldin Elfaki. Gutachter: Michael Ehrmann. Betreuer: Peter Bayer." Duisburg, 2011. http://d-nb.info/1016615027/34.

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10

Pow, Andrew James. "Protein complementation assay as a display system for screening protein libraries in the intracellular environment." Queensland University of Technology, 2008. http://eprints.qut.edu.au/30392/.

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A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precurso

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Malicorne, Sébastien. "Recherche d’interactants du domaine immunosuppresseur des protéines d’enveloppe rétrovirales." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS579.

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La plupart des virus ont développé des mécanismes de résistance ou de suppression du système immunitaire pour parvenir à infecter durablement leur hôte. Ces mécanismes demeurent encore imparfaitement connus. Un domaine immunosuppresseur (IS) a été identifié au niveau de la région transmembranaire des protéines d’enveloppe des rétrovirus endogènes ou infectieux. Ce domaine hautement conservé a été décrit par exemple comme inhibant l’activation lymphocytaire. Dans le laboratoire, il a été caractérisé en particulier via des expériences de rejet de cellules tumorales in vivo, ce qui a permis de dé

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12

Kahloul, Senda. "Analyse structurale et fonctionnelle de la sous-unité SKP1 du complexe SCF (Skp1-Cullin-Fbox) chez le riz (Oryza sativa)." Thesis, Clermont-Ferrand 2, 2012. http://www.theses.fr/2012CLF22326/document.

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Chez les eucaryotes, la voie de protéolyse Ub/ protéasome 26S est responsable de la dégradation sélective de la plupart des protéines intracellulaires. Cette dégradation par le protéasome 26S est initiée par une polyubiquitination de la protéine réalisée grâce à l’action d’une cascade enzymatique impliquant 3 types d'enzymes nommées « ubiquitin-activating enzyme » (E1), « ubiquitin-conjugating enzyme » (E2) et « ubiquitin-protein ligase » (E3). Il existe différentes classes d’ubiquitines ligases (E3), parmi lesquelles la plus connue est le complexe SCF (Skp1-Cullin-F-box). La protéine SKP1 fix

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13

Hao, Yanwei. "Auxin-mediated fruit development and ripening : new insight on the role of ARFs and their action mechanism in tomato (S. lycopersicum)." Thesis, Toulouse, INPT, 2014. http://www.theses.fr/2014INPT0094/document.

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L'auxine est une hormone végétale qui coordonne plusieurs processus de développement des plantes à travers la régulation d'un ensemble spécifique de gènes. Les Auxin Response Factors (ARF) sont des régulateurs transcriptionnels qui modulent l'expression de gènes de réponse à l’auxine. Des données récentes montrent que les membres de la famille des ARF sont impliqués dans la régulation du développement des fruits de la nouaison à la maturation. L'objectif principal de la thèse est d’étudier la part qui revient aux ARF dans le contrôle du développement et de la maturation des fruits et d’en comp

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14

Yang, Po-ho, and 楊博賀. "Searching for TSG101 interacting protein by yeast two-hybrid screening." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/r4xcqk.

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碩士<br>國立中山大學<br>生物科學系研究所<br>93<br>Tumor Susceptibility Gene, TSG101, has been identified as a tumor susceptibility gene with multiple functions. TSG101 encodes a 46 kDa protein composed of 390 amino acids. As previous studies reported, TSG101 participates in cell-cycle control, membrane proteins’ trafficking, and transcriptional regulation. To identify the proteins that mediated function involved TSG101, we perform yeast two-hybrid cDNA library screening to search for TSG101-interacting proteins. A construct pAS2-1-TSG101 was used as a bait to screen a human testis cDNA library. This screening

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15

Hsueh, Shih-Hsin, and 薛仕昕. "Using RING E3 as bait to perform yeast two-hybrid screening." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/74739641799560917614.

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碩士<br>臺灣大學<br>微生物與生化學研究所<br>98<br>RING proteins are defined as quite a big family in Arabidopsis, and their functions are varied. Many of RING proteins are involved in ubiquitin/26S proteasome system, some of them are ubiquitin ligases (E3s) which can interact directly with ubiquitin-conjugating enzyme (E2) by their RING domains. These RING E3s can also specifically indentify substrates that are going to be ubiquitinated by E2. In this study, we chose RING E3s related to abiotic stress in Arabidopsis based on microarray database and use them as baits to screen Arabidopsis library cDNA. Employi

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16

Tsai, Kauo-Wan, and 蔡國旺. "The Yeast Two-Hybrid Screening For The Cofactors Interacting With Androgen Receptor." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/09626490081834045816.

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碩士<br>國立陽明大學<br>醫學生物技術研究所<br>91<br>Abstract The androgen receptor(AR), a member of the nuclear receptor superfamily, play a central role in male sexual differentiation and hepatocarcinoma cell proliferation. Like other nuclear receptors, AR exerts its transcriptional function by binding to cis-elements upstream of promoters and interacting with other transcription factors. The yeast two-hybrid assay was employed to identify androgen receptor(AR) protein partners in liver library. By using three functional domain, N-terminal transactivation, DNA binding and ligand binding, as baits,

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Chien, Chih-Ta, and 錢志達. "Identification of proteins that interact with rictor by yeast two-hybrid screening." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/55757303089361288646.

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碩士<br>國立陽明大學<br>生物化學研究所<br>93<br>The Rictor gene is 5124 bp in length, encoding a protein of 1708 amino acids. In mammalian cells, rictor accompanies mTOR and G�浘 to form the mTORC2 complex. Signaling through mTORC2 is rapamycin-insensitive and regulates phosphorylation of Akt/PKB and the organization of cytoskeleton in mammalian cell lines. However, the upstream components and the mechanism of mTORC2 signaling remain unclear. Aiming at further understanding the molecular function of rictor, this thesis research employs yeast two-hybrid screening to identify the rictor-interacting proteins. We

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18

林智鴻. "Function Analysis of CD63 by Antisense Assay and Yeast Two Hybrid System Screening." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/52309167679585886778.

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碩士<br>國立清華大學<br>生命科學系<br>89<br>Abstract In this study, we first generated a panel of mouse monoclonal antibodies against extracellular domain of human CD63, a novel member of a recently identified protein family, tetraspanins. One of these monoclonal antibodies is found to specifically bind to mouse CD63, suggesting a self-responsive immune response. We also tried to investigate the function of CD63 in two ways: (1) an inhibition of CD63 expression by transfecting inverted CD63 extracellular domain expression vector into mouse macrophage cell line, Raw264.7; (2) Yeast Two Hyb

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19

Lu, You-Sheng, and 盧祐盛. "RolB-interacting protein screening by split-ubiquitin membrane based yeast two-hybrid system." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/erdpg8.

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碩士<br>國立臺灣大學<br>生化科技學系<br>107<br>Hairy root, a syndrome induced by Agrobacterium rhizogenes, can be a potential platform for phytochemical production. rolB on Agrobacterium rhizogenes TL-DNA is crucial for hairy root growth and phytochemical accumulation. However, how rolB affect hairy root physiology remains elusive. In this study, a split-ubiquitin based membrane yeast two-hybrid system (MYTH) was constructed to screen the possible interacting proteins of RolB in Arabidopsis thaliana. Yeast co-transformants were constructed, and the known interaction between Nt14-3-3 ωII and RolB is reproduc

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20

Lin, Hsin-Ying, and 林昕瑩. "High-efficient transformation of Saccharomyces cerevisiae by electroporation: application to yeast two-hybrid screening." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/31179200711061019975.

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碩士<br>淡江大學<br>生命科學研究所碩士班<br>95<br>In part I, high efficiency transformation by electroporation is developed. Factors such as medium preparation, pretreatment of plasmid, quantities of plasmid, are investigated with six different genes YJR129C, YHR209W, EBNA2, shy A, NBS1 and KPNA2. The purpose of our protocol is to achieve ideal efficiency so that the number of transformants can saturate the library used in two-hybrid screening. This protocol has reached a efficiency of more than 2.5 x 106 /μg DNA. In part II, high efficiency transformation by electroporation is applied to yeast two-hybrid scr

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21

Lin, Ke-Jin, and 林科錦. "Screening of the Interaction Proteins of RolC by Use of Yeast Two-hybrid System." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/rvjg9x.

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碩士<br>國立臺灣大學<br>生化科技學系<br>106<br>Hairy roots are root-like tissues, which are resulted from the infection of Agrobacterium rhizogenes. During infection, Agrobacterium would transfer the T-DNA from its Ri plasmid to plant cell and randomly insert into plant chromosome. Hairy roots are characterized by high growth rate, genetic stability, and high accumulation of plant secondary metabolite, and they could be maintained in hormone-free medium. Due to these features, hairy roots are widely applied in production of high-value pharmaceutical molucules and recombinant proteins. However, still many pl

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Wu, Chih-Yun, and 吳芷芸. "A Yeast Two-Hybrid System for the Screening of Inhibitors of NEDD8 Activating Enzyme." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/67u3vw.

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23

Roos, Marcel Philipp. "Suche nach Interaktionspartnern mit dem ATP-abhängigen Kaliumkanal der Niere, ROMK, durch "Yeast-Two-Hybrid-Screening"." Doctoral thesis, 2005. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-11424.

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Protein-Protein-Interaktionen haben eine wesentliche Bedeutung bei der Regulierung verschiedenster Zellfunktionen. Sie spielen u.a. bei der Funktionssteuerung von Kanälen, Transportern und Ionenpumpen eine wesentliche Rolle. Ein PDZ-Motiv am C- terminalen Ende des ATP-abhängigen Kaliumkanals ROMK ließ mögliche Inter-aktionen mit zellulären und membran-assoziierten Proteinen erhoffen. Nach Durch-führung dreier „Yeast-Two-Hybrid“-Screens zur Identifizierung möglicher Interakt-ionspartner von ROMK kamen 17, von ihrer Funktion schon bekannte, aussichtsreiche Proteine, in die enge Auswahl. Nach wei

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Lin, Meng-Ping, and 林孟平. "I.Yeast two-hybrid screening of Saccharomyces cerevisiae JHD1II. Heterologous expression and purification of nattokinase from Bacillus subtilis TKU007." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/25390585212948136148.

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碩士<br>淡江大學<br>生命科學研究所碩士班<br>96<br>In part I, high efficiency transformation by electroporation is applied to yeast two¬- hybrid screening. JHD1/YER051W is a demethylase gene from Saccharomyces cerevisiae BY4742. The JHD1/YER051W ORF is ligated to the BD vector as the “bait”. Appropriate DNA library constructed on the AD vector was screened for “fish” when the library was transformed into yeast PJ69-4A. The interacting proteins expressed from the AD vector would be selected in the specific medium. Afterwards, more functional analysis will be done to further understand the functional role of the

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25

Roos, Marcel Philipp [Verfasser]. "Suche nach Interaktionspartnern mit dem ATP-abhängigen Kaliumkanal der Niere, ROMK, durch "Yeast-two-hybrid-Screening" / vorgelegt von Marcel Philipp Roos." 2005. http://d-nb.info/973922915/34.

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26

Hsu, Chia-Ling, and 許家綾. "Screening and Characterization of proteins that interact with the N-terminal 1-252 amino acids of Cdc13p by yeast two-hybrid system." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/02834834756191434940.

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碩士<br>國立陽明大學<br>生物藥學研究所<br>89<br>Telomeres are the specialized structure at the very ends of eukaryotic chromosomes. They are essential for the maintenance of chromosome integrity and protect chromosomes from degradation by nucleases. Loss of telomeres could lead to cell death. In Saccharomyces cerevisiae, the telomere sequence is composed of a ~300 bp of C1-3A/TG1-3 repeats plus a short single-stranded TG1-3 tail. Cdc13p is a single strand TG1-3 telomeric DNA-binding protein. It protects telomeres and regulates telomere length. The relative molecular mass of Cdc13p is about 105 kDa that

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