Log in

Relevant bibliographies by topics / Vesicle fusion assays

Contents

Journal articles
Dissertations / Theses
Book chapters

Academic literature on the topic 'Vesicle fusion assays'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Vesicle fusion assays.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Vesicle fusion assays"

1

BLACKWOOD, R. Alexander, James E. SMOLEN, Ronald J. HESSLER, Donna M. HARSH, and Amy TRANSUE. "Development of an aqueous-space mixing assay for fusion of granules and plasma membranes from human neutrophils." Biochemical Journal 314, no. 2 (1996): 469–75. http://dx.doi.org/10.1042/bj3140469.

Full text

Abstract:

Several models have been developed to study neutrophil degranulation. At the most basic level, phospholipid vesicles have been used to investigate the lipid interactions occurring during membrane fusion. The two major forms of assays used to measure phospholipid vesicle fusion are based either on the dilution of tagged phospholipids within the membrane of the two fusing partners or the mixing of the aqueous contents of the vesicles. Although problems exist with both methods, the latter is considered to be more accurate and representative of true fusion. Using 8-aminonaphthalene-1,3,6-trisulpho

APA, Harvard, Vancouver, ISO, and other styles

2

Lai, Ying, and Yeon-Kyun Shin. "The importance of an asymmetric distribution of acidic lipids for synaptotagmin 1 function as a Ca2+ sensor." Biochemical Journal 443, no. 1 (2012): 223–29. http://dx.doi.org/10.1042/bj20112044.

Full text

Abstract:

Syt1 (synaptotagmin 1) is a major Ca2+ sensor for synaptic vesicle fusion. Although Syt1 is known to bind to SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) complexes and to the membrane, the mechanism by which Syt1 regulates vesicle fusion is controversial. In the present study we used in vitro lipid-mixing assays to investigate the Ca2+-dependent Syt1 function in proteoliposome fusion. To study the role of acidic lipids, the concentration of negatively charged DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine) in the vesicle was varied. Syt1 stimulated li

APA, Harvard, Vancouver, ISO, and other styles

3

Mukherjee, Indrani, and Charles Barlowe. "Overexpression of Sly41 suppresses COPII vesicle–tethering deficiencies by elevating intracellular calcium levels." Molecular Biology of the Cell 27, no. 10 (2016): 1635–49. http://dx.doi.org/10.1091/mbc.e15-10-0704.

Full text

Abstract:

SLY41 was identified as a multicopy suppressor of loss of Ypt1, a Rab GTPase essential for COPII vesicle tethering at the Golgi complex. SLY41 encodes a polytopic membrane protein with homology to a class of solute transporter proteins, but how overexpression suppresses vesicle-tethering deficiencies is not known. Here we show that Sly41 is efficiently packaged into COPII vesicles and actively cycles between the ER and Golgi compartments. SLY41 displays synthetic negative genetic interactions with PMR1, which encodes the major Golgi-localized Ca2+/Mn2+transporter and suggests that Sly41 influe

APA, Harvard, Vancouver, ISO, and other styles

4

de Bruijn, M. A. C., B. G. Goldhoorn, A. I. M. Zijlstra, G. N. J. Tytgat, and A. K. Groen. "Interaction of cholesterol-crystallization-promoting proteins with vesicles." Biochemical Journal 305, no. 1 (1995): 93–96. http://dx.doi.org/10.1042/bj3050093.

Full text

Abstract:

In this study, the interaction of mucin and concanavalin A-binding proteins isolated from human bile with cholesterol/phospholipid vesicles was investigated. Using resonance energy transfer assays originally developed by Struck, Hoekstra and Pagano [(1981) Biochemistry 20, 4093-4099], no significant protein-induced fusion or aggregation of vesicles was demonstrated. Instead of fusion, these proteins induced destabilization of cholesterol/phospholipid vesicles, as monitored by release of entrapped carboxyfluorescein. A good correlation (rho = 0.81) was obtained between the extent of leakage and

APA, Harvard, Vancouver, ISO, and other styles

5

Wang, Zhao, Huisheng Liu, Yiwen Gu, and Edwin R. Chapman. "Reconstituted synaptotagmin I mediates vesicle docking, priming, and fusion." Journal of Cell Biology 195, no. 7 (2011): 1159–70. http://dx.doi.org/10.1083/jcb.201104079.

Full text

Abstract:

The synaptic vesicle protein synaptotagmin I (syt) promotes exocytosis via its ability to penetrate membranes in response to binding Ca2+ and through direct interactions with SNARE proteins. However, studies using full-length (FL) membrane-embedded syt in reconstituted fusion assays have yielded conflicting results, including a lack of effect, or even inhibition of fusion, by Ca2+. In this paper, we show that reconstituted FL syt promoted rapid docking of vesicles (&lt;1 min) followed by a priming step (3–9 min) that was required for subsequent Ca2+-triggered fusion between v- and t-SNARE

APA, Harvard, Vancouver, ISO, and other styles

6

Jones, A. T., D. J. Spiro, T. Kirchhausen, P. Melancon, and M. Wessling-Resnick. "Studies on the inhibition of endosome fusion by GTPgammaS-bound ARF." Journal of Cell Science 112, no. 20 (1999): 3477–85. http://dx.doi.org/10.1242/jcs.112.20.3477.

Full text

Abstract:

Using a cell free assay, we have previously shown that ARF is not required for endosome fusion but that inhibition of fusion by GTPgammaS is dependent on a cytosolic pool of ARFs. Since ARF is proposed to function in intracellular membrane traffic by promoting vesicle biogenesis, and components of clathrin- and COP-coated vesicles have been localized on endosomal structures, we investigated whether ARF-mediated inhibition of early endosome fusion involves the recruitment or irreversible association of these proteins onto endosomal membranes. We now report that depletion of components of clathr

APA, Harvard, Vancouver, ISO, and other styles

7

Lou, Xiaochu, Jaewook Kim, Brenden J. Hawk та Yeon-Kyun Shin. "α-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking". Biochemical Journal 474, № 12 (2017): 2039–49. http://dx.doi.org/10.1042/bcj20170200.

Full text

Abstract:

Misfolded α-synuclein (A-syn) is widely recognized as the primal cause of neurodegenerative diseases including Parkinson's disease and dementia with Lewy bodies. The normal cellular function of A-syn has, however, been elusive. There is evidence that A-syn plays multiple roles in the exocytotic pathway in the neuron, but the underlying molecular mechanisms are unclear. A-syn has been known to interact with negatively charged phospholipids and with vesicle SNARE protein VAMP2. Using single-vesicle docking/fusion assays, we find that A-syn promotes SNARE-dependent vesicles docking significantly

APA, Harvard, Vancouver, ISO, and other styles

8

Nguyen, Trinh T., and David T. Cramb. "Elucidation of the mechanism and energy barrier for anesthetic triggered membrane fusion in model membranes." Canadian Journal of Chemistry 97, no. 6 (2019): 474–82. http://dx.doi.org/10.1139/cjc-2018-0405.

Full text

Abstract:

Membrane fusion is vital for cellular function and is generally mediated via fusogenic proteins and peptides. The mechanistic details and subsequently the transition state dynamics of membrane fusion will be dependent on the type of the fusogenic agent. We have previously established the potential of general anesthetics as a new class of fusion triggering agents in model membranes. We employed two-photon excitation fluorescence cross-correlation spectroscopy (TPE-FCCS) to report on vesicle association kinetics and steady-state fluorescence dequenching assays to monitor lipid mixing kinetics. U

APA, Harvard, Vancouver, ISO, and other styles

9

Happe, Scott, and Peggy Weidman. "Cell-free Transport to Distinct Golgi Cisternae Is Compartment Specific and ARF Independent." Journal of Cell Biology 140, no. 3 (1998): 511–23. http://dx.doi.org/10.1083/jcb.140.3.511.

Full text

Abstract:

The small GTPase ADP-ribosylation factor (ARF) is absolutely required for coatomer vesicle formation on Golgi membranes but not for anterograde transport to the medial-Golgi in a mammalian in vitro transport system. This might indicate that the in vivo mechanism of intra-Golgi transport is not faithfully reproduced in vitro, or that intra-Golgi transport occurs by a nonvesicular mechanism. As one approach to distinguishing between these possibilities, we have characterized two additional cell-free systems that reconstitute transport to the trans-Golgi (trans assay) and trans-Golgi network (TGN

APA, Harvard, Vancouver, ISO, and other styles

10

Lee, Jihye, and J. Troy Littleton. "Transmembrane tethering of synaptotagmin to synaptic vesicles controls multiple modes of neurotransmitter release." Proceedings of the National Academy of Sciences 112, no. 12 (2015): 3793–98. http://dx.doi.org/10.1073/pnas.1420312112.

Full text

Abstract:

Synaptotagmin 1 (Syt1) is a synaptic vesicle integral membrane protein that regulates neurotransmitter release by activating fast synchronous fusion and suppressing slower asynchronous release. The cytoplasmic C2 domains of Syt1 interact with SNAREs and plasma membrane phospholipids in a Ca2+-dependent manner and can substitute for full-length Syt1 in in vitro membrane fusion assays. To determine whether synaptic vesicle tethering of Syt1 is required for normal fusion in vivo, we performed a structure-function study with tethering mutants at the Drosophila larval neuromuscular junction. Transg

APA, Harvard, Vancouver, ISO, and other styles

Dissertations / Theses on the topic "Vesicle fusion assays"

1

Groth, Mike Christopher. "Investigation of the Linker Region of Coiled Coil SNARE-Analoga and Membrane Composition on Vesicle Fusion." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://hdl.handle.net/21.11130/00-1735-0000-0005-159B-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Schirmacher, Anastasiya. "Modification of transmembrane peptides to probe SNARE-induced membrane fusion and cross-presentation of membrane-buried epitopes." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://hdl.handle.net/21.11130/00-1735-0000-0005-1576-F.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Schwamborn, Miriam. "Establishment of a fluorescence assay for characterization of protein-mediated vesicle fusion and acidification." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3E83-7.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Hurtley, S. M. "Reconstitution of an endocytic fusion event in a cell-free system." Thesis, Open University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484412.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Witkowska, Agata [Verfasser], Reinhard [Akademischer Betreuer] Jahn, Reinhard [Gutachter] Jahn, and Andreas [Gutachter] Janshoff. "Study of SNARE-mediated membrane fusion with a novel single vesicle fusion assay / Agata Witkowska ; Gutachter: Reinhard Jahn, Andreas Janshoff ; Betreuer: Reinhard Jahn." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1161183191/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Schwamborn, Miriam [Verfasser], Claudia [Akademischer Betreuer] [Gutachter] Steinem, and Mikael [Gutachter] Simons. "Establishment of a fluorescence assay for characterization of protein-mediated vesicle fusion and acidification / Miriam Schwamborn ; Gutachter: Claudia Steinem, Mikael Simons ; Betreuer: Claudia Steinem." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://d-nb.info/1135778361/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Chraïbi, Zakaria. "Transport intracellulaire du proteolipide de folch-pi dans l'oligodendrocyte, role des vesicules mantelees dans le processus de myelinisation." Paris 6, 1987. http://www.theses.fr/1987PA066067.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Schwenen, Lando Lantbert Gregor. "Untersuchung einzelner SNARE-vermittelter Membranfusionsereignisse auf planaren porenüberspannenden Membranen." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://hdl.handle.net/11858/00-1735-0000-0023-9650-F.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Witkowska, Agata. "Study of SNARE-mediated membrane fusion with a novel single vesicle fusion assay." Doctoral thesis, 2016. http://hdl.handle.net/11858/00-1735-0000-002E-E41C-7.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Vesicle fusion assays"

1

Kreye, Susanne, Jörg Malsam, and Thomas H. Söllner. "In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion." In Methods in Molecular Biology. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-178-9_3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Sung, Mi Sook, and Tae-Young Yoon. "Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle–Vesicle Fusion Assay." In Methods in Molecular Biology. Springer US, 2019. http://dx.doi.org/10.1007/978-1-0716-0167-9_13.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Kim, Jae-Yeol, Bong-Kyu Choi, Yeon-Kyun Shin, and Nam Ki Lee. "Solution Single-Vesicle Fusion Assay by Single-Molecule Alternating-Laser Excitation." In Neuromethods. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-676-4_1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Hiebsch, R. R., and B. W. Wattenberg. "Fusion Rapidly Follows Vesicle Transport to the Target Membrane in Protein Transport through the Golgi Apparatus in Vitro. A Re-evaluation of Transport Kinetics Based on the Finding that the Glycosylation Used to Mark Transport, and Not Transport Itself, is Rate Limiting in the Assay." In Molecular Mechanisms of Membrane Traffic. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-662-02928-2_4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Braell, William A. "[3] Detection of endocytic vesicle fusion in Vitro, using assay based on avidin-biotin association reaction." In Reconstitution of Intracellular Transport. Elsevier, 1992. http://dx.doi.org/10.1016/0076-6879(92)19005-q.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!