Log in

Relevant bibliographies by topics / UPLC–PDA / Journal articles

To see the other types of publications on this topic, follow the link: UPLC–PDA.

Journal articles on the topic 'UPLC–PDA'

Author: Grafiati

Published: 9 September 2021

Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'UPLC–PDA.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Patel, Prinesh N., D. Rajesh Kumar, S. Gananadhamu, and R. Srinivas. "Characterization of the stress degradation products of tolvaptan by UPLC-Q-TOF-MS/MS." RSC Advances 5, no. 27 (2015): 21142–52. http://dx.doi.org/10.1039/c4ra16644b.

Full text

Abstract:

TVT was subjected to forced degradation under hydrolysis, oxidation, dry heat and photolysis conditions and the degradation products (DPs) formed have been characterized through UPLC-PDA and UPLC-Q-TOF-MS/MS studies.

APA, Harvard, Vancouver, ISO, and other styles

2

Zhao, Xiangsheng, Deli Wang, Hongmei Luo, and Meihua Yang. "Simultaneous determination of three alkaloids in Huperzia serrata by UPLC-PDA and UPLC-Q/TOF-MS." Analytical Methods 7, no. 5 (2015): 1770–76. http://dx.doi.org/10.1039/c4ay02775b.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Wu, Xuejin, Lingyan Jia, Jiafan Wu, et al. "Simultaneous Determination and Quantification of Triterpene Saponins from Camellia sinensis Seeds Using UPLC-PDA-QTOF-MS/MS." Molecules 24, no. 20 (2019): 3794. http://dx.doi.org/10.3390/molecules24203794.

Full text

Abstract:

Saponins in the Camellia sinensis seeds have a broad spectrum of biological properties and application potentials. However, up to now, no chromatographic methods have been developed to provide full fingerprinting and quality assurance for these saponins. This research aimed to develop a novel method to tentatively identify and quantify saponins in C. sinensis seeds by ultra-high-performance liquid chromatography coupled with photo-diode array detector and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF-MS/MS), and compare it with the classic vanillin-sulfuric acid assay. Fifty-one triterpene saponins, including six potentially new compounds, were simultaneously detected by UPLC-PDA-MS/MS, and their chemical structures were speculated according to the retention behavior and fragmentation pattern. The total saponin content in the crude extract and the purified saponin fraction of C. sinensis seeds were quantified to be 19.57 ± 0.05% (wt %) and 41.68 ± 0.09% (wt %) respectively by UPLC-PDA at 210 nm, while the corresponding values were determined to be 43.11 ± 3.17% (wt %) and 56.60 ± 5.79% (wt %) respectively by the vanillin-sulfuric acid assay. The developed UPLC-PDA -MS/MS method could determine specified saponins, and is more reliable for quantifying the C. sinensis seed saponins than the classic spectrophotometric method. It is of great significance for the future investigations and applications of these saponins.

APA, Harvard, Vancouver, ISO, and other styles

4

Patrón-Vázquez, Jesús, Lizzie Baas-Dzul, Nelly Medina-Torres, et al. "The Effect of Drying Temperature on the Phenolic Content and Functional Behavior of Flours Obtained from Lemon Wastes." Agronomy 9, no. 9 (2019): 474. http://dx.doi.org/10.3390/agronomy9090474.

Full text

Abstract:

Lemon processing generates thousands of tons of residues that can be preserved as flours by thermal treatment to obtain phenolic compounds with beneficial bioactivities. In this study, the effect of different drying temperatures (40, 50, 60, 70, 80, 90, 100 and 110 °C) on the Total Phenolic Content (TPC), antioxidant and antimicrobial activities of phenolic compounds present in Citrus. lemon (L.) Burn f waste was determined. Identification and quantification of phenolic compounds were also performed by UPLC-PDA and UPLC-ESI-MS analysis. Eriocitrin (19.79–27.29 mg g−1 DW) and hesperidin (7.63–9.10 mg g−1 DW) were detected as the major phenolic compounds in the flours by UPLC-PDA and confirmed by UPLC-ESI-MS. Antimicrobial activity determined by Minimum Inhibitory Concentration (MIC) against Salmonella typhimurium, Escherichia coli and Staphylococcus aureus was observed. Accordingly, a stable functional flour as a source of bioactive phenolic compounds obtained from lemon residues at 50 °C may be produced as a value-added product useful in various industrial sectors.

APA, Harvard, Vancouver, ISO, and other styles

5

Wu, Zhi Jiang, and Ying Peng. "Analysis of Penicillin Antibiotics in Cosmetics Using Ultra Performance Liquid Chromatography." Advanced Materials Research 233-235 (May 2011): 87–90. http://dx.doi.org/10.4028/www.scientific.net/amr.233-235.87.

Full text

Abstract:

In this study, the utral-performance liquid chromatography (UPLC) method with UV-PDA was utilized to develop a rapid, sensitive reliable method for analysis of 5 penicillin antibiotics in cosmetics productions which the presence of these penicillin in commercial cosmetics samples is prohibited. The samples was prepared by liquid-liquid extraction and then the analyses was carried out on UPLC-PDA. The chromatographic column used was Acquity UPLCTM BEH C18 (100 mm ×2.1 mm,1.7μm) and the mobile phase is Acetonitrile and potassium dihydrogen phosphate(PBS) Buffer solution pH 5.5. Chromatographic separation was obtained by gradient elution.

APA, Harvard, Vancouver, ISO, and other styles

6

Güzel, Remziye, Zehra Ceren Ertekin, and Erdal Dinç. "A New Application of PARAFAC Model to UPLC Dataset for the Quantitative Resolution of a Tri-Component Drug Mixture." Journal of Chromatographic Science 59, no. 4 (2021): 361–70. http://dx.doi.org/10.1093/chromsci/bmaa119.

Full text

Abstract:

Abstract In the presented work, a three-way analysis of ultra-performance liquid chromatography-photodiode array (UPLC-PDA) dataset was performed by parallel factor analysis (PARAFAC) for quantitatively resolving a ternary mixture containing paracetamol and methocarbamol with indapamide selected as an internal standard in their co-eluted chromatographic conditions. Paracetamol and methocarbamol were quantified in the working range between 3–24 and 5–50 μg/mL by applying PARAFAC decomposition to UPLC-PDA data array obtained under unresolved chromatographic peak conditions. To compare the experimental results provided by co-eluted UPLC-PARAFAC method, an ordinary UPLC method was developed ensuring proper separation of the peaks. The performance of both PARAFAC and ordinary UPLC methods were assessed by quantifying independent test samples, intra- and inter-day samples and spiked samples of pharmaceutical preparations. Then, both methods were applied for quantitative estimation of the related drugs in a commercial pharmaceutical preparation. In this study, PARAFAC method was proved to be a very powerful alternative for the quality control of pharmaceutical preparations containing paracetamol and methocarbamol even in their co-eluted chromatograms with high precision and accuracy in a short chromatographic runtime of 1.2 min.

APA, Harvard, Vancouver, ISO, and other styles

7

Zhu, Fenxia, Yonggang Zhao, Xiaobin Jia, Yingjie Wei, Zhenhai Zhang, and Gulinaer Wulazihan. "Study on Fingerprint of Crude and ProcessedEpimediumby UPLC-PDA-MS." Acta Chimica Sinica 70, no. 05 (2012): 635. http://dx.doi.org/10.6023/a1105271.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Ma, Pei, Kun Luo, Yong Peng, et al. "QUALITY CONTROL OFPOLYGONUM CUSPIDATUMBY UPLC-PDA AND RELATED STATISTICAL ANALYSIS." Journal of Liquid Chromatography & Related Technologies 36, no. 20 (2013): 2844–54. http://dx.doi.org/10.1080/10826076.2012.723096.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Bairwa, Khemraj, Amit Srivastava, and Sanjay Madhukar Jachak. "Quantitative Analysis of Boeravinones in the Roots ofBoerhaavia Diffusaby UPLC/PDA." Phytochemical Analysis 25, no. 5 (2014): 415–20. http://dx.doi.org/10.1002/pca.2509.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Zhang, Shuang, Zong-ping Zheng, Mao-mao Zeng, et al. "A novel isoflavone profiling method based on UPLC-PDA-ESI-MS." Food Chemistry 219 (March 2017): 40–47. http://dx.doi.org/10.1016/j.foodchem.2016.09.120.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Ruan, Jiaqi, Zhengyue Liu, Feng Qiu, Henan Shi, and Manyuan Wang. "Simultaneous Quantification of Five Sesquiterpene Components after Ultrasound Extraction in Artemisia annua L. by an Accurate and Rapid UPLC–PDA Assay." Molecules 24, no. 8 (2019): 1530. http://dx.doi.org/10.3390/molecules24081530.

Full text

Abstract:

Objective: To develop an accurate and rapid ultra-performance liquid chromatography (UPLC) coupled with a photodiode array (PDA) method for the simultaneous determination of artemisinin (Art), arteannuin B (Art B), arteannuin C (Art C), dihydroartemisinic acid (DHAA) and artemisinic acid (AA) in Artemisia annua L. Methodology: Chromatography separation was performed on an ACQUITY UPLC BEH C18 Column with isocratic elution; the mobile phase was 0.1% formic acid aqueous solution (A) and acetonitrile (B) (A:B = 40:60, v/v). Data were recorded at an ultraviolet (UV) wavelength of 191 nm for Art, Art C, DHAA and AA, and 206 nm for Art B. Results: The calibration curves of the five sesquiterpene components were all linear with correlation coefficients more than 0.9990. The linear ranges were 31.44–1572 μg/mL, 25.48–1274 μg/mL, 40.56–2028 μg/mL, 31.44–1572 μg/mL and 26.88–1396 μg/mL for Art, Art B, Art C, DHAA and AA, respectively. The precision ranged from 0.08% to 2.88%, the stability was from 0.96% to 1.66%, and the repeatability was all within 2.42% and had a mean extraction recovery of 96.5% to 100.6%. Conclusion: The established UPLC–PDA method would be valuable for improving the quantitative analysis of sesquiterpene components in Artemisia annua L.

APA, Harvard, Vancouver, ISO, and other styles

12

Makwana, Sheetal, Madhavi Patel, Devang Prajapati, Chandni Shingala, Jatin Upadhyay, and Anamik Shah. "Validated Stability Indicating Chromatographic Method for the Simultaneous Estimation of Camylofin with NSAID Drugs and a New Approach of Method Transfer from Classical HPLC to a Modern UPLC Instrument." Chromatography Research International 2016 (October 11, 2016): 1–7. http://dx.doi.org/10.1155/2016/1596021.

Full text

Abstract:

The presented work describes the method development of simultaneous determination of camylofin dihydrochloride (CMF), diclofenac potassium (DCF), and Paracetamol (PCM) using reversed phase high performance liquid chromatography (HPLC-UV) and the method was further transferred to a new generation instrument, ultraperformance liquid chromatography (UPLC-PDA). The detailed validation was carried out for the combination tablet formulation of CMF and DCF by UPLC-PDA. From the method development study, Acquity UPLC HSS C18 (2.1 × 50 mm, 1.8 μm) was finally selected for validation. The satisfactory results were observed for peak shape, retention time, and resolution with a mobile phase of 20 mM ammonium acetate buffer (pH 3.0 with dilute orthophosphoric acid) : methanol (33 : 67 v/v). The isocratic elution of mobile phase was carried out at a flow rate of 0.250 mL/min and detection at 220 nm. Both drugs were efficiently separated out in less than 3.5 min with 1.1 and 3.2 min of retention time of the CMF and DCF with 11.87 of resolution. The linearity was obtained in the 20.0–80.0 μg/mL range of concentration with 0.9998 of correlation coefficients for the substances. The method was analyzed for specificity with detailed force degradation study, which is a simple, precise, and accurate method, as per the International Conference on Harmonization (ICH) guidelines.

APA, Harvard, Vancouver, ISO, and other styles

13

BĚLÁKOVÁ, Sylvie, Karolína BENEŠOVÁ, Renata MIKULÍKOVÁ, and Zdeněk SVOBODA. "Monitoring of changes of ferulic acid content in brewing materials using the UPLC method with PDA detector.." Kvasny Prumysl 56, no. 6 (2010): 266–69. http://dx.doi.org/10.18832/kp2010032.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Kowalska, I., D. Jedrejek, K. Jonczyk, and A. Stochmal. "UPLC–PDA–ESI–MS analysis and TLC–DPPH· activity of wheat varieties." Acta Chromatographica 31, no. 2 (2019): 151–56. http://dx.doi.org/10.1556/1326.2017.00416.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Farag, Nermeen F., Mohamed A. Farag, Enas H. Abdelrahman, Shadia M. Azzam, and El-Sayeda A. El-Kashoury. "Metabolites profiling ofChrysanthemum pacificumNakai parts using UPLC-PDA-MS coupled to chemometrics." Natural Product Research 29, no. 14 (2015): 1342–49. http://dx.doi.org/10.1080/14786419.2015.1025396.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Arathi, Bangalore Prabhashankar, Poorigali Raghavendra Rao Sowmya, Kariyappa Vijay, et al. "An Improved Method of UPLC-PDA-MS/MS Analysis of Lycopene Isomers." Food Analytical Methods 8, no. 8 (2015): 1962–69. http://dx.doi.org/10.1007/s12161-014-0083-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Troalen, Lore G., Ashley S. Phillips, David A. Peggie, Perdita E. Barran, and Alison N. Hulme. "Historical textile dyeing with Genista tinctoria L.: a comprehensive study by UPLC-MS/MS analysis." Anal. Methods 6, no. 22 (2014): 8915–23. http://dx.doi.org/10.1039/c4ay01509f.

Full text

Abstract:

A new UPLC-PDA MS/MS method has been applied to reference and historical yarns dyed with dyer's greenweed (Genista tinctoria L.). The effect of photo-degradation and textile preparation techniques (such as over-dyeing) on the dye fingerprint was investigated and the results correlated with those obtained from historical samples from the Burrell and Bodleian collections, UK.

APA, Harvard, Vancouver, ISO, and other styles

18

Ortigara, Rodolfo, Martin Steppe, and Cássia Virginia Garcia. "Stability-indicating UPLC-PDA Method for Ambrisentan Tablets and Identification of a Main Degradation Product by UPLC-MS/MS." Current Pharmaceutical Analysis 16, no. 1 (2019): 55–63. http://dx.doi.org/10.2174/1573412914666181003140449.

Full text

Abstract:

Background: Ambrisentan is a drug used to treat the pulmonary arterial hypertension symptoms, commercialized as coated tablets. Drug quality control is an essential part for the development and release of drugs for consumption; however, there are few studies related to the proposition of analytical methods and stability study for ambrisentan. Objective: The development of an UPLC assay of ambrisentan in tablets with degradation product`s elucidation was proposed. Methods: Tests with different solvents and chromatographic columns were carried out, achieving an optimal condition using mobile phase in gradient mode, Waters® BEH C18 column and detection at 260 nm. Results: Satisfactory system suitability was obtained (theoretical plates, sensitivity and resolution among peaks), with a reduced analysis time (6 minutes). The method was validated in accordance with the international guidelines and it demonstrated adequate specificity, either for the drug assay as for the identification and quantification of degradation product. It showed linearity (r= 0.999), accuracy (degradation products recovery: 98.47 - 102.44; assay recovery: 99.98 - 104.32%) and precision (RSD: 0.69), with limits of quantification and detection in suitable magnitude in order to evaluate possible drug degradation. Conclusion: UPLC method demonstrated to be fast with satisfactory robustness. The main ambrisentan degradation product formed under thermal stress conditions was elucidated by UPLC-MS/MS and its structure was suggested.

APA, Harvard, Vancouver, ISO, and other styles

19

Yin, Huihui, Xiaowen Ji, Fei Meng, et al. "Evaluation and identification of antioxidative components of Radix Rhodomyrti by DPPH–UPLC–PDA coupled with UPLC–QTOF-MS/MS." Chemical Papers 75, no. 7 (2021): 3273–81. http://dx.doi.org/10.1007/s11696-021-01544-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Xing, Z. h., W. J. Peng, W. Huang, X. Huang, and W. p. Liu. "Analysis of Major Constituents in Fructus aurantii-Magnolia Bark Decoction by UPLC-PDA." Journal of Chromatographic Science 52, no. 8 (2013): 826–30. http://dx.doi.org/10.1093/chromsci/bmt122.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Zhang, Weize, Guo Nan, Hong-Hua Wu, et al. "A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys jatamansi." Planta Medica 84, no. 08 (2017): 536–43. http://dx.doi.org/10.1055/s-0043-123655.

Full text

Abstract:

Abstract Nardostachys jatamansi is a well-documented herbal agent used to treat digestive and neuropsychiatric disorders in oriental medicinal systems. However, few simple, rapid, and comprehensive methods were reported for quality assessment and control of N. jatamansi. Herein, a UPLC with photodiode array detection method was developed for both fingerprint investigation of N. jatamansi and simultaneous quantitative analysis of the six serotonin transporter modulatory constituents in N. jatamansi. For chromatographic fingerprinting, 24 common peaks were selected as characteristic peaks to assess the consistency of N. jatamansi samples from different retail sources. Six of the common peaks (5, 7, 12, and 16 – 18) were identified as desoxo-narchinol A, buddleoside, isonardosinone, nardosinone, kanshone H, and (−)-aristolone, respectively, by phytochemical investigation. Five of the six compounds significantly either enhanced or inhibited serotonin transporter activity, while (−)-aristolone (18) didnʼt show any serotonin transporter activity. In quantitative analysis, the six compounds showed good linearity (r > 0.999) within test ranges. The precision, expressed as relative standard deviation, was in the range of 0.25 – 2.77%, and the recovery of the method was in the range of 92 – 105%. The UPLC-photodiode array detection-based fingerprint analysis and quantitative methods reported here could be used for routine quality control of N. jatamansi.

APA, Harvard, Vancouver, ISO, and other styles

22

He, Min, Hui-Ying Lv, Ya-Ping Li, et al. "A multiplex approach for the UPLC-PDA-MS/MS data: analysis of licorice." Analytical Methods 6, no. 7 (2014): 2239. http://dx.doi.org/10.1039/c3ay41861h.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Patel, A. J., N. K. Patel, A. J. Vyas, A. B. Patel, and A. I. Patel. "RP-UPLC Stability Indicating Assay Method for Simultaneous Estimation of Dextromethorphan Hydrobromide and Chlorpheniramine Maleate in Tablet Dosage Form." Asian Journal of Organic & Medicinal Chemistry 5, no. 3 (2020): 192–96. http://dx.doi.org/10.14233/ajomc.2020.ajomc-p269.

Full text

Abstract:

RP-UPLC method was developed and validated for the determination of chlorpheniramine maleate and dextromethorphan hydrobromide in tablet dosage form. Reverse phase waters acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) column using isocratic mobile phase of 0.5 mL 0.1% TFA (trifluroacetic acid) in H2O:CH3CN (70:30 %v/v). The flow rate was 0.2 mL/min and 252 nm wavelength use for detection on PDA detector. The retention time of chlorpheniramine maleate was 1.2 min and 2.2 min for dextromethorphan hydrobromide. Chlorpheniramine maleate and dextromethorphan hydrobromide was subjected to stress conditions including acidic, alkaline, oxidation, photolysis and thermal degradation. The method was validated as per ICH guideline with respect to samples to specificity, precision, accuracy, linearity and robustness.

APA, Harvard, Vancouver, ISO, and other styles

24

Боков, Дмитрий Олегович, та Ирина Александровна Самылина. "Идентификация флавоноидов настоек гомеопатических матричных Galanthus woronowii Losinsk. и Galanthus nivalis L. методом ультраэффективной жидкостной хроматографии с фотодиодноматричным и тандемным квадрупольным масс-селективным детекторами". Химико-фармацевтический журнал 50, № 7 (2016): 28–34. http://dx.doi.org/10.30906/0023-1134-2016-50-7-28-34.

Full text

Abstract:

Определён флавоноидный профиль настоек гомеопатических матричных (НГМ) 2 видов подснежника — подснежника Воронова (Galanthus woronowii Losinsk.) и подснежника белоснежного (Galanthus nivalis L.) — методом ультраэффективной жидкостной хроматографии с фотодиодноматричным и тандемным квадрупольным масс-селективным детекторами (UPLC/PDA/MS/MS). В НГМ подснежников идентифицированы флавоноловые гликозиды, агликонами которых являются кверцетин (0,075 ± 0,0047)% и изорамнетин (0,043 ± 0,0028)% в НГМ подснежника Воронова, кверцетин (0,029 ± 0,0026)% и кемпферол (0,011 ± 0,0009)% в НГМ подснежника белоснежного.

APA, Harvard, Vancouver, ISO, and other styles

25

kumar, Anshul, Chandana Majee, and Vivek Namdev. "Analytical Method development and Validation of Lamivudine in Formulation by using Reversed Phase Ultra Performance Liquid Chromatography." International Journal of PharmTech Research 13, no. 2 (2020): 1–6. http://dx.doi.org/10.20902/ijptr.2019.130201.

Full text

Abstract:

Aim of the experiment was to develop a simple, specific and accurate reverse phase ultra-performance liquid chromatographic (UPLC) method for the determination of lamivudine in the tablet dosage forms. The chromatographic separation was achieved on Acquity UPLC HSST3 (2.1 x 100mm) 1.8 um particle size and the mobile phase containing 0.1%TFA: MeOH for lamivudine. The run time was 10 min and the retention time of lamivudine was about 4.6. The detection was carried out 215nm using photo diode array detector (PDA) with a flow rate 0.6 ml/min. The linearity of lamivudine with correlation coefficient 0.9998. The recovery was found in the range (100±10%). The developed method was validated as per International Conference on Harmonization guidelines (ICH) with respect to specificity, linearity, accuracy, method precision, system precision, solution stability and robustness

APA, Harvard, Vancouver, ISO, and other styles

26

He, Wei, Maomao Zeng, Jie Chen, et al. "Identification and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in China by UPLC-PDA and UPLC-QTOF-MS/MS." Journal of Agricultural and Food Chemistry 64, no. 1 (2015): 171–77. http://dx.doi.org/10.1021/acs.jafc.5b04878.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Faqueti, Larissa G., Louis P. Sandjo, and Maique W. Biavatti. "Simultaneous identification and quantification of polymethoxyflavones, coumarin and phenolic acids in Ageratum conyzoides by UPLC-ESI-QToF-MS and UPLC-PDA." Journal of Pharmaceutical and Biomedical Analysis 145 (October 2017): 621–28. http://dx.doi.org/10.1016/j.jpba.2017.07.034.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Stój, Anna, Ireneusz Kapusta, and Dorota Domagała. "Classification of Red Wines Produced from Zweigelt and Rondo Grape Varieties Based on the Analysis of Phenolic Compounds by UPLC-PDA-MS/MS." Molecules 25, no. 6 (2020): 1342. http://dx.doi.org/10.3390/molecules25061342.

Full text

Abstract:

The authentication of grape variety from which wine is produced is necessary for protecting a consumer from adulteration and false labelling. The aim of this study was to analyze phenolic compounds in red monovarietal wines produced from Zweigelt (Vitis vinifera) and Rondo (non-Vitis vinifera) varieties while using the UPLC-PDA-MS/MS method and to assess whether these wines can be classified according to grape variety that is based on chemometric analysis. Fifty-five phenolic compounds belonging to five classes—anthocyanins, flavonols, flavan-3-ols, phenolic acids, and stilbenes—were identified and quantified in Zweigelt and Rondo wines. The wines of the Zweigelt variety were characterized by lower concentrations of phenolic compounds than those of the Rondo variety. Furthermore, wines of the Zweigelt variety contained the highest concentrations of flavan-3-ols, and wines of the Rondo variety—the highest concentrations of anthocyanins. Hierarchical cluster analysis (HCA) revealed that Zweigelt wines and Rondo wines formed two separate groups. The Rondo group was divided into two subgroups, differing in type of malolactic fermentation (spontaneous or induced). Phenolic compounds analysis by means of UPLC-PDA-MS/MS combined with HCA is a useful tool for the classification of red wines that were produced from Zweigelt and Rondo grape varieties, regardless of yeast strain and type of malolactic fermentation.

APA, Harvard, Vancouver, ISO, and other styles

29

Zhang, Lin, Chengying Wang, Dezu Miao, et al. "Simultaneous determination of six constituents in Mahuang Fuzi Xixin by UPLC-PDA–MS/MS." Natural Product Research 29, no. 8 (2014): 772–75. http://dx.doi.org/10.1080/14786419.2014.983104.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Viinamäki, J., and I. Ojanperä. "O31: Comprehensive non-MS drug screening by UPLC-PDA-CAD with durable quantitative calibration." Toxicologie Analytique et Clinique 26, no. 2 (2014): S18—S19. http://dx.doi.org/10.1016/s2352-0078(14)70039-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Bharathi, Tumkur Ramasetty, Shailasree Sekhar, Kigga Kadappa Sampath Kumara, Mudalabeedu Chandregowda Madhusudhan, and Harishchandra Sripathy Prakash. "Metabolite profiling by UPLC-PDA-ESI/HDMS and antibacterial activity of Memecylon talbotianum Brandis." Pharmacognosy Communications 6, no. 4 (2016): 225–31. http://dx.doi.org/10.5530/pc.2016.4.5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Hu, Chao, Qiande Liang, Xianglin Tang, et al. "In vivo metabolite identification of bakuchiol in rats by UPLC/ESI–PDA–QTOF–MS." Fitoterapia 106 (October 2015): 129–34. http://dx.doi.org/10.1016/j.fitote.2015.09.005.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

An, Kang, Guo Jin-rui, Zhang Zhen, and Wang Xiao-long. "Simultaneous Quantification of Ten Active Components in Traditional Chinese Formula Sijunzi Decoction Using a UPLC-PDA Method." Journal of Analytical Methods in Chemistry 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/570359.

Full text

Abstract:

Sijunzi decoction (SJZT), a traditional Chinese formula (TCMF) consisting of four herbs, has been widely used for the treatment of various gastrointestinal symptoms. However, its modernization process is hindered by the lack of a powerful quality control method that covers the major active components in the formula. The aim of this study was to establish a UPLC method for the quantitative determination of ten active components in Sijunzi decoction including ginsenoside Rg1, Re, Rb1, liquiritin, liquiritigenin, glycyrrhizic acid, atractylenolide I, atractylenolide II, atractylenolide III, and pachymic acid. Separation was achieved using an ACQUITY UPLC BEHC18column (2.1 mm × 100 mm, 1.7 μm) with a gradient elution program consisting of acetonitrile and 0.1% phosphoric acid solution. The detection wavelengths were set at 203, 254, 222, and 267 nm. The method was validated for linearity, accuracy, precision, limit of detection, and limit of quantification. The validated method was successfully applied to the simultaneous quantification of ten active compounds from several finished batches of SJZT. This validated that UPLC method is expected to provide a new basis for the quality control of SJZT.

APA, Harvard, Vancouver, ISO, and other styles

34

Ahmad, Niyaz, Rizwan Ahmad, Md Alam, and Farhan Ahmad. "Quantification and Brain Targeting of Eugenol-Loaded Surface Modified Nanoparticles Through Intranasal Route in the Treatment of Cerebral Ischemia." Drug Research 68, no. 10 (2018): 584–95. http://dx.doi.org/10.1055/a-0596-7288.

Full text

Abstract:

Abstract Objective To enhance brain bioavailability for intranasally administered Eugenol-encapsulated-chitosan-coated-PCL-Nanoparticles (CS-EUG-PCL-NPs). Methods Chitosan-coated-PCL-Nanoparticles (CS-PCL-NPs) were developed through double emulsification-solvent evaporation technique and further characterized for particle size, zeta potential, size distribution, encapsulation efficiency as well as in vitro drug release. UPLC-PDA method was developed to evaluate brain-drug uptake for optimized CS-EUG-PCL-NPs and to determine it’s pharmacokinetic in rat’s brain as well as plasma. Results Mean particles size (224.5±5.31), polydispersity index (PDI) i. e. (0.216±0.020) and entrapment efficiency (68.13±5.03) was determined for developed NPs. UPLC-PDA-eλ study showed a significantly high mucoadhesive potential of CS-EUG-PCL-NPs and least for conventional and homogenized nanoformulation; elution time for EUG and internal standard (IS) thymoquinone as 3.50 and 3.61 min were observed respectively. Furthermore, intra and inter-assay (%CV) of 0.25–1.57, %accuracy (97.11-99.00%) as well as a linear dynamic range (100.00 ng/mL–2500.0 ng/mL), was observed. Pharmacokinetic studies in Wistar rat brain and plasma exhibited a high AUC0-24 alongwith an amplified Cmax (p**<0.01) as compared to i. v. treated group. Conclusions Intranasal administration of developed CS-coated-EUG-loaded-PCL-NPs enhanced the drug bioavailability in rat brain and thus preparation of Eugenol-NPs may help treat cerebral ischemia effectively. The toxicity studies performed at the end revealed safe nature of optimized nanoformulation.

APA, Harvard, Vancouver, ISO, and other styles

35

Zhou, Shujun, Jiliang Cao, Feng Qiu, Weijun Kong, Shihai Yang, and Meihua Yang. "Simultaneous Determination of Five Bioactive Components in Radix Glycyrrhizae by Pressurised Liquid Extraction Combined with UPLC-PDA and UPLC/ESI-QTOF-MS Confirmation." Phytochemical Analysis 24, no. 6 (2013): 527–33. http://dx.doi.org/10.1002/pca.2427.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Zhao, Ziyan, Shasha He, Yan Hu, et al. "Fruit flavonoid variation between and within four cultivated Citrus species evaluated by UPLC-PDA system." Scientia Horticulturae 224 (October 2017): 93–101. http://dx.doi.org/10.1016/j.scienta.2017.05.038.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Zhu, F., J. Chen, J. Wang, R. Yin, X. Li, and X. Jia. "Qualitative and Quantitative Analysis of the Constituents in Danmu Preparations by UPLC-PDA-TOF-MS." Journal of Chromatographic Science 52, no. 8 (2013): 862–71. http://dx.doi.org/10.1093/chromsci/bmt129.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Farag, Mohamed A., Mohamed S. Abdelfattah, Sherif E. A. Badr, and Ludger A. Wessjohann. "Profiling the chemical content of Ficus lyrata extracts via UPLC-PDA-qTOF-MS and chemometrics." Natural Product Research 28, no. 19 (2014): 1549–56. http://dx.doi.org/10.1080/14786419.2014.926353.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Farag, Mohamed A., Aisha H. Abou Zeid, Manal A. Hamed, Zeinab Kandeel, Hanaa M. El-Rafie, and Radwa H. El-Akad. "Metabolomic fingerprint classification of Brachychiton acerifolius organs via UPLC-qTOF-PDA-MS analysis and chemometrics." Natural Product Research 29, no. 2 (2014): 116–24. http://dx.doi.org/10.1080/14786419.2014.964710.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Alajmi, Mohamed Fahad, Perwez Alam, Md Tabish Rehman, et al. "Interspecies Anticancer and Antimicrobial Activities of GenusSolanumand Estimation of Rutin by Validated UPLC-PDA Method." Evidence-Based Complementary and Alternative Medicine 2018 (July 3, 2018): 1–13. http://dx.doi.org/10.1155/2018/6040815.

Full text

Abstract:

Solanaceae is one of the highly diverse plant families of whichSolanumis the largest genera (1700 species) containing several pharmacological properties like anticancer and antimicrobial. This motivated us to explore the anticancer (against HepG2, HEK-293, and MCF-7 cells) and antimicrobial (againstS. aureus, E. coli, P. aeruginosa,andC. albicans) properties ofS. schimperianum,S. villosum,S. coagulans,S. glabratum,S. incanum,andS. nigrumalong with rutin estimation by UPLC-PDA method. Of the studiedSolanumextracts,S. nigrumexhibited significant cytotoxic property against HepG2 (IC50: 20.4μg/mL) and MCF-7 (IC50: 30.1μg/mL);S. coagulansshowed toxicity against HepG2 (IC50: 28.4μg/mL) and HEK-293 cells (IC50: 25.7μg/mL) compared to 5-Fluorouracil (standard). Compared to these, extracts ofS. coagulansandS. glabratumexhibited relatively high antimicrobial potency (MIC: 0.4-1.6 mg/mL). Nonetheless, allSolanumextracts significantly reduced the biofilm against PAO1-strain. Rutin was detected in all extracts with the highest content (53.79μg/mg) inS. coagulansthat supported its strong antimicrobial and anticancer properties. Molecular docking analysis showing strong binding of rutin with human DNA and proteins (DNA Topoisomerase IIαandE. coliDNA gyrase B) supported the anticancer and antimicrobial activities ofSolanumspecies.

APA, Harvard, Vancouver, ISO, and other styles

41

Iram, Farah, Perwez Alam, Nasir A. Siddiqui, et al. "Development of a stress induced validated UPLC-PDA method for the analysis of Eslicarbazepine acetate." Saudi Pharmaceutical Journal 26, no. 2 (2018): 286–91. http://dx.doi.org/10.1016/j.jsps.2017.11.009.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Rubert‐Nason, Kennedy F., and Richard L. Lindroth. "Analysis of condensed tannins inPopulusspp. using reversed phase UPLC‐PDA‐(−)esi‐MS following thiolytic depolymerisation." Phytochemical Analysis 30, no. 3 (2018): 257–67. http://dx.doi.org/10.1002/pca.2810.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Tao, Wei-Wei, Jin-Ao Duan, Nian-Yun Yang, et al. "Determination of Nucleosides and Nucleobases in the Pollen of Typha Angustifolia by UPLC-PDA-MS." Phytochemical Analysis 23, no. 4 (2011): 373–78. http://dx.doi.org/10.1002/pca.1367.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Liao, Shang-Gao, Yue-Ting Li, Li-Juan Zhang, et al. "UPLC-PDA-ESI-MS/MS Analysis of Compounds Extracted by Cardiac h9c2 Cell fromPolygonum orientale." Phytochemical Analysis 24, no. 1 (2012): 25–35. http://dx.doi.org/10.1002/pca.2374.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Farag, Mohamed A., Mohamed G. Sharaf Eldin, Hanaa Kassem, and Mohamed Abou el Fetouh. "Metabolome Classification ofBrassica napusL. Organs via UPLC-QTOF-PDA-MS and Their Anti-oxidant Potential." Phytochemical Analysis 24, no. 3 (2012): 277–87. http://dx.doi.org/10.1002/pca.2408.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Fattahi, Azin, Abolfazl Shakeri, Zahra Tayarani‐Najaran, et al. "UPLC–PDA‐ESI–QTOF–MS/MS and GC‐MS analysis of Iranian Dracocephalum moldavica L." Food Science & Nutrition 9, no. 8 (2021): 4278–86. http://dx.doi.org/10.1002/fsn3.2396.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Patel, D. M., B. Rana, S. Maru, et al. "Stability Indicating RP-UPLC Photo-Diode Array based Method for Determination of Edoxaban Tosylate." Asian Journal of Organic & Medicinal Chemistry 5, no. 3 (2020): 273–76. http://dx.doi.org/10.14233/ajomc.2020.ajomc-p270.

Full text

Abstract:

The objective of the current study was to develop a specific, precise, accurate and robust gradient stability indicating reversed-phase ultra performance liquid chromatography (RP-UPLC-PDA) assay method and validated for determination of edoxaban tosylate in API. Gradient separation was achieved on an acquity UPLC BEH C18 column (50 mm, 2.1 mm and 1.7 μm) column using mobile phase of acetoitrile:20 mM potassium dihydrogen phosphate, pH 3.0 ± 0.05 adjust with OPA at flow rate of 0.6 mL/min, the injection volume was 1 μL and the detection was carried out of 289 nm by using photodiode array detector. The drug was subjected to oxidation, hydrolysis, photolysis, and heat to apply stress condition. The method was linear in the drug concentration range of 100-300 μg/mL with correlation coefficient of 0.999. Degradation products produced as a result of stress studies did not interfere with detection of edoxaban tosylate and the assay, thus developed stability indicating method can be used for routine analysis in pharmaceutical industry.

APA, Harvard, Vancouver, ISO, and other styles

48

Oszmiański, Jan, Sabina Lachowicz, Paulina Nowicka, Paweł Rubiński, and Tomasz Cebulak. "Evaluation of Innovative Dried Purée from Jerusalem Artichoke—In Vitro Studies of Its Physicochemical and Health-Promoting Properties." Molecules 26, no. 9 (2021): 2644. http://dx.doi.org/10.3390/molecules26092644.

Full text

Abstract:

The present study aimed to evaluate the effect of Jerusalem artichoke processing methods and drying methods (freeze drying, sublimation drying, vacuum drying) on the basic physicochemical parameters, profiles and contents of sugars and polyphenolic compounds, and health-promoting properties (antioxidant activity, inhibition of the activities of α-amylase, α-glucosidase, and pancreatic lipase) of the produced purée. A total of 25 polyphenolic compounds belonging to hydroxycinnamic phenolic acids (LC-PDA-MS-QTof) were detected in Jerusalem artichoke purée. Their average content in the raw material was at 820 mg/100 g dm (UPLC-PDA-FL) and was 2.7 times higher than in the cooked material. The chemical composition and the health-promoting value of the purées were affected by the drying method, with the most beneficial values of the evaluated parameters obtained upon freeze drying. Vacuum drying could offer an alternative to freeze drying, as both methods ensured relatively comparable values of the assessed parameters.

APA, Harvard, Vancouver, ISO, and other styles

49

Charbe, Nitin B., Flavia C. Zacconi, Nikhil Amnerkar, B. Ramesh, Murtaza M. Tambuwala, and Emilio Clementi. "Bio-analytical Assay Methods used in Therapeutic Drug Monitoring of Antiretroviral Drugs-A Review." Current Drug Therapy 14, no. 1 (2019): 16–57. http://dx.doi.org/10.2174/1574885514666181217125550.

Full text

Abstract:

Background: Several clinical trials, as well as observational statistics, have exhibited that the advantages of antiretroviral [ARV] treatment for humans with Human Immunodeficiency Virus / Acquired Immune Deficiency Syndrome HIV/AIDS exceed their risks. Therapeutic drug monitoring [TDM] plays a key role in optimization of ARV therapy. Determination of ARV’s in plasma, blood cells, and other biological matrices frequently requires separation techniques capable of high effectiveness, specific selectivity and high sensitivity. High-performance liquid chromatography [HPLC] coupled with ultraviolet [UV], Photodiode array detectors [PDA], Mass spectrophotometer [MS] detectors etc. are the important quantitative techniques used for the estimation of pharmaceuticals in biological samples. </P><P> Objective: This review article is aimed to give an extensive outline of different bio-analytical techniques which have been reported for direct quantitation of ARV’s. This article aimed to establish an efficient role played by the TDM in the optimum therapeutic outcome of the ARV treatment. It also focused on establishing the prominent role played by the separation techniques like HPLC and UPLC along with the detectors like UV and Mass in TDM. </P><P> Methods: TDM is based on the principle that for certain drugs, a close relationship exists between the plasma level of the drug and its clinical effect. TDM is of no value if the relationship does not exist. The analytical methodology employed in TDM should: 1) distinguish similar compounds; 2) be sensitive and precise and 3) is easy to use. </P><P> Results: This review highlights the advancement of the chromatographic techniques beginning from the HPLC-UV to the more advanced technique like UPLC-MS/MS. TDM is essential to ensure adherence, observe viral resistance and to personalize ARV dose regimens. It is observed that the analytical methods like immunoassays and liquid chromatography with detectors like UV, PDA, Florescent, MS, MS/MS and Ultra performance liquid chromatography (UPLC)-MS/MS have immensely contributed to the clinical outcome of the ARV therapy. Assay methods are not only helping physicians in limiting the side effects and drug interactions but also assisting in monitoring patient’s compliance. </P><P> Conclusion: The present review revealed that HPLC has been the most widely used system irrespective of the availability of more sensitive chromatographic technique like UPLC.

APA, Harvard, Vancouver, ISO, and other styles

50

Dubey, Somshankar, and Mahesh Duggirala. "SIMULTANEOUS ESTIMATION OF LAMIVUDINE, ABACAVIR AND DOLUTEGRAVIR BY UPLC METHOD." International Journal of Applied Pharmaceutics 10, no. 1 (2018): 46. http://dx.doi.org/10.22159/ijap.2018v10i1.21156.

Full text

Abstract:

Objective: To develop a simple, rapid, sensitive and effective reverse phase ultra-performance liquid chromatographic method (RP-UPLC) for simultaneous quantification of lamivudine, abacavir and dolutegravir in pure and tablet dosage forms.Methods: Chromatographic separation was performed by using Waters-ACQUITY UPLC system equipped with auto sampler, photodiode array (PDA) detector, zodiac sil RP C18 (4.6 mm × 250 mm, 3.0 µm) column, phosphate buffer (pH 3.0) and methanol in the ratio of 30:70 %v/v have been delivered at a flow rate of 0.25 ml/min and the detection was carried out using a Ultraviolet (UV) detector at a wavelength of 260 nm at ambient column temperature. The mobile phase was used as diluent.Results: The retention time (Rt) for lamivudine, abacavir and dolutegravir were 1.763, 2.247 and 3.175 min respectively. A good linear response was obtained in the range of 15-75 µg/ml, 30-150 µg/ml and 2.5-12.5 µg/ml, respectively. The Limit of Detection (LOD) values were found to be 0.021, 0.330 and 0.038 µg/ml, respectively and the Limit of Quantitation (LOQ) values were 0.056, 1.320 and 0.095 µg/ml, respectively.Conclusion: It was concluded that the developed RP-UPLC method was effective, suitable and conducive for analyzing lamivudine, abacavir and dolutegravir in pharmaceutical formulations. The method was quantitatively evaluated in terms of precision, linearity, accuracy (recovery), selectivity and robustness in accordance with standard guidelines.

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!