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1

Koegl, M., and P. Uetz. "Improving yeast two-hybrid screening systems." Briefings in Functional Genomics and Proteomics 6, no. 4 (2008): 302–12. http://dx.doi.org/10.1093/bfgp/elm035.

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2

Parchaliuk, Debra L., Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, and R. Daniel Gietz. "Yeast two-hybrid system: part B—screening procedure." Technical Tips Online 4, no. 1 (1999): 30–34. http://dx.doi.org/10.1016/s1366-2120(08)70133-8.

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3

Lecrenier, Nicolas, Françoise Foury, and Andre Goffeau. "Two-hybrid systematic screening of the yeast proteome." BioEssays 20, no. 1 (1998): 1–5. http://dx.doi.org/10.1002/(sici)1521-1878(199801)20:1<1::aid-bies2>3.0.co;2-y.

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4

Helmuth, Martin, Wilko Altrock, Tobias M. Böckers, Eckart D. Gundelfinger, and Michael R. Kreutz. "An Electrotransfection Protocol for Yeast Two-Hybrid Library Screening." Analytical Biochemistry 293, no. 1 (2001): 149–52. http://dx.doi.org/10.1006/abio.2001.5107.

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5

Bhartur, Sheela G., and James R. Goldenring. "Mapping of Ezrin Dimerization Using Yeast Two-Hybrid Screening." Biochemical and Biophysical Research Communications 243, no. 3 (1998): 874–77. http://dx.doi.org/10.1006/bbrc.1998.8196.

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6

Vinayagam, Arunachalam, Ulrich Stelzl, and Erich E. Wanker. "Repeated two-hybrid screening detects transient protein–protein interactions." Theoretical Chemistry Accounts 125, no. 3-6 (2009): 613–19. http://dx.doi.org/10.1007/s00214-009-0651-8.

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7

Vidal, Marc, and Hideki Endoh. "Prospects for drug screening using the reverse two-hybrid system." Trends in Biotechnology 17, no. 9 (1999): 374–81. http://dx.doi.org/10.1016/s0167-7799(99)01338-4.

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8

Yu, Z., N. Liu, Y. Wang, X. Li, and X. Wang. "Identification of neuroglobin-interacting proteins using yeast two-hybrid screening." Neuroscience 200 (January 2012): 99–105. http://dx.doi.org/10.1016/j.neuroscience.2011.10.046.

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9

Lina, Zhou, Wu Jun, Luo Gaoxing, et al. "Screening of FOXP3-interacted proteins by yeast two-hybrid technique." Journal of Medical Colleges of PLA 23, no. 2 (2008): 81–87. http://dx.doi.org/10.1016/s1000-1948(08)60027-1.

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10

YU, Ying. "Screening of BRD7 interacting proteins by yeast two-hybrid system." Science in China Series C 45, no. 5 (2002): 546. http://dx.doi.org/10.1360/02yc9060.

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11

Tompkins, Van, Jussara Hagen, Valerie P. Zediak, and Dawn E. Quelle. "Identification of Novel ARF Binding Proteins by Two-Hybrid Screening." Cell Cycle 5, no. 6 (2006): 642–47. http://dx.doi.org/10.4161/cc.5.6.2560.

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12

Albers, Michael, Harald Kranz, Ingo Kober, et al. "Automated Yeast Two-hybrid Screening for Nuclear Receptor-interacting Proteins." Molecular & Cellular Proteomics 4, no. 2 (2004): 205–13. http://dx.doi.org/10.1074/mcp.m400169-mcp200.

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13

Feilotter, H. E., G. J. Harmon, C. J. Ruddell, and D. Beach. "Construction of an improved host strain for two hybrid screening." Nucleic Acids Research 22, no. 8 (1994): 1502–3. http://dx.doi.org/10.1093/nar/22.8.1502.

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14

Woloschuk, Ryan M., P. Maximilian M. Reed, Sherin McDonald, Maruti Uppalapati, and G. Andrew Woolley. "Yeast Two-Hybrid Screening of Photoswitchable Protein–Protein Interaction Libraries." Journal of Molecular Biology 432, no. 10 (2020): 3113–26. http://dx.doi.org/10.1016/j.jmb.2020.03.011.

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15

Durfee, Tim, Olga Draper, John Zupan, Douglas S. Conklin, and Patricia C. Zambryski. "New tools for protein linkage mapping and general two-hybrid screening." Yeast 15, no. 16 (1999): 1761–68. http://dx.doi.org/10.1002/(sici)1097-0061(199912)15:16<1761::aid-yea494>3.0.co;2-c.

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16

KAJITA, Shimpei, Kouyou AKIYAMA, Takehito TSUJI, and Tetsuo KUNIEDA. "Screening of TMEM48 binding proteins in testis by yeast two-hybrid method." Journal of Animal Genetics 41, no. 2 (2013): 77–86. http://dx.doi.org/10.5924/abgri.41.77.

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17

YANG, Sha, Yan LI, Feng GUO, et al. "Screening of AhCaM-Interactive Proteins in Peanuts Using Yeast Two Hybrid System." Acta Agronomica Sinica 41, no. 7 (2015): 1056. http://dx.doi.org/10.3724/sp.j.1006.2015.01056.

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18

Lievens, Sam, Maureen Caligiuri, Niko Kley, and Jan Tavernier. "The use of mammalian two-hybrid technologies for high-throughput drug screening." Methods 58, no. 4 (2012): 335–42. http://dx.doi.org/10.1016/j.ymeth.2012.08.003.

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19

Lu, Yin-Ying, Lin Wang, Jun Cheng, Ke Li, Yan Liu, and Ling-Xia Zhang. "Screening of HBcAg interacting proteins in hepatocytes with yeast-two hybrid technique." World Chinese Journal of Digestology 11, no. 4 (2003): 426–29. http://dx.doi.org/10.11569/wcjd.v11.i4.426.

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20

Saito, Youhei, Kazuya Doi, Nobuyuki Yamagishi, Keiichi Ishihara та Takumi Hatayama. "Screening of Hsp105α-binding proteins using yeast and bacterial two-hybrid systems". Biochemical and Biophysical Research Communications 314, № 2 (2004): 396–402. http://dx.doi.org/10.1016/j.bbrc.2003.12.108.

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21

Lin, Hsin-Ying, Sey-En Lin, Su-Fang Chien, and Ming-Kai Chern. "Electroporation for three commonly used yeast strains for two-hybrid screening experiments." Analytical Biochemistry 416, no. 1 (2011): 117–19. http://dx.doi.org/10.1016/j.ab.2011.04.038.

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22

Nallani, Karuna C., and William J. Sullivan. "Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening." Parasitology Research 95, no. 4 (2005): 236–42. http://dx.doi.org/10.1007/s00436-004-1291-5.

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23

KE, Dan-Xia, and Kun-Peng PENG. "Screening of NFR1α-interactive proteins in soybean using yeast two hybrid system." Acta Agronomica Sinica 46, no. 1 (2019): 31–39. http://dx.doi.org/10.3724/sp.j.1006.2020.94036.

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24

Tang, Keqin, Russell L. Finley,, Daotai Nie, and Kenneth V. Honn. "Identification of 12-Lipoxygenase Interaction with Cellular Proteins by Yeast Two-Hybrid Screening†." Biochemistry 39, no. 12 (2000): 3185–91. http://dx.doi.org/10.1021/bi992664v.

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25

Hoenicka, Janet, Montserrat Arrasate, Justo Garcia de Yebenes, and Jesús Avila. "A two-hybrid screening of human Tau protein: interactions with Alu-derived domain." Neuroreport 13, no. 3 (2002): 343–49. http://dx.doi.org/10.1097/00001756-200203040-00019.

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26

Matsumoto, D., and R. Tao. "YEAST TWO-HYBRID SCREENING FOR THE GENERAL INHIBITOR DETOXIFYING S-RNASE IN PRUNUS." Acta Horticulturae, no. 967 (November 2012): 167–70. http://dx.doi.org/10.17660/actahortic.2012.967.19.

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27

Li, Qiao, Pan Chen, Zhaoyang Zeng, et al. "Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2." Tumor Biology 37, no. 9 (2016): 12503–12. http://dx.doi.org/10.1007/s13277-016-5113-z.

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28

Holtgrawe, Daniela, Anke Scholz, Bianca Altmann, and Renate Scheibe. "Cytoskeleton-associated, carbohydrate-metabolizing enzymes in maize identified by yeast two-hybrid screening." Physiologia Plantarum 125, no. 2 (2005): 141–56. http://dx.doi.org/10.1111/j.1399-3054.2005.00548.x.

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29

Adyshev, Djanybek M., Irina A. Kolosova, and Alexander D. Verin. "Potential Protein Partners for the Human TIMAP Revealed by Bacterial Two-hybrid Screening." Molecular Biology Reports 33, no. 2 (2006): 83–89. http://dx.doi.org/10.1007/s11033-005-2311-y.

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30

Ma, W., S. Kilaru, C. Collins, M. Courbot, and G. Steinberg. "Libraries for two-hybrid screening of yeast and hyphal growth forms in Zymoseptoria tritici." Fungal Genetics and Biology 79 (June 2015): 94–101. http://dx.doi.org/10.1016/j.fgb.2015.03.023.

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31

Fiebitz, Andrea, Lajos Nyarsik, Bernard Haendler, et al. "High-throughput mammalian two-hybrid screening for protein-protein interactions using transfected cell arrays." BMC Genomics 9, no. 1 (2008): 68. http://dx.doi.org/10.1186/1471-2164-9-68.

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32

Ma, Qingyan, Hong Yu, Jijin Lin, Yifan Sun, Xinyuan Shen, and Li Ren. "Screening for cardiac HERG potassium channel interacting proteins using the yeast two‐hybrid technique." Cell Biology International 38, no. 2 (2013): 239–45. http://dx.doi.org/10.1002/cbin.10196.

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33

Erffelinck, Marie-Laure, Bianca Ribeiro, Maria Perassolo, et al. "A user-friendly platform for yeast two-hybrid library screening using next generation sequencing." PLOS ONE 13, no. 12 (2018): e0201270. http://dx.doi.org/10.1371/journal.pone.0201270.

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34

Kim, Hyun Jung, Kyung Hee Chong, Shin Wook Kang, et al. "Identification of cyclophilin A as a CD99-binding protein by yeast two-hybrid screening." Immunology Letters 95, no. 2 (2004): 155–59. http://dx.doi.org/10.1016/j.imlet.2004.07.001.

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35

Patrício, Daniela, and Margarida Fardilha. "The mammalian two-hybrid system as a powerful tool for high-throughput drug screening." Drug Discovery Today 25, no. 4 (2020): 764–71. http://dx.doi.org/10.1016/j.drudis.2020.01.022.

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36

Chen, Jun, Jianhong Zhou, Claire K. Sanders, John P. Nolan, and Hong Cai. "A surface display yeast two-hybrid screening system for high-throughput protein interactome mapping." Analytical Biochemistry 390, no. 1 (2009): 29–37. http://dx.doi.org/10.1016/j.ab.2009.03.013.

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37

Schenk, Jörg A., Stephan Heymann, Lars E. Peters, and Burkhard Micheel. "Screening for Recombinant Plasmids in Yeast Colonies of the Two-Hybrid System Using PCR." BioTechniques 20, no. 5 (1996): 812–16. http://dx.doi.org/10.2144/96205bm19.

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38

van Dijk, Kor-jent, Michelle Waycott, Joe Quarmby, et al. "Genomic Screening Reveals That the Endangered Eucalyptus paludicola (Myrtaceae) Is a Hybrid." Diversity 12, no. 12 (2020): 468. http://dx.doi.org/10.3390/d12120468.

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A hybrid origin for a conservation listed taxon will influence its status and management options. Here, we investigate the genetic origins of a nationally endangered listed taxon—Eucalyptus paludicola—a tree that is restricted to the Fleurieu Peninsula and Kangaroo Island of South Australia. Since its description in 1995, there have been suggestions that this taxon may potentially be a stable hybrid species. Using a high throughput sequencing approach, we developed a panel of polymorphic loci that were screened across E. paludicola and its putative parental species E. cosmophylla and E. ovata. Bayesian clustering of the genotype data identified separate groups comprising E. ovata and E. cosmophylla while E. paludicola individuals were admixed between these two, consistent with a hybrid origin. Hybrid class assignment tests indicate that the majority of E. paludicola individuals (~70%) are F1 hybrids with a low incidence of backcrossing. Most of the post-F1 hybrids were associated with revegetation sites suggesting they may be maladapted and rarely reach maturity under natural conditions. These data support the hypothesis that E. paludicola is a transient hybrid entity rather than a distinct hybrid species. We briefly discuss the conservation implications of our findings.
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39

Hastie, A. R., and S. C. Pruitt. "Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation." Nucleic Acids Research 35, no. 21 (2007): e141-e141. http://dx.doi.org/10.1093/nar/gkm894.

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40

Kim, Kyung-mi, Djanybek M. Adyshev, Anita Kása, et al. "Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening." Microvascular Research 88 (July 2013): 19–24. http://dx.doi.org/10.1016/j.mvr.2013.04.002.

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41

Ayers, Nancy A., Deborah A. Wilkinson, Thomas J. Fitzgerald та Gerald M. Carlson. "Self-association of the α Subunit of Phosphorylase Kinase as Determined by Two-hybrid Screening". Journal of Biological Chemistry 274, № 50 (1999): 35583–90. http://dx.doi.org/10.1074/jbc.274.50.35583.

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42

Zhang, Li-Ying, Jun Cheng, Hong Deng, Jiang Guo, Feng-Jin Guo, and Qiao-Xia Wang. "Screening of proteins binding to NS2TP from leukocyte cDNA library by yeast two-hybrid technique." World Chinese Journal of Digestology 13, no. 14 (2005): 1688. http://dx.doi.org/10.11569/wcjd.v13.i14.1688.

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43

Fu, Qing-Ying, та Yu-Qi Gao. "Screening of AMP-activated protein kinase α2 subunit interacting proteins by bacterial two-hybrid system". Molecular Biology Reports 36, № 2 (2007): 337–44. http://dx.doi.org/10.1007/s11033-007-9184-1.

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44

Xu, Teng-Fei, Jiang Xiang, Feng-Ju Li, et al. "Screening proteins interacting with VpPR10.1 of Chinese wild grapevine using the yeast two-hybrid system." Acta Physiologiae Plantarum 35, no. 8 (2013): 2355–64. http://dx.doi.org/10.1007/s11738-013-1269-y.

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45

Yoon, Jung-Min, Masatoshi Nakajima, Kiyoshi Mashiguchi, Seung-Hyun Park, Masato Otani, and Tadao Asami. "Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system." Bioorganic & Medicinal Chemistry Letters 23, no. 4 (2013): 1096–98. http://dx.doi.org/10.1016/j.bmcl.2012.12.007.

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46

Shahheydari, Hamideh, Sarah Frost, Brian J. Smith, Guy E. Groblewski, Yuyan Chen, and Jennifer A. Byrne. "Identification of PLP2 and RAB5C as novel TPD52 binding partners through yeast two-hybrid screening." Molecular Biology Reports 41, no. 7 (2014): 4565–72. http://dx.doi.org/10.1007/s11033-014-3327-y.

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47

Jeremy, M. Henley. "The yeast two-hybrid system: Screening for proteins which interaction with non-NMDA receptor subunits." Biochemical Society Transactions 25, no. 3 (1997): 536S. http://dx.doi.org/10.1042/bst025536s.

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48

Abdolmaleki, Azizeh, and Jahan B. Ghasemi. "Dual-acting of Hybrid Compounds - A New Dawn in the Discovery of Multi-target Drugs: Lead Generation Approaches." Current Topics in Medicinal Chemistry 17, no. 9 (2017): 1096–114. http://dx.doi.org/10.2174/1568026616666160927151144.

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Finding high quality beginning compounds is a critical job at the start of the lead generation stage for multi-target drug discovery (MTDD). Designing hybrid compounds as selective multitarget chemical entity is a challenge, opportunity, and new idea to better act against specific multiple targets. One hybrid molecule is formed by two (or more) pharmacophore group’s participation. So, these new compounds often exhibit two or more activities going about as multi-target drugs (mtdrugs) and may have superior safety or efficacy. Application of integrating a range of information and sophisticated new in silico, bioinformatics, structural biology, pharmacogenomics methods may be useful to discover/design, and synthesis of the new hybrid molecules. In this regard, many rational and screening approaches have followed by medicinal chemists for the lead generation in MTDD. Here, we review some popular lead generation approaches that have been used for designing multiple ligands (DMLs). This paper focuses on dual- acting chemical entities that incorporate a part of two drugs or bioactive compounds to compose hybrid molecules. Also, it presents some of key concepts and limitations/strengths of lead generation methods by comparing combination framework method with screening approaches. Besides, a number of examples to represent applications of hybrid molecules in the drug discovery are included.
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49

Beyazkilic, Pinar, Urandelger Tuvshindorj, Adem Yildirim, Caglar Elbuken, and Mehmet Bayindir. "Robust superhydrophilic patterning of superhydrophobic ormosil surfaces for high-throughput on-chip screening applications." RSC Advances 6, no. 83 (2016): 80049–54. http://dx.doi.org/10.1039/c6ra19669a.

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50

LeBoldus, Jared M., Peter V. Blenis, and Barb R. Thomas. "Clone by isolate interaction in the hybrid poplar – Septoria musiva pathosystem." Canadian Journal of Forest Research 38, no. 7 (2008): 1888–96. http://dx.doi.org/10.1139/x08-042.

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Fourteen clones of hybrid poplar were inoculated with 19 isolates of Septoria musiva Peck under greenhouse conditions to determine the magnitude of the clone, isolate, and clone × isolate interaction effects. Septoria musiva isolates were collected from five geographic areas, two symptoms (canker and leaf spot), and two host types (native species and hybrid poplar). The hybrid poplar clones were classified by parent type ( Populus deltoides Bartr. ex Marsh., Populus laurifolia Lebed. × Populus nigra L., and P. deltoides × (P. laurifolia × P. nigra)). There were no significant differences among geographic areas (p = 0.443), symptoms (p = 0.842), or hosts (p = 0.304) of origin for the 19 isolates nor significant differences among the three parent types (p = 0.089). Clone, isolate, and clone × isolate interaction effects were all significant, accounting for 65%, 15%, and 18%, respectively, of the explained variation. These results indicate that clones rather than parent types should be the focus of resistance screening programs and that the pathosystem should be stable given the relatively small clone × isolate interaction. These results also indicate that a single isolate should be sufficient for preliminary screening of disease resistance in hybrid poplars.
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