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Journal articles on the topic 'Uranyl acetate'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Barabas, L., C. Gorguinpour, A. F. Leung, and J. McCoy. "Spectroscopic Measurements of Temperature with Uranyl Compounds." Applied Spectroscopy 55, no. 10 (2001): 1382–84. http://dx.doi.org/10.1366/0003702011953513.

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A new class of fluorescent probes for measuring temperature is described. The intensities of the strong fluorescence bands of uranyl acetate, uranyl nitrate hexahydrate, and uranyl orthophosphate were recorded when the uranyl compound was illuminated with an argon-ion laser operating at 488 nm. The intensity ratio of two fluorescence bands of uranyl acetate at room temperature were determined for five freshly prepared samples and shown to be independent of the intensity of the laser excitation and optical alignment. The intensities of the fluorescence bands lying between 500 and 570 nm were me

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2

R.C., Caughey, and Kalyan-Raman U.P. "Uranyl Acetate En Bloc Staining to Complement Diagnostic Electron Microscopic Evaluation of Muscle Biopsies." Proceedings, annual meeting, Electron Microscopy Society of America 43 (August 1985): 462–63. http://dx.doi.org/10.1017/s0424820100119132.

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Uranyl acetate en bloc staining is known to improve overall contrast and membrane preservation and removes glycogen from tissues. This staining procedure complements diagnostic electron microscopic evaluation by enhancing ultrastructural details. The ultrastructural structures enhanced are basement and plasma membranes, mitochondrial membrane and cristae, sarcoplasmic reticulum and T-tubule system. We wanted to study the usefulness of this technique over the conventional method in the study of muscle biopsy. Ultrastructurally, thirty muscle biopsies submitted to our Electron Microscopic Lab we

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3

Kitahara, Keisuke, Chiya Numako, Yasuko Terada, Kiyohumi Nitta, Yoshiya Shimada, and Shino Homma-Takeda. "Uranium XAFS analysis of kidney from rats exposed to uranium." Journal of Synchrotron Radiation 24, no. 2 (2017): 456–62. http://dx.doi.org/10.1107/s1600577517001850.

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The kidney is the critical target of uranium exposure because uranium accumulates in the proximal tubules and causes tubular damage, but the chemical nature of uranium in kidney, such as its chemical status in the toxic target site, is poorly understood. Micro-X-ray absorption fine-structure (µXAFS) analysis was used to examine renal thin sections of rats exposed to uranyl acetate. The ULIII-edge X-ray absorption near-edge structure spectra of bulk renal specimens obtained at various toxicological phases were similar to that of uranyl acetate: their edge position did not shift compared with th

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4

Grigor'ev, M. S., M. Yu Antipin, and N. N. Krot. "Behavior of Anhydrous Uranyl Acetate at Heating in CH3CN. Crystal Structures of New Uranyl Acetates." Radiochemistry 46, no. 3 (2004): 224–31. http://dx.doi.org/10.1023/b:rach.0000031676.61250.09.

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5

Harris, J. Robin, Max Gerber, Wolfgang Gebauer, Wolfgang Wernicke, and Jürgen Markl. "Negative Stains Containing Trehalose: Application to Tubular and Filamentous Structures." Microscopy and Microanalysis 2, no. 1 (1996): 43–52. http://dx.doi.org/10.1017/s1431927696210438.

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Several examples are presented that show the successful application of uranyl acetate and ammonium molybdate negative staining in the presence of trehalose for TEM studies of filamentous and tubular structures. The principal benefit to be gained from the inclusion of trehalose stems from the considerably reduced flattening of the large tubular structures and the greater orientational freedom of single molecules due to an increased depth of the negative stain in the presence of trehalose. Trehalose is likely to provide considerable protection to protein molecules and their assemblies during the

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6

Lal, Ram A., Manindra N. Singh, and Sujit Das. "Complexes of Uranyl Nitrate, Uranyl Acetate, Uranyl Thiocyanate and Uranyl Chloride with Benzoyl, Salicyloyl and Isonicotinoyl Hydrazines." Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry 16, no. 4 (1986): 513–25. http://dx.doi.org/10.1080/00945718608055925.

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7

Schneider, Jan Philipp, and Matthias Ochs. "Alterations of mouse lung tissue dimensions during processing for morphometry: A comparison of methods." American Journal of Physiology-Lung Cellular and Molecular Physiology 306, no. 4 (2014): L341—L350. http://dx.doi.org/10.1152/ajplung.00329.2013.

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Preservation of original tissue dimensions is an essential prerequisite for morphometric studies. Shrinkage occurring during tissue processing for histology may severely influence the appearance of structures seen under the microscope and stereological calculations. Therefore, shrinkage has to be avoided so that estimates obtained by application of unbiased stereology are indeed unbiased. The present study investigates the alterations of tissue dimensions of mouse lung samples during processing for histology. Different fixatives as well as embedding protocols are considered. Mouse lungs were f

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8

Sano, Koji, Yoshihide Fujigaki, Takehiko Miyaji, et al. "Role of apoptosis in uranyl acetate-induced acute renal failure and acquired resistance to uranyl acetate." Kidney International 57, no. 4 (2000): 1560–70. http://dx.doi.org/10.1046/j.1523-1755.2000.00777.x.

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9

Mrazova, Katerina, Jaromir Bacovsky, Zuzana Sedrlova, et al. "UranyLess Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells." Microorganisms 11, no. 4 (2023): 888. http://dx.doi.org/10.3390/microorganisms11040888.

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Sample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on

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10

Soeldner, Al. "Uranium Is The Issue." Microscopy Today 6, no. 9 (1998): 24–25. http://dx.doi.org/10.1017/s1551929500069522.

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Uranium compounds, especially uranyl acetate, have been widely and routinely used as transmission electron microscopy centrist stains for biological materials since 1958. Those of us who do TEM of biologicals use small quantities of uranyl acetate, nitrate, formate, sulfate and perhaps other uranium compounds almost daily and therefore keep inventories of these salts and their solutions.In the 1980's growing concerns about medical and research wastes entering regional dump sites prompted state radiation officials in Oregon to begin tightening the regulations for monitoring and controlling all

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11

PRASAD, Shiva, and Jaqueline Viana BARROS. "Electrometric studies on uranyl molybdates as a function of pH." Eclética Química 23 (1998): 59–70. http://dx.doi.org/10.1590/s0100-46701998000100005.

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The formation and composition of uranyl molybdates obtained by the interaction of uranyl acetate and sodium molybdate at specific pH levels 7.6, 5.5 and 4.1 have been studied by employing electrometric techniques involving pH and conductometric titrations. The results provide cogent evidence for the formation of three uranyl molybdates having the molecular formulae UO2O.MoO3, 3UO2O.7MoO3 and 2UO2O.8MoO3 in the vicinity of pH 5.7, 4.6 and 3.8, respectively. Analytical investigations of the compounds have also been carried out which substantiate the results of electrometric study.

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12

Prasad, Shiva, and Jaqueline Viana Barros. "Electrometric studies on uranyl molybdates as a function of pH." Ecletica Quimica 23, no. 1 (1998): 59–70. http://dx.doi.org/10.26850/1678-4618eqj.v23.1.1998.p59-70.

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The formation and composition of uranyl molybdates obtained by the interaction of uranyl acetate and sodium molybdate at specific pH levels 7.6, 5.5 and 4.1 have been studied by employing electrometric techniques involving pH and conductometric titrations. The results provide cogent evidence for the formation of three uranyl molybdates having the molecular formulae UO2O.MoO3, 3UO2O.7MoO3 and 2UO2O.8MoO3 in the vicinity of pH 5.7, 4.6 and 3.8, respectively. Analytical investigations of the compounds have also been carried out which substantiate the results of electrometric study.

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13

Takase, Hiroshi, and Makoto Sugiura-Nakazato. "PB-05 Recycling of Uranyl acetate solution." Microscopy 68, Supplement_1 (2019): i48. http://dx.doi.org/10.1093/jmicro/dfz082.

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14

Monshi, M. A. S., N. M. Abd El-Salam та R. M. Mahfouz. "Isothermal decomposition of γ-irradiated uranyl acetate". Thermochimica Acta 322, № 1 (1998): 33–37. http://dx.doi.org/10.1016/s0040-6031(98)00488-2.

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15

Tandler, Bernard. "Improved uranyl acetate staining for electron microscopy." Journal of Electron Microscopy Technique 16, no. 1 (1990): 81–82. http://dx.doi.org/10.1002/jemt.1060160110.

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16

Shepard, Nora, and Nelson Mitchell. "Simultaneous demonstration of matrix lipid and proteoglycan in rat growth plate through selective preservation: A new technique." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 404–5. http://dx.doi.org/10.1017/s042482010010408x.

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The presence of lipid in cartilage has been determined through biochemical analysis and associated with mineralization (1,2,3), as is proteoglycan (4,5). However, unlike proteoglycan, an ultrastructural technique does not exist for cartilage lipid stabilization.Nile blue sulphate, a dye used in fluorescence microscopy to study lipids, when added to the initial glutaraldehyde fixation and followed by potassium ferrocyanide reduced osmium and en bloc, uranyl acetate, retained lipids in the cartilage matrix. In addition to the lipid stabilization, matrix proteoglycan was also rendered insoluable,

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17

Kawkab S.Najim and Hussain I. Hussain. "Histological changes study of the White rat heart muscle treated with Uranyl acetate." Tikrit Journal of Pure Science 22, no. 4 (2023): 44–49. http://dx.doi.org/10.25130/tjps.v22i4.732.

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The present investigation aimed to study the histological changes of heart muscle treated with Uranyl acetate for one & two months. The results showed that there was histological changes in the heart muscles , these changes were more sever in animal group treated for two months. Since severe fibrosis was seen with congestion of blood vessel as wear of an increment of fatty degeneration compared with group one which was treated for one month and characterized with the presence of normal not effected region . The present study we may concluded that, Uranyl acetate induced severe negati

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18

LIMA, Francisco José Santos, Luiz Henrique Medeiros da COSTA, and Ademir Oliveira da SILVA. "SPECTROCHEMICAL STUDIES OF THE ION UO22+ COORDINATED IN THE URANYL ACETATE UO2(H3CCOO)2.2H2O." Periódico Tchê Química 12, no. 24 (2014): 33–46. http://dx.doi.org/10.52571/ptq.v11.n22.2014.33_periodico_22_pgs_33_46.pdf.

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The cation complex uranyl UO22+, is an molecular ion very stable. It possesses a linear geometry O=U=O, with connections U-O short of the order of 1,75 Å. Studies returned for to the fictionalization of this ion, for the insert of appropriate ligands it has been enough discussed in the literature. In this work we studied the transitions in the area of the uv-visible of the ion uranyl coordinated to the against-cation acetate, derived chemist of the acetic acid, in the compound UO2(H3CCOO)2.2H2O, known as uranyl acetate. For that your transitions were observed, calculated and compared by force

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19

Trapeznikova, Е. G., V. В. Popov, A. S. Radilov, and V. V. Shilov. "DOSE-DEPENDENT CHARACTER OF DISTURBANCE OF HEMATOPOIETIC AND IMMUNE SYSTEMS FUNCTION, PRODUCTION OF SOME HORMONES IN EXPERIMENTAL URANIUM ACETATE DIHYDRATE EXPOSURE." Toxicological Review 1, no. 1 (2021): 14–19. http://dx.doi.org/10.36946/0869-7922-2021-1-14-19.

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The paper presents the results of an experimental study of the dose-dependent nature of functional changes in the body systems under chronic administration of uranyl acetate dihydrate in doses of 0.5 and 5.0 mg/kg per element for 18 weeks. The study was performed on 45 male outbred rats. It has been shown that uranyl acetate dihydrate in a dose of 0.5 mg/kg had no significant effect on hematological parameters. At the same time, activation of bactericidal activity of neutrophils, a decrease in the immunoregulatory index, and an increase in the blood concentration of tumor necrosis factor (TNF-

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20

Stosman, К. I., К. V. Sivak, Т. A. Rassokha, and Т. N. Savateeva-Lubimova. "DOSE-DEPENDENT CHARACTER OF DISTURBANCE OF HEMATOPOIETIC AND IMMUNE SYSTEMS FUNCTION, PRODUCTION OF SOME HORMONES IN EXPERIMENTAL URANIUM ACETATE DIHYDRATE EXPOSURE." Toxicological Review 1, no. 1 (2021): 20–26. http://dx.doi.org/10.36946/0869-7922-2021-1-20-26.

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The paper presents the results of an experimental study of the dose-dependent nature of functional changes in the body systems under chronic administration of uranyl acetate dihydrate in doses of 0.5 and 5.0 mg/kg per element for 18 weeks. The study was performed on 45 male outbred rats. It has been shown that uranyl acetate dihydrate in a dose of 0.5 mg/kg had no significant effect on hematological parameters. At the same time, activation of bactericidal activity of neutrophils, a decrease in the immunoregulatory index, and an increase in the blood concentration of tumor necrosis factor (TNF-

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21

Tian, Jing, Chunyan Li, Shaopu Liu, et al. "A rapid and highly sensitive fluorimetric method for the determination of meloxicam using uranyl acetate." Anal. Methods 6, no. 14 (2014): 5221–26. http://dx.doi.org/10.1039/c4ay00809j.

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22

Chowdhury, Sanjib, Leonor Maria, Adelaide Cruz, et al. "Uranium Carbide Fibers with Nano-Grains as Starting Materials for ISOL Targets." Nanomaterials 10, no. 12 (2020): 2458. http://dx.doi.org/10.3390/nano10122458.

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This paper presents an experimental study about the preparation, by electrospinning, of uranium carbide fibers with nanometric grain size. Viscous solutions of cellulose acetate and uranyl salts (acetate, acetylacetonate, and formate) on acetic acid and 2,4-pentanedione, adjusted to three different polymer concentrations, 10, 12.5, and 15 weight %, were used for electrospinning. Good quality precursor fibers were obtained from solutions with a 15% cellulose acetate concentration, the best ones being produced from the uranyl acetate solution. As-spun precursor fibers were then decomposed by slo

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23

Горелик, В. С., С. О. Нечипуренко та А. А. Лобойко. "Зондовая спектроскопия комбинационного рассеяния света поликристаллов ураниловых соединений". Журнал технической физики 127, № 10 (2019): 536. http://dx.doi.org/10.21883/os.2019.10.48353.164-19.

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AbstractRegularities in the Raman spectra of acetate uranyl compounds CsUO_2(CH_3COO)_3, CsUO_2(CD_3COO)_3, NaUO_2(CH_3COO)_3, NaUO_2(CD_3COO)_3, UO_2(CH_3COO)_2, and RbUO_2(CH_3COO)_3 have been analyzed. The Raman spectra are excited by 785-nm laser radiation and recorded using a miniature fiber-optic spectrometer with a multielement detector. It is established that the spectra of all these compounds contain a strong Raman satellite with a frequency in the range of 850–860 cm^–1. This satellite corresponds to symmetric stretching vibrations (type A _1) of the uranyl group. The obtained result

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24

Njiki Noufele, Christelle, Dennis Schulze, Maximilian Roca Jungfer, Adelheid Hagenbach, and Ulrich Abram. "Bimetallic Uranium Complexes with 2,6-Dipicolinoylbis(N,N-Dialkylthioureas)." Molecules 29, no. 21 (2024): 5001. http://dx.doi.org/10.3390/molecules29215001.

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2,6-Dipicolinoylbis(N,N-dialkylthioureas), H2LR, readily react with uranyl salts under formation of monomeric or dimeric complexes of the compositions [UO2(LR)(solv)] (solv = donor solvents such as H2O, MeOH or DMF) or [{UO2(LR)(µ-OMe)}2]2− (1). In such complexes, the uranyl ions are exclusively coordinated by the “hard” O,N,O or N,N,N donor atom sets of the central ligand unit and the lateral sulfur donor atoms do not participate in the coordination. Different conformations have been found for the dimeric anions. The bridging methanolato ligands and the four uncoordinated sulfur atoms can ado

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25

Gorelik, V. S., A. A. Anik’ev, V. M. Korshunov, and Yu P. Voinov. "Probe Raman spectroscopy of sodium uranyl-acetate microcrystals." Optics and Spectroscopy 123, no. 2 (2017): 255–57. http://dx.doi.org/10.1134/s0030400x17080070.

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26

Perez, Evan, Cassandra Hanley, Stephen Koehler, Jordan Pestok, Nevo Polonsky, and Michael Van Stipdonk. "Gas Phase Reactions of Ions Derived from Anionic Uranyl Formate and Uranyl Acetate Complexes." Journal of The American Society for Mass Spectrometry 27, no. 12 (2016): 1989–98. http://dx.doi.org/10.1007/s13361-016-1481-2.

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27

Hollóczki, Oldamur. "Uranyl(VI) Complexes in and from Imidazolium Acetate Ionic Liquids: Carbenes versus Acetates?" Inorganic Chemistry 53, no. 2 (2013): 835–46. http://dx.doi.org/10.1021/ic402921b.

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28

Shashi, B. Kalla, Kaushal Geetanjli, and C. Verma B. "Spectrophotometric method for the determination of isoniazid." Journal of Indian Chemical Society Vol. 83, Jan 2006 (2006): 83–84. https://doi.org/10.5281/zenodo.5816480.

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Department of Chemistry, Himachal Pradesh University, Summer Hill. Shimla-171 005. Himachal Pradesh. India <em>E-mail :</em> shashibalakalia@rediffmail.com <em>Manuscript received 17 May 2004. revised 24 June 2005. accepted 13 August 2005</em> The observation that carbon disulphide transforms isoniazid in methanol medium into isonicotinyldithiocarbazic acid and the latter reacts with uranyl acetate in the same medium rapidly and quantitatively to form soluble bright yellow uranyl isonicotinyldithiocarbazate complex. has been made the basis of a simple, rapid and sensitive spectrophotometric me

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29

Caughey, R. C., and U. P. Kalyan-Raman. "Nonspecific structures seen in diagnostic muscle biopsies." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 846–47. http://dx.doi.org/10.1017/s0424820100128511.

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In a period of two years we have analyzed 50 muscle biopsies using the transmission electron microscope. Six nonspecific structures consisting of filamentous bodies, tubular aggregates, paracrystalline mitochondrial inclusions, honeycomb arrays, concentric laminated bodies, and finger print profiles were observed in 47 of 50 cases. In order to know the significance of these structures in muscle biopsies, we correlated their occurrence with their clinical history, histological findings, and histochemistry.The biopsies were initially fixed in 2.5% glutaraldehyde (pH. 7.5, 500 mOsm), then randoml

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30

Gorelik, V. S., A. A. Anik’ev, V. M. Korshunov, Yu P. Voinov, and A. A. Loboyko. "Raman scattering and photoluminescence in sodium uranyl acetate polycrystals." Physics of Wave Phenomena 25, no. 4 (2017): 272–75. http://dx.doi.org/10.3103/s1541308x17040057.

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31

Gorelik, V. S., V. M. Korshunov, and Yu P. Voinov. "Resonance excitation of photoluminescence in sodium uranyl acetate crystals." Optics and Spectroscopy 121, no. 6 (2016): 819–22. http://dx.doi.org/10.1134/s0030400x16120122.

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32

Gorelik, V. S., A. A. Loboiko, and S. O. Nechipurenko. "Resonance Excitation of Photoluminescence in Crystalline Uranyl Acetate Dihydrate." Optics and Spectroscopy 124, no. 2 (2018): 221–26. http://dx.doi.org/10.1134/s0030400x1802008x.

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33

Ozmen, Murat, and Muhittin Yurekli. "Subacute toxicity of uranyl acetate in Swiss-Albino mice." Environmental Toxicology and Pharmacology 6, no. 2 (1998): 111–15. http://dx.doi.org/10.1016/s1382-6689(98)00025-8.

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34

Murry, Maisha M., Stephen P. LaMont, Samuel E. Glover, and Henry B. Spitz. "In vitro dissolution of uranyl acetate using different methods." Journal of Radioanalytical and Nuclear Chemistry 296, no. 2 (2012): 909–13. http://dx.doi.org/10.1007/s10967-012-2221-0.

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35

Donova, Ilinka, Slobotka Aleksovska, and Viktor Stefov. "Synthesis, characterization and thermal decomposition of pyridinium uranyl acetate." Thermochimica Acta 348, no. 1-2 (2000): 169–74. http://dx.doi.org/10.1016/s0040-6031(00)00362-2.

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36

MacCordick, H. J. "Complexes of uranyl formate and acetate with mycobactin S." Journal of Radioanalytical and Nuclear Chemistry Letters 128, no. 3 (1988): 249. http://dx.doi.org/10.1007/bf02166656.

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37

Czaban, B. Barbara, and Arthur Forer. "Visualization of ultraviolet microbeam irradiation sites with uranyl acetate." Journal of Microscopy 164, no. 1 (1991): 61–65. http://dx.doi.org/10.1111/j.1365-2818.1991.tb03192.x.

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38

MacCordick, H. J. "Complexes of uranyl formate and acetate with mycobactin S." Journal of Radioanalytical and Nuclear Chemistry Letters 126, no. 2 (1988): 173–81. http://dx.doi.org/10.1007/bf02162436.

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39

Fassel, Theresa A., and Marion L. Greaser. "Uranyl acetate as a primary fixative for skeletal muscle." Microscopy Research and Technique 37, no. 5-6 (1997): 600–601. http://dx.doi.org/10.1002/(sici)1097-0029(19970601/15)37:5/6<600::aid-jemt21>3.0.co;2-s.

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40

Fan, Jinda, John J. Bozzola, and Yong Gao. "Encapsulation of Uranyl Acetate Molecules Using Hollow Polymer Templates." Journal of Colloid and Interface Science 254, no. 1 (2002): 108–12. http://dx.doi.org/10.1006/jcis.2002.8581.

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41

Cross, R. H. M., A. N. Hodgson, and R. T. F. Bernard. "Uranyl acetate staining under different conditions of preparation, storage and use." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 726–27. http://dx.doi.org/10.1017/s0424820100161199.

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Uranyl acetate is routinely used in the staining of thin sections of biological tissue for transmission electron microscopy. Although many methods for its preparation and use have been described, there is seldom reference to the reasons for variations in concentration, solvent, storage time and staining time. Likewise, possible variations in the effects of staining under different conditions are largely ignored. In order to gain clarity on this issue an attempt has been made to test three variables (solvent, storage time and use in light or dark) under controlled experimental conditions.The ti

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42

Caughey, R. C., and C. E. Kelly. "Ultrastructural observations in two cases of acute pyelonephritis." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 364–65. http://dx.doi.org/10.1017/s0424820100103887.

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In two histologically diagnosed cases of acute pyelonephritis, several ultrastructural features were observed. The transmission electron microscope (TEM) highlights the bacterial adhesion, inflammatory process, phagocytosis and lysosomal release characteristic of pyelonephritis.The renal biopsies from both patients were fixed in 2.5% glutaraldehyde, rinsed with Millonig&#x0027;s buffer, and post fixed with 1% osmium tetroxide. They were then en bloc stained with 0.5% uranyl acetate, rinsed with Walpole&#x0027;s buffer, dehydrated with a graded series of ethanols and embedded with Epon

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43

Segall, Iris, Olga L. Shaffer, Victoria L. Dimonie, and Mohamed S. El-Aasser. "Morphological study of structured latex particles by Transmission Electron Microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 882–83. http://dx.doi.org/10.1017/s0424820100150241.

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Transmission electron microscopy plays an important role in the study of the influence polymerization conditions have on the morphology of structured latex particles and thus in the understanding of the morphological effect of such particles on the structure-property relationships of polymeric end products.Structured latex particles are prepared by seeded emulsion polymerization, where the first stage is a polymerization of ”the core” poly(n-butyl acrylate) (PBA), followed by a second stage polymerization of ”the shell” poly(benzyl methacrylate/styrene) (P(BM/St)) at various ratios. The change

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44

Caughey, R. C., and C. E. Kelly. "Ultrastructural comparison of amyloid-like glyomerulopathy." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 216–17. http://dx.doi.org/10.1017/s0424820100158625.

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Without transmission electron microscopy cases of amyloidosis-like glomerulopathy may be erroneously considered to be amyloidosis, diabetic or membranous nephropathy. Mesangial widening and increased matrix, and capillary basement membrane thickening are features common to amyloid-like glomerulopathy, amyloidosis, and diabetic renal problems. Amyloid-like immunofluorescence may be positive for IgG, and C3 within the mesangium and capillary walls suggesting possible membranous nephropathy. Some cases appear to represent light chain nephropathy, which is known to result in crystalline-like ultra

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45

Rybicka, Krystyna. "Histogenesis of alveolar cell carcinoma." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 626–27. http://dx.doi.org/10.1017/s0424820100127566.

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Alveolar cell carcinoma (ACC) is a lung neoplasm characterized by the presence of lamellar bodies specific for normal type 2 alveolar cells. Tumor histogenesis is uncertain. The present study indicates that ACC originates from dedifferentiation of hyperplastic type 2 alveolar cells rather than migration of bronchial stem cells into alveoli as suggested earlier.An aliquot of human lung biopsy diagnosed as ACC was fixed in glutaraldehyde and osmium, treated with 1% aqueous uranyl acetate, and embedded in epoxy resin. Sections were stained for glycogen by periodic acid - thiosemicarbazide - silve

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Henry, Caroll E., T. L. Salaam, E. Steward-Clark, Joyce Craig, and Lennell Reynolds. "Characterization of Ustilago Hordei Fimbriae Using Scanning Transmission Electron Microscopy and Immunocytochemistry." Microscopy and Microanalysis 3, S2 (1997): 123–24. http://dx.doi.org/10.1017/s1431927600007509.

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Sporidia of Ustilago hordei produce surface fimbriae which are important in conjugation and pathogenicity. This work focuses on fimbrial origin and production using transmission electron microscopy (TEM) and immunocytochemistry.Wild type I4A sporidial cells cultured to log phase with rotary shaking in yeast extract glucose (YEG) growth for 48 h. at 21° C, were harvested by centrifugation at 8000 rpm, placed on formvar coated grids, negatively stained with 2% uranyl acetate and photographed in the JEOL 1200 STEM. Some cells were prepared for sectioning by fixation with gluteraldehyde and cacody

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McDonald, Kent L. "Rapid Embedding Methods into Epoxy and LR White Resins for Morphological and Immunological Analysis of Cryofixed Biological Specimens." Microscopy and Microanalysis 20, no. 1 (2013): 152–63. http://dx.doi.org/10.1017/s1431927613013846.

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AbstractA variety of specimens including bacteria, ciliates, choanoflagellates (Salpingoeca rosetta), zebrafish (Danio rerio) embryos, nematode worms (Caenorhabditis elegans), and leaves of white clover (Trifolium repens) plants were high pressure frozen, freeze-substituted, infiltrated with either Epon, Epon-Araldite, or LR White resins, and polymerized. Total processing time from freezing to blocks ready to section was about 6 h. For epoxy embedding the specimens were freeze-substituted in 1% osmium tetroxide plus 0.1% uranyl acetate in acetone. For embedding in LR White the freeze-substitut

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Goldsmith, C. S., H. G. Morrison, D. D. Auperin, S. G. Whitfield, and E. L. Palmer. "Vaccinia-lassa recombinant produces lassa-like inclusions." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 1036–37. http://dx.doi.org/10.1017/s0424820100157164.

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Lassa virus, a member of the Arenaviridae, is the etiologic agent of Lassa fever. The virus is endemic in widespread areas of West Africa, particularly Nigeria, Liberia, and Sierra Leone. Rough estimates indicate that there are upwards of 300,000 human infections annually, resulting in approximately 5000 fatalities. A safe and effective vaccine for Lassa fever is not yet available. Recent studies in our laboratories have focused on the development of recombinant vaccinia viruses that express the nucleoprotein and envelope glycoproteins of Lassa virus as potential vaccine candidates. Tissue cul

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Crooker, A. R., M. C. Myers, and W. G. Kraft. "Cellular and structural diversity of Campylobacter pylori." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 1058–59. http://dx.doi.org/10.1017/s0424820100157279.

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Campylobacter pylori is a recently recognized microaerophilic, gram-negative bacterium found in the upper gastrointestinal tract of humans. The role of this organism in the pathogenesis of gastritis and ulceration is currently under intense investigation. The rapidly growing literature on C. pylori describes the bacterium as spiral shaped; however, oval and coccal forms are occasionally noted. We report here on the cellular and structural diversity of this organism, including a new, pleomorphic form.Two Peruvian clinical isolates of C. pylori and the type strain NCTC 11638 were grown for up to

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Saving, K. L., and R. C. Caughey. "Electron Microscopy as an aid to diagnosis in pediatric hematology/oncology." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 1062–63. http://dx.doi.org/10.1017/s0424820100157292.

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This presentation is designed to demonstrate how scanning and transmission electron microscopy techniques can be utilized to confirm or support a variety of unusual pediatric hematologic/oncologic disorders. Patients with the following diagnoses will be presented: (1) hereditary pyropoikilocytosis, (2) familial erythrophagocytic lymphohistiocytosis, (3) acute megakaryoblastic leukemia, and (4) pseudo-von Willebrand’s disease.All transmission and scanning electron microscopy samples were fixed in 2.5% glutaraldehyde, rinsed in Millonig’s phosphate buffer, and post-fixed with 1% osmium tetroxide

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