Dissertations / Theses: 'Vamps'

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Petrescu, Mihaela. "Vamps, Eintaenzer, and desperate housewives social dance in Weimar literature and film /." [Bloomington, Ind.] : Indiana University, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3297124.

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Thesis (Ph.D.)--Indiana University, Dept. of Germanic Studies, 2007.
Title from dissertation home page (viewed Sept. 25, 2008). Source: Dissertation Abstracts International, Volume: 69-02, Section: A, page: 0622. Adviser: William Rasch.

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Wang, Guan. "Roles of substrate rigidity and composition in membrane trafficking." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCC195.

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Du cerveau à l’os, la rigidité et la composition de la matrice extracellulaire varient énormément et jouent un rôle dans les réponses cellulaires. La rigidité influe également sur la tension de la membrane plasmique, elle-même régulée par le trafic membranaire. Comment la rigidité et la composition du substrat peuvent réguler l'exocytose, qui à son tour régule la tension de la membrane, reste largement inconnu. Ici, j'ai utilisé l’imagerie pHluorin d’évènements uniques d’exocytose de cellules cultivées sur des substrats de rigidité et de composition contrôlée pour explorer la régulation de VAMP2 et VAMP7. J'ai développé un logiciel informatique pour identifier automatiquement les évènements de fusion, permettant une analyse rapide de données. J'ai contribué à l'étude montrant que l’exocytose VAMP7 est régulée par la kinase src, qui phosphoryle VAMP7 dans son domaine Longin (LD) (Burgo et al. JBC 2013). De plus, j’ai trouvé que la rigidité du substrat stimule l’exocytose, en présence de la laminine, de VAMP7, mais pas VAMP7 sans LD ni VAMP2. VAMP7 et VAMP7 sans LD sont par ailleurs également sensibles aux variations de la tension membranaire induites par chocs osmotiques. Enfin, j'ai identifié que LRRK1 est un partenaire du LD, et contrôle le transport rétrograde de VAMP7.Ces approches m’ont permis de révéler un nouveau mécanisme par lequel la rigidité, agissant sur la signalisation des intégrines, contrôle le transport de VAMP7 via LRRK1 et Rab21 (Wang et al. soumis). Ce mécanisme pourrait avoir un large intérêt potentiel pour comprendre la dynamique de la membrane dans des conditions normales et pathologiques, en particulier le cancer
From brain to bones, stiffness and composition of the extracellular matrix vary greatly and play a role in cell responses. Substrate rigidity also impacts plasma membrane tension, which has a close relationship with membrane trafficking. How substrate rigidity and chemistry sensing may regulate exocytosis, which in turn regulates membrane tension, is still largely unknown. Here, I used pHluorin imaging of single vesicle exocytosis in cells cultured on substrates of controlled rigidity and composition to explore the regulation of VAMP2 and VAMP7-mediated exocytosis. I developed a computer software to automatically identify fusion events thus allowing quick analysis of batch data. I contributed to the study showing that VAMP7 exocytosis is regulated by src kinase which phosphorylates VAMP7 in its Longin domain (LD) (Burgo et al. JBC 2013). I further found that VAMP7 but not VAMP7 lacking LD- or VAMP2-mediated secretion was stimulated by substrate stiffness on laminin. VAMP7 and VAMP7 lacking LD were similarly sensitive to osmotic chock-induced membrane tension changes. Finally, i showed that LRRK1, a regulator of egf receptor transport, is a partner of the LD, and controls the retrograde transport of VAMP7. These approaches allowed me to reveal a new mechanism whereby substrate rigidity, by acting on integrin signalling, enhances VAMP7 exocytosis via LRRK1- and Rab21-dependent regulation of its peripheral readily-releasable pool (Wang et al. submitted). This mechanism may have broad potential relevance for plasma membrane dynamics in normal conditions and diseases, particularly cancer

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Krapivkina, Julia. "Identification de protéines SNARE de l'exocytose des endosomes de recyclage dans les dendrites neuronales." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0343/document.

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Le trafic membranaire est un processus universel qui est essentiel pour la fonction neuronale dans un large spectre de fonctions. De la croissance neuronale et le développement morphologique à la libération des neurotransmetteurs et la plasticité synaptique, il prend en charge l'activité neuronale et donne d'innombrables questions qui animent la recherche sur la neurobiologie d'aujourd'hui. Notamment, l’exocytose des endosomes de recyclage (ER) dans les compartiments somatodendritiques participe à la transmission synaptique et à la potentialisation synaptique à long terme (PLT). Cependant la machine moléculaire sous-tendant l’exocytose des ER reste encore méconnue. Afin d’identifier les protéines SNAREs vésiculaires (v-SNARE) impliquées dans les différentes formes d’exocytose des ER postsynaptiques, nous avons d'abord imagé les protéines VAMP neuronales fusionnées avec la pHluorine, une GFP mutée sensible au pH dans les neurones de l’hippocampe en culture. Nous avons constaté que seulement VAMP2 et VAMP4, mais pas VAMP7, rapportaient des événements d’exocytose somatodendritique dans les neurones matures. Après avoir identifié ces deux protéines candidates, nous avons utilisé la combinaison de différentes techniques de régulation négative chronique ou aiguë pour désactiver leur fonction et observer les conséquences sur l’exocytose des ER, la transmission synaptique basale ou la PLT. Nos résultats suggèrent que VAMP2 est impliqué dans une forme d’exocytose régulée importante pour la PLT, mais pas l’exocytose constitutive des récepteurs AMPA, qui stabilise la transmission basale. VAMP4 est nécessaire pour l'exocytose constitutive d'une grande partie des endosomes, mais l'implication fonctionnelle de ces endosomes doit encore être explorée, car la régulation négative de VAMP4 ne modifie pas la transmission basale
Membrane trafficking is a universal process that is essential for neuronal function in a wide spectrum of applications. From neuronal growth and morphological development to neurotransmitter release and synaptic plasticity, it supports neuronal activity and gives countless questions that drive today’s neurobiology research. Notably, the trafficking of recycling endosomes (REs) in somatodendritic compartments participates in synaptic transmission and plasticity, such as long-term synaptic potentiation (LTP). However, the fusion machinery mediating RE exocytosis is still unclear. To identify the vesicular SNAREs (v-SNAREs) involved in different forms of postsynaptic RE exocytosis, we first imaged neuronal VAMP proteins fused with pH-sensitive pHluorin in cultured hippocampal neurons, and found that only VAMP2 and VAMP4, but not VAMP7, underwent somatodendritic exocytosis in mature neurons. After identifying these two candidate proteins, we used a combination of different downregulation techniques to chronically or acutely deactivate their function and observe consequences on REs exocytosis, basal synaptic transmission and LTP. Our results suggest that VAMP2 is involved in activity-regulated exocytosis important for LTP, but not constitutive postsynaptic AMPARs exocytosis, supporting basal transmission. VAMP4 is required for constitutive exocytosis of at least a large proportion of REs, but the functional implication of these endosomes still need to be explored, as VAMP4 downregulation did not alter basal synaptic transmission

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Lynch, Jordan. "Where Have I Known This Before? An Exploration of Harmony and Voice Leading in the Compositions of Chick Corea." Bowling Green State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1333991067.

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Jullié, Damien. "Les endosomes de recyclage fusionnent transitoirement avec la membrane plasmique des dendrites neuronales." Thesis, Bordeaux 2, 2012. http://www.theses.fr/2012BOR21942/document.

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Le trafic membranaire est essentiel dans les neurones pour la morphogénèse, le recyclage des vésicules synaptiques et des récepteurs. L’exocytose des récepteurs AMPA contenus dans les endosomes de recyclage (ER) est nécessaire pour l’expression de la plasticité synaptique à long terme (PLT). Pour étudier ce mécanisme, nous avons visualisé l’exocytose par microscopie de fluorescence sur des neurones en culture transfectés avec le récepteur de la transferrine (TfR), un marqueur des ER, fusionné à la phluorine. Un examen systématique des événements d'exocytose a révélé des différences de comportement. Dans la plupart des cas, les récepteurs diffusent rapidement dans la membrane plasmique après exocytose (discharge), mais dans environ 25% des cas, les récepteurs restent concentrés (display). L’utilisation de changements rapides de pH extracellulaire autour de la cellule montre que l’exocytose est transitoire : après quelques secondes (médiane 2.6s) les récepteurs sont réinternalisés. Ce mécanisme a pu être étendu aux récepteurs AMPA et β2-adrenergique, pour lesquels l’exocytose de type display avait déjà été décrite. L’imagerie deux couleurs montre que Rab11, un marqueur des ER, est enrichie au site d’exocytose. L’expression d’un dominant négatif de Rab11 connu pour inhiber la PLT provoque une diminution spécifique de la fréquence des évènements discharge. Dans nos recherches sur le mécanisme de l’exocytose, nous avons testé l’implication des protéines SNARE dans la fusion membranaire. Ainsi VAMP4 est enrichie avec le TfR dans les ER qui sont exocytés à une fréquence équivalente. De plus, elle est requise pour le recyclage du TfR
Membrane trafficking is essential for neuronal function: from growth of neurons and synapse formation to recycling of synaptic vesicles and receptors, questions concerning exocytosis and endocytosis are stimulating neurobiology research. In particular, trafficking of glutamate receptors present in recycling endosomes (REs) is necessary for the expression of long term potentiation (LTP). To investigate the mechanism of exocytosis in dendrites, we have imaged cultured rat hippocampal neurons transfected with transferrin receptor, a classical marker of REs, tagged with phluorin. As for AMPA receptors or β2-adrenegric receptors, single exocytic events has revealed two main behaviors: in most cases, receptors diffuse quickly in the plasma membrane after exocytosis (discharge events), but receptors can also remain clustered (display events). Using fast extracellular pH changes around the recorded cell, we show that for display events exocytosis is transient: after a few seconds (median 2.6 s) receptors are internalized. Moreover, using two color imaging of single exocytosis events with markers of neuronal compartments, we found that Rab11 is enriched at the exocytosis site, confirming the endosomal origin of the vesicles. Overexpression of a dominant negative form of Rab11 known to impair LTP decreases selectively the frequency of discharge events. As SNARE proteins are involved in virtually all membrane fusion processes, we investigated the role of Vamp proteins in somatodendritic exocytosis events. We found that Vamp4, unlike Vamp2 or Vamp7, is enriched in TfR containing compartments and can undergo exocytosis at high frequency and is required for TfR exocytosis

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Chaineau, Mathilde. "Régulation du trafic vésiculaire : rôle de la protéine Hrb dans l'endocytose de la SNARE vésiculaire TI-VAMP/VAMP7." Paris 6, 2009. http://www.theses.fr/2009PA066022.

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Le trafic membranaire assure la communication entre les différents compartiments intracellulaires ainsi qu’entre les cellules et leur environnement via l’exocytose et l’endocytose. Les protéines SNARE vésiculaires (v-SNARE) et leurs partenaires à la membrane cible (t-SNARE) sont responsables de la fusion membranaire. Des travaux antérieurs ont montré le rôle de la v-SNARE TI-VAMP dans une voie d’exocytose impliquée dans la croissance neuritique et le remodelage de la membrane plasmique. Le domaine amino-terminal Longin de TI-VAMP régule l'activité fusogénique de la protéine et son ciblage. J'ai montré que ce domaine interagit avec la protéine Hrb, un nouveau partenaire de TI-VAMP. Hrb est localisée dans des structures membranaires recouvertes de clathrine et régule l’internalisation de TI-VAMP. Le domaine Longin interagit également avec certains lipides, indiquant que ces derniers pourraient réguler le ciblage ou l'activité de TI-VAMP. Ces nouvelles données moléculaires et cellulaires établissent des liens moléculaires inédits entre une protéine responsable de la fusion membranaire, une protéine régulatrice de l'endocytose et les lipides.

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Gonzalez, i. Llinares Bernat. "Presynaptic mechanisms of short-term plasticity at hippocampal mossy fibersynapses." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0424/document.

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Les synapses fibres moussues de l‘hippocampe entre le gyrus denté et les cellulespyramidales de CA3 sont caractérisées par leur morphologie particulière, et par leurspropriétés distinctives de transmission synaptique et de plasticité présynaptique. Cessynapses sont parfois appelées «détonatrices» pour leur rôle fonctionnel dansl‘encodage de la mémoire épisodique. Cependant, les mécanismes moléculaires à labase des propriétés spécifiques de ces synapses restent peu connus. Ce travail estcomposé de deux parties principales:1) Phénotypage des synapses fibres moussues de l'hippocampe chez les sourisVAMP7 KOVAMP7 est une protéine SNARE vésiculaire de la famille des longins, qui joue unrôle dans la croissance des neurites durant le développement. Dans le cerveauadulte, VAMP7 est enrichi dans un sous-ensemble de terminaisons nerveuses, enparticulier dans les fibres moussues de l‗hippocampe. Nous avons analysé lafonction de VAMP7 dans la libération de neurotransmetteurs par une caractérisationextensive de la transmission synaptique et des mécanismes de plasticité de cettesynapse. L'absence de VAMP7 ne cause pas de graves déficits développementauxou neuronaux (Sato et al., 2011; Danglot et al., 2012). Les mécanismesprésynaptiques de la plasticité à court terme de la fibre moussue de l‘hippocampesemblent également normaux, pour des raisons éventuelles qui seront discutées.2) Circuits du CA3 examinés par traçage viral et enregistrements de pairesNous avons développé une technique pour établir des enregistrements en pairesentre cellules en grain du gyrus denté connectées et cellules pyramidales CA3 (GCCA3),sur des cultures organotypiques de tranches d'hippocampe de souris. Pouridentifier les partenaires présynaptiques directs à une cellule pyramidale CA3 ciblée,nous avons combiné l‘électroporation cellulaire unitaire et le traçage mono-transsynaptiquebasé sur un virus de la rage recombinant et pseudotypé. Nous avonstransfecté une cellule pyramidale CA3 unique par tranche avec les plasmides codantla glycoprotéine d‘enveloppe du virus de la rage (RG), un rapporteur fluorescent, etla protéine TVA (récepteur de surface apparenté au EnvA, qui n'a pas d‘homologuechez les cellules de mammifères). Les tranches ont ensuite été infectées avec levirus de la rage recombinant et pseudotypé. Après 3-4 jours, le traçage mono-transsynaptiquerévèle les entrées présynaptiques de ce neurone unique. Ensuite, nousavons pu établir des enregistrements de paires entre les cellules en grain-CA3connectés, ainsi que de quantifier les partenaires présynaptiques de la cellulepyramidale CA3 de départ
The hippocampal mossy fiber is characterized by its particular morphology, distinctsynaptic transmission and presynaptic plasticity. Moreover, this synapse has beencalled ―teacher‖ or ―detonator‖ for its proposed functional role in episodic memoryencoding. Nevertheless, the molecular mechanisms underlying its specific functionalproperties remain elusive. This work is composed of two main parts:1) Phenotyping Hippocampal Mossy Fiber Synapses in VAMP7 KO MiceVAMP7 is a vesicle SNARE of the longin family important in neurite growth duringdevelopment. In the adult brain, VAMP7 is enriched in a subset of nerve terminals,particularly at the hippocampal mossy fiber. We analyzed VAMP7 function inneurotransmitter release by characterizing basal and evoked transmission at thissynapse in KO mice and fully tested hypotheses relevant to short-term plasticity.Loss of VAMP7 has been previously reported not to cause major developmental orneurological deficits (Sato et al., 2011; Danglot et al., 2012). Presynapticmechanisms of short-term plasticity at the hippocampal mossy fiber also seemunaffected for potential reasons that will be discussed.2) CA3 Circuits Probed with RABV-Tracing and Paired RecordingsWe developed a technique to establish paired recordings between connected dentategyrus granule cells and CA3 pyramidal cells (GC-CA3) in mouse hippocampalorganotypic slice cultures. To identify direct presynaptic partners to a defined targetCA3 pyramidal cell, we combined single-cell electroporation (SCE) and mono-transsynaptictracing based on a pseudotyped, recombinant rabies virus (EnvApseudotyped RABV ΔG). Using SCE we transfected a single CA3 pyramidal cell perslice with the plasmids encoding: the RABV envelope glycoprotein (RG), afluorescent reporter, and TVA (the EnvA cognate surface receptor, which has nohomologue in mammalian cells). The slices were subsequently infected with EnvApseudotyped RABV ΔG. After 3-4 days, the RABV mono-trans-synaptic tracingrevealed the presynaptic inputs of that single neuron. Then, we were able toestablish paired recordings between connected GC-CA3 cells, as well as to quantifythe presynaptic partners of the starter CA3 pyramidal cell
De mosvezel van de hippocampus kenmerkt zich door een bijzondere morfologie,uitzonderlijke synaptische transmissie en presynaptische plasticiteit. De synapswordt ook wel "leraar" of "detonator" genoemd vanwege zijn waarschijnlijke rol in decodering van het episodisch geheugen. Toch blijven de specifieke moleculairemechanismen van dit synaps onbekend. Dit werk bestaat uit twee delen:1) Fenotypering van mosvezel synapsen van de hippocampus in VAMP7 KO muizenVAMP7 is een vesicle-SNARE van de longin familie van belang bij de groei vanneurieten tijdens de ontwikkeling. In de volwassen hersenen, wordt VAMP7 verrijkt ineen subset van zenuwuiteinden, vooral in de mosvezel van de hippocampus. Weanalyseerden VAMP7 functie in neurotransmitter afgifte door het karakteriseren vanbasale en opgeroepen transmissie bij deze synaps in KO muizen. Eerder is algesteld dat gebrek aan VAMP7 niet leidt tot grote ontwikkelings- of neurologischeafwijkingen (Sato et al., 2011; Danglot et al., 2012). Presynaptische mechanismenvan korte termijn plasticiteit in de mosvezel van de hippocampus lijken ookonaangetast te zijn, de mogelijke redenen hiervoor zullen worden besproken.2) CA3 circuits onderzocht met behulp van RABV-tracing en gekoppelde opnamesWe ontwikkelden een techniek om gekoppelde opnames tussen korelcellen van degyrus dentatus en aangesloten CA3 piramidale cellen (KC-CA3) op zogenaamde‗mouse hippocampal organotypic slice cultures‘ te meten. Om rechtstreeksepresynaptische partners te identificeren van een specifieke CA3 piramidale cel,combineerden we single-cell electroporation (SCE) en mono-trans-synaptic tracingop basis van een pseudo-typed, recombinant rabiësvirus (EnvA pseudogetypedRABV ΔG). Met behulp van SCE transfecteerde we één CA3 piramidale cel per slicemet plasmiden die coderen voor: het RABV glycoproteïne-envelop (RG), eenfluorescerende reporter, en TVA (de aan EnvA verwante oppervlakte receptor diegeen homoloog in zoogdiercellen heeft). De slices werden vervolgens geïnfecteerdmet ENVA pseudogetyped RABV ΔG. Na 3-4 dagen bracht de RABV mono-transsynaptischetracing de presynaptische ingangen van die ene neuron aan het licht.Hierna konden we gekoppelde opnames doen tussen verbonden KC-CA3 cellen.Daarnaast konden we de presynaptische partners van de starter CA3 pyramidale celkwantificeren

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Añaños, Gamarra Eduardo Martín, Burgos Piero Chichizola, Loro Abel Israel Colmenares, Rivera Gianella Chávez, and Guillén Ernesto Alonso Sosa. "¡Vamos app!" Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2021. http://hdl.handle.net/10757/656837.

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En esta investigación se analiza el desarrollo de la aplicación llamada “Vamos App”, que permite medir el aforo de los locales en tiempo real. La idea surge dada la coyuntura en la que se encuentra el país, donde estamos en un proceso constante de adaptación a las necesidades actuales, con aforos reducidos y distanciamiento social obligatorio. Además, el estilo de vida en general es cada vez más dinámico y la gente tiene menos tiempo para esperar. Por ello, esta aplicación permitirá a los usuarios conocer el aforo de los locales y conocer cuáles son los que tienen espacio disponible según la ubicación de la persona. Además, en nuestra versión “Premium” se podrá acceder a otros beneficios como descuentos, la posibilidad de hacer reservas, entre otros. La investigación nos ha permitido confirmar la viabilidad del proyecto, incursionando nuestra aplicación en el mercado peruano. Para ello, hemos utilizado las redes sociales como nuestro canal de ventas principal, lo que nos permitió obtener resultados satisfactorios expresados en las ventas realizadas. Posteriormente, se realizó el proceso de validación de problema y de la idea de negocio, para luego realizar los planes a futuro, con lo que se obtuvo el plan financiero. Los resultados de nuestra investigación se pueden resumir en que nuestro VAN es de 53,933.27, con retorno en el año 2 y nuestro TIR es de 57.70%, lo que demuestra la viabilidad del proyecto.
This research analyzes the development of the application called "Vamos App", which allows measuring the capacity of the premises in real time. The idea arises given the current situation in the country, where we are in a constant process of adaptation to current needs, with reduced capacity and mandatory social distancing. In addition, the lifestyle in general is increasingly dynamic and people have less time to wait. Therefore, this application will allow users to know the capacity of the premises and to know which ones have available space according to the location of the person. In addition, in our "Premium" version you can access other benefits such as discounts, the possibility of making reservations, among others. The research has allowed us to confirm the viability of the project, entering our application in the Peruvian market. For this, we have used social networks as our main sales channel, which allowed us to obtain satisfactory results expressed in the sales made. Subsequently, the process of validation of the problem and the business idea was carried out, to then carry out the future plans, with which the financial plan was obtained. The results of our research can be summarized in that our NPV is 53,933.27, with a return in year 2 and our IRR is 57.70%, which shows the viability of the project.
Trabajo de investigación

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Ortiz, Encalada Rafael. "¿Hacia dónde vamos?" IUS ET VERITAS, 2015. http://repositorio.pucp.edu.pe/index/handle/123456789/122522.

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Olmedo, Juan Pablo. "Probono : ¿Hacia dónde vamos?" IUS ET VERITAS, 2015. http://repositorio.pucp.edu.pe/index/handle/123456789/123142.

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Arnillas, Lafert Federico. "Presupuesto 2008, ¿cómo vamos?" Pontificia Universidad Católica del Perú, 2008. http://repositorio.pucp.edu.pe/index/handle/123456789/47390.

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Arndt, Andressa Dias. ""Mas, nós vamos compor?"." reponame:Repositório Institucional da UFSC, 2015. https://repositorio.ufsc.br/xmlui/handle/123456789/160559.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Psicologia, Florianópolis, 2015.
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Esta dissertação apresenta reflexões e análises de uma experiência musical coletiva, nomeada Roda de Música, realizada no contexto de um Centro de Referência da Assistência Social - CRAS, situado na região metropolitana de Curitiba. A pesquisa teve caráter participativo e comunitário e se configurou como um tipo de pesquisa-intervenção. Os temas aqui desenvolvidos nascem de inquietações em torno dos espaços criados para o fazer musical dentro do contexto da Proteção Social Básica. Por meio de uma relação dialógica entre a Psicologia Sócio-História e a Musicoterapia de base social e comunitária, buscou-se estabelecer construções teóricas que contribuam para a compreensão do fazer musical quando em processos comunitários. De igual modo, a descrição e a análise da experiência da Roda de Música, pretendeu abrir questões em torno do tipo de atuação dos profissionais em contexto socioassistencial. Este trabalho apresenta a música como mediadora de encontros que podem expandir as possibilidades de ser, sentir, agir e pensar dos sujeitos, sobretudo por meio de um tensionamento na configuração sensível. A partir dos encontros da Roda de Música os participantes puderam construir um cancioneiro com o repertório do grupo, dedicaram alguns encontros para a re-criação das canções escolhidas e envolveram-se em um processo criativo de composição. Eles utilizaram suas produções musicais para construção de visibilidades coletivas por meio do fortalecimento dos laços comunitários, bem como com a criação de relações outras com o território quando ocuparam o espaço urbano com suas sonoridades.

Abstract : This research presents some reflections and analysis from a colletive musical experience, named Music groups, held in the context of a Reference Center for Social Welfare - CRAS, located in the metropolitan region of Curitiba. This research realized was parcipatory and have community character, as well is configured as a kind of intervention research. The themes developed were born in concerns around the spaces created for the music making within the context of Basic Social Protection. Through a satisfactory relationship between the Socio-Historical Psychology and Social and Community Music Therapy, it attempted to establish theoretical constructs that contribute to understand what music does in those communitary processes. Similarly, the description and analysis of the experience of music groups, intended to open questions about the type of professional activities in social assistance context. This paper presents music as a mediator of meetings that would expand the possibilities of being, feeling, acting and thinking from the subjects, especially through a tension in the sensitive setting. From the Music Groups meetings participants were able to build a songbook with the repertoire of the group; some meetings were devoted to the re-creation of songs chosen and engaged in a creative process of composition. They used their musical production for construction of collective visibility through the strengthening of community ties and the creation of other relations with the territory when they occupied the urban space with their sounds.

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Kalmbach, Courtney Nicole Kalmbach. "Vamp Sans Amp." Miami University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=miami1470248833.

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Gomes, Ricardo César da Silva. "Álgebra ou geometria? Vamos a questão !" reponame:Repositório Institucional da UFC, 2016. http://www.repositorio.ufc.br/handle/riufc/16663.

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SILVA, Ricardo César da Silva. Álgebra ou geometria? Vamos a questão !. 2016. 54 f. Dissertação (Mestrado em Matemática em Rede Nacional) – Centro de Ciências, Universidade Federal do Ceará, Juazeiro do Norte, 2016.
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The present work has as its theme: Algebra or Geometry? Let the question! This study discusses mainly the relationship between these two distinct areas of the curriculum perspective, basic education. The goal is to show how tenuous, and at the same fruitful time, the border that separates the basic algebra and plane geometry, and how the teacher should investigate this boundary in the very first year of high school, even before submitting Analytic Geometry. This study is organized in the form of chapters, covering the following topics in order: why the mathematics teaching, the method used in problem solving as a process, the theoretical assumption, highlighting the similarity of triangles, the Pythagorean theorem, the laws of sines and cosines, the Ptolemy's theorem, and the list of proposed issues and an epistemological discussion of the proposed problems. The work was in the light of theoretical proposals Elon Lages Lima, Terence Tao and Paulo Freire, the latter a master of pedagogy. The survey was conducted exploratory and bibliographic way, qualitative character. Finally, the study aims to clarify that the curriculum division of mathematics classrooms in primary school in Algebra and Geometry is only one curriculum division and should not affect the view that all the contents studied are part of a whole perfectly consistent; given that, being on a math problem, students and teachers can make use of both tools of algebra as geometry of the results to solve it.
O presente trabalho tem como tema: Álgebra ou Geometria? Vamos à questão! Esse estudo discute, principalmente, a relação entre essas duas áreas distintas do ponto de vista curricular, no ensino básico. O objetivo é mostrar como é tênue, e ao mesmo tempo frutífera, a fronteira que separa a Álgebra básica e a Geometria Plana e, como o professor deve investigar esta fronteira logo nas primeiras séries do ensino médio, mesmo antes de apresentar a Geometria Analítica. Este estudo está organizado em forma de capítulos, abordando as seguintes temáticas em ordem: o porquê do ensino da Matemática, o método utilizado na resolução de problemas como processo, o pressuposto teórico, dando destaque à semelhança entre triângulos, ao teorema de Pitágoras, às leis dos Senos e Cossenos, ao teorema de Ptolomeu, e à lista de problemas propostos e uma discussão epistemológica dos problemas propostos. O trabalho foi à luz das propostas teóricas de Elon Lages Lima, Terence Tao e de Paulo Freire, este último um mestre da Pedagogia. A pesquisa foi feita de modo exploratório e bibliográfico, de caráter qualitativo. Por fim, o estudo pretende deixar claro que a divisão curricular das aulas de Matemática, no ensino básico, em Álgebra e Geometria é apenas um divisão curricular e não deveria afetar a visão de que todos os conteúdos estudados fazem parte de um todo perfeitamente coerente; tendo em vista que, estando diante de um problema de matemática, estudantes e professores podem lançar mão tanto de ferramentas da álgebra quanto de resultados da geometria para resolvê-lo.

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Krimmling, Jürgen, Ralf Franke, and Matthias Körner. "Verkehrsdatenaufbereitung und -modellierung im operativen Verkehrsmanagementsystem VAMOS." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-91192.

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Das Verkehrs-Analyse-, -Management- und -Optimierungs-System VAMOS nimmt die Aufgaben zur Datenaufbereitung als Grundlage für gezielte operative Verkehrsmanagementmaßnahmen im Ballungsraum Dresden wahr. Zur Modellierung von Verkehrs- und Infrastrukturdaten finden auf die Spezifika des Verkehrsgeschehens in urbanen Ballungsräumen zugeschnittene Ansätze Verwendung. Zur Verknüpfung von Verkehrssteuerungs- sowie Verkehrsinformationssystemen und dem Verkehrslagebild findet eine vorteilhafte Strategie zur Entkopplung von Datenerfassungs- und Steuerungssystemen erfolgreiche Anwendung
The operational traffic management system VAMOS realises specific data processing as general basis for aimed measures to influence traffic flow in the Dresden agglomeration. Approaches adapted to specific requirements of traffic activities in dense urban road networks were used for modelling traffic flow and infrastructural conditions. To annex traffic control systems as well as traffic information systems to the traffic conditions chart an advantageous strategy decoupling detection and control devices were implemented successfully

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Ospina, Álvarez Juan Sebastián. "Conversações hipervisuais: vamos falar sobre olhares masculinizados?" Universidade Federal de Goiás, 2018. http://repositorio.bc.ufg.br/tede/handle/tede/8267.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The present thesis intercrosses the visual cultures studies and the gender studies, having as object of analysis six encounters which made up the NarrATIVIDADES extension project. Genders Lab and narratives, which happened in November and December 2015, March and April 2017, in addition to the narratives produced by the people who committed themselves to the registered design from the School of Visual Arts (FAV) at the Pro-rector of Extension and Culture (PROEC) from the Federal University of Goiás (UFG). Masculinities in this study are treated as cultural constructs that are supported by the variety of speeches to operate in societies. For this reason, after reports of several visualities are problematized, these constructs of discipline and control that not only relate to the scope of the affective-sexual, but also to the domain of symbolic production. In this sense, this research craved analyze the possibilities and deconstruction strategies of visualities on masculinities and masculinization of looks, this from hypervisuals talks, works with memories and narratives produced by the group of students and teachers on the main subject of research: objectification and women hypersexualization, especially black women and black men; invisibility of the non heteronormative identities and androcentrism in using audiovisuals and media devices. The methodological approach nicknamed and used in this process corresponds to the hypervisual conversations that consist in an expansion of a narrative research expansion from works with visualities and countervisualities. To better comprehend the proper use of the term ‘hypervisuals’ are threshed, throughout the text, discussions around the concept of visual, visual images, the visualities, the countervisualities, the scopic regimes and many other terms of the studies of visual cultures. Since it’s a study linked to the Line of Image Cultural Research of image and Mediation Processes, this research outlines also the use of hypervisual narratives as possible to develop pedagogical processes with deconstructivist bias.
A presente tese entrecruza os estudos das culturas visuais e os estudos das relações dos gêneros, tendo como objeto de análise seis encontros que compuseram o projeto de extensão NarrATIVIDADES. Laboratório de gêneros e narrativas, os quais aconteceram em novembro e dezembro de 2015, março e abril de 2017, além das narrativas produzidas pelas pessoas que se vincularam ao projeto inscrito desde a Faculdade de Artes Visuais (FAV) ante a Pró-reitora de Extensão e Cultura (PROEC) da Universidade Federal de Goiás (UFG). As masculinidades neste estudo são tratadas enquanto construções culturais que têm como suporte os mais variados discursos para operar nas sociedades. Por este motivo, a partir dos relatos sobre múltiplas visualidades são problematizados esses construtos de disciplinamento e controle que não referem só ao âmbito do afetivo-sexual, mas também ao domínio da produção simbólica. Nesse sentido, esta investigação almejou analisar as possibilidades e estratégias de desconstrução das visualidades sobre as masculinidades e a masculinização dos olhares, isto a partir das conversações hipervisuais, trabalhos com memórias e as narrativas produzidas pelo grupo de estudantes e docentes sobre as temáticas principais da pesquisa: objetualização e hipersexualização das mulheres, principalmente das mulheres negras, e dos homens negros; invisibilização das identidades não heteronormativas e androcentrismo no uso de aparelhos e mídias audiovisuais. A abordagem metodológica alcunhada e usada neste processo corresponde às conversações hipervisuais que consistem em uma expansão da pesquisa narrativa a partir de trabalhos com visualidades e contravisualidades. Para compreender o uso do termo hipervisual são trilhadas, ao longo do texto, discussões em torno do conceito do visual, das imagens visuais, das visualidades, das contravisualidades, dos regimes escópicos e tantos outros termos caros aos estudos das culturas visuais. Por se tratar de um estudo vinculado à Linha de Pesquisa Culturas da Imagem e Processos de Mediação, esta investigação delineia, também, a utilização das narrativas hipervisuais como possível forma de desenvolver processos pedagógicos com viés desconstrutivista.

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Tonosaki, Lúcia Midori Damaceno. "Programa Vida Ativa Melhorando a Saúde (VAMOS)." reponame:Repositório Institucional da UFSC, 2016. https://repositorio.ufsc.br/xmlui/handle/123456789/172568.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Desportos, Programa de Pós-Graduação em Educação Física, Florianópolis, 2016.
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Programas de mudança de comportamento têm proporcionado melhora a saúde por intermédio da adoção de hábitos de vida saudáveis. Atentar-se para a percepção do sujeito tem um papel importante na compreensão da saúde, bem como os benefícios que a mudança de comportamento traz para as suas vidas. Nesse sentido, o objetivo deste estudo é analisar as percepções dos participantes no Programa Vida Ativa Melhorando a Saúde (VAMOS), quanto à promoção da saúde, barreiras e facilitadores. A pesquisa é caracterizada como qualitativa. Para compreender o objeto, este objetivo derivou de um estudo maior, que é o VAMOS, aplicado nos Centros de Saúde em Florianópolis (SC). A amostra foi constituída por todos os participantes do grupo de mudança do comportamento que concluíram o programa em suas duas versões: a primeira em 2012; e a segunda em 2014; e, que participaram do Grupo Focal (GF). Na primeira versão, 39 participantes iniciaram o Programa, desses, 16 participaram do GF. Na segunda versão, participaram 40 pessoas, desses, 19 do GF. Foi aplicada uma ficha diagnóstica aos participantes para formação do baseline, um GF, de onde foram extraídas as categorias sobre a percepção por meio da análise do conteúdo. Os dados sociodemográficos (sexo, estado civil, idade e renda) foram coletados por meio de entrevista face a face e transcritos e armazenados no Epidata. As variáveis qualitativas foram analisadas com auxílio do programa Nvivo 10 for Windows para criar as categorias de análise do conteúdo, para os dados sociodemográficos foi utilizada a análise descritiva desvio padrão e frequência pelo Programa Statistical Package for the Social Sciences (SPSS - versão 17.0®). O VAMOS possibilitou aos participantes a construção de processos cognitivos tanto individuais quanto coletivos. Estes processos cognitivos são fatores importantes para a tomada de atitudes que contribuem na mudança de comportamento, podendo contribuir na prevenção de problemas de saúde ou manutenção de uma vida saudável. A análise da percepção possibilitou uma avaliação sobre o programa, auxiliando as possíveis modificações de suas versões futuras. Assim, acredita-se que os achados da pesquisa possam contribuir na melhoria do programa, especialmente buscando potencializar os facilitadores e minimizar as barreiras para que mudanças de comportamento sejam alcançadas, que é a característica principal do programa.

Abstract : Behavior change programs have provided improved health through the adoption of healthy lifestyle habits. To be attentive to the perception of the subject is an important role for the understanding of health, as well as the benefits that the behavior change brings to their lives. In this sense, the objective of this study is to analyze the perceptions of participants of the Active Life Program Improving Health (VAMOS), as regards to health promotion, barriers and facilitators. The research is characterized as qualitative to better understand the study object, and was derived from a larger study, VAMOS, that is applied in health centers of Florianópolis (SC). The sample was consisted of all participants of the behavior change group that completed the program in their two versions: first in 2012, and second in 2014; and who participated in the Focus Group (FG). In the first version, 39 participants began the Program, of which these, 16 participated of the GF. In the second version, there were 40 people, of which, 19 participated of the GF. Diagnostic record was applied to the participants to form the baseline, one GF, where the categories of the perception were extracted using the content analysis. The socio-demographic data (gender, marital status, age and income) were collected using face-to-face interview, than were transcribed, and stored in Epidata. Qualitative variables were analyzed with the assistance of Nvivo10 program for Windows to create the content analysis categories; to the demographic data were used the descriptive analyzing, standard deviation and frequency, by the Statistical Package Program for Social Sciences (SPSS - version 17.0®). VAMOS enabled the participants to build both individual and collective cognitive processes. These are important factors for taking actions that contribute to the change behavior, and thus contribute to preventing health problems or to maintenance a healthy life. The analysis of the perception enabled an evaluation about the program, helping the possible changes for the future versions. In consequence, it is believed that the research findings can contribute to the improvement of the program, especially looking for enhance the facilitators and minimize barriers to behavior changes are achieved, the main characteristic of the program.

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Silva, Santa Julia. "“Vem vamos juntos! Dá-me tua mão e vamos juntos!”: reconhecimento e narrativas sobre a trajetória de Oliveira Silveira." Universidade Federal de Pelotas, 2014. http://repositorio.ufpel.edu.br/handle/ri/2690.

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Este estudo aborda numa perspectiva antropológica, a trajetória política e intelectual de Oliveira Silveira considerando um intelectual contemporâneo com uma significativa contribuição para a atualização do pensamento social brasileiro, por meio da elaboração de contranarrativas sobre a presença negra no país. As múltiplas frentes de atuação de Oliveira Silveira impulsionam a refletir sobre os atravessamentos identitários por meio dos quais este se constitui na pessoa-personagem narrada pelos interlocutores dessa pesquisa. Tomando como aporte as discussões que procuram atualizar o campo antropológico em termos teórico-metodológico, este trabalho se inspira na perspectiva de Sahlins (1990), Turner (2005) e Gonçalves (2010), autores que pensam a relação entre ação e estrutura, bem como a relação entre esquemas culturais prévios e as apropriações que os sujeitos fazem deles. Incorpora-se a perspectiva de Said (1993) que discute o papel do intelectual na sociedade contemporânea. Busca-se nos estudos pós-coloniais elementos convergentes para situar Oliveira Silveira como intelectual diaspórico e como portador de uma identidade híbrida representada pela articulação conjunta de sua identidade regional e de sua identidade negra. O estudo procura descrever os deslocamentos vivenciados por Oliveira Silveira tanto no sentido geográfico como sentido simbólico. Este foi o proponente do Vinte de Novembro como data de maior significado para a história negra do país, sendo também atuante em organizações negras, tais como: clubes sociais negros, escolas de samba, congadas. Além disso, organizou vários grupos de ativismo político na capital, o primeiro deles, o Grupo Palmares e depois o Grupo Semba, Razão Negra, Revista Tição e Associação Negra de Cultura. Todas as organizações lideradas por Oliveira Silveira se destinavam, simultaneamente, à luta política e à promoção da cultura negra. Essa dissertação se propõe a apresentar o percurso etnográfico percorrido para apreender como diferentes pessoas, que conviveram com Oliveira Silveira em diferentes momentos compreenderam, significaram e valorizaram a trajetória deste intelectual e ativista. A partir da proposta de etnografia multisituada de George Marcus (1994), a pesquisa procurou identificar a forma como cada interlocutor(a) elaborou sua percepção e interpretação a respeito da trajetória de Oliveira Silveira, convertendo-as em narrativas.
The presuppositions of this study address the political and intellectual trajectory of Oliveira Silveira based on an anthropological perspective. This study takes into consideration that he is a contemporary intellectual that provides a meaningful contribution to the update of the Brazilian social thought, through the presentation of counter-narratives related to the black presence in the country. The many places that Oliveira Silveira occupies stimulated the reflection about the identity crossings from which he is constituted in the person-character, narrated by his interlocutors.Taking as a guide to the discussions that focus on updating the anthropological field in theoretical-methodological terms, it was adopted in this study the perspectives of Sahlins (1990), Tunner (2005) e Gonçalves (2010), authors that think the relation between action and structure, as well as the place of the individual in the cultural life. In this study, it was incorporated the perspective of Said (1993) who discusses the role of the contemporary intellectual. Studies on convergent elements of the post-colonial period were also consulted in order to place Oliveira Silveira as a diasporic intellectual with a hybrid identity represented though the simultaneous articulation of his regional and black identity. In this study, it is intended to describe this displacement lived by Oliveira Silveira in the physical as well as in the symbolic way. This was the proponent of November twentieth as the date of greatest meaning to the black history of the country, being also active in black organizations, such as: clubs, samba schools and congadas. Besides, he has organized many groups in the capital, the first of them, the group Palmares and after the group Semba, Razão Negra, Revista Tição and Associação Negra de Cultura. All these organizations were lead by Oliveira Silveira and aimed at the political fight and to the promotion of the black culture. These dissertations aim at showing how different people, at different moments, understood, made sense and valued the intellectual trajectory of this activist. The choice of the topic of this study brings, a priori in an implicit way, the observation of the meaningful role played by Oliveira Silveira. This research aimed at identifying how each one chose to narrate their perception and comprehension related to the trajectory of Oliveira Silveira.

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Melo, Andre Veras de. "Desenvolvimento de controladores inteligentes para o sistema VASPS." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/265376.

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Orientador: Jose Ricardo Pelaquim Mendes
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecanica, Instituto de Geociencias
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Resumo: O cenario de producao em campos de petroleo tem apresentado nos anos recentes condicoes cada vez mais severas e complexas. Muitas descobertas em regioes de maior profundidade e com formacoes geologicas mais diversas tem demandado solucoes tecnológicas que beiram a fronteira do conhecimento, de forma que a producao tem andado lado a lado com o desenvolvimento de novas tecnologias. Assim, a necessidade do aumento do fator de recuperação para justificar os investimentos nestas condicoes sao desafios presentes nos novos projetos de pocos, de elevacao artificial e de recuperacao secundaria, o que vem levando ao desenvolvimento de solucoes submarinas e integradas, como o caso do separador submarino VASPS. No entanto, o controle de processos continuos, como a vazao de producao, a temperatura e a pressao, ainda e tratado da mesma forma que era feito quando do comeco da producao offshore em campos de oleo leve. Este trabalho visa permitir a melhoria de desempenho operacional do sistema VASPS, mensurada pelos custos totais gerados pelo sistema e pelos proventos gerados na producao de oleo e gas, bem como propor novas metodologias de sistemas de controle para sistemas similares, utilizando tecnologias recentes de controle inteligente. Vale ressaltar que o problema de controle e atualmente tratado em campo com solucoes de automacao, sem o estudo devido, causando danos a equipamentos que exigem intervencoes custosas. Os controladores propostos neste trabalho visam minimizar os esforcos aos quais estes atuadores estao expostos, provendo uma maior vida util as tecnologias de elevacao artificial de alta vazão e podendo ser aplicado de forma modular a outros processos relacionados.
Abstract: The oil production scenario has been becoming more and more complex and severe in the recent years. Plenty discoveries in deeper and more diversified formations have been requiring cutting edge technological solutions., such that the production has been deployed side by side with the technological development. Thus, the need for the enhancement of the recuperation factor in order to justify the investment in these new conditions are actual challenges within the recent design of wells, artificial lift systems and secondary recuperation projects, which has been leading to the development towards subsea integrated solutions, such as the VASPS subsea separator. However, the control of continuous processes, i.e. flow, temperature and pressure, is still dealt the same way it was done when the offshore production in light oil fields began. This work envisions the enhancement of the operational performance of the VASPS system, measured as a function of the total costs implied by the technology, altogether with the receipts generated in the gas and oil production, as well as the delivering of new methodologies for the design of controllers using late technologies of intelligent control. Also, it is worth recalling that nowadays the control task is dealt with automation solutions, without the proper handling, causing failures in equipments that require expensive rig intervention for workover. The controllers proposed in this work are designed to minimize the control effort, to which these actuators are exposed, providing extended lifetime to the high level production artificial lift technologies, being also able to be applied to other related processes in a modular form.
Mestrado
Explotação
Mestre em Ciências e Engenharia de Petróleo

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Amorim, Maria Alice. "Pelejas em rede: vamos ver quem pode mais." Pontifícia Universidade Católica de São Paulo, 2012. https://tede2.pucsp.br/handle/handle/4415.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The Cordel Literature, also known as string literature , constitutes one of the traditional poetical verses of Northeastern Brazil, characterized by the written production and specific typographic records. The communicational processes occupy, hence, a privileged position. In the last two decades, the cordelists have started performing through poetic contests by means of e-mail or chat communities on social networks, publishing what is called virtual battles , battles based on the cordel s imaginary battles, which can already be seen in titles of the 20th century. Thus, these written contests tradition harks back to the poetic challenges between two improvisers who would perform before an enthralled audience. The contests of cordelists and improvisers safe guard several codes and simultaneously interweave poetical elements which have been updated for centuries. Combining fixed forms, rhythm, themes, verbal duels, be they improvised or not, are recurrent in the poetry of cordelists, guitar players, coquistas, cirandeiros, maracatu masters, carnival ox, caboclinhos, rural carnival parades, samba of the countryside man. Face to face challenges, printed challenges, web-mediated challenges pave the way for the articulation of these expressions into a great poetical, oral text going through a continuous process in updating the virtual matrices. The research corpus harbors battle booklets (virtual and fictional); face-to-face challenges; poetry performing events; recorded virtual battles that took place on the web, cds and dvds by improvisers and cordelists. Participants observation, interviews and qualitative analysis of large texts that have been utilized in virtual contests (mediated by twitter, msn, facebook, orkut, sites, portals, email, and so forth) are in the methodology covering the fictional battles of cordelists and improvisers. Pursuant to the interdisciplinary concepts of communication by Jesús Martín-Barbero; tradition, performance and movement by Paul Zumthor; great oral text and virtual matrices by Jerusa Pires Ferreira; cyber-culture by Francisco Rüdiger, I propose to carry out a comparative study on the production of improvisers and cordelists spanning mainly the decades from 1990/2000. The purpose of the study is to investigate the existence of memories confluence and combination in the articulation of this large communication text in an ever adapting process, and also to speculate to what extent one can define such poetry as a recognizable cultural series
A literatura de cordel se constitui numa das poéticas tradicionais do Nordeste do Brasil, caracterizada por produção escrita e registro tipográfico específicos. Os processos comunicacionais ocupam, aí, posição privilegiada. Nas duas últimas décadas, os cordelistas passam a realizar disputa poética via e-mail ou bate-papo nas redes sociais, publicando o que denominam pelejas virtuais, calcadas nas pelejas imaginárias de cordel, que aparecem, já, em títulos do início do século XX. Por sua vez, a tradição dessas pelejas escritas remete ao desafio poético entre dois improvisadores, diante de uma platéia atenta. As pelejas de cordelistas e repentistas guardam variados códigos e simultaneamente entrelaçam elementos poéticos que se atualizam há séculos. Combinando formas fixas, ritmo, temas, os duelos verbais, de improviso ou não, são recorrentes na poesia de cordelistas, violeiros, coquistas, cirandeiros, mestres de maracatu, boi de carnaval, caboclinhos, blocos rurais de carnaval, samba de matuto. Em desafios ao vivo, desafios impressos, desafios mediados pela web, é possível articular essas expressões poéticas como um grande texto oral em contínuo processo de atualização de matrizes virtuais. No corpus da pesquisa, folhetos de pelejas (virtuais e fictícias); desafios ao vivo; eventos com performance de poesia; registro de pelejas virtuais ocorridas na web; cds e dvds de cordelistas e repentistas. Na metodologia, a observação participante, entrevistas e análise qualitativa do grande texto que se realiza nas pelejas virtuais (mediadas por twitter, msn, facebook, orkut, sites, portais, correio eletrônico etc.), nas fictícias pelejas de cordelistas e nas de improvisadores. Respaldada nos conceitos interdisciplinares de comunicação, de Jesús Martín-Barbero; tradição, performance e movência, de Paul Zumthor; grande texto oral e matrizes virtuais, de Jerusa Pires Ferreira; cibercultura, de Francisco Rüdiger, proponho-me realizar um estudo comparativo da produção de pelejas de cordelistas e repentistas, abrangendo sobretudo as décadas 1990/2000. O intuito é investigar a existência de confluências e combinações de memórias na articulação deste grande texto de comunicação, em movente processo adaptativo, e igualmente especular em que medida se pode definir tal produção poética como uma série cultural reconhecível

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Claudia, Rodrigues da Silva Ana. "Vamos maracatucá !!!: um estudo sobre os maracatus cearenses." Universidade Federal de Pernambuco, 2004. https://repositorio.ufpe.br/handle/123456789/1052.

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O maracatu cearense é uma manifestação cultural que representa um cortejo em homenagem aos Reis Negros. Esta dissertação pretende compreender como um bem cultural que exalta os negros se inseriu num Estado que disseminou uma ideologia de embranquecimento, considerando os negros ausentes da sua formação étnica / cultural. Pretende, ainda, apresentar o universo de dois grupos: o maracatu Rei de Paus e o Nação Baobab, ao detalhar as formas como seus brincantes dialogam com essa ideologia e qual a sua influência para a manifestação estudada. A dissertação reforça discussões sobre tradição, memória, identidade e cultura popular, através das interpretações dos sujeitos. Ela demonstra que é possível problematizar questões como políticas públicas e religião através de um bem cultural . Propõe a particularização dos maracatus, pois, a maioria dos estudos e pesquisas realizadas têm homogeneizado essa manifestação popular. Cada maracatu é um universo complexo precisando ser analisado no contexto em que está inserido. Nesse sentido, o maracatu do Ceará incorpora e ao mesmo tempo se contrapõe à ideologia do embranquecimento. Existe um jogo de identidades: a elite reforça a ausência dos negros, enquanto o cortejo coroa uma rainha negra, mesmo tendo o rosto pintado de preto, ela representa a realeza africana. O cortejo em seu conjunto resgata a história dos negros, suas alas representam personagens como: o casal de preto velho, os africanos, dentre outros

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Júnior, Luiz Carlos Pereira. "Avaliação da administração oral do Canabidiol em voluntátios sadios." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/60/60134/tde-27062013-154300/.

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Diante das evidências da existência de um sistema endocanabinóide em humanos, possivelmente disfuncional em transtornos de ansiedade e do crescente interesse terapêutico no uso do canabidiol (CBD), justifica-se um estudo da comparação entre as concentrações plasmáticas do CBD, administrados por via oral na forma de pó e dissolvido em óleo, para que se defina a forma de administração ideal do CBD e sua eficácia terapêutica na dose de 150mg, baseados em estudos cinéticos e dinâmicos (PK/PD). Neste contexto, o estudo investigou 39 voluntários sadios, alocados aleatoriamente em 3 grupos, que receberam 150 mg de CBD, em cápsulas na forma de pó (Grupo 1), dissolvido em óleo de milho (Grupo 2 ) e amido de milho (Grupo placebo). A concentração plasmática do CBD, por cromatografia gasosa acoplada a espectrometria de massas, foi determinada nos tempos: 0, 0,5, 1,0, 1,5, 2,0, 2,5, 3,0, 4,0 h, após a administração do fármaco. Os instrumentos de avaliação de medidas psicológicas foram: escala analógica de humor (VAMS) e a escala de identificação de ansiedade traço estado (IDATE) e nas medidas fisiológicas foram monitoradas a pressão arterial (PA) e a frequência cardíaca (FC). O teste ansiogênico aplicado foi o Teste de Simulação de Falar em Público (TSFP). No reconhecimento de expressões faciais (REF) foi realizada a tarefa Touch, em indicar a emoção apresentada na expressão do indivíduo. Nos resultados das medidas subjetivas (VAMS e IDATE), não se constatou o efeito ansiolítico; houve elevação da PA sistólica e diastólica e da FC; a intensidade da resposta no REF foi de 93% no Grupo 1 (pó) e de 81% no Grupo 2 (óleo). O pico plasmático médio foi de 20,70±1,68 ng/mL em 2,5h (CBD pó) e CBD em óleo de 82,59±9,52 ng/mL em 2h. Desta forma, este estudo PK/PD demonstrou que a forma farmacêutica ideal para a administração do CBD foi a em óleo e no estudo de dose /resposta, 150mg não possui eficácia terapêutico em relação a ansiedade.
In the face of the existence of an endocannabinoid system in humans, possibly dysfunctional in anxiety disorders and the growing interest in the therapeutic use of cannabidiol (CBD), justified a study comparing plasma concentrations of CBD, administered orally in powder form and dissolved in oil, in ordre to define the optimal mode of administration of the CBD and their therapeutic efficacy at a dose of 150mg, based on dynamic and kinetic studies (PK/PD). In this contexto, the study researched 39 healthy volunteers, allocated randomly in 3 groups, receiving 150 mg of CBD, capsules in powder form (Group 1), dissolved in corn oil (Group 2) and corn starch (placebo group). The plasmatic concentration of CBD was determined by gas chromatography coupled to mass spectrometry at the times: 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0 h, after administration of the drug. Assessment instruments of psychological measures were: analogical scale mood (VAMS) and scale identification of trait anxiety state (IDATE) and physiological measures were monitored in arterial pressure (PA) and heart rate (HR). The anxiogenic test applied was Simulated Public Speaking (TSFP). In recognition of facial expressions (REF) was performed the task touch, that consists in indicate the emotion shown the facial expression of the individual. The results of subjective measures (VAMS and IDATE) was not found the anxiolytic effect; there was increase of systolic and diastolic pressure and heart frequency; the response intensity in the REF was 93% in group 1 (powder) and 81% in Group 2 (oil). The peak mean plasma was 20.70 ± 1.68 ng / mL (CBD powder) in 2.5 h and 82.59 ± 9.52 ng / mL (CBD in oil) in 2h. Thus, this study PK / PD demonstrated that the ideal pharmaceutical form for administration of the CBD has been in oil and study of dose / response, 150mg has no therapeutic efficacy in relation to anxiety.

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Ferreira, Katherine. "Understanding the Role of Plasmodium falciparum VAMP8 SNARE Homologue." Honors in the Major Thesis, University of Central Florida, 2013. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1541.

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Malaria is one of the worlds most deadly infectious diseases and results in almost a million deaths each year, largely in children under the age of five in Sub-Saharan Africa. Outside Africa, malaria is responsible for a large number of cases in the Amazon rainforest of Brazil, Middle East, and in some areas of Asia [37]. According to the World Health Organization, there was an estimated 655, 000 deaths from malaria in 2012. Malaria is caused by a eukaryotic Apicomplexan parasite, Plasmodium, which has three distinct life cycles occurring in the midgut of the female Anopheles mosquito, the liver of the human host, and human erythrocytes. When the parasite infects the erythrocyte, some induced cell host modifications are made in order to accommodate growth. During its intra-erythrocytic life cycle, the malaria parasite traffics numerous proteins to a set of unique destinations within its own plasma membrane including the digestive vacuole, the apicoplast, rhoptries, and micronemes. Vesicular transport is an essential process in eukaryotic cells. This coordinated process is responsible for moving thousands of proteins between compartments within the cell. Essential to the targeting and fusion of protein transport vesicles in eukaryotes are SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors), a family of fusogenic proteins that are localized to distinct intracellular compartments [11]. Studies performed in our laboratory have identified 18 proteins putatively belonging to the PfSNARE family [2]. To date the exact role of PfSNAREs in the unique trafficking pathways of malaria is undetermined. Of particular interest to our study is PfVAMP8. In model eukaryotic organisms, VAMP8 containing vesicles deliver cargo to lysosomes and are involved in endocytosis. The food vacuole of the parasite is very similar to that of lysosomes and is essential to parasite survival. The study aims to identify the organelle(s) to which PfVAMP8 is localized and characterize membrane-association properties of this parasite’s R-SNARE protein. We believe that PfVAMP8 would localize to unique compartments in the parasite protein network flow. An in depth understanding of its mechanisms and localizations could be a key in developing novel anti-malarials. This study aims to identify the organelle(s) to which PfVAMP8 are localized, determine the trafficking determinants of this protein and determine this proteins’ expression and membrane association during the intra-erythrocytic stages of Plasmodium falciparum. Our immunofluorescence studies with known biological markers reveals that, PfVAMP8 passes through the endoplasmic reticulum, Golgi, and localizes to the food vacuole during trophozoite and schizont stage. Further characterization of the membrane association properties of the protein in this study reveals that PfVAMP8 is a soluble integral membrane protein with amphipathic characteristics.
B.S.
Bachelors
Burnett School of Biomedical Sciences
Molecular Biology and Microbiology

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GUIMARÃES, Juan Pablo Freire. "Vamos comer Oswald: por uma desterritorialização da antropofagia Oswaldiana." Universidade Federal do Pará, 2014. http://repositorio.ufpa.br/jspui/handle/2011/7525.

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CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Através de uma abordagem feita por viés histórico, estético, identitário e linguístico, esta dissertação apresenta uma reflexão sobre a proposição antropofágica elaborada por Oswald de Andrade, na sua relação com os discursos históricos, e com os discursos nacionalistas dos anos 30 no Brasil. A mesma demonstra como Oswald de Andrade constrói uma narrativa sobre a nacionalidade na qual a relação entre a identidade étnica e a nacional estão constante conflito. Partindo desse fato, a dissertação localiza duas maneiras pelas quais a antropofagia oswaldiana vem sendo narrada: uma que a territorializa no campo dos discursos nacionalistas, outras que tentam lhe retirar desse espaço, dando-lhe novos significados e potencialidades de transformação estética e sociocultural. Em outras palavras – lhe desterritorializando.

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Ortiz, de Zevallos Felipe, Cecilia Blume, Javier lguiñiz, and de Olarte Efraín Gonzales. "Perú y liberalismo económico: dónde estamos y adónde vamos." THĒMIS-Revista de Derecho, 2014. http://repositorio.pucp.edu.pe/index/handle/123456789/109826.

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En las páginas siguientes se presenta un tema innegablemente polémico -más aún en época de reformas constitucionales- puesto que forma parte de la discusión sobre cómo debemos ordenar nuestra economía y distribuir la riqueza. Y es que la discusión sobre la liberalización económica es particularmente trascendente en un país que ha experimentado las más variadas y graves formas de intervención estatal, así como sus más desastrosos efectos.

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Bleier, Michael, André Dias, António Ferreira, John Pidgeon, José Almeida, Eduardo Silva, Klaus Schilling, and Andreas Nüchter. "Real-time 3D Mine Modelling in the ¡VAMOS! Project." TU Bergakademie Freiberg, 2017. https://tubaf.qucosa.de/id/qucosa%3A23189.

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The project Viable Alternative Mine Operating System (¡VAMOS!) develops a new safe, clean and low visibility mining technique for excavating raw materials from submerged inland mines. During operations, the perception data of the mining vehicle can only be communicated to the operator via a computer interface. In order to assist remote control and facilitate assessing risks a detailed view of the mining process below the water surface is necessary. This paper presents approaches to real-time 3D reconstruction of the mining environment for immersive data visualisation in a virtual reality environment to provide advanced spatial awareness. From the raw survey data a more consistent 3D model is created using postprocessing techniques based on a continuous-time simultaneous localization and mapping (SLAM) solution. Signed distance function (SDF) based mapping is employed to fuse the measurements from multiple views into a single representation and reduce sensor noise. Results of the proposed techniques are demonstrated on a dataset captured in an submerged inland mine.

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Bleier, Michael, André Dias, António Ferreira, John Pidgeon, José Almeida, Eduardo Silva, Klaus Schilling, and Andreas Nüchter. "Real-time 3D Mine Modelling in the ¡VAMOS! Project." Technische Universitaet Bergakademie Freiberg Universitaetsbibliothek "Georgius Agricola", 2018. http://nbn-resolving.de/urn:nbn:de:bsz:105-qucosa-231296.

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The project Viable Alternative Mine Operating System (¡VAMOS!) develops a new safe, clean and low visibility mining technique for excavating raw materials from submerged inland mines. During operations, the perception data of the mining vehicle can only be communicated to the operator via a computer interface. In order to assist remote control and facilitate assessing risks a detailed view of the mining process below the water surface is necessary. This paper presents approaches to real-time 3D reconstruction of the mining environment for immersive data visualisation in a virtual reality environment to provide advanced spatial awareness. From the raw survey data a more consistent 3D model is created using postprocessing techniques based on a continuous-time simultaneous localization and mapping (SLAM) solution. Signed distance function (SDF) based mapping is employed to fuse the measurements from multiple views into a single representation and reduce sensor noise. Results of the proposed techniques are demonstrated on a dataset captured in an submerged inland mine.

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Carpier, Jean-Marie. "Rôle du traffic intracellulaire dans la signalisation et la réponse lymphocytaire T." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB242/document.

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Une réponse immunitaire efficace contre un large spectre de pathogènes ou contre des cellules tumorales nécessite l’activation des lymphocytes T CD4+. L’engagement du récepteur T par un peptide antigénique apprêté sur les produits de classe-II du complexe majeur d’histocompatibilité (peptide-CMH, pCMH) portés par une cellules présentatrices d’antigène (CPAg), conduit à de nombreux remaniements du lymphocyte T. Il s’établit notamment à l’interface entre le lymphocyte T et la CPAg, une structure spécialisée qui est la synapse immunologique (ou synapse immune). La synapse est le siège d’événements de signalisation intenses où diverses molécules de signalisation nécessaires l’amplification et la diversification du signal provenant du TCR sont recrutées. Ces évènements de signalisation sont régulés par l’adaptateur transmembranaire LAT (« Linker for Activation of T cells ») qui est présent à la membrane plasmique ainsi que dans des compartiments intracellulaires. La fraction intracellulaire de LAT est recrutée à la synapse immune et il est proposé que ces compartiments vésiculaires participent à la signalisation lymphocytaire T. L’objectif de ce travail de thèse a été de déterminer les voies de trafic intracellulaire nécessaires au recrutement de la fraction intracellulaire de LAT à la synapse immunologique et de comprendre le rôle de ce transport dans l’activation et la réponse lymphocytaire T. Par des approches d’extinction de l’expression de différentes molécules de transport intracellulaires dans les cellules T Jurkat ou des lymphocytes T CD4+ primaires humains ou par l’utilisation de souris Knock-Out (KO), nous avons mis en évidence plusieurs voies de transport impliquées dans le transport de LAT. Nous avons ainsi mis en évidence que le recrutement de LAT à la synapse immunologique nécessite une voie de sécrétion dépendante de la protéine SNARE vésiculaire VAMP7. L’analyse plus avant du transport de LAT a par ailleurs permis de montrer que LAT est présente dans des compartiments de recyclage alors que VAMP7 est principalement localisée dans l’appareil de Golgi. L’étude de la petite GTPase Rab6 et de la protéine t-SNARE syntaxine-16 qui sont impliquées dans des voies de transport rétrograde entre les endosomes de recyclage et l’appareil de Golgi, a permis de dévoiler que cette voie de transport est requise dans le recrutement de LAT à la synapse immunologique, ainsi qu’à la réponse lymphocytaire T in vitro, ex vivo et in vivo. Enfin, le rôle de la protéine de transport intraflagellaire IFT20, qui a déjà été mis en cause dans le transport du TCR, a été analysé chez la souris et a également montré des défauts de recrutement de LAT à la synapse et dans l’activation lymphocytaire T ex vivo et in vivo. Nos résultats mettent ainsi en évidence que la régulation du transport intracellulaire dans les lymphocytes T joue un rôle crucial dans l’activation lymphocytaire T. Nous proposons ainsi un modèle dans lequel LAT est constitutivement internalisé depuis la membrane plasmique et poursuit une voie de recyclage dépendante de l’appareil de Golgi qui contient la machinerie de sécrétion associé à VAMP7. Cette voie de transport intracellulaire, conditionnerait la resécrétion polarisée de LAT à la synapse immunologique dans les conditions d’activation et une réponse lymphocytaire T robuste
The immune response against a broad spectrum of pathogens or against tumor cells requires the CD4 + T lymphocytes activation. The triggering of T Cell Receptor (TCR) by peptide-MHC through antigen-presenting cells (APC) leads to numerous T-cell remodeling and the establishment of a specialized structure at the interface between the T lymphocyte and the APC: the immunological synapse. The synapse is the site of intense signaling events where various signaling molecules are recruited in order to amplify and diversify the signal initiated by the TCR. These signaling events are regulated by the transmembrane adapter LAT ("Linker for activation of T cells") which is present at the plasma membrane as well as in intracellular compartments. The intracellular fraction of LAT is recruited at the immune synapse and it is proposed that these vesicular compartments participate in T lymphocyte signaling. The objective of this thesis work was to determine the intracellular trafficking pathways required for the recruitment of the intracellular pool of LAT to the immunological synapse and understand the role of this transport in T cell activation and response. By silencing the expression of different intracellular transport molecules in Jurkat T cells or primary human CD4 + T lymphocytes, or by using Knock-Out (KO) mice, we have highlighted several trafficking pathways involved in the transport of LAT to the immune synapse. We have demonstrated that the recruitment of LAT to TCR activation sites requires a secretion pathway dependent on the vesicular SNARE protein VAMP7. Further analysis of the transport of LAT showed that LAT is present in recycling compartments whereas VAMP7 is mainly located in the Golgi apparatus. The study of the small GTPase Rab6 and the t-SNARE syntaxin-16 protein that are involved in the retrograde transport pathways between the recycling endosomes and the Golgi apparatus, demonstrated that this route of transport is required for the recruitment of LAT to the immunological synapse and for T lymphocyte response in vitro, ex vivo and in vivo as well. Finally, the role of intraflagellar transport protein IFT20, which has already been implicated in the transport of TCR, was analyzed in mice and also showed defects in LAT recruitment to synapse and T lymphocyte activation ex vivo and in vivo. Our results thus show that the regulation of intracellular transport plays a crucial role in T lymphocyte activation. We thus propose a model in which LAT is constitutively internalized from the plasma membrane and pursues a Golgi-dependent recycling pathway that contains the secretion machinery associated with VAMP7. This intracellular transport pathway would thus allow the polarized LAT re-secretion to the immunological synapse under activation conditions and a robust T lymphocyte response

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Trevisani, Marcel Montels. "Avaliação de uma nova estratégia vacinal para a prevenção da Rodococose equina." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/17/17147/tde-04082015-155605/.

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Infecções pulmonares de potros jovens por Rhodococcus equi resultam em grave pneumonia levando à morte um grande número de animais todos os anos. Até o momento não há nenhuma vacina aceita globalmente para a prevenção da rodococose equina. O único tratamento preconizado é baseado em antibioticoterapia, porém os protocolos clínicos são longos, de alto custo, com efeitos colaterais e tem favorecido a seleção de cepas resistentes aos antibióticos. Diversas estratégias no desenvolvimento de uma vacina segura e eficiente contra a rodococose foram propostas, porém, não induziram um efeito protetor considerável. O principal fator de virulência do R. equi descrito e amplamente estudado é a proteína vapA, no entanto, outras proteínas localizadas na ilha de patogenicidade estão presentes em amostras de R. equi virulento extraídos de animais infectados. Trabalhos recentes têm demonstrado a presença do gene vapG em todas as cepas virulentas e este gene é altamente expresso quando a bactéria reside no interior de macrófagos, tornando-o um possível alvo vacinal. Nosso grupo já possui experiência prévia no uso de linhagem de Salmonella enterica Typhimurium atenuada carreando a proteína vapA. Baseado nos resultados positivos obtidos, foi construída uma linhagem atenuada de S. enterica Typhimurium 3987 expressando a proteína vapG. A administração desta linhagem em camundongos foi capaz de induzir proteção contra R. equi virulento. Os resultados observados foram a colonização e persistência da Salmonella nos órgãos alvo, a redução da carga bacteriana de R. equi, e a indução de um perfil imune protetor semelhante ao observado em animais adultos resistentes. Observou-se o aumento da produção de IL-12p70 e IFN-, alem da presença de níveis aumentados de IL-4 e a redução dos níveis de TNF-. Observou-se também o aumento na subpopulação de células T auxiliares (CD4+) com perfil de memória, além de aumento na população de linfócitos B totais quando comparado aos grupos controles. Este conjunto de resultados indica que a imunização com Salmonella enterica Typhimurium expressando a proteína vapG gera uma resposta imune celular eficiente tornando esta linhagem uma possível candidata a vetor vacinal. Alem disso, sugere que outros antígenos do R. equi podem ser
Pulmonary infections in young foals by Rhodococcus equi result in severe pneumonia, leading to death a large number of animals every year. There is no globally accepted vaccine for the prevention of equine rhodococosis so far. To date, the only acceptable treatment is based on antibiotics, but the clinical protocols are long lasting, expensive, having side effects and favoring the emergence of drug resistant strains. Several strategies for developing a safe and effective vaccine against rhodococosis have been proposed, however, none has induced a significant protective effect. The main virulence factor of R. equi described and extensively studied is the protein vapA, although other proteins encoded by genes in pathogenicity islands are detected in samples of virulent R. equi, isolated from infected animals. Recent works have demonstrated that the vapG gene is present in all virulent strains, and that this gene is highly expressed when bacteria reside within macrophages, making it a potential vaccine target. Our group has already been working with an attenuated Salmonella enterica Typhimurium strain expressing the VapA protein. Even though our results were very promising, we decided to construct a second vaccine strain expressing the VapG protein. Based on the positive results, it was constructed an attenuated strain of S. enterica Typhimurium 3987 expressing the vapG protein. Interestingly, the VapG-expressing strain induced protection against virulent R. equi in mouse model of infection. We could observe colonization and persistence of Salmonella vaccine cells in target organs, with reduction of bacterial loads of R. equi and induction of a protective immune profile similar to that seen in resistant adult animals. We could also observe increased production of IL-12p70 and IFN- in addition to the presence of increased levels of IL-4 and reduced levels of TNF-. Moreover, we detected an increase in the subpopulation of T helper cells (CD4), with a profile of memory, as well as in the population of B lymphocytes, when compared to control groups. This set of results shows that immunization with Salmonella enterica Typhimurium expressing the VapG protein raises an efficient cellular immune response, making this strain a potential candidate for vaccine vector. Furthermore, this work suggests that other R. equi antigens may be taken into account for vaccine construction, besides the VapA protein utilized in the majority of studies.

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Schäfer, Ingmar Bastian. "Structural insights into the function of the Varp/Vamp7 complex." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609037.

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31

Rosa, Prisciane Raupp da. "Uber : de onde viemos, onde estamos e para onde vamos?" reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/168659.

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A economia compartilhada é um sistema econômico baseado no compartilhamento de bens subutilizados, de forma gratuita ou por uma taxa. Com os adventos da internet, smartphones e Gps surgiram as plataformas digitais que se baseiam nesse conceito, em que se faz emergir a necessidade da compreensão de sua relação com mercados two-side. Tal expansão atingiu diversos setores da economia, e, ainda, criou nova demanda, como a plataforma Uber, o que, no entanto, gerou distúrbios no setor de táxis, tradicionalmente regulamentado e com ineficiências mundialmente reconhecidas. Dessa forma, o objetivo dessa dissertação é entender o que são as plataformas digitais e analisar o caso especifico da empresa Uber, apresentando quais os principais fatores que permitiram que esse tipo de empresa surgisse; onde estamos, definindo o que é efetivamente a Uber e os serviços por ela prestado, abordando quais os ganhos de bem-estar proporcionados (excedente do consumidor) e problemas de regulamentação enfrentados; e para onde iremos, prospectando um caminho para a economia compartilhada a respeito das plataformas digitais em mercados two-side.
Shared economy is an economic system based on the share of underutilized goods free of charge or for a fee. With the advent of the internet, the digital platforms based on this concept have been developed, emerging the need of understanding its relationship with two-side markets. This expansion has reached various sectors of the economy, as well as created new demand, such as the Uber platform, which ,however, has generated disturbances in the taxi sector, traditionally regulated with its worldwide recognised inefficiencies. Thus, the goal of this dissertation is to understand what the digital platforms are and to analyse the specific case of Uber company, showing the main factors which allowed this type of business to arise; where we are, defining what Uber effectively is and the services provided by it, addressing the welfare gains provided (consumer surplus) and regulatory problems faced; and where we go, prospecting a path for the shared economy regarding the digital platforms in two-side markets.

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32

Abrahamsson, Christian. "Camp Vamp - Att ljussätta en dansföreställning med skiftande förutsättningar." Thesis, Malmö högskola, Fakulteten för kultur och samhälle (KS), 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-22633.

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I work on a lighting design for a dance performance to be on tour. A lighting design adapted forthree different conditions was made:- One comprehensive where I allow myself a great deal of creative freedom, adapted for thestages with major technical conditions.- One smaller version for stages with less equipment and with less time for preparation onsite.- One design for very simple conditions.I also examine the specific differences between typical theatrical lighting design and typical dancelighting design, and the best way to prepare for the hands-on stage design phase.

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33

Zhang, Ben. "The role of VAMP proteins in K+ channel regulation." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/6230/.

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SNARE (soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor) proteins drive vesicle traffic, delivering membrane and cargo to target sites within the cell and at its surface. They contribute to cell homeostasis, morphogenesis and pathogen defense. A subset of SNAREs, including the Arabidopsis SNARE SYP121, are known also to coordinate solute uptake via physical interactions with K+ channels and to moderate their gating at the plasma membrane. R-SNAREs, also known as vesicle-associated membrane proteins (VAMPs), are most commonly associated with trafficking vesicles. In guard cells of Arabidopsis, ABA-dependent stomatal closure was inhibited when the expression of VAMP71 family genes were suppressed by an antisense VAMP711 construct. Such results reveal that VAMPs play an important role in plant response to stress, especially in the regulation of stomatal closure under ABA treatment. Two R-SNAREs, VAMP721 and VAMP722, are known to assemble in SNARE core complexes with SYP121 and with its closest homolog SYP122, which raises the question whether the channel interaction might extend to the R-SNAREs leading to VAMP regulating channel gating. To answer this question, I investigated the interaction between the VAMP7 proteins with KC1 and KAT1 K+ channels by mating based Split-Ubiquitin System (mbSUS) assay, and verified these interactions by ratiometric bimolecular fluorescence complementation (rBiFC) assay. I found VAMP721 and VAMP722, but not VAMP723, interacted with the channels. The selective binding was associated with the VAMP longin domain, notably with Tyr57. What is the effect of the VAMP-K+ channel interaction on transmembrane ion transport and vesicle traffic? I found VAMP721 affected K+ channel gating and suppressed the K+ current within the physiological voltage range by electrophysiological analysis in Xenopus oocytes and in VAMP over-expression wild type Arabidopsis. The effect of VAMP721 on K+ channel regulation was opposite to the action of SYP121 on K+ channel. From localization, interaction and electrophysiological studies, I was able to show that Tyr57 is a key residue required both for VAMP-dependent gating of the K channels and for localization of the VAMPs at the plasma membrane. For vesicle traffic, I found overexpression of full length VAMP721 in Arabidopsis seedlings blocked the secretion of secYFP while coexpressing with KC1 K+ channel rescued the traffic block, demonstrating a potential action of VAMP-K+ channel interaction on secretory traffic. These results add to understanding the interaction between SNARE and K+ channels that link membrane traffic with osmotically active solute transport in the plant.

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Sadler, Jessica Betty Arnold. "Analysis of VAMP levels and function in 3T3L1 adipocytes." Thesis, University of Glasgow, 2014. http://theses.gla.ac.uk/5852/.

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A major action of the hormone insulin is to increase glucose uptake into muscle and adipose tissues. This occurs through the insulin-regulated translocation of the glucose transporter GLUT4 from intracellular deposits to the cell surface. The dysregulation of this process is a major facet of insulin-resistance and Type-2 diabetes. In the absence of insulin, GLUT4 is sequestered intracellularly in two interlinked pools. These pools are the endosomal recycling compartment and a specialised insulin-sensitive compartment. GLUT4 vesicles within the specialised insulin-sensitive compartment are termed GSVs. GSVs provide a readily available store of GLUT4 that can be rapidly mobilised to the cell surface in response to insulin stimulation. GLUT4 within the cell is continually recycled through multiple compartments, including the endosomal recycling compartment and GSVs. Perturbation of flux through any of these compartments disrupts GLUT4 traffic and thus disrupts the insulin-response. Consequently, understanding these trafficking steps is an important research goal. The formation of GSVs and their insulin-stimulated translocation to, and fusion with, the plasma membrane are examples of regulated and specific membrane trafficking events. As such these events require SNARE proteins. This study has examined the functions of each of the members of the post-Golgi vSNAREs that make up the VAMP subfamily in the trafficking of GLUT4 into the insulin-sensitive compartment, as well as examining the contribution each of these vSNAREs plays in the SNARE complex involved in the insulin-stimulated translocation of GLUT4 to the cell surface. GLUT4 traffics through the endosomal system and the trans Golgi network (TGN) en route into GSVs in a process known to involve the t-SNARE syntaxin 16. In order to dissect the roles VAMP proteins play in the intracellular recycling and trafficking of GLUT4, the interactions of VAMP proteins with syntaxin 16 and its regulatory Sec-1/Munc-18 protein, mVps45 have been examined. Further, the effects of depletion of these VAMPs in GLUT4 distribution has been examined in HeLa cells expressing HA-GLUT4-GFP as a surrogate for adipose and muscle tissue. The fusion of GLUT4-containing vesicles with the cell surface in response to insulin stimulation is a key component of the insulin-response. Although there is consensus that tSNAREs involved in this process are syntaxin 4 and SNAP23, there is discord in the literature regarding the role each vSNARE plays in this fusion event. Many studies support a role for VAMP2, however recently it has been suggested that VAMPs 3 and 8 may also be involved. The roles of these VAMPs, as well as the other post-Golgi vSNAREs expressed in adipocytes, in the fusion of GLUT4-containing vesicles with the cell surface have been examined. This has been achieved through the characterisation of the expression level, subcellular distribution of the VAMPs and determination of associations that take place between each VAMP protein and the tSNARE complex made up of syntaxin 4 and SNAP23. The results from these experiments show that despite all VAMPs being capable of forming SDS-resistant SNARE complexes with either syntaxin 4 and SNAP23 or syntaxin 16 and SNAP23, in the cellular environment only VAMP2 interacts with syntaxin 4 and only VAMP4 interacts with syntaxin 16. This, alongside the finding that each VAMP protein is expressed at varying levels and localises with different pools of GLUT4, suggests that VAMP2, not VAMPs 3 or 8, is the major vSNARE involved in the trafficking of GLUT4 to the cell surface. The finding that VAMP4 is the only VAMP that interacts with syntaxin 16, alongside the results of VAMP depletion in HeLa cells expressing HA-GLUT4-GFP, support the hypothesis that VAMP4 is involved in sorting GLUT4 out of the endosomal pool and into GSVs.

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35

Bajno, Lydia. "Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0018/MQ53328.pdf.

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36

Sword, Cameron, and Edine Bakker. "¡VAMOS! Viable Alternative Mine Operating System: A Novel Underwater Mining System." TU Bergakademie Freiberg, 2017. https://tubaf.qucosa.de/id/qucosa%3A23174.

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The 42-month ¡VAMOS! project (Viable Alternative Mine Operating System, Grant Agreement 642477, vamos-project.eu), co-funded by the European Commission’s Horizon2020 programme, will enable access to reserves of mineral deposits by developing an innovative, safe, clean, and low-visibility underwater inland mining technique. Through field-testing, ¡VAMOS! hopes to encourage investment in abandoned and prospective EU open-pit mines by providing a viable novel excavation process, ultimately aiming to reduce the EU’s reliance on imports of strategically important raw materials. The project will test the technological and economic viability of the underwater mining of inland mineral deposits which are currently economically, technologically, and environmentally unobtainable. If proven viable, ¡VAMOS! will enable access to deposits whose excavation has been historically limited by stripping ratio and hydrological and geotechnical considerations. Also, due to low noise and dust levels, and its road-transportable electric-powered system, ¡VAMOS! will be able to be applied safely in both urban-proximal and hard-to-access rural locations. ¡VAMOS! is defined by a remotely-operated underwater mining vehicle, adapted and improved from existing subsea mining technology. Operating in tandem with a remote-controlled sensory assistance-vehicle, the underwater miner will connect to a flexible riser through which mined material will be pumped from the mudline to a land-based dewatering pit via a floating mobile deployment-platform. On the deployment platform, a bypass system will be linked to production measuring equipment and a laser-induced breakdown spectroscopy system, enabling throughput monitoring and real-time grade-control. Preparatory work has been carried out to assess the regulatory compliance of the project, its likely social and environmental impact, and the steps which need to be taken to reduce and quantify these during testing. Two community stakeholder workshops held in both England and Portugal have indicated that the public is receptive to the concept. Following an official project design-freeze in October 2016, construction and integration of all components will conclude in June 2017. This will be followed by field-testing at a flooded kaolin-granite quarry in Devon, England in October 2017, with further testing planned at a flooded iron mine in Vareš, Bosnia in June 2018.

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37

Wigg, Peter. "Coupling the INDRA and VAMOS multi-detectors for symmetry energy studies." Thesis, University of Liverpool, 2015. http://livrepository.liverpool.ac.uk/2036706/.

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The study of the symmetry energy under well controlled laboratory conditions serves to provide constraints for theoretical models covering a wide range of physical phenomena, from the neutron skin thickness of 208Pb to the structure and dynamics of neutron stars. Of particular interest at the present time is the density dependence of the symmetry energy. The INDRA-VAMOS (E503) experiment intends to extract constraints on the density dependence of the symmetry energy at low densities using isotopic scaling and isospin diffusion measurements. In this report the physics motivation and experiment will be discussed in detail, as well as the calibration of the detectors and recent changes to the analysis code.

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38

Sword, Cameron, and Edine Bakker. "¡VAMOS! Viable Alternative Mine Operating System: A Novel Underwater Mining System." Technische Universitaet Bergakademie Freiberg Universitaetsbibliothek "Georgius Agricola", 2018. http://nbn-resolving.de/urn:nbn:de:bsz:105-qucosa-231090.

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The 42-month ¡VAMOS! project (Viable Alternative Mine Operating System, Grant Agreement 642477, vamos-project.eu), co-funded by the European Commission’s Horizon2020 programme, will enable access to reserves of mineral deposits by developing an innovative, safe, clean, and low-visibility underwater inland mining technique. Through field-testing, ¡VAMOS! hopes to encourage investment in abandoned and prospective EU open-pit mines by providing a viable novel excavation process, ultimately aiming to reduce the EU’s reliance on imports of strategically important raw materials. The project will test the technological and economic viability of the underwater mining of inland mineral deposits which are currently economically, technologically, and environmentally unobtainable. If proven viable, ¡VAMOS! will enable access to deposits whose excavation has been historically limited by stripping ratio and hydrological and geotechnical considerations. Also, due to low noise and dust levels, and its road-transportable electric-powered system, ¡VAMOS! will be able to be applied safely in both urban-proximal and hard-to-access rural locations. ¡VAMOS! is defined by a remotely-operated underwater mining vehicle, adapted and improved from existing subsea mining technology. Operating in tandem with a remote-controlled sensory assistance-vehicle, the underwater miner will connect to a flexible riser through which mined material will be pumped from the mudline to a land-based dewatering pit via a floating mobile deployment-platform. On the deployment platform, a bypass system will be linked to production measuring equipment and a laser-induced breakdown spectroscopy system, enabling throughput monitoring and real-time grade-control. Preparatory work has been carried out to assess the regulatory compliance of the project, its likely social and environmental impact, and the steps which need to be taken to reduce and quantify these during testing. Two community stakeholder workshops held in both England and Portugal have indicated that the public is receptive to the concept. Following an official project design-freeze in October 2016, construction and integration of all components will conclude in June 2017. This will be followed by field-testing at a flooded kaolin-granite quarry in Devon, England in October 2017, with further testing planned at a flooded iron mine in Vareš, Bosnia in June 2018.

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39

Ferreira, Josué Carlos Lourenço. "“Vamos fazer o seguinte...” : a construção apositiva na interface gramática e interação." Universidade Federal de Juiz de Fora (UFJF), 2009. https://repositorio.ufjf.br/jspui/handle/ufjf/2800.

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Este trabalho objetiva, através de uma análise de interface entre gramática e discurso, verificar como se operam as construções apositivas, cuja unidade base seja constituída do elemento linguístico o seguinte, em interações argumentativas, mais especificamente, em audiências de conciliação do PROCON/JF. Tais construções se apresentaram com notável frequência em nossos dados, revelandose uma importante estrutura de interação interpessoal modelada por características particulares. No plano interacional argumentativo, o falante se utiliza deste tipo de construção apositiva para: (i) introduzir seu ponto de vista; (ii) sustentar o ponto de vista; (iii) apresentar um acordo; e (iv) sugerir um acordo para as partes envolvidas na audiência. Já o elemento linguístico o seguinte, presente na unidade base da construção apositiva, apresenta comportamento semelhante a um marcador discursivo, desempenhando duas funções na organização do discurso: a textual (direcionada para a estrutura do texto, para o seu “amarramento”) e a interacional (relacionada aos interlocutores, aos participantes da interação). O seguinte comporta-se sintaticamente como: (i) argumento de um verbo; (ii) conjunção integrante; e (iii) conjunção explicativa. Cabe destacar que as ocorrências deste tipo de construção apositiva são todas relevantes nessa interação investigada, principalmente pela peculiaridade do elemento híbrido o seguinte que permite ao falante sinalizar ao seu interlocutor a relevância do que será dito a seguir.
This paper aims, through an analysis of the interface between grammar and discourse, see how to operate the apposition buildings, whose basic unit consists of the linguistic element o seguinte in argumentative interactions, more specifically, in conciliation hearings of PROCON / JF. Such constructions are presented with remarkable frequency in our data, revealing an important structure of interpersonal interaction modeled by particular characteristics. On the interaction of argument, the speaker uses this type of construction for patching: (i) introduce their point of view, (ii) support the view, (iii) make an agreement, and (iv) to suggest an agreement for the parties at the hearing. However the linguistic element o seguinte, that is present in the base unit of apposition buildings, behaves like a discourse marker, playing two roles in the organization of discourse: the text (directed to the structure of the text, for its "tethering") and the interactional (related to partners, the participants of the interaction). O seguinte behaves syntactically as: (i) argument of a verb, (ii) integral conjunction, and (iii) explanatory conjunction. It should be noted that occurrences of apposition constructions are all relevant in this interaction investigated, mainly by the peculiarity of the hybrid element o seguinte that allows the speaker to signal the interlocutor the relevance of what will be said below.

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Cupertino, Edson Ribeiro. "Vamos jogar RPG?: diálogos com a literatura, o leitor e a autoria." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/8/8156/tde-13022009-122722/.

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O presente trabalho é resultado da investigação envolvendo Roleplaying game, literatura e sociedade. As estruturas ficcionais e as formas do conto maravilhoso, presentes nas narrativas interativas, propiciam um resgate e reatualização da produção contemporânea na perspectiva da criação coletiva do enredo e na elaboração das personagens no fluxo de ficções. Foram utilizados como corpus as discussões nos fóruns do gênero, registros de jogadores sobre casos felizes e resultados infelizes no uso das regras da gramática da ficção. A prática que torna o leitor um cuidadoso manipulador da ficção, sujeito e criador de narrativas, pode ser observada na experiência com autoria e educação realizada numa escola pública. Finalmente, as narrativas interativas são um indicativo da necessidade de criação do ser humano ao relatar os feitos de suas personagens e os caminhos de suas histórias; denunciam não apenas as estruturas literárias e ficcionais em jogo, mas a presença de um leitor habilidoso na manipulação do universo cultural que o rodeia, cujas regras elementares são as da autoria.
The present search is resulted of the investigation involving Roleplaying loves, literature and society. The structures fictionists and the forms of the wonderful story, presents in the interactive narratives, propitiate a rescue and actualization of the contemporary production in the perspective of the collective creation of the plot and in the characters\' elaboration in the flow of fictions. It was used as a corpus of discussions in the forums of the gender, players\' registrations on happy cases and unhappy results in the use of the rules of the grammar of the fiction. The practice that turns the reader a careful manipulator of the fiction, subject and creator of narratives, it can be observed in the experience with authorship and education, accomplished at a public school. Finally, the interactive narratives are an indicative of the creation need that the human being possesses where, when telling their stories, they not just denounce the literary structures and fictionists in game, but a skilled reader\'s presence in the manipulation of the cultural universe that surrounds it, whose elementary rules are the one of the authorship.

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Fernandes, Tatiana Costa. "Vamos criar um sentimento?! Um olhar sobre a Arqueologia Pública no Brasil." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/71/71131/tde-15042008-144626/.

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Este trabalho tem por propósito principal fomentar o debate sobre as questões públicas ligadas à pesquisa científica e a profissionalização da Arqueologia no Brasil, posicionando as discussões em torno da caracterização e abrangência do campo da Arqueologia Pública. Desta forma, nos propósitos deste estudo compreende-se a Arqueologia Pública como um campo de pesquisa, debate e aplicação da Arqueologia, destinada a dialogar com a sociedade sobre as questões públicas da disciplina. O debate em torno das quatro temáticas públicas de interesse: legislação, gestão, ética e educação; almejou a definição do escopo de abrangência da Arqueologia Pública, tendo por enfoque as questões de proteção, preservação e gestão do patrimônio arqueológico, bem como, a defesa dos interesses profissionais, científicos e públicos da disciplina. Por sua vez, ao considerar o contexto específico da aplicação de uma proposta de educação não formal no sítio Topo do Guararema (Munic.Guararema, SP), foi possível vislumbrar alguns dos desmembramentos sócio-educativos da pesquisa científica, seja quando direcionada à percepção ampla das significâncias presentes no sítio ou; quando projetada a partir dos benefícios públicos e científicos por ela gerados.
This effort has as mainly purpose promote the discussion about the public questions linked to the scientific research and the profissionalization of Archaeology in Brazil, locating the discussions around the caracterization and reach of the Public Archaeology's Field. In this aspect, in the purposes of this application, is contained in the Public Archaeology as a field of reserach, debate and application of the Archaeology, destined to dialogue with the society about the public questions of the discipline. The debate around the public themes: legislation, management, ethics and education, aspiring guarantee the protection and preservation of the archaeological patrimony, as weel defend the professional interests, scientifics and publics of the discipline

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Silva, Clarissa Costa e. "Interdisciplinaridade, planejamento e as histórias de duas professoras de inglês: vamos dialogar?" Universidade Federal de Uberlândia, 2011. https://repositorio.ufu.br/handle/123456789/15397.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Mestre em Estudos Linguísticos
Este estudo narrativo investigou a experiência de construção de um planejamento de aulas de inglês, delineado a partir das vozes de alunos do primeiro ano do ensino médio de uma escola da rede pública estadual de Minas Gerais. Por um período de dois meses, a professora participante e eu construímos um planejamento de aulas de inglês que buscou se aproximar de uma perspectiva interdisciplinar de ensino e escrevemos sobre esta experiência em diários, relatos e notas de campo. A partir do estudo deste material documentário, sob a perspectiva teórico-metodológica da pesquisa narrativa, segundo Connelly e Clandinin (2000), foi possível recontar nossas histórias com planejamentos e destacar alguns aspectos emergentes de nossa experiência. Ainda, compreender como estes aspectos figuraram na imagem do planejamento elaborado e reconfiguraram alguns paradigmas de planejamento de aulas de inglês da professora participante e meus. Algumas vertentes teóricas, ligadas a conceitos e práticas curriculares e interdisciplinares, são apresentadas e discutidas de modo a problematizar o foco em questão neste estudo. Perspectivas curriculares, segundo autores como Schawb (1962), Dewey (1938; 1976), Connelly e Clandinin (1988; 1995), Mello (2005) e, ainda, perspectivas interdisciplinares, segundo Japiassú (1976), Fazenda (1978; 1997) e Freire (1987; 1996) são destaques neste trabalho. Dentre os aspectos emergentes da experiência, que deram forma à imagem do planejamento das aulas de inglês, destacaram-se: o entusiasmo, as surpresas, insatisfações, a esperança, os aspectos responsorial e relacional do planejamento, a insegurança de aproximar língua inglesa e interdisciplinaridade e os desafios de se planejar aulas. Este estudo se encontra na área de ensino e formação de professores de línguas estrangeiras da Linguística Aplicada, poderá colaborar com discussões neste campo e, possivelmente, com a prática de professores de inglês como língua estrangeira.

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Migliorança, Cássia Regina. ""Vamos tentar um tandem?": histórias de ser pesquisadora, novas tecnologias e poder." Universidade Federal de Uberlândia, 2011. https://repositorio.ufu.br/handle/123456789/15395.

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I can t say if this dissertation is the story I will tell or if this story is the dissertation I will present. Both story and dissertation are too imbricated to be distinguished. I aim at narrating and analyzing the attempt to propose an experience of learning in Tandem practice through MSN Messenger in a public school in the Triangulo Mineiro region, based on the principles of Narrative Inquiry, according to Connelly; Clandinin, 2000, 2004, 2005) and Ely (2007). I relate throughout this report mainly studies on, and experiences with, tandem practice, according to Migliorança (2007), Telles (2006), Telles; Vassallo (2005), and Brammerts and Calvert (2003); teaching and learning languages and new technologies, based on Moita Lopes (2005), Paiva (2006) and Warschauer (1996, 1997); and language and power, according to Fairclough (1989, 2009). The stories lived during the research were analyzed from the sense making principles, based on Ely, Vinz, Downing, e Anzul (2001). I could notice that the relations of power present in the research field, including assumptions I had about the use of new technologies, influenced on the research routing, resulting on the non-occurrence of the tandem practice according to the former plan and on the change of the analysis perspective after the qualification session. Despite of the (un)expected aspects what Narrative Inquiry always foresees -, technology had a meaningful role in the discovery of English learning resources by the student participants and to the beginning of a possible process of change in the teacher s practice. Furthermore, technology enabled the participants to contact students of a foreign school and moments of a cultural tandem, which seemed to have a possible impact on their learning in relation to motivation, autonomy and understanding of cultural aspects.
Não sei dizer se este trabalho é a história que vou contar, ou se esta história é o trabalho que vou apresentar. As duas coisas estão muito imbricadas para sofrerem esta distinção. Tenho como objetivo narrar e analisar a tentativa de uma experiência de aprendizagem em prática de Tandem via MSN Messenger em uma escola pública da região do Triângulo Mineiro, com base nos pressupostos da Pesquisa Narrativa, de acordo com Connelly; Clandinin, 2000, 2004, 2005) e Ely (2007). Relaciono, ao longo deste relato, principalmente, estudos sobre, e experiências com, prática de tandem, de acordo com Migliorança (2007), Brammerts e Calvert (2003), Telles; Vassallo (2005) e Telles (2006); ensino e aprendizagem de línguas e novas tecnologias, segundo Warschauer (1996, 1997), Moita Lopes (2005) e Paiva (2006); e linguagem e poder, com base em Fairclough (1989, 2009). As histórias vividas durante a pesquisa foram analisadas pela composição de sentidos, com base nos estudos realizados por Ely; Vinz; Downing; Anzul (2001). Pude perceber que as relações de poder presentes no campo de pesquisa, incluindo pressuposições que fiz sobre o uso das novas tecnologias, tiveram influência no encaminhamento da pesquisa, resultando no não acontecimento da prática de tandem conforme inicialmente planejado e na mudança de perspectiva de análise após a qualificação. Apesar dos (im)previstos que a Pesquisa Narrativa sempre prevê -, a tecnologia exerceu um papel significativo para os participantes na descoberta de recursos para aprendizagem de língua Inglesa e no início de um possível processo de mudança na prática da professora participante. Além disso, a tecnologia possibilitou aos alunos participantes o contato com alunos de uma escola estrangeira e momentos de tandem cultural, o que pareceu ter um possível impacto na aprendizagem dos alunos em relação à motivação, à autonomia e à compreensão de aspectos culturais.
Mestre em Estudos Linguísticos

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Bolaños, Natalia. "¿Vamos pa’ la Conga? Los factores detrás del conflicto social en Cajamarca." La Colmena, 2015. http://repositorio.pucp.edu.pe/index/handle/123456789/91706.

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Este trabajo trata sobre el conflicto entre la empresa minera Yanacocha y la población cajamarquina en torno al proyecto Conga. Se plantean cinco aspectos centrales al conflicto: la falta de legitimidad del Estadoy de confianza en la minera; los sentidos, significados y motivaciones divergentes entre los distintos actores; los cambios en los modos de producción que la presencia de la minera implica para la población; las distintas formas de solidaridad social e instituciones representativas y el choque entre ellas; y el desarrollo como ideología. Estos serán analizados a partir de los elementos teóricos de los clásicos de la sociología: Max Weber, Karl Marx y Émile Durkheim.

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Hastoy, Benoit. "Structure et dynamique fonctionnelle du domaine transmembranaire de la protéine SNARE VAMP2 lors de l’exocytose." Thesis, Bordeaux 1, 2011. http://www.theses.fr/2011BOR14467/document.

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Le maintien de l’homéostasie passe notamment par la sécrétion d’hormones provenant des cellules neuro-endocrines ou endocrines telles que les cellules chromaffines ou les cellules b pancréatiques. Par exemple, la régulation de la glycémie nécessite l’exocytose de l’insuline depuis les cellules b pancréatiques des îlots de Langerhans. Une famille de protéines membranaires est au cœur de la machinerie de fusion d’une vésicule avec la membrane plasmique. Ce groupe appelé, la famille des protéines SNARE est composé de trois protéines. VAMP2 est localisée à la membrane vésiculaire alors que syntaxine 1A et SNAP25 sont localisées à la membrane plasmique. Syntaxine 1A et VAMP2 ont un domaine transmembranaire alors que SNAP25 est reliée à la membrane par prénylation de résidus cystéine. Cette famille forme le complexe cytosolique SNARE décrit comme essentiel à l’exocytose. La structure et la fonction du complexe cytosolique ont été étudiées en profondeur et ont mené au modèle du « zipper ». Celui-ci décrit un enroulement progressif des domaines cytosoliques SNARE permettant l’apposition des membranes puis la fusion. Le rôle des domaines transmembranaires reste encore peu décrit. Pourtant, leur étude est nécessaire afin d’établir un modèle complet de la fusion membranaire par les protéines SNARE. Nous avons donc mené une étude alliant une analyse structurale dynamique à une analyse biologique pour déterminer l’importance du domaine transmembranaire de VAMP2 dans la sécrétion. L’analyse biologique représente donc le centre de ma thèse. Le système biologique utilisé est basé sur l’extinction de l’expression de la protéine VAMP2 endogène et l’expression concomitante d’une protéine VAMP2 mutée dans son domaine transmembranaire. Deux lignées cellulaires considérées comme des modèles dans l’étude de la sécrétion hormonale et du trafic vésiculaire ont servi de support à notre étude. Par des approches de microscopies (confocal, TIRF) et d’analyses biochimiques, nous avons observé les conséquences fonctionnelles des mutations ponctuelles, établis par mutagénèse dirigée, sur le trafic vésiculaire et sur la capacité des cellules à sécréter.Les mutations induites présentent différents effets cellulaires. Certaines bloquent la sortie de VAMP2 du réseau golgien alors que d’autres ont un effet important sur la sécrétion hormonale et plus précisément sur l’exocytose. Les études structurales ont permis de corréler ces effets avec une diminution de la flexibilité structurale dans le cas de la diminution de l’exocytose, ou avec une restriction à la conformation hélice alpha dans le cas du sorting. Ce projet pluridisciplinaire a pu mettre en avant le rôle biologique du domaine transmembranaire de VAMP2 au cours de l’exocytose probablement soutenue par la dynamique conformationelle unique observée par le versant structural du projet
The hormonal secretion plays a key role in the maintenance of homeostasis. For example, the maintenance of normoglycaemia requires insulin exocytosis from the pancreatic beta cells. The SNARE membrane family protein has been described as the core machinery of fusion between the vesicle containing hormones and the plasma membrane. This family consists of 3 different membrane proteins that are essential during exocytosis. VAMP2 is localized on the vesicle and Syntaxin 1A - on the plasma membrane. They both are transmembrane protein whereas SNAP25 is linked to the plasma membrane by palmitoylation. The SNAREs appear to be essential as they form the cytosolic SNARE complex to dock the vesicle to the plasma membrane. Even though the role of this cytosolic domain has been studied in depth, much less is known on the role of their transmembrane domain during the fusion. Their study remains necessary to establish a complete model of membrane fusion mediated by the SNARE proteins.Here, we have studied the behavior and the role of the SNARE transmembrane domain during exocytosis. In a multidisciplinary project, we have combined a structural approach with a biological study to evaluate the role of this domain. Using mutagenesis in the transmembrane domain of VAMP2 and a cellular system with a clean background, we have assessed the effect of mutations on the secretion and exocytosis in two different cell lines (INS1E and PC12). The biological system is based on the silencing of endogenous VAMP2 and reconstitution of the expression of VAMP2 wt or mutated in the transmembrane domain. Using biochemistry assay and TIRF microscopy we have shown that mutations in this domain can lead to a missorting of the Golgi apparatus or a reduction of the stimulated secretion and exocytosis. This effect can be correlated to a modification of the structural dynamics of this domain.The obtained results clearly demonstrate the role of the transmembrane domain of VAMP2 during exocytosis probably sustained by its unique structural dynamics observed by physico-chemistry

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Poirier, Laurie Ann. "Chronic ethanol feeding decreases insulin-stimulated Glut4 and VAMP2 translocation in rat adipocytes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0028/MQ31858.pdf.

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Storti, Felipe Chagas 1982. "Estudo experimental da eficiência de separação da câmara de expansão do separador VASPS." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/265926.

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Orientador: José Ricardo Pelaquim Mendes
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecânica e Instituto de Geociências
Made available in DSpace on 2018-08-24T13:52:12Z (GMT). No. of bitstreams: 1 Storti_FelipeChagas_D.pdf: 7344405 bytes, checksum: c9c2f6d0dd3dea372babaa14915d4b0d (MD5) Previous issue date: 2013
Resumo: O sistema de separação submarina VASPS (Vertical Annular Separation and Pumping System), que se utiliza tanto do efeito gravitacional quanto do centrífugo é composta por Câmara de Expansão, Helicóide de Separação e Piscina. Com o objetivo de se estudar um separador compacto e de menor custo, foi construído um protótipo da Câmara de Expansão em acrílico. Foram realizados testes de eficiência de separação do protótipo utilizando a mistura ar-água e a mistura ar-glicerina com diferentes viscosidades. Para realizar os testes de eficiência foi construído um circuito fechado todo equipado e instrumentado. Nestes testes de eficiência variou-se o diâmetro de bocal de entrada do separador e o nível de líquido na parte inferior do separador. Foi observado durante os testes experimentais a formação de um vórtice, principalmente para níveis mais baixos de líquido, diminuindo a eficiência do separador. Para solucionar esse problema foi desenvolvido e fabricado um dispositivo chamado Redutor de Vórtice, melhorando a eficiência do protótipo para certas condições de operação. Os resultados mostraram que a eficiência diminui para fluidos mais viscosos e também observou-se que o padrão de escoamento no bocal de entrada do separador teve influencia na eficiência. Resultados do ângulo de inclinação e altura de elevação do filme de líquido na parede do separador também foram obtidos
Abstract: The VASPS (Vertical Annular Separation and Pumping System) is a subsea separation system, which utilizes both the gravitational effect as the centrifugal and is composed by Expansion Chamber, Helicoide Separation and Pool. In order, to study a compact separator and with lower cost, we built a prototype of Expansion Chamber in acrylic. Separation efficiency of the prototype using the air-water mixture and air-glycerin mixture with different viscosities tests were performed. To perform efficiency tests was built a closed circuit all equipped and instrumented. In these tests of efficiency ranged the diameter of the separator inlet nozzle and the liquid level at the bottom of the separator. The formation of a vortex, especially for lower levels of liquid was observed during the experimental tests, decreasing the efficiency of the separator. To solve this problem was developed and manufactured a device called Vortex Reducer, improving the efficiency of the prototype for certain operating conditions. The results showed that the efficiency decreases for more viscous fluids and also observed that the flow pattern at the nozzle inlet separator had an influence in the efficiency. Results of tilt angle and lift height of the liquid film on the wall of the separator were also obtained
Doutorado
Explotação
Doutor em Ciências e Engenharia de Petróleo

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Rigo, Kate Fabiani. "Vamos começar pelo fim?: a pedagogia cemiterial como projeto educativo no espaço escolar." Faculdades EST, 2015. http://tede.est.edu.br/tede/tde_busca/arquivo.php?codArquivo=662.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
A tese tem o objetivo de apresentar a Pedagogia Cemiterial como proposta de ação a ser desenvolvida no ensino básico. A metodologia escolhida fundamenta-se na metanálise de dados do Censo de 2010, referentes ao perfil religioso dos/das adolescentes brasileiros e na pesquisa etnográfica virtual, através do monitoramento de Páginas Comunitárias do Facebook ligadas ao incentivo e extinção do comportamento autolesivo e suicidário entre adolescentes brasileiros (as) de 13-17 anos de idade. A estrutura foi dividida em quatro capítulos distintos. O primeiro apresenta o indivíduo, na fase da adolescência, a partir do modelo bioecológico de Bronfenbrenner e como este se insere nos estágios da fé e o Deus Google. O segundo verifica a percepção de morte e morrer do/da adolescente virtualizado (a), bem como sua interação com as Páginas Comunitárias do Facebook associadas ao incentivo e extinção do comportamento autolesivo. O terceiro analisa a importância do Ensino Religioso como componente curricular compatível ao desenvolvimento do tema da morte e do morrer no espaço escolar através da Pedagogia Cemiterial. Por fim, o último capítulo propõe um modelo de ação para a aplicação da Pedagogia Cemiterial com adolescentes no espaço escolar.
The goal of this dissertation is to present the Cemeterial Pedagogy as a proposal for action to be developed in primary education. The methodology chosen is founded on the meta-analysis of data from the 2010 Census, regarding the religious profile of Brazilian adolescents and on the virtual ethnographic research, through the monitoring of Community Facebook pages connected to the encouragement and extinction of self-injurious and suicidal behavior among Brazilian adolescents ages 13 to 17. The structure was divided into four distinct chapters. The first presents the individual, in the adolescent phase, based on the bio-ecological model of Bronfenbrenner and how this adolescent inserts him/herself in the faith stages and the Google God. The second verifies the perception of death and dying of the virtualized adolescent as well as his or her interaction with Community Facebook pages associated to the encouragement and extinction of self-injurious behavior. The third analyzes the importance of Religious Education as a curricular component that is compatible to the development of the theme of death and dying in the school space through the Cemeterial Pedagogy. Finally, the last chapter proposes a model of action for the application of the Cemeterial Pedagogy with adolescents in the school space.

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Russo, Maria José de Oliveira. "ALUNOS COM DEFICIÊNCIA NO ENSINO REGULAR: E AGORA PROFESSOR, VAMOS FALAR DE INCLUSÃO?" Universidade Metodista de São Paulo, 2007. http://tede.metodista.br/jspui/handle/tede/1073.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The inclusion of disabled students into the current teaching in Brazil is a movement that has been rising lately. Although advances have been noticed, because it is a process which needs conceptual, legal, and practice changes, brings itself some doubts, because of existent contradictions between the discourses that proclaim the inclusion and the Brazilian educational reality. Under the vision of the teachers, in the context of the inclusive school, there are several difficulties that the teachers are facing, in practice, with these students. Considering this, despite of the existing production in this field of research, it is necessary to go deeper into the research of this theme from the point of view of the teacher. So, this research investigates the voice and the optics of the teachers of regular classes that have students in situation of inclusion, more specifically the disable ones and deals with different aspects of the inclusion in the school quotidian and the support to the teacher as one of the necessary actions for the inclusive pedagogic practice highlighting the theorists that discourse the inclusive process. The study, of a qualitative character, was realized in four municipal Elementary schools of São Bernardo do Campo with the participation of nine teachers, four principals and four families of disabled children. Considering the analyze of the observed aspects, we can say that the teachers are searching for a re-signification of their practices, however, they need a constant support to deal with the diversity of these students.(AU)
A inclusão de alunos com deficiência em ensino regular no Brasil é um movimento que vem denotando constante crescimento nos últimos anos. Embora muitos avanços sejam observados, por ser um processo que suscita mudanças conceituais, legais e de prática, traz consigo dúvidas, decorrentes das contradições existentes entre os discursos que proclamam a inclusão e a realidade educacional brasileira. Sob o prisma da docência, no contexto da escola inclusiva, são inúmeras as dificuldades que os professores vêm enfrentando na prática com esse alunado. Considerando essa problemática, apesar da produção já existente, faz-se necessário o aprofundamento de estudos deste tema do ponto de vista do professor. Portanto, esta pesquisa investiga a voz e a ótica dos professores das classes regulares que possuem alunos em situação de inclusão, mais especificamente os que têm deficiência, e aborda os diferentes aspectos da inclusão no cotidiano escolar e o apoio ao professor como uma das ações necessárias para a prática pedagógica inclusiva à luz de teóricos que discutem o processo inclusivo. O estudo, de caráter qualitativo, foi realizado em quatro escolas do Ensino Fundamental (séries iniciais) da rede pública municipal de São Bernardo do Campo, e contou com a participação de nove professores, quatro diretores e quatro famílias de alunos com deficiência. Considerando a análise dos aspectos observados, pode-se inferir que os professores têm buscado a ressignificação de suas práticas, mas desvelam a necessidade de um apoio constante, que lhes dê sustentação para o atendimento à diversidade dos seus alunos.(AU)

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Pandeirada, Cátia Sofia Martins. "Abordagem didática CTS no pré-escolar : o peixe é fish, vamos à pesca!" Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/16380.

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Mestrado em Educação Pré-Escolar e Ensino do 1º Ciclo do Ensino Básico
O presente relatório final de estágio pretende apresentar o projeto de intervenção-investigação desenvolvido numa perspetiva da educação para a sustentabilidade. O projeto de intervenção-investigação pretendia trabalhar a temática do peixe e os benefícios do consumo do mesmo para as crianças, constituindo-se por uma sequência didática que envolveu o trabalho sobre questões relacionadas com diferentes espécies de peixe que existem. O projeto de intervenção-investigação implementado teve como principais objetivos: i) avaliar o impacte da sequência didática nas aprendizagens das crianças ao nível dos conhecimentos, capacidades e atitudes e valores e ii) avaliar o impacte da sequência didática no desenvolvimento profissional da professora investigadora que a concebeu, implementou e avaliou. O projeto de intervenção-investigação teve como suporte os seguintes pilares teóricos: i)Educação para o Desenvolvimento Sustentável; ii) Literacia Científica; iii) Educação em Ciências nos primeiros anos; iv) Orientação Ciência, Tecnologia e Sociedade e v) IBSE/EPP. O mesmo projeto teve caraterísticas de investigação-ação, em que se utilizaram como técnicas de recolha de dados a observação, o inquérito por entrevista e a compilação documental. Na análise dos dados recorreu-se à análise de conteúdo com suporte ao software WebQDA. O projeto contribuiu para o desenvolvimento de aprendizagens das crianças ao nível dos conhecimentos, como “Conhece algumas espécies de peixe”; capacidades como “Observa”; e atitudes e valores como “Revela gosto pelo consumo de peixe”; tal como para a promoção do desenvolvimento profissional da professora investigadora, o que se evidencia nos resultados.
This final stage of report aims to present the project of intervention-research developed a perspective of education for sustainability. The project intervention-research intended to work the theme of fish and the benefits of its consumption for children, being by a didactic sequence that involved working on issues related to different species of fish that exist. The implemented research-intervention project was primarily to: i) assess the impact of the didactic sequence in the learning of children in terms of knowledge, skills and attitudes and values and ii) assess the impact of the didactic sequence in the professional development of researcher teacher who She conceived, implemented and evaluated. The action-research project was supported by the following theoretical pillars: i) Education for Sustainable Development; ii) Scientific Literacy; iii) Science Education in the early years; iv) Guidance Science, Technology and Society v) IBSE / EPP. The same project was research-action features, which were used as techniques for data collection observation, interview survey and documentary compilation. In analyzing the data resorted to content analysis with support WebQDA software. The project contributed to the development of learning of children at the level of knowledge as "Know some species of fish"; capabilities as "Notes"; and attitudes and values as "reveals taste for fish consumption"; as to promote the professional development of teacher researcher, as evidenced in the results. The results show that the project was constituted as a stimulus to science education from the early years, revealing the possibility and similar oriented project development relevance of different themes for the formation of more responsible and conscious citizens in promoting development sustainable.

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Dissertations / Theses on the topic 'Vamps'

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