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Journal articles on the topic 'UHPLC-ESI-MS'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 February 2022

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1

Sulc, Miloslav, Marie Eliasova, Zora Kotikova, and Jaromir Lachman. "Validation of a UHPLC-ESI-MS/MS method for anthocyanidin quantification in potato tubers." Czech Journal of Food Sciences 35, No. 3 (2017): 223–28. http://dx.doi.org/10.17221/389/2016-cjfs.

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The development of bioanalytical methods has become challenging due to sample complexity, requirements for method reliability, and speed of analysis with triple quadrupole LC-MS/MS used widely for the routine analysis of biological materials. The article presents the method development and validation results for pelargonidin and malvidin in potato tubers. The developed method uses a short C18 column, is able to measure all six common anthocyanidins, uses a binary mobile phase with acetonitrile and water both with added 1% formic acid, ESI ionisation in positive mode, 3-h hydrolysis with 2.7 M

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2

Wang, Baolin, Yimeng Lu, Rong Wang, Shumeng Liu, Xiaolong Hu, and Hao Wang. "Transport and metabolic profiling studies of amentoflavone in Caco-2 cells by UHPLC-ESI-MS/MS and UHPLC-ESI-Q-TOF-MS/MS." Journal of Pharmaceutical and Biomedical Analysis 189 (September 2020): 113441. http://dx.doi.org/10.1016/j.jpba.2020.113441.

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3

Wang, Jian, Wendy Cheung, and Willis Chow. "Ultra-High Performance Liquid Chromatography/Electrospray Ionization-Tandem Mass Spectrometry Determination of 151 Pesticides in Soybeans and Pulses." Journal of AOAC INTERNATIONAL 96, no. 5 (2013): 1114–33. http://dx.doi.org/10.5740/jaoacint.12-465.

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Abstract This paper presents the application of ultra-high performance LC (UHPLC) and MS for the determination of 151 pesticides in soybeans and pulses. A core-shell particle (2.6 μm particle size) column and a fully porous sub-2 μm (1.7 μm particle size) column showed comparable performance in chromatographic resolution and separation, increasing selectivity, and reducing analysis time. UHPLC was coupled with either a triple quadrupole mass analyzer (MS/MS) or a quadrupole Orbitrap (namely Orbital trap) mass spectrometer (Q-Orbitrap MS), which possesses fast data acquisition capability. Both

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4

Nannapaneni, Nagaraj Kumar, Sunil S. Jalalpure, Rajendraprasad Muppavarapu, and Sunil Kumar Sirigiri. "An ultra high performance liquid chromatography-tandem mass spectrometry method for the quantification of linagliptin in human plasma." RSC Advances 6, no. 71 (2016): 66756–66. http://dx.doi.org/10.1039/c6ra10450a.

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5

Wang, Jian, and Wendy Cheung. "UHPLC/ESI-MS/MS Determination of 187 Pesticides in Wine." Journal of AOAC INTERNATIONAL 99, no. 2 (2016): 539–57. http://dx.doi.org/10.5740/jaoacint.15-0265.

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Abstract This paper presents an ultra HPLC/electrospray ionization-tandem MS method to determine pesticides in wine. We adopted the quick, easy, cheap, effective, rugged, and safe (QuEChERs) method for extraction and used core-shell column to achieve ultra-HPLC to develop and validate a simple and fast method to analyze 187 pesticide residues in red and white wine samples. Pesticide residues were extracted from wine samples using QuEChERS. Ultra HPLC/electrospray ionization-tandem MS quantification was achieved using matrix-matched standard calibration curves with isotopically labeled standard

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6

Studzińska, Sylwia, Piotr Cywoniuk, and Krzysztof Sobczak. "Application of ion pair chromatography coupled with mass spectrometry to assess antisense oligonucleotides concentrations in living cells." Analyst 144, no. 2 (2019): 622–33. http://dx.doi.org/10.1039/c8an01603h.

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7

Liang, Jun, Xin-Dong Guo, and Fang Han. "UHPLC-ESI-MS/MS Quantitative Analyses of Multicomponent Hu Gan Tablets." Molecules 24, no. 23 (2019): 4241. http://dx.doi.org/10.3390/molecules24234241.

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Nowadays, the analysis of the multi-components in Chinese patent medicine prescriptions is being paid more attention. Therefore, in this study for the first time, a simple, rapid ultrahigh performance liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was established for simultaneous determination of 18 active compounds in a Chinese patent medicine of Hu Gan tablets (HGT) from different pharmaceutical factories in China. This task has met great emerging challenges from not only structural complexities and similarities but also co-occurrence of water

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8

Wang, Jian, Willis Chow, and Daniel Leung. "Applications of LC/ESI-MS/MS and UHPLC/Qq-TOF-MS for the Determination of 141 Pesticides in Tea." Journal of AOAC INTERNATIONAL 94, no. 6 (2011): 1685–714. http://dx.doi.org/10.5740/jaoacint.sgewang.

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Abstract This paper presents the applications of LC-electrospray ionization (ESI)/MS/MS and ultra-HPLC (UHPLC)/ESI quadrupole (Qq)-time-of-flight (TOF) MS for the determination of 141 pesticides in tea. Pesticides were extracted and cleaned up from tea with a modified quick, easy, cheap, effective, rugged, and safe method using graphitized carbon black and primary-secondary amine sorbents. Quantification was achieved using matrix-matched standard calibration curves with isotopically labeled standards or a chemical analog as internal standards in an analytical range from 5 to 500 μg/kg. The LC/

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9

Silva, Caio de Oliveira Gorgulho, Tallyta Santos Teixeira, Kelly Barreto Rodrigues, et al. "Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity." Analytical Methods 12, no. 2 (2020): 149–61. http://dx.doi.org/10.1039/c9ay01774g.

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Two new mass spectrometry methods, MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS, were developed for the characterization of cellulose-active lytic polysaccharide monooxygenases, expanding the analytical toolbox for the study of these enzymes.

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10

Ma, You-Ning, Wen-Jun Gui, and Guo-Nian Zhu. "The analysis of azocyclotin and cyhexatin residues in fruits using ultrahigh-performance liquid chromatography-tandem mass spectrometry." Analytical Methods 7, no. 5 (2015): 2108–13. http://dx.doi.org/10.1039/c4ay02624a.

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Ultrahigh-performance liquid chromatography coupled to electrospray positive ionization and tandem mass spectrometry (UHPLC-ESI(+)-MS/MS) was applied for the quantification and identification of azocyclotin and cyhexatin in fruit samples.

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11

Yang, Chun-Ju, Wu-Hsun Chung, and Wang-Hsien Ding. "Optimization of double-vortex-assisted matrix solid-phase dispersion for the rapid determination of paraben preservative residues in leafy vegetables." RSC Advances 10, no. 58 (2020): 35557–64. http://dx.doi.org/10.1039/d0ra05658h.

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12

Xie, Qingling, Bin Li, Feibing Huang, et al. "Simultaneous Determination of 12 Bioactive Constituents in Bupi Yiqi No. 1 Recipe by Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry." Journal of AOAC INTERNATIONAL 103, no. 1 (2020): 117–23. http://dx.doi.org/10.5740/jaoacint.19-0162.

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Abstract Background: Bupi Yiqi No. 1 Recipe (BPYQ), a traditional Chinese medicine formula, has been widely used as a treatment of multidrug resistance during chemotherapy in colorectal cancer and for the prevention of gastrointestinal cancer recurrence for decades. Objective: In this study, a rapid, reliable, and accurate ultrahigh-performance LC (UHPLC) coupled with electrospray ionization (ESI)–tandem MS (MS/MS) method was developed for the simultaneous determination of 12 major bioactive components, including protocatechuic acid, astilbin, rutin, calycosin-7-O-β-D-glucoside, ginsenoside Re

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13

Chen, Yung-Chih, Yi-Ting Ao, and Wang-Hsien Ding. "Determination of microcystins in water samples by deep eutectic solvent-based vortex-assisted liquid–liquid microextraction coupled with ultrahigh-performance liquid chromatography-high resolution mass spectrometry." RSC Advances 9, no. 66 (2019): 38669–76. http://dx.doi.org/10.1039/c9ra07544e.

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14

Sharma, Yashaswini, Ravikishore Velamuri, John Fagan, and Jim Schaefer. "Full-Spectrum Analysis of Bioactive Compounds in Rosemary (Rosmarinus officinalis L.) as Influenced by Different Extraction Methods." Molecules 25, no. 20 (2020): 4599. http://dx.doi.org/10.3390/molecules25204599.

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Rosmarinus officinalis is a potent antioxidant herb rich in polyphenols. Ultra-high-performance liquid chromatography, coupled with electrospray ionization and quadrupole-time of flight mass spectrometry (UHPLC-ESI-QTOF-MS), enables an exhaustive, full-spectrum analysis of the molecular constituents of natural products. The study aimed to develop a rapid UHPLC method to contribute new insights into the phytochemical composition of rosemary and to assess the performance of nine different procedures for extraction. These include fresh tissue homogenization, fresh and dry leaf decoction, and thei

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15

Sim, Hee-Jung, Ji Kim, Kang Lee, and Jongki Hong. "Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS." Molecules 18, no. 5 (2013): 5235–50. http://dx.doi.org/10.3390/molecules18055235.

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16

Ofosu, Fred Kwame, Fazle Elahi, Eric Banan-Mwine Daliri, et al. "UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains." Food Chemistry 337 (February 2021): 127788. http://dx.doi.org/10.1016/j.foodchem.2020.127788.

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17

Kieliszek, Marek, and Stanisław Błażejak. "Speciation Analysis of Selenium in Candida utilis Yeast Cells Using HPLC-ICP-MS and UHPLC-ESI-Orbitrap MS Techniques." Applied Sciences 8, no. 11 (2018): 2050. http://dx.doi.org/10.3390/app8112050.

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Selenium plays a key role in the proper metabolism of living organisms. The search for new selenium compounds opens up new possibilities for understanding selenometabolome in yeast cells. This study was aimed at the identification of compounds containing selenium in the feed yeasts Candida utilis ATCC 9950. Yeast biomass was kept in aqueous solutions enriched with inorganic selenium (20 mg·L−1) for 24 h. Speciation analysis of the element was performed using the HPLC-ICP-MS and UHPLC-ESI-Orbitrap MS techniques. The obtained selenium value in the yeast was 629 μg·g−1, while the selenomethionine

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18

Wu, Tao, Xiaoyu Wu, Xv Yuan, Yi Wang, Wenhua Zhou, and Weili Li. "Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis." Molecules 23, no. 9 (2018): 2138. http://dx.doi.org/10.3390/molecules23092138.

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The routine spermidine (SPD) detection method is time-consuming and laborious due to the lengthy chromatographic separation and/or tedious sample derivatization pretreatment. In this study, direct analysis in real-time ionization mode coupled with mass spectrometry (DART-MS) was developed to rapidly determine the SPD content of 12 bean cultivars. The results were compared in detail with those of the classical UHPLC-ESI-QTOF method. After conducting a series of optimizations, a simple sample extraction procedure employing 80% aqueous methanol, was followed by determination of sample extracts di

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19

Scollo, Emanuele, David Neville, M. Jose Oruna-Concha, Martine Trotin, and Rainer Cramer. "Characterization of the Proteome of Theobroma cacao Beans by Nano-UHPLC-ESI MS/MS." PROTEOMICS 18, no. 3-4 (2018): 1700339. http://dx.doi.org/10.1002/pmic.201700339.

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20

Bhagya, N., and K. R. Chandrashekar. "Identification and quantification of cytotoxic phenolic acids and flavonoids in Ixora brachiata by UHPLC-DAD and UHPLC-ESI-MS/MS." International Journal of Mass Spectrometry 450 (April 2020): 116290. http://dx.doi.org/10.1016/j.ijms.2020.116290.

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21

Wang, Mei, Ping Yu, Amar G. Chittiboyina, et al. "Characterization, Quantification and Quality Assessment of Avocado (Persea americana Mill.) Oils." Molecules 25, no. 6 (2020): 1453. http://dx.doi.org/10.3390/molecules25061453.

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Avocado oil is prized for its high nutritional value due to the substantial amounts of triglycerides (TGs) and unsaturated fatty acids (FAs) present. While avocado oil is traditionally extracted from mature fruit flesh, alternative sources such as avocado seed oil have recently increased in popularity. Unfortunately, sufficient evidence is not available to support the claimed health benefit and safe use of such oils. To address potential quality issues and identify possible adulteration, authenticated avocado oils extracted from the fruit peel, pulp and seed by supercritical fluid extraction (

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22

Groessl, Michael, Antonio Azzollini, Philippe J. Eugster, Benoit Plet, Jean-Luc Wolfender, and Richard Knochenmuss. "Comparison of UHPLC-ESI-MS and Hadamard Transform Atmospheric Pressure Ion Mobility-ESI-MS for Rapid Profiling of Isomeric Flavonoids." CHIMIA International Journal for Chemistry 68, no. 3 (2014): 135–39. http://dx.doi.org/10.2533/chimia.2014.135.

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23

Kasiotis, Konstantinos M., Eirini Baira, Electra Manea-Karga, Dimitra Nikolopoulou, Konstantinos Ganas, and Kyriaki Machera. "Investigating a human pesticide intoxication incident: The importance of robust analytical approaches." Open Chemistry 19, no. 1 (2021): 107–18. http://dx.doi.org/10.1515/chem-2021-0193.

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Abstract A human intoxication incident attributed to pesticide abuse was investigated using cutting-edge analytical methodologies. An LC-ESI-MS/MS method, based on a hybrid solid-phase extraction protocol (hybrid-SPE), was applied for the detection and quantification of several pesticides and metabolites in human biological fluids. Concomitantly, an UHPLC-HRMS method was applied to investigate potential metabolites, assisted by a complementary GC-MS method to elucidate the presence of plausible pesticides co-formulants. The LC-ESI-MS/MS method exhibited acceptable mean recoveries at the lower

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24

Salgado, Francisco, Laura Albornoz, Carmen Cortéz, et al. "Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS." Molecules 23, no. 1 (2017): 54. http://dx.doi.org/10.3390/molecules23010054.

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25

Maršálek, Petr, Helena Modrá, Veronika Doubková, and Vladimír Večerek. "Simultaneous determination of ten anticoagulant rodenticides in tissues by column-switching UHPLC-ESI-MS/MS." Analytical and Bioanalytical Chemistry 407, no. 25 (2015): 7849–54. http://dx.doi.org/10.1007/s00216-015-8954-1.

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26

Schlotterbeck, Jörg, Malgorzata Cebo, Agnes Kolb, and Michael Lämmerhofer. "Quantitative analysis of chemoresistance-inducing fatty acid in food supplements using UHPLC–ESI-MS/MS." Analytical and Bioanalytical Chemistry 411, no. 2 (2018): 479–91. http://dx.doi.org/10.1007/s00216-018-1468-x.

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27

Kim, Kang Uk, Kyeong Jin Yoon, Suhong Park, Jong Cheol Lee, Hyo Youl Moon, and Myeong Hee Moon. "Exercise-induced recovery of plasma lipids perturbed by ageing with nanoflow UHPLC-ESI-MS/MS." Analytical and Bioanalytical Chemistry 412, no. 28 (2020): 8003–14. http://dx.doi.org/10.1007/s00216-020-02933-w.

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28

Najm, Omaima Adil, Faizul Helmi Addnan, Nur Fariha Mohd-Manzor, et al. "Identification of Phytochemicals of Phoenix dactylifera L. Cv Ajwa with UHPLC-ESI-QTOF-MS/MS." International Journal of Fruit Science 21, no. 1 (2021): 848–67. http://dx.doi.org/10.1080/15538362.2021.1939227.

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29

Li, Xican, Xiaojian Ouyang, Rongxin Cai, and Dongfeng Chen. "3′,8″-Dimerization Enhances the Antioxidant Capacity of Flavonoids: Evidence from Acacetin and Isoginkgetin." Molecules 24, no. 11 (2019): 2039. http://dx.doi.org/10.3390/molecules24112039.

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To probe the effect of 3′,8″-dimerization on antioxidant flavonoids, acacetin and its 3′,8″-dimer isoginkgetin were comparatively analyzed using three antioxidant assays, namely, the ·O2− scavenging assay, the Cu2+ reducing assay, and the 2,2′-azino bis(3-ethylbenzothiazolin-6-sulfonic acid) radical scavenging assay. In these assays, acacetin had consistently higher IC50 values than isoginkgetin. Subsequently, the acacetin was incubated with 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxy radicals (4-methoxy-TEMPO) and then analyzed by ultra-performance liquid chromatography coupled with electro

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30

Kurek, Eliza, Magdalena Michalska-Kacymirow, Anna Konopka, Olga Kościuczuk, Anna Tomiak, and Ewa Bulska. "Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry." Molecules 25, no. 12 (2020): 2870. http://dx.doi.org/10.3390/molecules25122870.

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A fit for purpose analytical protocol was designed towards searching for low molecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS) was used to determine the total selenium content in entire sprouts as well as in selected extracts or chromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate t

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31

Chen, Haifang, Yun Yao, Yuan Zhan, et al. "Application of UHPLC/ESI-Q-TOF-MS/MS to Identify Constituents of Erding Granule and Anti-hyperuricemia Effect." Current Pharmaceutical Analysis 15, no. 5 (2019): 465–86. http://dx.doi.org/10.2174/1573412914666180612085117.

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Background: Erding granule (EDG) widely used as an agent with the effect of heat-clearing, detoxifying, eliminating dampness, relieving jaundice and upper respiratory tract disease in clinical application, but the systematic chemical information and anti-hyperuricemia effect of EDG was still unclear. Methods: An ultra-high performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) method was utilized to rapidly identify the chemical constituents of EDG. The anti-hyperuricemia effect of EDG was evaluated based on t

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32

Aras, Abdülmelik, Muzaffer Silinsin, Muhammed Nuri Bingol, and Ercan Bursal. "Identification of Bioactive Polyphenolic Compounds and Assessment of Antioxidant Activity of Origanum acutidens." International Letters of Natural Sciences 66 (November 2017): 1–8. http://dx.doi.org/10.18052/www.scipress.com/ilns.66.1.

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The main objective of the current work is to search the antioxidant activities of Origanum acutidens leaves by using DPPH, FRAP and CUPRAC methods severally. Three well known antioxidant compounds (BHA, BHT and ascorbic acid) were used as standards for comparing the extracts. Also, phenolic compounds of Origanum acutidens leaves were identified by UHPLC-ESI-MS/MS. The high concentrations of rosmarinic acid (11158.99 ppb) quinic acid (3200.84 ppb) and naringenin (1238.45 ppb) were detected quantitatively.

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33

Zhong, Rui-Feng, Guo-Bo Xu, Zheng Wang, et al. "Identification of anti-inflammatory constituents from Kalimeris indica with UHPLC-ESI-Q-TOF-MS/MS and GC–MS." Journal of Ethnopharmacology 165 (May 2015): 39–45. http://dx.doi.org/10.1016/j.jep.2015.02.034.

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34

Wang, Jian, Daniel Leung, and Willis Chow. "Applications of LC/ESI-MS/MS and UHPLC QqTOF MS for the Determination of 148 Pesticides in Berries†." Journal of Agricultural and Food Chemistry 58, no. 10 (2010): 5904–25. http://dx.doi.org/10.1021/jf902747t.

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35

Rechek, Habiba, Ammar Haouat, Kaouther Hamaidia, et al. "Chemical Composition and Antioxidant, Anti-Inflammatory, and Enzyme Inhibitory Activities of an Endemic Species from Southern Algeria: Warionia saharae." Molecules 26, no. 17 (2021): 5257. http://dx.doi.org/10.3390/molecules26175257.

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Warionia saharae Benth. & Coss. (Asteraceae) is an endemic species of North Africa naturally grown in the southwest of the Algerian Sahara. In the present study, this species’ hydromethanolic leaf extract was investigated for its phenolic profile characterized by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS). Additionally, the chemical composition of W. saharae was analyzed by gas chromatography–mass spectrometry, and its antioxidant potential was assessed through five in vitro tests: DPPH● scavengi

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36

Zong, Shi-Yu, Han Han, Bing Wang, et al. "Fast Simultaneous Determination of 13 Nucleosides and Nucleobases in Cordyceps sinensis by UHPLC–ESI–MS/MS." Molecules 20, no. 12 (2015): 21816–25. http://dx.doi.org/10.3390/molecules201219807.

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37

Di Stefano, Vita, Rosa Pitonzo, Vita Giaccone та ін. "Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method". Natural Product Research 31, № 4 (2016): 482–86. http://dx.doi.org/10.1080/14786419.2016.1190718.

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38

Zhao, Can, Ling-Yu Han, Wei Ren, et al. "Metabolic profiling of tenacigenin B, tenacissoside H and tenacissoside I using UHPLC-ESI-Orbitrap MS/MS." Biomedical Chromatography 30, no. 11 (2016): 1757–65. http://dx.doi.org/10.1002/bmc.3750.

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39

Han, Lifeng, Mavis Boakye-Yiadom, Erwei Liu, et al. "Structural Characterisation and Identification of Phenylethanoid Glycosides fromCistanches deserticolaY.C. Ma by UHPLC/ESI-QTOF-MS/MS." Phytochemical Analysis 23, no. 6 (2012): 668–76. http://dx.doi.org/10.1002/pca.2371.

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40

Gao, Xin, Wenru Liu, Jun Mei, and Jing Xie. "Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS." Molecules 24, no. 24 (2019): 4609. http://dx.doi.org/10.3390/molecules24244609.

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Shewanella putrefaciens is a well-known specific spoilage organism (SSO) and cold-tolerant microorganism in refrigerated fresh marine fish. Cold-adapted mechanism includes increased fluidity of lipid membranes by the ability to finely adjust lipids composition. In the present study, the lipid profile of S. putrefaciens cultivated at 30, 20, 10, 4, and 0 °C was explored using ultra-high-pressure liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) to discuss the effect of lipid composition on cold-adapted tolerance. Lipidomic analysis detected a total of 27 l

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41

Mashayekhy Rad, Farshid, Javier Zurita, Philippe Gilles, et al. "Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy." Journal of The American Society for Mass Spectrometry 30, no. 1 (2018): 161–73. http://dx.doi.org/10.1007/s13361-018-2009-8.

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42

Zhang, Tianji, Xin Liu, Hongmei Li, et al. "Characterization of epimerization and composition of heparin and dalteparin using a UHPLC-ESI-MS/MS method." Carbohydrate Polymers 203 (January 2019): 87–94. http://dx.doi.org/10.1016/j.carbpol.2018.08.108.

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43

Buzgaia, Nawal, Soo Yee Lee, Yaya Rukayadi, Faridah Abas та Khozirah Shaari. "Antioxidant Activity, α-Glucosidase Inhibition and UHPLC–ESI–MS/MS Profile of Shmar (Arbutus pavarii Pamp)". Plants 10, № 8 (2021): 1659. http://dx.doi.org/10.3390/plants10081659.

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The genus Arbutus (Ericaceae) has been traditionally used in folk medicine due to its phytomedicinal properties, especially Arbutus pavarii Pamp. However, this plant has not been evaluated for its efficacy, quality, and consistency to support the traditional uses, potentially in treating diabetes. Despite previous studies that revealed the biological activities of A. pavarii as antioxidant and α-glucosidase inhibitory agents, scientific reports on the bioactive compounds that contribute to its health benefits are still scarce. Therefore, this research focused on the evaluation of antioxidant a

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44

Buzgaia, Nawal, Tahani Awin, Fakhri Elabbar, et al. "Antibacterial Activity of Arbutus pavarii Pamp against Methicillin-Resistant Staphylococcus aureus (MRSA) and UHPLC-MS/MS Profile of the Bioactive Fraction." Plants 9, no. 11 (2020): 1539. http://dx.doi.org/10.3390/plants9111539.

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Arbutus pavarii Pamp is a medicinal plant commonly used by local tribes in East Libya for the treatment of many diseases, such as gastritis, renal infections, cancer and kidney diseases. In this study, the antibacterial activity of the leaf and stem bark extracts of the plant against methicillin-resistant Staphylococcus aureus (MRSA), as well as the metabolite profiles of the bioactive fractions, was investigated. The antibacterial activity was determined by disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), while the microbial reduction

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Ben Haj Yahia, Imen, Yosr Zaouali, Maria Letizia Ciavatta, et al. "Polyphenolic Profiling, Quantitative Assessment and Biological Activities of Tunisian Native Mentha rotundifolia (L.) Huds." Molecules 24, no. 13 (2019): 2351. http://dx.doi.org/10.3390/molecules24132351.

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Phenolic profiling of ten plant samples of Mentha rotundifolia (L.) Huds. collected from different bioclimatic areas of Tunisia, was for the first time carried out by using a fast ultra-high-performance liquid chromatography (UHPLC)-high resolution tandem mass spectrometry (HRMS/MS) method on a Q Exactive platform equipped with an electrospray ionization (ESI) source. An intraspecific, interpopulation variability was evidenced and a total of 17 polyphenolic metabolites were identified and quantified by using the UHPLC-HRESIMS/MS method, here validated for specificity, linearity, limit of detec

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Galgano, Fernanda, Roberta Tolve, Teresa Scarpa, et al. "Extraction Kinetics of Total Polyphenols, Flavonoids, and Condensed Tannins of Lentil Seed Coat: Comparison of Solvent and Extraction Methods." Foods 10, no. 8 (2021): 1810. http://dx.doi.org/10.3390/foods10081810.

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The lentil seed coat is a waste by-product still rich in phenolic compounds, specifically condensed tannins. The effect of different solvents, as well as different processes, namely conventional solid–liquid extraction (CSLE) and ultrasound-assisted extraction (UAE), on the extraction yield of specific phenolic compound classes was studied. Four empirical two-parameter models were examined to select the one that better fit the experimental data obtained under different operating conditions. Additionally, ultra-high-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectr

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Im, Yeong Ran, Inhwan Kim, and Jihyun Lee. "Phenolic Composition and Antioxidant Activity of Purple Sweet Potato (Ipomoea batatas (L.) Lam.): Varietal Comparisons and Physical Distribution." Antioxidants 10, no. 3 (2021): 462. http://dx.doi.org/10.3390/antiox10030462.

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The outer layer of purple sweet potato is removed during processing; however, this layer serves as a potential source of phenolics, especially anthocyanins. Herein, the phenolic composition and antioxidant activity were determined for the inner and outer layers of five purple sweet potato cultivars (‘Sinjami’, ‘Jami’, ‘Danjami’, ‘Yeonjami’, and ‘Borami’) harvested in Korea. Anthocyanins were identified using ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometer (UHPLC-(ESI)-qTOF-MS) and ultra-high-performance liquid chroma

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Peixoto, Juliana, Gerardo Álvarez-Rivera, Rita C. Alves, et al. "Cherry stem infusions: antioxidant potential and phenolic profile by UHPLC-ESI-QTOF-MS." Food & Function 11, no. 4 (2020): 3471–82. http://dx.doi.org/10.1039/c9fo02693b.

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Poojary, Mahesha M., Brijesh K. Tiwari, and Marianne N. Lund. "Selective and sensitive UHPLC-ESI-Orbitrap MS method to quantify protein oxidation markers." Talanta 234 (November 2021): 122700. http://dx.doi.org/10.1016/j.talanta.2021.122700.

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Sankar, ASK, Shanmugasundaram Palani, and Ravichandiran Velayudham. "Quality by Design–Applied Liquid Chromatography-Tandem Mass Spectrometry Determination of Enzalutamide Anti-Prostate Cancer Therapy Drug in Spiked Plasma Samples." Analytical Chemistry Insights 12 (January 1, 2017): 117739011772677. http://dx.doi.org/10.1177/1177390117726776.

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This research article presents the Quality by Design (QbD)–finalised conditions for a method that uses liquid chromatography-tandem mass spectrometry for the determination of concentration of enzalutamide (ENZ), an atypical anticancer drug, in a drug formulation and in spiked plasma samples. Critical process attributes (CPA) considered to be the influential parameters in separation, identification, and quantification processes by ultrahigh-performance liquid chromatography-electrospray ionisation-tandem mass spectrometry (UHPLC-ESI-MS/MS) were organic content, buffer strength, pH modifier, flo

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