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Journal articles on the topic 'Ultrasound-Enzyme-Assisted extraction'

Author: Grafiati

Published: 31 August 2024

Last updated: 31 July 2025

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1

Singh, Gurpreet, Mohit Kumar, Ruchika Zalpouri, Pratik Pandit Potdar, Kamalpreet Singh, and Kulwinder Kaur. "Effects of different aqueous extraction techniques on physicochemical quality and oil recovery of sesame oil." Environment Conservation Journal 24, no. 1 (2023): 136–42. http://dx.doi.org/10.36953/ecj.11892309.

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Sesame is the oldest oilseed crop in agriculture, and it produces more oil than any other crop on the planet. This research aimed to investigate the impact of different oil extraction procedures on sesame seed oil physicochemical quality and oil recovery. The oil was extracted from the clean and healthy seeds using four extraction methods: aqueous, enzyme-assisted aqueous, ultrasound-assisted aqueous and solvent extraction using the Soxhlet apparatus. It was observed that ultrasound-assisted aqueous extracted oil had maximum saponification value and minimum acid value, refractive index, and lo

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Damar, İrem. "COMPARATIVE EVALUATION OF DIFFERENT EXTRACTION METHODS FOR ANTHOCYANIN RECOVERY FROM RED ONION PEEL: ENZYME-ASSISTED, ULTRASOUND-ASSISTED, AND ULTRASOUND-ASSISTED ENZYMATIC METHODS." Gıda 50, no. 3 (2025): 389–405. https://doi.org/10.15237/gida.gd25040.

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In this study, enzyme-assisted extraction (EAE), bath and probe type ultrasound-assisted extraction (UAE-B, UAE-P), and ultrasound-assisted enzymatic extraction using bath and probe-type ultrasound (UAEE-B, UAEE-P) were compared to conventional extraction (CE) for recovering anthocyanins from red onion peel. Extracts were analyzed for total phenolic (TPC), total flavonoid (TFC), total monomeric anthocyanin (TMA), antioxidant activities (AA-ABTS, AA-DPPH), individual anthocyanins, and colour parameters. UAEE-B achieved 33.12% higher extraction yield than CE. UAEE-B showed more TPC compared to o

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Divković, Laura, Aida Smajlagić, Emina Ahmetović, et al. "Comparative Analysis of Soxhlet and Ultrasound-assisted Extraction of Bioactive Components from Fig Leaves (lat. Ficus carica): Impact of the Method on Extraction Yield and Latex Preservation." International Research Journal of Pure and Applied Chemistry 26, no. 4 (2025): 14–26. https://doi.org/10.9734/irjpac/2025/v26i4924.

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This research investigates the efficiency of two methods for extracting bioactive compounds from fig leaves (Ficus carica) using different solvents. Dried fig leaves were used as extraction material. The focus is on Soxhlet extraction and Ultrasound-assisted extraction (UAE) methods. Also, two different solvents, methanol and ethanol, were used for extractions. Given that the enzyme ficin is one of the significant bioactive compounds in fig leaf latex, the aim was to explore and determine which method and solvent gives a higher extraction yield and better preservation of thermolabile component

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Li, Yang, Lian Zhou Jiang, Chen Wang, and Xiao Nan Sui. "The Comparison of Oil Quality from Different Processes." Applied Mechanics and Materials 66-68 (July 2011): 598–607. http://dx.doi.org/10.4028/www.scientific.net/amm.66-68.598.

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In this study, different oil extraction processes, such as extrusion pretreatment, ultrasound-ethanol assisted demulsification and traditional hexane extraction of oil, were employed to extract oil from soybean in order to compare their different effects on oil quality, fatty acid distribution and VE content of oil. The result shows that the quality of oil from extrusion pretreatment aqueous enzyme extraction (EAEP) of oil and ultrasound-ethanol assisted demulsification aqueous enzyme extraction of oil were same. The raw oil quality from the above two processes was better than the hexane extra

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Muhammad, Dimas Rahadian Aji, Siham Ayouaz, Annisa Noor Rachmawati, et al. "Advanced and Potential Methods for Extraction of Bioactive Compounds from Avocado Peel—A Review." Applied Sciences 14, no. 14 (2024): 6018. http://dx.doi.org/10.3390/app14146018.

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Extraction techniques are continuously developed by the scientific community. Meanwhile, avocado peel is a by-product of avocado processing and a source of bioactive compounds. The purpose of this review was to summarize the use of advanced techniques for extracting bioactive compounds from avocado peel to help understand which techniques have and have not been applied to avocado peel. Three primary databases were used to collect the information, including Google Scholar, Scopus, and Web of Science, by using the keywords “avocado”, “peel”, and “extraction”. Additional keywords related to the e

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Chen, Yang, Gonglin Cai, Hufu Chen, Huabin Zhou, Hang Qu, and Hailong Yang. "Physicochemical Properties and Biological Activities of Polysaccharides from Dendrobium officinale Leaves in Response to Different Extraction Methods." Foods 14, no. 12 (2025): 2029. https://doi.org/10.3390/foods14122029.

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Extraction techniques play a crucial role in determining the structural attributes and biological functions of polysaccharides. This study aimed to evaluate the physicochemical and biological properties of Dendrobium officinale leaf polysaccharides (DLPs) extracted using various methods, including hot water, acidic, alkali, ultrasound-assisted, and enzyme-assisted extraction. The results indicated that the extraction methods significantly influenced the yield, content, molar ratios of monosaccharides, molecular weight, and structural features of the polysaccharides, as well as their in vitro a

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Tamang, Bunu, Qi Liang, Biju Balakrishnan, Su Peng, and Wei Zhang. "Astragalus Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line." Nutrients 14, no. 11 (2022): 2333. http://dx.doi.org/10.3390/nu14112333.

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The chemical and nutritional constituents of mushrooms can alter significantly when grown on different substrates. Based on this fact, an approach was made to cultivate a new type of mushroom, Hengshan Astragalus Shiitake, by growing Shiitake mushrooms on beds supplemented with the roots of an edible herbal plant, Astragalus membranaceus. In this study, three green extraction techniques, including microwave-enzyme assisted (MEA), ultrasound-enzyme assisted (UEA) and microwave-ultrasound-enzyme assisted (MUEA) extractions, were used to compare both the yield and antiproliferative activity of th

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Usman, Muhammad, Mayuko Nakagawa, and Shuo Cheng. "Emerging Trends in Green Extraction Techniques for Bioactive Natural Products." Processes 11, no. 12 (2023): 3444. http://dx.doi.org/10.3390/pr11123444.

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This review explores eco-friendly methods for extracting bioactive natural products from diverse sources. The introductory exploration emphasizes the increasing demand for sustainable extraction methods, with a focus on the environmental impact of conventional approaches. Addressing existing knowledge gaps, this review outlines the key objectives of evaluating various green extraction technologies, including supercritical fluid extraction, pressurized liquid extraction, ultrasound-assisted extraction, enzyme-assisted extraction, and others. The primary findings underscore the remarkable potent

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Van Hung, Pham, Nguyen Hai Yen Nhi, Ling Yu Ting, and Nguyen Thi Lan Phi. "Chemical Composition and Biological Activities of Extracts from Pomelo Peel By-Products under Enzyme and Ultrasound-Assisted Extractions." Journal of Chemistry 2020 (February 26, 2020): 1–7. http://dx.doi.org/10.1155/2020/1043251.

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Enzyme-assisted extraction (EAE) and ultrasound-assisted extraction (UAE) were popular methods used to extract bioactive compounds from citrus peels, by-products of fruit processing industry. In this study, the total phenolic content (TPC), total flavonoid content (TFC), naringin and hesperidin contents, and antioxidant and antimicrobial activities of the extracts from pomelo peels using the combined enzyme and ultrasound-assisted extraction (E-UAE) or ultrasound and enzyme-assisted extraction (U-EAE) technique were investigated and compared with those extracted using the EAE and UAE. The opti

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10

Phan Van, Man, Duy Tran Duc, Hai Dam Thi Thanh, and Hai Tran Chi. "Comparison of ultrasound assisted extraction and enzyme assisted extraction of betacyanin from red dragon fruit peel." E3S Web of Conferences 187 (2020): 04004. http://dx.doi.org/10.1051/e3sconf/202018704004.

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This article was intended to extract betacyanin from the peel of red dragon fruit (Hylocereus polyrhizus) and used it as a natural colorant. In this study, enzyme and ultrasound techniques for the extraction of betacyanin from dried dragon fruit peel were compared. The ultrasonic power and sonication time levels were varied between 0.5, 1.5, 2.5, 3.5, and W/g; and 2.5, 5.0, 7.5, 10.0, and 12.5 min. The enzyme concentrations were 0.25, 0.75, 1.25, 1.75, 2.25, and 2.75 %v/w. The results revealed that the maximum betacyanin content obtained by the optimal UAE condition (3.5 W/g and 7.5 min) was 0

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Haque, Shaikh Manirul, Abuzar Kabir, Elaref Ratemi, et al. "Greener Pectin Extraction Techniques: Applications and Challenges." Separations 12, no. 3 (2025): 65. https://doi.org/10.3390/separations12030065.

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Pectin is a complex and versatile polysaccharide crucial for various industries. It functions as a thickener, gelling agent, emulsifier, and low-calorie food. Its anti-inflammatory and immunomodulatory properties have attracted biomedical interest, while its biodegradability and biocompatibility make it valuable for biomaterial applications. The effectiveness of these applications depends on the quality of pectin extraction procedures. While traditional extraction methods exist, green methodologies and alternative techniques have improved pectin’s physicochemical properties—a significant advan

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Sitorus, Randi, Tania Surya Utami, Yuswan Muharam, Rita Arbianti, and Naufal Azrizal Prasetyo. "Ekstraksi Daun Kejibeling (Strobilanthes crispus) dengan Metode Continuous Ultrasound-Assisted Enzymatic Extraction: Pengaruh Temperatur dan Konsentrasi Enzim." Jurnal Teknik Kimia USU 13, no. 2 (2024): 138–45. http://dx.doi.org/10.32734/jtk.v13i2.17151.

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Continuous ultrasound-assisted enzymatic extraction (CUAEE) method has become a promising technique for extracting bioactive compounds from plant materials. In this study, the extraction of kejibeling (Strobilanthes crispus) leaves was carried out using the CUAEE method to optimize the extract yield. The experimental design involved extraction parameters including leaf particle size (d ≤0,177 mm), ethanol concentration (50%), extraction time (180 minutes), and varying parameters such as temperature (30 °C, 40 °C, 50 °C, and 60 °C), as well as cellulase enzyme concentration of 3% and 5%. The re

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Mac, Hoa Xuan, and Triet Duy Anh Ngo. "ENZYME-ASSISTED AND ULTRASOUND-ASSISTED EXTRACTION OF SAPONIN FROM DANGSHEN (Codonopsis pilosula (Franch) Nannf) ROOTS." Scientific Journal of Tra Vinh University 1, no. 40 (2020): 83–91. http://dx.doi.org/10.35382/18594816.1.40.2020.619.

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This study was carried out to survey the effects of viscozyme L and ultrasound on the efficiency ofsaponin extraction with distilled water from Dangshen (Codonopsis pilosula (Franch) Nannf) roots. Ratio of materialto solvent, hydrolysis time, enzyme concentration, ultrasonic power were investigated. Results showed that the extraction efficiency was significantly increased when viscozyme L and ultrasound wereapplied. The highest content of saponin was 7,75 g/g of dry matter at the ratio of raw materials:to solvent of 1 : 15; hydrolysis time of 1,5 h; viscozyme L concentration of 0,15% (ml/g dry

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14

Výbohová, Veronika, and Katarína Hroboňová. "Isolation of Natural Compounds with Organoleptic Properties Using Selected Advanced Extraction Techniques." Chemické listy 117, no. 6 (2023): 365–72. http://dx.doi.org/10.54779/chl20230365.

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This review presents an overview of various advanced solid–liquid extraction techniques used for extraction of compounds with organoleptic properties from natural sources. These compounds are important additives to food, cosmetic, and pharmaceutical products. The most frequently used techniques are ultrasound assisted extraction and microwave assisted one, but there are other efficient techniques, such as pressurized liquid extraction, supercritical fluid extraction, pulsed electric field and enzyme assisted extraction. These techniques reduce or eliminate the volume of toxic solvents required

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15

Serea, Daniela, Gabriela Râpeanu, Oana Emilia Constantin, Gabriela Elena Bahrim, Nicoleta Stănciuc, and Constantin Croitoru. "Ultrasound and enzymatic assisted extractions of bioactive compounds found in red grape skins Băbească Neagră (Vitis vinifera) variety." Annals of the University Dunarea de Jos of Galati. Fascicle VI - Food Technology 45, no. 1 (2021): 9–25. http://dx.doi.org/10.35219/foodtechnology.2021.1.01.

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The phenolic composition of the skin of the Băbească neagră grapes grown in south-eastern Romania was studied by two extraction techniques, i. e., ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE). This study aimed to analyze the grape skin bioactive compounds and the antioxidant potential using ethanol of three different concentrations 50%, 70%, and 96% and acidified with acetic, citric acid, or hydrochloric acid. This research aimed to maximize the extraction of bioactive compounds from red grape skin by using commercially available oenological enzyme preparations, va

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16

Acoglu Celik, Busra, Muhammed Alpgiray Celik, Laura Jūrienė, et al. "Biorefining of Walnut Shells into Polyphenol-Rich Extracts Using Ultrasound-Assisted, Enzyme-Assisted, and Pressurized Liquid Extraction Coupled with Chemometrics." Foods 14, no. 13 (2025): 2245. https://doi.org/10.3390/foods14132245.

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Walnut (Juglans regia L.) shells are valuable agro-industrial by-products rich in polyphenols. This study investigated traditional (maceration) and advanced extraction techniques—ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), pressurized liquid extraction (PLE), and combined ultrasound–enzyme extraction (US-EAE)—to recover bioactive compounds from walnut shells. Extraction efficiency, total phenolic content (TPC), antioxidant capacity (ABTS•+, DPPH•), and polyphenol composition were evaluated. UPLC-ESI-MS/MS identified key polyphenols including ellagic acid, 4-hydroxyb

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17

Martins, Valter F. R., Tânia B. Ribeiro, Ana I. Lopes, Manuela E. Pintado, Rui M. S. C. Morais, and Alcina M. M. B. Morais. "Comparison among Different Green Extraction Methods of Polyphenolic Compounds from Exhausted Olive Oil Pomace and the Bioactivity of the Extracts." Molecules 29, no. 9 (2024): 1935. http://dx.doi.org/10.3390/molecules29091935.

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The use of by-products as a source of bioactive compounds with economic added value is one of the objectives of a circular economy. The olive oil industry is a source of olive pomace as a by-product. The olive pomace used in the present study was the exhausted olive pomace, which is the by-product generated from the air drying and subsequent hexane extraction of residual oil from the olive pomace. The objective was to extract bioactive compounds remaining in this by-product. Various types of green extraction were used in the present study: solvent extraction (water and hydroalcoholic); ultraso

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18

Puc-Santamaria, Cristhel Guadalupe, Rosa Us-Camas, Emanuel Hernández-Núñez, et al. "Ultrasonic-Assisted Enzymatic Extraction: An Innovative Technique for the Obtention of Betalains and Polyphenols from Dragon Fruit Peel." Processes 13, no. 7 (2025): 2307. https://doi.org/10.3390/pr13072307.

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Dragon fruit peel is a by-product rich in bioactive compounds, such as polyphenols and betalains. In this study, ultrasound-assisted enzyme extraction (UAEE) was proposed to exploit this, combining the advantages of the enzymatic hydrolysis and ultrasound extraction. The effect of extraction time, temperature, and enzyme quantity were evaluated using a Box–Behnken design. Total betalains and polyphenol contents were determined spectrophotometrically. The results show that the extraction of total polyphenols was significantly affected (p ≤ 0.05) by the enzyme quantity, while temperature had a s

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19

Hien, Tran Thi, and Nguyen Thi Minh. "Enhancing the extraction of pumpkin seed (Cucurbita pepo L) for increasing oil yield and its phytosterol content." Food Science and Applied Biotechnology 4, no. 1 (2021): 6. http://dx.doi.org/10.30721/fsab2021.v4.i1.104.

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Pumpkin seeds (Cucurbita pepo) were harvested in 2018 containing 41.23±1.23% oil; 33.18±1.61% protein; and 1575.97 ±67.79 mg phytosterol in 100g seeds (dried weight). Solvent (hexane) extraction (SE), Ultrasound assisted solvent (hexane) extraction (UAE), Enzyme Alcalase assisted extraction (EAE) and Enzyme Alcalase_Ultrasound assisted extraction (E_UAE) were studied. The oil’s yields and phytosterol in oil obtained were 90.07±0.17% and 1657.6±82.9 mg/100ml oil; 95.46±0.06% and 2017.5±100.1 mg/100ml oil; 89.65±0.11% and 1992.7±99.5 mg/100ml oil; 91.87± 0.03% and 2327.7 ±110.4 mg/100ml oil, res

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20

Vuong Hoai, Thanh, Phuc Nguyen Cao, My Phan Le Thao, et al. "Ultrasound-Assisted Enzymatic Extraction of Adenosine from Vietnamese Cordyceps militaris and Bioactivity Analysis of the Extract." Journal of Chemistry 2020 (May 26, 2020): 1–10. http://dx.doi.org/10.1155/2020/1487654.

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Vietnamese Cordyceps militaris (C. militaris) has long been recognized as one of the most valuable traditional Chinese medicines. In this study, adenosine was extracted from Vietnamese C. militaris by ultrasound-assisted enzymatic extraction method (UAEE) using water as a solvent. Then, the effects of five single factors on adenosine content including pH, enzyme-to-material ratio, ultrasonic power, ultrasonic time, and ultrasonic temperature were determined. After that, three factors consisting of ultrasonic power, ultrasonic time, and ultrasonic temperature were chosen based on their effects

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Zhou, Hengpu, Yingjie Dong, Zhangyun Wu, et al. "Ultrasound-assisted enzyme extraction of Dendrobium Officinale polysaccharides: Extraction process, characterization, immunomodulatory effects." Ultrasonics Sonochemistry 114 (March 2025): 107248. https://doi.org/10.1016/j.ultsonch.2025.107248.

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Thi Kim Loan, Le, Bui The Vịnh, and Ngo Van Tai. "ULTRASOUND-ENZYME ASSISTED EXTRACTION OF POLYPHENOL AND AMYLASE ENZYME FROM VIETNAMESE RAMIE LEAVES: PREDICTIVE MODELING DEVELOPMENT AND OPTIMIZATION STUDY." Journal of microbiology, biotechnology and food sciences 13, no. 5 (2024): e10700. http://dx.doi.org/10.55251/jmbfs.10700.

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Ramie leaves are rich in antioxidant polyphenols and amylase enzyme, which could be a good source for food applications. This study investigated the impact of combined ultrasound-enzyme treatment (U-EAT), including ultrasound intensity (10-30%), content of Viscozyme L. enzyme addition (0.5-1%) and extraction time (5-15 min) on polyphenol recovery, antioxidant activity, and amylase activity. The result revealed that U-EAT could enhance the efficiency of extraction, with a significantly higher yield of bioactive compounds and antioxidant capacity, and amylase enzyme activity compared to an untre

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23

Huynh, Dat T., Thien H. Nguyen, Ngan K. T. Nguyen, et al. "Optimization of ultrasound-assisted enzymatic extraction of betalains from red beetroot (Beta vulgaris L.)." Journal of Agriculture and Development 22, no. 06 (2023): 65–77. http://dx.doi.org/10.52997/jad.7.06.2023.

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Betalains in red beetroot (Beta vulgaris L.) offer health benefits and are commonly used as a food colorant. This study aimed to investigate betalains extraction using ultrasound-assisted enzymatic extraction (UAEE). The most significant factors involved in UAEE such as enzyme concentration, extraction temperature, and extraction time were studied and optimized using the response surface method (RSM) to achieve the highest betalains yield. The results showed that the optimal extraction conditions were as follows: enzyme concentration (32.1 U/mL), extraction temperature (40oC), and extraction t

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Šalić, Anita, Lina Šepić, Iva Turkalj, Bruno Zelić, and Dunja Šamec. "Comparative Analysis of Enzyme-, Ultrasound-, Mechanical-, and Chemical-Assisted Extraction of Biflavonoids from Ginkgo Leaves." Processes 12, no. 5 (2024): 982. http://dx.doi.org/10.3390/pr12050982.

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The biflavonoid extraction from ginkgo (Ginkgo biloba L.) leaves using solvent-based extraction with 70% ethanol, alone and in combination with enzyme-assisted, ultrasound-assisted, mechanical-assisted, and chemically assisted methods was investigated and the influence of extraction duration was explored. The total content of polyphenols, flavonoids, and phenolic acids in the extracts was determined spectrophotometrically, while individual biflavonoids were identified and quantified using HPLC-DAD. Amentoflavone, bilobetin, ginkgetin, isoginkgetin, and sciadopitysin were identified in all our

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Nguyen Tram Anh, Mai, Pham Van Hung, and Nguyen Thi Lan Phi. "Optimized Conditions for Flavonoid Extraction from Pomelo Peel Byproducts under Enzyme- and Ultrasound-Assisted Extraction Using Response Surface Methodology." Journal of Food Quality 2021 (January 15, 2021): 1–10. http://dx.doi.org/10.1155/2021/6666381.

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Pomelo (Citrus maxima (Burm.) Merrill) peel, which has been considered as a byproduct, is a potential source of flavonoids. In this study, the extraction conditions of total flavonoids from peels of Da Xanh pomelo were optimized using the response surface methodology with the Box–Behnken design. The combination of two novel extraction methods, including enzyme-assisted and ultrasound-assisted extractions, was used for the optimization. The results indicated that the optimal extraction condition was a liquid-to-solid ratio of 142.99 mL/g, enzyme concentration of 3.45%, and the time of 65.23 min

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Quitério, Eva, Clara Grosso, Ricardo Ferraz, Cristina Delerue-Matos, and Cristina Soares. "A Critical Comparison of the Advanced Extraction Techniques Applied to Obtain Health-Promoting Compounds from Seaweeds." Marine Drugs 20, no. 11 (2022): 677. http://dx.doi.org/10.3390/md20110677.

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Marine macroalgae are rich in bioactive compounds that can be applied in several fields, mainly food, cosmetics, and medicine. The health-promoting effects of bioactive compounds, such as polyphenols, polysaccharides, carotenoids, proteins, and fatty acids, have been increasingly explored, especially regarding their antioxidant activity and improvement in human health. To extract these valuable compounds, advanced technologies that include Supercritical-Fluid Extraction (SFE), Pressurised-Liquid Extraction (PLE), Ultrasound-Assisted Extraction (UAE), Microwave-Assisted Extraction (MAE), Enzyme

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Wang, Wenqian, Jinbi Li, Fuping Lu, and Fufeng Liu. "Ultrasound-Assisted Multi-Enzyme Extraction for Highly Efficient Extraction of Polysaccharides from Ulva lactuca." Foods 13, no. 6 (2024): 891. http://dx.doi.org/10.3390/foods13060891.

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Ulva polysaccharides present several physiological activities including antiviral, antitumor and anti-plasmodial effects. However, current processing usually results in low yields and high prices, thus lacking commercialization potential. The aim of this study was to develop an efficient method for the extraction of Ulva polysaccharides with high biological activity. The effect of cell wall-degrading enzymes including cellulase, hemicellulase, pectinase and protease on Ulva polysaccharide extraction was studied by statistical mixing design. Using the most effective enzyme preparations as the b

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Li, Qian, Zeyu Li, Chaogui Hu, Chenyue Wang, Feng Yang, and Xiaoqin Ding. "An Efficient Extraction, Characterization and Antioxidant Study of Polysaccharides from Peucedani Decursivi Radix." Plants 14, no. 14 (2025): 2188. https://doi.org/10.3390/plants14142188.

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Hot water extraction (HE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted extraction with NADES (UAE-NADES) and ultrasonic-assisted extraction with NADES and enzyme pretreatment (UAE-NADES-E) were employed to extract polysaccharides from Peucedani Decursivi Radix (PDR) and their structures were characterized for the first time. UAE-NADES-E was found to be the most effective extraction method, and the extraction process was optimized by Box–Behnken design (BBD)-response surface methodology (RSM) experiments. The optimal extraction process was determined by using a NADES system

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Miljanović, Anđela, Ana Bielen, Dorotea Grbin, et al. "Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Yield and Chemical Composition of Essential Oils." Molecules 25, no. 20 (2020): 4818. http://dx.doi.org/10.3390/molecules25204818.

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The effect of different hydrodistillation pretreatments, namely, reflux extraction, reflux extraction with the addition of cell wall-degrading enzymes, and ultrasound, on the yield and chemical composition of essential oils of sage, bay laurel, and rosemary was examined. All pretreatments improved essential oil yield compared to no-pretreatment control (40–64% yield increase), while the oil quality remained mostly unchanged (as shown by statistical analysis of GC-MS results). However, enzyme-assisted reflux extraction pretreatment did not significantly outperform reflux extraction (no-enzyme c

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Zhou, Liling, Jiaqian Luo, Qiutao Xie, Lvhong Huang, Dan Shen, and Gaoyang Li. "Dietary Fiber from Navel Orange Peel Prepared by Enzymatic and Ultrasound-Assisted Deep Eutectic Solvents: Physicochemical and Prebiotic Properties." Foods 12, no. 10 (2023): 2007. http://dx.doi.org/10.3390/foods12102007.

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Dietary fiber (DF) was extracted from navel orange peel residue by enzyme (E-DF) and ultrasound-assisted deep eutectic solvent (US-DES-DF), and its physicochemical and prebiotic properties were characterized. Based on Fourier-transform infrared spectroscopy, all DF samples exhibited typical polysaccharide absorption spectra, indicating that DES could separate lignin while leaving the chemical structure of DF unchanged, yielding significantly higher extraction yields (76.69 ± 1.68%) compared to enzymatic methods (67.27 ± 0.13%). Moreover, ultrasound-assisted DES extraction improved the properti

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Razak, Noorbina, M. Varghese Prince, Ann Annie Shaju, G. K. Rajesh, R. Sreeja, and Lilia Baby. "Ultrasound Assisted Hybrid Technologies for Extraction of Bioactive Compounds." Journal of Scientific Research and Reports 30, no. 12 (2024): 167–81. https://doi.org/10.9734/jsrr/2024/v30i122662.

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The sustainable and efficient extraction of bioactive compounds from natural resources offers a promising alternative to synthetic additives and formulations. These compounds including polyphenols, flavonoids and polysaccharides possess antioxidant, antimicrobial and many therapeutic effects rendering them highly demandable by various industries. Conventional extraction methods often exhibit drawbacks such as high energy consumption, excessive solvent usage, prolonged processing times, low extraction yields, and negative environmental impacts. To address these limitations, innovative extractio

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Peña-Portillo, Glenda-Caridad, Sergio-Miguel Acuña-Nelson, and José-Miguel Bastías-Montes. "From Waste to Wealth: Exploring the Bioactive Potential of Wine By-Products—A Review." Antioxidants 13, no. 8 (2024): 992. http://dx.doi.org/10.3390/antiox13080992.

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The present paper explores the biological potential of bioactive compounds present in wine industry wastes, highlighting their valorization to promote sustainability and circular economy. Wine by-products, such as grape pomace and vine shoots, contain a high concentration of polyphenols, flavonoids, anthocyanins and other phytochemicals with antioxidant, anti-inflammatory and anticarcinogenic properties. Both conventional extraction methods, such as solid–liquid extraction, and emerging technologies, including enzyme-assisted extraction, ultrasound-assisted extraction, supercritical fluid extr

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Makouie, Sina, Joanna Bryś, Jolanta Małajowicz, et al. "A Comprehensive Review of Silymarin Extraction and Liposomal Encapsulation Techniques for Potential Applications in Food." Applied Sciences 14, no. 18 (2024): 8477. http://dx.doi.org/10.3390/app14188477.

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This review explores advances in silymarin extraction and liposomal encapsulation techniques, highlighting their potential applications in the food, pharmaceutical, and cosmetic industries. The study evaluates a range of extraction techniques, including supercritical fluid extraction, microwave-assisted extraction, ultrasound-assisted extraction, and enzyme-assisted extraction, emphasising their efficiency and reduced environmental impact. Furthermore, it examines conventional and innovative liposomal encapsulation techniques, including supercritical carbon dioxide encapsulation and microfluid

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Angelina, Mauhibah Yumna, Abdullah, Rita Arbianti, Tania Surya Utami, and Heri Hermansyah. "The usage of enzyme in ultrasound-assisted enzymatic extraction method and its effect on yield extract from Keji Beling (Strobilanthes crispus.) leaves." E3S Web of Conferences 67 (2018): 03002. http://dx.doi.org/10.1051/e3sconf/20186703002.

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Green extraction process is a method which is used to obtain various plant extracts with minimum impact on the environment. Green extraction will reduce energy consumption, allow use of alternative solvents and renewable natural products, and ensure a safe and high quality extract. Ultrasound-assisted enzymatic extraction (UAEE) is one of green extraction method. UAEE is a mild, efficient and environmental friendly extraction method and it has been adopted for extracting various kinds of compounds. The use of enzyme will catalyze hydrolysis of the cytoderm and glycoproteins, therefore enhancin

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Tran, Chi Hai, Minh Tri Nghiem, Anh Minh Trinh Dinh, et al. "Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves." International Journal of Food Science 2023 (October 10, 2023): 1–12. http://dx.doi.org/10.1155/2023/7576179.

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Rubus alceifolius Poir (R.A. Poir) leaves are rich in phenolic compounds, offering many health benefits due to their incredible antioxidant potential. In this study, conditions for the ultrasound-assisted extraction (UAE) of phenolic compounds and antioxidant activity from R.A. Poir leaves were optimized using response surface methodology (RSM). This methodology assessed the effects of ultrasound power ( X 1 : 100-500 W), extraction temperature ( X 2 : 30-60°C), and extraction time ( X 3 : 5-55 min). The optimized UAE conditions were then compared with conventional extraction methods (Soxhlet

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Maskur, Muhammad, Mohammad Sayuti, I. Ketut Sumandiarsa, and Rahmatang . "Environmental-Friendly Extraction Methods to produce Bioactive Compounds in Seaweed." Research Journal of Chemistry and Environment 27, no. 11 (2023): 114–21. http://dx.doi.org/10.25303/2711rjce1140121.

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Seaweed is biological resource rich in bioactive compounds such as polysaccharides, polyphenols, proteins, pigments, fatty acids, polyamines, terpenoids and alkaloids that can be used in various food and non-food industries. The extraction method dramatically influences these bioactive compounds' amount and quality. In addition, environmental-friendly extraction methods are highly recommended, so this review provides an overview of the use of environmental-friendly, safe, affordable, effective and efficient extraction methods to produce maximum and sustainable bioactive compounds in seaweed. T

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Malvis Romero, Ana, José Julián Picado Morales, Leon Klose, and Andreas Liese. "Enzyme-Assisted Extraction of Ulvan from the Green Macroalgae Ulva fenestrata." Molecules 28, no. 19 (2023): 6781. http://dx.doi.org/10.3390/molecules28196781.

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Ulvan is a sulfated polysaccharide extracted from green macroalgae with unique structural and compositional properties. Due to its biocompatibility, biodegradability, and film-forming properties, as well as high stability, ulvan has shown promising potential as an ingredient of biopolymer films such as sustainable and readily biodegradable biomaterials that could replace petroleum-based plastics in diverse applications such as packaging. This work investigates the potential of Ulva fenestrata as a source of ulvan. Enzyme-assisted extraction with commercial cellulases (Viscozyme L and Cellulysi

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Nguyen, Ngoc-Van Thi, Chu Xuan Duong, Nha-Thao Ngoc Nguyen, et al. "Development of an extraction procedure to control rutin content from Azadirachta indica leaf extract for diabetes treatment." Journal of Multidisciplinary Sciences 6, no. 2 (2024): 17–24. https://doi.org/10.33888/jms.2024.623.

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Azadirachta indica, a widely planted plant in southern Vietnam, is used in traditional medicine for its anti-cancer, antibacterial, and diabetes-fighting properties. However, there are few studies on using rutin as a marker in A. indica. This study aimed to evaluate the alpha-glucosidase enzyme inhibitory activity of the extract for diabetes treatment. The extract was optimized using ultrasound-assisted extraction and quantified using HPLC/PDA. The HPLC/DAD method was developed to determine rutin in A. indica leaf using a Phenomenex C18 column. The extraction efficiency was evaluated by the pe

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Tanaka, Marcia Regina Rodrigues, Leonardo de Souza Mataruco, Elionio Galvão Frota, et al. "Lychee peel extract obtained by ultrasound-assisted extraction: bioactive compounds and functional properties." Acta Scientiarum. Technology 46, no. 1 (2024): e66447. http://dx.doi.org/10.4025/actascitechnol.v46i1.66447.

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The purpose of this study was to evaluate the impact of extracting solvent composition on the concentration of bioactive compounds and functional properties of lychee peel extracts obtained by ultrasound-assisted extraction. Five conditions were evaluated with different solvent proportions: 100% water, 25% ethanol and 75% water, 50% ethanol and 50% water, 75% ethanol and 25% water, and 100% ethanol. The use of an equitable mixture of water and ethanol (50% ethanol and 50% water) resulted in extracts with a higher content of total phenolic compounds (10964.3 mg EAG 100 g-1), antioxidant activit

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Xiong, Feng, Hui-Xian Liang, Zhi-Jing Zhang, et al. "Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods." Antioxidants 10, no. 11 (2021): 1842. http://dx.doi.org/10.3390/antiox10111842.

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Evodia lepta (E. lepta) is a traditional Chinese herbal medicine with various biological activities. One of the active components of this widely used medicinal plant is believed to be an oligosaccharide. The extraction yields, structural characteristics, antioxidant, and antitumor activities of four oligosaccharide extracts obtained by hot water extraction (HEO), ultrasound-assisted extraction (UEO), enzyme-assisted (EEO), and microwave-assisted extraction (MEO) were investigated. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS), X-ray diffraction (XR

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Jia, Chaoshuang, Fengmin Han, Xiaoyu Miao, Qiyue Zhang, Aonan Yan, and Peng Wu. "Study on optimization of extraction process of anthocyanin from cherry wine lees." Journal of Nutritional Health & Food Engineering 9, no. 1 (2019): 18–27. http://dx.doi.org/10.15406/jnhfe.2019.09.00321.

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This paper took cherry wine lees as the main raw material, and used three extraction methods (ultrasound-assisted enzymatic method, microwave extraction method, condensation reflux extraction method) to extract anthocyanin. The result showed that the extraction number of anthocyanins by ultrasonic-assisted enzymatic method was the highest. On the basis of single factor experiment, the response surface method was used for optimization. The impacts of experimental factors on extraction amount were successively the enzymolysis time, enzymolysis temperature, pH value, ultrasonic power, amount of e

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Phosarith, Nannapat, Thanyaporn Siriwoharn, and Wachira Jirarattanarangsri. "Comparison of the Conventional, Chemical, and Ultrasound Extraction of Crude Polysaccharides and Their Properties from Lentinula edodes (Berk.) Pegler." Foods 14, no. 14 (2025): 2428. https://doi.org/10.3390/foods14142428.

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This study aimed to compare the efficiency of four extraction methods, hot water (HW), hot alkaline (HA), ultrasound-assisted water (UW), and ultrasound-assisted alkaline (UA), for extracting crude β-glucan from Lentinula edodes, focusing on yield, functionality, and antidiabetic potential. The response surface methodology was used to optimize extraction conditions. Among all methods, UW yielded the highest β-glucan content (34.51 ± 0.82 g/100 g dry extract), indicating enhanced extraction efficiency through acoustic cavitation. However, HW demonstrated the most preserved structural integrity,

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Arbianti, R., H. Ningsih, T. S. Utami, Y. Muharam, and Slamet. "The effect of ultrasonic extraction methods on extract quality from Strobilanthes crispus L." IOP Conference Series: Earth and Environmental Science 963, no. 1 (2022): 012047. http://dx.doi.org/10.1088/1755-1315/963/1/012047.

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Abstract Strobilanthes crispus L. leaves are known to have many health benefits that have been proven both traditionally and in scientific research. To achieve more benefits from the leaves of Strobilanthes crispus L., the ultrasonic extraction (53 kHz) method was carried out using various ways. The variations of the ultrasonic method being carried out are UAE (Ultrasound-Assisted Extraction), UAEE (Ultrasound-Assisted Enzymatic Extraction), UAE-ATPE (Ultrasound-Assisted Enzymatic Aqueous Two-Phase Extraction) sequence and simultaneously. Extraction was carried out at 27 °C for 1hour, enzymati

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Fakhru-Nisa, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, and Syeda Taiba Bukhari. "Recent Approaches in the Extraction of Citrus Metabolites." Current Biotechnology 8, no. 2 (2020): 85–95. http://dx.doi.org/10.2174/2211550108666191210101516.

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The review summarizes the recent (2016-2019) practical applications of novel extraction methods for Citrus metabolite extraction, such as microwave-assisted (MAE), ultrasound-assisted (UAE), super-critical carbon dioxide (SC-CO2) and enzyme-assisted extraction (EAE) with the aim to compare efficiencies based on the criteria of enhanced (%) yield, cost-effectiveness, reproducibility, eco-friendliness, time/energy consumed and industrial scale application. It was found that supercritical carbon dioxide extraction (SC-CO2) is the most successful and scaled-up technique when compared to MAE, UAE,

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Kamal, AAA, M. Mohamad, KA Sulaiman, et al. "Optimization of Ultrasonic-Assisted Enzymatic Extraction of Tannic Acid from Chromolaena Odorota sp." Journal of Physics: Conference Series 2129, no. 1 (2021): 012081. http://dx.doi.org/10.1088/1742-6596/2129/1/012081.

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Abstract Previous study only implemented the time consuming and low amount of yield technique for extraction from Chromolaena odorata which is conventional method. Nonconventional extraction method with short extraction time and high amount of yield was applied in this study by applying ultrasound-assisted enzymatic extraction (UAEE). UAEE was used to extract tannic acid from Chromolaena odorata. The extraction parameters involved were enzyme concentration, sonication time and duty cycle at constant temperature of 50°C, solid to liquid ratio of 1:10 and sonication power at 60% amplitude. The o

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Gil-Martínez, Lidia, José Manuel de la Torre-Ramírez, Sofía Martínez-López, et al. "Green Extraction of Phenolic Compounds from Artichoke By-Products: Pilot-Scale Comparison of Ultrasound, Microwave, and Combined Methods with Pectinase Pre-Treatment." Antioxidants 14, no. 4 (2025): 423. https://doi.org/10.3390/antiox14040423.

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The revalorization of artichoke (Cynara scolymus L.) by-products is a promising strategy to obtain bioactive compounds with antioxidant properties, supporting a circular economy approach. This study compares the efficiency of an enzymatic pretreatment followed by microwave-assisted extraction (EMAE), ultrasound-assisted extraction (EUAE), and ultrasound-microwave-assisted extraction (EUMAE) at a pilot scale for recovering antioxidant compounds. Extracts were purified using Diaion® HP20 resin to obtain phenolic-rich fractions with enhanced antioxidant activity. The results showed that EUAE was

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Iftitah, Elvina Dhiaul, Warsito Warsito, Vivi Nurhadianty, Fitri Ariadna Sodi Miranda, and Rafika Nur Hafidah. "Extraction of <i>trans</i>-Anethole from Star Anise (<i>Illicium verum</i>) Using Combination of Microwave, Ultrasonic, and Enzyme Assisted Methods and Evaluation of Their Antibacterial Activity." Indonesian Journal of Chemistry 24, no. 2 (2024): 325. http://dx.doi.org/10.22146/ijc.79341.

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This study examines the efficiency of non-conventional extraction methods to obtain trans-anethole from Illicium verum using single and combination extraction techniques: microwave assisted extraction (MAE), ultrasound-assisted extraction (UAE), and enzyme assisted extraction (EAE) employing lipase from Aspergillus oryzae. All extraction methods were conducted using 96% ethanol (1:5 w/v) with varying time durations. The resulting product was an oleoresin, subsequently analyzed and separated using thin liquid chromatography (TLC) and column chromatography employing a solvent mixture of toluene

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Harun, Nur Fadilah, and Farah Hanim Ab Hamid. "An overview of the extraction methods of plant-based natural antioxidant compounds." Malaysian Journal of Chemical Engineering and Technology (MJCET) 4, no. 2 (2021): 73. http://dx.doi.org/10.24191/mjcet.v4i2.13055.

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Natural antioxidants are widely used in food additives, application in functional foods or also known as ingredients that offer health benefits that extend beyond their nutritional value, and pharmaceutical. These natural antioxidants such as carotenoids, vitamins, and polyphenol can be obtained from fruit, vegetables, and traditional herbal medicines. However, there have several issues regarding the conventional extraction method to extract natural antioxidants compounds from food and medicinal plants such as require a large volume of solvent and long extraction time. Therefore, new green ext

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Huamán-Castilla, Nils Leander, Luis Omar Mamani Apaza, Franz Zirena Vilca, Erick Saldaña, Yemina Karen Diaz-Valencia, and María Salomé Mariotti-Celis. "Comparative Analysis of Sustainable Extraction Methods and Green Solvents for Olive Leaf Extracts with Antioxidant and Antihyperglycemic Activities." Antioxidants 13, no. 12 (2024): 1523. https://doi.org/10.3390/antiox13121523.

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Olive leaves are agro-industrial waste that pose an environmental management problem. However, they contain polyphenolic compounds with important bioactive properties beneficial to human. This study aimed to evaluate the effectiveness of two extraction technologies (pressurized liquid extraction and ultrasound-assisted extraction) combined with green solvents (pure water, 15% ethanol, and 15% glycerol) at 50 °C and 70 °C. The goal was to obtain extracts with both antioxidant capacity and antihyperglycemic properties from olive leaves. Pressurized liquid extraction with 15% glycerol at 70 °C wa

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Pascariu, Oana-Elena, Luís Guimarães Dias, and Florentina Israel-Roming. "Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability." Horticulturae 10, no. 7 (2024): 743. http://dx.doi.org/10.3390/horticulturae10070743.

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Phenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) a

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