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Journal articles on the topic 'UPLC'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Neeraja, T., Sridevi Pingali, Vinutha K, and Bhagavan Raju M. "UPLC: A MODERN ANALYTICALTECHNIQUE." Indian Research Journal of Pharmacy and Science 4, no. 4 (2017): 1171–81. http://dx.doi.org/10.21276/irjps.2017.4.4.2.

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Zhu, Yanqin, Qinhong Yin, and Yaling Yang. "A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves." Tropical Journal of Pharmaceutical Research 20, no. 11 (2021): 2371–79. http://dx.doi.org/10.4314/tjpr.v20i11.20.

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Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as t

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Ichsan, Dewi Susetiyany, and Ma'rifah Ebtasari. "Identifikasi Efedrin dan Pseudoefedrin Sediaan Padat dengan Metode Ultra Performance Liquid Chromatography dan High Performance Liquid Chromatography." Eruditio : Indonesia Journal of Food and Drug Safety 3, no. 2 (2023): 99–106. http://dx.doi.org/10.54384/eruditio.v3i2.130.

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Ultra Performance Liquid Chromatography (UPLC) adalah pemisahan analitik yang mempertahankan kepraktisan dan prinsip High Performance Liquid Chromatography (HPLC) dengan meningkatkan kecepatan analisis, sensitivitas, dan resolusi. Selama ini metode yang digunakan untuk identifikasi efedrin dan pseudoefedrin dilakukan menggunakan HPLC sesuai MA PPOMN 07/NA/2010 dari Pusat Pengembangan Pengujian Obat dan Makanan Nasional (PPPOMN). Tujuan penelitian untuk menilai keunggulan metode UPLC dibandingkan dengan HPLC pada identifikasi Efedrin dan Pseudoefedrin sediaan padat. UPLC yang digunakan UPLC Wat

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Jebaliya, Hetal, Madhavi Patel, Yashwant Jadeja, Batuk Dabhi, and Anamik Shah. "A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study." Chromatography Research International 2013 (December 4, 2013): 1–5. http://dx.doi.org/10.1155/2013/673150.

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The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μm particle size) column used for UPLC. The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC. Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performe

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G, Patil Vedashri. "A Review on Ultra High Performance liquid Chromatography Method." International Journal for Research in Applied Science and Engineering Technology 11, no. 6 (2023): 311–19. http://dx.doi.org/10.22214/ijraset.2023.53541.

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Abstract: UPLC is a modern technique which gives a new direction for liquid Chromatography. UPLC refers to ultra performance liquid chromatography, Which enhance mainly in three areas: “speed, resolution and sensitivity. Ultra Performance liquid chromatography (UPLC) applicable for particle less than 2µm in diameter to acquire better resolution, speed, and sensitivity compared With high-performance liquid chromatography (HPLC). In twenty first centenary Pharmaceutical industries are focusing for new ways to in economy and shorten Time for development of drugs. UPLC analysis at the mean time gi

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Gorja, Ashok, Preethi Medala, M. Madhavi, and Gajjagouni Meghana goud. "A Review on Ultra Performance Liquid Chromatography." Saudi Journal of Medical and Pharmaceutical Sciences 11, no. 04 (2025): 279–86. https://doi.org/10.36348/sjmps.2025.v11i04.007.

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UPLC is a modern technique which gives a new direction for liquid Chromatography. UPLC refers to ultra performance liquid chromatography, which enhance mainly in three areas: “speed, resolution, and sensitivity. Ultra Performance liquid chromatography (UPLC) applicable for particles less than 2µm in diameter to acquire better resolution, speed, and sensitivity compared with high-performance liquid chromatography (HPLC). In twenty first centenary pharmaceutical industries are focusing for new ways to in economy and shorten Time for development of drugs. UPLC analysis at the meantime gives the b

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Kiran, Gaikwad*1 Vishal Madankar2. "Ultra Performance Liquid Chromatography (Uplc): A New Trend in Analysis." International Journal of Scientific Research and Technology 2, no. 3 (2025): 458–67. https://doi.org/10.5281/zenodo.15082659.

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Ultra Performance Liquid Chromatography (UPLC) is a modern analytical technique widely utilized in analytical chemistry and the pharmaceutical sector. It operates on the principles of column chromatography, which is typically employed to analyze mixtures. UPLC offers innovative advancements in liquid chromatography, allowing for shorter columns, reduced analysis time, and lower solvent usage. By utilizing particles smaller than 2µm, it significantly improves the speed, resolution, and sensitivity of analyses. Ultra Performance Liquid Chromatography (UPLC) leverages advancements in partic

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khan, Pathan Daud Ahmed. "A Comprehensive Review on Advantages of Ultra Performance Liquid Chromatography (UPLC) for Analytical Method Development, Validation, and Stability Studies." INTERANTIONAL JOURNAL OF SCIENTIFIC RESEARCH IN ENGINEERING AND MANAGEMENT 08, no. 05 (2024): 1–5. http://dx.doi.org/10.55041/ijsrem34104.

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Ultra Performance Liquid Chromatography (UPLC) represents a paradigm shift in analytical separation science, offering enhanced speed, sensitivity, and resolution while retaining the foundational principles of traditional liquid chromatography. This comprehensive review explores the principles, instrumentation, advantages, and applications of UPLC in analytical method development, validation, and stability studies, with a focus on pharmaceutical analysis. UPLC leverages finer particles and intensified flow rates to achieve unprecedented performance gains, facilitating rapid and precise separati

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9

Ismail Y and Jayadev Seelam. "Ultra-Performance Liquid Chromatography (UPLC) in Analytical Method Development and Validation Following In-vitro Studies." Journal of Pharma Insights and Research 3, no. 2 (2025): 082–89. https://doi.org/10.69613/rwktfg11.

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Ultra-performance liquid chromatography (UPLC) has emerged as a powerful analytical tool, offering superior resolution, sensitivity, and rapid analysis compared to conventional high-performance liquid chromatography. The integration of UPLC with advanced detection systems, particularly mass spectrometry, has transformed pharmaceutical research, biomarker discovery, and metabolomics studies. Recent innovations in column technology, including sub-2-micron particles and core-shell materials, have significantly enhanced separation efficiency and detection limits. UPLC plays a crucial role in drug

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Zhao, Xiangsheng, Liwei Xie, Haifeng Wu, Weijun Kong, and Meihua Yang. "Analysis of six bioactive components in Semen Ziziphi Spinosae by UPLC-ELSD and UPLC-Q/TOF-MS." Anal. Methods 6, no. 15 (2014): 5856–64. http://dx.doi.org/10.1039/c4ay00800f.

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Rab, Rehan Abdur, Sultan Zahiruddin, Mohammad Ibrahim, et al. "HPTLC and UPLC-MS/MS Methods for Quality Control Analysis of Itrifal Formulations of Unani System of Medicine." Journal of AOAC INTERNATIONAL 103, no. 3 (2020): 649–58. http://dx.doi.org/10.5740/jaoacint.19-0231.

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Abstract Background: Ultra-performance LC (UPLC)–tandem MS (MS/MS) and high-performance TLC (HPTLC) assay methods were developed for chemical fingerprinting and quantitative analysis of bioactive constituents of a certain “Itrifal formulation,” a traditional kind of Unani medicine. Objective: In the present investigation, HPTLC and UPLC-MS/MS methods were developed and validated for the detection and quantification of major metabolites present in itrifal formulation. Methods: The metabolites present in the formulation were separated using modern chromatographic techniques, and a quantitative a

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Dinç, Erdal, and Eda Büker. "A New Application of Continuous Wavelet Transform to Overlapping Chromatograms for the Quantitative Analysis of Amiloride Hydrochloride and Hydrochlorothiazide in Tablets by Ultra-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 95, no. 3 (2012): 751–56. http://dx.doi.org/10.5740/jaoacint.sge_dinc.

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Abstract A new application of continuous wavelet transform (CWT) to overlapping peaks in a chromatogram was developed for the quantitative analysis of amiloride hydrochloride (AML) and hydrochlorothiazide (HCT) in tablets. Chromatographic analysis was done by using an ACQUITY ultra-performance LC (UPLC) BEH C18 column (50 × 2.1 mm id, 1.7 μm particle size) and a mobile phase consisting of methanol–0.1 M acetic acid (21 + 79, v/v) at a constant flow rate of 0.3 mL/min with diode array detection at 274 nm. The overlapping chromatographic peaks of the calibration set consisting of AML and HCT mix

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Rajendra, Kalamkar Sahil Pathan Santosh Bade. "A Systematic Review On UPLC." International Journal in Pharmaceutical Sciences 2, no. 6 (2024): 1267–75. https://doi.org/10.5281/zenodo.12571455.

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Ultra Performance Liquid Chromatography (UPLC) can be regarded as a new direction for liquid chromatography. UPLC improves three areas of Liquid chromatography: speed, resolution, and sensitivity. In this system column containing bridged ethyl siloxane / silica hybrid (BEH) structure with fine particle size (less than 1.7µm) is utilized. The use of which decreases the length of column, saves time and reduces solvent consumption. This system is designed in a special way to withstand high system back-pressures. Now a day’s pharmaceutical industries as well as analytical laboratories

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Kapil, L. Dubal, R. Ram Vijay, J. Kher Govind, N. Dave Pragnesh, S. Joshi Hitendra, and Khosla Ekta. "Comparison of Throat Sprays Containing Chlorhexidine Gluconate and Lidocaine Hydrochloride." International Journal of Current Pharmaceutical Review and Research 7, no. 3 (2016): 141–50. https://doi.org/10.5281/zenodo.12681079.

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A novel UPLC method for determination of chlorhexidine gluconate and lidocaine hydrochloride in throat sprays hasbeen presented as a tool suitable for quality monitoring of these compounds. The developed and validated UPLC methodwas compared with HPLC method. Experimental parameters like analysis time, sensitivity, mobile phase consumption,validation data, and demands on instrument equipment and operation were compared for two methods. UPLC and HPLCare important methods for routine monitoring of these drugs. In UPLC, Major degradation was found in photolyticcondition that product was degraded

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Siddiqui, Khushnuma, and Bhavana Dubey. "Analytical Method Development and Validation of Ondansetron and Telmisartan in Tablet Dosage Form by RP-UPLC Method." Journal of Drug Delivery and Therapeutics 12, no. 4-S (2022): 112–27. http://dx.doi.org/10.22270/jddt.v12i4-s.5548.

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The objective of the present analysis was the improvement of easy, exact as well as reproducible and precise verifies reversed phase Liquid Chromatography with High Performance [UPLC] assays for Telmisartan and Ondansetron. The chromatographic splitting up was done on Acuity UPLC BEH C 18 (50×2.1mm and1.7µm) column with gradient elution. The injection amount was 1µl and also the detection was performed at 214 nm by utilizing photo diode array detector. The evolved as well as a validated UPLC way of Telmisartan and Ondansetron in tablet dosage styles based on ICH tips was precise and accurate,

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Patel, Prinesh N., D. Rajesh Kumar, S. Gananadhamu, and R. Srinivas. "Characterization of the stress degradation products of tolvaptan by UPLC-Q-TOF-MS/MS." RSC Advances 5, no. 27 (2015): 21142–52. http://dx.doi.org/10.1039/c4ra16644b.

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TVT was subjected to forced degradation under hydrolysis, oxidation, dry heat and photolysis conditions and the degradation products (DPs) formed have been characterized through UPLC-PDA and UPLC-Q-TOF-MS/MS studies.

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Rochat, Bertrand, Andres Pascual, Benoît Pesse, et al. "Ultra-Performance Liquid Chromatography Mass Spectrometry and Sensitive Bioassay Methods for Quantification of Posaconazole Plasma Concentrations after Oral Dosing." Antimicrobial Agents and Chemotherapy 54, no. 12 (2010): 5074–81. http://dx.doi.org/10.1128/aac.00022-10.

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ABSTRACT Posaconazole (POS) is a new antifungal agent for prevention and therapy of mycoses in immunocompromised patients. Variable POS pharmacokinetics after oral dosing may influence efficacy: a trough threshold of 0.5 μg/ml has been recently proposed. Measurement of POS plasma concentrations by complex chromatographic techniques may thus contribute to optimize prevention and management of life-threatening infections. No microbiological analytical method is available. The objective of this study was to develop and validate a new simplified ultra-performance liquid chromatography tandem mass

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Wang, Bo, Jianyu Liu, Xia Zhao, et al. "Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry." Molecules 25, no. 4 (2020): 987. http://dx.doi.org/10.3390/molecules25040987.

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A method for the simultaneous determination of robenidine, halofuginone, lasalocid, monensin, nigericin, salinomycin, narasin, and maduramicin residues in eggs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed. The sample preparation method used a combination of liquid–liquid extraction (LLE) and solid-phase extraction (SPE) technology to extract and purify these target compounds from eggs. The target compounds were separated by gradient elution using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). Tandem mass spectrome

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Güzel, Remziye, Zehra Ceren Ertekin, and Erdal Dinç. "A New Application of PARAFAC Model to UPLC Dataset for the Quantitative Resolution of a Tri-Component Drug Mixture." Journal of Chromatographic Science 59, no. 4 (2021): 361–70. http://dx.doi.org/10.1093/chromsci/bmaa119.

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Abstract In the presented work, a three-way analysis of ultra-performance liquid chromatography-photodiode array (UPLC-PDA) dataset was performed by parallel factor analysis (PARAFAC) for quantitatively resolving a ternary mixture containing paracetamol and methocarbamol with indapamide selected as an internal standard in their co-eluted chromatographic conditions. Paracetamol and methocarbamol were quantified in the working range between 3–24 and 5–50 μg/mL by applying PARAFAC decomposition to UPLC-PDA data array obtained under unresolved chromatographic peak conditions. To compare the experi

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Chernova, Alina, Pavel Mazin, Svetlana Goryunova, et al. "Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops." PeerJ 7 (March 6, 2019): e6547. http://dx.doi.org/10.7717/peerj.6547.

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Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID tec

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Santhakumari, K., S. Mohan, and K. Prasada Rao. "Validated Method Development of Levamisole and Inosine pranobex by using UPLC &Characterization of Degradants by LC-MS/MS." YMER Digital 21, no. 07 (2022): 734–44. http://dx.doi.org/10.37896/ymer21.07/59.

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In order to simultaneously evaluate Inosine pranobex and levamisole in pharmaceutical formulations using UPLC, a reliable and simple method was developed. The chromatographic separation used in this method a Phenomenox C18 column (50mmx2.1mm, 3.5). % Trifluoroacetic Acid (TFA) and Methanol were employed in a mobile phase with a flow rate of 0.5 mL/min and room temperature. At 223 nm, a UV observation was made. With these settings, we were able to successfully use UPLC to achieve good linearity throughout a range of 12.5-75 g/ml of inosine pranobex and 2.5-15 g/mL of levamisole. The results of

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Stegmüller, Simone, Nadine Beißmann, Jonathan Isaak Kremer, Denise Mehl, Christian Baumann, and Elke Richling. "A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption." Molecules 25, no. 21 (2020): 5104. http://dx.doi.org/10.3390/molecules25215104.

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We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously rep

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Jin, Peng, Kai Yang, Ruining Bai, et al. "Development and comparison of UPLC-ESI-MS and RP-HPLC-VWD methods for determining microcystin-LR." RSC Advances 11, no. 37 (2021): 23002–9. http://dx.doi.org/10.1039/d1ra03521e.

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UPLC-MS and HPLC-VWD methods were used together for MC-LR determination in a wide concentration range. UPLC-MS can be applied in trace MC-LR determination, whereas HPLC-VWD is more suitable for high concentration range detection.

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Siva Madhu Chaitanya, Srinath Nissankararao, and Satya Lakshmi Gandham. "A sort of validated Bioanalytical method developed for the estimation of Etoposide and Cisplatin in rat plasma by using two different advanced liquid chromatographic techniques like HPLC and UPLC and its application in bioequivalence studies." International Journal of Research in Pharmaceutical Sciences 12, no. 1 (2021): 708–17. http://dx.doi.org/10.26452/ijrps.v12i1.4167.

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An easy, quick, precise, active and reproducible reverse method high performance liquid chromatography (RP-HPLC), as well as Ultra pressure liquid chromatography (UPLC) unique technique, was developed for the bioanalytical method of Etoposide and Cisplatin with Oxaplatin as an internal standard. Chromatographic analysis was performed using HPLC was Waters Alliance e-2695, and UPLC was Waters Acquity UPLC by using x-bridge phenyl 150x4.6mm, 3.5µ column and therefore the mobile phase containing 0.1% triethylamine and acetonitrile at a ratio out of 60:40 v/v. The flow rate was 1 ml/min, and analy

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Dr., G. Nagaraju, Teena Lavdya, Sirisha V., and Hareesh Dara Dr. "DETERMINATION OF ANTIPSYCHOTIC MEDICATIONS IN HUMAN PLASMA BY UPLC: PHARMACOKINETIC STUDY." Journal of Pharma Research 12, no. 6 (2024): 64–69. https://doi.org/10.5281/zenodo.14233951.

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<strong>Abstract</strong> <strong>A</strong> simple and sensitive ultra-performance liquid chromatography (UPLC) method has been developed and validated for simultaneous estimation of olanzapine (OLZ), risperidone (RIS) and 9-hydroxyrisperidone (9-OHRIS) in human plasma in vitro. The sample preparation was performed by simple liquid–liquid extraction technique. The analytes were chromatographed on a Waters Acquity H class UPLC system using isocratic mobile phase conditions at a flow rate of 0.3mL/min and Acquity UPLC BEH shield RP18 column maintained at 40°C. Quantification was perfo

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Patrón-Vázquez, Jesús, Lizzie Baas-Dzul, Nelly Medina-Torres, et al. "The Effect of Drying Temperature on the Phenolic Content and Functional Behavior of Flours Obtained from Lemon Wastes." Agronomy 9, no. 9 (2019): 474. http://dx.doi.org/10.3390/agronomy9090474.

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Lemon processing generates thousands of tons of residues that can be preserved as flours by thermal treatment to obtain phenolic compounds with beneficial bioactivities. In this study, the effect of different drying temperatures (40, 50, 60, 70, 80, 90, 100 and 110 °C) on the Total Phenolic Content (TPC), antioxidant and antimicrobial activities of phenolic compounds present in Citrus. lemon (L.) Burn f waste was determined. Identification and quantification of phenolic compounds were also performed by UPLC-PDA and UPLC-ESI-MS analysis. Eriocitrin (19.79–27.29 mg g−1 DW) and hesperidin (7.63–9

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Sharma, Arti, Ritika Sharma, Rohit Arora, Saroj Arora, Bikram Singh, and Upendra Sharma. "Quantitative and Qualitative Analysis of Eruca sativa and Brassica juncea Seeds by UPLC-DAD and UPLC-ESI-QTOF." Natural Product Communications 12, no. 9 (2017): 1934578X1701200. http://dx.doi.org/10.1177/1934578x1701200927.

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Eruca sativa and Brassica juncea belong to the Brassicaceae family and have been used traditionally for the treatment of cancer and various cardiovascular ailments. A rapid and simple ultra performance liquid chromatography (UPLC) method has been developed for the simultaneous quantification of erucin, allyl isothiocyanate and benzyl isothiocyanate in E. sativa and B. juncea oil. Eruca sativa oil contains erucin (28.93%), allyl isothiocyanate (0.06%) and benzyl isothiocyanate (0.70%). Identification of other secondary metabolites in E. sativa and B. juncea oil and aqueous methanolic extracts w

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Chawla, Gita, and Chanda Ranjan. "Principle, Instrumentation, and Applications of UPLC: A Novel Technique of Liquid Chromatography." Open Chemistry Journal 3, no. 1 (2016): 1–16. http://dx.doi.org/10.2174/1874842201603010001.

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The key focus of the pharmaceutical or chemical industries is to reduce the cost involved in the development of new drugs and to improve the selectivity, sensitivity, and resolution for their detection. The purpose can now be solved by the separation method called UPLC which is the modified HPLC method comprising high pressure and small sized particles (less than 2 µm) used in the column, so the length of the column decreases leading to time saving and reduction in the consumption of solvent. The underlying principle of UPLC is based on van Deemter statement which describes the connection betw

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Shi, Yurui, Rongda Chen, Jing Xie, et al. "Determination and Pharmacokinetics of Okanin in Rat Plasma by UltraHigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Tandem Mass Spectrometry." Journal of Analytical Methods in Chemistry 2020 (August 28, 2020): 1–7. http://dx.doi.org/10.1155/2020/4247128.

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Okanin is a major flavonoid found in Coreopsis tinctoria Nutt., arousing huge interest recently for its considerable biological characteristics including antioxidant, antineurotoxic, and antidiabetic activities. An ultrahigh performance liquid chromatography triple-quadrupole tandem mass spectrometry (UPLC-MS) was successfully used to determine okanin in rat plasma after oral administration of okanin. Bavachalcone acted as an internal standard (IS). By gradient elution, IS and analyte were separated on a C18 column for 7 min at a flow rate of 0.25 mL/min with acetonitrile-0.1% acetic acid mobi

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Cason, Chevelle A., Stuart A. Oehrle, Thomas A. Fabré, et al. "Improved Methodology for Monitoring Poly(amidoamine) Dendrimers Surface Transformations and Product Quality by Ultra Performance Liquid Chromatography." Journal of Nanomaterials 2008 (2008): 1–7. http://dx.doi.org/10.1155/2008/456082.

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Ultra performance liquid chromatography (UPLC) analysis was utilized for the first time as a methodology for monitoring poly(amidoamine) (PAMAM) dendrimer surface transformations and product quality. Results were compared to high-performance liquid chromatography (HPLC) and were found to provide a vastly improved analytical method for the characterization of dendrimer polydispersity and variance in a typical surface modification. The application of UPLC increased the average number of theoretical plates by a factor of 7 and reduced retention times of analytes by 36%, while improving the resolu

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Nandan, K. Dhir, and K. Yadav S. "Estimation of Clotrimazole residue in swab samples via validated UPLC method." Journal of Indian Chemical Society Vol. 89, Dec 2012 (2012): 1665–70. https://doi.org/10.5281/zenodo.5772122.

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Ravishankar College, Bhanpur Chouraha, Bhopal-462 010, Madhya Pradesh, India Quality Control Head - Glenmark Pharmaceutical Ltd. <em>Manuscript received online 22 February 2012, revised 28 March 2012, accepted 30 March 2012</em> An ultra performance liquid chromatographic (UPLC) method was developed for simultaneous determination of antifungal ointment and active pharmaceutical ingredient (API) residues. A new, generic method is presented, with which it is possible to verify the cleaning process of antifungal drug producing equipment line used for the production of various pharmaceuticals. The

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Neue, Uwe D., Christopher S. Messina, and Diane M. Diehl. "UPLC-Methoden entwickeln und optimieren." Nachrichten aus der Chemie 55, no. 11 (2007): 1120–21. http://dx.doi.org/10.1002/nadc.200753786.

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Bertolín, J. R., M. Joy, and M. Blanco. "Malondialdehyde determination in raw and processed meat products by UPLC-DAD and UPLC-FLD." Food Chemistry 298 (November 2019): 125009. http://dx.doi.org/10.1016/j.foodchem.2019.125009.

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Chen, Chien-Lun, Yi-Ting Chen, Wen-Yu Liao, Yu-Sun Chang, Jau-Song Yu, and Bao-Rong Juo. "Urinary Metabolomic Analysis of Prostate Cancer by UPLC-FTMS and UPLC-Ion Trap MSn." Diagnostics 13, no. 13 (2023): 2270. http://dx.doi.org/10.3390/diagnostics13132270.

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Accumulative evidence suggests metabolic disorders correlate with prostate cancer. Metabolic profiling of urine allows the measurement of numerous metabolites simultaneously. This study set up a metabolomic platform consisting of UPLC-FTMS and UPLC-ion trap MSn for urine metabolome analysis. The platform improved retention time, mass accuracy, and signal stability. Additionally, the product ion spectrum obtained from ion trap MSn facilitated structure elucidation of candidate metabolites, especially when authentic standards were not available. Urine samples from six hernia patients and six BPH

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Hebha, Amreen*. "HARNESSING SUB-2 μM TECHNOLOGY: THE TRANSFORMATIVE ROLE OF UPLC IN PHARMACEUTICAL ANALYSIS". World Journal of Advance Pharmaceutical Sciences 2, № 1 (2025): 68–73. https://doi.org/10.5281/zenodo.15389535.

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Ultra-Performance Liquid Chromatography (UPLC) has emerged as apivotal advancement in pharmaceutical analysis, leveraging sub-2 μmparticle technology to achieve unprecedented speed, resolution, andsensitivity. While traditional High-Performance Liquid Chromatography(HPLC) remains a cornerstone, UPLC’s superior efficiency—governed bythe van Deemter equation—enables faster separations with enhanced peakcapacity. However, its adoption necessitates careful consideration ofoperational challenges, including increased backpressure, method scalability,and economic trade-offs. This

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Xue, Ying, Wei Jin, Xian-Shun Xu, et al. "Quality Evaluation of Tricholoma matsutake based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS." Molecules 24, no. 1 (2018): 34. http://dx.doi.org/10.3390/molecules24010034.

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So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanos

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Bharani Pandilla, Chitra K, Nalini C N, Ashok P, and Vadivelan R. "Method development and validation of Ifetroban by RP-UPLC." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (2020): 3158–63. http://dx.doi.org/10.26452/ijrps.v11i3.2429.

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The purpose of this work is to develop and validate reverse phase Ultra performance liquid chromatography (UPLC) method for the rapid and precise determination of ifetroban sodium in its pure form and in formulations. A simple, specific, accurate, precise isocratic UPLC method for analysis of Ifetroban sodium was developed and validated using a Phenomenex C18 column (50 mm x 3.0 mm, 3µ) as the stationary phase, in conjunction using Triethyl amine buffer: methanol in the proportion of 25:75 with a flow rate of 1.0 mL/min, run time is 3 min and UV detector is used at 235 nm wavelength. The devel

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Harp, Bhakti Petigara, Nebebech Belai, and Julie N. Barrows. "Ultra-Performance Liquid Chromatographic Determination of Manufacturing Intermediates and Subsidiary Colors in D&C Red No. 34 and Its Lakes." Journal of AOAC INTERNATIONAL 94, no. 5 (2011): 1548–54. http://dx.doi.org/10.1093/jaoac/94.5.1548.

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Abstract An ultra-performance liquid chromatography (UPLC) method was developed to determine the manufacturing intermediates and subsidiary colors in the monosulfo monoazo color additive D&C Red No. 34 and its lakes. This method is currently used for batch certification of the color additives by the U.S. Food and Drug Administration to ensure that each lot meets published specifications for coloring drugs and cosmetics. The new UPLC method has replaced an HPLC method for determining the intermediates and a TLC method for determining the subsidiary colors. The intermediates are 2-amino-1-na

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Amin, Elham, Yan-Hong Wang, Bharathi Avula, et al. "Simultaneous Determination of Saponins and a Flavonoid from Aerial Parts of Zygophyllum coccineum L." Journal of AOAC INTERNATIONAL 95, no. 3 (2012): 757–62. http://dx.doi.org/10.5740/jaoacint.11-216.

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Abstract Triterpenoid saponins are a class of glycosides with a wide range of bioactivities, which make them interesting research candidates. Zygophyllum coccineum is an Egyptian desert plant rich in triterpenoid saponins. Reviewing the relevant literature, no data concerning the HPLC or ultra-performance LC (UPLC) analysis of Zygophyllum content were found. This paper presents two methods, HPLC-UV and UPLC-UV-evaporative light scattering detector (ELSD)/MS, for the simultaneous determination of 10 compounds in the alcohol extract of Z. coccineum. The HPLC method uses a C18 column and water–ac

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Gao, Weixi, and Yan Zhuang. "Rapid Detection of Chloramphenicol Residues in Fermented Foods Based on UPLC-DAD." Tobacco Regulatory Science 7, no. 6 (2021): 6303–16. http://dx.doi.org/10.18001/trs.7.6.105.

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in the detection of chloramphenicol residues in fermented food, there are often problems of slow detection speed. Using UPLC-DAD method, a rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method is designed. According to the characteristics of chloramphenicol, set up the detection reagent, select the detection equipment, and form the detection laboratory. It is usingUPLC-DAD method to design the test paper, using the set test reagent to deal with the sample to be tested, according to the design results of the test process, combining the reagent with the sa

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Attwa, Mohamed W., Haitham AlRabiah, Aishah M. Alsibaee, Ali S. Abdelhameed, and Adnan A. Kadi. "An UPLC–ESI–MS/MS Bioanalytical Methodology for the Quantification of Gilteritinib in Human Liver Microsomes: Application to In Vitro and In Silico Metabolic Stability Estimation." Separations 10, no. 5 (2023): 278. http://dx.doi.org/10.3390/separations10050278.

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Gilteritinib (Xospata®) is a tyrosine kinase inhibitor (TKI) that works by inhibiting numerous receptor tyrosine kinases, involving AXL and FMS-like tyrosine kinase 3 (FLT3). Gilteritinib (GTB) was approved (28 November 2018) by the USFDA for the treatment of refractory or relapsed (R/R) acute myeloid leukemia (AML) with a FLT3 mutation. In the current study, a fast, highly sensitive, and specific ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) analytical methodology was created for GTB determination in human liver microsomes (HLMs) utilizing an electrospray ioniz

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Lee, Loong Chuen, Nadirah Abd Hamid, Nur Ain Najihah Mohd Rosdi, and Hukil Sino. "Evaluation of Column-Wise Manipulations on Ultra-Performance Liquid Chromatography (UPLC) Data for Forensic Soil Discrimination." EDUCATUM Journal of Science, Mathematics and Technology 9, no. 1 (2022): 99–107. http://dx.doi.org/10.37134/ejsmt.vol9.1.10.2022.

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Soil is one of the most encountered physical evidence and can be useful in tracing the location of the crime scene. The discrimination of soils is fundamental to provide a link between a suspect and a crime scene. However, discrimination study of soils could be difficult due to interferences in the chemical fingerprint of soils obtained via a chemical instrumental technique. In this study, performances of four column-wise manipulations (CWMs) on ultra-performance liquid chromatography (UPLC) data of soils were evaluated. Both univariate and multivariate exploratory tools have been employed to

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Yoo, SeongEui, WooSoo Jeong, Soo-Hwan Yeo, and So-Young Kim. "Method validation for quantitative analyzing aflatoxin productivity in Aspergillus sp. isolated from soybean paste." Korean Journal of Food Preservation 30, no. 1 (2023): 28–41. http://dx.doi.org/10.11002/kjfp.2023.30.1.28.

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Non-aflatoxigenic Aspergillus oryzae and aflatoxigenic A. flavus cannot be clearly identified by partial sequencing of the internal transcribed spacer (ITS) and 18S ribosomal ribonucleic acid (18S rRNA) regions. This study aimed to compare the accuracy among three aflatoxin detection methods using ultra-performance liquid chromatography (UPLC), high-performance liquid chromatography (HPLC), and an enzyme-linked immunosorbent assay (ELISA) kit and to select the non-aflatoxigenic Aspergillus sp. isolated from soybean paste. All analytical methods were suitable according to the international stan

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Manda, Swapna* Rongali Indu Dr. P.V. Madhavi Latha B. Rama Madhuri. "A Comprehensive Review on UHPLC and UPLC: Advancements, Comparison, and Applications." International Journal of Scientific Research and Technology 1, no. 11 (2024): 87–91. https://doi.org/10.5281/zenodo.14173528.

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Ultra-High-Performance Liquid Chromatography (UHPLC) and Ultra Performance Liquid Chromatography (UPLC) are two of the most advanced liquid chromatographic techniques, offering improved separation, sensitivity, and speed over traditional High-Performance Liquid Chromatography (HPLC). While both technologies share similar principles, they differ in system configurations, column technologies, and applications. UHPLC generally refers to any liquid chromatography system capable of using sub-2 µm particles, while UPLC is a proprietary technology developed by Waters Corporation that optimizes

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Chen, Zhuo, Yun W. Alelyunas, Mark D. Wrona, Jonathan R. Kehler, Matthew E. Szapacs, and Christopher A. Evans. "Microflow UPLC and high-resolution MS as a sensitive and robust platform for quantitation of intact peptide hormones." Bioanalysis 11, no. 13 (2019): 1275–89. http://dx.doi.org/10.4155/bio-2019-0081.

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Aim: Recent advances in microflow ultra performance liquid chromatography (UPLC) systems offer higher sensitivity with robustness to meet the routine bioanalytical demands. Modern high-resolution mass spectrometers (HRMS) enable the development of highly selective methods with broad dynamic range. Results: The quantitative performances of tandem quadrupole MS and HRMS were comprehensively compared using seven intact peptide hormones up to 9.4 kDa. Results show comparable performance between two platforms in sensitivity, accuracy and linearity. For some peptides, HRMS provided lower background

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Wu, Di, Han Wang, Jing Tan, et al. "Pharmacokinetic and Metabolism Studies of Curculigoside C by UPLC-MS/MS and UPLC-QTOF-MS." Molecules 24, no. 1 (2018): 21. http://dx.doi.org/10.3390/molecules24010021.

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Pharmacokinetic and metabolism studies were carried out on curculigoside C (CC), a natural product with good antioxidant and neuroprotective effects, with the purpose of investigating the effects of the hydroxyl group at C-3′ in curculigoside. A rapid and sensitive method with UPLC-MS was developed and fully validated for the first time in the pharmacokinetic analysis for quantification of CC in rat plasma. The assay was linear (R2 > 0.9984) over the concentration range of 1–2500 ng/mL, with the lower limit of quantification (LLOQ) being 1 ng/mL. The intra-day and inter-day precision (expre

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Zhang, Yuchi, Chunming Liu, Zhengkun Zhang, Yanjuan Qi, Guimei Wu, and Sainan Li. "Fast differentiation of Apocynum venetum with related species by UPLC/MS and UPLC/SPE/NMR." Medicinal Chemistry Research 21, no. 7 (2011): 1077–83. http://dx.doi.org/10.1007/s00044-011-9624-2.

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Basharat, Rabia, Vijay Kotra, Lean Yen Loong, et al. "A Mini-review on Ultra Performance Liquid Chromatography." Oriental Journal Of Chemistry 37, no. 4 (2021): 847–57. http://dx.doi.org/10.13005/ojc/370411.

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Chromatography is a widely used analytical tool for separating a mixture of compounds into individual component. High performance liquid chromatography (HPLC) is one of the most important methods used for the separation, identification and quantification of a compounds present in a mixture. It meets many criteria of analysis but its main drawbacks are it is relatively time consuming to run a chromatogram and consumes high amount of solvent compared to other analytical methods. There is a need to develop a method which can overcome these drawbacks of HPLC. Ultra performance liquid chromatograph

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Kanchan, Shinde* Neha Gaikwad Varsha Pangale Charushila Bhangale. "A Review On Development And Validation Of Ultra Performance Liquid Chromatography For Estimation Of Drug In Pharmaceutical Dosage Form By Quality By Design (Qbd) Approach." Int. J. in Pharm. Sci. 1, no. 7 (2023): 123–38. https://doi.org/10.5281/zenodo.8112767.

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The pharmaceutical business, system optimization, stationary phase vs mobile phase, detector design, data processing, and data integrity control have all seen notable technical advancements in recent years. The individual accomplishments in each subject have led to a step-function increase in technique and performance when technology and quality are combined. A new method of using liquid chromatography for sample detection is provided by ultra-performance liquid chromatography (UPLC). UPLC primarily improves the resolution, speed, and sensitivity of liquid chromatography. UPLC approach uses Qu

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Yu, Mengyang, Aijing Liu, Shupeng Liu, et al. "Development and validation of a UPLC–MS/MS method for determination of SYHA1807 in a first-in-human study." Bioanalysis 15, no. 24 (2023): 1489–501. http://dx.doi.org/10.4155/bio-2023-0143.

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Background: SYHA1807 is a novel lysine specific demethylase 1 inhibitor being developed for the treatment of small-cell lung cancer. Aim: This study aimed to establish a ultra-performance liquid chromatography-mass spectrometry (UPLC–MS)/MS method for measuring SYHA1807 in human plasma, supporting its application in a first-in-human study. Methods: SYHA1807 was separated on an ACQUITY UPLC BEH® C18 Column (2.1 × 50 mm, 1.7 μm) after protein precipitation of plasma samples. Mass spectrometry analysis was performed with a Xevo TQS triple quadrupole mass spectrometer utilizing a positive electron

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