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Journal articles on the topic 'Vibrio. luxR'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Bazhenov, Sergey, Olga Melkina, Vadim Fomin, Ekaterina Scheglova, Pavel Krasnik, Svetlana Khrulnova, Gennadii Zavilgelsky, and Ilya Manukhov. "LitR directly upregulates autoinducer synthesis and luminescence in Aliivibrio logei." PeerJ 9 (September 21, 2021): e12030. http://dx.doi.org/10.7717/peerj.12030.

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LitR is a master-regulator of transcription in the ainS/R and luxS/PQ quorum sensing (QS) systems of bacteria from Vibrio and Aliivibrio genera. Here, we for the first time directly investigated the influence of LitR on gene expression in the luxI/R QS system of psychrophilic bacteria Aliivibrio logei. Investigated promoters were fused with Photorhabdus luminescens luxCDABE reporter genes cassette in a heterological system of Escherichia coli cells, litR A. logei was introduced into the cells under control of Plac promoter. LitR has been shown to upregulate genes of autoinducer synthase (luxI)

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Antunes, Luis Caetano M., Rosana B. R. Ferreira, C. Phoebe Lostroh, and E. Peter Greenberg. "A Mutational Analysis Defines Vibrio fischeri LuxR Binding Sites." Journal of Bacteriology 190, no. 13 (December 14, 2007): 4392–97. http://dx.doi.org/10.1128/jb.01443-07.

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ABSTRACT Vibrio fischeri quorum sensing involves the LuxI and LuxR proteins. The LuxI protein generates the quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL), and LuxR is a signal-responsive transcriptional regulator which activates the luminescence (lux) genes and 17 other V. fischeri genes. For activation of the lux genes, LuxR binds to a 20-base-pair inverted repeat, the lux box, which is centered 42.5 base pairs upstream of the transcriptional start of the lux operon. Similar lux box-like elements have been identified in only a few of the LuxR-activated V. fischeri prom

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3

Urbanowski, M. L., C. P. Lostroh, and E. P. Greenberg. "Reversible Acyl-Homoserine Lactone Binding to Purified Vibrio fischeri LuxR Protein." Journal of Bacteriology 186, no. 3 (February 1, 2004): 631–37. http://dx.doi.org/10.1128/jb.186.3.631-637.2004.

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ABSTRACT The Vibrio fischeri LuxR protein is the founding member of a family of acyl-homoserine lactone-responsive quorum-sensing transcription factors. Previous genetic evidence indicates that in the presence of its quorum-sensing signal, N-(3-oxohexanoyl) homoserine lactone (3OC6-HSL), LuxR binds to lux box DNA within the promoter region of the luxI gene and activates transcription of the luxICDABEG luminescence operon. We have purified LuxR from recombinant Escherichia coli. Purified LuxR binds specifically and with high affinity to DNA containing a lux box. This binding requires addition o

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4

Egland, Kristi A., and E. P. Greenberg. "Conversion of the Vibrio fischeriTranscriptional Activator, LuxR, to a Repressor." Journal of Bacteriology 182, no. 3 (February 1, 2000): 805–11. http://dx.doi.org/10.1128/jb.182.3.805-811.2000.

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ABSTRACT The Vibrio fischeri luminescence (lux) operon is regulated by a quorum-sensing system that involves the transcriptional activator (LuxR) and an acyl-homoserine lactone signal. Transcriptional activation requires the presence of a 20-base inverted repeat termed the lux box at a position centered 42.5 bases upstream of the transcriptional start of the lux operon. LuxR has proven difficult to study in vitro. A truncated form of LuxR has been purified, and together with ς70 RNA polymerase it can activate transcription of the lux operon. Both the truncated LuxR and RNA polymerase are requi

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5

Antunes, Luis Caetano M., Amy L. Schaefer, Rosana B. R. Ferreira, Nan Qin, Ann M. Stevens, Edward G. Ruby, and E. Peter Greenberg. "Transcriptome Analysis of the Vibrio fischeri LuxR-LuxI Regulon." Journal of Bacteriology 189, no. 22 (September 7, 2007): 8387–91. http://dx.doi.org/10.1128/jb.00736-07.

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ABSTRACT The Vibrio fischeri quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL) activates expression of the seven-gene luminescence operon. We used microarrays to unveil 18 additional 3OC6-HSL-controlled genes, 3 of which had been identified by other means previously. We show most of these genes are regulated by the 3OC6-HSL-responsive transcriptional regulator LuxR directly. This demonstrates that V. fischeri quorum sensing regulates a substantial number of genes other than those involved in light production.

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Zhang, Jun, Bing Liu, Dan Gu, Yuan Hao, Mo Chen, Yue Ma, Xiaohui Zhou, David Reverter, Yuanxing Zhang, and Qiyao Wang. "Binding site profiles and N-terminal minor groove interactions of the master quorum-sensing regulator LuxR enable flexible control of gene activation and repression." Nucleic Acids Research 49, no. 6 (March 8, 2021): 3274–93. http://dx.doi.org/10.1093/nar/gkab150.

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Abstract LuxR is a TetR family master quorum sensing (QS) regulator activating or repressing expression of hundreds of genes that control collective behaviors in Vibrios with underlying mechanism unknown. To illuminate how this regulator controls expression of various target genes, we applied ChIP-seq and DNase I-seq technologies. Vibrio alginolyticus LuxR controls expression of ∼280 genes that contain either symmetric palindrome (repDNA) or asymmetric (actDNA) binding motifs with different binding profiles. The median number of LuxR binding sites for activated genes are nearly double for that

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7

Callahan, Sean M., and Paul V. Dunlap. "LuxR- and Acyl-Homoserine-Lactone-Controlled Non-luxGenes Define a Quorum-Sensing Regulon in Vibrio fischeri." Journal of Bacteriology 182, no. 10 (May 15, 2000): 2811–22. http://dx.doi.org/10.1128/jb.182.10.2811-2822.2000.

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ABSTRACT The luminescence (lux) operon (luxICDABEG) of the symbiotic bacterium Vibrio fischeri is regulated by the transcriptional activator LuxR and two acyl-homoserine lactone (acyl-HSL) autoinducers (the luxI-dependent 3-oxo-hexanoyl-HSL [3-oxo-C6-HSL] and the ainS-dependent octanoyl-HSL [C8-HSL]) in a population density-responsive manner called quorum sensing. To identify quorum-sensing-regulated (QSR) proteins different from those encoded by lux genes, we examined the protein patterns of V. fischeri quorum-sensing mutants defective in luxI, ainS, andluxR by two-dimensional polyacrylamide

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8

Qin, Nan, Sean M. Callahan, Paul V. Dunlap, and Ann M. Stevens. "Analysis of LuxR Regulon Gene Expression during Quorum Sensing in Vibrio fischeri." Journal of Bacteriology 189, no. 11 (March 30, 2007): 4127–34. http://dx.doi.org/10.1128/jb.01779-06.

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ABSTRACT The regulation of the lux operon (luxICDABEG) of Vibrio fischeri has been intensively studied as a model for quorum sensing in proteobacteria. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis previously identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on LuxR and 3-oxo-hexanoyl-l-homoserine lactone (3-oxo-C6-HSL). To determine if the LuxR-dependent regulation of the genes encoding these proteins was due to direct transcriptional control by LuxR and 3-oxo-C6-HSL or instead was due to indirect control via an unide

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McDougald, Diane, Scott A. Rice, and Staffan Kjelleberg. "SmcR-Dependent Regulation of Adaptive Phenotypes inVibrio vulnificus." Journal of Bacteriology 183, no. 2 (January 15, 2001): 758–62. http://dx.doi.org/10.1128/jb.183.2.758-762.2001.

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ABSTRACT Vibrio vulnificus contains homologues of the V. harveyi luxR and luxS genes. A null mutation insmcR (luxR) resulted in a defect in starvation survival, inhibition of starvation-induced maintenance of culturability that occurs when V. vulnificusis starved prior to low-temperature incubation, and increased expression of stationary-phase phenotypes.

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Miyamoto, Carol M., Paul V. Dunlap, Edward G. Ruby, and Edward A. Meighen. "LuxO controls luxR expression in Vibrio harveyi: evidence for a common regulatory mechanism in Vibrio." Molecular Microbiology 48, no. 2 (April 4, 2003): 537–48. http://dx.doi.org/10.1046/j.1365-2958.2003.03453.x.

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11

McCarter, Linda L. "OpaR, a Homolog of Vibrio harveyi LuxR, Controls Opacity of Vibrio parahaemolyticus." Journal of Bacteriology 180, no. 12 (June 15, 1998): 3166–73. http://dx.doi.org/10.1128/jb.180.12.3166-3173.1998.

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ABSTRACT Vibrio parahaemolyticus is an organism well adapted to communal life on surfaces. When grown on a surface or in a viscous layer, the bacterium induces a large gene system and differentiates to swarmer cells capable of movement over and colonization of surfaces.V. parahaemolyticus displays additional phenotypic versatility manifested as variable colony morphology, switching between translucent and opaque colony types. Although not itself luminescent,V. parahaemolyticus produces autoinducer molecules capable of inducing luminescence in Vibrio harveyi. To examine the role of quorum signa

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12

Trott, Amy E., and Ann M. Stevens. "Amino Acid Residues in LuxR Critical for Its Mechanism of Transcriptional Activation during Quorum Sensing inVibrio fischeri." Journal of Bacteriology 183, no. 1 (January 1, 2001): 387–92. http://dx.doi.org/10.1128/jb.183.1.387-392.2001.

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ABSTRACT PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the variant forms of LuxR to activate transcription of the lux operon was examined by using in vivo assays in recombinant Escherichia coli. Eight recombinant strains produced luciferase at levels less than 50% of that of a strain expressing wild-type LuxR. Western immunoblotti

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13

Stevens, Ann M., Nobuyuki Fujita, Akira Ishihama та E. P. Greenberg. "Involvement of the RNA Polymerase α-Subunit C-Terminal Domain in LuxR-Dependent Activation of the Vibrio fischeri Luminescence Genes". Journal of Bacteriology 181, № 15 (1 серпня 1999): 4704–7. http://dx.doi.org/10.1128/jb.181.15.4704-4707.1999.

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ABSTRACT LuxR is a ς70 RNA polymerase (RNAP)-dependent transcriptional activator that controls expression of the Vibrio fischeri lux operon in response to an acylhomoserine lactone-cell density signal. We have investigated whether the α-subunit C-terminal domain (αCTD) of RNAP is required for LuxR activity. A purified signal-independent, LuxR C-terminal domain-containing polypeptide (LuxRΔN) was used to study the activation of transcription from theluxI promoter in vitro. Initiation of luxoperon transcription was observed in the presence of LuxRΔN and wild-type RNAP but not in the presence of

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14

Shao, Chung-Ping, and Lien-I. Hor. "Regulation of Metalloprotease Gene Expression in Vibrio vulnificus by a Vibrio harveyi LuxR Homologue." Journal of Bacteriology 183, no. 4 (February 15, 2001): 1369–75. http://dx.doi.org/10.1128/jb.183.4.1369-1375.2001.

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ABSTRACT Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyiLuxR-like transcriptional activator. To find out whether a LuxR homologue exists in V. vulnificus, a gene library of this organism was screened by colony hybridization using a probe derived from a sequence that is conserved in various luxR-like genes of vibrios. A gene containing a 618-bp open reading

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15

Hawkins, Andrew C., Frances H. Arnold, Rainer Stuermer, Bernhard Hauer, and Jared R. Leadbetter. "Directed Evolution of Vibrio fischeri LuxR for Improved Response to Butanoyl-Homoserine Lactone." Applied and Environmental Microbiology 73, no. 18 (August 3, 2007): 5775–81. http://dx.doi.org/10.1128/aem.00060-07.

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ABSTRACT LuxR is the 3-oxohexanoyl-homoserine lactone (3OC6HSL)-dependent transcriptional activator of the prototypical acyl-homoserine lactone (AHL) quorum-sensing system of Vibrio fischeri. Wild-type LuxR exhibits no response to butanoyl-HSL (C4HSL) in quantitative bioassays at concentrations of up to 1 μM; a previously described LuxR variant (LuxR-G2E) exhibits a broadened response to diverse AHLs, including pentanoyl-HSL (C5HSL), but not to C4HSL. Here, two rounds of directed evolution of LuxR-G2E generated variants of LuxR that responded to C4HSL at concentrations as low as 10 nM. One var

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16

O'Grady, Elizabeth A., and Charles F. Wimpee. "Mutations in the lux Operon of Natural Dark Mutants in the Genus Vibrio." Applied and Environmental Microbiology 74, no. 1 (November 2, 2007): 61–66. http://dx.doi.org/10.1128/aem.01199-07.

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ABSTRACT Bacterial bioluminescence can display a wide range of intensities among strains, from very bright to undetectable, and it has been shown previously that there are nonluminous vibrios that possess lux genes. In this paper, we report the isolation and characterization of completely dark natural mutants in the genus Vibrio. Screening of over 600 Vibrio isolates with a luxA gene probe revealed that approximately 5% carried the luxA gene. Bioluminescence assays of the luxA-positive isolates, followed by repetitive extragenic palindromic-PCR fingerprinting, showed three unique genotypes tha

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Perry, Lynda L., Nathan G. Bright, Richard J. Carroll, Jr., M. Cathy Scott, Michael S. Allen, and Bruce M. Applegate. "Molecular characterization of autoinduction of bioluminescence in the Microtox® indicator strain Vibrio fischeri ATCC 49387." Canadian Journal of Microbiology 51, no. 7 (July 1, 2005): 549–57. http://dx.doi.org/10.1139/w05-019.

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Repeated attempts to clone the luxI from Vibrio fischeri ATCC 49387 failed to produce a clone carrying a functional LuxI. Sequence data from the clones revealed the presence of a polymorphism when compared with previously published luxI sequences, prompting further characterization of bioluminescence regulation in V. fischeri ATCC 49387. Further investigation of V. fischeri ATCC 49387 revealed that its LuxI protein lacks detectable LuxI activity due to the presence of a glutamine residue at position 125 in the deduced amino acid sequence. Specific bioluminescence in V. fischeri ATCC 49387 incr

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Williams, Joshua W., A. L. Ritter, and Ann M. Stevens. "CsrA modulates luxR transcript levels in Vibrio fischeri." FEMS Microbiology Letters 329, no. 1 (February 8, 2012): 28–35. http://dx.doi.org/10.1111/j.1574-6968.2012.02499.x.

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Kimura, Yuki, Yohei Tashiro, Kyoichi Saito, Shigeko Kawai-Noma, and Daisuke Umeno. "Directed evolution of Vibrio fischeri LuxR signal sensitivity." Journal of Bioscience and Bioengineering 122, no. 5 (November 2016): 533–38. http://dx.doi.org/10.1016/j.jbiosc.2016.04.010.

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20

Plener, Laure, Nicola Lorenz, Matthias Reiger, Tiago Ramalho, Ulrich Gerland, and Kirsten Jung. "The Phosphorylation Flow of the Vibrio harveyi Quorum-Sensing Cascade Determines Levels of Phenotypic Heterogeneity in the Population." Journal of Bacteriology 197, no. 10 (March 9, 2015): 1747–56. http://dx.doi.org/10.1128/jb.02544-14.

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ABSTRACTQuorum sensing (QS) is a communication process that enables a bacterial population to coordinate and synchronize specific behaviors. The bioluminescent marine bacteriumVibrio harveyiintegrates three autoinducer (AI) signals into one quorum-sensing cascade comprising a phosphorelay involving three hybrid sensor kinases: LuxU; LuxO, an Hfq/small RNA (sRNA) switch; and the transcriptional regulator LuxR. Using a new set ofV. harveyimutants lacking genes for the AI synthases and/or sensors, we assayed the activity of the quorum-sensing cascade at the population and single-cell levels, with

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Nelson, Eric J., Hege S. Tunsjø, Pat M. Fidopiastis, Henning Sørum, and Edward G. Ruby. "A Novel lux Operon in the Cryptically Bioluminescent Fish Pathogen Vibrio salmonicida Is Associated with Virulence." Applied and Environmental Microbiology 73, no. 6 (February 2, 2007): 1825–33. http://dx.doi.org/10.1128/aem.02255-06.

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ABSTRACT The cold-water-fish pathogen Vibrio salmonicida expresses a functional bacterial luciferase but produces insufficient levels of its aliphatic-aldehyde substrate to be detectably luminous in culture. Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular characterization of the lux operon and (ii) test whether the bioluminescence gene cluster is associated with virulence. Cloning and sequencing of the V. salmonicida lux operon revealed that homologs of all of the genes required for luminescence are present: luxAB (luciferase) and luxCDE (aliphatic

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Chatterjee, Jaidip, Carol M. Miyamoto, and Edward A. Meighen. "Autoregulation of luxR: the Vibrio harveyi lux-operon activator functions as a repressor." Molecular Microbiology 20, no. 2 (April 1996): 415–25. http://dx.doi.org/10.1111/j.1365-2958.1996.tb02628.x.

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23

Finney, Angela H., Robert J. Blick, Katsuhiko Murakami, Akira Ishihama, and Ann M. Stevens. "Role of the C-Terminal Domain of the Alpha Subunit of RNA Polymerase in LuxR-Dependent Transcriptional Activation of the lux Operon during Quorum Sensing." Journal of Bacteriology 184, no. 16 (August 15, 2002): 4520–28. http://dx.doi.org/10.1128/jb.184.16.4520-4528.2002.

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ABSTRACT During quorum sensing in Vibrio fischeri, the luminescence, or lux, operon is regulated in a cell density-dependent manner by the activator LuxR in the presence of an acylated homoserine lactone autoinducer molecule [N-(3-oxohexanoyl) homoserine lactone]. LuxR, which binds to the lux operon promoter at a position centered at −42.5 relative to the transcription initiation site, is thought to function as an ambidextrous activator making multiple contacts with RNA polymerase (RNAP). The specific role of the α-subunit C-terminal domain (αCTD) of RNAP in LuxR-dependent transcriptional acti

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24

Egland, Kristi A., and E. P. Greenberg. "Quorum Sensing in Vibrio fischeri: Analysis of the LuxR DNA Binding Region by Alanine-Scanning Mutagenesis." Journal of Bacteriology 183, no. 1 (January 1, 2001): 382–86. http://dx.doi.org/10.1128/jb.183.1.382-386.2001.

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ABSTRACT LuxR is the transcriptional activator for quorum-sensing control of luminescence in Vibrio fischeri. A series of alanine-scanning mutants spanning a predicted helix-turn-helix region in the DNA binding domain of LuxR was constructed, and the activity of each of the LuxR mutant proteins in recombinant Escherichia coli was investigated. The region covered by the mutagenesis spanned residues 190 to 224. About half of the alanine-scanning mutants showed activities similar to that of the wild-type LuxR: at least two were positive-control mutants, four appeared to be defective in DNA bindin

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Rutherford, Steven T., Julie S. Valastyan, Thibaud Taillefumier, Ned S. Wingreen, and Bonnie L. Bassler. "Comprehensive analysis reveals how single nucleotides contribute to noncoding RNA function in bacterial quorum sensing." Proceedings of the National Academy of Sciences 112, no. 44 (October 19, 2015): E6038—E6047. http://dx.doi.org/10.1073/pnas.1518958112.

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Five homologous noncoding small RNAs (sRNAs), called the Qrr1-5 sRNAs, function in the Vibrio harveyi quorum-sensing cascade to drive its operation. Qrr1-5 use four different regulatory mechanisms to control the expression of ∼20 mRNA targets. Little is known about the roles individual nucleotides play in mRNA target selection, in determining regulatory mechanism, or in defining Qrr potency and dynamics of target regulation. To identify the nucleotides vital for Qrr function, we developed a method we call RSort-Seq that combines saturating mutagenesis, fluorescence-activated cell sorting, high

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White, Catharine E., and Stephen C. Winans. "Cell–cell communication in the plant pathogen Agrobacterium tumefaciens." Philosophical Transactions of the Royal Society B: Biological Sciences 362, no. 1483 (March 13, 2007): 1135–48. http://dx.doi.org/10.1098/rstb.2007.2040.

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The plant pathogen Agrobacterium tumefaciens induces the formation of crown gall tumours at wound sites on host plants by directly transforming plant cells. This disease strategy benefits the bacteria as the infected plant tissue produces novel nutrients, called opines, that the colonizing bacteria can use as nutrients. Almost all of the genes that are required for virulence, and all of the opine uptake and utilization genes, are carried on large tumour-inducing (Ti) plasmids. The observation more than 25 years ago that specific opines are required for Ti plasmid conjugal transfer led to the d

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Newman, Jane D., Meghan M. Russell, Lixin Fan, Yun-Xing Wang, Giovanni Gonzalez-Gutierrez, and Julia C. van Kessel. "The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences." Nucleic Acids Research 49, no. 10 (May 22, 2021): 5967–84. http://dx.doi.org/10.1093/nar/gkab387.

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Abstract Quorum sensing gene expression in vibrios is regulated by the LuxR/HapR family of transcriptional factors, which includes Vibrio vulnificus SmcR. The consensus binding site of Vibrio LuxR/HapR/SmcR proteins is palindromic but highly degenerate with sequence variations at each promoter. To examine the mechanism by which SmcR recognizes diverse DNA sites, we generated SmcR separation-of-function mutants that either repress or activate transcription but not both. SmcR N55I is restricted in recognition of single base-pair variations in DNA binding site sequences and thus is defective at t

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28

Lu, Yang. "Engineering Vibrio fischeri transcriptional activator LuxR for diverse transcriptional activities." Biotechnology Letters 38, no. 9 (June 3, 2016): 1459–63. http://dx.doi.org/10.1007/s10529-016-2134-z.

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Swartzman, E., M. Silverman, and E. A. Meighen. "The luxR gene product of Vibrio harveyi is a transcriptional activator of the lux promoter." Journal of Bacteriology 174, no. 22 (1992): 7490–93. http://dx.doi.org/10.1128/jb.174.22.7490-7493.1992.

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Shadel, G. S., and T. O. Baldwin. "Positive autoregulation of the Vibrio fischeri luxR gene. LuxR and autoinducer activate cAMP-catabolite gene activator protein complex-independent and -dependent luxR transcription." Journal of Biological Chemistry 267, no. 11 (April 1992): 7696–702. http://dx.doi.org/10.1016/s0021-9258(18)42571-9.

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Croxatto, Antony, Victoria J. Chalker, Johan Lauritz, Jana Jass, Andrea Hardman, Paul Williams, Miguel Cámara, and Debra L. Milton. "VanT, a Homologue of Vibrio harveyi LuxR, Regulates Serine, Metalloprotease, Pigment, and Biofilm Production in Vibrio anguillarum." Journal of Bacteriology 184, no. 6 (March 15, 2002): 1617–29. http://dx.doi.org/10.1128/jb.184.6.1617-1629.2002.

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ABSTRACT Vibrio anguillarum possesses at least two N-acylhomoserine lactone (AHL) quorum-sensing circuits, one of which is related to the luxMN system of Vibrio harveyi. In this study, we have cloned an additional gene of this circuit, vanT, encoding a V. harveyi LuxR-like transcriptional regulator. A V. anguillarum ΔvanT null mutation resulted in a significant decrease in total protease activity due to loss of expression of the metalloprotease EmpA, but no changes in either AHL production or virulence. Additional genes positively regulated by VanT were identified from a plasmid-based gene lib

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van Kessel, Julia C., Steven T. Rutherford, Jian-Ping Cong, Sofia Quinodoz, James Healy, and Bonnie L. Bassler. "Quorum Sensing Regulates the Osmotic Stress Response in Vibrio harveyi." Journal of Bacteriology 197, no. 1 (October 13, 2014): 73–80. http://dx.doi.org/10.1128/jb.02246-14.

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Bacteria use a chemical communication process called quorum sensing to monitor cell density and to alter behavior in response to fluctuations in population numbers. Previous studies withVibrio harveyihave shown that LuxR, the master quorum-sensing regulator, activates and represses >600 genes. These include six genes that encode homologs of theEscherichia coliBet and ProU systems for synthesis and transport, respectively, of glycine betaine, an osmoprotectant used during osmotic stress. Here we show that LuxR activates expression of the glycine betaine operonbetIBA-proXWV, which enhances gr

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Miyamoto, Carol M., Weiqun Sun, and Edward A. Meighen. "The LuxR regulator protein controls synthesis of polyhydroxybutyrate in Vibrio harveyi." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1384, no. 2 (May 1998): 356–64. http://dx.doi.org/10.1016/s0167-4838(98)00028-4.

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Miyamoto, Carol M., Eric E. Smith, Eiana Swartzman, Jie‐Gang Cao, Angus F. Graham, and Edward A. Meighen. "Proximal and distal sites bind LuxR independently and activate expression of the Vibrio harveyi lux operon." Molecular Microbiology 14, no. 2 (October 1994): 255–62. http://dx.doi.org/10.1111/j.1365-2958.1994.tb01286.x.

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35

Miyamoto, Carol M., Jaidip Chatterjee, Elana Swartzman, Rose Szittner, and Edward A. Meighen. "The role of the lux autoinducer in regulating luminescence in Vibrio harveyi; control of luxR expression." Molecular Microbiology 19, no. 4 (February 1996): 767–75. http://dx.doi.org/10.1046/j.1365-2958.1996.417948.x.

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36

Freeman, Jeremy A., and Bonnie L. Bassler. "Sequence and Function of LuxU: a Two-Component Phosphorelay Protein That Regulates Quorum Sensing inVibrio harveyi." Journal of Bacteriology 181, no. 3 (February 1, 1999): 899–906. http://dx.doi.org/10.1128/jb.181.3.899-906.1999.

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ABSTRACT Vibrio harveyi regulates the expression of bioluminescence (lux) in response to cell density, a phenomenon known as quorum sensing. In V. harveyi, two independent quorum-sensing systems exist, and each produces, detects, and responds to a specific cell density-dependent autoinducer signal. The autoinducers are recognized by two-component hybrid sensor kinases called LuxN and LuxQ, and sensory information from both systems is transduced by a phosphorelay mechanism to the response regulator protein LuxO. Genetic evidence suggests that LuxO-phosphate negatively regulates the expression o

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Dunlap, P. V., and E. P. Greenberg. "Control of Vibrio fischeri lux gene transcription by a cyclic AMP receptor protein-luxR protein regulatory circuit." Journal of Bacteriology 170, no. 9 (1988): 4040–46. http://dx.doi.org/10.1128/jb.170.9.4040-4046.1988.

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Waters, Christopher M., Julie T. Wu, Meghan E. Ramsey, Rebecca C. Harris, and Bonnie L. Bassler. "Control of the Type 3 Secretion System in Vibrio harveyi by Quorum Sensing through Repression of ExsA." Applied and Environmental Microbiology 76, no. 15 (June 11, 2010): 4996–5004. http://dx.doi.org/10.1128/aem.00886-10.

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ABSTRACT The type 3 secretion system (T3SS) genes of Vibrio harveyi are activated at low cell density and repressed at high cell density by quorum sensing (QS). Repression requires LuxR, the master transcriptional regulator of QS-controlled genes. Here, we determine the mechanism underlying the LuxR repression of the T3SS system. Using a fluorescence-based cell sorting approach, we isolated V. harveyi mutants that are unable to express T3SS genes at low cell density and identified two mutations in the V. harveyi exsBA operon. While LuxR directly represses the expression of exsBA, complementati

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Schaefer, A. L., B. L. Hanzelka, A. Eberhard, and E. P. Greenberg. "Quorum sensing in Vibrio fischeri: probing autoinducer-LuxR interactions with autoinducer analogs." Journal of bacteriology 178, no. 10 (1996): 2897–901. http://dx.doi.org/10.1128/jb.178.10.2897-2901.1996.

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Colton, Deanna M., Eric V. Stabb, and Stephen J. Hagen. "Modeling Analysis of Signal Sensitivity and Specificity by Vibrio fischeri LuxR Variants." PLOS ONE 10, no. 5 (May 11, 2015): e0126474. http://dx.doi.org/10.1371/journal.pone.0126474.

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Kolibachuk, D., and E. P. Greenberg. "The Vibrio fischeri luminescence gene activator LuxR is a membrane-associated protein." Journal of Bacteriology 175, no. 22 (1993): 7307–12. http://dx.doi.org/10.1128/jb.175.22.7307-7312.1993.

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Slock, J., D. VanRiet, D. Kolibachuk, and E. P. Greenberg. "Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis." Journal of Bacteriology 172, no. 7 (1990): 3974–79. http://dx.doi.org/10.1128/jb.172.7.3974-3979.1990.

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Tashiro, Yohei, Yuki Kimura, Maiko Furubayashi, Akira Tanaka, Kei Terakubo, Kyoichi Saito, Shigeko Kawai-Noma, and Daisuke Umeno. "Directed evolution of the autoinducer selectivity of Vibrio fischeri LuxR." Journal of General and Applied Microbiology 62, no. 5 (2016): 240–47. http://dx.doi.org/10.2323/jgam.2016.04.005.

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Choi, S. H., and E. P. Greenberg. "The C-terminal region of the Vibrio fischeri LuxR protein contains an inducer-independent lux gene activating domain." Proceedings of the National Academy of Sciences 88, no. 24 (December 15, 1991): 11115–19. http://dx.doi.org/10.1073/pnas.88.24.11115.

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Stevens, A. M., K. M. Dolan, and E. P. Greenberg. "Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region." Proceedings of the National Academy of Sciences 91, no. 26 (December 20, 1994): 12619–23. http://dx.doi.org/10.1073/pnas.91.26.12619.

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Devine, Jerry H., Cari Countryman, and Thomas O. Baldwin. "Nucleotide sequence of the luxR and luxI genes and structure of the primary regulatory region of the lux regulon of Vibrio fischeri ATCC 7744." Biochemistry 27, no. 2 (January 26, 1988): 837–42. http://dx.doi.org/10.1021/bi00402a052.

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Shadel, G. S., and T. O. Baldwin. "The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene." Journal of Bacteriology 173, no. 2 (1991): 568–74. http://dx.doi.org/10.1128/jb.173.2.568-574.1991.

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Koch, B., T. Liljefors, T. Persson, J. Nielsen, S. Kjelleberg, and M. Givskov. "The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors." Microbiology 151, no. 11 (November 1, 2005): 3589–602. http://dx.doi.org/10.1099/mic.0.27954-0.

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The function of LuxR homologues as quorum sensors is mediated by the binding of N-acyl-l-homoserine lactone (AHL) signal molecules to the N-terminal receptor site of the proteins. In this study, site-directed mutagenesis was carried out of the amino acid residues comprising the receptor site of LuxR from Vibrio fischeri, and the ability of the L42A, L42S, Y62F, W66F, D79N, W94D, V109D, V109T and M135A LuxR mutant proteins to activate green fluorescent protein expression from a PluxI promoter was measured. X-ray crystallographic studies of the LuxR homologue TraR indicated that residues Y53 and

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Adar, Y. Y., M. Simaan, and S. Ulitzur. "Formation of the LuxR protein in the Vibrio fischeri lux system is controlled by HtpR through the GroESL proteins." Journal of Bacteriology 174, no. 22 (1992): 7138–43. http://dx.doi.org/10.1128/jb.174.22.7138-7143.1992.

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Rui, Haopeng. "Role of Alkaline Serine Protease, Asp, in Vibrio alginolyticus Virulence and Regulation of Its Expression by LuxO-LuxR Regulatory System." Journal of Microbiology and Biotechnology 19, no. 5 (May 28, 2009): 431–38. http://dx.doi.org/10.4014/jmb.0807.404.

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