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Relevant bibliographies by topics / Tyramide signal amplification

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Academic literature on the topic 'Tyramide signal amplification'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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Journal articles on the topic "Tyramide signal amplification"

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Antonov, Stanislav A., Ekaterina V. Novosadova, Andrey G. Kobylansky, Vyacheslav Z. Tarantul, and Igor A. Grivennikov. "A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining." Journal of Histochemistry & Cytochemistry 67, no. 10 (July 11, 2019): 771–82. http://dx.doi.org/10.1369/0022155419864113.

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The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is increasingly used for detection of various macromolecules and metabolites in biological samples. Here, we present a detailed analysis of the CuAAC reaction conditions in cells and tissue sections. Using the optimized CuAAC conditions, we have devised a highly sensitive immunostaining technique, based on the tyramide signal amplification/catalyzed reporter deposition (TSA/CARD) method with a novel alkyne tyramide substrate. The described method offers improved detection threshold compared to conventional immunofluorescent staining and produces significantly lower non-specific background than TSA/CARD with fluorescent tyramides.

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heusden, Jimmy Van, Paul de Jong, Frans Ramaekers, Hélène Bruwiere, Marcel Borgers, and Gerda Smets. "Fluorescein-labeled Tyramide Strongly Enhances the Detection of Low Bromodeoxyuridine Incorporation Levels." Journal of Histochemistry & Cytochemistry 45, no. 2 (February 1997): 315–19. http://dx.doi.org/10.1177/002215549704500216.

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Immunocytochemical detection of bromodeoxyuridine (BrdU) labeling can be hampered by low BrdU incorporation levels. We describe here an amplification method for weak BrdU immunosignals. The tyramide signal amplification method based on catalyzed reporter deposition (CARD) uses fluorescein-labeled tyramide as a substrate for horseradish peroxidase. The enzyme catalyzes the formation of highly reactive tyramide radicals with a very short half-life, resulting in the binding of fluorescein-conjugated tyramide only at the site of the enzymatic reaction. MCF-7 cells were grown in vitro in medium containing charcoal-stripped fetal bovine serum supplemented by growth factors. Under these culture conditions, the BrdU immunosignal was hard to detect but could be enhanced specifically by the tyramide signal amplification system, resulting in clear-cut differences between BrdU-negative and BrdU-positive cells. This enabled rapid and objective quantification of the BrdU labeling index without the risk of underestimating the number of cells in S-phase. Therefore, this amplification of BrdU immunosignals might also prove valuable for in vivo cancer prognosis, cell kinetics studies, and computer-assisted image analyses.

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Speel, Ernst J. M., Frans C. S. Ramaekers, and Anton H. N. Hopman. "Sensitive Multicolor Fluorescence In Situ Hybridization Using Catalyzed Reporter Deposition (CARD) Amplification." Journal of Histochemistry & Cytochemistry 45, no. 10 (October 1997): 1439–46. http://dx.doi.org/10.1177/002215549704501013.

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We describe the simultaneous localization of DNA sequences in cell and chromosome preparations by means of differently fluorochrome-labeled (AMCA, FITC, TRITC) tyramides using the catalyzed reporter deposition (CARD) procedure. For this purpose, repeated as well as single-copy DNA probes were labeled with biotin, digoxigenin, and FITC, hybridized, and visualized with three different cytochemical detection systems based on horseradish peroxidase conjugates. These were sequentially applied to interphase nuclei and metaphase chromosomes at low concentrations to prevent crossreaction and nonspecific background. In situ localized peroxidase activity was visualized by the deposition of fluorochrome-labeled tyramide molecules. To allow specific deposition of a second and a third tyramide conjugate for multiple-target fluorescence in situ hybridization (FISH), remaining peroxidase activity was always completely inactivated by a mild acid treatment before application of the next peroxidase conjugate. The CARD reactions were optimized for maximal signal-to-noise ratio and discrete localization by tuning reaction time, H2O2, and tyramide concentrations. For both repeated and single-copy DNA targets, high FISH signal intensities were obtained, providing improvement of sensitivity over conventional indirect detection systems. In addition, the fluorescence CARD detection system proved to be highly efficient and easy to implement in multiple-labeling studies, such as reported here for FISH.

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Volante, Marco, Carla Pecchioni, and Gianni Bussolati. "Post-incubation Heating Significantly Improves Tyramide Signal Amplification." Journal of Histochemistry & Cytochemistry 48, no. 11 (November 2000): 1583–85. http://dx.doi.org/10.1177/002215540004801115.

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Zhang, Shouping, Bin Hu, Xiaojing Xia, Yanzhao Xu, Bolin Hang, Jinqing Jiang, and Jianhe Hu. "Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification." Molecules 24, no. 23 (November 29, 2019): 4364. http://dx.doi.org/10.3390/molecules24234364.

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The frequent emergence of secondary infection and immunosuppression after porcine circovirus type 2 (PCV2) infection highlights the need to develop sensitive detection methods. A dual-signal amplification enzyme-linked immunosorbent assay (ELISA) based on a microplate coated with gold nanoparticle layers (GNPL) and tyramide signal amplification (TSA) was established. Results confirmed that the microplates coated with GNPL have a strong binding ability to the antibody without affecting the biological activity of the antibody. The microplates coated with GNPL have strong binding ability to the antibody, and the amplification of the tyramide signal is combined to further improve the sensitivity of PCV2. The PCV2 antibody does not crossreact with other viruses, demonstrating that the method has good specificity. A dual-signal amplification strategy is developed using microplates modified with GNPL and TSA to sensitively detect PCV2.

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Huang, Zhipeng, Qiuyuan Lin, Bin Yang, Xin Ye, Hui Chen, Wenhao Weng, and Jilie Kong. "Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization." Chemical Communications 56, no. 84 (2020): 12793–96. http://dx.doi.org/10.1039/d0cc04881j.

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Ness, Jayne M., Rizwan S. Akhtar, Cecelia B. Latham, and Kevin A. Roth. "Combined Tyramide Signal Amplification and Quantum Dots for Sensitive and Photostable Immunofluorescence Detection." Journal of Histochemistry & Cytochemistry 51, no. 8 (August 2003): 981–87. http://dx.doi.org/10.1177/002215540305100801.

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Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

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Wiedorn, Klaus Hermann, Torsten Goldmann, Christof Henne, Heike Kühl, and Ekkehard Vollmer. "EnVision+, a New Dextran Polymer-based Signal Enhancement Technique for In Situ Hybridization (ISH)." Journal of Histochemistry & Cytochemistry 49, no. 9 (September 2001): 1067–71. http://dx.doi.org/10.1177/002215540104900901.

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Seventy paraffin-embedded cervical biopsy specimens and condylomata were tested for the presence of human papillomavirus (HPV) by conventional in situ hybridization (ISH) and ISH with subsequent signal amplification. Signal amplification was performed either by a commercial biotinyl-tyramide-based detection system [GenPoint (GP)] or by the novel two-layer dextran polymer visualization system EnVision+ (EV), in which both EV–horseradish peroxidase (EV–HRP) and EV–alkaline phosphatase (EV–AP) were applied. We could demonstrate for the first time, that EV in combination with preceding ISH results in a considerable increase in signal intensity and sensitivity without loss of specificity compared to conventional ISH. Compared to GP, EV revealed a somewhat lower sensitivity, as measured by determination of the integrated optical density (IOD) of the positively stained cells. However, EV is easier to perform, requires a shorter assay time, and does not raise the background problems that may be encountered with biotinyl–tyramide-based amplification systems. (J Histochem Cytochem 49:1067–1071, 2001)

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Stanarius, Andreas, Heidi Faber-Zuschratter, Ingolf Töpel, Stefan Schulz, and Gerald Wolf. "Tyramide signal amplification in brain immunocytochemistry: adaptation to electron microscopy." Journal of Neuroscience Methods 88, no. 1 (April 1999): 55–61. http://dx.doi.org/10.1016/s0165-0270(99)00012-6.

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Wang, Guoji, Cristian L. Achim, Ronald L. Hamilton, Clayton A. Wiley, and Virawudh Soontornniyomkij. "Tyramide Signal Amplification Method in Multiple-Label Immunofluorescence Confocal Microscopy." Methods 18, no. 4 (August 1999): 459–64. http://dx.doi.org/10.1006/meth.1999.0813.

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Dissertations / Theses on the topic "Tyramide signal amplification"

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Chen, Wang-Wei, and 陳旺緯. "TSA-FISH (Tyramide Signal Amplification - Fluorescence in situ Hybridization) method applied in Fei-Tsui reservoir：Method development and assessment." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/39604233080878513278.

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"Highly Multiplexed Single Cell in situ Protein Analysis with Cleavable Fluorescent Probes." Doctoral diss., 2019. http://hdl.handle.net/2286/R.I.53605.

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abstract: Measurements of different molecular species from single cells have the potential to reveal cell-to-cell variations, which are precluded by population-based measurements. An increasing percentage of researches have been focused on proteins, for its central roles in biological processes. Immunofluorescence (IF) has been a well-established protein analysis platform. To gain comprehensive insights into cell biology and diagnostic pathology, a crucial direction would be to increase the multiplexity of current single cell protein analysis technologies. An azide-based chemical cleavable linker has been introduced to design and synthesis novel fluorescent probes. These probes allow cyclic immunofluorescence staining which leads to the feasibility of highly multiplexed single cell in situ protein profiling. These highly multiplexed imaging-based platforms have the potential to quantify more than 100 protein targets in cultured cells and more than 50 protein targets in single cells in tissues. This approach has been successfully applied in formalin-fixed paraffin-embedded (FFPE) brain tissues. Multiplexed protein expression level results reveal neuronal heterogeneity in the human hippocampus.
Dissertation/Thesis
Doctoral Dissertation Chemistry 2019

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Book chapters on the topic "Tyramide signal amplification"

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Faget, Lauren, and Thomas S. Hnasko. "Tyramide Signal Amplification for Immunofluorescent Enhancement." In Methods in Molecular Biology, 161–72. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2742-5_16.

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Ulloa-Navas, María José, Patricia García-Tárraga, Susana González-Granero, Pedro Pérez-Borreda, Vicente Herranz-Pérez, and José Manuel García-Verdugo. "Tyramide Signal Amplification for Immunoelectron Microscopy." In Neuromethods, 213–22. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1522-5_16.

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Wang, Herui, Ryan L. Pangilinan, and Yan Zhu. "Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence." In Methods in Molecular Biology, 89–97. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0247-8_7.

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Fominaya, Araceli, Yolanda Loarce, Juan M. González, and Esther Ferrer. "Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes." In Methods in Molecular Biology, 35–48. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3622-9_4.

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Lauter, Gilbert, Iris Söll, and Giselbert Hauptmann. "Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification." In Methods in Molecular Biology, 175–85. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-655-9_12.

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Lauter, Gilbert, Iris Söll, and Giselbert Hauptmann. "Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish." In Methods in Molecular Biology, 397–409. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9732-9_22.

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"Toward Molecular Sensitivity: Tyramide Signal Amplification in Molecular Morphology." In Molecular Morphology in Human Tissues, 129–44. CRC Press, 2004. http://dx.doi.org/10.1201/9780203503485-13.

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Hacker, Gerhard, Raymond Tubbs, James Pettay, Thomas Grogan, Annie Cheung, Richard Powell, and Cornelia Hauser-Kronberger. "Supersensitive In Situ Hybridization by Tyramide Signal Amplification and Nanogold® Silver Staining." In Gold and Silver Staining. CRC Press, 2002. http://dx.doi.org/10.1201/9781420040234.ch9.

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Conference papers on the topic "Tyramide signal amplification"

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Adler, Karl E., Mary C. Tyler, Alvydas Mikulskis, Mike O'Malley, Jeff J. Broadbent, Eva E. Golenko, Andy L. Johnson, Steve Lott, Anis H. Khimani, and Mark N. Bobrow. "Signal amplification on microarrays: techniques and advances in tyramide signal amplification (TSA)." In BiOS 2001 The International Symposium on Biomedical Optics, edited by Michael L. Bittner, Yidong Chen, Andreas N. Dorsel, and Edward R. Dougherty. SPIE, 2001. http://dx.doi.org/10.1117/12.427977.

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Roy, Sounak, Kenneth C. Valkenburg, and Kenneth J. Pienta. "Abstract 5192: Detection of disseminated prostate cancer cells in bone marrow using tyramide signal amplification." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-5192.

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Hurt, Stephen, Michael Campisano, and Stefan Letzsch. "Abstract 3880: Application of tyramide signal amplification technology to high content analysis of cellular kinase translocation assays." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3880.

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