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Journal articles on the topic 'Two time lapse video microscopy'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Peippo, Jaana, and Peter Bredbacka. "A simple culture system for time-lapse video recording of bovine embryos." Agricultural and Food Science 5, no. 5 (1996): 515–20. http://dx.doi.org/10.23986/afsci.72763.

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Continuous observation of embryonic growth can improve understanding of the early developmental events and allow us to use parametric statistical analyses with time as a parameter. A cinematographic study such as that reported here utilizes time-lapse video recording. Previously published methods for time-lapse video recording have involved building an incubator around a microscope, a process that is both expensive and laborious. Here we present a simplified method for time-lapse video recording of early bovine embryo development. The embryos were cultured during a 24-hour period in a standard

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2

Straight, Aaron F., John W. Sedat, and Andrew W. Murray. "Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast." Journal of Cell Biology 143, no. 3 (1998): 687–94. http://dx.doi.org/10.1083/jcb.143.3.687.

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The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three of these motors by video microscopy of motor mutants whose microtubules and centromeres were marked with green fluorescent protein. Despite their functional overlap, each motor mutant has a specific defect in mitosis: cin8Δ mutants lack the rapid phase of anaphase B, kip1Δ mutants show defects in the slow phase of anaphase B, and kip3Δ mutants

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3

Yuan, Liang, and Junda Zhu. "Video Object Tracking in Neural Axons with Fluorescence Microscopy Images." Journal of Applied Mathematics 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/423876.

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Neurofilament is an important type of intercellular cargos transmitted in neural axons. Given fluorescence microscopy images, existing methods extract neurofilament movement patterns by manual tracking. In this paper, we describe two automated tracking methods for analyzing neurofilament movement based on two different techniques: constrained particle filtering and tracking-by-detection. First, we introduce the constrained particle filtering approach. In this approach, the orientation and position of a particle are constrained by the axon’s shape such that fewer particles are necessary for tra

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4

Phillipson, Mia, Bryan Heit, Pina Colarusso, Lixin Liu, Christie M. Ballantyne, and Paul Kubes. "Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade." Journal of Experimental Medicine 203, no. 12 (2006): 2569–75. http://dx.doi.org/10.1084/jem.20060925.

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The prevailing view is that the β2-integrins Mac-1 (αMβ2, CD11b/CD18) and LFA-1 (αLβ2, CD11a/CD18) serve similar biological functions, namely adhesion, in the leukocyte recruitment cascade. Using real-time and time-lapse intravital video-microscopy and confocal microscopy within inflamed microvessels, we systematically evaluated the function of Mac-1 and LFA-1 in the recruitment paradigm. The chemokine macrophage inflammatory protein-2 induced equivalent amounts of adhesion in wild-type and Mac-1−/− mice but very little adhesion in LFA-1−/− mice. Time-lapse video-microscopy within the postcapi

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Bell, Paul B., Margaretha Lindroth, Karin Hedberg, Bengt-Arne Fredriksson, and Åke Wasteson. "Reorganization of the Actin Cytoskeleton in Human Fibroblasts Induced by PDGF and Neomycin." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 180–81. http://dx.doi.org/10.1017/s0424820100158443.

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Among the earliest effects on cells of platelet derived growth factor (PDGF), a growth-stimulating factor normally occurring in blood, are stimulation of lamellipodia formation and induction of changes in the actin cytoskeleton. We have investigated these phenomena by time lapse video (TLV) and fluorescence light microscopy (FLM), and high resolution scanning electron microscopy (SEM) using two strains of human diploid fibroblasts: ICIG-7 from embryonic lung and AG1523 from foreskin, grown either on coverglasses or on carbon-stabilized Formvar-coated gold electron microscope grids. Cells were

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6

Cole, Richard W., Alexey Khodjakov, William H. Wright, and Conly L. Rieder. "A Differential Interference Contrast-Based Light Microscopic System for Laser Microsurgery and Optical Trapping of Selected Chromosomes during Mitosis In Vivo." Microscopy and Microanalysis 1, no. 5 (1995): 203–15. http://dx.doi.org/10.1017/s1431927695112039.

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Laser microsurgery and laser-generated optical force traps (optical tweezers) are both valuable light microscopic-based approaches for studying intra- and extracellular motility processes, including chromosome segregation during mitosis. Here we describe a system in use in our laboratory that allows living cells to be followed by high-resolution differential interference contrast (DIC) video-enhanced time-lapse light microscopy while selected mitotic organelles and spindle components are subjected to laser microsurgery and/or manipulation with an optical force trap. This system couples the out

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Sanderson, Christopher M., Michael Way, and Geoffrey L. Smith. "Virus-Induced Cell Motility." Journal of Virology 72, no. 2 (1998): 1235–43. http://dx.doi.org/10.1128/jvi.72.2.1235-1243.1998.

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ABSTRACT Many viruses induce profound changes in cell metabolism and function. Here we show that vaccinia virus induces two distinct forms of cell movement. Virus-induced cell migration was demonstrated by an in vitro wound healing assay in which infected cells migrated independently into the wound area while uninfected cells remained relatively static. Time-lapse microscopy showed that the maximal rate of migration occurred between 9 and 12 h postinfection. Virus-induced cell migration was inhibited by preinactivation of viral particles with trioxsalen and UV light or by the addition of cyclo

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8

Swanson, J. A., M. T. Johnson, K. Beningo, P. Post, M. Mooseker, and N. Araki. "A contractile activity that closes phagosomes in macrophages." Journal of Cell Science 112, no. 3 (1999): 307–16. http://dx.doi.org/10.1242/jcs.112.3.307.

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Studies of Fc-mediated phagocytosis by mouse macrophages identified a contractile activity at the distal margins of forming phagosomes. Time-lapse video microscopic analysis of macrophages containing rhodamine-labeled actin and fluorescein dextran showed that actin was concentrated at the distal margins of closing phagosomes. Phagocytosis-related contractile activities were observed when one IgG-opsonized erythrocyte was engaged by two macrophages. Both cells extended pseudopodia until they met midway around the erythrocyte. It was then constricted and pulled into two phagosomes, which remaine

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9

Laster, S. M., J. G. Wood, and L. R. Gooding. "Target-induced changes in macrophage migration may explain differences in lytic sensitivity among simian virus 40-transformed fibroblasts." Journal of Immunology 141, no. 1 (1988): 221–27. http://dx.doi.org/10.4049/jimmunol.141.1.221.

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Abstract Time-lapse video microscopy has been used to study macrophage movement in the presence of SV40-transformed fibroblasts. In all, five different SV40-transformed cell lines (an uncloned line and four clones derived from it) were tested for their affects on the movement of LPS-activated macrophages (AM). Conditions for video microscope recording were designed to simulate, as best as possible, the conditions used in a 51Cr-release cytotoxicity assay. Our analysis shows that these targets had a range of effects on macrophage migration, from stimulation to complete inhibition of movement. T

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Qian, X., S. K. Goderie, Q. Shen, J. H. Stern, and S. Temple. "Intrinsic programs of patterned cell lineages in isolated vertebrate CNS ventricular zone cells." Development 125, no. 16 (1998): 3143–52. http://dx.doi.org/10.1242/dev.125.16.3143.

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Using long-term, time-lapse video-microscopy, we investigated how single progenitor cells isolated from the early embryonic cerebral cortex produce neurons and glia over time. Clones of 10 cells or less were produced by short symmetric or asymmetric division patterns, commonly terminating in a ‘pair progenitor’ for two morphologically identical neurons. Larger trees were composites of these short sub-lineages: more prolific neuroblasts underwent repeated asymmetric divisions, each producing a minor neuroblast that typically made (3/4)10 progeny, and a sister cell capable of generating more pro

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Roberts, R. L., M. A. Barbieri, and P. D. Stahl. "Endosome Fusion and Tubule Formation in Cells Overexpressing GFP-Rab5 Fusion Proteins." Microscopy and Microanalysis 3, S2 (1997): 137–38. http://dx.doi.org/10.1017/s1431927600007571.

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Previous studies have demonstrated that GTPgS and rab5:Q79L, a constitutively active rab5 mutant, stimulate endosome fusion in in vitro assays [Cell Reg. 1:113-124 (1989)]. Additionally, electron microscopy has shown that under these conditions, endosomes form incompletely fused couplets [Cell Reg. 1:113-124 (1989); Arch. Biochem. Biophys. 326:64-72 (1996)]. CHO and BHK cells that overexpress rab5:Q79L develop ‘giant’ cytoplasmic vesicles that exhibit many characteristics of early endosomes including immunoreactivity for rab 5 and transferrin receptor (data not shown). The formation of these v

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Jacquet, Michel, Georges Renault, Sylvie Lallet, Jan De Mey, and Albert Goldbeter. "Oscillatory nucleocytoplasmic shuttling of the general stress response transcriptional activators Msn2 and Msn4 in Saccharomyces cerevisiae." Journal of Cell Biology 161, no. 3 (2003): 497–505. http://dx.doi.org/10.1083/jcb.200303030.

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Msn2 and Msn4 are two related transcriptional activators that mediate a general response to stress in yeast Saccharomyces cerevisiae by eliciting the expression of specific sets of genes. In response to stress or nutritional limitation, Msn2 and Msn4 migrate from the cytoplasm to the nucleus. Using GFP-tagged constructs and high-resolution time-lapse video microscopy on single cells, we show that light emitted by the microscope also triggers this migration. Unexpectedly, the population of Msn2 or Msn4 molecules shuttles repetitively into and out of the nucleus with a periodicity of a few minut

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13

Keller, R. E., Michael Danilchik, Robert Gimlich, and John Shih. "The function and mechanism of convergent extension during gastrulation of Xenopus laevis." Development 89, Supplement (1985): 185–209. http://dx.doi.org/10.1242/dev.89.supplement.185.

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The processes thought to function in Xenopus gastrulation include bottle cell formation, migration of cells on the roof of the blastocoel, and autonomous convergent extension of the circumblastoporal region. A review of recent and classical results shows that only the last accounts for the bulk of the tissue displacement of gastrulation, including spreading of the marginal zone toward the blastopore, involution of the marginal zone, and closure of the blastopore. Microsurgical manipulation and explantation studies, analysed by time-lapse video and cine microscopy, shows that the dorsal circumb

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14

Thomas, L. A., and K. M. Yamada. "Contact stimulation of cell migration." Journal of Cell Science 103, no. 4 (1992): 1211–14. http://dx.doi.org/10.1242/jcs.103.4.1211.

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Mass migrations of dense cell populations occur periodically during embryonic development. It is known that extracellular matrices, through which the cells migrate, facilitate locomotion. However, this does not explain how cells, such as neural crest, can migrate as a dense cohort of cells in essentially continuous contact with one another. We report here that unique behavioral characteristics of the migrating cells may contribute to cohesive migration. We used time-lapse video microscopy to analyze the migration of quail neural crest cells and of two crest derivatives, human melanoma cells an

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15

Cramer, L. P., and T. J. Mitchison. "Investigation of the mechanism of retraction of the cell margin and rearward flow of nodules during mitotic cell rounding." Molecular Biology of the Cell 8, no. 1 (1997): 109–19. http://dx.doi.org/10.1091/mbc.8.1.109.

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We have studied two types of cell motility directed toward the cell center: retraction of the cell margin and rearward flow of small cytoplasmic nodules during mitotic cell rounding in Potoroo tridactylis kidney (PtK2) cells by time-lapse video microscopy, drug treatments, and photoactivation of fluorescence. Nodules flow rearward on thin, actin-rich fibers (retraction fibers) exposed as the cell margin retracts. Retraction of the cell margin and rearward flow of nodules require intact actin filaments, but are insensitive to an inhibitor of myosin function (butanedione monoxime). Using photoac

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16

Hu, Ke, Tara Mann, Boris Striepen, Con J. M. Beckers, David S. Roos, and John M. Murray. "Daughter Cell Assembly in the Protozoan ParasiteToxoplasma gondii." Molecular Biology of the Cell 13, no. 2 (2002): 593–606. http://dx.doi.org/10.1091/mbc.01-06-0309.

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The phylum Apicomplexa includes thousands of species of obligate intracellular parasites, many of which are significant human and/or animal pathogens. Parasites in this phylum replicate by assembling daughters within the mother, using a cytoskeletal and membranous scaffolding termed the inner membrane complex. Most apicomplexan parasites, including Plasmodium sp. (which cause malaria), package many daughters within a single mother during mitosis, whereas Toxoplasma gondii typically packages only two. The comparatively simple pattern of T. gondii cell division, combined with its molecular genet

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17

Gönczy, Pierre, Heinke Schnabel, Titus Kaletta, Ana Duran Amores, Tony Hyman, and Ralf Schnabel. "Dissection of Cell Division Processes in the One Cell Stage Caenorhabditis elegans Embryo by Mutational Analysis." Journal of Cell Biology 144, no. 5 (1999): 927–46. http://dx.doi.org/10.1083/jcb.144.5.927.

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To identify novel components required for cell division processes in complex eukaryotes, we have undertaken an extensive mutational analysis in the one cell stage Caenorhabditis elegans embryo. The large size and optical properties of this cell permit observation of cell division processes with great detail in live specimens by simple differential interference contrast (DIC) microscopy. We have screened an extensive collection of maternal-effect embryonic lethal mutations on chromosome III with time-lapse DIC video microscopy. Using this assay, we have identified 48 mutations in 34 loci which

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18

Leblanc-Latour, Maxime, Craig Bryan, and Andrew E. Pelling. "Utilizing social media and video games to control #DIY microscopes." PeerJ Computer Science 3 (December 11, 2017): e139. http://dx.doi.org/10.7717/peerj-cs.139.

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Open-source lab equipment is becoming more widespread with the popularization of fabrication tools such as 3D printers, laser cutters, CNC machines, open source microcontrollers and open source software. Although many pieces of common laboratory equipment have been developed, software control of these items is sometimes lacking. Specifically, control software that can be easily implemented and enable user-input and control over multiple platforms (PC, smartphone, web, etc.). The aim of this proof-of principle study was to develop and implement software for the control of a low-cost, 3D printed

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19

Luparello, C., P. Sheterline, I. Pucci-Minafra, and S. Minafra. "A comparison of spreading and motility behaviour of 8701-BC breast carcinoma cells on type I, I-trimer and type V collagen substrata. Evidence for a permissive effect of type I-trimer collagen on cell locomotion." Journal of Cell Science 100, no. 1 (1991): 179–85. http://dx.doi.org/10.1242/jcs.100.1.179.

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Ductal infiltrating carcinoma (d.i.c.) of human breast is a highly invasive neoplasm characterized by enhanced deposition of collagen. Paradoxically, enhanced collagen deposition is not correlated with inhibition of the migration of tumour cells into the host tissue. d.i.c. is characterized by the reappearance of ‘embryonic’ type I-trimer collagen and an increase in type V collagen content in the matrix. The effects of these two collagen types were compared with type I collagen as culture substrata on the spreading pattern, cytoskeletal organization and motile behaviour of 8701-BC breast carci

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Hoepfner, Dominic, Marlene van den Berg, Peter Philippsen, Henk F. Tabak, and Ewald H. Hettema. "A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae." Journal of Cell Biology 155, no. 6 (2001): 979–90. http://dx.doi.org/10.1083/jcb.200107028.

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In vivo time-lapse microscopy reveals that the number of peroxisomes in Saccharomyces cerevisiae cells is fairly constant and that a subset of the organelles are targeted and segregated to the bud in a highly ordered, vectorial process. The dynamin-like protein Vps1p controls the number of peroxisomes, since in a vps1Δ mutant only one or two giant peroxisomes remain. Analogous to the function of other dynamin-related proteins, Vps1p may be involved in a membrane fission event that is required for the regulation of peroxisome abundance. We found that efficient segregation of peroxisomes from mo

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Irvine, K. D., and E. Wieschaus. "Cell intercalation during Drosophila germband extension and its regulation by pair-rule segmentation genes." Development 120, no. 4 (1994): 827–41. http://dx.doi.org/10.1242/dev.120.4.827.

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After the onset of gastrulation, the Drosophila germband undergoes a morphological change in which its length along the anterior-posterior axis increases over two-and-a-half fold while its width along the dorsal-ventral axis simultaneously narrows. The behavior of individual cells during germband extension was investigated by epi-illumination and time-lapse video microscopy of living embryos. Cells intercalate between their dorsal and ventral neighbors during extension, increasing the number of cells along the anterior-posterior axis while decreasing the number of cells along the dorsal-ventra

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Rodríguez-Romero, Adela, Alejandra Hernández-Santoyo, Deyanira Fuentes-Silva та ін. "Structural analysis of the endogenous glycoallergen Hev b 2 (endo-β-1,3-glucanase) fromHevea brasiliensisand its recognition by human basophils". Acta Crystallographica Section D Biological Crystallography 70, № 2 (2014): 329–41. http://dx.doi.org/10.1107/s1399004713027673.

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Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) fromHevea brasiliensisis an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope that could be responsible for its cross-reactivity. Here, the structure of the endogenous isoform II of Hev b 2 that exhibits three post-translational modifications, including an N-terminal pyroglutamate and two glycosylation sites at Asn27 and at Asn314, is reported from two crystal polymorphs. These modifications f

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Bement, W. M., P. Forscher, and M. S. Mooseker. "A novel cytoskeletal structure involved in purse string wound closure and cell polarity maintenance." Journal of Cell Biology 121, no. 3 (1993): 565–78. http://dx.doi.org/10.1083/jcb.121.3.565.

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The process of wound repair in monolayers of the intestinal epithelial cell line, Caco-2BBe, was analyzed by a combination of time-lapse differential interference contrast (DIC) video and immunofluorescence microscopy, and laser scanning confocal immunofluorescence microscopy (LSCIM). DIC video analysis revealed that stab wounds made in Caco-2BBe monolayers healed by two distinct processes: (a) Extension of lamellipodia into the wounds; and (b) Purse string closure of the wound by distinct arcs or rings formed by cells bordering the wound. The arcs and rings which effected purse string closure

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Giacobini, P., C. Giampietro, M. Fioretto, et al. "Hepatocyte Growth Factor/Scatter Factor Facilitates Migration of GN-11 Immortalized LHRH Neurons." Endocrinology 143, no. 9 (2002): 3306–15. http://dx.doi.org/10.1210/en.2002-220146.

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Abstract The molecular cues regulating the migratory process of LHRH neurons from the olfactory placode into the brain are not well known, but gradients of chemotropic and chemorepellent factors secreted by the targets are likely to play a key role in guidance mechanisms. Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine inducing cell migration. It is involved in a variety of developmental processes through interaction with its receptor c-Met. Here we show that c-Met-antibody labels LHRH migrating neurons in the olfactory mesenchyme of E12 mouse and analyze the potenti

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Siller, Karsten H., Madeline Serr, Ruth Steward, Tom S. Hays, and Chris Q. Doe. "Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for Lis1/Dynactin in Spindle Assembly and Mitotic Checkpoint Control." Molecular Biology of the Cell 16, no. 11 (2005): 5127–40. http://dx.doi.org/10.1091/mbc.e05-04-0338.

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Lis1 is required for nuclear migration in fungi, cell cycle progression in mammals, and the formation of a folded cerebral cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactin-dependent processes is not clearly understood. Here we generate and/or characterize mutants for Drosophila Lis1 and a dynactin subunit, Glued, to investigate the role of Lis1/dynactin in mitotic checkpoint function. In addition, we develop an improved time-lapse video microscopy technique that allows live imaging of GFP-Lis1, GFP-Rod checkpoint protein, green fl

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Schor, A. M., T. D. Allen, A. E. Canfield, P. Sloan, and S. L. Schor. "Pericytes derived from the retinal microvasculature undergo calcification in vitro." Journal of Cell Science 97, no. 3 (1990): 449–61. http://dx.doi.org/10.1242/jcs.97.3.449.

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Pericytes isolated from the bovine retinal microvasculature retain characteristic features of their in vivo counterparts, such as the presence of glycogen deposits, long filamentous processes, prominent microfilament bundles and the ability to display two distinct and reversible phenotypes. Time-lapse video-microscopy demonstrated that pericytes tend to overlap and aggregate, even in sparse cultures. After reaching confluence, they form multilayered areas that retract away from each other, resulting in the formation of multicellular nodules. These nodules increase in size and cellularity by go

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Rovensky, Y. A., L. V. Domnina, O. Y. Ivanova, and J. M. Vasiliev. "Locomotory behaviour of epitheliocytes and fibroblasts on metallic grids." Journal of Cell Science 112, no. 8 (1999): 1273–82. http://dx.doi.org/10.1242/jcs.112.8.1273.

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Behaviour of epitheliocytes and fibroblasts on special discontinuous substrata (metallic grids with square openings of 45x45 microm2) was examined in order to compare the ability of these cells to spread in two mutually perpendicular directions and to stretch over the void spaces. Two cell types with typical fibroblastic morphology, the AGO 1523 line of human foreskin fibroblasts and secondary cultures of mouse embryo fibroblasts, and three cell types with typical epithelial morphology, primary mouse hepatocytes, the IAR-2 line of rat liver cells and the MDCK line of canine kidney epithelial c

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Eliott, S., G. H. Joss, A. Spudich, and K. L. Williams. "Patterns in Dictyostelium discoideum: the role of myosin II in the transition from the unicellular to the multicellular phase." Journal of Cell Science 104, no. 2 (1993): 457–66. http://dx.doi.org/10.1242/jcs.104.2.457.

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Dictyostelium discoideum amoebae which lack the myosin II gene are motile and aggregate to form rudimentary mounds, but do not undergo further morphological development (Manstein et al., 1989). Here we use scanning electron microscopy, light microscopy, immunofluorescence and computer analysis of time-lapse video films to study how D. discoideum myosin null cells of strains HS2205 and HS2206 aggregate. Myosin null cells are sufficiently coordinated in their movements to form two-dimensional aggregation streams, although mutant cells within streams lack the elongated shape and parallel orientat

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Yáñez-Mó, María, Arántzazu Alfranca, Carlos Cabañas та ін. "Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with α3β1 Integrin Localized at Endothelial Lateral Junctions". Journal of Cell Biology 141, № 3 (1998): 791–804. http://dx.doi.org/10.1083/jcb.141.3.791.

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Cell-to-cell junction structures play a key role in cell growth rate control and cell polarization. In endothelial cells (EC), these structures are also involved in regulation of vascular permeability and leukocyte extravasation. To identify novel components in EC intercellular junctions, mAbs against these cells were produced and selected using a morphological screening by immunofluorescence microscopy. Two novel mAbs, LIA1/1 and VJ1/16, specifically recognized a 25-kD protein that was selectively localized at cell–cell junctions of EC, both in the primary formation of cell monolayers and whe

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Alfano, Alberto, Sergio D’ambrosio, Antonella D’Agostino, Rosario Finamore, Chiara Schiraldi, and Donatella Cimini. "Concentrated Buffalo Whey as Substrate for Probiotic Cultures and as Source of Bioactive Ingredients: A Local Circular Economy Approach towards Reuse of Wastewaters." Fermentation 7, no. 4 (2021): 281. http://dx.doi.org/10.3390/fermentation7040281.

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Waste reduction and reuse is a crucial target of current research efforts. In this respect, the present study was focused on providing an example of local investment in a simple process configuration that converts whey into value-added compounds and allows recovery of a clean water stream. In particular, buffalo milk whey obtained during mozzarella manufacturing was ultrafiltered in-house on spiral membrane modules (20 kDa), and the two obtained fractions, namely the retentate and the permeate, provided by the dairy factory, were further processed during this work. The use of an additional nan

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Simm, A., G. Bertsch, H. Frank, U. Zimmermann, and J. Hoppe. "Cell death of AKR-2B fibroblasts after serum removal: a process between apoptosis and necrosis." Journal of Cell Science 110, no. 7 (1997): 819–28. http://dx.doi.org/10.1242/jcs.110.7.819.

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AKR-2B cells disintegrate after serum removal. After a delay of approximately 90 minutes, cell death began and reached after six hours a plateau of 40–50% remaining living cells. We used time-lapse video microscopy to monitor dynamic structural changes and to measure the time span of individual cells to die. The first change was the rapid appearance of membrane blebs. Membrane vesicles were rapidly extruded and reintegrated by the cell. This highly dynamic process of an affected cell stopped after 80+/−20 minutes with its death. Conductivity measurements showed that at that time the membrane w

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Engqvist-Goldstein, Åsa E. Y., Michael M. Kessels, Vikramjit S. Chopra, Michael R. Hayden, and David G. Drubin. "An Actin-Binding Protein of the Sla2/Huntingtin Interacting Protein 1 Family Is a Novel Component of Clathrin-Coated Pits and Vesicles." Journal of Cell Biology 147, no. 7 (1999): 1503–18. http://dx.doi.org/10.1083/jcb.147.7.1503.

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The actin cytoskeleton has been implicated in endocytosis, yet few molecules that link these systems have been identified. Here, we have cloned and characterized mHip1R, a protein that is closely related to huntingtin interacting protein 1 (Hip1). These two proteins are mammalian homologues of Sla2p, an actin binding protein important for actin organization and endocytosis in yeast. Sequence alignments and secondary structure predictions verified that mHip1R belongs to the Sla2 protein family. Thus, mHip1R contains an NH2-terminal domain homologous to that implicated in Sla2p's endocytic funct

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Mitchell, Gabriel, Mandy I. Cheng, Chen Chen, et al. "Listeria monocytogenes triggers noncanonical autophagy upon phagocytosis, but avoids subsequent growth-restricting xenophagy." Proceedings of the National Academy of Sciences 115, no. 2 (2017): E210—E217. http://dx.doi.org/10.1073/pnas.1716055115.

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Xenophagy is a selective macroautophagic process that protects the host cytosol by entrapping and delivering microbes to a degradative compartment. Both noncanonical autophagic pathways and xenophagy are activated by microbes during infection, but the relative importance and function of these distinct processes are not clear. In this study, we used bacterial and host mutants to dissect the contribution of autophagic processes responsible for bacterial growth restriction of Listeria monocytogenes. L. monocytogenes is a facultative intracellular pathogen that escapes from phagosomes, grows in th

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Woo, K., and S. E. Fraser. "Order and coherence in the fate map of the zebrafish nervous system." Development 121, no. 8 (1995): 2595–609. http://dx.doi.org/10.1242/dev.121.8.2595.

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The zebrafish is an excellent vertebrate model for the study of the cellular interactions underlying the patterning and the morphogenesis of the nervous system. Here, we report regional fate maps of the zebrafish anterior nervous system at two key stages of neural development: the beginning (6 hours) and the end (10 hours) of gastrulation. Early in gastrulation, we find that the presumptive neurectoderm displays a predictable organization that reflects the future anteroposterior and dorsoventral order of the central nervous system. The precursors of the major brain subdivisions (forebrain, mid

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Laster, S. M., J. G. Wood, and L. R. Gooding. "Tumor necrosis factor can induce both apoptic and necrotic forms of cell lysis." Journal of Immunology 141, no. 8 (1988): 2629–34. http://dx.doi.org/10.4049/jimmunol.141.8.2629.

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Abstract TNF is a protein toxin which is secreted by activated macrophages and monocytes. Although the cytotoxic activity of TNF has been well documented, the mechanism of TNF-induced lysis is not well understood. The goal of this investigation was to determine whether TNF caused one of the classic forms of cell death, i.e., apoptosis, which is characterized by nuclear disintegration and cytoplasmic "boiling," or necrosis, which is characterized by the formation of a "balloon-like" plasma membrane and a lack of nuclear disintegration. Therefore, to distinguish apoptosis from necrosis, we have

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Ward, Brian M., and Bernard Moss. "Vaccinia Virus Intracellular Movement Is Associated with Microtubules and Independent of Actin Tails." Journal of Virology 75, no. 23 (2001): 11651–63. http://dx.doi.org/10.1128/jvi.75.23.11651-11663.2001.

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ABSTRACT Two mechanisms have been proposed for the intracellular movement of enveloped vaccinia virus virions: rapid actin polymerization and microtubule association. The first mechanism is used by the intracellular pathogens Listeria andShigella, and the second is used by cellular vesicles transiting from the Golgi network to the plasma membrane. To distinguish between these models, two recombinant vaccinia viruses that express the B5R membrane protein fused to enhanced green fluorescent protein (GFP) were constructed. One had Tyr112 and Tyr132 of the A36R membrane protein, which are required

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Brown, A. F., and G. A. Dunn. "Microinterferometry of the movement of dry matter in fibroblasts." Journal of Cell Science 92, no. 3 (1989): 379–89. http://dx.doi.org/10.1242/jcs.92.3.379.

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We describe the use of interferometric microscopy coupled with a novel application of Senarmont compensation for detecting and quantifying the distribution of dry matter in cultured cells. In conjunction with video techniques and digital image processing, a two-dimensional, calibrated map of the dry mass distribution in an isolated cell can be obtained and digitally recorded. We have called the technique Digitally Recorded Interferometric Microscopy with Analyser Shift (DRIMAS). The method greatly facilitates the automatic recognition of cells by computer. Recorded time-lapse sequences can be

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Rivero, F., B. Koppel, B. Peracino, et al. "The role of the cortical cytoskeleton: F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development." Journal of Cell Science 109, no. 11 (1996): 2679–91. http://dx.doi.org/10.1242/jcs.109.11.2679.

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We generated Dictyostelium double mutants lacking the two F-actin crosslinking proteins alpha-actinin and gelation factor by inactivating the corresponding genes via homologous recombination. Here we investigated the consequences of these deficiencies both at the single cell level and at the multicellular stage. We found that loss of both proteins severely affected growth of the mutant cells in shaking suspension, and led to a reduction of cell size from 12 microns in wild-type cells to 9 microns in mutant cells. Moreover the cells did not exhibit the typical polarized morphology of aggregatin

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Carron, CP, DM Meyer, VW Engleman, et al. "Peptidomimetic antagonists of alphavbeta3 inhibit bone resorption by inhibiting osteoclast bone resorptive activity, not osteoclast adhesion to bone." Journal of Endocrinology 165, no. 3 (2000): 587–98. http://dx.doi.org/10.1677/joe.0.1650587.

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Osteoclasts are actively motile on bone surfaces and undergo alternating cycles of migration and resorption. Osteoclast interaction with the extracellular matrix plays a key role in the osteoclast resorptive process and a substantial body of evidence suggests that integrin receptors are important in osteoclast function. These integrin receptors bind to the Arg-Gly-Asp (RGD) sequence found in a variety of extracellular matrix proteins and it is well established that the interaction of osteoclast alpha v beta 3 integrin with the RGD motif within bone matrix proteins is important in osteoclast-me

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Rønnov-Jessen, L., and O. W. Petersen. "A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts." Journal of Cell Biology 134, no. 1 (1996): 67–80. http://dx.doi.org/10.1083/jcb.134.1.67.

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Actins are known to comprise six mammalian isoforms of which beta- and gamma-nonmuscle actins are present in all cells, whereas alpha-smooth muscle (alpha-sm) actin is normally restricted to cells of the smooth muscle lineages. alpha-Sm actin has been found also to be expressed transiently in certain nonmuscle cells, in particular fibroblasts, which are referred to as myofibroblasts. The functional significance of alpha-sm actin in fibroblasts is unknown. However, myofibroblasts appear to play a prominent role in stromal reaction in breast cancer, at the site of wound repair, and in fibrotic r

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Patel, Jaimin M., Elizabeth Sapey, Dhruv Parekh, et al. "Sepsis Induces a Dysregulated Neutrophil Phenotype That Is Associated with Increased Mortality." Mediators of Inflammation 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/4065362.

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Background. Neutrophil dysfunction in sepsis has been implicated in the pathogenesis of multiorgan failure; however, the role of neutrophil extracellular traps (NETs) remains uncertain. We aimed to determine the sequential changes in ex vivo NETosis and its relationship with mortality in patients with sepsis and severe sepsis. Methods. This was a prospective observational cohort study enrolling 21 healthy age-matched controls and 39 sepsis and 60 severe sepsis patients from acute admissions to two UK hospitals. Patients had sequential bloods for the ex vivo assessment of NETosis in response to

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Nys, B., A. Van Daele, and W. Jacob. "An automatic time lapse video system for optical microscopy." Micron and Microscopica Acta 22, no. 3 (1991): 275–76. http://dx.doi.org/10.1016/0739-6260(91)90019-v.

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Allen, T. D. "Time lapse video microscopy using an animation control unit." Journal of Microscopy 147, no. 2 (1987): 129–35. http://dx.doi.org/10.1111/j.1365-2818.1987.tb02825.x.

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WANG, HELEN, J. BOXALL, H. HELLQUIST, D. PROOPS, and L. MICHAELS. "Video time-lapse microscopy of human laryngeal carcinomas in vitro." Clinical Otolaryngology 11, no. 5 (1986): 337–43. http://dx.doi.org/10.1111/j.1365-2273.1986.tb00135.x.

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WANG, HELEN, J. BOXALL, H. HELLQUIST, D. PROOPS, and L. MICHAELS. "Video time-lapse microscopy of human laryngeal carcinomas in vitro." Clinical Otolaryngology & Allied Sciences 11, no. 5 (2009): 337–43. http://dx.doi.org/10.1111/j.1365-2273.1986.tb02021.x.

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Di Giuseppe, Dario, Sonia Scarfì, Andrea Alessandrini, et al. "Acute cytotoxicity of mineral fibres observed by time-lapse video microscopy." Toxicology 466 (January 2022): 153081. http://dx.doi.org/10.1016/j.tox.2021.153081.

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Asada, Masahito, Yasuyuki Goto, Kazuhide Yahata, et al. "Gliding Motility of Babesia bovis Merozoites Visualized by Time-Lapse Video Microscopy." PLoS ONE 7, no. 4 (2012): e35227. http://dx.doi.org/10.1371/journal.pone.0035227.

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Casoni, Filippo, Michele Fornaro, and Susan Wray. "Dynamics of GnRH-1 neuronal migration: A time-lapse video microscopy study." Frontiers in Neuroendocrinology 27, no. 1 (2006): 158. http://dx.doi.org/10.1016/j.yfrne.2006.03.337.

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He, Ruian, Ri Cheng, Xinkai Lyu, Weimin Tan, and Bo Yan. "Efficient Online Training for Zero-Shot Time-Lapse Microscopy Denoising and Super-Resolution." Proceedings of the AAAI Conference on Artificial Intelligence 39, no. 3 (2025): 3419–27. https://doi.org/10.1609/aaai.v39i3.32354.

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In time-lapse microscopy, inherent noise significantly limits imaging sensitivity and increases measurement uncertainty. Due to the scarcity of clean data, zero-shot approaches have emerged as highly data-efficient solutions for microscopy denoising. However, existing methods typically process video frames independently, resulting in long training times and issues such as temporal noise and over-smoothing. In this paper, we introduce MDSR-Zero, a zero-shot online learning method designed for plug-and-play noise suppression and super-resolution of microscopy videos. Our approach leverages an ef

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HERLAMBANG, RUDY W., MOHAMAD SUHARTO, TRI MARUTAMA, and NADIA SIGI PRAMESWARI. "AESTHETIC VALUES ON TIME LAPSE AND CINEMATIC VIDEOS." International Journal of Creative Future and Heritage (TENIAT) 7, no. 2 (2019): 1–15. http://dx.doi.org/10.47252/teniat.v7i2.193.

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Perkembangan teknologi maklumat semakin mendorong manusia untuk membangun dan mencipta inovasi. Teknologi dapat mengembangkan potensi manusia dalam mencipta produk moden. Transformasi teknologi yang pesat dari semasa ke semasa dengan meluasnya perkembangan media audiovisual, serta teknik dalam penggambaran video seperti selang masa dan sinematik. Kajian ini bertujuan untuk mengkaji nilai estetika teknik video selang masa dan sinematik dengan mengambil kajian kes dua video, iaitu Exploring Cultural Heritage: The Old Wall in Solo and The Beauty of the City of Solo (Surakarta). Analisis dilakukan

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