Academic literature on the topic 'Vibrio marinus'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Vibrio marinus.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Vibrio marinus"
1
Urakawa, Hidetoshi, Kumiko Kita-Tsukamoto, and Kouichi Ohwada. "Restriction fragment length polymorphism analysis of psychrophilic and psychrotrophic Vibrio and Photobacterium from the north-western Pacific Ocean and Otsuchi Bay, Japan." Canadian Journal of Microbiology 45, no. 1 (January 1, 1999): 67–76. http://dx.doi.org/10.1139/w98-128.
Full textAbstract:
Typing and identification of 60 marine psychrophilic and psychrotrophic vibrios isolated from the north-western Pacific Ocean and coastal environment of Japan were performed by restriction fragment length polymorphism analysis on the basis of polymerase chain reaction amplified 16S rDNA. We obtained 15 operational taxonomic units (OTUs) by digestion with four restriction endonucleases (HhaI, DdeI, RsaI, and Sau3AI); four large groups were obtained from the neighbor-joining method. Significant differences were observed in OTU composition between isolates from the deep sea and coastal areas. Vibrio marinus and Photobacterium species were the dominant culturable vibrios in the deep sea areas, while Vibrio splendidus like species were the dominant culturable vibrios in a coastal area of Japan.Key words: restriction analysis, Vibrio, Photobacterium, Vibrio marinus (Moritella marina), Vibrio splendidus.
2
Tall, B. D., J. F. La Peyre, J. W. Bier, M. D. Miliotis, D. E. Hanes, M. H. Kothary, D. B. Shah, and M. Faisal. "Perkinsus marinus Extracellular Protease Modulates Survival of Vibrio vulnificus in Eastern Oyster (Crassostrea virginica) Hemocytes." Applied and Environmental Microbiology 65, no. 9 (September 1, 1999): 4261–63. http://dx.doi.org/10.1128/aem.65.9.4261-4263.1999.
Full textAbstract:
ABSTRACT The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificusin oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced byP. marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues.
3
BAUMEISTER, LESLIE, MONA E. HOCHMAN, JOHN R. SCHWARZ, and ROBIN BRINKMEYER. "Occurrence of Vibrio vulnificus and Toxigenic Vibrio parahaemolyticus on Sea Catfishes from Galveston Bay, Texas." Journal of Food Protection 77, no. 10 (October 1, 2014): 1784–86. http://dx.doi.org/10.4315/0362-028x.jfp-14-175.
Full textAbstract:
Dorsal and pectoral fin spines from two species of sea catfishes (Bagre marinus and Ariopsis felis) landed at 54 sites in Galveston Bay, Texas, and its subbays from June to October 2005 were screened with traditional cultivation-based assays and quantitative PCR assays for Vibrio vulnificus and Vibrio parahaemolyticus. V. vulnificus was present on 51.2% of fish (n = 247), with an average of 403 ±337 SD cells g−1. V. parahaemolyticus was present on 94.2% (n = 247); 12.8% tested positive for the virulence-conferring tdh gene, having an average 2,039 ± 2,171 SD cells g−1. The increasing trend in seafood consumption of “trash fishes” from lower trophic levels, such as sea catfishes, warrants evaluation of their life histories for association with pathogens of concern for human consumption.
4
Langridge, Patricia, R. D. Haight, and R. Y. Morita. "Use of heat for obtaining malic dehydrogenase from cells of Vibrio marinus." Zeitschrift für allgemeine Mikrobiologie 8, no. 3 (January 24, 2007): 221–23. http://dx.doi.org/10.1002/jobm.19680080308.
Full text
5
Masuda, K. V., and L. J. Albright. "Hydrostatic pressure effects upon cellular leakage and active transport by Vibrio marinus." Zeitschrift für allgemeine Mikrobiologie 18, no. 10 (January 24, 2007): 731–40. http://dx.doi.org/10.1002/jobm.19780181005.
Full text
6
Tokárová, Viola, Ayyappasamy Sudalaiyadum Perumal, Monalisha Nayak, Henry Shum, Ondřej Kašpar, Kavya Rajendran, Mahmood Mohammadi, et al. "Patterns of bacterial motility in microfluidics-confining environments." Proceedings of the National Academy of Sciences 118, no. 17 (April 19, 2021): e2013925118. http://dx.doi.org/10.1073/pnas.2013925118.
Full textAbstract:
Understanding the motility behavior of bacteria in confining microenvironments, in which they search for available physical space and move in response to stimuli, is important for environmental, food industry, and biomedical applications. We studied the motility of five bacterial species with various sizes and flagellar architectures (Vibrio natriegens, Magnetococcus marinus, Pseudomonas putida, Vibrio fischeri, and Escherichia coli) in microfluidic environments presenting various levels of confinement and geometrical complexity, in the absence of external flow and concentration gradients. When the confinement is moderate, such as in quasi-open spaces with only one limiting wall, and in wide channels, the motility behavior of bacteria with complex flagellar architectures approximately follows the hydrodynamics-based predictions developed for simple monotrichous bacteria. Specifically, V. natriegens and V. fischeri moved parallel to the wall and P. putida and E. coli presented a stable movement parallel to the wall but with incidental wall escape events, while M. marinus exhibited frequent flipping between wall accumulator and wall escaper regimes. Conversely, in tighter confining environments, the motility is governed by the steric interactions between bacteria and the surrounding walls. In mesoscale regions, where the impacts of hydrodynamics and steric interactions overlap, these mechanisms can either push bacteria in the same directions in linear channels, leading to smooth bacterial movement, or they could be oppositional (e.g., in mesoscale-sized meandered channels), leading to chaotic movement and subsequent bacterial trapping. The study provides a methodological template for the design of microfluidic devices for single-cell genomic screening, bacterial entrapment for diagnostics, or biocomputation.
7
Birkbeck, T. H., B. Billcliffe, A. Laidler, and D. I. Cox. "The relationship between Aeromonas sp. NCIMB 2263, a causative agent of skin lesions in Atlantic salmon, Vibrio marinus (Moritella marina) and Vibrio viscosus." Journal of Fish Diseases 23, no. 4 (July 2000): 281–83. http://dx.doi.org/10.1046/j.1365-2761.2000.00228.x.
Full text
8
SOKOLOVA, INNA M., JAMES D. OLIVER, and LARRY J. LEAMY. "AN AFLP APPROACH TO IDENTIFY GENETIC MARKERS ASSOCIATED WITH RESISTANCE TO VIBRIO VULNIFICUS AND PERKINSUS MARINUS IN EASTERN OYSTERS." Journal of Shellfish Research 25, no. 1 (April 2006): 95–100. http://dx.doi.org/10.2983/0730-8000(2006)25[95:aaatig]2.0.co;2.
Full text
9
Ben Dhia Thabet, O., M. L. Fardeau, C. Suarez-Nuñez, M. Hamdi, P. Thomas, B. Ollivier, and D. Alazard. "Desulfovibrio marinus sp. nov., a moderately halophilic sulfate-reducing bacterium isolated from marine sediments in Tunisia." International Journal of Systematic and Evolutionary Microbiology 57, no. 9 (September 1, 2007): 2167–70. http://dx.doi.org/10.1099/ijs.0.64790-0.
Full textAbstract:
Two novel sulfate-reducing bacterial strains, designated E-2T and IMP-2, were isolated from geographically distinct locations. Strain E-2T was recovered from marine sediments near Sfax (Tunisia), whereas strain IMP-2 originated from oilfield production fluids in the Gulf of Mexico. Cells were Gram-negative, non-sporulated, motile, vibrio-shaped or sigmoid. They were strictly anaerobic, mesophilic and moderately halophilic. Sulfate, sulfite, thiosulfate and elemental sulfur served as electron acceptors, but not nitrate or nitrite. H2 (with acetate as carbon source), formate, fumarate, lactate, malate, pyruvate, succinate and fructose were used as electron donors in the presence of sulfate as terminal electron acceptor. Lactate was oxidized incompletely to acetate. Fumarate and pyruvate were fermented. Desulfoviridin and c-type cytochromes were present. 16S rRNA gene sequence analysis of the two strains showed that they were phylogenetically similar (99.0 % similarity) and belonged to the genus Desulfovibrio, with Desulfovibrio indonesiensis and Desulfovibrio gabonensis as their closest phylogenetic relatives. The G+C content of the DNA was respectively 60.4 and 62.7 mol% for strains E-2T and IMP-2. DNA–DNA hybridization experiments revealed that the novel strains had a high genomic relatedness, suggesting that they belong to the same species. We therefore propose that the two isolates be affiliated to a novel species of the genus Desulfovibrio, Desulfovibrio marinus sp. nov. The type strain is strain E-2T (=DSM 18311T =JCM 14040T).
10
Hernández Mendoza, Dulce Maripaz, Pablo San Martín del Ángel, Catya Jiménez Torres, and Rosa Idalia Hernández Herrera. "Monitoreo de vibrio spp. en ostiones Crassostrea virginica de las lagunas de Tamiahua y Tampamachoco, Veracruz, México." Revista Biológico Agropecuaria Tuxpan 9, no. 1 (July 1, 2021): 122–41. http://dx.doi.org/10.47808/revistabioagro.v9i1.346.
Full textAbstract:
Vibrio es un género ampliamente distribuído en ambientes estuarinos y marinos, adaptable y con rápida propagación de sus cepas. Los moluscos bivalvos son reservorios potenciales de Vibrios, su consumo está relacionado con el desarrollo de vibriosis leve como gastroenteritis hasta septicemia grave o la muerte. Por lo cual es importante el monitoreo continuo en las áreas de extraccion y distribución para evaluar el riesgo para la salud humana derivado del consumo de moluscos y garantizar la inocuidad de estos bivalvos. En el presente estudio se determinaron las diferencias estacionales en la presencia y abundancia de Vibrios durante las fases de recolección (bancos ostrícolas) y post-recolección (cooperativas y restaurantes) en ostiones Crassostrea virginica de las lagunas de Tamiahua y Tampamachoco, Veracruz, México. Las muestras fueron obtenidas de trece sitios al azar durante el año 2018. El aislamiento bacteriano se realizó por el método de siembra por dilución en medio TCBS y recuento en placa de acuerdo a la NOM-031-SSA1-1993. El análisis estadístico indicó que no existieron diferencias espaciales significativas, no obstante, se presentaron diferencias estacionales. En esta investigación, se evidencia por primera vez, la presencia de Vibrio spp. en ostiones de las cooperativas de estudio. También se reporta la ausencia de este género bacteriano en los restaurantes adyacentes. En general, los resultados muestran la necesidad de aplicar medidas dirigidas a reducir la presencia de Vibrios durante la fase de post-recolección de ostiones, con la finalidad de disminuir el riesgo de infección por Vibrio spp. en los consumidores.
Dissertations / Theses on the topic "Vibrio marinus"
1
Robino, Etienne. "Etude des amibes marines et de leurs interactions avec les vibrios pathogènes d’huître." Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTG041.
Full textAbstract:
Les amibes libres dans les environnements aquatiques utilisent la phagocytose des bactéries pour leur nutrition. Selon l’hypothèse de l’évolution fortuite de la virulence, les mécanismes cellulaires et moléculaires de la phagocytose étant conservés des amibes aux cellules immunitaires des animaux, la prédation exercée par les amibes pourraient favoriser l’émergence de bactéries pathogènes résistantes à la phagocytose. Depuis 2008, les huîtres creuses Crassostrea gigas sont victimes d’épisodes de surmortalités en France. Cette pathologie poly-microbienne implique le virus Herpes OsHV-1 µvar qui provoque une immunosuppression des huîtres qui sont alors colonisées par divers bactéries pathogènes opportunistes dont des vibrios induisant la mort de l’animal. V. tasmaniensis LGP32 est un pathogène intracellulaire facultatif des hémocytes d’huître qui résiste à la phagocytose et détruit les hémocytes en utilisant un certain nombre de facteurs de virulence. Nous avons donc entrepris d’étudier les interactions entre les amibes libres de l’environnement ostréicole et les vibrios afin de vérifier si certains mécanismes de virulence pouvaient aussi jouer un rôle dans ce type d’interactions. En réalisant des échantillonnages sur le terrain, nous avons mis en évidence que l’interaction entre vibrios et amibes est écologiquement réaliste, et observé une faible diversité de protistes hétérotrophes près des tables ostréicoles de la lagune de Thau par rapport à d’autres environnements moins anthropisés. Des études fonctionnelles entre LGP32 et l’amibe Vannella sp. AP1411 ont montré que LGP32 est capable de résister à la prédation par les amibes impliquant certains facteurs de virulence comme la métalloprotéase Vsm et la pompe d’efflux du cuivre de type P-ATPase CopA qui sont aussi impliqués dans l’interaction de LGP32 avec les huîtres. En revanche, d’autres facteurs de virulence impliqués chez l’huître ne le sont pas dans la résistance à la prédation par les amibes indiquant que certains facteurs sont impliqués dans des interactions avec divers hôtes tandis que d’autres seraient impliqués dans des interactions plus spécifiquesFree living amoebae inhabit aquatic environments and use phagocytosis of bacteria for their nutrition. According to the hypothesis of coincidental evolution of virulence, the cellular and molecular mechanisms of phagocytosis being preserved from amoebae to the immune cells of animals, the predation exerted by amoebae could favor the emergence of pathogenic bacteria resistant to phagocytosis. Since 2008, Crassostrea gigas oysters have suffered from over-mortality in France. This poly-microbial disease involves the Herpes OsHV-1 μvar virus which causes an immunosuppression of oysters that are then colonized by various opportunistic pathogenic bacteria including vibrios inducing the death of the animal. V. tasmaniensis LGP32 is a facultative intracellular pathogen of oyster hemocytes that resists phagocytosis and destroys hemocytes using different virulence factors. We have therefore undertaken to study the interactions between marine amoebae of the oyster environment and the vibrios in order to verify if some mechanisms of virulence could also play a role in this type of interactions. By performing field sampling, we demonstrated that the interaction between vibrios and amoebae is ecologically realistic and observed a low diversity of heterotrophic protists near the oyster tables of the Thau Lagoon compared to other less anthropogenic environments. Functional studies between LGP32 and the amoeba Vannella sp. AP1411 showed that LGP32 is able to resist amoeba predation involving certain virulence factors such as Vsm metalloprotease and CopA P-ATPase copper efflux pump which are also involved in the interaction of LGP32 with oysters. In contrast, other virulence factors implicated in the oyster are not involved in amoeba-predation resistance indicating that some factors are involved in interactions with various hosts while others would be involved in more specific interactions
2
Poirier, Aurore. "Etude comparative des interactions Vibrio - phagocytes dans l'environnement marin." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS062/document.
Full textAbstract:
Des souches de Vibrio appartenant au clade Splendidus sont retrouvées de manière récurrente lors des mortalités estivales d’huîtres juvéniles. La souche V. tasmaniensis LGP32 est un pathogène intracellulaire facultatif des hémocytes d’huître, dont elle altère les fonctions de défense. Nous nous intéressons ici aux interactions entre les phagocytes et V. tasmaniensis LGP32, à diverses échelles (moléculaire, cellulaire et environnementale). Dans une première partie, nous avons découvert un mécanisme antimicrobien, encore inconnu chez C. gigas : la formation de pièges d'ADN extracellulaire (ETs). Ces ETs sont associés à des histones antimicrobiennes et sont capables de piéger des bactéries. Comme chez les vertébrés, la formation de ces ETs est dépendante de la production d'espèces réactives de l'oxygène. De plus, la présence de ces ETs a été confirmée in vivo et a été associée à une accumulation d'histones antimicrobiennes dans les tissus, suite à une blessure ou à une infection. Dans une seconde partie,nous avons étudié les interactions entre V. tasmaniensis LGP32 et des protistes hétérotrophes présents dans l’environnement des huîtres, tels que les amibes et les ciliés, qui se nourrissent de micro-organismes par phagocytose. Un résultat important de cette thèse a été de montrer que V. tasmaniensis LGP32 résiste également à la phagocytose des protistes hétérotrophes environnementaux, de manière intracellulaire. C'est à notre connaissance la première fois que les mécanismes d’interaction entre des bactéries pathogènes d'origine marine et des amibes marines sont décrits. Les amibes que nous avons isolées étant présentes dans l'environnement direct des huîtres, nous pouvons donc supposer que la pression de sélection exercée par les phagocytes environnementaux pourrait favoriser l’émergence de traits de virulence et/ou de résistance à la phagocytose parmi les bactéries marines, comme c’est le cas pour V. tasmaniensis LGP32Vibrio strains belonging to the Splendidus clade have been repeatedly found in juvenile diseased oysters affected by summer mortalities. V. tasmaniensis LGP32 is an intracellular pathogen of oyster hemocytes which has been reported to alter the oxidative burst and inhibit phagosome maturation.We here focus on the interactions between phagocytes and V. tasmaniensis LGP32, at molecular, cellular and environmental scales. In the first part of this work, we uncover anunknown antimicrobial mechanism of C. gigas hemocytes: the formation of DNA extracellular traps (ETs). These ETs are associated with antimicrobial histones and are able to entrap bacteria. As in vertebrates, ETs formation depends on reactive oxygen species production. In addition, the presence of ETs was confirmed in vivo and has been associated with antimicrobial histones accumulation in tissues, in response to injury or infection. In the second part of this work, we studied the interactions between V. tasmaniensis LGP32 and heterotrophic protists found in the oyster’s environment, such as amoebae and ciliates, which feed on microorganisms by phagocytosis. An important result of this workwas that V. tasmaniensis LGP32 resists to phagocytosis by environmental heterotrophic protists, as well as to oyster hemocytes. To our knowledge, this is the first mechanical description of an interaction between marine amoebae and marine pathogenic bacteria. As the amoebae were isolated from the direct environment of oysters, we can presume that the selective pressure exerted by environmental phagocytes could select for virulence and/or phagocytosis resistance traits in marine bacteria as in the case of V. tasmaniensis LGP32
3
Dueñas, Peña Talia Greta Amalia. "Recuento de Vibrio parahaemolyticus Kanagawa positivo en especies marinas de consumo en Lima Metropolitana y Callao." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2008. https://hdl.handle.net/20.500.12672/1628.
Full textAbstract:
El objetivo del presente trabajo fue hacer un recuento de Vibrio parahaemolyticus Kanagawa positivo a partir de 50 muestras de pescados, moluscos y crustáceos crudos procedentes de terminales pesqueros, muelles y supermercados de consumo en Lima Metropolitana y Callao entre noviembre de 1999 y abril de 2000. El análisis microbiológico se realizó de acuerdo a la metodología recomendada por el Manual Bacteriológico Analítico (BAM, 7 ed.). Se halló Vibrio parahaemolyticus Kanagawa positivo en 4 muestras aislándose 5 cepas con las características bioquímicas correspondientes de un total de 568 cepas sospechosas. Los valores hallados de Número Más Probable (NMP) fueron en pescados y crustáceos de 3/g cada uno y en moluscos un valor mínimo de 3/g y un máximo de 7,4/g. Las muestras que presentaron Vibrio parahaemolyticus Kanagawa positivo (n=4) representaron un 8% del total de especies marinas (n=50), siendo los porcentajes hallados en pescados 2% (n=1), moluscos 4% (n=2) y crustáceos 2% (n=1).
-- Palabras Clave: Vibrio parahaemolyticus, pescados, crustáceos, moluscos, Kanagawa positivo, NMP, Lima Metropolitana y Callao.-- The aim of this research was performing a count of Vibrio parahaemolyticus Kanagawa positive out of 50 samples including raw fish, mollusks and crustaceans collected from fishermen’s wharf, fisheries, and supermarkets of edible character in Metropolitan Lima and Callao between november 1999 and april 2000. The microbiological analysis was performed according to Bacteriological Analytical Manual (BAM, 7 ed.). Vibrio parahaemolyticus Kanagawa positive was found in 4 samples from 5 strains with biochemical features that met those of Vibrio parahaemolyticus out of a total of 568 analized strains. The Most Probable Number (NMP) values found are as follows: Fish and crustaceans 3/g each, while molluscs had a minimum value of 3/g and a maximum of 7,4/g. Vibrio parahaemolyticus Kanagawa positive samples (n=4) represented 8% out of the total marine samples (n=50), being the percentages found in fish 2% (n=1), mollusks 4% (n=2) and crustaceans 2% (n=1) out of the total number of samples as well. -- Key Words: Vibrio parahaemolyticus, fish, mollusks, crustaceans, Kanagawa positive, MPN, Metropolitan Lima and Callao.
Tesis
4
Bienlien, Lydia M. "Influence of Perkinsus Marinus Infection and Oyster Health on Levels of Human-Pathogenic Vibrios in Oysters." W&M ScholarWorks, 2016. https://scholarworks.wm.edu/etd/1477068161.
Full textAbstract:
The eastern oyster Crassostrea virginica is an ecologically and commercially important species whose natural populations have been devastated by overharvesting, habitat destruction, and disease, but the rapid growth of oyster aquaculture has shown potential to restore the economic significance of this species. A key threat to the growth and sustainability of oyster aquaculture is the association of human-pathogenic Vibrio bacteria with product marketed for raw consumption. Two Vibrio species, Vibrio vulnificus and Vibrio parahaemolyticus, are the causes of the highest rates of seafood consumption-related mortality and gastrointestinal illness, respectively. Identification of the factors influencing V. vulnificus and V. parahaemolyticus prevalence and intensity in oysters is fundamental to better risk management. Within the oyster, these bacterial species interact with the same tissues as the prevalent oyster parasite, Perkinsus marinus, yet little is known about the effect of P. marinus infection on bacterial levels. Answering the fundamental question of whether P. marinus correlates with V. vulnificus and V. parahaemolyticus levels in oysters was the focus of this research. Oysters were deployed in the York River, Gloucester Point, VA, where both Vibrio species and P. marinus are endemic, and were sampled at five time points when levels of both P. marinus and Vibrio spp. were expected to be high in oysters. Abundance of all three organisms and pathogenic strains of V. parahaemolyticus were determined in individual oysters using molecular methods to investigate potential correlations between parasite and bacterial abundance. Additionally, the levels of V. vulnificus and V. parahaemolyticus in relation to histopathology associated with P. marinus infection and other conditions were determined. The following year, manipulation of P. marinus disease progression, which is slowed by lower salinities and favored by higher salinities, was attempted by deploying oysters at two additional sites of different salinities to gain insight into whether the timing of P. marinus infection emergence directly influences Vibrio levels. No correlation was observed between total abundance of P. marinus and either V. vulnificus or V. parahaemolyticus. Manipulation of P. marinus disease progression produced no effect on P. marinus emergence, so this yielded no insight into P. marinus-Vibrio interactions. Histopathological analyses did not reveal any correlations between P. marinus ranking, distribution, or associated tissue damage and Vibrio spp. levels. Though few in number, oysters infected by Haplosporidium nelsoni were characterized by higher levels of V. vulnificus, and oysters of peak gametogenic development had significantly higher levels of pathogenic strains of V. parahaemolyticus. The results with regard to H. nelsoni and gametogenic state warrant further study. The primary conclusion of this study is that oyster health has little influence on levels of human-pathogenic Vibrio species in oysters, inter-host variability in Vibrio levels is likely explained by other factors.
5
CARVALHO, Joana Angélica Lyra Vogeley de. "Efeito do uso de bactérias probióticas na sobrevivência de larvas de Litopenaeus vannamei expostas à infecção experimental por Vibrio spp." Universidade Federal Rural de Pernambuco, 2011. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6339.
Full textAbstract:
Submitted by (edna.saturno@ufrpe.br) on 2017-02-14T15:03:25Z
No. of bitstreams: 1
Joana angelica L V Carvalho.pdf: 451922 bytes, checksum: 66f7ebcb01d8574ab7c4cced0c81dce6 (MD5)Made available in DSpace on 2017-02-14T15:03:25Z (GMT). No. of bitstreams: 1 Joana angelica L V Carvalho.pdf: 451922 bytes, checksum: 66f7ebcb01d8574ab7c4cced0c81dce6 (MD5) Previous issue date: 2011-02-10
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
This study aimed to evaluate the survival of Litopenaeus vannamei shrimp larvae treated with probiotic bacteria and subsequently infected by Vibrio harveyi and Vibrio alginolyticus. Shrimp were kept in two 80L tanks under similar culture conditions, but only one tank was previously treated with commercial probiotic (Bacillus spp.) added daily in the water at a concentration of 105 CFU/ml. This protocol was maintained until the animals reach the stage of development needed for the infection experiments by Vibrio spp. Three independent infection experiments were performed: Zoea1 to Zoea3, Mysis1 to Mysis3 and Postlarvae (PL)10 to PL14 with the following treatments with four replicates each: only V. harveyi; commercial probiotic + V. harveyi; only V. alginolyticus; commercial probiotic + V. alginolyticus; and without Vibrio spp. and probiotic (Control). In the experiment with PL, only the treatments with V. harveyi; commercial probiotic + V. harveyi and control were used. Vibrio species were inoculated only at the beginning of each experiment at a concentration of 107 CFU/ml. At the end of experiments, a sample of water and shrimp from each experimental unit was submitted to quantification of Vibrio spp. Additionally, was realized an antagonism test of Bacillus spp. against Vibrio spp. In the experiments with zoea and mysis, larvae cultured in treatment V. harveyi had a significantly lower survival when compared with treatments V. alginolyticus and control. There was a significant increase in survival of zoea and mysis larvae treated with probiotic + V. harveyi with 81.07 and 90.13%, differentiating the larvae infected only with V. harveyi with 12.80 and 69.13%, respectively. There were no significant differences in survival of postlarvae among treatments. Total Vibrio spp. counts decreased in the water and shrimp of treatments with the use of probiotic. The probiotic (Bacillus spp.) showed inhibitory activity against Vibrio spp. in vitro. The results indicated an increase in the survival of early larval stages (zoea and mysis) infected by V. harveyi after treatment with Bacillus spp. The administration of probiotic bacteria is a promising alternative for the prevention of Vibrio spp. in larviculture of L. vannamei.
O presente estudo teve como objetivo avaliar a sobrevivência de larvas de Litopenaeus vannamei tratadas com bactérias probióticas e posteriormente infectadas por Vibrio harveyi e Vibrio alginolyticus. Os camarões foram mantidos em dois tanques de 80L nas mesmas condições, mas apenas um deles foi previamente tratado com probiótico comercial (Bacillus spp.) adicionado diariamente na água na concentração de 105 UFC/ml. Este protocolo foi mantido até os animais alcançarem o estágio de desenvolvimento larval necessário para os experimentos de infecção por Vibrio spp. Foram realizados três experimentos independentes de infecção: Zoea1 a Zoea3, Mísis1 a Mísis3 e Pós-larvas (PL)10 a PL14 com os seguintes tratamentos com quatro repetições cada: somente V.harveyi; probiótico comercial + V. harveyi; somente V. alginolyticus; probiótico comercial + V. alginolyticus; sem Vibrio spp. e probiótico (controle). No experimento com PL, somente os tratamentos com V. harveyi; probiótico comercial + V. harveyi e controle foram utilizados. As espécies de Vibrio foram inoculadas apenas no início de cada experimento na concentração de 107 UFC/ml. Ao final dos experimentos, uma amostra de água e camarão de cada parcela experimental foi submetida à quantificação de Vibrio spp. Adicionalmente foi realizado um teste de antagonismo in vitro de Bacillus spp. contra Vibrio spp. Nos experimentos com zoea e mísis, as larvas cultivadas no tratamento V. harveyi apresentaram uma sobrevivência significativamente inferior quando comparadas com as dos tratamentos V. alginolyticus e controle. Houve um aumento significativo na sobrevivência de larvas zoea e mísis tratadas com probiótico + V. harveyi com 81,07 e 90,13%, em comparação com as larvas infectadas apenas com V. harveyi com 12,80 e 69,13%, respectivamente. Não foram observadas diferenças significativas na sobrevivência das pós-larvas entre tratamentos. A quantidade total de Vibrio spp. na água e camarões diminuiu nos tratamentos com o uso de probiótico. O probiótico (Bacillus spp.) apresentou atividade inibitória contra Vibrio spp. in vitro. Os resultados indicaram um aumento na sobrevivência dos estágios larvais iniciais (zoea e mísis) infectadas por V. harveyi após tratamento com Bacillus spp. A administração de bactérias probióticas é uma alternativa promissora para a prevenção de Vibrio spp. na larvicultura de L. vannamei.
6
Trubitsyn, Denis. "Magnetosome formation in marine vibrio MV-1." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/7589.
Full textAbstract:
Marine vibrio MV-1 is a magnetotactic bacterium capable of aligning its cell in response to the Earth’s magnetic field. This ability is due to the presence of chainlike structures comprising magnetosomes, magnetite particles enclosed in a lipid membrane with associated proteins. Strain MV-1 differs from other, bettercharacterized strains of magnetotactic bacteria as the cells produce higher amounts of biomagnetite per litre of culture and its magnetosomes are unique in shape. This study investigates the presence and organisation of a gene cluster termed a “magnetosome island” within the genome of MV-1. In other magnetotactic bacteria this genomic region has been shown to contain many of the genes associated with magnetosome formation but has not been previously investigated for MV-1. One of the conserved fragments of this region was amplified using degenerate primers followed by extension of the known sequence using inverse PCR based technique and constructing plasmid libraries. Sequencing of the genome of strain MV-1 was accomplished as a part of this study. Significant work was done on comparison of the sequence quality obtained from SOLEXA, 454 and Sanger sequencing technologies. A number of obtained contigs were joined manually and the resulting sequence was automatically annotated using RAST. The obtained genome sequence of 3.6 Mb with a G+C content of 54.3 % was preliminarily analysed and used to search for magnetosome related genes. This study also analysed proteins associated with the magnetosomes of strain MV-1 using MALDI-TOF, LC-MS and Orbitrap mass spectrometry. These approaches allowed the identification of a number of proteins in the isolated magnetosome membrane fraction. Some of these proteins have very low similarity with other characterized proteins (either in magnetotactic bacteria or in other organisms). Another significant point is that genes that code for proteins such as MamR, MamK and MmsF were found to be present in several homologous copies within the “magnetosome island” of MV-1. Interestingly, this study shows that all homologous copies of these proteins were identified in the magnetosome membrane fraction. Generation of knock-out mutants of several specific genes from the “magnetosome island” of strain MV-1 was attempted; constructs were made based on suicide plasmids carrying the cre-lox or I-SceI systems. Despite altering numerous experimental conditions it was not possible to obtain conclusive evidence of the isolation of MV-1 transconjugants containing the integrated constructs. In order to investigate the cell localization of the magnetosome associated protein CAV30779.1, an enhanced green fluorescent protein (EGFP) fusion based construct was generated and transferred into MV-1 cells. The EGFP fluorescent protein fusions within the cells were detected by microscopy. This study reveals novel information about magnetosome formation in marine vibrio MV-1. The obtained results provide an important foundation for further investigation of this organism and contribute towards broadening the knowledge of the complex process of magnetosome formation in bacteria.
7
Flores, Dominick Violeta de Jesús. "Aislamiento y caracterización de un bacteriófago con actividad lítica para Vibrio fluvialis." Master's thesis, Universidad Nacional Mayor de San Marcos, 2017. https://hdl.handle.net/20.500.12672/7007.
Full textAbstract:
Aisla un bacteriófago lítico denominado Vf1, procedente del mar peruano, capaz de infectar a Vibrio fluvialis, bacteria que afecta humanos y organismos acuáticos. El bacteriófago Vf1 fue caracterizado con respecto a su morfología, estabilidad térmica, diferentes rangos de pH, sensibilidad frente al cloroformo, exposición a la luz UV, rango de hospederos y la curva de crecimiento de un paso. La microscopía electrónica de transmisión, evidenció cabeza de forma icosaedrica con un diámetro de 82.773 nm y un talo largo no contráctil de 142.318 nm de longitud, características propias a la familia Siphoviridae. El fago Vf1, fue resistente al calor a 20°C por 30 minutos, perdiendo su viabilidad a partir de 50 y 60°C; y en valores de pH entre 7 y 8 mantuvo títulos elevados, disminuyendo el título entre 5 y 6, siendo nulo a valores de pH ácido. La viabilidad frente a la exposición al cloroformo, evidenció una reducción en el título del 50% y frente a la luz UV, la reducción fue del 100% a 90 segundos de exposición. En la curva de crecimiento de un paso evidenció un período de latencia de 20 minutos y el tamaño de la explosión o burst size fue 602 partículas por centro infectivo. Los parámetros biológicos evaluados en este estudio evidenciaron el potencial del bacteriófago Vf1 para poder ser utilizado en fagoterapia, además, la metodología evaluada podría ser utilizado para el aislamiento de otros bacteriófagos de ambientes marinos.Tesis
8
Girard, Léa. "Quorum Sensing in Vibrio spp. : AHL diversity, temporal dynamic and niche partitioning." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066650/document.
Full textAbstract:
Chez les Vibrio spp., le QS est impliqué dans de nombreuses fonctions comme la colonisation de niche écologiques, les stratégies de survie ou encore la virulence. Cependant, pour la majorité des espèces de Vibrio, la diversité des AHLs produites reste largement sous-estimée et l'étude du QS est encore limitée à quelques espèces modèles ou pathogènes. Toutefois, dans les environnements aquatiques, ces espèces sont minoritaires et les espèces les plus abondantes ne sont que très peu étudiées. Nos résultats ont révélé une importante diversité d'AHLs mais aussi, de façon surprenante, une hétérogénéité dans les phénotypes de production d'AHL au sein d'une même espèce de Vibrio. Pour la première fois, nous avons mis en évidence qu'une même souche de Vibrio pouvait présenter des phénotypes de production d'AHLs différent au cours du temps et une approche statistique a révélé l'implication de certains déterminants biotiques et abiotiques dans ces variations temporelles. Par ailleurs, une approche à micro-échelle a révélé une structuration des populations de Vibrio en unités fonctionnelles constituées de souches phylogénétiquement proches qui partagent des niches écologiques spécifiques et des comportements sociaux. Nos résultats ont mis en évidence que les modalités de communication pouvaient être hétérogènes suggérant l'absence d'un langage commun au sein de ces unités fonctionnelles. En conclusion, ce travail de thèse a permis d'apporter de nouvelles connaissances sur le QS chez les Vibrio dans l'environnement marin, de la souche à la population, et propose une vision intégrée des mécanismes de régulation de la production d'AHLs dans l'environnementQuorum sensing is an important mechanism among Vibrio species and is involved in many vital functions such as niche colonization, survival strategies or virulence. However, AHL diversity still largely underestimated for the majority of Vibrio species and the current knowledge on AHL-mediated QS is limited to a few pathogenic or bioluminescent species. Nonetheless, these species are weakly abundant in seawater while dominant species in the environment are poorly studied. Our results revealed a unexpected diversity of AHL molecules but also a quite surprising intra-species diversity of AHL production phenotypes. For the first time, we showed that different isolates of a single genotype switched between different AHL production phenotypes among time and we revealed the potential involvement of abiotic and biotic parameters in these variations. However, it appears that when studied at a microscale, Vibrio populations are showing a functional structuration in ecological units consisting of phylogenetically close strains sharing habitat and social traits. In this context, it was necessary to determine if these different AHL production phenotypes were associated to different micro-habitats in the water column. We did not demonstrate that a common language was spoken within ecological populations. This thesis work provide new insights on AHL-mediated QS among a broader range of species and among Vibrio populations and depicts the potential impact of multiple aspects of marine environments on AHL production
9
Lavezzo, Lígia Carolina. "Caracterização das especies de vibrios isoladas em amostras de água do mar, plâncton e bivalves da zona litorânea do Estado de São Paulo." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-18112015-192329/.
Full textAbstract:
Neste estudo, objetivou-se caracterizar ao nível molecular os vibrios isolados de amostras de água do mar, plâncton e bivalves do Canal de São Sebastião (n=78), Baixada Santista (n=37) e Ubatuba (n=17), analisar a susceptibilidade aos antibióticos e os principais genes associados à virulência. Observou-se sensibilidade à ciprofloxaxina, meropenem e ácido nalidíxico, resistência à ampicilina e à cefalotina, e alta porcentagem de múltipla resistência (Ubatuba: 64,7%; Baixada Santista:48,6%; Canal de São Sebastião: 43%) aos antimicrobianos. Quatro isolados foram positivos para o gene de virulência stn/sto. Por MLSA, foi possível identificar V.alginolyticus, V.fluvialis, V.campbellii e V.harveyi em Ubatuba; V.fluvialis, V.alginolyticus, V.campbellii, V.rotiferianus, V.harveyi, V.diabolicus, V.atypicus, V.coralliilyticus, V.maritimus, V.parahaemolyticus e V.tubiashii no Canal de São Sebastião; e, V.alginolyticus, V.parahaemolyticus, V.rotiferianus, V.campbellii, V.harveyi, V.communis, V.maritimus, V.fluvialis, V.fortis, V.natriegens e V.navarrensis na Baixada Santista.The aim of this study was to characterize at the molecular level Vibrio species isolated from seawater, plankton, bivalves samples from Canal de São Sebastião (n=78), Baixada Santista (n=37) and Ubatuba (n=17), to analyze antimicrobial susceptibility and the major virulence-associated genes. The results showed ciprofloxacin, meropenem, nalidixic acid sensitivity, ampicillin, and cephalothin resistance, and a significant percentage of multidrug resistance (Ubatuba: 64.7%; Baixada Santista: 48.6%; Canal de São Sebastião: 43%). Four seawater isolates were found positive for the stn/sto virulence gene. MLSA allowed the identification of V.alginolyticus, V.fluvialis, V.campbellii, V.harveyi in Ubatuba; V.fluvialis, V.alginolyticus, V.campbellii, V.rotiferianus, V.harveyi, V.diabolicus, V.atypicus, V.coralliilyticus, V.maritimus, V.parahaemolyticus and V.tubiashii in Canal de São Sebastião, and V.alginolyticus, V.parahaemolyticus, V.rotiferianus, V.campbellii, V.harveyi, V.communis, V.maritimus, V.fluvialis, V.fortis, V.natriegens, and V.navarrensis in Baixada Santista.
10
Neto, Antonio Alves dos Santos. "Biologia computacional aplicada à análise de dados de microarranjos do genoma da bactéria marinha vibrio parahaemolyticus em presença de n-acetilglicosamina." Laboratório Nacional de Computação Científica, 2010. http://www.lncc.br/tdmc/tde_busca/arquivo.php?codArquivo=210.
Full textAbstract:
A análise da expressão gênica em larga escala é de fundamental importância para a melhor compreensão do funcionamento celular e dos mecanismos de regulação gênica. Ela possibilita a medida dos níveis de expressão de milhares de genes simultaneamente, o que torna possível uma visão mais abrangente do sistema biológico. Dentre as principais técnicas experimentais disponíveis para esta finalidade, a tecnologia de microarranjo tem sido amplamente utilizada. O objetivo desta dissertação foi determinar os genes de V. parahaemolyticus que têm sua expressão induzida ou reprimida na presença do aminoaçúcar N-acetilglucosamina (NAG). V. parahaemolyticus é uma bactéria marinha, comumente encontrada na água e em associação com organismos marinhos. O NAG é um dos aminoaçúcares mais abundantes no meio marinho. Para isso, Vibro parahaemolyticus RIMD2210633, foi cultivada em dois meios como fontes de energia. O primeiro meio composto por maltose e NAG e o segundo apenas por NAG. O cultivo bacteriano foi feito em condições aeróbicas, sob baixa agitação, a 28C. Foram retiradas duas amostras do cultivo no tempo de 24 horas após o início do experimento a fim de realizar a extração de mRNA e a preparação do cDNA. Os experimentos foram feitos em três replicas. As misturas de cDNA preparadas a partir do RNA extraído de cada réplica foram utilizadas em hibridizações em lâminas de microarranjo contendo um total de 4832 ORFS do genoma de V. parahaemolyticus RIMD2210633. A análise comparativa da expressão gênica de V. parahaemolyticus nas duas condições de cultivo resultou na detecção de 59 genes com expressão induzida, 38 genes reprimidos, e 4245 sem expressão modificada (aumentada ou diminuída) na presença de NAG. No total, 523 genes foram excluídos da comparação pois a hibridização não foi satisfatória. Ocorreu uma ordenação dos genes seguindo a classificação funcional do banco de dados TIGR-CMR e KEGG. Os genes com expressão induzida, pertencem principalmente às classes de funções regulatórias, metabolismo de energia, e proteínas de transporte. Foram também encontradas proteínas PilA e de quimiotaxia, sugerindo um papel da NAG na transformação. Já os genes de expressão reprimida compreendem principalmente as funções de metabolismo de energia, endereçamento celular, e proteínas hipotéticas. O presente estudo demonstrou que NAG interfere na regulação de diferentes processos celulales, incluindo a capacidade de captura de DNA do meio por V. parahaemolyticus.Large scale gene expression analysis has fundamental importance for understanding cellular function and gene regulation mechanics. It enables the measurement of expression levels of thousands of genes simultaneously, and makes possible a wider understanding of the biological system. Among the main experimental techniques available for this purpose, microarray technology has been widely used. The objective of this work was to determine the genes of Vibrio parahaemolyticus which have their expression induced or repressed in presence of amino-sugars N-acetylglucosamine (NAG). V. parahaemolyticus is a marine bacterium, commonly found in water and in association with marine organisms. NAG is one of the most abundant amino sugars in the marine environment. For this, V. parahaemolyticus RIMD2210633, was cultivated in two media as sources of energy. The first medium consists of maltose and NAG (control) and the second only by NAG (treatment). Bacterial culture was done under aerobic conditions and low agitation at 28C. Two samples were drawn from the medium 24 hours after the experiment beginning in order to perform the extraction of mRNA and preparation of cDNA. Three replicas of the experiments were made. Mixtures of cDNA prepared from RNA extracted from each replicate were used in hybridizations in microarray slides containing a total of 4832 ORFS from the V. parahaemolyticus RIMD2210633 genome. Comparative analysis of gene expression of V. parahaemolyticus in two culture conditions resulted in detection of 59 genes with expression induced, 38 repressed genes, and 4245 without modified expression (increased or decreased) in presence of NAG. In total, 523 genes were excluded from this comparison because the hybridization was unsatisfactory. There was a gene ordination following the functional classification of the database TIGR-CMR and KEGG. The genes with induced expression mainly belong to classes of regulatory functions, energy metabolism, and transport proteins. PilA and Chemotaxis proteins were found, suggesting a role of NAG in the transformation. Repressed expression genes are mainly included in the functions of energy metabolism, cell address, and hypothetical proteins. This study demonstrated that NAG interfere in regulation of different cell processes, including the ability to capture DNA from the medium by V. parahaemolyticus.
Books on the topic "Vibrio marinus"
1
Göteborgs universitet. Dept. of General and Marine Microbiology., ed. On starvation and chemotactic responses by a marine Vibrio sp. S14. Göteborg, Sweden: Dept. of General and Marine Microbiology, University of Göteborg, 1990.
Find full text
2
Cuttle, Lisa. Dermatologic Manifestations of Infectious Disease. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199976805.003.0044.
Full textAbstract:
Toxic infectious exfoliative conditions include staphylococcal toxic shock syndrome (TSS), streptococcal toxic shock syndrome (STSS), and staphylococcal scalded skin syndrome (SSSS). All three are mediated by bacterial toxin production and are considerations in the differential diagnosis of a febrile, hypotensive patient with a rash. Meningococcemia is potentially fatal and extremely contagious with a short incubation period. Disseminated gonococcal infection (DGI) presents with tenosynovitis, dermatitis, and polyarthralgias without purulent arthritis or with purulent arthritis but without skin lesions. Ecthyma gangrenosum (EG) is a cutaneous manifestation of Pseudomonas aeruginosa infection. Rocky Mountain Spotted Fever (RMSF) is caused by Rickettsia rickettsii, most commonly transmitted by the American dog tick. Patients present with nonspecific symptoms, such as fever, headache, myalgias, arthralgias, nausea, vomiting, and abdominal pain. Finally, vibrio vulnificus is a gram-negative bacterium that causes serious wound infections, sepsis, and diarrhea in patients exposed to shellfish or marine water.
3
Pérez Reytor,, Diliana Celeste. Identificación de nuevos marcadores de virulencia en cepas no toxigénicas de vibrio parahaemolyticus. Universidad Autónoma de Chile, 2019. http://dx.doi.org/10.32457/20.500.12728/87462019dcbm7.
Full textAbstract:
Vibrio parahaemolyticus es la principal causa de gastroenteritis transmitida por mariscos en todo el mundo. La virulencia de V. parahaemolyticus se ha atribuido hasta ahora principalmente a la hemolisina directa termoestable (TDH) y la hemolisina relacionada con TDH (TRH). Recientemente el Sistema de Secreción de tipo III del cromosoma II (T3SS2), el cual codifica para varios efectores, ha sido relacionado con citotoxicidad y enterotoxicidad. Después de la aparición y posterior caída de la cepa pandémica, se han notificado casos de diarrea producidos por cepas clínicas que carecen de los genes tdh, trh y T3SS2 en muchos países, incluido Chile. Estas cepas, llamadas “no toxigénicas”, constituyen el 9-10% de los casos de diarrea a nivel mundial y aunque se han hecho avances en la descripción de los factores de virulencia de V. parahaemolyticus, la capacidad de las cepas no toxigénicas para causar enfermedad no ha sido completamente entendida. El hecho de que los genes tdh y trh se utilizan para estimar la carga de cepas patógenas en los mariscos durante el análisis de riesgo llama la atención sobre cuán fiables son estos análisis para detectar la gran variedad de cepas potencialmente patógenas presentes en las aguas y productos marinos. Por otra parte se conoce que en Vibrio, la evolución de la virulencia, parece estar estrechamente asociada a su capacidad para generar diversidad genética, en parte, a través de la modificación de la expresión génica, aunque mayoritariamente a través de transferencia genética horizontal (HGT). Con base en lo descrito anteriormente, esta propuesta hipotetiza que las cepas no toxigénicas de Vibrio parahaemolyticus han adquirido nuevos factores de virulencia mediante transferencia genética horizontal. Es por ello que el objetivo de esta tesis es: Identificar y caracterizar nuevos factores de virulencia en cepas chilenas no toxigénicas de Vibrio parahaemolyticus adquiridos mediante transferencia génica horizontal. Esta tesis está organizada en tres capítulos, el capítulo 1 comprende el marco teórico, el planteamiento del problema, la hipótesis y los objetivos. El capítulo 2, correspondiente al desarrollo del objetivo 1, en el cual se caracteriza el genoma de seis cepas no toxigénicas de V. parahaemolyticus aisladas del Sur de Chile. Uno de los principales hallazgos de este estudio fue la variabilidad genética de estas cepas al analizar su genoma accesorio. Este análisis mostró además la presencia de nuevas islas genómicas y elementos tipo profagos que codifican toxinas como zonula occludens (Zot) y repeats-in-toxin (RTX), ambas descritas en otros patógenos como V. cholerae donde se consideran factores de virulencia, aunque últimamente se ha descrito que la pérdida de RTX no afecta la virulencia de esta bacteria. En el capítulo 3 y final de esta tesis, se aborda el objetivo 2 que corresponde a la caracterización de posibles nuevos factores de virulencia, en este caso, la toxina Zonula Occludens (Zot). Aunque se sabe que Zot aumenta la permeabilidad epitelial intestinal por interacción con el receptor celular de zonulina PAR2 y esta unión desencadena una cascada de eventos intracelulares que conducen al desensamblaje de las uniones estrechas intercelulares, lo que se ha asociado con la producción de la diarrea en V. cholerae, el potencial patógeno de Zot de V. parahaemolyticus no se ha investigado aún. La cepa clínica PMC53.7, tdh/trh/T3SS2/negativa, resultó ser altamente citotóxica en cultivo celular de Caco-2 y contiene en su genoma accesorio un gen homólogo de zot. Con este antecedente, se caracterizó la toxina Zot en la cepa clínica PMC53.7 de V. parahaemolyticus y sus efectos sobre la barrera epitelial intestinal. El gen zot de PMC53.7 se clonó y se expresó en Escherichia coli BL21(DE3) y los efectos sobre la barrera epitelial intestinal se examinaron usando el modelo celular Caco-2. Se evaluó el cambio en la distribución de las proteínas de transmembrana asociadas a uniones estrechas (ZO-1 y ocludina), y en la distribución de actina en monocapas de Caco-2. Tras el tratamiento con Zot, se observó una modificación de la morfología celular. El cambio en las distribuciones de ocludina y F-actina se observó como una fragmentación de los límites brillantes de las células, con áreas de baja y alta intensidad, lo que indica una pérdida y redistribución de las proteínas asociadas a uniones estrechas. Los resultados de este trabajo sugieren que V. parahaemolyticus Zot puede contribuir a la virulencia de cepas no toxigénicas. En resumen, estos estudios han arrojado información sobre la diversidad de cepas de V. parahaemolyticus del sur del Pacífico, en especial aquellas que no poseen los principales factores de virulencia descritos para este microorganismo. Además, se caracteriza por primera vez una toxina Zot de V. parahaemolyticus en una cepa aislada de un paciente. Finalmente, los ensayos preliminares realizados en cultivo celular demostraron un posible potencial patógeno de esta toxina en la barrera epitelial intestinal.
Book chapters on the topic "Vibrio marinus"
1
McFall-Ngai, M., C. Brennan, V. Weis, and L. Lamarcq. "Mannose Adhesin—Glycan Interactions in the Euprymna Scolopes—Vibrio Fischeri Symbiosis." In New Developments in Marine Biotechnology, 273–76. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4757-5983-9_58.
Full text
2
Lizárraga-Partida, Marcial Leonardo, Irma Wong-Chang, Guadalupe Barrera-Escorcia, Alfonso, and V. Botello. "Detection of Culturable and Non-Culturable Vibrio Cholerae 01 in Mexico." In New Developments in Marine Biotechnology, 307–10. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4757-5983-9_65.
Full text
3
Andersen, Kristian Gjerrestad, Gbanaibolou Jombo, Sikiru Oluwarotimi Ismail, Yong Kang Chen, Hom Nath Dhakal, and Yu Zhang. "Damage Characterisation in Composite Laminates Using Vibro-Acoustic Technique." In Springer Proceedings in Energy, 275–82. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-63916-7_34.
Full textAbstract:
AbstractThe need to characterise in-service damage in composite structures is increasingly becoming important as composites find higher utilisation in wind turbines, aerospace, automotive, marine, among others. This paper investigates the feasibility of simplifying the conventional acousto-ultrasonic technique set-up for quick and economic one-sided in-service inspection of composite structures. Acousto-ultrasonic technique refers to the approach of using ultrasonic transducer for local excitation while sensing the material response with an acoustic emission sensor. However, this involves transducers with several auxiliaries. The approach proposed herewith, referred to as vibro-acoustic testing, involves a low level of vibration impact excitation and acoustic emission sensing for damage characterisation. To test the robustness of this approach, first, a quasi-static test was carried out to impute low-velocity impact damage on three groups of test samples with different ply stacking sequences. Next, the vibro-acoustic testing was performed on all test samples with the acoustic emission response for the samples acquired. Using the acoustic emission test sample response for all groups, the stress wave factor was determined using the peak voltage stress wave factor method. The stress wave factor results showed an inverse correlation between the level of impact damage and stress wave factor across all the test sample groups. This corresponds with what has been reported in literature for acousto-ultrasonic technique; thus demonstrating the robustness of the proposed vibro-acoustic set-up. Structural health monitoring, impact damage, acousto-ultrasonic testing, non-destructive testing.
4
Jiang, Sunny C. "Vibrio cholerae in recreational beach waters and tributaries of Southern California." In The Ecology and Etiology of Newly Emerging Marine Diseases, 157–64. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-017-3284-0_14.
Full text
5
Nirajkumar, Sojitra, Satya P. Singh, and John J. Georrge. "In Silico Identification of Drug Targets and Drug-Like Molecules against Vibrio splendidus LGP32." In Marine Niche: Applications in Pharmaceutical Sciences, 401–14. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5017-1_22.
Full text
6
Visick, Karen L., and Edward G. Ruby. "Temporal Control of Lux Gene Expression in the Symbiosis between Vibrio Fischeri and Its Squid Host." In New Developments in Marine Biotechnology, 277–79. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4757-5983-9_59.
Full text
7
Singer, John T., Jacqueline H. Edgar, and Bruce L. Nicholson. "Expression of Capsid Proteins from Infectious Pancreatic Necrosis Virus (IPNV) in the Marine Bacterium Vibrio Anguillarum." In New Developments in Marine Biotechnology, 303–6. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4757-5983-9_64.
Full text
8
Lipp, Erin K., Cesar Rodriguez-Palacios, and Joan B. Rose. "Occurrence and distribution of the human pathogen Vibrio vulnificus in a subtropical Gulf of Mexico estuary." In The Ecology and Etiology of Newly Emerging Marine Diseases, 165–73. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-017-3284-0_15.
Full text
9
Huang, Lixing, Qiancheng Gao, Youyu Zhang, Wei Xu, and Qingpi Yan. "Community Change and Pathogenicity of Vibrio." In Vibrios [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96515.
Full textAbstract:
Vibrio is a rod-shaped Gram-negative bacteria, which is widely distributed in marine and estuarine environments worldwide. It is an important component of the aquatic ecosystem and plays an important role in biogeochemical cycle. Its population dynamics are usually affected by climate and seasonal factors. Most of the Vibrios in the environment are not pathogenic, but some of them are pathogenic bacteria for human and animal, such as Vibrio cholerae, Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio anguillarum, etc., which are generally reported to be related to aquatic animal diseases and human food-borne diseases. Over the last couple of years, due to the influence of the rising seawater temperature and climate change, the incidence of diseases caused by Vibrio infection has increased significantly, which poses a great threat to human health and aquaculture. The research on pathogenic Vibrio has attracted more and more attention. The abundance and community changes of Vibrio in the environment are usually controlled by many biological and abiotic factors. The Vibrio pathogenicity is related to the virulence factors encoded by virulence genes. The process of Vibrio infecting the host and causing host disease is determined by multiple virulence factors acting together, instead of being determined by a single virulence factor. In this chapter, community changes of Vibrio, as well as the virulence factors of Vibrio and the related virulence genes of Vibiro are summarized, and their important roles in Vibrio infection are also discussed.
10
Chellapandian, Hethesh, Jeyachandran Sivakamavalli, A. Vijay Anand, and Balamuralikrishnan Balasubramanian. "Challenges in Controlling Vibriosis in Shrimp Farms." In Infectious Diseases and Sepsis [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97018.
Full textAbstract:
Recently the shrimp farming has blooming as a crucial counterpart in the aquaculture industry which contribute the remarkable role in sea food production as well economy of the country. However, this could be fluctuated every year through several circumstances such as unfavorable (Poor water and soil quality) environmental factors. The environmental factors includes disease causing bacterial pathogens in the soil and water which causes the bacterial diseases in the aquatic animals, like this hectic problems are prevented through bioaugmentation strategies. The pond environment plays a vital role in determining the healthy culture system, but there is high risk for manipulation by bacterial community which takes care of waste generated in the system through in situ bioremediation. Due to the impact of rapidly growing bacterial diseases of shrimps throughout the world, numerous studies have been carried out to find immunostimulants, immunomodulators and biotic component that can be used against vibrio causing pathogens, and can also be used as an alternative for antibiotics. Recent research focus towards the marine resources such as microalgae, seaweed, live feeds (like artemia, copepods, rotifers), bacteriophage, and probiotics have been found to have higher potential in reducing vibriosis. Eco-based shrimp farming includes green water technology, phage therapy bio-floc technology (BFT) and integrated multi-trophic aquaculture (IMTA), these methods hold a promising alternative to antibiotics in the near future. Bacterial diseases caused by vibrios have been reported in penaeid shrimp culture systems implicating at least 14 species and they are Vibrio harveyi, V. splendidus, V. parahaemolyticus, V. alginolyticus, V. anguillarum, V. vulnificuslogei etc.
Conference papers on the topic "Vibrio marinus"
1
Blanchet, D. "Vibro Acoustic Modeling of Marine Structures and Underwater Sound Radiation of Vibrating Structures." In 5th International Conference on Technology and Operation of Offshore Support Vessels. Singapore: Research Publishing Services, 2013. http://dx.doi.org/10.3850/978-981-07-7338-0_osv2013-15.
Full text
2
"Phenotypic characterization of marine phage cocktail from Batangas Philippines against Multi-Drug Resistant Pseudomonas aeruginosa, Methicillin Resistant Staphylococcus aureus, and Vibrio cholera." In Multi-Disciplinary Manila (Philippines) Conferences Jan. 23-24, 2017, Manila (Philippines). Universal Researchers (UAE), 2017. http://dx.doi.org/10.17758/uruae.ae0117608.
Full text