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1

Stella, Eguono Oritsegbubemi Osaide, Ukolobi Odiri, and Pela-Borke Oghenechovwen. "Nutritional Value of Bitter Leaf (Vernonia Amygdalina) and Scent Leaf (Ocimum Gratissimum)." GVU Journal of Research and Development 1, no. 2 (2024): 54–63. https://doi.org/10.5281/zenodo.14031838.

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Most vegetables, including Vernonia amygdalina (bitter leaf) and Ocimum gratissimum (scent leaf), play a vital role in supporting the healthy development of human organs. This study compared the nutritional values of these two vegetables through mineral and proximate analyses. Fresh samples of bitter leaf (Vernonia amygdalina) and scent leaf (Ocimum gratissimum) were obtained from a personal farm in Edjeba, located in the Warri South Local Government Area of Delta State. The vegetables were air-dried at room temperature, ground, and sieved using a 2mm sieve, then stored in sterile Ziploc bags
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2

Putri, Adira Aulia, Irdat Mukaromah, Novi Dwi Pratiwi, and Galih Noor Alivian. "UTILIZATION OF AFRICAN LEAVES (VERNONIA AMYGDALINA) TO LOWER BLOOD PRESSURE IN PATIENTS WITH HYPERTENSION: A SYSTEMATIC REVIEW." International Journal of Biomedical Nursing Review 1, no. 1 (2022): 52. https://doi.org/10.20884/1.ijbnr.2022.1.1.6534.

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Introduction: Hypertension is a non-communicable disease caused by genetics, stress, and lack of physical activity. So far, no effective drugs have been found to cure high blood pressure. Vernonia amygdalina contains substances that can lower blood pressure, namely flavonoids and potassium. Purpose: The systematic review aims to assess the potential of vernonia amygdalina in lowering blood pressure in hypertensive people. Methods: Articles are obtained from electronic databases such as PubMed, ScienceDirect, and Google Scholar published from 2011 to 2021. Keywords used: ("African leaf" OR "bit
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3

Abdulrahman, A., M. G. Garba, S. Gaddafi, and A. Yusuf. "RESPONSE OF YANKASA RAMS TO VARIOUS INCLUSION LEVELS OF PROPAGATED BITTER LEAF (Vernonia amygdalina) ON PERFORMANCE AND RUMEN ECOLOGY." FUDMA Journal of Animal Production and Environmental Science 1, no. 1 (2025): 46–51. https://doi.org/10.33003/japes.2025.v1i1.46-51.

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The experiment evaluated the response of Yankasa rams fed graded levels of propagated Bitter Leaf (Vernonia amygdalina) on growth performance, nutrient digestibility, rumen ecology, and blood profile. Twenty (20) rams were randomly allocated into four dietary treatments consisting of 0%, 5%, 10%, and 15% inclusion levels of dry bitter leaf. Growth performance, nutrient digestibility trail and rumen fluid were collected and analyzed accordingly. Data obtained were analyzed using statistical analysis system (SAS,2002). Growth performance results showed that T3(15%) had an outstanding (P<0.05)
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4

Oseghale, Ikalo David, Olabisi Promise Lawal, Daniel Osezuwa Ubebe, et al. "Ethnomedicinal and Phytopharmacological Aspects of Vernonia amygdalina (Bitter Leaf) Utilized as a Traditional Medicinal Herb." Asian Journal of Research in Biochemistry 14, no. 6 (2024): 41–57. http://dx.doi.org/10.9734/ajrb/2024/v14i6326.

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Vernonia amygdalina Delile, commonly known as “bitter leaf,” is a shrub in the Asteraceae family, widely seen in tropical regions of Africa. Vernonia amygdalina is found to be traditionally used in African medicine. Various parts of this plant, especially the leaves, are utilized to treat ailments such as fever, diabetes, and gastrointestinal issues. Research from multiple researchers has shown a wide range of phytochemical and bioactive compounds present in Vernonia amygdalina; examples are terpenoids, tannins, alkaloids, saponins, flavonoids, steroids, glycosides, etc., which have contribute
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5

Obi, P. U., Mohammed Babagana, I. Idris, M. Hadiza, E. M. Nma, and A. Nadhiekhan. "Analysis of Proximate, Mineral and Phytochemical Composition of fresh and dry Vernonia amygdalina (Bitter Leaf) in Bida Metropolis, Niger State." UMYU Scientifica 3, no. 1 (2024): 88–94. http://dx.doi.org/10.56919/usci.2431.010.

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Vernonia amygdalina is tropical shrub with high biomass yield which is used to prepare several dishes, it is also utilized as herb for health improvement. This study was carried out to determine, the proximate, mineral and phytochemicals composition of fresh and dry bitter leaf (Vernonia amygdalina) leaves in Bida metropolis. The Vernonia amygdalina leaf samples were evaluated using standard procedures. The result of the proximate analysis show that percentage dry matter and moisture content shows significant (p<0.05) difference between the samples. The percentage ash content and the crude
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6

Okanume, Ogochukwu Esther, Solomon Gabriel Abok, and Oluwatobi Adekunle Oso. "Leaf epidermal and petiole anatomical features as taxonomic characters in some Vernonia species in Nigeria." Bio-Research 20, no. 1 (2022): 1388–97. http://dx.doi.org/10.4314/br.v20i1.1.

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Foliar and petiole micro-morphological characteristics of some members of the genus Vernonia namely; Vernonia amygdalina Del. (bitter variety), Vernonia amygdalina Del. (non-bitter variety), Vernonia cinerea (L) Less., Vernonia galamensis (Cass.) Less., and Vernonia adoensis Sch. Bip. in Jos, Nigeria was investigated to provide additional micro-morphological characters to support existing taxonomic information regarding the species. Epidermal peels of fresh leaves were made and transverse sections of petioles were cut using a rotary microtome. Staining was done using Safranin and slides observ
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7

Iyama, William, Kingsley Okpara, and Kuaanan Techato. "Assessment of Heavy Metals in Agricultural Soils and Plant (Vernonia amygdalina Delile) in Port Harcourt Metropolis, Nigeria." Agriculture 12, no. 1 (2021): 27. http://dx.doi.org/10.3390/agriculture12010027.

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This study assessed the concentration of heavy metal, such as lead (Pb), cadmium (Cd), Chromium (Cr), iron (Fe), Nickel (Ni), and Silver (Ag), in Vernonia amygdalina Delile and agricultural soils of three university farms located in Port Harcourt, Nigeria. The soils and plants were taken randomly to form composite samples and analyzed for heavy metals by the use of atomic absorption spectroscopy (AAS) and X-ray fluorescence (XRF). The study stations were agricultural soils and Vernonia amygdalina Delile from the Ignatius Ajuru University of Education (I), River State University (R) and Univers
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8

Alege, Gbenga Olorunshola, Musa Ozigi Yakubu, Deborah Tini Egilla, David Franklin Igata, Usman Ibrahim Aliyu, and Danlami Dauda. "GROWTH AND YIELD RESPONSE OF SESAME (SESAMUM INDICUM L.) TO BITTER LEAF (VERNONIA AMYGDALINA DEL.) LEAF, STEM AND ROOT AQUEOUS EXTRACTS." FUDMA JOURNAL OF SCIENCES 5, no. 4 (2022): 150–56. http://dx.doi.org/10.33003/fjs-2021-0504-798.

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This study was carried out to assess the effect of leaf, stem and root aqueous extracts of Vernonia amygdalina on seed germination, growth and yield performance of Sesamum indicum. The aqueous extracts of the three plant parts were applied at concentrations of 25%, 50%, 75%, and 100% to sesame seeds in petri dish and compared with seeds treated with distilled water (control). The percentage of germinated seeds and lengths of radicle and plumule were measure within 10 days of sowing. The different concentrations of leaf, stem and root extracts of Vernonia amygdalina were applied to the seedling
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9

Peter Achunike Akah. "Vernonia amygdalina Del (Bitter leaf) a traditional anti-diabetic gold mine - Mini Review." Journal of Ayurveda and Integrated Medical Sciences 9, no. 1 (2024): 170–75. http://dx.doi.org/10.21760/jaims.9.1.24.

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Diabetes mellitus is a worldwide epidemic that has serious consequences for public health. This in-depth analysis of Vernonia amygdalina, or Bitter Leaf, aims to shed light on the plant's potential anti-diabetic properties by discussing its botanical description, chemical composition, traditional uses, and mechanisms of action, scientific findings, safety concerns, and implications for future study. Vernonia amygdalina, a plant native to various nations in Africa, has gained popularity as a possible treatment for diabetes. Bitter leaf's traditional function in reducing diabetes-related complic
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10

Uthman H. "Kinetic Studies on the Inhibition Potential of Bitter Leaf (Vernonia Amygdalin) in the Management of Diabetes." GSC Advanced Research and Reviews 21, no. 3 (2024): 184–97. https://doi.org/10.30574/gscarr.2024.21.3.0483.

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The aim of this study was to investigate the inhibition potential of extracts from Vernonia amygdalina on α-amylase and α-glucosidase activity, and its useful mechanisms in the treatment of diabetes specifically Type 2. The searchlight is beamed on the potential of the plant extract to inhibit the activity of α-amylase and thereby determining its mechanism or mode on inhibition as well as its IC50 value. The inhibition of alpha-amylase and alpha-glucosidase enzymes is a key strategy in managing diabetes. Vernonia amygdalina is a natural plant extract that has been used traditionally to manage
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11

Raimi, C. O., W. A. Oyelade, and A. O. Iyanda. "Histopathological effect of aqueous bitter leaf extract (Vernonia amygdalina) on acetaminophen-induced liver damage in albino rat (Rattus norvegicus)." Nigerian Journal of Animal Production 47, no. 4 (2020): 24–31. http://dx.doi.org/10.51791/njap.v47i4.95.

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Liver diseases are a worldwide problem. Medicinal plants are being increasingly utilized to treat a wide variety of diseases, liver disorder inclusive. The effect of aqueous leaf extracts of Vernonia amygdalina on acetaminophen-induced liver damage via the assessment of the histopathological examination was examined. Five groups of albino rats were used (n = 5). Animals in group 1 were fed normal laboratory pellet and water ad libitum (Control); those in group 2 received 3000mg/kg of acetaminophen alone. Groups 3, 4 and 5 received 3000mg/kg of acetaminophen and in addition 50mg/kg Vernonia amy
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12

Cornelia, Melanie, Irene Agustini, and Marcelia Sugata. "Characterization of bitter leaf (Vernonia amygdalina Delile) extract, lemon (Citrus limon Osbeck), and honey in functional beverages making." BIO Web of Conferences 169 (2025): 04005. https://doi.org/10.1051/bioconf/202516904005.

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Bitter leaves (Vernonia amygdalina) are renowned for their health benefits, largely attributed to their polyphenol and flavonoid content, which contribute to their antioxidant properties. Traditionally, these leaves are consumed as a decoction to treat fever. In this study, the antioxidant compounds of bitter leaves were extracted using three different solvents—ethanol, ethyl acetate, and hexane—through maceration for 1, 2, and 3 days. The ethanol extract obtained after 2 days of maceration was selected for further analysis. To mitigate the inherent bitterness of bitter leaves (Vernonia amygda
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13

Zubairu, Aminu Yakubu, Muhammad Mukhtar, Isah Saidu, et al. "Antibacterial activity of methanolic extract of bitter leaf (Vernonia amygdalina) from various component fractions using column chromatography." GSC Biological and Pharmaceutical Sciences 7, no. 2 (2019): 016–21. https://doi.org/10.5281/zenodo.4286143.

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This research work determined the antibacterial activities of various components at different ratios from column fractions of methanolic extract of bitter leaf (<em>Vernonia amygdalina</em>), meanwhile column chromatography been the best method of separating component nowadays. The column chromatography was perform using several type of solvent such as non-polar, semi-polar, polar solvent as well as their mixture in deference ratio.&nbsp; The phytochemical screening was carried out on the leaves extract of (<em>Vernonia amygdalina</em>) which revealed the presence of some active ingredients su
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14

Ajayi, Abayomi, Titilayo Ige, Nich Arawa Ude, et al. "Aqueous extract of bitter leaf Vernonia amygdalina Delile (Asterales: Asteraceae) ameliorates testosterone-induced benign prostatic hyperplasia (BPH) in Wistar rats." Brazilian Journal of Biological Sciences 4, no. 7 (2017): 45–52. http://dx.doi.org/10.21472/bjbs.040706.

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Benign prostatic hyperplasia (BPH) is an age-dependent condition involving prostate enlargement which may lead to lower urinary tract symptoms (LUTS). This study was designed to study the ameliorative effect of the aqueous extract of bitter leaf Vernonia amygdalina Delile (Asterales: Asteraceae) on testosterone-induced BPH in a rat model. Thirty adult male Wister rats were randomly divided into six groups (A to F), with BPH induced through a single subcutaneous injection of 5 mg/kg of testosterone propionate (TP) and Vernonia amygdalina administered in various doses through oral gavage for 14
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15

ILESANMI Tolulope Margaret and FAJILADE Olawande Temilade. "Microbial assessment and lipid profile of broiler chicken treated with crude extract of Vernonia Amygdalina (Bitter Leaf)." Magna Scientia Advanced Biology and Pharmacy 14, no. 2 (2025): 115–25. https://doi.org/10.30574/msabp.2025.14.2.0030.

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Globally, broiler chickens are an essential source of protein, and their productivity and well-being have substantial economic implications. Bitter leaf (Vernonia amygdalina) has been used medicinally for a variety of reasons. The effects of bitter leaf extract, Vernonia amygdalina, on the lipid profile and microbial load of broiler chickens were examined in this study. Significant amounts of bioactive components, such as phenols (152.70 mg/100g), alkaloids (98.73 mg/100g), flavonoids (73.93 mg/100g), tannins (51.93 mg/100g), and saponins (60.26 mg/100g), were found in the crude extract upon p
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16

Magaji, Auwal, Zinat Mahmud, and Aminu Mustafa. "Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates." UMYU Journal of Microbiology Research (UJMR) 8, no. 2 (2023): 174–80. http://dx.doi.org/10.47430/ujmr.2382.020.

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Vernonia amygdalina is a plant known to contain substances with reported antimicrobial properties against various microorganisms. This study therefore, aimed at assessing the antibacterial properties of Vernonia amygdalina extract against some clinical bacterial isolates. The bacterial isolates were obtained from General hospital Azare, and the Vernonia amygdalina leaves were purchased at Azare central market. Various compounds, including saponins, glycosides, tannins, flavonoids, steroids, phenolic compounds, and alkaloids, were identified in the plant extract via phytochemical screening. Dif
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17

Dewi, Irene, Dachriyanus, Yufri Aldi, Nor Ismail, and Fatma Wahyuni. "Anti-inflammatory Potential of Vernonia amygdalina Delile Extract: In Vitro Studies." Tropical Journal of Natural Product Research 9, no. 4 (2025): 1381. https://doi.org/10.26538/tjnpr/v9i4.3.

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Vernonia amygdalina, commonly known as bitter leaf, widely used in traditional medicine across Africa and Asia, and is characterized by its bitter taste due to the presence of bioactive compounds such as flavonoids, alkaloids, saponins, and terpenoids. The study aimed to evaluate the effectiveness of Bitter Leaf (Vernonia amygdalina Delile) extract in suppressing pro-inflammatory cytokines and reducing phagocytic activity in cell-based assays. The Bitter leaf extract was prepared through a maceration process with ethanol solvent. To assess the anti-inflammatory potential of the extract, RAW 26
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18

Udeh, Winifred C., and Onyebuchi Obia. "Effect of Aqueous Extract of Vernonia amygdalina (Bitter Leaf) on GASTRIC PH and Serum Bicarbonate of Wistar Rats." INTERNATIONAL JOURNAL OF MEDICAL EVALUATION AND PHYSICAL REPORT 7, no. 2 (2023): 1–12. http://dx.doi.org/10.56201/ijmepr.v7.no2.2023.pg1.12.

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Vernonia amygdalina is a shrub commonly consumed in Nigeria as a vegetable in soup. The aim of the present study was to determine the effect of aqueous extract of Vernonia amygdalina on gastric pH and post-prandial serum bicarbonate in wistar rats. A total of 20 male wistar rats weighing 180-250g were divided into four groups of 5 rats each; Group I served as control, Group II received 400mg/kg Aqueous bitter leaf extract, Group III received Omeprazole (20mg/kg) and Group IV received a combination of Omeprazole and Aqueous bitter leaf extract. 45 minutes after administration of the extract, th
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19

Pol, Sagar Laxman, Pratima Singh, Mihir Otia, Sunil Kothari, Shubham K. Mohata, and Jay Prakash. "Phytochemistry and Pharmacological Activities of Vernonia amygdalina." Journal for Research in Applied Sciences and Biotechnology 3, no. 3 (2024): 135–41. http://dx.doi.org/10.55544/jrasb.3.3.22.

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Vernonia amygdalina is a small tree that can grow to a height of ten metres. It is sometimes referred to as bitter leaf due to the fact that its leaves have a bitter bitter flavour and its branches are frail. Although its natural habitat is in tropical Africa, it may be found all over the world in regions that receive an average rainfall of 750–2000 millimetres. These regions include areas that are adjacent to rivers and lakes, as well as forests and grasslands that are located up to an elevation of 2800 metres. The plant is mostly employed as a medicinal herb in the context of traditional med
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Okwara, V. C., H. A. Njoku, G.A Iroatumere, K. R. Oleru, and E. O. Obeten. "The Impact of Incorporating Bitter Leaf (Vernonia Amygdalina) as an Additive in Poultry Feed." International Journal of Research 12, no. 2 (2025): 404–12. https://doi.org/10.5281/zenodo.14875430.

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This study investigates the potential benefits of incorporating bitter leaf (Vernonia amygdalina) as a natural feed additive in poultry nutrition. A total of 240 birds were randomly assigned to 4 dietary treatments, including a control group and groups supplemented with varying levels of bitter leaf (0.5%, 1%, and 1.5%). Results showed significant improvements in growth rate (+12%), feed efficiency (+9%), and egg production (+15%) in birds fed bitter leaf-supplemented diets. Bitter leaf addition also enhanced gut health, indicated by increased beneficial gut microbiota and reduced pathogenic b
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Agbidye, Isaac Gbaa, Timothy Tertsea Awuhe, and Susan Doofan Iortyom. "Comparative Proximate and Phytochemical Analyses of the Leaf Extracts of two Species of Vernonia." Chemistry Research Journal 5, no. 2 (2020): 185–94. https://doi.org/10.5281/zenodo.12493111.

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<strong>Abstract </strong>Comparative proximate and phytochemical analyses of two variants of bitter leaf; <em>Vernonia </em><em>amygdalina</em> and <em>Vernonia colorata</em><em> </em>were studied using AAS, UV, IR and TLC. Extraction was done with ethanol using the powdered leaves of the two variants, distilled off, and labelled as: ethanol extract of <em>Vernonia amygdalina </em>(EEVA) and ethanol extracts of <em>Vernonia colorata </em>(EEVC). The same procedure was repeated with acetone and water to obtain: acetone extracts of <em>Vernonia amygadalina</em><em> </em>(AEVA); acetone extracts
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Adoyi, Michael Paul, Ubelejit Alfred John, Ladan Muhammad Suwaiba, Samuel Terhide Tyohemba, Vawanje Bitrus Kwaya, and Asugu Mary Mbahi. "COMPARATIVE EVALUATION OF THE ANTIBACTERIAL EFFICACY AND PHYTOCHEMICAL PROFILE OF METHANOL AND AQUEOUS EXTRACTS OF Vernonia amygdalina AGAINST GASTROENTERITIS CAUSING BACTERIA." FUDMA JOURNAL OF SCIENCES 9, no. 4 (2025): 161–66. https://doi.org/10.33003/fjs-2025-0904-3564.

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Vernonia amygdalina, commonly known as bitter leaf, is a shrub recognized for its bioactive compounds with documented antibacterial properties against gastroenteritis. However, the efficacy of methanolic and aqueous extracts of Vernonia amygdalina leaves has not been extensively evaluated. This study aimed to assess the efficacy of these extracts and determine their chemical composition, as well as their potential to inhibit the activity of selected bacteria associated with gastroenteritis. Vernonia amygdalina leaves were procured from Gombe central market, and the extracts were subjected to p
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Ali, Muhammad, Sani U. Diso, Sumayya A. Waiya, and Muhammad S. Abdallah. "Phytochemical Screening and Antibacterial Activity of Bitter Leaf (Vernonia amygdalina)." Annals of Microbiology and Infectious Diseases 2, no. 4 (2019): 1–7. http://dx.doi.org/10.22259/2637-5346.0204001.

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Opara, A., R. Egbuobi, J. Ndudim, C. Onyewuchi, and J. Nnodim. "Antibacterial Activity of Ocimum gratissimum (Nchu-Anwu) and Vernonia amygdalina (Bitter-Leaf) Antibacterial Activity of Ocimum gratissimum (Nchu-Anwu) and Vernonia amygdalina (Bitter-Leaf)." British Biotechnology Journal 4, no. 10 (2014): 1115–22. http://dx.doi.org/10.9734/bbj/2014/11995.

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Safitri, Amelia Dwita, Iwan Sahrial Hamid, Poedji Hastutiek, Setiawan Koesdarto, Rahmi Sugihartuti, and Endang Suprihati. "The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro." Journal of Parasite Science 3, no. 1 (2019): 19. http://dx.doi.org/10.20473/jops.v3i1.16427.

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The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia ga
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EHIELU, R. O., H. I. AJAYI, J. I. IMOUOKHOME, and I. I. ILABOYA. "EFFECT OF Vernonia Amygdalina LEAF MEAL EXTRACT IN DRINKING WATER ON POST-WEANING DIARRHEA OCCURRENCE IN PIGLETS." FUDMA Journal of Agriculture and Agricultural Technology 9, no. 2 (2023): 29–39. http://dx.doi.org/10.33003/jaat.2023.0902.04.

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This study was aimed at determining the effect of Vernonia amygdalina leaf meal (VALM) extract in drinking water on the faecal microbial load on post-weaning diarrhea occurrence in piglets using different agar. Forty-five piglets of Large white X Duroc breed were given Vernonia amygdalina (VA) through water infusion. Three levels of 0.0g, 1.2g, and 2.4g of VA per 1000ml of clean drinking water designated as T1, T2, and T3 were used. Nine piglets weaned at different ages of 2nd, 3rd, 4th, 5th, and 6th week of age were randomly allotted to three treatments with each treatment replicated three ti
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Oshotse, R. B., and M. O. Ifeanacho. "Lipid Profile, Haematological Assay and Tissue Histology of Alloxan Induced Diabetic Wistar Rats Administered Extracts of Vernomia amygdalina (Bitter leaf) and Gnetum africanum (okazi leaf)." Journal of Applied Sciences and Environmental Management 25, no. 7 (2021): 1099–105. http://dx.doi.org/10.4314/jasem.v25i7.1.

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&#x0D; &#x0D; &#x0D; Diabetes mellitus is predominant in numerous nations of the world with millions of deaths directly linked to it. The utilization of plants in providing answers to this pandemic has expanded over the years. This study investigated changes in some haematological parameters and tissue histology of alloxan induced diabetic Wistar rats administered combined leaf extracts (CLE) of vernonia amygdalina (bitter leaf (BI)) and gnetum africanum (okazi leaf (OK)). Aqueous extracts of bitter leaf and okazi leaf were prepared using the conventional method. Forty Wistar rats were grouped
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Eraga, LI, E. Aganbi, AA Anigboro, SO Asagba, and NJ Tonukari. "Antigenotoxicity and antioxidant activities of bitter leaf (Vernonia amygdalina del.) accessions from different parts of Nigeria." African Journal of Food, Agriculture, Nutrition and Development 22, no. 4 (2022): 20161–80. http://dx.doi.org/10.18697/ajfand.109.20355.

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Bitter leaf (Vernonia amygdalina Del.) plant is a tree species that is highly cultivated in Nigeria for its nutritive and therapeutic values. This study aimed to determine the antioxidant and antigenotoxicity effects (in vitro) of 52 accessions of V. amygdalina collected from six geopolitical zones of Nigeria (North East, North West, North Central, South South, East and West) by evaluating the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO-) scavenging antioxidant activities, flavonoid and phenolic contents as well as ethidium bromide-induced DNA (deoxyribonucleic acid) damage of bi
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Erhabor, Aisosa, and Osayawe Erhabor. "Comparative Analysis of Phytochemical Composition of Aqueous Extracts from Azadirachta indica and Vernonia amygdalina." Sciences of Phytochemistry 3, no. 2 (2024): 91–97. http://dx.doi.org/10.58920/sciphy0302270.

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Azadirachta indica (neem) and Vernonia amygdalina (bitter leaf) are among the most promising medicinal plants, valued for their broad spectrum of biological activities, including antibacterial, antifungal, anti-inflammatory, antiviral, antidiabetic, and antitumor properties. These plants play a central role in traditional Nigerian medicine for treating tropical diseases such as malaria, typhoid fever, yellow fever, and scabies. This study employed gravimetric analysis to identify and quantify the phytochemical compounds in both plants. Fresh leaves were collected, cleaned, air-dried, and their
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Olosunde, A. O., and S. M. Odeyinka. "West African dwarf goats utilized bitter leaf (Vernonia amygdalina) meal as feed supplement." Nigerian Journal of Animal Production 44, no. 5 (2020): 250–56. http://dx.doi.org/10.51791/njap.v44i5.1483.

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This studywas carried out to determine the utilization and the optimumlevel of offer of bitter leaf (Vernonia amygdalina) meal (BLM) by West African Dwarf (WAD) goats. In a 20-week trial, twenty four WAD goats of both sexes, 5-7 months old, were randomly allotted to four treatments of graded levels of BLM(0% BLM(Control diet), 15% BLM, 30% BLMand 45% BLMdiets) in a completely randomized design to determine the utilization of bitter leafmeal as feed for goats. The dietswere used as supplements to a basal ration of Panicummaximum. Two digestibility trials were carried out. The digestible ether e
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Afuye, O. O., and S. O. Omotayo. "Evaluation of In vitro Anti-Diabetic Properties of Biosynthesized Magnesium Oxide Nanoparticles from Vernonia amygdalina (Bitter Leaf) Aqueous Leaf Extract." European Journal of Medicinal Plants 35, no. 1 (2024): 45–55. http://dx.doi.org/10.9734/ejmp/2024/v35i11178.

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Introduction: Vernonia amygdalina is a common shrub that is widely used and extracts from them have been traditionally used as remedies for treating diabetes mellitus in various parts of the world. The use of V. amygdalina to synthesize MgO nanoparticles has been used for various biomedical applications and it is compatible with anti-diabetic studies. This research investigates the in vitro anti-diabetic potentials of biosynthesized from aqueous V. amygdalina leaf extract. Methodology: Aqueous extract of V. amygdalina-MgO nanoparticles were characterized using Fourier Transform Infrared, X-ray
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Gospel, Ajuru, Onwuli Donatus, and Ajuru Mercy. "THE EFFECT OF Vernonia amygdalina Del. (BITTER LEAF) LEAF EXTRACT ON THE LIPID PROFILE OF WISTAR ALBINO RATS." Continental J. Biomedical Sciences 7, no. 1 (2013): 23–30. https://doi.org/10.5281/zenodo.6380020.

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This study investigates the effect of aqueous leaf extract of <em>Vernonia amygdalina </em>Del. (bitter leaf) on the lipid profile of Wistar rats. The rats were divided into 3 groups. Control group were given no leaf extract, low dose group were given 0.5ml of leaf extract and high dose group given 1ml of the extract. Animals given 1ml of extract had a highly significant decrease of Total-Chol, LDL-Chol and Triglycerides concentrations with mean and standard deviation of 112.89 &plusmn; 1.08, 69.71 &plusmn; 2.32 and 96.21 &plusmn; 0.71 (mg/dl) respectively compared with control group with mean
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Dienye, H. E. "Vernonia amygdalina (BITTER LEAF) EXTRACTS AS PRESERVATIVE FOR CATFISH (Clarias gariepinus)." International Journal of Nutritional Science and Food Technology 3, no. 1 (2017): 102–8. http://dx.doi.org/10.25141/2471-7371-2017-1.0102.

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Mohammed, A. A., and A. S. Zakariya`u. "Bitter Leaf (Vernonia amygdalina) as a Feed Additive in Broiler Diets." Research Journal of Animal Sciences 6, no. 3 (2012): 38–41. http://dx.doi.org/10.3923/rjnasci.2012.38.41.

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Samuel Ilesanmi, Olutosin, Omowumi Funke Adedugbe, David Adeniran Oyegoke, Rachael Folake Adebayo, and Oluwaseun Emmanuel Agboola. "Biochemical properties of purified polyphenol oxidase from bitter leaf (Vernonia amygdalina)." Heliyon 9, no. 6 (2023): e17365. http://dx.doi.org/10.1016/j.heliyon.2023.e17365.

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Raji, Modinat, Florence Kemi Amoo, Kamoru Abdulazeez Adeniyi, et al. "ANTIBACTERIAL ACTIVITY OF BITTER LEAF (Vernonia amygdalina) EXTRACTS ON Escherichia coli AND Staphylococcus aureus." FUDMA JOURNAL OF SCIENCES 9, no. 4 (2025): 155–60. https://doi.org/10.33003/fjs-2025-0904-3344.

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In recent years, there has been a growing global health concern, necessitating the search for potent, resistance-free medicinal plants with antibacterial properties. This study evaluates the phytochemical and antibacterial properties of Vernonia amygdalina (bitter leaf) extracts as an alternative treatment for bacterial infections. Ethanolic and aqueous extracts of V. amygdalina leaves were prepared and subjected to phytochemical screening, revealing the presence of quinones, tannins, steroids, saponins, terpenoids, alkaloids, phenols, and flavonoids. The antibacterial activity was assessed ag
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Musyoki, David. "Exploring the Antibacterial Properties of Vernonia amygdalina (Bitter Leaf) Extract: A Potential Alternative to Conventional Antibiotics." NEWPORT INTERNATIONAL JOURNAL OF BIOLOGICAL AND APPLIED SCIENCES 5, no. 1 (2024): 60–67. http://dx.doi.org/10.59298/nijbas/2024/5.1.606711.

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As part of the ongoing search for potent and resistance-free antibacterial medicinal plants, this study aimed to evaluate the antibacterial properties of the plant extract of Vernonia amygdalina, commonly known as bitter leaf. Standard procedures were used to provide a potential cheap alternative to conventional medication for treating bacterial infections. The aqueous extract of V. amygdalina leaves was prepared and subjected to phytochemical screening, which revealed the presence of tannins, phlobatannins, saponins, terpenoids, cardiac glycosides and alkaloids. The antibacterial activity of
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Adeiza, S. Shuaibu, and B. Shuaibu Abdulmalik. "Assessment of the Phytochemical constituents and in-vitro antibacterial activity of Vernonia amygdalina extracts on someclinical isolates." Pharmaceutical and Chemical Journal 4, no. 4 (2017): 123–28. https://doi.org/10.5281/zenodo.13785393.

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Antimicrobial resistance is on the rise and efforts are consequently being strengthened towards the search and improvement of sturdier antimicrobial agents with expectant safety and efficacy. This study was conducted to determine the phytochemical analysis and antibacterial activity of <em>Vernonia amygdalina </em>(bitter leaf) extracts obtained from a market in Sokoto state Nigeria on some clinical isolates. The efficiency of cold water, hot water and ethanol extracts on <em>Salmonella </em>Sp and <em>Escherichia coli</em> were evaluated by disc diffusion technique. Ethanolic extracts of <em>
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Biru, Meron Asteraye, Yasin Ahmed Waday, and Lema Deme Shumi. "Optimization of Essential Oil Extraction from Bitter Leaf (Vernonia Amygdalina) by Using an Ultrasonic Method and Response Surface Methodology." International Journal of Chemical Engineering 2022 (August 16, 2022): 1–6. http://dx.doi.org/10.1155/2022/4673031.

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Bitter leaf (Vernonia amygdalina) is a common bush or small tree that grows in tropical Africa. In the Ethiopian highland, the bitter leaf has been classified by the farmer as a versatile tree with high biomass yield and easy propagation. It is also well known in traditional medicine and nutritional use. The objective of this study was extraction and optimization of essential oil (EO) from the bitter leaf by using the ultrasonic extraction method and response surface methodology. The experiment was designed by Box–Behnken Design (BBD) with three factors to investigate the effect of sonication
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Boadu, A., S. Singh, R. Karpoormath, and M. Nlooto. "REVIEW ON ETHNOMEDICINAL USES, PHYTOCHEMICAL CONSTITUENTS AND PHARMACOLOGICAL EVIDENCE ON LEAF EXTRACT OF PERSEA AMERICANA AND VERNONIA AMYGDALINA OF THE AFRICAN CONTINENT - A REVIEW." INDIAN DRUGS 56, no. 01 (2019): 7–24. http://dx.doi.org/10.53879/id.56.01.11623.

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People on the African continent frequently consume Persea americana and Vernonia amygdalina for food and medicinal purposes. The review is an effort to compile ethnomedicinal, phytochemical and pharmacological knowledge is the above two plants from the year 2000 till now. A search was carried out using a combination of several keywords : Persea americana, Vermonia amygdalina, traditional medicine, ethnomedicine, avocado, bitter leaf and integrated approaches. Published papers in English language were retrieved from the online bibliographical databases: Web of Science, PubMed, Google Scholar, S
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Ogwulumba, Solomon, Kevin Ugwuoke, and Rufus Ogbuji. "Reaction of Tomato CV. Roma VF (Solanum Lycopersicum) to Meloidogyne Javanica Treub Infestation in an Ultisol Treated with Aqueous Leaf Extracts of Bitter Leaf (Vernonia Amygdalina L.) and Mango (Mangifera Indica L.)." Journal of Plant Protection Research 51, no. 1 (2011): 14–17. http://dx.doi.org/10.2478/v10045-011-0003-2.

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Reaction of Tomato CV. Roma VF (Solanum Lycopersicum) to Meloidogyne Javanica Treub Infestation in an Ultisol Treated with Aqueous Leaf Extracts of Bitter Leaf (Vernonia Amygdalina L.) and Mango (Mangifera Indica L.) The reaction of tomato cv. Roma vf (Solanum lycopersicum) to Meloidogyne javanica Treub infestation in an ultisol treated with aqueous leaf extracts of bitter leaf (Vernonia amygdalina L.) and mango (Mangifera indica L.) was investigated in the Federal College of Agriculture Ishiagu, Ebonyi State, SE Nigeria. Two pot experiments were performed in 2008 and 2009. The various leaf ex
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Suresh, Sushanthi, Srisakthi Doraikannan, Meignana Indiran, and S. Rajeshkumar. "Antioxidant, Anti-Inflammatory and Cytotoxic Effects of Vernonia Amygdalina Mediated Copper Nanoparticles." Journal of Complementary Medicine Research 13, no. 5 (2023): 1. http://dx.doi.org/10.5455/jcmr.2023.14.02.01.

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Background: Vernonia amygdalina is generally known as sour leaf or bitter leaf which is one among the medically significant plants in African and Mediterranean regions. Hence, this study aids the biosynthesis of copper nanoparticles using V.amygdalina for the first time and further it aims to investigate the cytotoxicity, antioxidant and anti-inflammatory properties of the V.amygdalina mediated copper nanoparticles. Methods: Cytotoxic effect of Copper sulphate nanoparticles reinforced with Vernonia amygdalina extract were assessed using Brine Shrimp Assay at 5 µL, 10 µL, 20 µL, 40µL and 80µL,
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Ekpenyong, Nsikak Edet, Ubong Williams Robert, Sylvester Andrew Ekong, Inyang Anietie Akpabio, Okechukwu Ebuka Agbasi, and Joseph Onyeka Emegha. "Comparative Study of Dissipation Factor of Young and Mature Bitter Leaf (Vernonia Amygdalina) based on Location." BULETINUL INSTITUTULUI POLITEHNIC DIN IAȘI. Secția Matematica. Mecanică Teoretică. Fizică 70, no. 3-4 (2024): 7–17. https://doi.org/10.2478/bipmf-2024-0006.

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Abstract The use of Schering Bridge arrangement in combination with function generator and oscilloscope allows for precise measurement of heat dissipation in plant materials. This study aims to determine and compare the heat dissipation properties of young bitter leaf (YBL) and mature bitter leaf (MBL) samples obtained from two different locations. Measurements were conducted on ten fresh leaf samples per category from each location. Results indicated that for samples from the first location, the dissipation factors for YBL and MBL were (2.076 ± 0.010) and (3.231 ± 0.013) respectively, while f
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Okike, P.I., O.E. Edebiri, O.H. Blackie, et al. "Effect of Aqueous Extract of Vernonia Amygdalina (Bitter Leaf) and Ocimum Gratissimum (Scent Leaf) on the Blood Sugar Level Using Alloxan Diabetic Induced Wistar Rat." Research and Reviews: Journal of Forensic Nursing 3, no. 1 (2025): 23–30. https://doi.org/10.5281/zenodo.14777105.

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<em>This study aims to evaluate the antidiabetic potential of aqueous extracts of Vernonia amygdalina (bitter leaf) and Ocimum gratissimum (scent leaf) in alloxan-induced diabetic Wistar rats. </em><em>Twenty (20) adult Wistar rats were used for this study. For the first week all the wistar rats were left to acclimatize, given no drugs, only water and their feed. After acclimatization they were weighed, labelled with marks and their cages were also labelled. They were injected with alloxan so as to induce diabetes mellitus, thereby raising blood glucose level. The wistar rats were divided into
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I. Nwabueze, Rose. "ANALYSIS OF THE SECONDARY METABOLITES OF VERNONIA AMYGDALINA AND THE ANTIMICROBIAL EFFECTS ON PATHOGENIC ORGANISMS." International Journal of Advanced Research 10, no. 02 (2022): 686–92. http://dx.doi.org/10.21474/ijar01/14256.

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Medicinal plants have assumed the basis of traditional treatment and have proven effective in human health care worldwide. There is an increasing interest in complementary and alternative therapies to treat various illnesses. The continued consumption of antibiotics with little or no impact on bacteria has negatively affected health care delivery. Bitter leaf (Vernonia amygdalina) is a common shrub belonging to the family Asteraceae and a perennial shrub usually cultivated as a homestead vegetable and fodder tree in various parts of Nigeria. The current study examined the effect of bitter leaf
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Agu, Augustine, Patience Orji, Samuel Chime, Kelechi Duru, and Vivian Nwannadi. "Protective effect of aqueous leaf extract of vernonia amygdalina (bitter leaf) on gentamicin induced nephrotoxicity in adult wistar rat." Journal of Medical pharmaceutical and allied sciences 12, no. 3 (2023): 5790–95. http://dx.doi.org/10.55522/jmpas.v12i3.4662.

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This study evaluated the protective effect of an aqueous leaf extract of Vernonia amygdalina on the kidney functions and histological architecture of gentamicin-induced nephrotoxicity in adult Wistar rats. Twenty-five (25) Wistar rats were divided into five groups (groups 1–5), with five rats in each group. Group 1 served as the normal control. Group 2 was the negative control and received 40 mg/kg of body weight of gentamicin. Groups 3, 4, and 5 received an aqueous extract of V. amygdalina at doses of 100 mg, 200 mg, and 400 mg/kg of body weight, respectively, for 21 days. The gentamicin was
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Henrrietta Ogadimma Nnadi and Vincent Ugochukwu Igbokwe. "Evaluation of protective potentials of Vernonia amygdalina (Bitter leaf) extracts on some selected organs of cadmium chloride-induced hypertensive albino rats." World Journal of Advanced Research and Reviews 12, no. 3 (2021): 661–73. http://dx.doi.org/10.30574/wjarr.2021.12.3.0087.

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Many food components such as plant metabolites, have been shown to possess antioxidant activities. Vernonia amygdalina is widely consumed as vegetable for its rich array of phytochemicals and nutritional components which are attributed to its various health and medicinal benefits. The study evaluated the protective potentials of Vernonia amygdalina extracts on selected heart, Liver and Kidney of Cadmium Chloride-induced Hypertensive albino rats. The animals were induced hypertension with cadmium chloride. Total size of 50 albino rats of male and females were grouped according to sex in five di
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Ntesat, Unyeawaji Brownson, Okogbule-Wonodi Achinike, Chinwendu Emeka, and Mgbowaji Zacchaeus. "Novel Mathematical Models for Prediction of Spent Engine oil Interactions with Vernonia amygdalina Leaf Extract." Asian Journal of Advanced Research and Reports 18, no. 6 (2024): 214–25. http://dx.doi.org/10.9734/ajarr/2024/v18i6665.

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Aims: The michaelis-menten biokinetic model was used for the determination of key biokinetic parameters; maximum specific rate constant ( and michaelis-menten constant ( including the total polycyclic aromatic hydrocarbon (TPAH) degradation rate equation in the biostimulation of spent engine oil (SEO) – contaminated soil using vernonia amygdalina leaf (bitter leaf) extract. Study Design: Single factor experiment in a randomized complete block design (RCBD) was used in this study. Place and Duration of Study: Research farm, Rivers state university, Port-Harcourt, Nigeria, between April 2023 and
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Sa’idu, Haruna, Hassan Ismail Ahmad, Salau Abiola Olanrewaju, and Aisha Bello Mahmoud. "Antibacterial Effect of Bitter Leave (Vernonia amygdalina) on Klebsiella pneumoniae." Journal of Biochemistry, Microbiology and Biotechnology 8, no. 2 (2020): 16–20. http://dx.doi.org/10.54987/jobimb.v8i2.538.

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High rate of multi drug resistance bacteria caused by Klebsiella pneumoniae is increasing day by day, which in turn becomes medical and economic important to humans. The present study investigates the antibacterial effect of bitter leaf on Klebsiella pneumoniae. Frothing test and agar well diffusion method was used in this study. The former method indicate the presence of phytochemicals such saponin, tannin, flavonoid, alkaloid and carbohydrate in the plant while the later method indicate the potential effect of the plant extract to inhibit the growth of Klebsiella pneumoniae under different c
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Sailah, Illah, Trina E. Tallei, Linda Safitri, et al. "A network pharmacology approach to elucidate the anti-inflammatory and antioxidant effects of bitter leaf (Vernonia amygdalina Del.)." Narra J 4, no. 3 (2024): e1016. http://dx.doi.org/10.52225/narra.v4i3.1016.

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The therapeutic potential of bitter leaf (Vernonia amygdalina Del.) has been established both empirically and in various scientific investigations. However, the molecular pathways related to its possible anti-inflammatory and antioxidant properties remain unclear. Therefore, the aim of this study was to elucidate the molecular interactions between bitter leaf's bioactive compounds and cellular targets involved in these activities. The compounds in bitter leaf were identified using gas chromatography-mass spectrometry (GC-MS) analysis, and subsequently, a network pharmacology approach was emplo
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