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1

Motarjeme, Amir. "Ultrasound-Enhanced Thrombolysis." Journal of Endovascular Therapy 14, no. 2 (2007): 251–56. http://dx.doi.org/10.1583/1545-1550(2007)14[251:ut]2.0.co;2.

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2

Francis, Charles W. "Ultrasound-Enhanced Thrombolysis." Echocardiography 18, no. 3 (2001): 239–46. http://dx.doi.org/10.1046/j.1540-8175.2001.00239.x.

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3

Tsivgoulis, Georgios, and Andrei Alexandrov. "Ultrasound-Enhanced Thrombolysis." Stroke 39, no. 5 (2008): 1404–5. http://dx.doi.org/10.1161/strokeaha.107.505594.

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4

Motarjeme, Amir. "Ultrasound-Enhanced Thrombolysis." Journal of Endovascular Therapy 14, no. 2 (2007): 251–56. http://dx.doi.org/10.1177/152660280701400218.

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5

Holland, Christy K., Saurabh Datta, Sampada S. Vaidya, Jason M. Meunier, George J. Shaw, and Constantin‐C Coussios. "Ultrasound‐enhanced thrombolysis." Journal of the Acoustical Society of America 120, no. 5 (2006): 3002–3. http://dx.doi.org/10.1121/1.4787001.

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6

Holland, Christy K., Azzdine Y. Ammi, Saurabh Datta, et al. "0041: Ultrasound Enhanced Thrombolysis." Ultrasound in Medicine & Biology 35, no. 8 (2009): S8. http://dx.doi.org/10.1016/j.ultrasmedbio.2009.06.030.

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7

de Saint Victor, Marie, Dario Carugo, Constantin Coussios, and Eleanor P. Stride. "Ultrasound-enhanced thrombolysis: Mechanistic observations." Journal of the Acoustical Society of America 138, no. 3 (2015): 1820. http://dx.doi.org/10.1121/1.4933778.

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8

Alexandrov, Andrei V. "Ultrasound Enhanced Thrombolysis for Stroke." International Journal of Stroke 1, no. 1 (2006): 26–29. http://dx.doi.org/10.1111/j.1747-4949.2005.00012.x.

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9

Alexandrov, Andrei V. "Ultrasound Enhanced Thrombolysis for Stroke." Seminars in Cerebrovascular Diseases and Stroke 5, no. 2 (2005): 106–10. http://dx.doi.org/10.1053/j.scds.2005.12.004.

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10

Alexandrov, Andrei V. "Ultrasound enhanced thrombolysis: Clinical evidence." Journal of the Acoustical Society of America 117, no. 4 (2005): 2412. http://dx.doi.org/10.1121/1.4786282.

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11

Cui, Huizhong, and Xinmai Yang. "Laser-induced-cavitation enhanced ultrasound thrombolysis." Journal of the Acoustical Society of America 132, no. 3 (2012): 2038. http://dx.doi.org/10.1121/1.4755494.

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12

UCHINO, Ken, Mark A. MOEHRING, and Andrei V. ALEXANDROV. "Ultrasound Enhanced Thrombolysis for Ischemic Stroke." Neurosonology 16, no. 3 (2003): 139–45. http://dx.doi.org/10.2301/neurosonology.16.139.

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13

Li, Peiyang, Wenchang Huang, Jie Xu, Weiwei Shao, and Yaoyao Cui. "Efficacy Estimation of Microbubble-Assisted Local Sonothrombolysis Using a Catheter with a Series of Miniature Transducers." Micromachines 12, no. 6 (2021): 612. http://dx.doi.org/10.3390/mi12060612.

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Intravascular ultrasound has good prospects for clinical applications in sonothrombolysis. The catheter-based side-looking intravascular ultrasound thrombolysis (e.g., Ekosonic catheters) used in clinical studies has a high frequency (2 MHz). The lower-frequency ultrasound requires a larger-diameter transducer. In our study, we designed and manufactured a small ultrasound-based prototype catheter that can emit a lower frequency ultrasound (1.1 MHz). In order to evaluate the safety and efficacy of local low-frequency ultrasound-enhanced thrombolysis, a microbubble (MB) was introduced to augment
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14

Ebben, Harm P., Johanna H. Nederhoed, Rutger J. Lely, Willem Wisselink, and Kakkhee Yeung. "Microbubbles and UltraSound-accelerated Thrombolysis (MUST) for peripheral arterial occlusions: protocol for a phase II single-arm trial." BMJ Open 7, no. 8 (2017): e014365. http://dx.doi.org/10.1136/bmjopen-2016-014365.

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IntroductionAcute peripheral arterial occlusions can be treated with intra-arterial catheter-directed thrombolysis as an alternative to surgical thromboembolectomy. Although less invasive, this treatment is time-consuming and carries a significant risk of haemorrhagic complications. Contrast-enhanced ultrasound using microbubbles could accelerate dissolution of thrombi by thrombolytic medications due to mechanical effects caused by oscillation; this could allow for lower dosages of thrombolytics and faster thrombolysis, thereby reducing the risk of haemorrhagic complications. In this study, th
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15

Alexandrov, Andrei V. "Ultrasound-enhanced thrombolysis for stroke: clinical significance." European Journal of Ultrasound 16, no. 1-2 (2002): 131–40. http://dx.doi.org/10.1016/s0929-8266(02)00040-x.

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16

Tsivgoulis, Georgios, William C. Culp, and Andrei V. Alexandrov. "Ultrasound enhanced thrombolysis in acute arterial ischemia." Ultrasonics 48, no. 4 (2008): 303–11. http://dx.doi.org/10.1016/j.ultras.2007.11.008.

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17

Tsivgoulis, Georgios, Jürgen Eggers, Marc Ribo, et al. "Safety and Efficacy of Ultrasound-Enhanced Thrombolysis." Stroke 41, no. 2 (2010): 280–87. http://dx.doi.org/10.1161/strokeaha.109.563304.

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18

Moneta, G. L. "Ultrasound-Enhanced Systemic Thrombolysis for Acute Ischemic Stroke." Yearbook of Vascular Surgery 2006 (January 2006): 327–28. http://dx.doi.org/10.1016/s0749-4041(08)70252-5.

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19

Shaw, George J., Jason M. Meunier, Shao-Ling Huang, Christopher J. Lindsell, David D. McPherson, and Christy K. Holland. "Ultrasound-enhanced thrombolysis with tPA-loaded echogenic liposomes." Thrombosis Research 124, no. 3 (2009): 306–10. http://dx.doi.org/10.1016/j.thromres.2009.01.008.

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20

Rabinstein, A. A. "Ultrasound-Enhanced Systemic Thrombolysis for Acute Ischemic Stroke." Yearbook of Neurology and Neurosurgery 2006 (January 2006): 35–37. http://dx.doi.org/10.1016/s0513-5117(08)70255-2.

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21

Alexandrov, A. V., C. A. Molina, and J. C. Grotta. "Ultrasound-enhanced systemic thrombolysis for acute ischemic stroke." Journal of Vascular Surgery 41, no. 3 (2005): 559. http://dx.doi.org/10.1016/j.jvs.2004.12.013.

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22

Bader, Kenneth B., Matthew J. Gruber, and Christy K. Holland. "Shaken and Stirred: Mechanisms of Ultrasound-Enhanced Thrombolysis." Ultrasound in Medicine & Biology 41, no. 1 (2015): 187–96. http://dx.doi.org/10.1016/j.ultrasmedbio.2014.08.018.

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23

Alexandrov, Andrei V., Carlos A. Molina, James C. Grotta, et al. "Ultrasound-Enhanced Systemic Thrombolysis for Acute Ischemic Stroke." New England Journal of Medicine 351, no. 21 (2004): 2170–78. http://dx.doi.org/10.1056/nejmoa041175.

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24

Alexandrov, A. V., C. A. Molina, and J. C. Grotta. "Ultrasound-enhanced systemic thrombolysis for acute ischemic stroke." ACC Current Journal Review 14, no. 3 (2005): 23. http://dx.doi.org/10.1016/j.accreview.2005.02.031.

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25

Owens, Charles. "Ultrasound-Enhanced Thrombolysis: EKOS EndoWave Infusion Catheter System." Seminars in Interventional Radiology 25, no. 1 (2008): 037–41. http://dx.doi.org/10.1055/s-2008-1052304.

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26

Tsivgoulis, Georgios, and Andrei V. Alexandrov. "Ultrasound Enhanced Thrombolysis: Applications in Acute Cerebral Ischemia." Journal of Clinical Neurology 3, no. 1 (2007): 1. http://dx.doi.org/10.3988/jcn.2007.3.1.1.

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27

Shaw, G., N. Bavani, C. Lindsell, and C. K. Holland. "Ultrasound Contrast Agent Increases 120 kHz in-vitro Ultrasound Enhanced Thrombolysis." Academic Emergency Medicine 14, no. 5 Supplement 1 (2007): S35. http://dx.doi.org/10.1197/j.aem.2007.03.787.

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28

Meairs, Stephen, and Carl-Erik Dempfle. "In Vitro Models for Assessing Transcranial Ultrasound-Enhanced Thrombolysis." Stroke 36, no. 5 (2005): 929–31. http://dx.doi.org/10.1161/01.str.0000163112.92158.22.

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29

Cui, Huizhong, and Xinmai Yang. "Laser enhanced high-intensity focused ultrasound thrombolysis: Anin vitrostudy." Journal of the Acoustical Society of America 133, no. 2 (2013): EL123—EL128. http://dx.doi.org/10.1121/1.4778375.

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30

Datta, Saurabh, Azzdine Y. Ammi, Constantin C. Coussios, and Christy K. Holland. "Monitoring and simulating stable cavitation during ultrasound-enhanced thrombolysis." Journal of the Acoustical Society of America 122, no. 5 (2007): 3052. http://dx.doi.org/10.1121/1.2942879.

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31

Sekiguchi, Hirohito, Kenji Hamano, Sunao Yoshizawa, et al. "939-81 Thrombolysis Enhanced by Ultrasound on Intracoronary Thrombus." Journal of the American College of Cardiology 25, no. 2 (1995): 163A. http://dx.doi.org/10.1016/0735-1097(95)92132-o.

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32

Pfaffenberger, Stefan, Branka Devcic-Kuhar, Karem El-Rabadi, et al. "2MHz ultrasound enhances t-PA-mediated thrombolysis: comparison of continuous versus pulsed ultrasound and standing versus travelling acoustic waves." Thrombosis and Haemostasis 89, no. 03 (2003): 583–89. http://dx.doi.org/10.1055/s-0037-1613390.

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SummaryIn addition to fibrinolytic enzymes, ultrasound has the potential to enhance thrombolysis. High frequency ultrasound has the advantage that a combination of diagnostic and therapeutic ultrasound with only one device is possible. Therefore, we investigated the optimal high frequency (2 MHz) ultrasound field characteristics and application mode in vitro. Continuous ultrasound significantly enhanced rt-PA mediated thrombolysis: in a travelling wave field thrombolysis was augmented by 49.0 ± 14.7% and in a standing wave field by 34.8 ± 7.3%. In an intermittent application mode (1Hz, 10Hz, 1
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33

Xuedong Shen, Chandra Nair, Mark Holmberg, Aryan Mooss, Amy Arouni, and Dennis Esterbrooks. "Therapeutic Ultrasound-Enhanced Thrombolysis in Patients With Acute Myocardial Infarction." Angiology 61, no. 3 (2009): 253–58. http://dx.doi.org/10.1177/0003319709343287.

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34

Ammi, Azzdine Y., Yan Zhao, Aris Xie, Jonathan Lindner, Thomas R. Porter, and Sanjiv Kaul. "Ultrasound-enhanced thrombolysis in porcine clots in a flow system." Journal of the Acoustical Society of America 130, no. 4 (2011): 2502. http://dx.doi.org/10.1121/1.3654963.

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35

Datta, Saurabh, Constantin-C. Coussios, Azzdine Y. Ammi, T. Douglas Mast, Gabrielle M. de Courten-Myers, and Christy K. Holland. "Ultrasound-Enhanced Thrombolysis Using Definity® as a Cavitation Nucleation Agent." Ultrasound in Medicine & Biology 34, no. 9 (2008): 1421–33. http://dx.doi.org/10.1016/j.ultrasmedbio.2008.01.016.

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36

Shaw, George J., Jason M. Meunier, Christopher J. Lindsell, and Christy K. Holland. "Tissue Plasminogen Activator Concentration Dependence of 120 kHz Ultrasound-Enhanced Thrombolysis." Ultrasound in Medicine & Biology 34, no. 11 (2008): 1783–92. http://dx.doi.org/10.1016/j.ultrasmedbio.2008.03.020.

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37

Tsivgoulis, Georgios, and Andrei V. Alexandrov. "Ultrasound-enhanced thrombolysis in acute ischemic stroke: Potential, failures, and safety." Neurotherapeutics 4, no. 3 (2007): 420–27. http://dx.doi.org/10.1016/j.nurt.2007.05.012.

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38

Devcic-Kuhar, Branka, Stefan Pfaffenberger, Martin Grschl, Christian Kollmann, Ewald Benes, and Michael Gottsauner-Wolf. "In vitro thrombolysis enhanced by standing and travelling ultrasound wave fields." Ultrasound in Medicine & Biology 28, no. 9 (2002): 1181–87. http://dx.doi.org/10.1016/s0301-5629(02)00563-x.

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39

Larsson, J., J. Carlson, and S. B. Olsson. "Ultrasound enhanced thrombolysis in experimental retinal vein occlusion in the rabbit." British Journal of Ophthalmology 82, no. 12 (1998): 1438–40. http://dx.doi.org/10.1136/bjo.82.12.1438.

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40

Hitchcock, Kathryn E., Nikolas M. Ivancevich, Kevin J. Haworth, et al. "Ultrasound‐enhanced thrombolysis in an ex vivo porcine carotid artery model." Journal of the Acoustical Society of America 128, no. 4 (2010): 2440. http://dx.doi.org/10.1121/1.3508721.

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41

Ammi, Azzdine Y., Saurabh Datta, Stephen R. Perrin, et al. "120 kilohertz ultrasound‐enhanced thrombolysis in a porcine intracerebral hemorrhage model." Journal of the Acoustical Society of America 125, no. 4 (2009): 2713. http://dx.doi.org/10.1121/1.4784402.

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42

Hassanein, Mohamed, Yehia Saleh, Mandeep Randhawa, and Milind Karve. "Renal artery embolism successfully managed by ultrasound enhanced catheter directed thrombolysis." Egyptian Heart Journal 70, no. 4 (2018): 447–50. http://dx.doi.org/10.1016/j.ehj.2018.10.002.

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43

Engelberger, Rolf P., Verena Schroeder, Michael Nagler, et al. "Enhanced Thrombolysis by Ultrasound-Assisted Catheter-Directed Thrombolysis and Microbubbles in an In Vitro Model of Iliofemoral Deep Vein Thrombosis." Thrombosis and Haemostasis 119, no. 07 (2019): 1094–101. http://dx.doi.org/10.1055/s-0039-1688973.

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There is a need to improve the efficacy and safety of catheter-directed thrombolysis (CDT) for thrombo-occlusive diseases, and ultrasound-assisted CDT (USAT) is a promising approach. We tested if thrombolysis efficacy of USAT can be improved by adding gaseous microbubbles (MB). We developed an in vitro dynamic overflow model for iliofemoral deep vein thrombosis, and added MB to an USAT system with ultrasound energy and dose of tissue plasminogen activator according to clinical practice. A total of 64 clots (mean baseline weight of 8.23 ± 1.12 g, generated from citrated human whole blood from 7
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44

Lupi, Cristiana, Guido Lazzerini, Piero Chiarelli, Antonio L’Abbate, Daniele Rovai, and Giuseppina Basta. "Therapeutic effect of diagnostic ultrasound on enzymatic thrombolysis." Thrombosis and Haemostasis 91, no. 06 (2004): 1078–83. http://dx.doi.org/10.1160/th03-11-0684.

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SummaryIf delivered at elevated intensity, ultrasound potentiates enzymatic clot dissolution; however, an elevated acoustic intensity damages vascular wall and favors reocclusion. This study’s aim was to investigate whether exposure to high-frequency, lowintensity ultrasound generated by a diagnostic scanner enhances enzymatic thrombolysis, and if this effect differs in clots from blood of normal subjects and of patients with coronary artery disease (CAD). Venous blood samples were drawn from 10 healthy volunteers and from 10 CAD patients on chronic medical treatment, which also included aspir
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45

Meunier, Jason M., Christy K. Holland, Christopher J. Lindsell, and George J. Shaw. "Duty Cycle Dependence of Ultrasound Enhanced Thrombolysis in a Human Clot Model." Ultrasound in Medicine & Biology 33, no. 4 (2007): 576–83. http://dx.doi.org/10.1016/j.ultrasmedbio.2006.10.010.

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46

Chen, Xianghui, Francois Yu, Flordeliza Villanueva, et al. "LONG PULSED ULTRASOUND-ENHANCED THROMBOLYSIS: A NEW THERAPY FOR MYOCARDIAL NO-REFLOW." Journal of the American College of Cardiology 61, no. 10 (2013): E132. http://dx.doi.org/10.1016/s0735-1097(13)60133-3.

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47

Sharp, Andrew S. P. "Microbubble Enhanced Ultrasound-Assisted Catheter-Directed Thrombolysis for VTE—A Sound Idea?" Thrombosis and Haemostasis 119, no. 07 (2019): 1036. http://dx.doi.org/10.1055/s-0039-1692644.

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48

Korfhagen, Joseph J., Jason L. Raymond, Christy K. Holland, and George J. Shaw. "Effect of skull anatomy on intracranial acoustic fields for ultrasound-enhanced thrombolysis." Journal of the Acoustical Society of America 132, no. 3 (2012): 2065. http://dx.doi.org/10.1121/1.4755610.

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49

Nolte, Christian H., Florian Doepp, Stephan J. Schreiber, Lea M. Gerischer, and Heinrich J. Audebert. "Quantification of Target Population for Ultrasound Enhanced Thrombolysis in Acute Ischemic Stroke." Journal of Neuroimaging 23, no. 1 (2011): 79–81. http://dx.doi.org/10.1111/j.1552-6569.2011.00632.x.

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50

Balucani, Clotilde, and Andrei V. Alexandrov. "Ultrasound- and Microspheres-Enhanced Thrombolysis for Stroke Treatment: State of the Art." Current Cardiology Reports 12, no. 1 (2010): 34–41. http://dx.doi.org/10.1007/s11886-009-0082-0.

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