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Dissertations / Theses on the topic 'Unnatural amino acids'

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Published: 4 June 2021

Last updated: 25 July 2025

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1

Reeve, P. "Functionalising unnatural amino acids." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/19650/.

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2

Middleton, Richard John. "Synthesis of unnatural [alpha] - amino acids." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338866.

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3

Fryer, Andrew Mark. "Syntheses of natural and unnatural kainoid amino acids." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393580.

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4

Reddington, Samuel C. "Introducing novel protein functionality using unnatural amino acids." Thesis, Cardiff University, 2013. http://orca.cf.ac.uk/56226/.

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This thesis examines the tolerance and effects of unnatural amino acid (Uaa) incorporation into proteins in Escherichia coli using an expanded genetic code. Uaa incorporation was used to alter or install new properties in the target proteins, superfolder Green Fluorescent Protein (sfGFP) and cytochrome b562. Chapter 3 deals with the technical aspects of Uaa incorporation including orthogonality of the machinery and yield. Substitution of residues in sfGFP for unnatural analogues of tyrosine was shown to be a valuable way of altering the properties of the protein. Variants were generated with r

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5

Rodriguez, Erik Ali Tirrell David A. Dougherty Dennis A. "In Vivo Incorporation of Multiple Unnatural Amino Acids /." Diss., Pasadena, Calif. : California Institute of Technology, 2009. http://resolver.caltech.edu/CaltechETD:etd-01122009-153110.

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6

Bilgiçer, Zihni Basar. "Protein design using unnatural amino acids with fluorinated side chains /." Thesis, Connect to Dissertations & Theses @ Tufts University, 2005.

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Thesis (Ph.D.)--Tufts University, 2005.<br>Adviser: Krishna Kumar. Submitted to the Dept. of Chemistry. Includes bibliographical references. Access restricted to members of the Tufts University community. Also available via the World Wide Web;

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7

Valancogne, Ingrid. "Synthesis of unnatural amino acids and dipeptides for potential catalysts." Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300361.

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8

Wang, Jinfan. "In Vitro Kinetics of Ribosomal Incorporation of Unnatural Amino Acids." Doctoral thesis, Uppsala universitet, Struktur- och molekylärbiologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-282023.

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Ribosomal incorporation of unnatural amino acids (AAs) into peptides or proteins has found broad applications in studying translation mechanism, discovering potential therapeutics, and probing protein structure and function. However, such applications are generally limited by the low incorporation efficiencies of the unnatural AAs. With in vitro kinetics studies using a purified E. coli translation system, we found that the natural N-alkyl AA carrier, tRNAPro, could hasten the incorporation of N-methyl AAs. Also, the incorporation rate increased remarkably with increasing pH in the range of 7

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9

Bhushan, Bhaskar. "Unnatural amino acids as metal-mediated probes of biological function." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:4b1cbed6-1151-4b9f-ad97-aa5765cc9384.

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Conjugation reactions on proteins have been used to access various post-translational modifications, for targeted delivery of drugs, for microscopy, and in studying receptor-ligand interactions. However, the ability to modify native proteins is constrained by the reactive functionalities of naturally occurring amino acids. This has driven research into the incorporation of unnatural amino acids (UAAs) into proteins. Research in this area has been motivated both by the possibility of increasing the breadth of chemical techniques for protein modification by introducing novel 'bio-orthogonal' rea

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10

Drummond, Lorna J. "New methodology for the stereoselective synthesis of unnatural alpha-amino acids." Thesis, University of Glasgow, 2011. http://theses.gla.ac.uk/2827/.

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New general methodology for the stereoselective synthesis of unnatural alpha-amino acids has been developed. Early work focussed on investigating methods for the generation of chiral allylic alcohols using cross-metathesis of a simple enone with various terminal alkenes, followed by an asymmetric ketone reduction. The allylic alcohols were then converted to protected allylic amines via Overman rearrangement chemistry. Oxidative alkene cleavage and hydrolysis of these intermediates generated a range of alpha-amino acid targets. Attempts were also made to apply the developed methodology to the s

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11

Li, Shuwei Dervan Peter B. Roberts Richard W. "In vitro selection of mRNA-display libraries containing unnatural amino acids /." Diss., Pasadena, Calif. : California Institute of Technology, 2003. http://resolver.caltech.edu/CaltechETD:etd-05182003-185245.

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12

Halonski, John. "Utilization of Unnatural Amino Acids to Modulate Protein Structure and Function." W&M ScholarWorks, 2018. https://scholarworks.wm.edu/etd/1530192786.

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Proteins are capable of an astounding array of functions using only the 20 canonical amino acids; however, the ability to add new functional groups to the genetic code through the utilization of unnatural amino acids (UAAs) has greatly expanded our ability to study and manipulate proteins. By expanding the diversity of functional groups within proteins, a wide variety of applications in industry as well as in fields such as diagnostics, biochemistry, and materials science are now possible. These applications have further been expanded through the development and optimization of bioorthogonal r

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13

Gao, Lin. "The development of mRNA display : synthesis of peptides containing unnatural amino acids." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/8883.

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Currently, one of main challenges in drug discovery is the generation of diverse compound libraries that can be easily screened to identify potential inhibitors of therapeutic targets. mRNA display is a technique to create a vast library of unique peptide molecules that can be easily screened for this purpose. mRNA display generates peptides that are covalently linked to their encoding mRNA templates. The utilization of a reconstituted translation system makes it possible to incorporate unnatural amino acids with various structures into peptides. In this project, mRNA display and an E. col

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14

Klippenstein, Viktoria [Verfasser]. "Photoinactivation of glutamate receptors by genetically encoded unnatural amino acids / Viktoria Klippenstein." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2015. http://d-nb.info/1071088998/34.

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15

Mata, David Garcia. "Understanding Protein Structure And Function Using Rational Design And Unnatural Amino Acids." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338392020.

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16

Liu, Zhihua. "THE DESIGN AND SYNTHESIS OF NOVEL UNNATURAL AMINO ACIDS AND THE DESIGN AND SYNTHESIS OF PEPTIDES & PEPTIDOMIMETICS CONTAINING UNNATURAL AMINO ACIDS FOR THE STUDY OF G-PROTEIN COUPLED RECEPTORS." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/204274.

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Nature has gifted peptides as important modulators in the human body, but these types of molecules often have not been favored when we were looking for therapeutic agents. The poor bioavailability, fast degradation and until recent high manufacturing costs of some bioactive peptides lowered their potential usage in the health industry. Under these circumstances, unnatural amino acids were developed as indispensible tools providing enormous support to peptide science. By incorporating proper unnatural amino acids into a peptide or protein, we now can significantly improve peptide's or protein's

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17

Erickson, Sarah. "Using Unnatural Amino Acid Incorporation to Modify and Manipulate Adeno-Associated Virus:." Thesis, Boston College, 2020. http://hdl.handle.net/2345/bc-ir:108955.

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Thesis advisor: Eranthie Weerapana<br>Adeno-Associated Virus (AAV) has been developed into a powerful therapeutic tool - in the last ten years it has acted as a gene-delivery vehicle in several approved therapeutics and many more therapeutics on trial. Despite extensive research, gaps in our understanding of AAV’s infectious cycle still exist, and further development is needed for the creation of improved gene therapy vectors. Technology to incorporate Unnatural Amino Acids (UAAs) into the AAV capsid has recently been developed, and could aid in both furthering our understanding of AAV’s biolo

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18

Fowler, Lindsay S. "Synthesis of unnatural enone-containing α-amino acids : precursors to chiral N-heterocycles". Thesis, University of Glasgow, 2011. http://theses.gla.ac.uk/2524/.

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A fast and efficient synthetic route was developed for the synthesis of a novel class of enone-containing alpha-amino acid. An amino acid-derived beta-ketophosphonate ester was subjected to Horner-Wadsworth-Emmons conditions using a variety of aldehydes to produce a diverse library of alpha,beta-unsaturated amino acids. E-Configured enone-containing amino acids were also deprotected using a two-stage approach to give the parent alpha-amino acids. A minor modification to the route enabled the synthesis of Z-configured enones via the Still-Gennari reaction. A small library of Z-enones was produc

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19

Ieong, Ka-Weng. "Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids." Doctoral thesis, Uppsala universitet, Struktur- och molekylärbiologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235534.

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This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural

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20

Deboves, Herve Jean Claude. "The application of organometallic chemistry to the synthesis of new unnatural amino acids." Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324929.

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21

Monahan, Sarah Lynn Dervan Peter B. "Site-specific incorporation of unnatural amino acids into receptors expressed in mammalian cells /." Diss., Pasadena, Calif. : California Institute of Technology, 2004. http://resolver.caltech.edu/CaltechETD:etd-05252004-153512.

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22

LOCARNO, SILVIA ALICE. "UNNATURAL AMINO ACIDS AS SYNTHETIC TOOLS FOR THE PREPARATION OF COMPLEX MOLECULAR ARCHITECTURES." Doctoral thesis, Università degli Studi di Milano, 2019. http://hdl.handle.net/2434/607175.

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Self-assembly is the process by which an organized structure spontaneously is formed from individual components, as a result of specific, local interactions between the units. In recent years, peptide-based self-assembled structures have emerged as a powerful approach for developing soft and hybrid materials due to their biocompatibility, biodegradability and easily tuning properties of the final structure. The introduction of non-natural amino acids into a peptide backbone imparts additional features such as reduced conformational flexibility, high tendency to adopt a well-defined seconda

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23

Tian, Meilin. "Structure-function studies of membrane proteins by site-specific incorporation of unnatural amino acids." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066166.

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Les protéines membranaires comme les récepteurs, les canaux ioniques et les transporteurs possèdent des rôles cruciaux dans les processus biologiques tels que la signalisation physiologique et les fonctions cellulaires. La description dynamique et fonctionnelle des structures protéiques est fondamentale pour comprendre la plupart des processus concernant les macromolécules biologiques. L'incorporation, dans des protéines, d'acides aminés non naturels (Uaas) possédant des propriétés physiques ou chimiques spécifiques fournit un puissant outil pour définir la structure et la dynamique de protéin

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24

Millward, Steven Wesley Roberts Richard W. Dervan Peter B. "The design, synthesis, and evolution of macrocyclic mRNA display libraries containing unnatural amino acids /." Diss., Pasadena, Calif. : California Institute of Technology, 2007. http://resolver.caltech.edu/CaltechETD:etd-05142007-161158.

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25

Knight, William A. "Synthesis of unnatural amino acids for genetic encoding by the pyrrolysyl-tRNA/RNA synthetase system." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3794.

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The complexity of all biomolecules in existence today can be attributed to the variation of the amino acid repertoire. In nature, 20 canonical amino acids are translated to form these biomolecules, however, many of these amino acids have revealed posttranslational modifications (i.e. acetylation, methylation) after incorporation. Amino acids that exhibit PTM are known for their involvement in cellular processes such as DNA repair and DNA replication; these PTMs are commonly found on histones within the chromatin complex. Utilization of in vivo site-specific incorporation has recently reported

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26

Streichert, Katharina [Verfasser]. "Co- and posttranslational engineering of the therapeutic glycoprotein erythropoietin with unnatural amino acids / Katharina Streichert." Konstanz : Bibliothek der Universität Konstanz, 2016. http://d-nb.info/1115727133/34.

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27

Rowles, Ian. "Development of novel ammonia-lyase biocatalysts for the production of unnatural amino acids for industry." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.498954.

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28

Nguyen, Thi D. T. "Antilarval substituted phenols, distribution of tricyclic pyrones in mice, and synthesis of unnatural amino acids." Diss., Kansas State University, 2014. http://hdl.handle.net/2097/18199.

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Doctor of Philosophy<br>Department of Chemistry<br>Duy H. Hua<br>Three research projects were carried out and they are described below. The synthesis of substituted phenolic compounds including halogenated di- and trihydroxybenzenes, aminophenols, and substituted di-tert-butylphenols are described. Redox potentials of the synthesized molecules along with various known laccase substrates were measured, and an inverse relationship between the oxidation potential and the efficiency of oxidation by laccase of halogenated hydroxybenzenes and aminophenols is demonstrated. The synthesized substitu

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29

Wynn, Jessica Elaine. "Functionalizing Branched Peptides with Unnatural Amino Acids Toward Targeting HIV-1 RRE RNA and Microbials." Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/82227.

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The interaction of the protein Rev with Rev Response Element (RRE) RNA is critical to the HIV-1 life cycle as this complex is required for the export of singly-spliced and unspliced mRNAs from the nucleus to the cytoplasm. Disruption of this interaction is considered to be a powerful strategy towards the development of HIV-1 therapeutics. Therefore, we have developed several branched peptide libraries containing unnatural amino acids to target the high-affinity binding site of RRE RNA (RRE IIB), with the idea that branching in peptides can provide multivalent contacts with folded RNA structu

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30

Tang, Yi Tirrell David A. "Protein engineering using unnatural amino acids : incorporation of leucine analogs into recombinant protein in vivo /." Diss., Pasadena, Calif. : California Institute of Technology, 2002. http://resolver.caltech.edu/CaltechETD:etd-08152006-084149.

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31

Lung, Feng-Di Tiffany. "Design and synthesis of receptor-selective peptide ligands, and the synthesis of unnatural amino acids." Diss., The University of Arizona, 1995. http://hdl.handle.net/10150/187116.

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The discovery of endogenous opioid peptides has greatly accelerated research in opioid chemistry and biology. Studies of the physiological and pharmalogical roles of these receptors require highly potent and receptor-selective ligands for μ, δ, and κ receptors. The major goal of this project is to design and synthesize highly potent and κ receptor-selective dynorphin A analogues with specific conformational and topographical features. Therefore, a series of linear and cyclic dynorphin A analogues with global and/or local conformational constraints have been designed, synthesized, and evaluated

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32

Mitchell, Amanda. "Development of a Novel Genetically Encoded FRET System Using the Unnatural Amino Acid Anap." Thesis, Boston College, 2016. http://hdl.handle.net/2345/bc-ir:107177.

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Thesis advisor: Abhishek Chatterjee<br>Förster Resonance Energy Transfer (FRET) offers a powerful approach to study biomolecular dynamics in vitro as well as in vivo. The ability to apply FRET imaging to proteins in living cells provides an excellent tool to monitor important dynamic events such as protein conformational changes, protein-protein interactions, and proteolysis reactions. However, selectively incorporating two distinct fluorophores into the target protein(s) that are capable of FRET interaction within the complex cellular milieu is challenging. Consequently, terminal fusion to ge

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33

Italia, James Sebastian. "Development and Applications of Universal Genetic Code Expansion Platforms:." Thesis, Boston College, 2019. http://hdl.handle.net/2345/bc-ir:108354.

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Thesis advisor: Abhishek Chatterjee<br>The emergence of genetic code expansion (GCE) technology, which enables sitespecific incorporation of unnatural amino acids (UAAs) into proteins, has facilitated powerful new ways to probe and engineer protein structure and function. Using engineered orthogonal tRNA/aminoacyl-tRNA synthetase (aaRS) pairs that suppress repurposed nonsense codons, a variety of structurally diverse UAAs have been incorporated into proteins in living cells. This technology offers tremendous potential for deciphering the complex biology of eukaryotes, but its scope in eukaryot

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34

Rösner, Daniel [Verfasser]. "Chemical ubiquitylation of linker histone H1.2 by combining unnatural amino acids with click chemistry / Daniel Rösner." Konstanz : Bibliothek der Universität Konstanz, 2016. http://d-nb.info/1155722876/34.

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35

Marshall, Liam R. "Synthesis of unnatural amino acids for the design of multi-metal binding α-helical coiled coils". Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/19509/.

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36

Williams, Lawrence Joseph 1968. "Design and construction of organic crystals: Orthogonal recognition of pioperazinediones derived from unnatural alpha-amino acids." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282281.

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A paradigm and theoretical model for the design and construction of acentric crystalline solids is presented. In theory, molecular assembly in a predetermined arrangement will result for molecules that possess and participate in three chemically and geometrically orthogonal intermolecular interactions. A class of piperazinediones was designed to possess such orthogonal recognition elements and may provide an appropriate scaffold for the rational design of molecular building blocks for construction of acentric crystals. The topography and conformational rigidity of this scaffold limit packing o

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37

Lampkowski, Jessica S. "Development of a Solid-Supported Glaser-Hay Reaction and Utilization in Conjunction with Unnatural Amino Acids." W&M ScholarWorks, 2015. https://scholarworks.wm.edu/etd/1539626985.

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38

Kinney, William D. "Expansion of the Genetic Code to Include Acylated Lysine Derivatives and Photocaged Histidine." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5903.

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The genetic code of all known organisms is comprised of the 20 proteinogenic amino acids that serve as building blocks on a peptide chain to form a vast array of proteins. Proteins are responsible for virtually every biological process in all organisms; however, the 20 amino acids contain a limited number of functional groups that often leaves much to be desired. The lack of diversity addresses the need to increase the genetic repertoire of living cells to include a variety of amino acids with novel structural, chemical, and physical properties not found in the common 20 amino acids. In order

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39

Veljković, Ivana S. "Multicomponent reactions of siloxycyclopropanes for synthesis of unnatural amino acids and their application in synthesis of peptidomimetics." [S.l. : s.n.], 2005. http://www.diss.fu-berlin.de/2005/122/index.html.

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40

Shi, Zhengtao. "Structure-function studies of adenylate kinase by site-specific incorporation of both natural and unnatural amino acids /." The Ohio State University, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487854314871531.

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41

Qu, Hongchang. "Synthesis of Novel Amino Acids and Use of Peptides & Peptidomimetics Containing Unnatural Amino Acids for the Development of Selective Melanocortin Peptide Antagonists and for the Study of Melanocortin Receptor Signaling." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/194389.

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Unnatural amino acids are indispensible tools, not only for the elucidation of molecular mechanisms during the study of the complicated biological system, but also for the development of novel peptide and protein drugs with better efficacy and lower toxicity. Beta-substituted gamma,delta-unsaturated amino acids have been shown to be an important type of novel amino acid because of the terminal double bond which can be converted to many other functionalities. The methodology for the synthesis of syn-beta-substituted gamma,delta-unsaturated amino acids has been developed. However, there is no sa

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42

Seyedsayamdost, Mohammad R. "Investigation of the mechanism of radical propagation in E. coli ribonucleotide reductase by site-specific incorporation of unnatural amino acids." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/43089.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, February 2008.<br>Vita.<br>Includes bibliographical references.<br>Inside the cell, ribonucleotide reductases (RNRs) are responsible for the conversion of nucleotides to 2'-deoxynucleotides, an essential step in DNA biosynthesis and repair. The E. coli RNR is the best studied RNR to date and consists of two protein subunits, a2 and P2. a2 is the site of nucleotide reduction and 02 contains a diiron tyrosyl radical (Y122*) cofactor. Each turnover requires radical propagation from the Y122* in 32 to the active site of a2

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Schinn, Song Min. "Cell-Free Synthesis of Proteins with Unnatural Amino Acids: Exploring Fitness Landscapes, Engineering Membrane Proteins and Expanding the Genetic Code." BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/6496.

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Unnatural amino acids (uAA) expand the structural and functional possibilities of proteins. Numerous previous studies have demonstrated uAA as a powerful tool for protein engineering, but challenges also remain. Three notable such challenges include: (1) the fitness of uAA-incorporated proteins are difficult to predict and time-consuming to screen with conventional methods, (2) uAA incorporation in difficult-to-express proteins (e.g. membrane proteins such as G-protein coupled receptors) remain challenging, and (3) the incorporation of multiple types of uAA are still limited. In response, we p

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Cacan, Ercan. "Evolutionary synthetic biology: structure/function relationships within the protein translation system." Thesis, Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/45838.

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Production of mutant biological molecules for understanding biological principles or as therapeutic agents has gained considerable interest recently. Synthetic genes are today being widely used for production of such molecules due to the substantial decrease in the costs associated with gene synthesis technology. Along one such line, we have engineered tRNA genes in order to dissect the effects of G:U base-pairs on the accuracy of the protein translation machinery. Our results provide greater detail into the thermodynamic interactions between tRNA molecules and an Elongation Factor protein (te

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Shrestha, Prashanta. "Streamlined Extract Preparation for E. coli-Based Cell-Free Protein Synthesis and Rapid Site-Specific Incorporation of Unnatural Amino Acids in Proteins." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3917.

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This thesis reports the viability of E. coli cell extracts prepared using equipment that is both common to biotechnology laboratories and able to process small volume samples and expression of proteins containing unnatural amino acids (UAAs) at higher level using PCR amplified linear DNA templates (LETs) in cell-free protein synthesis (CFPS) system. E. coli-based cell extracts are a vital component of inexpensive and high-yielding CFPS reactions. However, effective preparation of E. coli cell extract is limited to high-pressure homogenizers (French press style or impinge-style) or bead mill ho

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Kloster, Robin A. "Indane 2,5-diketopiperazine synthons as probes of solid, solution and gas phase supramolecular non-covalent associations: Synthesis, characterization, and analysis of indane amino acids, unnatural bis-amino esters, indane 2,5-diketopiperazines." Diss., The University of Arizona, 2003. http://hdl.handle.net/10150/280299.

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Non-covalent interactions influence supramolecular organization in the solid, solution and gaseous state. While intermolecular forces are well understood individually, it is difficult to predict how their interplay will lead to a highly symmetric and low energy crystalline solid. Analysis, design, and prediction of crystalline architecture is a recently popularized sub-field of supramolecular chemistry referred to as 'crystal engineering'. Crystal engineering seeks to fully understand non-bonding intermolecular forces in order to build crystalline solids that serve some designed purpose. Hydro

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Iqbal, Emil S. "In vitro genetic code expansion and selected applications." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5580.

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The ability of incorporation non-canonical amino acids (ncAAs) using translation offers researchers the ability of extend the functionality of proteins and peptides for many applications including synthetic biology, biophysical and structural studies, and discovery of novel ligands. Here we describe the three projects where the addition of ncAAs to in vitro translation systems creates useful chemical biology techniques. In the first, a fluorinated histidine derivative is used to create a novel affinity tag that allows for the selective purification of peptides from a complex mixture of protein

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48

Bell, Stacey. "Site-directed spin-labelling of proteins for EPR spectroscopy : application to protein complexes and development of new methods for cysteine rich proteins." Thesis, University of St Andrews, 2016. http://hdl.handle.net/10023/8237.

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The work described in this thesis is an experimental study into the application of Electron Paramagnetic Resonance (EPR) Spectroscopy for the study of biological systems. Using a variety of methods of site-directed spin-labelling (SDSL), this thesis aims to explore long range structure in an assortment of recombinant and native proteins, and complexes thereof. The work described in this thesis covers all aspects of the work, from experimental design, molecular biology and cloning, protein expression and purification, as well as functional characterisation, and finally EPR distance measurements

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Gori, A. "DIFFERENT APPROACHES FOR THE PREPARATION OF PEPTIDES WITH STABILIZED PREDICTABLE SECONDARY STRUCTURE." Doctoral thesis, Università degli Studi di Milano, 2012. http://hdl.handle.net/2434/170505.

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Considering the importance of structural patterns in regulating protein-protein interactions (PPIs), the study of possible synthetic strategies to obtain the control on the preferred conformation of active biomolecules is undoubtedly a field of proven interest in contemporary science. Nonetheless, beside the modulation of molecules biological activity via structure control, a wide interest in drug discovery deals with possible ways to improve the pharmaceutical properties, e.g. metabolic stability, of defined compounds or classes of compounds. A further possibility is to combine these two sy

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50

Zhang, Wenyu. "Targeting HIV-1 RNAs with Medium Sized Branched Peptides Featuring Boron and Acridine-Branched Peptide Library Design, Synthesis, High-Throughput Screening and Validation." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/56731.

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Abstract:

RNAs have gained significant attention in recent years because they can fold into well-defined secondary or tertiary structures. These three dimensional architectures provide interfaces for specific RNA-RNA or RNA-protein interactions that are essential for biological processes in a living system. These discoveries greatly increased interest in RNA as a potential drug target for the treatment of diseases. Two of the most studied RNA based regulatory systems are HIV-1 trans-activating response element (TAR)/Tat replication pathway and Rev response element (RRE)/Rev export pathway. To efficientl

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