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Journal articles on the topic 'UHPLC-FD'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Boutin, Michel, Pamela Lavoie, Margot Beaudon, et al. "Mass Spectrometry Analysis of Globotriaosylsphingosine and Its Analogues in Dried Blood Spots." International Journal of Molecular Sciences 24, no. 4 (2023): 3223. http://dx.doi.org/10.3390/ijms24043223.

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Fabry disease (FD) is an X-linked lysosomal storage disorder where impaired α-galactosidase A enzyme activity leads to the intracellular accumulation of undegraded glycosphingolipids, including globotriaosylsphingosine (lyso-Gb3) and related analogues. Lyso-Gb3 and related analogues are useful biomarkers for screening and should be routinely monitored for longitudinal patient evaluation. In recent years, a growing interest has emerged in the analysis of FD biomarkers in dried blood spots (DBSs), considering the several advantages compared to venipuncture as a technique for collecting whole-blo

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2

Zolkeflee, Nur Khaleeda Zulaikha, Nurul Shazini Ramli, Azrina Azlan, and Faridah Abas. "In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS/MS Profile of Muntingia calabura Leaves Extract." Molecules 27, no. 1 (2022): 287. http://dx.doi.org/10.3390/molecules27010287.

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Anti-diabetic compounds from natural sources are now being preferred to prevent or treat diabetes due to adverse effects of synthetic drugs. The decoction of Muntingia calabura leaves was traditionally consumed for diabetes treatment. However, there has not been any published data currently available on the processing effects on this plant’s biological activity and phytochemical profile. Therefore, this study aims to evaluate the effect of three drying methods (freeze-drying (FD), air-drying (AD), and oven-drying (OD)) and ethanol:water ratios (0, 50, and 100%) on in vitro anti-diabetic activi

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3

Tomšíková, H., P. Solich, and L. Nováková. "Sample preparation and UHPLC-FD analysis of pteridines in human urine." Journal of Pharmaceutical and Biomedical Analysis 95 (July 2014): 265–72. http://dx.doi.org/10.1016/j.jpba.2014.03.012.

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4

Harp, Bhakti Petigara, Marianita Perez-Gonzalez, Hector M. Cordero-Nieves, Rachel S. Pandian, and Bryan K. Bowes. "Determination of Seven Manufacturing Impurities in FD&C Red No. 40 by Ultra-High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 103, no. 1 (2020): 235–43. http://dx.doi.org/10.5740/jaoacint.19-0179.

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Abstract Background: The U.S. Food and Drug Administration batch-certifies color additives to ensure that each lot meets published specifications for coloring food, drugs, and cosmetics. Objective: An ultra-high-performance LC (UHPLC) method was developed to determine seven manufacturing impurities in the monoazo color additive FD&C Red No. 40 (R40). The analytes consist of two intermediates, an impurity originating from one intermediate, a reaction by-product, and three subsidiary colors. The intermediates are 4-amino-5-methoxy-2-methylbenzenesulfonic acid [cresidine-p-sulfonic acid (CSA)

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5

Perrone, Alessandro, Susan Mohamed, Vincenzo Donadio, Rocco Liguori, and Manuela Contin. "A Rapid and Simple UHPLC-MS/MS Method for Quantification of Plasma Globotriaosylsphingosine (lyso-Gb3)." Molecules 26, no. 23 (2021): 7358. http://dx.doi.org/10.3390/molecules26237358.

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Fabry disease (FD) is a rare X-linked lysosomal storage disorder caused by α-galactosidase A gene (GLA) mutations, resulting in loss of activity of the lysosomal hydrolase, α-galactosidase A (α-Gal A). As a result, the main glycosphingolipid substrates, globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3), accumulate in plasma, urine, and tissues. Here, we propose a simple, fast, and sensitive method for plasma quantification of lyso-Gb3, the most promising secondary screening target for FD. Assisted protein precipitation with methanol using Phree cartridges was performed as sam

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6

Krzykowski, Andrzej, Stanisław Rudy, Renata Polak, et al. "Drying of Red Chili Pepper (Capsicum annuum L.): Process Kinetics, Color Changes, Carotenoid Content and Phenolic Profile." Molecules 29, no. 21 (2024): 5164. http://dx.doi.org/10.3390/molecules29215164.

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Studies were conducted focusing on the drying of chili pepper fruits (Capsicum annuum L.), cultivar Cyklon, using convective (AD), convective-microwave (AMD), vacuum (VD), and freeze-drying (FD) methods. The influence of the drying method and temperature on the kinetics of the process and selected quality attributes of the dried product were evaluated. It was demonstrated that the Midilli model best described the drying kinetics for all methods across the entire measurement range. FD and VD produced dried products with the highest brightness and the greatest value of the a* color parameter. Th

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7

Andrade, Mariana A., Pedro V. Rodrigues, Carolina Barros, et al. "Extending High Fatty Foods Shelf-Fife from Lipid Oxidation and Microbiological Contamination: An Approach Using Active Packaging with Pomegranate Extract." Coatings 13, no. 1 (2023): 93. http://dx.doi.org/10.3390/coatings13010093.

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The search for new forms to extend foodstuffs’ shelf-life through the use of natural compounds and extracts continues to grow among researchers and the industry. In this line of thought, the main objective of this work was to develop, characterize and evaluate the effectiveness of an active food packaging based on polylactic acid (PLA) and incorporated with natural extracts obtained from grape and/or pomegranate by-products. In vitro, antioxidant methods were applied to evaluate the antioxidant capacity of three extracts: wort extract, freeze-dried pomegranate peels extract (PPE-FD), and natur

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8

Palomino-Vasco, Mónica, María Isabel Acedo-Valenzuela, María Isabel Rodríguez-Cáceres, and Nielene Mora-Diez. "Monitoring winemaking process using tyrosine influence in the excitation-emission matrices of wine." Food Chemistry 344 (November 25, 2020): 128721. https://doi.org/10.1016/j.foodchem.2020.128721.

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Abstract: Wine samples collected during the winemaking process have been analyzed employing a previously optimized UHPLC-FD method, determining their biogenic amines and amino acids profile. The results obtained have been submitted to a statistical analysis from which it was extracted that the most influential analyte was tyrosine. Thanks to its fluorescence, a method for its determination by excitation-emission matrices has been proposed. The accuracy of the method has been checked by means of Elliptical Joint Confidence Region test. The winemaking process has been mo

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9

Quintanilla-Villanueva, Gabriela Elizabeth, Araceli Sánchez-Álvarez, Raisa Estefanía Núñez-Salas, Melissa Marlene Rodríguez-Delgado, Donato Luna-Moreno, and Juan Francisco Villarreal-Chiu. "Recent Advances in Monitoring Microbial Toxins in Food Samples by HPLC-Based Techniques: A Review." Analytica 5, no. 4 (2024): 512–37. http://dx.doi.org/10.3390/analytica5040035.

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This study examines the significant impact of bacterial, algal, and fungal toxins on foodborne illnesses, and stresses the importance of advanced detection techniques, such as high-performance liquid chromatography (HPLC)-based methodologies. It emphasizes the urgent need for further advancements in these techniques to ensure food safety, as they offer significant benefits, including low detection limits and the ability to be combined with other techniques to detect a wide range of toxins. In this regard, HPLC has emerged as a versatile and sensitive analytical technique for this purpose. Vari

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10

Durai Ananda Kumar T, Sai Charan, Venkateswarlu A, and Supriya Reddy K. "Evolution of liquid chromatography: Technologies and applications." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (2020): 3204–11. http://dx.doi.org/10.26452/ijrps.v11i3.2449.

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Liquid chromatographic offers efficient analyte separation employing high pressure pumps. The reversed phase high performance liquid chromatography (RP-HPLC) is widely utilized in the purity testing and quantitative determination of pharmaceuticals and neutraceuticals. The limitations of traditional liquid chromatography such as particle size, resolution and selectivity demanded for the developments and Waters Corporation developed ultraperformance liquid chromatography (UPLC). Ultrafast liquid chromatography (UFLC) is another milestone, which offers faster and efficient separation. Multidimen

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11

Santana-Viera, Sergio, Alejandro Canino-Byreing, María Esther Torres-Padrón, et al. "Fabric Phase Sorptive Extraction for the Determination of Anthracyclines in Sewage." Separations 9, no. 3 (2022): 69. http://dx.doi.org/10.3390/separations9030069.

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Anthracyclines are a group of antineoplastic compounds used to treat acute leukemia and other cancers, and they are excreted after consumption by patients. These chemicals are often found in sewage at very low concentration levels. For this reason, the development of sensitive analytical methodologies capable of determining them at low concentrations is of prime importance. A simple, fast and sensitive analytical method using fabric phase sorptive extraction (FPSE) followed by ultra-high performance liquid chromatography with fluorescence detection (UHPLC-FD) has been developed and validated f

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12

Ingallina, Cinzia, Anatoly P. Sobolev, Simone Circi, et al. "Cannabis sativa L. Inflorescences from Monoecious Cultivars Grown in Central Italy: An Untargeted Chemical Characterization from Early Flowering to Ripening." Molecules 25, no. 8 (2020): 1908. http://dx.doi.org/10.3390/molecules25081908.

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The chemical composition of the inflorescences from four Cannabis sativa L. monoecious cultivars (Ferimon, Uso-31, Felina 32 and Fedora 17), recently introduced in the Lazio Region, was monitored over the season from June to September giving indications on their sensorial, pharmaceutical/nutraceutical proprieties. Both untargeted (NMR) and targeted (GC/MS, UHPLC, HPLC-PDA/FD and spectrophotometry) analyses were carried out to identify and quantify compounds of different classes (sugars, organic acids, amino acids, cannabinoids, terpenoids, phenols, tannins, flavonoids and biogenic amines). All

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13

Palomino-Vasco, Mónica, María Isabel Acedo-Valenzuela, María Isabel Rodríguez-Cáceres, and Nielene María Mora-Diez. "Automated chromatographic method with fluorescent detection todetermine biogenic amines and amino acids. Application to craft beerbrewing process." Journal of Chromatography A 1601 (April 24, 2019): 155–63. https://doi.org/10.1016/j.chroma.2019.04.063.

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Abstract: The combined determination of biogenic amines and amino acids is a challenge for food scientists. In this research, a new methodology for the automatic on-line precolumn derivatization and determination of 8 biogenic amines and 9 precursor amino acids by Ultra-High Performance Liquid Chromatography with fluorescent detection has been developed. The method derivatized the analytes with o-phthaldialdehyde and achieved the separation of the 17 derivatives in less than 15 minutes, obtaining good quality parameters (limits of detection varied between 7.00 and 210

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14

Palomino-Vasco, Mónica, María Isabel Rodríguez-Cáceres, Nielene Mora-Diez, Rosario Pardo-Botello, and María Isabel Acedo-Valenzuela. "Biogenic amines profile in red wines regarding aging and storage conditions." Journal of Food Composition and Analysis 83, no. 103295 (2019): 1–9. https://doi.org/10.1016/j.jfca.2019.103295.

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Abstract: The determination and quantification of eight biogenic amines in red wines without sample clean-up has been carried out by an ultra-high performance chromatographic method with fluorescent detection based on the o-phthaldialdehyde derivatization reaction. In these conditions, several monovarietal ‘Tempranillo’ wines (young, oak and aged ones) have been analyzed and the total concentrations of biogenic amines ranged between 22.2 and 73.4 mg L-1, which is in concordance with other Spanish red wines. No significant differences of total biogenic amine

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15

Boutin, Michel, Bruno Maranda, and Paula J. Waters. "Analysis of Globotriaosylceramide (Gb₃) in Liquid Urine: A Straightforward Assay Using Tandem Mass Spectrometry." Current Protocols 4, no. 6 (2024). http://dx.doi.org/10.1002/cpz1.1087.

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AbstractFabry disease (FD) is a lysosomal storage disorder caused by variants in the GLA gene encoding α‐galactosidase A, an enzyme required for catabolism of globotriaosylceramide (Gb3). Accumulation of Gb3 in patients’ cells, tissues, and biological fluids causes clinical manifestations including ventricular hypertrophy, renal insufficiency, and strokes. This protocol describes a methodology to analyze urinary Gb3 and creatinine. Samples are diluted with an internal standard solution containing Gb3(C17:0) and creatinine‐D3, centrifuged, and directly analyzed by ultra‐high performance liquid

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16

Bandeira, Nelson, Lucila Ribeiro, Tiele Rizzetti, et al. "Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS." Journal of the Brazilian Chemical Society, 2016. http://dx.doi.org/10.21577/0103-5053.20160240.

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17

Sójka, Michał, Agnieszka Hejduk, Lidia Piekarska-Radzik, Sylwia Ścieszka, Katarzyna Grzelak-Błaszczyk, and Elżbieta Klewicka. "Antilisterial activity of tannin rich preparations isolated from raspberry (Rubus Idaeus L.) and strawberry (Fragaria X Ananassa Duch.) fruit." Scientific Reports 15, no. 1 (2025). https://doi.org/10.1038/s41598-025-94731-6.

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Abstract The tannin rich preparations isolated from red raspberry (Rubus idaeus L.) and strawberry (Fragaria x ananassa Duch.) fruits were evaluated for their polyphenol composition and antimicrobial activity against six strains of Listeria monocytogenes, sourced from the ATCC collection. The preparations were obtained using solvent extraction with a water-acetone solution, followed by purification using Amberlite XAD 1600 resin. The resulting products, RTRP (raspberry tannin rich preparation) and STRP (strawberry tannin rich preparation), were characterized by their content of ellagitannins,

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