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Journal articles on the topic 'Vancomycin sensitive enterococci'

Author: Grafiati

Published: 9 August 2021

Last updated: 29 July 2025

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1

Szabó, Marcell, Anna Bozó, Sándor Soós, Katalin Darvas, László Harsányi, and Ákos Csomós. "Vancomycinrezisztens Enterococcusok megjelenése sebészeti intenzív osztályon." Orvosi Hetilap 156, no. 19 (2015): 779–84. http://dx.doi.org/10.1556/650.2015.30135.

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Introduction: Enterococci have increasing importance in intensive care units, and vancomycin-resistant strains express a new challenge. Aim: The aim of the authors was to present their findings obtained from the first vancomycin-resistant enterococci outbreak occurred in 2013 at the Intensive Care Unit of the 1st Department of Surgery, Semmelweis University. Method: This was a case-control study of patients who had Enterococci species isolated from their microbiological samples between January 1 and June 30, 2013. Changes of Enterococcal incidence and consequences of vancomycin-resistance in p

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Yazgi, H., M. Ertek, S. Erol, and A. Ayyildiz. "A Comparison of High-Level Aminoglycoside Resistance in Vancomycin-Sensitive and Vancomycin-Resistant Enterococcus Species." Journal of International Medical Research 30, no. 5 (2002): 529–34. http://dx.doi.org/10.1177/147323000203000510.

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The aim of this study was to investigate whether there was a significant difference in high-level aminoglycoside resistance (HLAR) between vancomycin-sensitive enterococci (VSE) and vancomycin-resistant enterococci (VRE). Vancomycin resistance was determined in 116 Enterococcus isolates using brain-heart infusion agar containing 6 μg/ml vancomycin. HLAR was determined by both standard agar screening and disk diffusion methods. Streptomycin and gentamicin were used as predictors of HLAR. Vancomycin resistance and HLAR were found in 17 (14.7%) and 41 (35.3%) of the Enterococcus strains, respecti

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Tamanna, Shakila, Lovely Barai, AA Ahmed, and J. Ashraful Haq. "High level gentamicin resistance and susceptibility to Vancomycin in enterococci in a tertiary care hospital of Dhaka City." Ibrahim Medical College Journal 7, no. 2 (2014): 28–31. http://dx.doi.org/10.3329/imcj.v7i2.20103.

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Vancomycin and high level gentamicin resistant enterococci detection is important for effective treatment and control of nosocomial infection. The present study was undertaken to determine the species distribution of Enterococcus and the rate of vancomycin and high level gentamicin resistant enterococci (HLGRE) in clinical samples in a tertiary care hospital of Dhaka city. Enterococci were identified to species level by standard biochemical and serological methods. Their susceptibilities to antibiotics were determined by disc diffusion method according to CLSI guideline. Minimum inhibitory con

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Zerbato, Verena, Riccardo Pol, Gianfranco Sanson, et al. "Risk Factors for 30-Day Mortality in Nosocomial Enterococcal Bloodstream Infections." Antibiotics 13, no. 7 (2024): 601. http://dx.doi.org/10.3390/antibiotics13070601.

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Enterococci commonly cause nosocomial bloodstream infections (BSIs), and the global incidence of vancomycin-resistant enterococci (VRE) BSIs is rising. This study aimed to assess the risk factors for enterococcal BSIs and 30-day mortality, stratified by Enterococcus species, vancomycin resistance, and treatment appropriateness. We conducted a retrospective cohort study (2014–2021) including all hospitalized adult patients with at least one blood culture positive for Enterococcus faecalis or Enterococcus faecium. We included 584 patients with enterococcal BSI: 93 were attributed to vancomycin-r

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Huebner, Johannes, Alexander Quaas, Wolfgang A. Krueger, Donald A. Goldmann, and Gerald B. Pier. "Prophylactic and Therapeutic Efficacy of Antibodies to a Capsular Polysaccharide Shared among Vancomycin-Sensitive and -Resistant Enterococci." Infection and Immunity 68, no. 8 (2000): 4631–36. http://dx.doi.org/10.1128/iai.68.8.4631-4636.2000.

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ABSTRACT Enterococci are important nosocomial pathogens that are increasingly difficult to treat due to intrinsic and acquired resistance to antibiotics, including vancomycin. A recently described capsular polysaccharide (CP) isolated from Enterococcus faecalis 12030 was used to evaluate the potential efficacy of active or passive immunotherapy regimens as adjunctive treatments. Evaluation of protective efficacy was carried out in immunocompetent mice challenged intravenously (i.v.) with live enterococci. In nonimmune mice, i.v. inoculations resulted in high levels of bacteria in kidneys, sple

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Surbhi, Suneja, Lalwani Jaya, Shrivastava Rakesh, and Chaurasia Deepti. "Characterization and detection of Linezolid resistance in clinical isolates of Enterococci in Tertiary care Hospital of Central India." International Journal of Pharmaceutical and Clinical Research 15, no. 8 (2023): 1549–54. https://doi.org/10.5281/zenodo.11528544.

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<strong>Introduction:</strong> The prevalence of antibiotic resistance within the Enterococcus species is on the rise. <strong>Aim:</strong> This study aimed to ascertain the occurrence of linezolid-resistance amongst the Enterococcal bacterial specimens isolated from patients. <strong>Materials and Methods:</strong> This was a single centre, hospital; laboratory based clinical cross-sectional study conducted over a period of 18 months. The Enterococcus bacteria isolated and identified from diverse samples specimen were subjected to culture and antibiotic sensitivity t

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7

Salu Kumari Singh, Vasundhara Sharma, and Umar Farooq. "Detection of Vancomycin-Resistant Enterococcus in Clinical Settings in a Tertiary Care Hospital." Indian Journal of Public Health Research & Development 16, no. 3 (2025): 282–89. https://doi.org/10.37506/fzqeaa39.

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Background: Enterococcus is a nosocomial infection that has become more prevalent due to its innate resistance to conventional antibiotics and recent acquisition of resistance to other current therapeutic alternatives. Aim- Detection of vancomycin-resistant Enterococcus in clinical settings in a tertiary care hospital. Objective- The main objective of this study is to isolate and identify the antimicrobial susceptibility test pattern of the Enterococci from different samples and detect Vancomycin-resistant Enterococcus among the isolated Enterococci strains. Material & Method: This study w

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8

Tapalsky, D. V., and K. M. Kosenkova. "Antibacterial Activity of Hypogymnia Physodes Extract and its Combinations with Antibiotics Against Enterococci." Health and Ecology Issues, no. 4 (December 28, 2018): 52–56. http://dx.doi.org/10.51523/2708-6011.2018-15-4-10.

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Objective : to study antibacterial properties of Hypogymnia physodes extract and its combinations with antibiotics against vancomycin-sensitive and vancomycin-resistant strains of enterococci. Material and methods. Minimum inhibitory concentrations (MICs) of the acetone extract of H. physodes for reference and clinical isolates of Enterococcus faecalis were determined by the serial broth dilution method. Evaluation of efficiency of the combined effect of the H. physodes extract in combination with antibiotics was carried out using the modified disc-diffusion method and «chessboard» method. Res

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Kolář, M., R. Pantůček, J. Bardoň, et al. "Occurrence of antibiotic-resistant bacterial strains isolated in poultry." Veterinární Medicína 47, No. 2 - 3 (2012): 52–59. http://dx.doi.org/10.17221/5803-vetmed.

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The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999&ndash;2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent gramnegatives and grampositives, the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased fre

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Lee, Sung Young, Ji-Hyun Nam, Jung Wook Kim, Soo Hyun Kim, and Jung Sik Yoo. "Prevalence of Vancomycin-Variable Enterococci from the Bloodstream in the Korea Global Antibiotic Resistance Surveillance System, 2017–2022." Antibiotics 13, no. 12 (2024): 1210. https://doi.org/10.3390/antibiotics13121210.

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Vancomycin-variable enterococci (VVE), though genetically containing van genes, are phenotypically sensitive to vancomycin. If VVE is undetected or does not grow on the vancomycin-resistant enterococci (VRE) selection medium, or both, it can acquire resistance upon exposure to vancomycin. This characteristic is clinically important for the treatment and prevention of VRE. This study aims to analyze the prevalence and characteristics of VVE in Korea through the Global Antibiotic Resistance Surveillance System (Kor-GLASS) and emphasize the importance of VVE. A total of 3342 enterococcal bloodstr

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Zerrouki, Hanane, Sid-Ahmed Rebiahi, Linda Hadjadj, Jean-Marc Rolain, and Seydina M. Diene. "Real-Time PCR Assay for Rapid and Simultaneous Detection of vanA and vanB Genes in Clinical Strains." Diagnostics 11, no. 11 (2021): 2081. http://dx.doi.org/10.3390/diagnostics11112081.

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Here, we develop a robust and sensitive real-time PCR assay which allows the simultaneous detection of vanA and vanB genes using common primers. The system was designed using the Primer3 online software. The specificity of primers and probes was first checked by in silico PCR and by BlastN analysis. The genomic DNA of 255 bacterial isolates, including Enterococcus spp., Gram-negative, and Gram-positive strains, as well as a collection of 50 stool and 50 rectal swab samples, were tested to evaluate the specificity of the new real-time PCR (RT-PCR) system. The results of the designed RT-PCR were

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Singh, Bhoj Raj. "Prevalence of vancomycin resistance and multiple drug resistance in enterococci in equids in North India." Journal of Infection in Developing Countries 3, no. 07 (2009): 498–503. http://dx.doi.org/10.3855/jidc.467.

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Introduction: Vancomycin resistant and multi-drug-resistant enterococci are the major emerging pathogens in surgical, neonatal, and tertiary care units. Methodology: In this study, 267 enterococci from different clinical and non-clinical samples of equine origin were tested for their antimicrobial drug sensitivity against 19 antimicrobials using disc diffusion method. Results: A total of 80.2% enterococci tested were resistant to vancomycin and 99.6% to multiple-drugs. There was a significant association between haemolytic potential and vancomycin resistance (χ2, 0.00). Enterococci isolates fr

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Abro, Hakim Ali, Sheeraz Ali, Sultan Ahmed Chandio, Jamil Muqtadir, Mumtaz Ali Chutto, and Chandur Lal. "Common Pathogen and their Drug Sensitivity Pattern in Urinary Tract Infection at Chandka Medical College Hospital Larkana." Pakistan Journal of Medical and Health Sciences 15, no. 7 (2021): 1868–72. http://dx.doi.org/10.53350/pjmhs211571868.

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Objective: To study the pattern of existing common pathogen of Urinary Tract Infection and their drug sensitivity pattern. Methods: It was a cross sectional study held in Medical Unit-I, CMC Teaching Hospital, Shaheed Mohtarma Benazir Bhutto Medical University(SMBBMU), Larkana with collaboration of Pathology Laboratory CMC Larkana, Aga Khan University Hospital Lab, Rahila Research Lab and Chughtai Lab. All the participants meeting inclusion criteria were enrolled. The reports of Urine culture were collected from above mentioned laboratories which include bio data, causative organism, their cou

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SujonAli, Md, Md AshiqurRahman, Sadia Islam, et al. "Prevalence and Antibiotic Susceptibility Pattern of Enterococcus Spp. Isolated from Urine Samples." Molecular Mechanism Research 1, no. 1 (2023): 134. http://dx.doi.org/10.59429/mmr.v1i1.134.

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Enterococci are the most common cause of healthcare-associated urinary tract infections. Enterococcus spp. were isolated from each of Urine and differences for Enterococcus contamination in urine were determined. Because of its unimportant commensals, Enterococci have developed into diverse, deadly diseases due to the routinely prescribed antibiotics for the treatment of nosocomial UTIs are less effective, the developing antibiotic resistance is concerning. This study aimed to determine the prevalence and antibiotic susceptibility patterns of different enterococcal species isolated from urine

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15

Reynolds, P. E., H. A. Snaith, A. J. Maguire, S. Dutka-Malen, and P. Courvalin. "Analysis of peptidoglycan precursors in vancomycin-resistant Enterococcus gallinarum BM4174." Biochemical Journal 301, no. 1 (1994): 5–8. http://dx.doi.org/10.1042/bj3010005.

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Vancomycin resistance in enterococci is an increasing clinical problem, and several phenotypes have been identified. We demonstrate here that the resistance mechanism in the constitutively vancomycin-resistant Enterococcus gallinarum BM4174 involves an altered pathway of peptidoglycan synthesis and hydrolysis of the normal precursors in the vancomycin-sensitive pathway. A ligase encoded by the vanC gene catalyses synthesis of D-Ala-D-Ser and substitutes this dipeptide for D-Ala-D-Ala in peptidoglycan precursors. It is presumed that this substitution lowers the affinity of vancomycin for its ta

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Naruka, Harshad Singh, Anita E. Chand, Pradhuman Singh Chauhan, and Danish Mukhtar. "Detection of various virulence factors in high level aminoglycoside resistance and vancomycin resistant enterococci isolates of uropathogenic Enterococci." International Journal of Research in Medical Sciences 7, no. 5 (2019): 1740. http://dx.doi.org/10.18203/2320-6012.ijrms20191668.

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Background: Enterococci are common commensal organism of enteric tract and act as opportunistic pathogen and may cause infection in community as well as in hospitalised individuals. In present study association of several types of virulence factors like haemolysin, gelatinase and biofilm formation have been studied among HLAR and Vancomycin resistant Enterococci (VRE) isolates of enterococci among UTI patients.Methods: The samples were collected from all hospitalized and OPD patients of MBS Hospital, JK Lone Hospital and NMC Hospital. Government Medical College, Kota, Rajasthan, India. A total

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Paul, Manisha, Prem Singh Nirwan, and Preeti Srivastava. "Detection of high-level aminoglycoside resistance by disc diffusion and e-test amongst the enterococcus species isolated from various clinical samples in a tertiary care hospital." International Journal of Research in Medical Sciences 7, no. 9 (2019): 3527. http://dx.doi.org/10.18203/2320-6012.ijrms20193941.

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Background: The emergence of Enterococcus species in causing nosocomial infections poses a therapeutic challenge to clinicians. Enterococci are intrinsically resistance to multiple antibiotics. Acquired resistance to commonly used antibiotics like Ampicillin, Vancomycin and Aminoglycosides have made the situation worse and difficult to treat serious Enterococcal infections. The present study aimed at detection of high-level aminoglycoside resistance by disc diffusion and E-test amongst the Enterococcus species isolated from various clinical samples in a tertiary care hospital.Methods: A total

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Al Bshabshe, Ali, Abdullah Algarni, Yahya Shabi, et al. "Characterization and Antimicrobial Susceptibility Patterns of Enterococcus Species Isolated from Nosocomial Infections in a Saudi Tertiary Care Hospital over a Ten-Year Period (2012–2021)." Diagnostics 14, no. 11 (2024): 1190. http://dx.doi.org/10.3390/diagnostics14111190.

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Introduction: The Enterococcus genus is a common cause of nosocomial infections, with vancomycin-resistant enterococci (VRE) posing a significant treatment challenge. Method: This retrospective study, spanning ten years (2012 to 2021), analyzes antimicrobial susceptibility patterns of Enterococcus species from clinical samples in a Saudi Arabian tertiary care hospital. Result: A total of 1034 Enterococcus isolates were collected, 729 from general wards and 305 from intensive care unit (ICU) patients. VRE accounted for 15.9% of isolates. E. faecalis was the most common species (54.3% of isolate

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Jain, Swati, Vikas Jain, and Shweta Jain. "Identification of vancomycin resistance in enterococcus & determination of minimal inhibitory concentration of vancomycin by E-test." Panacea Journal of Medical Sciences 14, no. 2 (2024): 356–59. http://dx.doi.org/10.18231/j.pjms.2024.063.

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Infections due to Enterococcal species is 2 prevalent cause of Nosocomial Infections in health care settings. They are crucial because to their inherent resistance to penicillins, cephalosporins, and low level aminoglycosides & their tendency to develop higher level of aminoglycoside and vancomycin resistance, which leaves few therapeutic alternatives for therapy. Isolation and identification of Enterococci with their Antibiogram & determination of Minimal Inhibitory Concentration (MIC) of Vancomycin by E-test. The antibiogram generated by Kirby Baur disc diffusion method and standard

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Roghmann, Mary-Claire, Beulah E. Perdue, and Louis Polish. "Vancomycin Use in a Hospital With Vancomycin Restriction." Infection Control & Hospital Epidemiology 20, no. 01 (1999): 60–63. http://dx.doi.org/10.1086/501548.

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AbstractWe evaluated vancomycin use in a hospital with endemic vancomycin-resistant enterococci and a vancomycin restriction program. Only 68% of vancomycin was prescribed appropriately. Inappropriate use was due primarily to empirical therapy. In the patients with a microbiological diagnosis following empirical therapy, 83% (25/30) had infections due to bacteria sensitive to an appropriate antibiotic other than vancomycin. However, only 60% (15/25) of these patients had their vancomycin orders changed.

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Jaiswal, Shweta, Amit Singh, Rajesh K. Verma, Dharmendra P. Singh, and Sunita Kumari. "Characterization, speciation and antimicrobial resistance pattern of Enterococcus species isolated from clinical specimens at a rural tertiary care hospital." International Journal of Research in Medical Sciences 5, no. 8 (2017): 3484. http://dx.doi.org/10.18203/2320-6012.ijrms20173546.

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Background: Enterococci have attracted much attention in recent years due to their increased recognition as a cause of nosocomial infection. They exhibit vancomycin resistance with increasing frequency so that determination of antimicrobial susceptibility pattern is necessary. Aim of present study is to determine the prevalence and susceptibility pattern of Enterococci in rural tertiary care hospital.Methods: Total of 100 Enterococcus isolated from various clinical samples were identified and speciated by Vitek®2 compact system (BIOMERIEUX). Antibiotic susceptibility was determined by modified

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Biswas, PriyankaPaul, Sangeeta Dey, Aninda Sen, and Luna Adhikari. "Molecular characterization of virulence genes in vancomycin-resistant and vancomycin-sensitive enterococci." Journal of Global Infectious Diseases 8, no. 1 (2016): 16. http://dx.doi.org/10.4103/0974-777x.176141.

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Ghimire, Sudeep, Hom Bahadur Basnet, Lok Raj Joshi, and Maheshwar Sapkota. "Prevalence of Vancomycin Resistant Enterococci Species in Minced Buffalo Meat of Chitwan, Nepal." International Journal of Applied Sciences and Biotechnology 2, no. 4 (2014): 409–12. http://dx.doi.org/10.3126/ijasbt.v2i4.11123.

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Vancomycin resistant Enterococci is becoming one of the concerns of public health in recent times. VRE involve those strains of Enterococci that are resistant to the vancomycin which is considered as last drug of choice for the Gram positive bacterial infections. Studies show enterococci to be one of the major food borne diseases and medical consequences of its occurrence in humans. Thus, a cross sectional studywas carried out to determine the prevalence of VRE in buffalo meat in Chitwan. A total of 63 retail minced meat samples were collected from different meat shops. These samples were dilu

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Khisa, Suman, Arif Ahmed Khan, Jamal Pasha Chowdhury, and Wasila Rahman. "Prevalence and Antibiotic Sensitivity Pattern of Enterococci Causing Urinary Tract Infection in a Tertiary Care Hospital of Bangladesh." Journal of Armed Forces Medical College, Bangladesh 20, no. 2 (2025): 27–29. https://doi.org/10.3329/jafmc.v20i2.80407.

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Background: Enterococcus has emerged as one of the important causative agents of urinary tract infection (UTI) in recent years. Their resistance to many antimicrobials has increased in last few decades. Objective: To find out the appropriate antibiotics to treat the patient caused by different species of enterococci. Methods: This cross-sectional observational study was carried out in Armed Forces Institute of Pathology (AFIP) and Combined Military Hospital (CMH), Dhaka Cantonment, Dhaka during the period of December 2022 to May 2023. During this period, a total of 118 enterococci were isolate

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Soujanya, Betu Rama, and Banashankari G S. "Utility of Chromogenic Medium in Characterization of Enterococci in Urinary Tract Infection and Phenotypic Detection of Their Virulence Factors." International Journal of Health Sciences and Research 11, no. 5 (2021): 278–83. http://dx.doi.org/10.52403/ijhsr.20210544.

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Introduction: Enterococci from being intestinal commensals have evolved in becoming pathogens and are associated with significant morbidity and mortality Aims & Objectives: This study was done to speciate the uropathogenic Enterococci using the chromogenic medium and to determine the antibiogram also to detect virulence factors phenotypically. Materials and methods: The study included a total of 30 uropathogenic Enterococci isolated over 6 months. Speciation was done using HiCrome Enterococcus faecium agar base. Antibiotic sensitivity was done by the Kirby Bauer Disc Diffusion method as pe

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Yurdakul, N. E., Z. Erginkaya, and E. Ünal. " Antibiotic resistance of enterococci, coagulase negative staphylococci and Staphylococcus aureus isolated from chicken meat." Czech Journal of Food Sciences 31, No. 1 (2013): 14–19. http://dx.doi.org/10.17221/58/2012-cjfs.

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We determined the antibiotic resistance of enterococci, coagulase negative staphylococci, and Staphylococcus aureus isolated from chicken meat samples. The antibiotic resistance of the isolated strains was estimated by the Kirby-Bauer disk diffusion method (according to the NCCLS document M2-A9 suggestions). It was found that all strains of Enterococcus spp. were resistant to tetracycline, 75% of them were resistant to ciprofloxacin, and 50% of them were resistant to erythromycin, vancomycin, and chloramphenicol. Also all strains of S. aureus were resistant to tetracycline and 25% of S. aureus

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Shlezinger, Mor, Shunit Coppenhagen-Glazer, Daniel Gelman, Nurit Beyth, and Ronen Hazan. "Eradication of Vancomycin-Resistant Enterococci by Combining Phage and Vancomycin." Viruses 11, no. 10 (2019): 954. http://dx.doi.org/10.3390/v11100954.

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Currently, effective options are needed to fight vancomycin-resistant Enterococcus faecalis (VRE). The present study shows that combinations of phage and vancomycin are highly efficient against VRE, despite being resistant to the antibiotic. Vancomycin-phage EFLK1 (anti-E. faecalis phage) synergy was assessed against VRE planktonic and biofilm cultures. The effect of the combined treatment on VRE biofilms was determined by evaluating the viable counts and biomass and then visualized using scanning electron microscopy (SEM). The cell wall peptidoglycan was stained after phage treatment, visuali

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Suchi, Surovi Era, SM Shamsuzzaman, Bhuiyan Mohammad Mahtab Uddin, and Md Abdullah Yusuf. "Detection of Virulence Factors and Antimicrobial Resistance in Enterococci Isolated from Urinary Tract Infection." Bangladesh Journal of Infectious Diseases 4, no. 2 (2018): 30–34. http://dx.doi.org/10.3329/bjid.v4i2.37682.

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Background: The genus Enterococcus is of increasing significance as a cause of nosocomial infections and this trend is exacerbated by the development of antibiotic resistance.Objective: The aims of this study was to find out the susceptibility pattern and the prevalence of virulence genes in Enterococcus strains isolated from urinary tract infection in Bangladesh.Methodology: This cross-sectional study was conducted in the Department of Microbiology at Dhaka Medical College, Dhaka from January 2015 to December 2015 for a period of one (01) years. All the admitted patients in the Dhaka Medical

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Różańska, Hanna, Aleksandra Lewtak-Piłat, Maria Kubajka, and Marcin Weiner. "Occurrence of enterococci in mastitic cow’s milk and their antimicrobial resistance." Journal of Veterinary Research 63, no. 1 (2019): 93–97. http://dx.doi.org/10.2478/jvetres-2019-0014.

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Abstract Introduction: The aim of the study was to evaluate the occurrence of enterococci in inflammatory secretions from mastitic bovine udders and to assess their antimicrobial resistance. Material and Methods: A total of 2,000 mastitic milk samples from cows were tested in 2014–2017. The isolation of enterococci was performed by precultivation in buffered peptone water, selective multiplication in a broth with sodium azide and cristal violet, and cultivation on Slanetz and Bartley agar. The identification of enterococci was carried out using Api rapid ID 32 strep kits. The antimicrobial sus

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LU, J. J., C. L. PERNG, T. S. CHIUEH, et al. "Detection and typing of vancomycin-resistance genes of enterococci from clinical and nosocomial surveillance specimens by multiplex PCR." Epidemiology and Infection 126, no. 3 (2001): 357–63. http://dx.doi.org/10.1017/s0950268801005453.

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Ninety-three clinical isolates of vancomycin-resistant enterococci (VRE) collected from nine hospitals in Taiwan were examined for the presence of vanA, vanB, vanC1, or vanC2/vanC3 genes by a multiplex PCR. Forty-seven of these VRE isolates were vanA positive, 1 contained both vanC1 and vanA, 40 harboured vanB, 2 were vanC1, and 3 were identified to be vanC2/vanC3. Twenty-four vanA isolates were sensitive to teicoplanin and thus did not have a typical VanA phenotype. Five isolates with the VanC phenotype harboured vanB. None of the 40 clinically isolated vancomycin-susceptible E. faecium or E.

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Baptista, M., F. Depardieu, P. Courvalin, and M. Arthur. "Specificity of induction of glycopeptide resistance genes in Enterococcus faecalis." Antimicrobial Agents and Chemotherapy 40, no. 10 (1996): 2291–95. http://dx.doi.org/10.1128/aac.40.10.2291.

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Regulation of VanA- and VanB-type glycopeptide resistance in enterococci is mediated by related two-component regulatory systems (VanR-VanS and VanRB-VanSB). The transglycosylase inhibitors vancomycin, teicoplanin, and moenomycin induced synthesis of the VanX D,D-dipeptidase in a VanA-type Enterococcus faecalis harboring transposon Tn1546. Inhibitors of reactions immediately preceding (ramoplanin) or following (penicillin G and bacitracin) transglycosylation were not inducers. These results identify accumulation of membrane-bound lipid intermediate II as a potential signal for induction of Van

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Sweeney, Michael T., and Gary E. Zurenko. "In Vitro Activities of Linezolid Combined with Other Antimicrobial Agents against Staphylococci, Enterococci, Pneumococci, and Selected Gram-Negative Organisms." Antimicrobial Agents and Chemotherapy 47, no. 6 (2003): 1902–6. http://dx.doi.org/10.1128/aac.47.6.1902-1906.2003.

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ABSTRACT The activities of linezolid, an oxazolidinone antibacterial agent active against gram-positive organisms, alone and in combination with 35 antimicrobial agents were tested in vitro against methicillin-sensitive (n = 1 to 2 strains) and methicillin-resistant (n = 8 to 10) Staphylococcus aureus strains; vancomycin-sensitive (n = 6) and vancomycin-resistant (n = 6 to 8) Enterococcus faecalis strains; vancomycin-sensitive (n = 5) and vancomycin-resistant (n = 6) Enterococcus faecium strains; penicillin-sensitive (n = 2 to 5), penicillin-intermediate (n = 5 to 6), and penicillin-resistant

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Hill, Craig M., Kevin M. Krause, Stacey R. Lewis, et al. "Specificity of Induction of the vanA and vanB Operons in Vancomycin-Resistant Enterococci by Telavancin." Antimicrobial Agents and Chemotherapy 54, no. 7 (2010): 2814–18. http://dx.doi.org/10.1128/aac.01737-09.

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ABSTRACT Telavancin is a bactericidal, semisynthetic lipoglycopeptide indicated in the United States for the treatment of complicated skin and skin structure infections caused by susceptible Gram-positive bacteria and is under investigation as a once-daily treatment for nosocomial pneumonia. The related vanA and vanB gene clusters mediate acquired resistance to glycopeptides in enterococci by remodeling the dipeptide termini of peptidoglycan precursors from d-alanyl-d-alanine (d-Ala-d-Ala) to d-alanyl-d-lactate (d-Ala-d-Lac). In this study, we assessed the ability of telavancin to induce the e

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Wardoyo, Eustachius Hagni, Didit Yudhanto, I. Gede Yasa Asmara, Claresta Salsabila, and Ajeng Retno. "Analysis of the Restriction of Vancomycin Use in Hospitals Before, During and After the Implementation of the Antimicrobial Resistance Control Program (ARCP)." Indonesian Journal of Infectious Diseases 6, no. 2 (2020): 1. http://dx.doi.org/10.32667/ijid.v6i2.109.

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Background: A policy restricting the use of vancomycin was a pilot project for the implementation of the Antimicrobial Resistance Control Program (ARCP) in RSUD Nusa Tenggara Barat in 2018. There were three phases of the ARCP in hospitals, namely preparation, execution, and monitoring. This research aimed to evaluate the quality of vancomycin prescribing before, during, and after the implementation of ARCP in 2017–2019. Method: Descriptive analysis was conducted of all the vancomycin prescriptions before, during, and after the implementation of ARCP in 2018. All medical records of cases requir

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Bourouni, Ouissal Chahad, Monia El Bour, Pilar Calo-Mata, Radhia Mraouna, Boudabous Abedellatif, and Jorge Barros-Velàzquez. "Phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass Dicentrarchus labrax." Canadian Journal of Microbiology 58, no. 4 (2012): 463–74. http://dx.doi.org/10.1139/w2012-014.

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The use of lactic acid bacteria (LAB) in the prevention or reduction of fish diseases is receiving increasing attention. In the present study, 47 LAB strains were isolated from farmed seabass ( Dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16S rDNA and randomly amplified polymorphic DNA – polymerase chain reaction (RAPD–PCR). Their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. Most of the strains isolated were enterococci belonging to the following species: Enterococcus faecium (59%), Enterococcus faecalis

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Neely, Alice N., and Matthew P. Maley. "Survival of Enterococci and Staphylococci on Hospital Fabrics and Plastic." Journal of Clinical Microbiology 38, no. 2 (2000): 724–26. http://dx.doi.org/10.1128/jcm.38.2.724-726.2000.

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The transfer of gram-positive bacteria, particularly multiresistantStaphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), among patients is a growing concern. One critical aspect of bacterial transfer is the ability of the microorganism to survive on various common hospital surfaces. The purpose of this study was to determine the survival of 22 gram-positive bacteria (vancomycin-sensitive and -resistant enterococci and methicillin-sensitive and -resistant staphylococci) on five common hospital materials: smooth 100% cotton (clothing), 100% cotton terry (towels), 60% cotton–40

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Soni, Manasi Manishbhai, Jitendrakumar Bhogilal Nayak, Pranav Ashok Anjaria, Prakrutik Prafulchandra Bhavsar, Jeetendrakumar Harnathbhai Chaudhary, and Manoj Natvarlal Brahmbhatt. "Isolation and molecular detection of biofilm producing and multiple drug resistant Enterococcus faecalis from the buffalo meat." Buffalo Bulletin 41, no. 3 (2022): 537. http://dx.doi.org/10.56825/bufbu.2022.4134917.

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The present study was conducted to determine the drug resistance pattern and the occurrence of biofilm producing Enterococcus faecalis from buffalo meat samples collected from in and around the Anand city, Gujarat. A total of 100 samples (meat, hand’s swab, knife’s swab) were collected aseptically from the butcher shops. Out of 100 samples, 52 (52%) samples were found to be positive on selective media, which were subjected to polymerase chain reaction revealing that 40 isolates were of Enterococcus faecalis. Antibiotic sensitivity test showed that all the isolates were sensitive to Ampicillin

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Azizi, Mohsen, Hamid Motamedi, Hadi Hossainpour, et al. "Rapid Detection of vanA Resistance Gene from E. faecalis Clinical Isolates Using Duplex Loop-Mediated Isothermal Amplification and Triplex PCR Assay." BioMed Research International 2022 (September 20, 2022): 1–8. http://dx.doi.org/10.1155/2022/4384196.

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Today, the spread of vancomycin-resistant strains isolated from Enterococcus faecalis (E. faecalis) has become a major health concern worldwide. Therefore, it is essential to provide a rapid and sensitive assay for identifying vanA gene for timely and appropriate antimicrobial control of resistant enterococcal infections. For this purpose, a cross-sectional study was performed on different clinical specimens of enterococci from Imam Reza hospital, Kermanshah, Iran. The antimicrobial susceptibility testing was determined by disk diffusion and MIC methods. Triplex-PCR and duplex-LAMP assays were

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Różańska, Hanna, Aleksandra Lewtak-Piłat, and Jacek Osek. "Antimicrobial resistance of Enterococcus faecalis isolated from meat." Bulletin of the Veterinary Institute in Pulawy 59, no. 2 (2015): 229–33. http://dx.doi.org/10.1515/bvip-2015-0034.

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Abstract The aim of the study was the evaluation of the antimicrobial resistance of Enterococcus faecalis strains isolated from cattle, pig, and poultry meat. A test was performed on 111 strains using the minimum inhibitory concentration technique. The highest number of isolates (94 strains) were resistant to lincomycin, the second-highest resistance was to quinupristin/dalfopristin (88 strains), tetracycline followed (65 strains), and erythromycin resistance was also notable (40 strains). All isolates tested were sensitive to daptomycin, nitrofurantoine, and tigecycline, whereas only few stra

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Psomas, E., I. Sakaridis, E. Boukouvala, et al. "Studying enterococci isolated from artisanal feta produced with mountain and low land milk." Journal of the Hellenic Veterinary Medical Society 75, no. 3 (2024): 7967–76. http://dx.doi.org/10.12681/jhvms.36209.

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Enterococci are commonly part of natural milk cultures that are used to produce cheese and have a strong influence on ripening, and aroma development in feta cheese. Despite their unique benefits in cheese production, Enterococci have also been identified as opportunistic pathogens that can cause various human diseases and their use remains controversial due to the possibility of antimicrobial resistance transfer to human strains. The objective of the present study was to detect, identify, and analyze characteristics of Enterococcus spp. from different samples of the artisanal feta cheese prod

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Krahulcová, Monika, Kristína Lépesová, and Lucia Bírošová. "Occurence of antibiotic resistant bacteria in raw cow milk from vending machines." Acta Chimica Slovaca 11, no. 1 (2018): 55–59. http://dx.doi.org/10.2478/acs-2018-0009.

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Abstract The prevalence of antibiotic resistant coliform bacteria and enterococci in fresh raw cow milk obtained from four vending machines in Slovakia was assessed. Also, differences in the number of antibiotic-resistant bacteria in milk according to season (winter and summer) were compared. Number of total coliform bacteria ranged from 2.45 to 4.18 log CFU/mL. Majority of them were resistant to ampicillin. This was expected due to their intrinsic resistance apart from Escherichia coli which is sensitive to ampicillin. In addition, we observed also tetracycline and gentamicin resistance. Each

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Bhatta, Sabita, Babli Basu, Chandrasekhar Narharrao Chaudhary, and Ashok Kumar Praharaj. "In Vitro Antimicrobial Activity of Tigecycline." Medical Journal of Shree Birendra Hospital 13, no. 2 (2015): 35–38. http://dx.doi.org/10.3126/mjsbh.v13i2.13114.

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Introduction: Tigecycline is a novel glycylcycline derivative of the tetracycline with activity against a wide range of organisms including Methicillin resistant Staphylococcus aureus, Vancomycin resistant Enterococcus , Extended spectrum beta lactamase producing (Escherichia coli , Klebsiella pneumonia) and Acinetobacter species. The aim of the study was to assess effectiveness of the drug against methicillin resistant Staphylococcus aureus (MRSA), vancomycin resistant enterococci (VRE), ESBL producers and carbapenem resistant Acinetobacter baumannii and to compare the efficacy of different m

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Franyó, Dorottya, Balázs Kocsi, Evelin Erzsébet Bukta, Judit Szabó, and Zsuzsanna Dombrádi. "Assessing the intestinal carriage rates of vancomycin-resistant enterococci (VRE) at a tertiary care hospital in Hungary." Folia Microbiologica 65, no. 3 (2019): 483–90. http://dx.doi.org/10.1007/s12223-019-00751-x.

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Abstract Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. Stool samples sent for routine culturing were screened with vancomycin containing solid or broth enrichment media. VRE isolates were identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and antibiotic susceptibilities were tested by

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Soni, Manasi M., Pranav A. Anjaria, Prakrutik P. Bhavsar, Jitendrakumar B. Nayak, Jeetendrakumar H. Chaudhary, and Manoj N. Brahmbhatt. "Isolation and Molecular Detection of Enterococcus faecalis from Cow Milk of Anand City with Special Reference to." Indian Journal of Veterinary Sciences & Biotechnology 18, no. 4 (2022): 6–9. http://dx.doi.org/10.48165/ijvsbt.18.4.02.

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The present study was conducted to determine the drug resistance pattern and the occurrence of biofilm-producing Enterococcus faecalis from cow milk samples collected from in and around Anand city, Gujarat. A total of 100 samples were collected aseptically from the doorsteps of livestock farmers. Out of 100 samples, 73 (73%) samples were found to be positive on selective media, which was confirmed by polymerase chain reaction, which revealed that all the 73 isolates were of Enterococcus faecalis. The antibiotic sensitivity test showed that all the isolates were sensitive to ampicillin (100%) o

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Braak, Nicole van den, Alewijn Ott, Alex van Belkum, et al. "Prevalence and Determinants of Fecal Colonization with Vancomycin-ResistantEnterococcusin Hospitalized Patients in The Netherlands." Infection Control & Hospital Epidemiology 21, no. 8 (2000): 520–24. http://dx.doi.org/10.1086/501797.

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Objective:To determine the prevalence and determinants of fecal carriage of vancomycin-resistant enterococci (VRE) in intensive care unit (ICU), hematology-oncology, and hemodialysis patients in The Netherlands.Design:Descriptive, multicenter study, with yearly 1-week point-prevalence assessments between 1995 and 1998.Population:All patients hospitalized on the testing days in ICUs and hematology-oncology wards in nine hospitals in The Netherlands were included.Methods:Rectal swabs obtained from 1,112 patients were screened for enterococci in a selective broth and subcultured on selective medi

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Gedik, Habip, Taner Yıldırmak, Funda Şimşek, et al. "Vancomycin-resistant enterococci colonization and bacteremia in patients with hematological malignancies." Journal of Infection in Developing Countries 8, no. 09 (2014): 1113–18. http://dx.doi.org/10.3855/jidc.4451.

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Introduction: We retrospectively evaluated the rates of vancomycin-resistant enterococci (VRE) colonization and VRE-related infections in patients with hematological malignancies. Methodology: All patients in the hematology department of the Ministry of Health Okmeydanı Training and Research Hospital, an 800-bed tertiary hospital in İstanbul, Turkey, older than 14 years of age and who developed febrile neutropenia during chemotherapy for hematological cancers between November 2010 and November 2012 were evaluated in this retrospective observational study. Results: A total of 282 neutropenic ep

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Trivedi, K., R. Sedmíková, and R. Karpíšková. "Bacteriocin activity of enterococci and presence of genes related to pathogenesis." Czech Journal of Food Sciences 30, No. 4 (2012): 330–35. http://dx.doi.org/10.17221/181/2011-cjfs.

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In total 228 enterococci strains isolated from food were studied. Enterococcus faecalis, E. faecium, and E. casseliflavus were found to be the dominant strains while E. durans and E. mundtii were present in a smaller extent. Antimicrobial activity determined by double layer technique revealed that 150 (65.7%) strains showed antimicrobial activity against the individual tested pathogenic strains of Listeria monocytogenes, Staphylococcus aureus, and methicilin resistant S.&nbsp;aureus (MRSA). Cell-free neutralised supernatants (CFNS) were prepared from 150 potential bacteriocin producers. Of

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Alder, Jeff, Tongchaun Li, Donghui Yu, et al. "Analysis of Daptomycin Efficacy and Breakpoint Standards in a Murine Model of Enterococcus faecalis and Enterococcus faecium Renal Infection." Antimicrobial Agents and Chemotherapy 47, no. 11 (2003): 3561–66. http://dx.doi.org/10.1128/aac.47.11.3561-3566.2003.

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ABSTRACT Daptomycin efficacy against clinical isolates of Enterococcus faecalis, Enterococcus faecium, and a lab-derived daptomycin-resistant isolate of E. faecalis was investigated in a mouse model of renal infection. The daptomycin MICs against these enterococci ranged from 0.5 to 50 μg/ml. The objective of this study was to determine the relationship between the MICs of drugs against E. faecalis and E. faecium and the level of daptomycin exposure needed to evaluate the drug's efficacy. Correlating the required therapeutic exposures of mice with the exposures achieved clinically allowed us t

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Wada, Yusuf, Azian Harun, Chan Yean Yean, and Abdul Rahman Zaidah. "A Nanoplex PCR Assay for the Simultaneous Detection of Vancomycin- and Linezolid-Resistant Genes in Enterococcus." Diagnostics 13, no. 4 (2023): 722. http://dx.doi.org/10.3390/diagnostics13040722.

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Background: Enterococci are Gram-positive cocci found in the guts of humans and animals. The goal of this research is to develop a multiplex PCR assay that can detect the Enterococcus genus, four VRE genes, and three LZRE genes simultaneously. Methods: Primers used in this study were specifically designed for the detection of 16S rRNA of Enterococcus genus, vanA—vanB—vanC—vanD for vancomycin, cfr methyltransferase, and optrA, and poxtA, as well as an adenosine triphosphate-binding cassette (ABC) transporter for linezolid. A Vibrio cholerae ctxA (internal amplification control) was included. Op

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Mamtora, Dhruv, Sanjith Saseedharan, Pallavi Bhalekar, and Surekha Katakdhond. "Microbiological profile and antibiotic susceptibility pattern of Gram-positive isolates at a tertiary care hospital." Journal of Laboratory Physicians 11, no. 02 (2019): 144–48. http://dx.doi.org/10.4103/jlp.jlp_173_18.

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Abstract OBJECTIVES: Gram-positive infections such as those by Staphylococcus aureus have contributed to the disease burden by increasing the morbidity and mortality rates in India. This study aims to determine the prevalence and the antibiotic susceptibility pattern of Gram-positive pathogens at a tertiary care hospital, Mumbai, Maharashtra, India. MATERIALS AND METHODS: This retrospective cross-sectional study was carried out from January, 2015 to December, 2017, at a tertiary care hospital in Mumbai, India. The clinical isolates were cultured, and identification was done using Vitek 2 cultu

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