Academic literature on the topic 'UHPLC-UV'
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Journal articles on the topic "UHPLC-UV"
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Hamdy, Dalia A., and Tarek S. Belal. "A Comparative Study of Newly Developed HPLC-DAD and UHPLC-UV Assays for the Determination of Posaconazole in Bulk Powder and Suspension Dosage Form." Journal of Analytical Methods in Chemistry 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/241035.
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Objective. To develop and compare HPLC-DAD and UHPLC-UV assays for the quantitation of posaconazole in bulk powder and suspension dosage form.Methods. Posaconazole linearity range was 5–50 μg/mL for both assays. For HPLC-DAD assay, samples were injected through Zorbax SB-C18 (4.6 × 250 mm, 5 μm) column. The gradient elution composed of the mobile phase acetonitrile: 15 mM potassium dihydrogen orthophosphate (30 : 70 to 80 : 20, linear over 7 minutes) pumped at 1.5 mL/min. For UHPLC-UV assay, samples were injected through Kinetex-C18 (2.1 × 50 mm, 1.3 μm) column. The mobile phase composed of acetonitrile: 15 mM potassium dihydrogen orthophosphate (45 : 55) pumped isocratically at 0.4 mL/min. Detection wavelength was 262 nm in both methods.Results. The run time was 11 and 3 minutes for HPLC-DAD and UHPLC-UV assays, respectively. Both assays were linear (r2>0.999) with CV% and % error of the mean <3%. Limits of detection and quantitation were 0.82 and 2.73 μg/mL for HPLC-DAD and 1.04 and 3.16 μg/mL for UHPLC-UV, respectively. The methods quantitated PSZ in suspension dosage form with no observable interferences.Conclusions. Both assays were proven sensitive and selective according to ICH guidelines. UHPLC-UV assay exhibited some economic and chromatographic separation superiority.
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Guba, Andrea, Orsolya Bába, József Tőzsér, Éva Csősz, and Gergő Kalló. "Fast and Sensitive Quantification of AccQ-Tag Derivatized Amino Acids and Biogenic Amines by UHPLC-UV Analysis from Complex Biological Samples." Metabolites 12, no. 3 (2022): 272. http://dx.doi.org/10.3390/metabo12030272.
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Metabolomic analysis of different body fluids bears high importance in medical sciences. Our aim was to develop and validate a fast UHPLC-UV method for the analysis of 33 amino acids and biogenic amines from complex biological samples. AccQ-Tag derivatization was conducted on target molecules and the derivatized targets were analyzed by UHPLC-UV. The detection of the analytes was carried out with UV analysis and by Selected Reaction Monitoring (SRM)-based targeted mass spectrometry. The method was validated according to the FDA guidelines. Serum and non-stimulated tear samples were collected from five healthy individuals and the samples were analyzed by the method. The method was successfully validated with appropriate accuracy and precision for all 33 biomolecules. A total of 29 analytes were detected in serum samples and 26 of them were quantified. In the tears, 30 amino acids and biogenic amines were identified and 20 of them were quantified. The developed and validated UHPLC-UV method enables the fast and precise analysis of amino acids and biogenic amines from complex biological samples.
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Aljuffali, Ibrahim, Fahad Almarri, A. F. M. Motiur Rahman, Fars Kaed Alanazi, Musaed Alkholief, and Mohsin Kazi. "Simultaneous Determination of Cholecalciferol and 25- Hydroxycholecalceferol in Lipid-based Self-nanoemulsifying formulations and Marketed Product Vi-de 3® by UHPLC-UV." Current Pharmaceutical Analysis 16, no. 1 (2019): 100–109. http://dx.doi.org/10.2174/1573412915666190612141228.
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Background: The purpose of the current study was to develop a selective, precise, fast economical and advanced reverse phase ultra-high-performance liquid chromatography (UHPLC UV) method and validate it for the simultaneous estimation of cholecalciferol and its analogue 25- hydroxycholecalciferol in lipid-based self-nano emulsifying formulation (SNEDDS). Methods: The chromatographic separation was simply performed on a Dionex® UHPLC systems (Ultimate 3000, Thermo scientific) by using HSS C18 (2.1x50 mm, 1.8 µm) analytical column. The elution was carried out isocratically with the mobile phase consisting of acetonitrile and methanol in the ratio of 50:50 %v/v with a flow rate of 0.4 ml/min, followed by the UV detection at 265 nm. The injection volume was 1µl and the column temperature was maintained at 45°C. FDA regulatory guidelines were used to develop and validate the method. Results: The current developed UHPLC-UV method was found to be rapid (run time 2 min), and selective with the high resolution of cholecalciferol and 25-hydroxycholecalciferol (RT=0.530 min & 1.360 min) from different lipid matrices. The method was highly sensitive (Limit of Detection and Lower Limit of Quantification were 0.13 ppm & 0.51ppm, and 0.15 ppm & 0.54 ppm, respectively). The linearity, accuracy and precision were determined as suitable over the concentration range of 0.5-50.0 ppm for both the analytes. Conclusion: The proposed UHPLC-UV method can be used for the determination of cholecalciferol and 25-hydroxycholecalciferol in SNEDDS and marketed Vi-De 3® as pure forms (intact) with no interference of excipients or drug-related substances.
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Chen Kuang Piao, Christelle, Nouredine Sadeg, and Mann Youcef. "New UHPLC-UV method for plasma vitamin C determination." Annales de biologie clinique 69, no. 6 (2011): 735–37. http://dx.doi.org/10.1684/abc.2011.0630.
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Sun, Huiyue, Weiying Lu, and Boyan Gao. "Non-targeted detection of butter adulteration using pointwise UHPLC-ELSD and UHPLC-UV fingerprints with chemometrics." Food Chemistry 356 (September 2021): 129604. http://dx.doi.org/10.1016/j.foodchem.2021.129604.
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El-Hawary, Enas A., Ahmed Zayed, Annegret Laub, Luzia V. Modolo, Ludger Wessjohann, and Mohamed A. Farag. "How Does LC/MS Compare to UV in Coffee Authentication and Determination of Antioxidant Effects? Brazilian and Middle Eastern Coffee as Case Studies." Antioxidants 11, no. 1 (2022): 131. http://dx.doi.org/10.3390/antiox11010131.
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Coffee is a popular beverage owing to its unique flavor and diverse health benefits. The current study aimed at investigating the antioxidant activity, in relation to the phytochemical composition, of authenticated Brazilian green and roasted Coffea arabica and C. robusta, along with 15 commercial specimens collected from the Middle East. Ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI–HRMS) and UV spectrometry were employed for profiling and fingerprinting, respectively. With the aid of global natural product social molecular networking (GNPS), a total of 88 peaks were annotated as belonging to different chemical classes, of which 11 metabolites are reported for the first time in coffee seeds. Moreover, chemometric tools showed comparable results between both platforms, with more advantages for UV in the annotation of roasting products, suggesting that UV can serve as a discriminative tool. Additionally, antioxidant assays coupled with the UHPLC-ESI–HRMS dataset using partial least-squares discriminant analysis (PLS-DA) demonstrated that caffeoylquinic acid and caffeine were potential antioxidant markers in unroasted coffee versus dicaffeoyl quinolactone and melanoidins in roasted coffee. The study presents a multiplex metabolomics approach to the quality control of coffee, one of the most consumed beverages.
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Che Zain, Mohamad Shazeli, Muhamad Faris Osman, Soo Yee Lee, and Khozirah Shaari. "UHPLC-UV/PDA Method Validation for Simultaneous Quantification of Luteolin and Apigenin Derivatives from Elaeis guineensis Leaf Extracts: An Application for Antioxidant Herbal Preparation." Molecules 26, no. 4 (2021): 1084. http://dx.doi.org/10.3390/molecules26041084.
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Luteolin and apigenin derivatives present in oil palm (Elaeis guineensis) leaves (OPL) are reported to possess excellent antioxidant properties relating to numerous health benefits. To meet the global demand for flavonoids, OPL, which is plentifully generated as an agricultural by-product from oil palm plantations, can be further exploited as a new source of natural antioxidant compounds. However, to produce a standardized herbal preparation, validation of the quantification method for these compounds is required. Therefore, in this investigation, we developed and validated an improved and rapid analytical method, ultra-high-performance liquid chromatography equipped with ultraviolet/photodiode array (UHPLC-UV/PDA) for the quantification of 12 luteolin and apigenin derivatives, particularly focusing on flavonoid isomeric pairs: orientin/isoorientin and vitexin/isovitexin, present in various OPL extracts. Several validation parameters were assessed, resulting in the UHPLC-UV/PDA technique offering good specificity, linearity, accuracy, precision, and robustness, where the values were within acceptable limits. Subsequently, the validated method was employed to quantify luteolin and apigenin derivatives from OPL subjected to different drying treatments and extraction with various solvent systems, giving total luteolin (TLC) and apigenin content (TAC) in the range of 2.04–56.30 and 1.84–160.38 µg/mg extract, respectively. Additionally, partial least square (PLS) analysis disclosed the combination of freeze dry-aqueous methanol yielded OPL extracts with high TLC and TAC, which are strongly correlated with antioxidant activity. Therefore, we provide the first validation report of the UHPLC-UV/PDA method for quantification of luteolin and apigenin derivatives present in various OPL extracts, suggesting that this approach could be employed in standardized herbal preparations by adopting orientin, isoorientin, vitexin, and isovitexin as chemical markers.
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Reis, Naialy Fernandes Araújo, Luiz Felipe Gomes Silva, Mateus Araújo Castro e. Souza, et al. "UHPLC for quality evaluation of genuine and illegal medicines containing sildenafil citrate and tadalafil." Journal of Chromatographic Science 59, no. 1 (2020): 30–39. http://dx.doi.org/10.1093/chromsci/bmaa073.
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Abstract One of the highest incidences of illegal drug products is related to phosphodiesterase-5 inhibitors, used in treatment of erectile dysfunction, including those containing sildenafil citrate and tadalafil. In this context, comprehensive evaluation of the quality of genuine and illegal medicines was performed. A simple and rapid ultra-high performance liquid chromatography (UHPLC-UV) method to quantify sildenafil and tadalafil in the presence of six degradation products was developed and validated. Sildenafil and tadalafil were submitted to forced degradation. The separation was carried out on a Kinetex C18 (50 × 2.1 mm; 1.7 μm) column with mobile phase composed of acetonitrile and aqueous triethylamine solution. The calibration curves were linear in the range of 14–126 μg mL−1 for sildenafil citrate and 4–36 μg mL−1 for tadalafil and the method proved to be selective, precise, accurate and robust. Sildenafil degraded in oxidative media, whereas tadalafil degraded in acidic, alkaline and oxidative environment. The chemical structures and the mechanisms for the formation of the main degradation products were proposed by UHPLC coupled to tandem mass spectrometry. The UHPLC-UV method was applied in the pharmaceutical analysis of genuine and seized medicines. Some of them did not meet quality standards, mainly due to contents below specifications and the large variation on contents between units within a batch.
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Franco, Pedro H. C., Paula R. Chellini, Marcone A. L. Oliveira, and Gerson A. Pianetti. "Simultaneous Determination of First-Line 4-FDC Antituberculosis Drugs by UHPLC–UV and HPLC–UV: A Comparative Study." Journal of AOAC INTERNATIONAL 100, no. 4 (2017): 1008–15. http://dx.doi.org/10.5740/jaoacint.16-0200.
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Abstract Tuberculosis is the second most deadly infectious disease, surpassed only by HIV/AIDS, and has resulted in over 1 billion deaths in the last 200 years. The World Health Organization estimates that in 2014, 9.6 million people were infected by this disease and 1.5 million had died. First-choice treatment consists of fixed-dose combination tablets containing rifampicin, isoniazid, pyrazinamide, and ethambutol hydrochloride (4-FDC). There are pharmacopeial protocols available to test 4-FDC, but they are prolonged, two-step methods. One single-step method in the literature performs the simultaneous determination by HPLC, but requires a long acquisition time. In this context, an ultra-HPLC (UHPLC) method was developed based on the HPLC method with the objective of reducing analysis time. A C18 column (1.9 µm particle size) was used with UV–diode-array detection at 238 and 282 nm. The method was found to be selective, linear, exact, precise, and robust. Samples from two batches were analyzed and the results compared with those obtained by the HPLC method, with no statistically significant differences observed (P &gt; 0.05). This UHPLC method reduced the analysis time from 17 to 4 min, with a more than 90% reduction in sample and reagent consumption and a financial economy of almost 50-fold.
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Verdu, Cindy, Julia Gatto, Ingrid Freuze, Pascal Richomme, François Laurens, and David Guilet. "Comparison of Two Methods, UHPLC-UV and UHPLC-MS/MS, for the Quantification of Polyphenols in Cider Apple Juices." Molecules 18, no. 9 (2013): 10213–27. http://dx.doi.org/10.3390/molecules180910213.
Full textDissertations / Theses on the topic "UHPLC-UV"
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Gabrielli, M. "CHEMICAL MARKERS FOR THE EVALUATION OF SENSORY AND ANTIOXIDANT PROPERTIES OF WINES." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/230019.
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Sotolon (3-Hydroxy-4,5-dimethyl-2(5)-furanone) is a chiral lactone responsible for the oxidized flavour in fortified wines and wine produced by oxidative ageing. The perception threshold of the racemic mixture in wine is 8 – 10 μg/L and the flavour is reported as curry, aged honey, aged sake and fenugreek. Though it is considered a typical flavour note in Madera, Porto and Sherry, it is an off-flavour in dry white wine where its oxidative note is detrimental for the fresh taste and odour. The sotolon formation pathways, during winemaking, are affected by chemical and physical factors such as: oxygen concentration, storage temperature and time, reducing sugar concentration and antioxidant compounds concentration (e.g. sulphur dioxide or (GSH) glutathione). Due to the number of chemical and physical factors affecting the Sotolon formation in wine this compound has been suggested as chemical marker of white wine shelf-life.
A fast, sensitive and easy to apply analytical method (UHPLC-UV) and it was applied to the evaluation of SO2-free Franciacorta DOCG wines in order to assess the effect of different disgorgement conditions (antioxidant additives, ageing time and temperature) on the sotolon formation. The sotolon concentration was measured in sparkling wine stored at 15°C and 25°C for 6 months added to three different antioxidant preparations (2 g/hL and 4 g/hL) potentially substituting the sulfur dioxide. Furthermore, we investigated the chemical and physical factors could affect the sotolon formation in synthetic wine. Model solution conitaing increasing concentration of pentoses, GSH, amino group, catechin, oxygen, ethanal, tartaric acid and iron are stored at two temperatures (70°C and 5°C) for five days in order to clarify the compositive factors affecting the sotolon synthesis in white wine. Finally, we compared the performances of analytical methods (HPLC-UV and UHPLC-MS) for sotolon quantification, which were previously developed. Separately, we developed a fast, sensitive and easy to apply analytical method (UHPLC-UV) for the biogenic amines (BAs) assessment in red wine treated with different malolactic fermentation condition (Spontaneous MLF; Inoculum and Co-inoculum techniques). Moreover was checked the trend of intra and extra-cellular glutathione and their effect on the aromatic matrix of South African Sauvignon blanc (Stellenbosch) must and wine during the alcholic fermentation and aging. The Must was treated with GSH and a GSH-enriched inactive dry yeast preparation (GSH-IDYs).
The proposed analytical methods (UHPLC-UV; HPLC-UV and UHPLC-MS) provide a sample preparation faster and easier-to-apply than those previously reported for the routine analyses of sotolon. The methods (HPLC-UV; UHPLC-MS/MS; UHPLC-UV) were proved suitable for the determination of sotolon concentrations in white wine and in model solution under its sensory perception threshold. Two analytical methods compared (HPLC-UV; UHPLC-MS/MS) were successfully used for the screening of 70 commercial South African wines’ sotolon levels. The samples of Franciacorta sparkling wines treated with SO2 show the best protection against wine oxidation whereas the other commercial antioxidants tested caused detrimental effects due to the sotolon production. The phenolic composition of commercial antioxidants has influenced the production of sotolon. The sotolon formation tests carried out showed which sotolon can be formed by several formation pathways indeed it was generated under both reducing and oxidative conditions. Sotolon formation is enhanced by simultaneous presence of Fe++ and O2, of amino groups and of phenols. Glutathione inhibited the formation of sotolon only when it was simultaneously added with amino groups and phenols in an oxidizing environment or when it was present in oxidative conditions. Under anoxic conditions the sotolon formation test has been demonstrated that there is a high dependence between sotolon and reducing sugar contents, whereas tartaric acid and acetaldehyde didn't affect the formation of sotolon. The formation of
sotolon in anoxic environment has not yet been clarified and further tests will be conduct to understand the role of tartaric acid, ethanol, ethanal and ribose on sotolon production in synthetic wine. The analytical method for the BAs quantification showed good linearity and repeatability and was able to quantify the ABs in red wine. The preliminary results concerning the different malolactic fermentation conditions, suggest that co-inoculum technique does not seem to prevent BAs formation in wine. The trial data on GSH (intra and extra-cellular) have yet to be processed and will be assessed in the future.
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Seidlová, Bára. "Ergothionein a mykothiol v biosyntéze linkosamidů." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-415858.
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Specialized microbial metabolites are described as low-molecular-weight bioactive compounds, which are dispensable for the growth, evolution, or reproduction of its producer. This group of substances includes the lincosamides, which are produced mainly by the bacteria of the Streptomyces genera. Apart from other precursors, two low-molecular-weight thiols, ergothioneine and mycothiol, are essential participants of the lincosamide biosynthesis. Mycothiol (MSH) serves in this pathway as a source of sulphur, on the other hand, ergothioneine (ESH) constitutes a conjugate with the aminosugar moiety of lincosamide structure. The conjugate is condensed with an activated amino acid, which is catalyzed by an unusual enzyme to form a core of the lincosamide molecule. The objective of this diploma thesis is to isolate the conjugate of ESH and aminooctose, which serves as a substrate of the LmbD biosynthetic protein. Another aim is to study the links between the thiol metabolism and the biosynthesis of three lincosamides, lincomycin, celesticetin, and intervencin, which are produced by different bacterial strains. Bacterial strains were cultivated under laboratory conditions and methods of liquid chromatography with UV and MS detection were used for the analysis. The parameters of the methods were developed...
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Cudlman, Lukáš. "Analýza a izolace intermediátů biosyntetické dráhy alkylprolinových derivátů." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-397569.
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(EN) This work aims at preparation, analysis and isolation of intermediates of biosynthetic pathways of 4-alkyl-L-proline derivatives for their structural elucidation. Compounds with incorporated 4-alkyl-L-proline derivatives include clinically used lincosamide antibiotic, lincomycin A, antitumor pyrrolobenzodiazepines and bacterial hormone hormaomycin. Detailed knowledge of biosynthetic pathways of these biological active substances can be used to prepare new, more efficient derivatives. The first part of this work focuses on yellow-coloured dicarboxylic intermediates 1 and 2 of the biosynthetic pathway of 4-propyl-L-proline - the precursor of lincomycin A. In the presence of the methylation agent, S-adenosyl-L-methionine, and LmbW C- -methyltransferase, 1 was partially converted into intermediate 2. Using ultra-high performance liquid chromatography, both intermediates were identified from absorption and mass spectrometry spectra. A semi-preparative chromatographic method for isolation of both intermediates was developed. Surprisingly, a significantly lower stability of 2 compared to intermediate 1 was observed in an in vitro enzymatic reaction mixture. The second part of the work focuses on 4-ethylidene-L-proline - the precursor of tomaymycin belonging to pyrrolobenzodiazepines. After...
Conference papers on the topic "UHPLC-UV"
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Torrente-López, A., J. Hermosilla-Fernández, J. Hernández-Jiménez, J. Cabeza, A. Salmerón-García, and N. Navas. "3PC-065 Impact of light stress on the isoform profile of nivolumab (Opdivo) in opened vials estimated by (RP)UHPLC-UV-(HESI/Orbitrap)-MS." In 25th Anniversary EAHP Congress, Hospital Pharmacy 5.0 – the future of patient care, 23–28 March 2021. British Medical Journal Publishing Group, 2021. http://dx.doi.org/10.1136/ejhpharm-2021-eahpconf.40.
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Blebea, Nicoleta Mirela, and Simona Negreș. "METHODS FOR QUANTIFICATION OF THE MAIN CANNABINOIDS IN CBD OIL." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/13.
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Cannabidiol (CBD) is an alkaloid present in Cannabis sativa, together with tetrahydrocannabinol (THC) and more than 120 other substances belonging to a group of compounds named cannabinoids. Due to the continuous increased usage of CBD oils, it became necessary to be developed efficient methods for the identification of its compounds and especially for the characterization of the cannabinoids from the commercial specimens. Cannabinoids may be detected by many and different analytical methods, including immunoassays (EMIT®, Elisa, fluorescent polarization, radioimmunotest), techniques of flat chromatography: classic thin layer chromatography (TLC), optimum performance laminar chromatography (OPLC) and multiple development automatization (AMD), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry (HPLC-MS). Ultraviolet signal (UV) is used for the quantification of major cannabinoids and the mass spectrometer is used for the quantification of minor cannabinoids. The purpose of this study was to compare the performances of TLC, Ultra High-Performance Liquid chromatography with Photodiode Array Detection (UHPLC with PDA) and LC-MS/ MS technique for the qualitative and quantitative determination of cannabinoids in 3 commercial oils with CBD. Having in view that CBD may be found in many forms of oils, on the legal market of the internet, we believe that the development of a method for the qualitative and quantitative determination may be an interesting subject for the pharmaceutical professional persons.