Journal articles: 'Utility lines'

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Sie, L., S. Loong, and E. K. Tan. "Utility of lymphoblastoid cell lines." Journal of Neuroscience Research 87, no. 9 (July 2009): 1953–59. http://dx.doi.org/10.1002/jnr.22000.

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Drabich, P. P. "Investigation of long electrically conducting utility lines." Measurement Techniques 39, no. 3 (March 1996): 318–21. http://dx.doi.org/10.1007/bf02374587.

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Senior, G. "Clinical utility of the Rey tangled lines test." Archives of Clinical Neuropsychology 14, no. 8 (November 1999): 719. http://dx.doi.org/10.1016/s0887-6177(99)80194-2.

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Senior, G., M. Kelly, and L. Salzman. "Clinical utility of the Rey tangled lines test." Archives of Clinical Neuropsychology 14, no. 8 (November 1, 1999): 719. http://dx.doi.org/10.1093/arclin/14.8.719.

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Caldecott, R., M. Poirier, D. Scofea, D. E. Svoboda, and A. J. Terzuoli. "Underground mapping of utility lines using impulse radar." IEE Proceedings F Communications, Radar and Signal Processing 135, no. 4 (1988): 343. http://dx.doi.org/10.1049/ip-f-1.1988.0041.

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BOLAT, AHMET, and CANDACE A. YANO. "A surrogate objective for utility work in paced assembly lines." Production Planning & Control 3, no. 4 (October 1992): 406–12. http://dx.doi.org/10.1080/09537289208919410.

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Ajioka, J. "On the conceptual utility of current flow lines in waveguides." IEEE Transactions on Antennas and Propagation 34, no. 2 (February 1986): 219–27. http://dx.doi.org/10.1109/tap.1986.1143807.

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Marie Vincent, Krista, and Lynne-Marie Postovit. "Investigating the utility of human melanoma cell lines as tumour models." Oncotarget 8, no. 6 (January 2, 2017): 10498–509. http://dx.doi.org/10.18632/oncotarget.14443.

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Arndt, Channing, and Kenneth R. Simler. "Estimating Utility‐Consistent Poverty Lines with Applications to Egypt and Mozambique." Economic Development and Cultural Change 58, no. 3 (April 2010): 449–74. http://dx.doi.org/10.1086/650413.

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Pajaro, Adeloisa, Tony Nikoloutsopoulos, and Peter Roberts-Thomson. "Diagnostic utility of unidentified precipitin lines (UPLs) in immune precipitation assays." Pathology 47, no. 1 (January 2015): 62–67. http://dx.doi.org/10.1097/pat.0000000000000189.

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Kumar, Satish, Joanne E. Curran, David C. Glahn, and John Blangero. "Utility of Lymphoblastoid Cell Lines for Induced Pluripotent Stem Cell Generation." Stem Cells International 2016 (2016): 1–20. http://dx.doi.org/10.1155/2016/2349261.

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A large number of EBV immortalized LCLs have been generated and maintained in genetic/epidemiological studies as a perpetual source of DNA and as a surrogatein vitrocell model. Recent successes in reprograming LCLs into iPSCs have paved the way for generating more relevantin vitrodisease models using this existing bioresource. However, the overall reprogramming efficiency and success rate remain poor and very little is known about the mechanistic changes that take place at the transcriptome and cellular functional level during LCL-to-iPSC reprogramming. Here, we report a new optimized LCL-to-iPSC reprogramming protocol using episomal plasmids encoding pluripotency transcription factors and mouse p53DD (p53 carboxy-terminal dominant-negative fragment) and commercially available reprogramming media. We achieved a consistently high reprogramming efficiency and 100% success rate using this optimized protocol. Further, we investigated the transcriptional changes in mRNA and miRNA levels, using FC-abs ≥ 2.0 and FDR ≤ 0.05 cutoffs; 5,228 mRNAs and 77 miRNAs were differentially expressed during LCL-to-iPSC reprogramming. The functional enrichment analysis of the upregulated genes and activation of human pluripotency pathways in the reprogrammed iPSCs showed that the generated iPSCs possess transcriptional and functional profiles very similar to those of human ESCs.

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Vadivel, Karthi K. "Emergency Restoration of High Voltage Transmission Lines." Open Civil Engineering Journal 11, no. 1 (October 15, 2017): 778–85. http://dx.doi.org/10.2174/1874149501711010778.

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Background:Blackouts on high voltage (HV) transmission lines occur due to harsh weather and non-climatic reasons such as human errors and mechanical failures. While it is impossible to prevent all such situations, utility officials and contractors can be equipped to meet such emergencies.Objective:This paper discusses: (1) the economic impact of climatic events in the USA and economic justification for having Emergency Restoration (ER) plans and, (2) emergency restoration procedures for downed transmission lines in Oman. Key aspects of emergency restoration procedures are discussed.

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Knudsen, Erik S., Uthra Balaji, Brian Mannakee, Paris Vail, Cody Eslinger, Christopher Moxom, John Mansour, and Agnieszka K. Witkiewicz. "Pancreatic cancer cell lines as patient-derived avatars: genetic characterisation and functional utility." Gut 67, no. 3 (January 10, 2017): 508–20. http://dx.doi.org/10.1136/gutjnl-2016-313133.

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ObjectivePancreatic ductal adenocarcinoma (PDAC) is a therapy recalcitrant disease with the worst survival rate of common solid tumours. Preclinical models that accurately reflect the genetic and biological diversity of PDAC will be important for delineating features of tumour biology and therapeutic vulnerabilities.Design27 primary PDAC tumours were employed for genetic analysis and development of tumour models. Tumour tissue was used for derivation of xenografts and cell lines. Exome sequencing was performed on the originating tumour and developed models. RNA sequencing, histological and functional analyses were employed to determine the relationship of the patient-derived models to clinical presentation of PDAC.ResultsThe cohort employed captured the genetic diversity of PDAC. From most cases, both cell lines and xenograft models were developed. Exome sequencing confirmed preservation of the primary tumour mutations in developed cell lines, which remained stable with extended passaging. The level of genetic conservation in the cell lines was comparable to that observed with patient-derived xenograft (PDX) models. Unlike historically established PDAC cancer cell lines, patient-derived models recapitulated the histological architecture of the primary tumour and exhibited metastatic spread similar to that observed clinically. Detailed genetic analyses of tumours and derived models revealed features of ex vivo evolution and the clonal architecture of PDAC. Functional analysis was used to elucidate therapeutic vulnerabilities of relevance to treatment of PDAC.ConclusionsThese data illustrate that with the appropriate methods it is possible to develop cell lines that maintain genetic features of PDAC. Such models serve as important substrates for analysing the significance of genetic variants and create a unique biorepository of annotated cell lines and xenografts that were established simultaneously from same primary tumour. These models can be used to infer genetic and empirically determined therapeutic sensitivities that would be germane to the patient.

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Chandra, Vishal, Rajani Rai, and Doris Mangiaracina Benbrook. "Utility and Mechanism of SHetA2 and Paclitaxel for Treatment of Endometrial Cancer." Cancers 13, no. 10 (May 12, 2021): 2322. http://dx.doi.org/10.3390/cancers13102322.

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Endometrial cancer patients with advanced disease or high recurrence risk are treated with chemotherapy. Our objective was to evaluate the utility and mechanism of a novel drug, SHetA2, alone and in combination with paclitaxel, in endometrial cancer. SHetA2 targets the HSPA chaperone proteins, Grp78, hsc70, and mortalin, which have high mutation rates in endometrial cancer. SHetA2 effects on cancerous phenotypes, mitochondria, metabolism, protein expression, mortalin/client protein complexes, and cell death were evaluated in AN3CA, Hec13b, and Ishikawa endometrial cancer cell lines, and on growth of Ishikawa xenografts. In all three cell lines, SHetA2 inhibited anchorage-independent growth, migration, invasion, and ATP production, and induced G1 cell cycle arrest, mitochondrial damage, and caspase- and apoptosis inducing factor (AIF)-mediated apoptosis. These effects were associated with altered levels of proteins involved in cell cycle regulation, mitochondrial function, protein synthesis, endoplasmic reticulum stress, and metabolism; disruption of mortalin complexes with mitochondrial and metabolism proteins; and inhibition of oxidative phosphorylation and glycolysis. SHetA2 and paclitaxel exhibited synergistic combination indices in all cell lines and exerted greater xenograft tumor growth inhibition than either drug alone. SHetA2 is active against endometrial cancer cell lines in culture and in vivo and acts synergistically with paclitaxel.

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Godoy-Navarrete, F., F. G. Jiménez-Núñez, N. Mena-Vázquez, C. M. Romero-Barco, G. Diaz Cordoves, and A. Fernandez-Nebro. "FRI0039 LUNG ULTRASOUND UTILITY IN INTERSTITIAL LUNG DISEASE DETECTION IN RHEUMATOID ARTHRITIS." Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 594.2–594. http://dx.doi.org/10.1136/annrheumdis-2020-eular.5404.

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Background:Objectives:To analyze the diagnostic utility of lung ultrasound (US) to detect interstitial lung disease (ILD) in Rheumatoid arthritis (RA) patients comparing with high-resolution computed tomography (HRCT).Methods:Study design: We performed a cross-sectional, observational study in patients with RA-ILD (cases) controlled with a gruop of RA patients without ILD (controls) paired by sex, age and time of disease evolution.Protocol: Patients were selected between May and September 2019. Patients were interwied by two rheumathologist for the protocolized collection of clinical data. The patients were assessed using HRCT, Pulmonary Function Test (PFT) and lung US.. The rheumatology who performed the lung US were blinded to patients clinical data. Variables: (1) B-lines number; (2) evaluation of the lung- ultrasound score already described: L. Gargani, Gutiérrez comprehensivo, Gutiérrez reducido and Mohhammadi;(3)pleural irregularities; (4) A pattern US lost;(5). Other variables included demographic, clinical-analytical, therapeutic and ILD-type description. Statistical analysis: descriptive, bivariant analysis. We applied Pearson’s correlation coefficient between B-lines, PFT and clinical variable.Furthermore, to establish the cut-off point of the US B-lines number for detecting the presence of significant AR-ILD in relation to HRCT, we used the receiver operating characteristic (ROC) curve analysis. A logistic regression analysis was performed to identify the intercostal spaces (IV: B-lines number in each space) wich wereindependently associated with ILD (DV: ILD in HRCT).Results:71 patients were included, 37 (52,1%) with ILD-RA and 34 (47,95) RA controls. The main characteristics are shown in Table 1. RA-ILD presented more B-lines number than control without ILD (median ICR] 91.0 [31.0-149.0] vs 6.5 [1.5-30.5]; p=<0.001) and more pleural irregularities (PI) [PI-median(ICR) 41.0 (5.0-57.5) vs 2.5 (0.0-7.2); p<0,001]. Furthermore, RA-ILD showed a negative correlation between B-lines and DLCO(r =-0.337, p=0.048)and positive with DAS28 (r =0.347, p=0.035). Regarding US score, we found that the detection of 32.5 B-lines in 72 intercostal spaces, had aSensitivity of 75.7%, Specifity=79.4%, PPV= 80% and NPV=75%,whilst in reduced score of 10 intercostal spaces, the detection of 5.5 B-lines had a sensivity= 62.2%,Specifity= 91.3%, PPV=88.4%, NPV=69.5%. In multivariate analysis, the intercostal spaces which showed independent association with ILD were 3rdright anterior axillary space(OR [IC 95%] 19.0 [1.3-27.5]), 8thright posterior axillary space (OR [IC 95%] 0.04 [0.0-0.6]), 8thright subescapular space (OR [IC 95%] 16.5 [1.8-45.5]),9thright paravertebral space (OR [IC 95%] 7.11 [1.0-37.1]) and 2ndleft clavicular middle space(OR [IC 95%] 21.9 [1,26-37.8]).Conclusion:Lung Ultrasoud could be a useful tool for interstitial lung disease diagnosis associated with Rheumatoid Artrithis. A 10 space reduced score showed a similar total predictive capacity than 72-space scoreReferences:Disclosure of Interests:None declared

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XIONG, Fu-Li, and Hong-Sen YAN. "Optimally Sequencing Mixed-model Assembly Lines Optimally with Skip Utility Work Strategy." Acta Automatica Sinica 38, no. 2 (December 19, 2012): 259–69. http://dx.doi.org/10.3724/sp.j.1004.2012.00259.

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Lusa, L., V. Cappelletti, M. Gariboldi, C. Ferrario, L. De Cecco, J. F. Reid, S. Toffanin, et al. "Questioning the Utility of Pooling Samples in Microarray Experiments with Cell Lines." International Journal of Biological Markers 21, no. 2 (April 2006): 67–73. http://dx.doi.org/10.1177/172460080602100201.

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We describe a microarray experiment using the MCF-7 breast cancer cell line in two different experimental conditions for which the same number of independent pools as the number of individual samples was hybridized on Affymetrix GeneChips. Unexpectedly, when using individual samples, the number of probe sets found to be differentially expressed between treated and untreated cells was about three times greater than that found using pools. These findings indicate that pooling samples in microarray experiments where the biological variability is expected to be small might not be helpful and could even decrease one's ability to identify differentially expressed genes.

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Lusa, L., V. Cappelletti, M. Gariboldi, C. Ferrario, L. De Cecco, J. F. Reid, S. Toffanin, et al. "Questioning the utility of pooling samples in microarray experiments with cell lines." International Journal of Biological Markers 21, no. 2 (2006): 67–73. http://dx.doi.org/10.5301/jbm.2008.2616.

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Yan, Jingya, Siow Jaw, Kean Soon, Andreas Wieser, and Gerhard Schrotter. "Towards an Underground Utilities 3D Data Model for Land Administration." Remote Sensing 11, no. 17 (August 21, 2019): 1957. http://dx.doi.org/10.3390/rs11171957.

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With the pressure of the increasing density of urban areas, some public infrastructures are moving to the underground to free up space above, such as utility lines, rail lines and roads. In the big data era, the three-dimensional (3D) data can be beneficial to understand the complex urban area. Comparing to spatial data and information of the above ground, we lack the precise and detailed information about underground infrastructures, such as the spatial information of underground infrastructure, the ownership of underground objects and the interdependence of infrastructures in the above and below ground. How can we map reliable 3D underground utility networks and use them in the land administration? First, to explain the importance of this work and find a possible solution, this paper observes the current issues of the existing underground utility database in Singapore. A framework for utility data governance is proposed to manage the work process from the underground utility data capture to data usage. This is the backbone to support the coordination of different roles in the utility data governance and usage. Then, an initial design of the 3D underground utility data model is introduced to describe the 3D geometric and spatial information about underground utility data and connect it to the cadastral parcel for land administration. In the case study, the newly collected data from mobile Ground Penetrating Radar is integrated with the existing utility data for 3D modelling. It is expected to explore the integration of new collected 3D data, the existing 2D data and cadastral information for land administration of underground utilities.

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Zvi, Yoav, Elif Ugur, Brian Batko, Jonathan Gill, Michael Roth, Richard Gorlick, David Hall, et al. "Prognostic and Therapeutic Utility of Variably Expressed Cell Surface Receptors in Osteosarcoma." Sarcoma 2021 (February 2, 2021): 1–10. http://dx.doi.org/10.1155/2021/8324348.

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Background. Six cell surface receptors, human epidermal growth factor receptor-2 (Her-2), platelet-derived growth factor receptor-β (PDGFR-β), insulin-like growth factor-1 receptor (IGF-1R), insulin receptor (IR), c-Met, and vascular endothelial growth factor receptor-3 (VEGFR-3), previously demonstrated variable expression across varying patient-derived and standard osteosarcoma (OS) cell lines. The current study sought to validate previous expression patterns and evaluate whether these receptors offer prognostic and/or therapeutic value. Methods. Patient-derived OS cell lines (n = 52) were labeled with antibodies to Her-2, PDGFR-β, IGF-1R, IR, c-Met, and VEGFR-3. Expression was characterized using flow cytometry. The difference in geometric mean fluorescent intensity (geoMFIdiff = geoMFIpositive − geoMFInegative) was calculated for each receptor across all cell lines. Receptor expression was categorized as low (Q1), intermediate (Q2, Q3), or high (Q4). The event-free survival (EFS) and overall survival for the six cell surface receptors were estimated by the Kaplan–Meier method. Differences in hazard for EFS event and overall survival event for patients in each of the three expression levels in each of the six cell surface receptors were assessed using the log-rank test. Results. All 6 receptors were variably expressed in the majority of cell lines. IR and PDGFR-β expressions were found to be significant predictors for EFS amongst patients with nonmetastatic disease ( p = 0.02 and 0.01, respectively). The hazard ratio for EFS was significantly higher between high IR and intermediate IR expression (HR = 2.66, p = 0.02 ), as well as between high PDGFR-β and intermediate PDGFR-β expression (HR = 5.68, p = 0.002 ). Her-2, c-Met, IGF-1R, and VEGFR-3 were not found to be significant predictors for either EFS or overall survival. Conclusion. The six cell surface receptors demonstrated variable expression across the majority of patient-derived OS cell lines tested. Limited prognostic value was offered by IR and PDGFR-β expression within nonmetastatic patients. The remaining receptors do not provide clear prognostic utility. Nevertheless, their consistent, albeit variable, surface expression across a large panel of patient-derived OS cell lines maintains their potential use as future therapeutic targets.

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Khera, Pawan, Rachit Saxena, C. V. Sameerkumar, Kulbhushan Saxena, and Rajeev K. Varshney. "Mitochondrial SSRs and their utility in distinguishing wild species, CMS lines and maintainer lines in pigeonpea (Cajanus cajan L.)." Euphytica 206, no. 3 (July 7, 2015): 737–46. http://dx.doi.org/10.1007/s10681-015-1504-2.

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Radulović, Aleksandra, Dubravka Sladić, Miro Govedarica, Aleksandar Ristić, and Dušan Jovanović. "LADM Based Utility Network Cadastre in Serbia." ISPRS International Journal of Geo-Information 8, no. 5 (May 6, 2019): 206. http://dx.doi.org/10.3390/ijgi8050206.

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The utility network cadastre in Serbia is the main register of utility lines and the rights to them. The Law on State Survey and Cadastre states the necessity for implementing a unified information system of both a real estate and utility network cadastre, but this has not been achieved in practice. The reasons for such a unified information system are to ensure easier maintenance of the rights of both the utilities and properties located above or below them, to ensure more efficient procedures for obtaining the consent for placement, repair, or removal of the utility line, to prevent procedures being executed based on outdated data, to build an information system as the law prescribes, and to facilitate the business processes in the Serbian geodetic authority, since it is responsible for both registers. Therefore, an already-developed LADM (Land Administration Domain Model)-based country profile for Serbia should be extended to include information from the utility network cadastre. An analysis of Serbian legislation showed the necessity of extending the class set of the utility network cadastre by further specialization of the LADM LA_LegalSpaceUtilityNetwork class. Furthermore, such a system will support the maintenance of utility network data. In practice, when there is a change made on utility lines by the right holders, it is necessary to implement the change in the register. In many situations, this is not done, and the actual state does not correspond to the one in the register. Usually, modern technologies, such as ground penetrating radar (GPR) and LIDAR, are used for data acquisition in order to provide an update of the utility network data. Since these technologies produce 3D data, we analyzed how to link that data to the traditional 2D spatial paradigm.

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Cazzaniga, N. E., D. Pagliari, and L. Pinto. "PHOTOGRAMMETRY FOR MAPPING UNDERGROUND UTILITY LINES WITH GROUND PENETRATING RADAR IN URBAN AREAS." ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XXXIX-B1 (July 24, 2012): 297–302. http://dx.doi.org/10.5194/isprsarchives-xxxix-b1-297-2012.

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Cazzaniga, N. E., D. Carrion, F. Migliaccio, and R. Barzaghi. "A SHARED DATABASE OF UNDERGROUND UTILITY LINES FOR 3D MAPPING AND GIS APPLICATIONS." ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XL-4/W1 (May 6, 2013): 105–8. http://dx.doi.org/10.5194/isprsarchives-xl-4-w1-105-2013.

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Sinz, Michael, Devin Pray, and Judy Raucy. "The Utility of Stable Cell Lines to Assess Species Differences in PXR Transactivation." Drug Metabolism Letters 1, no. 2 (April 1, 2007): 147–52. http://dx.doi.org/10.2174/187231207780363561.

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Siddiqui, Surayya, Khandan Keyomarsi, and Savitri Krishnamurthy. "Utility of Collodion Technique for Creating Cell Blocks of Breast Cancer Cell Lines." Journal of the American Society of Cytopathology 1, no. 1 (November 2012): S12. http://dx.doi.org/10.1016/j.jasc.2012.08.019.

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Chiang, Shu-Yin. "Bernoulli serial production lines with quality control devices: Theory and application." Mathematical Problems in Engineering 2006 (2006): 1–30. http://dx.doi.org/10.1155/mpe/2006/81950.

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This paper develops the procedure for the analysis of the production systems with quality control devices. The evaluation of the production system requires an expression for the system performance measures as functions of the machine and buffer parameters. This paper presents a method for evaluating these functions and illustrates their practical utility using a case study at a production plant.

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Hudson, Jean L. "Additional Evidence for Gourd Floats on Fishing Nets." American Antiquity 69, no. 3 (July 2004): 586–87. http://dx.doi.org/10.2307/4128409.

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Goodwin, S. B., and I. Thompson. "Development of isogenic lines for resistance to Septoria tritici blotch in wheat." Czech Journal of Genetics and Plant Breeding 47, Special Issue (October 20, 2011): S98—S101. http://dx.doi.org/10.17221/3262-cjgpb.

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Septoria tritici blotch (STB), caused by the fungus Mycosphaerella graminicola (asexual stage: Septoria tritici), is one of the most economically important diseases of wheat worldwide. During the past decade 13 genes for resistance to STB have been identified and several molecular markers have been developed. However, analysis of resistance gene expression and utility for plant improvement programs would be increased if the resistance genes were isolated in a common susceptible background. To address this problem, a program was begun to backcross resistance genes Stb1–8 into two susceptible wheat cultivars. Work with genes Stb2, Stb3, Stb6 and Stb8 has proceeded the farthest. Resistance gene Stb3 is dominant, while Stb2 may be recessive. This will be the first report of recessive resistance to STB if confirmed. Molecular markers linked to the resistance genes are being validated in the backcross progeny and should provide the materials for efficient introgression of these genes into elite germplasm for future wheat improvement.

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Sepp, Katharine J., and Vanessa J. Auld. "Conversion of lacZ Enhancer Trap Lines to GAL4 Lines Using Targeted Transposition in Drosophila melanogaster." Genetics 151, no. 3 (March 1, 1999): 1093–101. http://dx.doi.org/10.1093/genetics/151.3.1093.

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Abstract Since the development of the enhancer trap technique, many large libraries of nuclear localized lacZ P-element stocks have been generated. These lines can lend themselves to the molecular and biological characterization of new genes. However they are not as useful for the study of development of cellular morphologies. With the advent of the GAL4 expression system, enhancer traps have a far greater potential for utility in biological studies. Yet generation of GAL4 lines by standard random mobilization has been reported to have a low efficiency. To avoid this problem we have employed targeted transposition to generate glial-specific GAL4 lines for the study of glial cellular development. Targeted transposition is the precise exchange of one P element for another. We report the successful and complete replacement of two glial enhancer trap P[lacZ, ry+] elements with the P[GAL4, w+] element. The frequencies of transposition to the target loci were 1.3% and 0.4%. We have thus found it more efficient to generate GAL4 lines from preexisting P-element lines than to obtain tissue-specific expression of GAL4 by random P-element mobilization. It is likely that similar screens can be performed to convert many other P-element lines to the GAL4 system.

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Nicolson, Norman G., Reju Korah, and Tobias Carling. "Adrenocortical cancer cell line mutational profile reveals aggressive genetic background." Journal of Molecular Endocrinology 62, no. 4 (May 2019): 179–86. http://dx.doi.org/10.1530/jme-18-0262.

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Adrenocortical carcinomas are rare tumors with poor prognosis and limited treatment options. Although widely used as in vitro models to test novel therapeutic strategies, the adrenocortical carcinoma-derived cell lines NCI-H295R and SW-13 have only partially been described genetically. Our aim was to characterize the mutational landscape of these cells to improve their experimental utility and map them to clinical subtypes of adrenocortical carcinoma. Genomic DNA from NCI-H295R and SW-13 cells was subjected to whole-exome sequencing. Variants were filtered for non-synonymous mutations and curated for validated adrenocortical and pan-cancer driver gene mutations. Genes mutated in the cell lines were mapped using gene ontology and protein pathway tools to determine signaling effects and compared to mutational and clinical characteristics of 92 adrenocortical carcinoma cases from The Cancer Genome Atlas. NCI-H295R and SW-13 cells carried 1325 and 1836 non-synonymous variants, respectively. Of these, 61 and 76 were known cancer driver genes, of which 32 were shared between cell lines. Variant interaction analyses demonstrated dominant TP53 dysregulation in both cell lines complemented by distinct WNT (NCI-H295R) and chromatin remodeling (SW-13) pathway perturbations. Both cell lines genetically resemble more aggressive adrenocortical carcinomas with worse prognosis, for which development of targeted therapies is most critical. Careful incorporation of the genetic landscapes outlined in this study will further the in vitro utility of these cell lines in testing for novel therapeutic approaches for adrenocortical malignancy.

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Soeda, Akio, Deric Park, and Toru Iwama. "STMC-04. THE UTILITY OF X0-GLIOMA STEM CELL LINES IN UNDERSTANDING OF GLIOMAGENESIS." Neuro-Oncology 18, suppl_6 (November 1, 2016): vi183. http://dx.doi.org/10.1093/neuonc/now212.768.

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Coffman, Frederick, Rachid Hamid, Marion C. Cohen, Ralph Garippa, and Stanley Cohen. "Biophysical Profiling of Tumor Cell Lines." Analytical Cellular Pathology 34, no. 5 (2011): 225–34. http://dx.doi.org/10.1155/2011/496874.

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Despite significant differences in genetic profiles, cancer cells share common phenotypic properties, including membrane-associated changes that facilitate invasion and metastasis. The Corning Epic® optical biosensor was used to monitor dynamic mass rearrangements within and proximal to the cell membrane in tumor cell lines derived from cancers of the colon, bone, cervix, lung and breast. Data was collected in real time and required no exogenously added signaling moiety (signal-free technology). Cell lines displayed unique profiles over the time-courses: the time-courses all displayed initial signal increases to maximal values, but the rate of increase to those maxima and the value of those maxima were distinct for each cell line. The rate of decline following the maxima also differed among cell lines. There were correlations between the signal maxima and the observed metastatic behavior of the cells in xenograft experiments; for most cell types the cells that were more highly metastatic in mice had lower time-course maxima values, however the reverse was seen in breast cancer cells. The unique profiles of these cell lines and the correlation of at least one profile characteristic with metastatic behavior demonstrate the potential utility of biophysical tumor cell profiling in the study of cancer biology.

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Gordon, Jenna, Mark Brown, and Melissa Reynolds. "Cell-Based Methods for Determination of Efficacy for Candidate Therapeutics in the Clinical Management of Cancer." Diseases 6, no. 4 (September 22, 2018): 85. http://dx.doi.org/10.3390/diseases6040085.

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Determination of therapeutic efficacy is a major challenge in developing treatment options for cancer. Prior to in vivo studies, candidate therapeutics are evaluated using cell-based in vitro methods to assess their anti-cancer potential. This review describes the utility and limitations of evaluating therapeutic efficacy using human tumor-derived cell lines. Indicators for therapeutic efficacy using tumor-derived cell lines include cell viability, cell proliferation, colony formation, cytotoxicity, cytostasis, induction of apoptosis, and cell cycle arrest. Cell panel screens, 3D tumor spheroid models, drug-drug/drug-radiation combinatorial analysis, and invasion/migration assays reveal analogous in vitro information. In animal models, cellular assays can assess tumor micro-environment and therapeutic delivery. The utility of tumor-derived cell lines for efficacy determination is manifest in numerous commercially approved drugs that have been applied in clinical management of cancer. Studies reveal most tumor-derived cell lines preserve the genomic signature of the primary tumor source and cell line-based data is highly predictive of subsequent clinical studies. However, cell-based data often disregards natural system components, resulting in cell autonomous outcomes. While 3D cell culture platforms can counter such limitations, they require additional time and cost. Despite the limitations, cell-based methods remain essential in early stages of anti-cancer drug development.

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Woldemichael, Girma M., James R. Vasselli, Roberta S. Gardella, Tawnya C. Mckee, W. Marston Linehan, and James B. McMahon. "Development of a Cell-Based Reporter Assay for Screening of Inhibitors of Hypoxia-Inducible Factor 2-Induced Gene Expression." Journal of Biomolecular Screening 11, no. 6 (June 7, 2006): 678–87. http://dx.doi.org/10.1177/1087057106289234.

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Reporter cell lines have been developed for the identification of inhibitors of gene expression enhanced by hypoxia-inducible factor 2, which has been implicated as a transcription factor involved in the tumorigenesis of clear cell renal carcinoma. Stably transformed reporter clones of the human renal clear cell carcinoma cell line 786-O were generated by transfection or retroviral infection. Luciferase reporter expression in the vectors used was driven by either the natural human vascular endothelial growth factor (VEGF) promoter-enhancer or by the VEGF and the human endothelial nitric oxide synthase enhancers modulating minimal human cytomegalovirus promoter. Utility of the generated reporter cell lines was validated by introducing the von Hippel-Lindau protein complex and testing for reporter inducibility by hypoxia. The dynamic range in reporter activity under hypoxic stress was found to be at least 30- to 40-fold, with a signal-to-noise ratio of 60:1. Properties of the cell lines such as tolerance to up to 3% DMSO, signal stability with multiple in vitro passages, and utility in both 96- and 384-well plate formats indicated their suitability for use in a high-throughput screen. In addition, the potential use of these reporter lines in the evaluation of high-throughput screening hits in vivo in various mice models has been demonstrated.

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Thawari, Atul Gajanan, Piyush Kumar, Rohit Srivastava, and Chebrolu Pulla Rao. "Lysozyme coated copper nanoclusters for green fluorescence and their utility in cell imaging." Materials Advances 1, no. 5 (2020): 1439–47. http://dx.doi.org/10.1039/d0ma00386g.

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Green fluorescent, pH dependent and water soluble copper nanoclusters (3–5 nm) were synthesized by stabilizing with lysozyme and these were demonstrated for imaging in both healthy and cancer cell lines as an alternate to green fluorescent protein.

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37

Kondo, Tadashi. "Current Status and Perspectives of Patient-Derived Models for Ewing’s Sarcoma." Cancers 12, no. 9 (September 4, 2020): 2520. http://dx.doi.org/10.3390/cancers12092520.

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Patient-derived cancer models, including cell lines, organoids, and xenografts, are indispensable tools in cancer research. These models, which recapitulate molecular features of original tumors, allow studies on the biological significance of cancer-associated genes, antitumor effects of novel agents, and molecular mechanisms underlying clinical behaviors of tumors. Moreover, the predictive utility of patient-derived cancer models is expected to facilitate drug development and precision medicine. Ewing’s sarcoma is a highly aggressive mesenchymal tumor with a high metastasis rate. Previous studies demonstrated the utility of cell lines and xenografts in Ewing’s sarcoma research and clinical studies. However, the number of Ewing’s sarcoma models available from public biobanks is limited; this creates an obstacle for research on Ewing’s sarcoma. Novel Ewing’s sarcoma models are needed to establish their utility, further our understanding of the molecular mechanisms, and help develop effective therapeutic strategies. In this review, the current status of patient-derived cancer models is overviewed, and future prospects of model development are discussed from the perspective of Ewing’s sarcoma research. It should be of interest to researchers and clinicians who work on patient-derived cancer models.

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Duan, Xu, Qi Dong, and Wanjun Ye. "Experimental Study on Seismic Performance of Prefabricated Utility Tunnel." Advances in Civil Engineering 2019 (October 27, 2019): 1–14. http://dx.doi.org/10.1155/2019/8968260.

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Utility tunnel is a kind of underground tunnel structure that carries more than two types of public utility lines, and the utility tunnels built by the prefabricated method have been adopted in many modern cities due to their easy maintenance and environmental protection capabilities. However, knowledge about the seismic performance of the prefabricated utility tunnel and pipelines inside is quite limited. In this paper, a prefabricated utility tunnel newly built in Xi’an, China, is taken as the prototype; a series of shaking table tests are conducted to investigate the seismic performance of the prefabricated utility tunnel in loess foundation, using El Centro earthquake wave as the input loading. Details of the experimental setup focus on the design of the soil container, scaled model (1 : 10), sensor arrangement, and test cases. Dynamic responses including evaluation of boundary effect, the amplification factor of the ground and structure, distribution of soil pressure, characteristics of predominant frequencies, and the damage phenomena are analyzed. Dynamic strain obtained by Fiber Bragg Grating sensors releases the critical positions of the prefabricated utility tunnel during the earthquake. Moreover, the dynamic responses of the pipelines contained in the utility tunnel are also analyzed. From aforementioned results, the seismic performance of the prefabricated utility tunnel has been revealed. The results will provide a reference for the seismic design of prefabricated utility tunnels.

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Bazuaye, Bright, and Alero I. Fenemigho. "Universal Jurisdiction Fault Lines and the Immunity of State Officials: A Salutary Warning Before Perdition." African Journal of International and Comparative Law 26, no. 4 (November 2018): 548–64. http://dx.doi.org/10.3366/ajicl.2018.0248.

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An examination of universal criminal jurisdiction (UCJ) utility reveals objections and obstacles. The African Union (AU) and many states are wary of its ascendancy. The gap between UCJ and its application is wide. Nevertheless, to prevent impunity for international crimes, a balanced and nuanced approach to UCJ is inevitable.

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Madan, S., and K. E. Bollinger. "Neural Network Based Power Flow Predictor." Journal of Advanced Computational Intelligence and Intelligent Informatics 4, no. 3 (May 20, 2000): 200–205. http://dx.doi.org/10.20965/jaciii.2000.p0200.

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This paper describes the development of a synergistic intelligent system for power system management. One component of this system is a neural network (NN) based software utility, which predicts power flows over transmission lines and transformers. This paper focuses on the NN predictor

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Tilden, D., W. Sierakowski, S. Cottrell, and H. Kim. "Utility Values Across Lines of Therapy in Immuno-Oncology Treatments: An Example from Advanced Melanoma." Value in Health 20, no. 9 (October 2017): A449. http://dx.doi.org/10.1016/j.jval.2017.08.289.

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Donovan, Brian T., Tania Bakshi, Sarah E. Galbraith, Christopher J. Nixon, Lisa A. Payne, and Stan F. Martens. "Utility of frozen cell lines in medium throughput electrophysiology screening of hERG and NaV1.5 blockade." Journal of Pharmacological and Toxicological Methods 64, no. 3 (November 2011): 269–76. http://dx.doi.org/10.1016/j.vascn.2011.09.002.

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Pilli, Tania, Kanteti V. Prasad, Shankar Jayarama, Furio Pacini, and Bellur S. Prabhakar. "Potential Utility and Limitations of Thyroid Cancer Cell Lines as Models for Studying Thyroid Cancer." Thyroid 19, no. 12 (December 2009): 1333–42. http://dx.doi.org/10.1089/thy.2009.0195.

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Helms, T. C., J. H. Orf, and R. A. Scott. "Predicted and actual yield advance in a bi-parental and backcross-derived soybean population." Canadian Journal of Plant Science 74, no. 1 (January 1, 1994): 15–18. http://dx.doi.org/10.4141/cjps94-004.

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Improved soybean [Glycine max (L.) Merr.] lines that include a plant introduction as one parent may be derived from a bi-parental (BP) or backcross (BC1) population. The number of BC1 families may be small in some instances because of a lack of successful crossing. The objectives of our experiment were to (1) compare the mean, genetic variance and distribution of lines from a BC1 population derived from only a few BC1 families with those from a BP-derived lines; (2) compare the BP and BC1 population mean with the mid-parent mean; and (3) evaluate the mean yield of the superior 10% of lines within each population. Two BC1F1 plants were selfed, and 75 random lines were derived from each plant. The mean yield, physiological maturity and plant height of BP-derived lines were greater than the mid-parent means. The genetic variance among BC1-derived lines was much smaller than among BP-derived lines. A utility function that combines mean yield and expected genetic gain identified the BC1 population as the superior population. Key words: Soybean, backcross, bi-parental, genetic gain

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Min, Guo, and Hong Kun Chen. "A Method of Drawing Contour Basing on Membrane Analogy Method." Advanced Materials Research 919-921 (April 2014): 2119–22. http://dx.doi.org/10.4028/www.scientific.net/amr.919-921.2119.

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It is common method that using landform key points and lines of landform to draw contours of large scale relief maps. In this paper ,It is discussed that using lamella analogue to draw contour of large scale relief maps is an another simple utility high effective method.

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Rivera-Báez, Lianette, Ines Lohse, Eric Lin, Shreya Raghavan, Sarah Owen, Ramdane Harouaka, Kirk Herman, et al. "Expansion of Circulating Tumor Cells from Patients with Locally Advanced Pancreatic Cancer Enable Patient Derived Xenografts and Functional Studies for Personalized Medicine." Cancers 12, no. 4 (April 20, 2020): 1011. http://dx.doi.org/10.3390/cancers12041011.

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Improvement in pancreatic cancer treatment represents an urgent medical goal that has been hampered by the lack of predictive biomarkers. Circulating Tumor Cells (CTCs) may be able to overcome this issue by allowing the monitoring of therapeutic response and tumor aggressiveness through ex vivo expansion. The successful expansion of CTCs is challenging, due to their low numbers in blood and the high abundance of blood cells. Here, we explored the utility of pancreatic CTC cultures as a preclinical model for treatment response. CTCs were isolated from ten patients with locally advanced pancreatic cancer using the Labyrinth, a biomarker independent, size based, inertial microfluidic separation device. Three patient-derived CTC samples were successfully expanded in adherent and spheroid cultures. Molecular and functional characterization was performed on the expanded CTC lines. CTC lines exhibited KRAS mutations, consistent with pancreatic cancers. Additionally, we evaluated take rate and metastatic potential in vivo and examined the utility of CTC lines for cytotoxicity assays. Patient derived expanded CTCs successfully generated patient derived xenograft (PDX) models with a 100% take rate. Our results demonstrate that CTC cultures are possible and provide a valuable resource for translational pancreatic cancer research, while also providing meaningful insight into the development of distant metastasis, as well as treatment resistance.

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Ye, Yuhuang, Yang Chen, Yongzeng Li, Ying Su, Changyan Zou, Yanping Chen, Lin Ou, Rong Chen, and Haishan Zeng. "Characterization and discrimination of nasopharyngeal carcinoma and nasopharyngeal normal cell lines using confocal Raman microspectroscopy." Spectroscopy 25, no. 5 (2011): 217–24. http://dx.doi.org/10.1155/2011/405457.

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Raman microspectroscopy can provide molecular-level information about the biochemical composition and structure of cells and tissues with excellent spatial resolution. In this study, Raman spectroscopy of individual cells from nasopharyngeal carcinoma cell lines C666-1, CNE2 and nasopharyngeal normal cell line NP69 are investigated for their differences. The spectral intensity ratio at 1449 and 1657 cm−1with a decision line of I1449/I1657=1.10 can very easily separate the tumor and normal cell lines into two groups. Principal component analysis (PCA) and linear discriminant analysis (LDA) are also used to classify different cell lines and achieved a specificity and sensitivity of 100 and 90%, respectively. The results support the potential utility of Raman spectroscopy for nasopharyngeal diagnosis.

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Osano, Bob. "Dynamo Theory, Nonlinear Magnetic Fields, and the Euler Potentials." Advances in Astronomy 2018 (October 10, 2018): 1–6. http://dx.doi.org/10.1155/2018/4823494.

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The debate on the role of Euler potentials (EP), α∇β, in the dynamo theory is a long-standing one. It is known that the cross-product of gradients of two the potentials may represent magnetic fields lines. However, 2D and 3D dynamo hydromagnetic simulations suggest that their utility as analogues of magnetic field potential is restricted. This raises questions about their utility in the broader context of magneto-genesis and dynamo theories. We reexamine this and find that a reinterpretation of such potentials offers a new insight into the role EP may play in the general evolution of magnetic fields.

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Kwinta, Andrzej, Karolina Ważydrąg, and Mariusz Zygmunt. "Analysis of power lines span geometry based on TLS measurements." E3S Web of Conferences 55 (2018): 00013. http://dx.doi.org/10.1051/e3sconf/20185500013.

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The inventory measurements of utility infrastructure are crucial issues in the field of engineering geodesy. Secure usage of power lines requires to monitor their geometry. According to classical approach to measurements of geometry of overhead power lines, it usually allows only to measure directly. Geodetic methods cannot be applied due to measuring limitations (lack of an appropriate area to conduct a measurement). Currently in engineering geodesy it is becoming more common to use methods of laser scanning as well as Unmanned Aerial Photogrammetric Survey. In the Paper, the procedure of determining of an overhead power line’s geometry is presented on the example of an one span measured with Terrestrial Laser Scanning methods. Field measurements were conducted by using laser scanner. Afterwards the point cloud was processed in order to receive the geometry of conductor. Finally, results were presented is tabular and graphic form.

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Toneguzzo, F., A. C. Hayday, and A. Keating. "Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells." Molecular and Cellular Biology 6, no. 2 (February 1986): 703–6. http://dx.doi.org/10.1128/mcb.6.2.703.

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The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

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Journal articles on the topic 'Utility lines'

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