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Journal articles on the topic 'Uranyl acetate'

Author: Grafiati

Published: 4 June 2021

Last updated: 17 February 2022

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1

Barabas, L., C. Gorguinpour, A. F. Leung, and J. McCoy. "Spectroscopic Measurements of Temperature with Uranyl Compounds." Applied Spectroscopy 55, no. 10 (October 2001): 1382–84. http://dx.doi.org/10.1366/0003702011953513.

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A new class of fluorescent probes for measuring temperature is described. The intensities of the strong fluorescence bands of uranyl acetate, uranyl nitrate hexahydrate, and uranyl orthophosphate were recorded when the uranyl compound was illuminated with an argon-ion laser operating at 488 nm. The intensity ratio of two fluorescence bands of uranyl acetate at room temperature were determined for five freshly prepared samples and shown to be independent of the intensity of the laser excitation and optical alignment. The intensities of the fluorescence bands lying between 500 and 570 nm were measured at various temperatures ranging from 77 to 295 K. The ratio of the intensities of two fluorescence bands was calculated and shown to vary linearly with temperature. A linear fit provided the slope. The largest positive slope of all the intensity ratios associated with uranyl acetate was 6.02 × 10−3 K−1, that associated with uranyl orthophosphate was 4.68 × 10−3 K−1, and that associated with uranyl nitrate hexahydrate was 1.81 × 10−3 K−1. The potential of uranyl compounds as a new group of temperature probes is discussed.

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2

Grigor'ev, M. S., M. Yu Antipin, and N. N. Krot. "Behavior of Anhydrous Uranyl Acetate at Heating in CH3CN. Crystal Structures of New Uranyl Acetates." Radiochemistry 46, no. 3 (May 2004): 224–31. http://dx.doi.org/10.1023/b:rach.0000031676.61250.09.

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3

R.C., Caughey, and Kalyan-Raman U.P. "Uranyl Acetate En Bloc Staining to Complement Diagnostic Electron Microscopic Evaluation of Muscle Biopsies." Proceedings, annual meeting, Electron Microscopy Society of America 43 (August 1985): 462–63. http://dx.doi.org/10.1017/s0424820100119132.

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Uranyl acetate en bloc staining is known to improve overall contrast and membrane preservation and removes glycogen from tissues. This staining procedure complements diagnostic electron microscopic evaluation by enhancing ultrastructural details. The ultrastructural structures enhanced are basement and plasma membranes, mitochondrial membrane and cristae, sarcoplasmic reticulum and T-tubule system. We wanted to study the usefulness of this technique over the conventional method in the study of muscle biopsy. Ultrastructurally, thirty muscle biopsies submitted to our Electron Microscopic Lab were routinely fixed in 2.5% glutaraldehyde and 1% 0S04, dehydrated in a graded series of ethanols, and embedded in Epon 812. Half of the blocks were processed and evaluated with post uranyl acetate/lead citrate staining. The other half were processed and evaluated by en bloc 0. 5% uranyl acetate/Walpole's buffer after 0S04 and before ethanol dehydration. The en bloc half were also stained with post uranyl acetate/lead citrate.

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4

Kitahara, Keisuke, Chiya Numako, Yasuko Terada, Kiyohumi Nitta, Yoshiya Shimada, and Shino Homma-Takeda. "Uranium XAFS analysis of kidney from rats exposed to uranium." Journal of Synchrotron Radiation 24, no. 2 (February 20, 2017): 456–62. http://dx.doi.org/10.1107/s1600577517001850.

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The kidney is the critical target of uranium exposure because uranium accumulates in the proximal tubules and causes tubular damage, but the chemical nature of uranium in kidney, such as its chemical status in the toxic target site, is poorly understood. Micro-X-ray absorption fine-structure (µXAFS) analysis was used to examine renal thin sections of rats exposed to uranyl acetate. The ULIII-edge X-ray absorption near-edge structure spectra of bulk renal specimens obtained at various toxicological phases were similar to that of uranyl acetate: their edge position did not shift compared with that of uranyl acetate (17.175 keV) although the peak widths for some kidney specimens were slightly narrowed. µXAFS measurements of spots of concentrated uranium in the micro-regions of the proximal tubules showed that the edge jump slightly shifted to lower energy. The results suggest that most uranium accumulated in kidney was uranium (VI) but a portion might have been biotransformed in rats exposed to uranyl acetate.

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5

Lal, Ram A., Manindra N. Singh, and Sujit Das. "Complexes of Uranyl Nitrate, Uranyl Acetate, Uranyl Thiocyanate and Uranyl Chloride with Benzoyl, Salicyloyl and Isonicotinoyl Hydrazines." Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry 16, no. 4 (January 1986): 513–25. http://dx.doi.org/10.1080/00945718608055925.

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6

Sano, Koji, Yoshihide Fujigaki, Takehiko Miyaji, Naoki Ikegaya, Kazuhisa Ohishi, Katsuhiko Yonemura, and Akira Hishida. "Role of apoptosis in uranyl acetate-induced acute renal failure and acquired resistance to uranyl acetate." Kidney International 57, no. 4 (April 2000): 1560–70. http://dx.doi.org/10.1046/j.1523-1755.2000.00777.x.

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7

Harris, J. Robin, Max Gerber, Wolfgang Gebauer, Wolfgang Wernicke, and Jürgen Markl. "Negative Stains Containing Trehalose: Application to Tubular and Filamentous Structures." Microscopy and Microanalysis 2, no. 1 (February 1996): 43–52. http://dx.doi.org/10.1017/s1431927696210438.

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Several examples are presented that show the successful application of uranyl acetate and ammonium molybdate negative staining in the presence of trehalose for TEM studies of filamentous and tubular structures. The principal benefit to be gained from the inclusion of trehalose stems from the considerably reduced flattening of the large tubular structures and the greater orientational freedom of single molecules due to an increased depth of the negative stain in the presence of trehalose. Trehalose is likely to provide considerable protection to protein molecules and their assemblies during the drying of negatively stained specimens. Some reduction in the excessive density imparted by uranyl acetate around large assemblies is also achieved. Nevertheless, in the presence of 1% (w/v) trehalose, it is desirable to increase the concentration of negative stain to 5% (w/v) for ammonium molybdate and to 4% for uranyl acetate to produce satisfactory image contrast. In general, the ammonium molybdate-trehalose negative stain is more satisfactory than the uranyl acetate-trehalose combination, because of the greater electron beam sensitivity of the uranyl negative stain. Reassembled taxol-stabilized pig brain microtubules, together with collagen fibrils, sperm tails, helical filaments, and reassociated hemocyanin (KLH2), all from the giant keyhole limpet Megathura crenulata, have been studied by negative staining in the presence of trehalose. In all cases satisfactory TEM imaging conditions were readily obtained on the specimens, as long as regions of excessively deep stain were avoided.

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8

Monshi, M. A. S., N. M. Abd El-Salam, and R. M. Mahfouz. "Isothermal decomposition of γ-irradiated uranyl acetate." Thermochimica Acta 322, no. 1 (November 1998): 33–37. http://dx.doi.org/10.1016/s0040-6031(98)00488-2.

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9

Takase, Hiroshi, and Makoto Sugiura-Nakazato. "PB-05 Recycling of Uranyl acetate solution." Microscopy 68, Supplement_1 (November 1, 2019): i48. http://dx.doi.org/10.1093/jmicro/dfz082.

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10

Tandler, Bernard. "Improved uranyl acetate staining for electron microscopy." Journal of Electron Microscopy Technique 16, no. 1 (September 1990): 81–82. http://dx.doi.org/10.1002/jemt.1060160110.

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11

Schneider, Jan Philipp, and Matthias Ochs. "Alterations of mouse lung tissue dimensions during processing for morphometry: A comparison of methods." American Journal of Physiology-Lung Cellular and Molecular Physiology 306, no. 4 (February 15, 2014): L341—L350. http://dx.doi.org/10.1152/ajplung.00329.2013.

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Preservation of original tissue dimensions is an essential prerequisite for morphometric studies. Shrinkage occurring during tissue processing for histology may severely influence the appearance of structures seen under the microscope and stereological calculations. Therefore, shrinkage has to be avoided so that estimates obtained by application of unbiased stereology are indeed unbiased. The present study investigates the alterations of tissue dimensions of mouse lung samples during processing for histology. Different fixatives as well as embedding protocols are considered. Mouse lungs were fixed by instillation of either 4% formalin or a mixture of 1.5% glutaraldehyde/1.5% formaldehyde. Tissue blocks were sampled according to principles of stereology for embedding in paraffin, glycol methacrylate without treatment with osmium tetroxide and uranyl acetate, and glycol methacrylate including treatment with osmium tetroxide and uranyl acetate before dehydration. Shrinkage was investigated by stereological measurements of dimensional changes of tissue cut faces. Results show a shrinkage of the cut face areas of roughly 40% per lung during paraffin embedding, 30% during “simple” glycol methacrylate embedding, and <3% during osmium tetroxide/uranyl acetate/glycol methacrylate embedding. Furthermore, the superiority of the glutaraldehyde-containing fixative regarding shrinkage is demonstrated. In conclusion, the use of a glutaraldehyde-containing fixative and embedding in glycol methacrylate with previous treatment of the samples with osmium tetroxide and uranyl acetate before dehydration is recommended for stereological studies of the mouse lung.

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12

Gorelik, V. S., A. A. Anik’ev, V. M. Korshunov, and Yu P. Voinov. "Probe Raman spectroscopy of sodium uranyl-acetate microcrystals." Optics and Spectroscopy 123, no. 2 (August 2017): 255–57. http://dx.doi.org/10.1134/s0030400x17080070.

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13

Soeldner, Al. "Uranium Is The Issue." Microscopy Today 6, no. 9 (November 1998): 24–25. http://dx.doi.org/10.1017/s1551929500069522.

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Uranium compounds, especially uranyl acetate, have been widely and routinely used as transmission electron microscopy centrist stains for biological materials since 1958. Those of us who do TEM of biologicals use small quantities of uranyl acetate, nitrate, formate, sulfate and perhaps other uranium compounds almost daily and therefore keep inventories of these salts and their solutions.In the 1980's growing concerns about medical and research wastes entering regional dump sites prompted state radiation officials in Oregon to begin tightening the regulations for monitoring and controlling all radioactive substances including the uranium compounds commonly used in processing biological specimens for TEM.

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14

PRASAD, Shiva, and Jaqueline Viana BARROS. "Electrometric studies on uranyl molybdates as a function of pH." Eclética Química 23 (1998): 59–70. http://dx.doi.org/10.1590/s0100-46701998000100005.

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The formation and composition of uranyl molybdates obtained by the interaction of uranyl acetate and sodium molybdate at specific pH levels 7.6, 5.5 and 4.1 have been studied by employing electrometric techniques involving pH and conductometric titrations. The results provide cogent evidence for the formation of three uranyl molybdates having the molecular formulae UO2O.MoO3, 3UO2O.7MoO3 and 2UO2O.8MoO3 in the vicinity of pH 5.7, 4.6 and 3.8, respectively. Analytical investigations of the compounds have also been carried out which substantiate the results of electrometric study.

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15

Perez, Evan, Cassandra Hanley, Stephen Koehler, Jordan Pestok, Nevo Polonsky, and Michael Van Stipdonk. "Gas Phase Reactions of Ions Derived from Anionic Uranyl Formate and Uranyl Acetate Complexes." Journal of The American Society for Mass Spectrometry 27, no. 12 (September 7, 2016): 1989–98. http://dx.doi.org/10.1007/s13361-016-1481-2.

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16

Hollóczki, Oldamur. "Uranyl(VI) Complexes in and from Imidazolium Acetate Ionic Liquids: Carbenes versus Acetates?" Inorganic Chemistry 53, no. 2 (December 30, 2013): 835–46. http://dx.doi.org/10.1021/ic402921b.

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17

Shepard, Nora, and Nelson Mitchell. "Simultaneous demonstration of matrix lipid and proteoglycan in rat growth plate through selective preservation: A new technique." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 404–5. http://dx.doi.org/10.1017/s042482010010408x.

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The presence of lipid in cartilage has been determined through biochemical analysis and associated with mineralization (1,2,3), as is proteoglycan (4,5). However, unlike proteoglycan, an ultrastructural technique does not exist for cartilage lipid stabilization.Nile blue sulphate, a dye used in fluorescence microscopy to study lipids, when added to the initial glutaraldehyde fixation and followed by potassium ferrocyanide reduced osmium and en bloc, uranyl acetate, retained lipids in the cartilage matrix. In addition to the lipid stabilization, matrix proteoglycan was also rendered insoluable, two matrix components that have not been observed simultaneously before.Slices of growth plate cartilage from Sprague Dawley rats were fixed in 2% glutaraldehyde/0.1M Na phosphate (pH 7.4) containing 0.1% nile blue (min.2 hrs.), rinsed (15 min.) with 0.1M Na phosphate buffer containing 0.2M sucrose and 0.1% nile blue,post fixed with 1% aqueous OSO4 containing 1.5% potassium ferrocyanide, briefly washed in 0.1M Na acetate buffer (pH 6.3) then stained with 0.25% uranyl acetate in 0.1M Na acetate for one hour.

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18

LIMA, Francisco José Santos, Luiz Henrique Medeiros da COSTA, and Ademir Oliveira da SILVA. "SPECTROCHEMICAL STUDIES OF THE ION UO22+ COORDINATED IN THE URANYL ACETATE UO2(H3CCOO)2.2H2O." Periódico Tchê Química 12, no. 24 (August 20, 2014): 33–46. http://dx.doi.org/10.52571/ptq.v11.n22.2014.33_periodico_22_pgs_33_46.pdf.

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The cation complex uranyl UO22+, is an molecular ion very stable. It possesses a linear geometry O=U=O, with connections U-O short of the order of 1,75 Å. Studies returned for to the fictionalization of this ion, for the insert of appropriate ligands it has been enough discussed in the literature. In this work we studied the transitions in the area of the uv-visible of the ion uranyl coordinated to the against-cation acetate, derived chemist of the acetic acid, in the compound UO2(H3CCOO)2.2H2O, known as uranyl acetate. For that your transitions were observed, calculated and compared by force of the oscillator by the Gaussian and Simpson methods. Optical parameters of connection such an as effect nephelauxetic (1 - ), covalence factor b1/2, and Sinha parameter, they were also appraised. Our purpose is allow information of this chemical system, for subsequent researches involving this ion in complex systems obtained in general by our group and groups of researches.

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19

Tian, Jing, Chunyan Li, Shaopu Liu, Zhongfang Liu, Jidong Yang, Jinghui Zhu, and Xiaoli Hu. "A rapid and highly sensitive fluorimetric method for the determination of meloxicam using uranyl acetate." Anal. Methods 6, no. 14 (2014): 5221–26. http://dx.doi.org/10.1039/c4ay00809j.

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20

Trapeznikova, Е. G., V. В. Popov, A. S. Radilov, and V. V. Shilov. "DOSE-DEPENDENT CHARACTER OF DISTURBANCE OF HEMATOPOIETIC AND IMMUNE SYSTEMS FUNCTION, PRODUCTION OF SOME HORMONES IN EXPERIMENTAL URANIUM ACETATE DIHYDRATE EXPOSURE." Toxicological Review 1, no. 1 (March 8, 2021): 14–19. http://dx.doi.org/10.36946/0869-7922-2021-1-14-19.

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The paper presents the results of an experimental study of the dose-dependent nature of functional changes in the body systems under chronic administration of uranyl acetate dihydrate in doses of 0.5 and 5.0 mg/kg per element for 18 weeks. The study was performed on 45 male outbred rats. It has been shown that uranyl acetate dihydrate in a dose of 0.5 mg/kg had no significant effect on hematological parameters. At the same time, activation of bactericidal activity of neutrophils, a decrease in the immunoregulatory index, and an increase in the blood concentration of tumor necrosis factor (TNF-α) have been revealed. The toxicant administered to rats in a dose of 5 mg/kg led to a decrease in the absolute number of erythrocytes, hemoglobin, hematocrit, platelets, the release of myelocytes into the blood, basophilia, monocytosis, the appearance of leukolysis cells and plasmatization of lymphocytes. On the part of the immune system, an increase in the biocidal capacity of neutrophilic granulocytes, TNF-α production, an increase in the number of CD8+ cells, and a reduction in the CD4+/CD8+ ratio have been found. Uranyl acetate dihydrate had a dose-dependent effect only on the number of cytotoxic T-lymphocytes, T-cells with the CD4+CD8+ phenotype, on the immunoregulatory index, and on the level of TNF-α. Hyperglycemia and glucosuria were also dose-dependent. An increase in glucose in the blood and urine indicated a violation of carbohydrate metabolism and kidney function. There was a decrease in the concentration of thyroxine, testosterone and an increase in the level of insulin. Uranyl acetate dihydrate led to the development of insulin resistance. The level of hormones did not depend on the dose of the toxicant administered to the animals.

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21

Stosman, К. I., К. V. Sivak, Т. A. Rassokha, and Т. N. Savateeva-Lubimova. "DOSE-DEPENDENT CHARACTER OF DISTURBANCE OF HEMATOPOIETIC AND IMMUNE SYSTEMS FUNCTION, PRODUCTION OF SOME HORMONES IN EXPERIMENTAL URANIUM ACETATE DIHYDRATE EXPOSURE." Toxicological Review 1, no. 1 (March 8, 2021): 20–26. http://dx.doi.org/10.36946/0869-7922-2021-1-20-26.

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The paper presents the results of an experimental study of the dose-dependent nature of functional changes in the body systems under chronic administration of uranyl acetate dihydrate in doses of 0.5 and 5.0 mg/kg per element for 18 weeks. The study was performed on 45 male outbred rats. It has been shown that uranyl acetate dihydrate in a dose of 0.5 mg/kg had no significant effect on hematological parameters. At the same time, activation of bactericidal activity of neutrophils, a decrease in the immunoregulatory index, and an increase in the blood concentration of tumor necrosis factor (TNF-α) have been revealed. The toxicant administered to rats in a dose of 5 mg/kg led to a decrease in the absolute number of erythrocytes, hemoglobin, hematocrit, platelets, the release of myelocytes into the blood, basophilia, monocytosis, the appearance of leukolysis cells and plasmatization of lymphocytes. On the part of the immune system, an increase in the biocidal capacity of neutrophilic granulocytes, TNF-α production, an increase in the number of CD8+ cells, and a reduction in the CD4+/CD8+ ratio have been found. Uranyl acetate dihydrate had a dose-dependent effect only on the number of cytotoxic T-lymphocytes, T-cells with the CD4+CD8+ phenotype, on the immunoregulatory index, and on the level of TNF-α. Hyperglycemia and glucosuria were also dose-dependent. An increase in glucose in the blood and urine indicated a violation of carbohydrate metabolism and kidney function. There was a decrease in the concentration of thyroxine, testosterone and an increase in the level of insulin. Uranyl acetate dihydrate led to the development of insulin resistance. The level of hormones did not depend on the dose of the toxicant administered to the animals.

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22

Chowdhury, Sanjib, Leonor Maria, Adelaide Cruz, Dario Manara, Olivier Dieste-Blanco, Thierry Stora, and António Gonçalves. "Uranium Carbide Fibers with Nano-Grains as Starting Materials for ISOL Targets." Nanomaterials 10, no. 12 (December 9, 2020): 2458. http://dx.doi.org/10.3390/nano10122458.

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This paper presents an experimental study about the preparation, by electrospinning, of uranium carbide fibers with nanometric grain size. Viscous solutions of cellulose acetate and uranyl salts (acetate, acetylacetonate, and formate) on acetic acid and 2,4-pentanedione, adjusted to three different polymer concentrations, 10, 12.5, and 15 weight %, were used for electrospinning. Good quality precursor fibers were obtained from solutions with a 15% cellulose acetate concentration, the best ones being produced from the uranyl acetate solution. As-spun precursor fibers were then decomposed by slow heating until 823 K under argon, resulting in a mixture of nano-grained UO2 and C fibers. A last carboreduction was then carried out under vacuum at 2073 K for 2 h. The final material displayed UC2−y as the major phase, with grain sizes in the 4 nm–10 nm range. UO2+x was still present in moderate concentrations (~30 vol.%). This is due to uncomplete carboreduction that can be explained by the fiber morphology, limiting the effective contact between C and UO2 grains.

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23

Gorelik, V. S., A. A. Anik’ev, V. M. Korshunov, Yu P. Voinov, and A. A. Loboyko. "Raman scattering and photoluminescence in sodium uranyl acetate polycrystals." Physics of Wave Phenomena 25, no. 4 (October 2017): 272–75. http://dx.doi.org/10.3103/s1541308x17040057.

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24

Ozmen, Murat, and Muhittin Yurekli. "Subacute toxicity of uranyl acetate in Swiss-Albino mice." Environmental Toxicology and Pharmacology 6, no. 2 (October 1998): 111–15. http://dx.doi.org/10.1016/s1382-6689(98)00025-8.

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25

Donova, Ilinka, Slobotka Aleksovska, and Viktor Stefov. "Synthesis, characterization and thermal decomposition of pyridinium uranyl acetate." Thermochimica Acta 348, no. 1-2 (April 2000): 169–74. http://dx.doi.org/10.1016/s0040-6031(00)00362-2.

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26

Gorelik, V. S., V. M. Korshunov, and Yu P. Voinov. "Resonance excitation of photoluminescence in sodium uranyl acetate crystals." Optics and Spectroscopy 121, no. 6 (December 2016): 819–22. http://dx.doi.org/10.1134/s0030400x16120122.

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27

Gorelik, V. S., A. A. Loboiko, and S. O. Nechipurenko. "Resonance Excitation of Photoluminescence in Crystalline Uranyl Acetate Dihydrate." Optics and Spectroscopy 124, no. 2 (February 2018): 221–26. http://dx.doi.org/10.1134/s0030400x1802008x.

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28

Fassel, Theresa A., and Marion L. Greaser. "Uranyl acetate as a primary fixative for skeletal muscle." Microscopy Research and Technique 37, no. 5-6 (June 1, 1997): 600–601. http://dx.doi.org/10.1002/(sici)1097-0029(19970601/15)37:5/6<600::aid-jemt21>3.0.co;2-s.

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29

Fan, Jinda, John J. Bozzola, and Yong Gao. "Encapsulation of Uranyl Acetate Molecules Using Hollow Polymer Templates." Journal of Colloid and Interface Science 254, no. 1 (October 2002): 108–12. http://dx.doi.org/10.1006/jcis.2002.8581.

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30

Czaban, B. Barbara, and Arthur Forer. "Visualization of ultraviolet microbeam irradiation sites with uranyl acetate." Journal of Microscopy 164, no. 1 (October 1991): 61–65. http://dx.doi.org/10.1111/j.1365-2818.1991.tb03192.x.

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31

MacCordick, H. J. "Complexes of uranyl formate and acetate with mycobactin S." Journal of Radioanalytical and Nuclear Chemistry Letters 128, no. 3 (October 1988): 249. http://dx.doi.org/10.1007/bf02166656.

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32

Murry, Maisha M., Stephen P. LaMont, Samuel E. Glover, and Henry B. Spitz. "In vitro dissolution of uranyl acetate using different methods." Journal of Radioanalytical and Nuclear Chemistry 296, no. 2 (September 15, 2012): 909–13. http://dx.doi.org/10.1007/s10967-012-2221-0.

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33

MacCordick, H. J. "Complexes of uranyl formate and acetate with mycobactin S." Journal of Radioanalytical and Nuclear Chemistry Letters 126, no. 2 (January 1988): 173–81. http://dx.doi.org/10.1007/bf02162436.

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34

Горелик, В. С., С. О. Нечипуренко, and А. А. Лобойко. "Зондовая спектроскопия комбинационного рассеяния света поликристаллов ураниловых соединений." Журнал технической физики 127, no. 10 (2019): 536. http://dx.doi.org/10.21883/os.2019.10.48353.164-19.

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AbstractRegularities in the Raman spectra of acetate uranyl compounds CsUO_2(CH_3COO)_3, CsUO_2(CD_3COO)_3, NaUO_2(CH_3COO)_3, NaUO_2(CD_3COO)_3, UO_2(CH_3COO)_2, and RbUO_2(CH_3COO)_3 have been analyzed. The Raman spectra are excited by 785-nm laser radiation and recorded using a miniature fiber-optic spectrometer with a multielement detector. It is established that the spectra of all these compounds contain a strong Raman satellite with a frequency in the range of 850–860 cm^–1. This satellite corresponds to symmetric stretching vibrations (type A _1) of the uranyl group. The obtained results make it possible to detect and analyze small amounts of uranyl compounds.

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35

Cross, R. H. M., A. N. Hodgson, and R. T. F. Bernard. "Uranyl acetate staining under different conditions of preparation, storage and use." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (August 12, 1990): 726–27. http://dx.doi.org/10.1017/s0424820100161199.

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Uranyl acetate is routinely used in the staining of thin sections of biological tissue for transmission electron microscopy. Although many methods for its preparation and use have been described, there is seldom reference to the reasons for variations in concentration, solvent, storage time and staining time. Likewise, possible variations in the effects of staining under different conditions are largely ignored. In order to gain clarity on this issue an attempt has been made to test three variables (solvent, storage time and use in light or dark) under controlled experimental conditions.The tissues used for the experiment were the testis of a marine limpet, the gut epithelium of a fresh-water catfish, and the kidney of a rat; all of which were fixed and embedded by standard methods. The uranyl acetate solutions were prepared at the outset of the experiment and dispensed into small volumes and stored in the dark at 4°C until required.

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36

Soga, Takeshi, and Ken Ohwada. "Resonance Raman excitation profile for uranyl acetate in dimethyl sulfoxide." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 55, no. 7-8 (July 1999): 1337–45. http://dx.doi.org/10.1016/s1386-1425(98)00306-0.

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37

Fonseca, Sofia M., Hugh D. Burrows, Maria G. Miguel, Mohamed Sarakha, and Mich�le Bolte. "Photooxidation of cellulose acetate and cellobiose by the uranyl ion." Photochemical & Photobiological Sciences 3, no. 3 (2004): 317. http://dx.doi.org/10.1039/b314671e.

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38

CHIEN, W. "Intrinsic hydration of monopositive uranyl hydroxide, nitrate, and acetate cations." Journal of the American Society for Mass Spectrometry 15, no. 6 (June 2004): 777–83. http://dx.doi.org/10.1016/s1044-0305(04)00088-1.

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39

Chien, Winnie, Victor Anbalagan, Melvin Zandler, Michael Stipdonk, Dorothy Hanna, Garold Gresham, and Gary Groenewold. "Intrinsic hydration of monopositive uranyl hydroxide, nitrate, and acetate cations." Journal of the American Society for Mass Spectrometry 15, no. 6 (June 2004): 777–83. http://dx.doi.org/10.1016/j.jasms.2004.01.013.

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Hartsock, Wendy J., Jennifer D. Cohen, and David J. Segal. "Uranyl Acetate as a Direct Inhibitor of DNA-Binding Proteins." Chemical Research in Toxicology 20, no. 5 (May 2007): 784–89. http://dx.doi.org/10.1021/tx600347k.

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Hosogi, Naoki, Hideo Nishioka, and Masamichi Nakakoshi. "Evaluation of lanthanide salts as alternative stains to uranyl acetate." Microscopy 64, no. 6 (September 14, 2015): 429–35. http://dx.doi.org/10.1093/jmicro/dfv054.

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Banerjee, Asok K., and Mihir Chowdhury. "Linear dichroism spectra of cubic sodium uranyl acetate single crystal." Chemical Physics Letters 139, no. 5 (1987): 421–25. http://dx.doi.org/10.1016/0009-2614(87)80584-5.

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Saha, U. "Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate." Talanta 37, no. 12 (December 1990): 1193–96. http://dx.doi.org/10.1016/0039-9140(90)80192-i.

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Rich, Steven A., M. Cristina Anzola, and William E. Gibbons. "Uranyl acetate staining of thick sections for electron microscope autoradiography." Journal of Electron Microscopy Technique 6, no. 3 (July 1987): 309–10. http://dx.doi.org/10.1002/jemt.1060060306.

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OSTA, Luiz Henrique Medeiros da, Francisco José Santos LIMA, and Ademir Oliveira da SILVA. "THERMAL STUDY AND STEREOCHEMICAL OF THE DI-HIDRATED URANYL ACETATE." Periódico Tchê Química 12, no. 23 (January 20, 2015): 66–73. http://dx.doi.org/10.52571/ptq.v12.n23.2015.66_p_23_pgs_66_73.pdf.

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In our studies we can infer the intensity of the interaction of these species through the decomposition temperature. It is believed that the greater the interaction, a higher outlet temperature. We performed molecular modeling to better visualize the chemical environment around the uranium and evaluate stereochemical data of the studied compound. Through modeling, we associate the distances connections with the strength of interactions between atoms and between molecules and is shown an inverse ratio bond distance and energy required for decomposing the species, in other words, the greater the distance, the lower the temperature decomposition. Also correlated to molecular modeling with bond breaking temperatures. With the results of thermal analysis we can see that there were two events. The first event occurred at a temperature varying between 84-163 °C (with a peak temperature of 121 °C) and there was a weight loss of 7.982%, which output is related to two water molecules. In the second event, there was a mass loss of 24.503%, which was assigned the output of acetates, which are coordinated to the uranyl ion.

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Caughey, R. C., and U. P. Kalyan-Raman. "Nonspecific structures seen in diagnostic muscle biopsies." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 846–47. http://dx.doi.org/10.1017/s0424820100128511.

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In a period of two years we have analyzed 50 muscle biopsies using the transmission electron microscope. Six nonspecific structures consisting of filamentous bodies, tubular aggregates, paracrystalline mitochondrial inclusions, honeycomb arrays, concentric laminated bodies, and finger print profiles were observed in 47 of 50 cases. In order to know the significance of these structures in muscle biopsies, we correlated their occurrence with their clinical history, histological findings, and histochemistry.The biopsies were initially fixed in 2.5% glutaraldehyde (pH. 7.5, 500 mOsm), then randomly minced and post fixed in 1% osmium tetroxide. All biopsies were processed with and without uranyl acetate en bloc staining in Walpole's buffer before ethanol dehydration. They were embedded in Epon 812 epoxy resin, sectioned, and stained with uranyl acetate and lead citrate before evaluation with a JEOL, JEM 100 C Transmission Electron Microscope. All grid squares of six different blocks were scanned to evaluate the ultra-structural pathology.

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Henry, Caroll E., T. L. Salaam, E. Steward-Clark, Joyce Craig, and Lennell Reynolds. "Characterization of Ustilago Hordei Fimbriae Using Scanning Transmission Electron Microscopy and Immunocytochemistry." Microscopy and Microanalysis 3, S2 (August 1997): 123–24. http://dx.doi.org/10.1017/s1431927600007509.

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Sporidia of Ustilago hordei produce surface fimbriae which are important in conjugation and pathogenicity. This work focuses on fimbrial origin and production using transmission electron microscopy (TEM) and immunocytochemistry.Wild type I4A sporidial cells cultured to log phase with rotary shaking in yeast extract glucose (YEG) growth for 48 h. at 21° C, were harvested by centrifugation at 8000 rpm, placed on formvar coated grids, negatively stained with 2% uranyl acetate and photographed in the JEOL 1200 STEM. Some cells were prepared for sectioning by fixation with gluteraldehyde and cacodylate buffer, post fixed in osmium tetroxide, dehydrated and embedded in epoxy and stained with uranyl acetate. The remainder of the cells were sheared in a blender to remove fimbriae. The defimbriated cells and 1 ml. of fimbrial suspension were presented to TEM. The rest of the fimbrial suspension was centrifuged at 30,000 rpm and he fimbrial pellet protein concentration was determined to be 1.345 nm. as assayed by UV adsorption.

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Rybicka, Krystyna. "Histogenesis of alveolar cell carcinoma." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 626–27. http://dx.doi.org/10.1017/s0424820100127566.

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Alveolar cell carcinoma (ACC) is a lung neoplasm characterized by the presence of lamellar bodies specific for normal type 2 alveolar cells. Tumor histogenesis is uncertain. The present study indicates that ACC originates from dedifferentiation of hyperplastic type 2 alveolar cells rather than migration of bronchial stem cells into alveoli as suggested earlier.An aliquot of human lung biopsy diagnosed as ACC was fixed in glutaraldehyde and osmium, treated with 1% aqueous uranyl acetate, and embedded in epoxy resin. Sections were stained for glycogen by periodic acid - thiosemicarbazide - silver proteinate, and post-stained by uranyl acetate and lead citrate.The tumor contained morphologically distinct cell clusters. Each cluster consisted of identical cells. Differences between clusters resulted from synchronous alterations in lamellar bodies, mitochondria, glycosomes, and ribosomes. These alterations revealed distinct stages in cell differentiation classified here as follows: stage 1-hyperplastic cells (Fig.1), stage 2-dedifferentiating cells (Fig.2), stage 3-undifferentiated cells (Fig.3), stage 4-differentiating cells (Fig.4).

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Segall, Iris, Olga L. Shaffer, Victoria L. Dimonie, and Mohamed S. El-Aasser. "Morphological study of structured latex particles by Transmission Electron Microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 882–83. http://dx.doi.org/10.1017/s0424820100150241.

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Transmission electron microscopy plays an important role in the study of the influence polymerization conditions have on the morphology of structured latex particles and thus in the understanding of the morphological effect of such particles on the structure-property relationships of polymeric end products.Structured latex particles are prepared by seeded emulsion polymerization, where the first stage is a polymerization of ”the core” poly(n-butyl acrylate) (PBA), followed by a second stage polymerization of ”the shell” poly(benzyl methacrylate/styrene) (P(BM/St)) at various ratios. The changes in polymerization conditions include such variables as the polymerization mode (batch vs. semicontinuous), core/shell ratio, shell thickness, and shell composition. Morphology studies of the structured latex particles are performed by transmission electron microscopy on preferentially stained samples. In a small vial, a few drops of latex sample are combined with a few drops of uranyl acetate (UAc) 2% solution. The uranyl acetate serves as a negative staining to better delineate the particles edges.

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Caughey, R. C., and C. E. Kelly. "Ultrastructural comparison of amyloid-like glyomerulopathy." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (August 12, 1990): 216–17. http://dx.doi.org/10.1017/s0424820100158625.

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Without transmission electron microscopy cases of amyloidosis-like glomerulopathy may be erroneously considered to be amyloidosis, diabetic or membranous nephropathy. Mesangial widening and increased matrix, and capillary basement membrane thickening are features common to amyloid-like glomerulopathy, amyloidosis, and diabetic renal problems. Amyloid-like immunofluorescence may be positive for IgG, and C3 within the mesangium and capillary walls suggesting possible membranous nephropathy. Some cases appear to represent light chain nephropathy, which is known to result in crystalline-like ultrastructural deposits. Identification of amyloid-like glomerulopathy is made by Congo Red negativity and characteristics of ultrastructural fibrils.The renal biopsies from all patients were fixed in 2.5% glutaraldehyde, rinsed in Millonig’s phosphate buffer, and post fixed in 1% osmium tetroxide. They were then en bloc stained with 1% uranyl acetate, rinsed with Walpole’s non-phosphate buffer, dehydrated with a graded series of ethanols and embedded with Epon 812 epoxy resin. Ultramicrotomy thin sections were post-stained with uranyl acetate and lead citrate and scanned using a JEOL JEM 100C.

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