Journal articles: 'Vibrio marinus'

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Urakawa, Hidetoshi, Kumiko Kita-Tsukamoto, and Kouichi Ohwada. "Restriction fragment length polymorphism analysis of psychrophilic and psychrotrophic Vibrio and Photobacterium from the north-western Pacific Ocean and Otsuchi Bay, Japan." Canadian Journal of Microbiology 45, no. 1 (January 1, 1999): 67–76. http://dx.doi.org/10.1139/w98-128.

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Typing and identification of 60 marine psychrophilic and psychrotrophic vibrios isolated from the north-western Pacific Ocean and coastal environment of Japan were performed by restriction fragment length polymorphism analysis on the basis of polymerase chain reaction amplified 16S rDNA. We obtained 15 operational taxonomic units (OTUs) by digestion with four restriction endonucleases (HhaI, DdeI, RsaI, and Sau3AI); four large groups were obtained from the neighbor-joining method. Significant differences were observed in OTU composition between isolates from the deep sea and coastal areas. Vibrio marinus and Photobacterium species were the dominant culturable vibrios in the deep sea areas, while Vibrio splendidus like species were the dominant culturable vibrios in a coastal area of Japan.Key words: restriction analysis, Vibrio, Photobacterium, Vibrio marinus (Moritella marina), Vibrio splendidus.

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Tall, B. D., J. F. La Peyre, J. W. Bier, M. D. Miliotis, D. E. Hanes, M. H. Kothary, D. B. Shah, and M. Faisal. "Perkinsus marinus Extracellular Protease Modulates Survival of Vibrio vulnificus in Eastern Oyster (Crassostrea virginica) Hemocytes." Applied and Environmental Microbiology 65, no. 9 (September 1, 1999): 4261–63. http://dx.doi.org/10.1128/aem.65.9.4261-4263.1999.

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ABSTRACT The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificusin oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced byP. marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues.

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BAUMEISTER, LESLIE, MONA E. HOCHMAN, JOHN R. SCHWARZ, and ROBIN BRINKMEYER. "Occurrence of Vibrio vulnificus and Toxigenic Vibrio parahaemolyticus on Sea Catfishes from Galveston Bay, Texas." Journal of Food Protection 77, no. 10 (October 1, 2014): 1784–86. http://dx.doi.org/10.4315/0362-028x.jfp-14-175.

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Dorsal and pectoral fin spines from two species of sea catfishes (Bagre marinus and Ariopsis felis) landed at 54 sites in Galveston Bay, Texas, and its subbays from June to October 2005 were screened with traditional cultivation-based assays and quantitative PCR assays for Vibrio vulnificus and Vibrio parahaemolyticus. V. vulnificus was present on 51.2% of fish (n = 247), with an average of 403 ±337 SD cells g−1. V. parahaemolyticus was present on 94.2% (n = 247); 12.8% tested positive for the virulence-conferring tdh gene, having an average 2,039 ± 2,171 SD cells g−1. The increasing trend in seafood consumption of “trash fishes” from lower trophic levels, such as sea catfishes, warrants evaluation of their life histories for association with pathogens of concern for human consumption.

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Langridge, Patricia, R. D. Haight, and R. Y. Morita. "Use of heat for obtaining malic dehydrogenase from cells of Vibrio marinus." Zeitschrift für allgemeine Mikrobiologie 8, no. 3 (January 24, 2007): 221–23. http://dx.doi.org/10.1002/jobm.19680080308.

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Masuda, K. V., and L. J. Albright. "Hydrostatic pressure effects upon cellular leakage and active transport by Vibrio marinus." Zeitschrift für allgemeine Mikrobiologie 18, no. 10 (January 24, 2007): 731–40. http://dx.doi.org/10.1002/jobm.19780181005.

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Tokárová, Viola, Ayyappasamy Sudalaiyadum Perumal, Monalisha Nayak, Henry Shum, Ondřej Kašpar, Kavya Rajendran, Mahmood Mohammadi, et al. "Patterns of bacterial motility in microfluidics-confining environments." Proceedings of the National Academy of Sciences 118, no. 17 (April 19, 2021): e2013925118. http://dx.doi.org/10.1073/pnas.2013925118.

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Understanding the motility behavior of bacteria in confining microenvironments, in which they search for available physical space and move in response to stimuli, is important for environmental, food industry, and biomedical applications. We studied the motility of five bacterial species with various sizes and flagellar architectures (Vibrio natriegens, Magnetococcus marinus, Pseudomonas putida, Vibrio fischeri, and Escherichia coli) in microfluidic environments presenting various levels of confinement and geometrical complexity, in the absence of external flow and concentration gradients. When the confinement is moderate, such as in quasi-open spaces with only one limiting wall, and in wide channels, the motility behavior of bacteria with complex flagellar architectures approximately follows the hydrodynamics-based predictions developed for simple monotrichous bacteria. Specifically, V. natriegens and V. fischeri moved parallel to the wall and P. putida and E. coli presented a stable movement parallel to the wall but with incidental wall escape events, while M. marinus exhibited frequent flipping between wall accumulator and wall escaper regimes. Conversely, in tighter confining environments, the motility is governed by the steric interactions between bacteria and the surrounding walls. In mesoscale regions, where the impacts of hydrodynamics and steric interactions overlap, these mechanisms can either push bacteria in the same directions in linear channels, leading to smooth bacterial movement, or they could be oppositional (e.g., in mesoscale-sized meandered channels), leading to chaotic movement and subsequent bacterial trapping. The study provides a methodological template for the design of microfluidic devices for single-cell genomic screening, bacterial entrapment for diagnostics, or biocomputation.

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Birkbeck, T. H., B. Billcliffe, A. Laidler, and D. I. Cox. "The relationship between Aeromonas sp. NCIMB 2263, a causative agent of skin lesions in Atlantic salmon, Vibrio marinus (Moritella marina) and Vibrio viscosus." Journal of Fish Diseases 23, no. 4 (July 2000): 281–83. http://dx.doi.org/10.1046/j.1365-2761.2000.00228.x.

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SOKOLOVA, INNA M., JAMES D. OLIVER, and LARRY J. LEAMY. "AN AFLP APPROACH TO IDENTIFY GENETIC MARKERS ASSOCIATED WITH RESISTANCE TO VIBRIO VULNIFICUS AND PERKINSUS MARINUS IN EASTERN OYSTERS." Journal of Shellfish Research 25, no. 1 (April 2006): 95–100. http://dx.doi.org/10.2983/0730-8000(2006)25[95:aaatig]2.0.co;2.

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Ben Dhia Thabet, O., M. L. Fardeau, C. Suarez-Nuñez, M. Hamdi, P. Thomas, B. Ollivier, and D. Alazard. "Desulfovibrio marinus sp. nov., a moderately halophilic sulfate-reducing bacterium isolated from marine sediments in Tunisia." International Journal of Systematic and Evolutionary Microbiology 57, no. 9 (September 1, 2007): 2167–70. http://dx.doi.org/10.1099/ijs.0.64790-0.

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Two novel sulfate-reducing bacterial strains, designated E-2T and IMP-2, were isolated from geographically distinct locations. Strain E-2T was recovered from marine sediments near Sfax (Tunisia), whereas strain IMP-2 originated from oilfield production fluids in the Gulf of Mexico. Cells were Gram-negative, non-sporulated, motile, vibrio-shaped or sigmoid. They were strictly anaerobic, mesophilic and moderately halophilic. Sulfate, sulfite, thiosulfate and elemental sulfur served as electron acceptors, but not nitrate or nitrite. H2 (with acetate as carbon source), formate, fumarate, lactate, malate, pyruvate, succinate and fructose were used as electron donors in the presence of sulfate as terminal electron acceptor. Lactate was oxidized incompletely to acetate. Fumarate and pyruvate were fermented. Desulfoviridin and c-type cytochromes were present. 16S rRNA gene sequence analysis of the two strains showed that they were phylogenetically similar (99.0 % similarity) and belonged to the genus Desulfovibrio, with Desulfovibrio indonesiensis and Desulfovibrio gabonensis as their closest phylogenetic relatives. The G+C content of the DNA was respectively 60.4 and 62.7 mol% for strains E-2T and IMP-2. DNA–DNA hybridization experiments revealed that the novel strains had a high genomic relatedness, suggesting that they belong to the same species. We therefore propose that the two isolates be affiliated to a novel species of the genus Desulfovibrio, Desulfovibrio marinus sp. nov. The type strain is strain E-2T (=DSM 18311T =JCM 14040T).

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Hernández Mendoza, Dulce Maripaz, Pablo San Martín del Ángel, Catya Jiménez Torres, and Rosa Idalia Hernández Herrera. "Monitoreo de vibrio spp. en ostiones Crassostrea virginica de las lagunas de Tamiahua y Tampamachoco, Veracruz, México." Revista Biológico Agropecuaria Tuxpan 9, no. 1 (July 1, 2021): 122–41. http://dx.doi.org/10.47808/revistabioagro.v9i1.346.

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Vibrio es un género ampliamente distribuído en ambientes estuarinos y marinos, adaptable y con rápida propagación de sus cepas. Los moluscos bivalvos son reservorios potenciales de Vibrios, su consumo está relacionado con el desarrollo de vibriosis leve como gastroenteritis hasta septicemia grave o la muerte. Por lo cual es importante el monitoreo continuo en las áreas de extraccion y distribución para evaluar el riesgo para la salud humana derivado del consumo de moluscos y garantizar la inocuidad de estos bivalvos. En el presente estudio se determinaron las diferencias estacionales en la presencia y abundancia de Vibrios durante las fases de recolección (bancos ostrícolas) y post-recolección (cooperativas y restaurantes) en ostiones Crassostrea virginica de las lagunas de Tamiahua y Tampamachoco, Veracruz, México. Las muestras fueron obtenidas de trece sitios al azar durante el año 2018. El aislamiento bacteriano se realizó por el método de siembra por dilución en medio TCBS y recuento en placa de acuerdo a la NOM-031-SSA1-1993. El análisis estadístico indicó que no existieron diferencias espaciales significativas, no obstante, se presentaron diferencias estacionales. En esta investigación, se evidencia por primera vez, la presencia de Vibrio spp. en ostiones de las cooperativas de estudio. También se reporta la ausencia de este género bacteriano en los restaurantes adyacentes. En general, los resultados muestran la necesidad de aplicar medidas dirigidas a reducir la presencia de Vibrios durante la fase de post-recolección de ostiones, con la finalidad de disminuir el riesgo de infección por Vibrio spp. en los consumidores.

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Urakawa, H. "Corrigendum to ''A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov.'' [FEMS Microbiol. Lett. 165 (1998) 373â378]." FEMS Microbiology Letters 169, no. 1 (December 1, 1998): 207. http://dx.doi.org/10.1016/s0378-1097(98)00460-1.

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De la Fuente, Mery, and Víctor Faúndez. "Evaluación in vitro de bacterias marinas para potencial biocontrol en cultivo de moluscos bivalvos." Revista de Biología Marina y Oceanografía 54, no. 3 (January 17, 2020): 272. http://dx.doi.org/10.22370/rbmo.2019.54.3.2010.

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El cultivo de moluscos bivalvos en sistemas controlados requiere un adecuado control de mortalidades en estados larvales debido a infecciones bacterianas. En este trabajo se investigó la actividad de bacterias marinas provenientes de zonas costeras de la provincia de Arauco, Chile para ser usadas como potencial biocontrolador en sistemas artificiales de cultivo. Se realizó el muestreo de diferentes matrices comúnmente asociadas a moluscos bivalvos en su estado natural y se procedió al aislamiento de bacterias marinas. Se evaluó la capacidad de estas bacterias para inhibir 3 bacterias patógenas asociadas normalmente al cultivo de moluscos bivalvos, Vibrio anguillarum, Vibrio splendidus y Vibrio parahaemolyticus. Además, se realizó una caracterización fenotípica de las cepas con actividad inhibitoria, considerando sus propiedades de inocuidad y crecimiento, para la utilización práctica de estas cepas en sistemas de cultivo. De las 70 bacterias aisladas, 3 cepas fueron capaces de inhibir al menos un patógeno bacteriano, logrando crecer en agua marina. La caracterización fenotípica de estas 3 cepas permitió catalogarlas como pertenecientes a los géneros Vibrio y Pseudomonas. Una cepa marina fue totalmente inocua según las características evaluadas in vitro. Por tanto, es factible seleccionar bacterias con propiedades inhibitorias y de inocuidad para un potencial biocontrol en cultivos de moluscos bivalvos en la provincia de Arauco.

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OLIVER, J. D. "Wound infections caused by Vibrio vulnificus and other marine bacteria." Epidemiology and Infection 133, no. 3 (March 1, 2005): 383–91. http://dx.doi.org/10.1017/s0950268805003894.

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Infections caused by Vibrio vulnificus were first reported in 1979 by Blake et al. of the US Centers for Disease Control. At that time described as a ‘rare, unnamed halophilic lactose-fermenting Vibrio species’, V. vulnificus has emerged as the most virulent foodborne pathogen in the United States with a hospitalization rate of 0·910 and a case-fatality rate of 0·390. It is in addition a significant cause of potentially life-threatening wound infections. Infections following ingestion of raw or undercooked seafood, commonly raw oysters, can lead to a primary septicaemia with a fatality rate of 50–60%. An unusual symptom, occurring in 69% of 274 cases reviewed by Oliver, is the development of secondary lesions, typically on the extremities, which are generally severe (often a necrotizing fasciitis) and require tissue debridement or amputation. These cases occur almost exclusively in males over the age of 50 years. Interestingly, this gender specificity has been found to be due to the female hormone oestrogen, which in some manner provides protection against the lethal V. vulnificus endotoxin. Further, most cases occur in persons with certain underlying diseases which are either immunocompromising or which lead to elevated serum iron levels (e.g. liver cirrhosis, chronic hepatitis, haemochromatosis). V. vulnificus infections resulting in primary septicaemia have been extensively studied, and the subject of several reviews. This review concentrates on the wound infections caused by this marine bacterial pathogen, including the more recently described biotypes 2 and 3, with brief discussions of those caused by other marine vibrios, and the increasingly reported wound/skin infections caused by Mycobacterium marinum, Erysipelothrix rhusiopathiae, and Aeromonas hydrophila.

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Urdaci, M. C., M. Marchand, and P. A. D. Grimont. "Espèces du genre vibrio associées aux produits marins du bassin d'arcachon." Annales de l'Institut Pasteur / Microbiologie 139, no. 3 (May 1988): 351–62. http://dx.doi.org/10.1016/0769-2609(88)90027-0.

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Rojas-Alfaro, Rebeca, Rodolfo Umaña-Castro, Norman Rojas-Campos, and Maribel Vargas-Montero. "Primer reporte de bacterias y dinoflagelados marinos luminiscentes del Parque Nacional Isla del Coco, Costa Rica." Revista de Biología Tropical 68, S1 (March 24, 2020): S213—S224. http://dx.doi.org/10.15517/rbt.v68is1.41182.

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Introducción: La bioluminiscencia es la capacidad de ciertos organismos para transformar la energía química en energía lumínica mediante varios procesos bioquímicos. Objetivo: el aislamiento e identificación por primera vez de bacterias luminiscentes en agua marina superficial y la identificación de dinoflagelados luminiscentes marinos del Parque Nacional Isla del Coco, Costa Rica. Metodología: Se colectaron muestras de agua marina obtenida por buceo a 20 m y a nivel superficial de 13 sitios en la Isla del Coco, Costa Rica. Por otra parte, se analizaron muestras de fitoplancton colectadas desde la superficie hasta los 30 m de profundudad en los alrededores de 8 sitios de la Isla del Coco, y se determinaron varias especies luminiscentes pertenecientes a los géneros Ornithocercus y Ceratocorys. Resultados: Se logró obtener 7 aislados bacterianos luminiscentes, se identificaron y caracterizaron bioquímicamente mediante una plataforma automatizada (Vitek) con altos niveles de confianza, se ubicaron taxonómicamente dentro del género Vibrio, 2 especies: V. alginolyticus y V. parahaemolyticus, además, algunos aislados presentaron resistencia al antibiótico ampicilina y 100% capacidad hemolítica. Esta investigación muestra evidencia de la presencia de especies microscópicas marinas en Isla del Coco, Costa Rica, capaces de presentar el fenómeno de la luminiscencia, por lo que profundizar en su estudio sería relevante en cuanto a la importancia de estos microorganismos en la producción de metabolitos secundarios y como indicadores de floraciones algales nocivas, por lo que se hace necesario realizar más investigación científica para determinar su potencial biotecnológico. Conclusiones: De la misma forma, los resultados obtenidos en esta investigación sugieren expandir las localidades de colecta y aislamientos de microorganismos luminiscentes, acompañado de una caracterización bioquímica y molecular, con el fin de explorar la diversidad microbiana asociada a eventos de luminiscencia y determinar los ambientes en el que estas especies se desarrollan.

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Caro-Castro, Junior, Orson Mestanza, Willi Quino, and Ronnie G. Gavilán. "Diversidad molecular de variantes patogénicas de Vibrio parahaemolyticus en el Perú." Revista Peruana de Medicina Experimental y Salud Pública 37, no. 2 (June 1, 2020): 270–5. http://dx.doi.org/10.17843/rpmesp.2020.372.4984.

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Con el objetivo de determinar la diversidad de variantes patogénicas de Vibrio parahaemolyticus en el Perú durante el periodo 1995-2017, se analizaron 102 genomas peruanos (97 clínicos y 5 ambientales) empleando el esquema de tipificación multilocus y BLASTn para la búsqueda de genes de virulencia. Se identificaron 15 tipos de secuencia diferentes, encontrándose que el genotipo ST3, perteneciente al clon pandémico, fue el más abundante, con 52% (n=53); seguido por el ST120, con 23,5% (n=24); y el complejo clonal CC345, con 11,8% (n=12). Un total de 89 cepas analizadas presentaron genes que codifican la isla de patogenicidad VpaI-7 (87,3%), mientras que 96 presentaron el gen tdh (94,1%), y 6, el trh (5,9%). Durante el periodo evaluado, se resalta la predominancia del ST3, causante de un importante brote en el pasado del Perú, además de otros genotipos patógenos que representan un riesgo latente en salud pública asociado al consumo de alimentos marinos.

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Peralta Otero, Robert, Tessy Peralta Ortiz, and Alberto Ordinola-Zapata. "Characterization of oil bioremediating strains of Vibrio spp. isolated from seawater." Manglar 18, no. 1 (March 26, 2021): 21–26. http://dx.doi.org/10.17268/manglar.2021.003.

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SAULNIER, D., Y. REYNAUD, I. ARZUL, L. MIOSSEC, F. LE ROUX, and C. GOARANT. "Émergence de maladies chez les organismes d’intérêt aquacole : quelques scénarios illustrés d’exemples." INRAE Productions Animales 20, no. 3 (September 7, 2007): 207–12. http://dx.doi.org/10.20870/productions-animales.2007.20.3.3456.

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Selon l’Office International des Epizooties (OIE) une maladie émergente désigne une maladie grave récemment reconnue, dont la cause peut, ou non, avoir déjà été établie, et qui est susceptible de se propager au sein d’une population ou entre des populations, par exemple à l’occasion d’échanges internationaux d’animaux aquatiques et/ou de produits d’animaux aquatiques. Si les maladies émergentes qui affectent la santé humaine ont été très étudiées, celles qui touchent les organismes marins et les organismes aquacoles d’intérêt économique en particulier sont en revanche peu documentées. C’est en restreignant l’émergence aux seules maladies infectieuses que seront présentés de façon non exhaustive quelques scénarios de l’émergence des maladies chez les organismes d’intérêt aquacole en les illustrant par trois exemples disponibles dans la littérature scientifique : l’un relatif à l’apparition d’un agent pathogène chez un nouvel hôte avec le cas de l’herpesvirus de la carpe Koï, l’autre à l’évolution d’un agent pathogène existant avec le cas de la vibriose à Vibrio nigripulchritudo sévissant dans les élevages de crevettes pénéides de Nouvelle-Calédonie et enfin le dernier lié à l’introduction d’un pathogène préexistant avec le cas de Bonamia ostreae infectant l’huître plate Ostrea edulis. Les causes d’émergence de maladies sont multiples et font intervenir de façon intercurrente l’agent pathogène, l’environnement, l’hôte ou les espèces hôtes et des facteurs anthropiques. Dans le milieu marin, ces causes sont bien souvent méconnues. Dans ce contexte le développement des réseaux de surveillance et des techniques de diagnostic revêtent un intérêt considérable afin d’anticiper, de prévenir et/ou d’intervenir sur l’émergence des maladies en limitant leur conséquences sanitaires, écologiques et politiques.

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Jacobs, R., A. Decostere, and A. M. Declercq. "Bacteriële zoönotische agentia afkomstig van vissen." Vlaams Diergeneeskundig Tijdschrift 85, no. 3 (June 29, 2016): 115–23. http://dx.doi.org/10.21825/vdt.v85i3.16337.

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Het belang van zoönosen neemt de laatste jaren toe, ondermeer ten gevolge van de internationalisering van handel. Zoönosen kunnen via opname van voedsel (voedselzoönose) of via huidcontact (contactzoönose) optreden. Specifiek voor de toename van viszoönosen is de uitbreiding van de aquacultuur. Voornamelijk vissers en vishandelaars maar ook vishobbyisten lopen het grootste risico om aan vis, als mogelijke zoönotische infectiebron, te worden blootgesteld. Vaak zijn zij zich daar onvoldoende van bewust. Voorzorgsmaatregelen tegen zoönotische infecties zijn dan ook cruciaal. De belangrijkste bacteriële contactzoönosen van vis worden veroorzaakt door Mycobacterium marinum, Vibrio vulnificus, Edwardsiella tarda en Streptococcus iniae. Minder bedreigend zijn onder andere Aeromonas hydrophila en Erysipelothrix rhusiopathiae. Infectie van de mens gebeurt via contact van huidwonden met de bacterie en voor sommige van de genoemde bacteriën ook via orale weg. De diagnose van deze infecties kan meestal worden vermoed op basis van de anamnese waaruit contact met vissen blijkt. De diagnose van de specifieke kiem kan worden bevestigd met behulp van bacterie-isolatie en –identificatie, bijvoorbeeld via “polymerase chain reaction”. De behandeling van de infectie geschiedt meestal met antibiotica. Soms is tevens een chirurgische behandeling vereist.

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Oberbeckmann, Sonja, and Matthias Labrenz. "Marine Microbial Assemblages on Microplastics: Diversity, Adaptation, and Role in Degradation." Annual Review of Marine Science 12, no. 1 (January 3, 2020): 209–32. http://dx.doi.org/10.1146/annurev-marine-010419-010633.

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We have known for more than 45 years that microplastics in the ocean are carriers of microbially dominated assemblages. However, only recently has the role of microbial interactions with microplastics in marine ecosystems been investigated in detail. Research in this field has focused on three main areas: ( a) the establishment of plastic-specific biofilms (the so-called plastisphere); ( b) enrichment of pathogenic bacteria, particularly members of the genus Vibrio, coupled to a vector function of microplastics; and ( c) the microbial degradation of microplastics in the marine environment. Nevertheless, the relationships between marine microorganisms and microplastics remain unclear. In this review, we deduce from the current literature, new comparative analyses, and considerations of microbial adaptation concerning plastic degradation that interactions between microorganisms and microplastic particles should have rather limited effects on the ocean ecosystems. The majority of microorganisms growing on microplastics seem to belong to opportunistic colonists that do not distinguish between natural and artificial surfaces. Thus, microplastics do not pose a higher risk than natural particles to higher life forms by potentially harboring pathogenic bacteria. On the other hand, microplastics in the ocean represent recalcitrant substances for microorganisms that are insufficient to support prokaryotic metabolism and will probably not be microbially degraded in any period of time relevant to human society. Because we cannot remove microplastics from the ocean, proactive action regarding research on plastic alternatives and strategies to prevent plastic entering the environment should be taken promptly.

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Monsreal, José Franco, Lidia Esther del Socorro Serralta-Peralta, José Ricardo Hernández Gómez, Florinda Sosa-Castilla, and Juan Ariel Castillo-Cocom. "Prevalence of clinically important species of the genus Vibrio in catered seafood of city and port of Progreso de Castro, Yucatan, Mexico." Medwave 15, no. 05 (June 5, 2015): e6147-e6147. http://dx.doi.org/10.5867/medwave.2015.05.6147.

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Marín -Álvarez, A., J. I. Murrillo -Álvarez, M. Muñoz -Ochoa, and G. M. Molina -Salinas. "CHEMICAL CONSTITUENTS AND BIOACTIVITY OF Codium amplivesiculatum SETCHELL <&> N. L.GARDNER (CHLOROPHYTA; BRYOPSIDALES)." CICIMAR Oceánides 28, no. 2 (December 31, 2013): 1. http://dx.doi.org/10.37543/oceanides.v28i2.124.

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In search of bioactive substances from Mexican marine organisms, crude ethanol-extract from the marine alga Codium amplivesiculatum was fractionated in chromatographic columns of silica gel at 60 Å (230-400 mesh) using solvent mixtures of increasing polarity. All the fractions were submitted to antibacterial assays. The major metabolite from an anti-tuberculosis fraction (MIC = 100 μg mL–1) was purified and identified as 1-octodecanol (1). The anti-tuberculosis activity was attributed to 1 with bases in previous reports. In addition, clerosterol (2) was obtained by crystallization from an active fraction against Staphylococcus aureus and Vibrio parahaemolyticus (MIC = 125 and 250 μg mL–1, respectively). Both structures were established by interpretation and comparison of infrared and 1H NMR spectroscopic data. In contrast with other studies, 2 showed a non-significant cytotoxicity against the cell line PC-3 (% GI = 21.05 ± 0.3 at 50 μg mL–1). To our knowledge, these metabolites are reported for the first time from C. amplivesiculatum, and this is one of very rare reports of saturated long-chain alcohols isolated from chlorophytes. Constituyentes químicos y bioactividad de Codium amplivesiculatum Con el propósito de descubrir sustancias bioactivas a partir de organismos marinos encontrados en México, se fraccionó el extracto crudo etanólico de Codium amplivesiculatum en columnas cromatográficas de sílica gel 60 Å (230-400 de malla) utilizando mezclas de solventes de polaridad creciente. Todas las fracciones se sometieron a ensayos antibacterianos. El principal metabolito de la fracción activa antituberculosis (MIC = 100 μg mL-1), fue purificado e identificado como 1-octodecanol (1). La actividad antituberculosis, basada en reportes previos, se atribuyó al compuesto 1. Además, se obtuvo clerosterol (2) por cristalización de una fracción activa frente a Staphylococcus aureus y Vibrio parahaemolyticus (MIC = 125 y 250 μg mL-1, respectivamente). Las dos estructuras fueron inferidas mediante interpretación y comparación de datos obtenidos por espectroscopía de IR-ATR y 1H RMN. En contraste con otros estudios, el compuesto 2 mostró una citotoxicidad no significativa contra la línea celular PC-3 (% IC = 21.05 ± 0.3 a 50 μg mL–1). Hasta donde sabemos, estos metabolitos se reportan por primera vez en C. amplivesiculatum y 1-octadecanol es un reporte muy raro de alcohol de cadena larga aislado de clorofitas.

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Orozco, Rita, Alberto Lorenzo, Sonia Sánchez, Georgina Flores, Víctor Rebaza, and Nancy Blas. "Efectos del agua de lastre sobre la calidad acuática de las zonas Portuarias de Callao, Pisco, Paita y Salaverry, Perú." Revista del Instituto de Investigación de la Facultad de Ingeniería Geológica, Minera, Metalúrgica y Geográfica 23, no. 46 (November 30, 2020): 55–64. http://dx.doi.org/10.15381/iigeo.v23i46.19183.

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El crecimiento de la industria naviera en el Perú y la ampliación de la zona portuaria del Callao, Pisco, Paita y Salaverry, permiten el ingreso de buques de gran calado. En los años 2009-2010, se reportó el ingreso de especies invasoras a través del agua de lastre en el Callao. Se realizó una evaluación del agua de lastre en los buques que arribaron al puerto del Callao en los años 2010 y 2012.también se evaluó la calidad acuática de los puertos de Paita Salaverry, Callao y Pisco entre 2011 y 2013. Los resultados muestran que en el agua de lastre los recuentos de coliformes fueron bajos de < 2 a 8 NMP/100ml, pero se registró a las bacterias Vibrio parahaemolyticus y V. vulnificus y especies fitoplanctónicas como Heterosigma akashivo y, Skeletonema costatum sp fueron las más representativas. Las aguas marinas de los puertos tenían valores normales de temperatura <20 ° C y salinidades > 35.1 ups, pero la calidad acuática fue afectada por actividades antropogénicas con valores elevados de coliformes termotolerantes en el Callao de 1,6x106 NMP/100ml. Las especies de fitoplancton dominantes producen floraciones algales Dictyocha fibula y Prorocentrum micans en Paita, Prorocentrum minimun, A. sanguineum en Salaverry en 2012, Heterosigma akashiwo y Akashiwo sanguinea,en el Callao en 2013. En el puerto de Pisco, las especies más abundantes fueron Cochlodinium polikrykoides en 2011 y Alexandrium peruvianum en mayo de 2012. El agua de lastre transporta especies exóticas invasoras que pueden causar graves efectos en el ecosistema marino, la salud humana y la economía, por lo cual debe fortalecerse el monitoreo de puertos de mayor tráfico marítimo.

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Kojima, Masaru, Igor B. Zhulin, Michio Homma, and Ikuro Kawagishi. "2P321 Distinct features of the bidirectional and unidirectional flagellar motors of marine Vibrio(42. Sensory signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)." Seibutsu Butsuri 46, supplement2 (2006): S376. http://dx.doi.org/10.2142/biophys.46.s376_1.

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Nowik, N., P. Podlasz, A. Jakimiuk, N. Kasica, W. Sienkiewicz, and J. Kaleczyc. "Zebrafish: an animal model for research in veterinary medicine." Polish Journal of Veterinary Sciences 18, no. 3 (September 1, 2015): 663–74. http://dx.doi.org/10.1515/pjvs-2015-0086.

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Abstract The zebrafish (Danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. Nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. Comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their development, which makes possible to simplify the research techniques such as fate mapping, fluorescent tracer time-lapse lineage analysis and single cell transplantation. Although zebrafish are only 2.5 cm long, they are easy to maintain. Intraperitoneal and intracerebroventricular injections, blood sampling and measurement of food intake are possible to be carry out in adult zebrafish. Danio rerio is a useful animal model for neurobiology, developmental biology, drug research, virology, microbiology and genetics. A lot of diseases, for which the zebrafish is a perfect model organism, affect aquatic animals. For a part of them, like those caused by Mycobacterium marinum or Pseudoloma neutrophila, Danio rerio is a natural host, but the zebrafish is also susceptible to the most of fish diseases including Itch, Spring viraemia of carp and Infectious spleen and kidney necrosis. The zebrafish is commonly used in research of bacterial virulence. The zebrafish embryo allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host. Plenty of common pathogens can be examined using zebrafish model: Streptococcus iniae, Vibrio anguillarum or Listeria monocytogenes. The steps are taken to use the zebrafish also in fungal research, especially that dealing with Candida albicans and Cryptococcus neoformans. Although, the zebrafish is used commonly as an animal model to study diseases caused by external agents, it is also useful in studies of metabolic disorders including fatty liver disease and diabetes. The zebrafish is also a valuable tool as a model in behavioral studies connected with feeding, predator evasion, habituation and memory or lateralized control of behavior. The aim of the present article is to familiarize the reader with the possibilities of Danio rerio as an experimental model for veterinary medicine.

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Walther, Benjamin D., Paxton T. Bachand, Andrew Hinson, Colin A. O’Donnell, and Jeffrey W. Turner. "Investigating reconstructed inflows and pathogen infection patterns between natural and restored subtropical oyster reefs." Experimental Results 1 (2020). http://dx.doi.org/10.1017/exp.2020.28.

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AbstractFlood and drought events cause significant freshwater inflow fluctuations in estuaries, potentially leading to physiological stress and altered abundances of pathogens such as Vibrio vulnificus and Perkinsus marinus in oysters. To assess the effects of freshwater pulses to oyster reefs in subtropical estuaries in Texas, this study accomplished two goals: 1) reconstructed a reef-specific history of freshwater pulses through shell stable isotope analysis, 2) quantified the abundance of V. vulnificus and P. marinus through culture-dependent and culture-independent microbiology analyses. Oysters from a natural and restored reef experienced similar fluctuations in shell isotopes, indicating similar ranges of past environmental conditions. V. vulnificus and P. marinus were detected throughout the study but the abundance of these microorganisms was not correlated with environmental parameters or one another. Importantly, the P. marinus infection intensity was always lower at the restored reef, which suggests that restored reefs may experience lower infection frequencies.

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Rohomania, Tahara, Mihir Lal Saha, Anwar Hossain, and Mohammad Shamsur Rahman. "Morphological and Biochemical Characterization of Bacteria Isolated from Fresh and Salted Hilsa, Tenualosa ilisha (Hamilton, 1822)." Bangladesh Journal of Microbiology, June 25, 2016, 7–13. http://dx.doi.org/10.3329/bjm.v32i0.28471.

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Morphological and biochemical characterization of different bacteria isolated from eight fresh and salted hilsa collected from four fish markets namely Palashi Bazaar, Karwan Bazaar, Anando Bazaar and New Market of Dhaka city was carried out. Five types of bacteriological culture media namely nutrient agar as generalized complex medium, EMB agar medium for coliform, SS agar for Salmonella-Shigella, MSA for Staphylococcus and TCBS for Vibrio were used to isolate the bacteria associated with the hilsa. In salted hilsa, no microbial growth was observed on SS and TCBS agar plate. A total of 66 bacterial isolates were obtained in the study where detail study of 24 strains (12 Gram positive and 12 Gram negative) using morphological and biochemical characterization was conducted. Variable morphological and biochemical properties of the isolated strains were observed. Gram positive bacteria such as Listeria monocytogenes, Bacillus megaterium, B. marinus, Planococcus citreus, Micrococcus varians, M. nishinomiyaensis, Staphylococcus lentus, S. epidermidis, S. aureus and Gram negative bacteria such as Salmonella paratyphi, Salmonella spp., Proteus morganii, P. vulgaris, Vibrio sp., Halobacterium sp. were presumptively identified in the current study. Presence of these bacteria in fish indicates the possibility of occurrence of food poisoning. So, care should be taken during handling and consumption of hilsa whether fresh or salted as it carries pathogenic strains of bacteria of public health concern.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 7-13

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Leyton, Yanett, and Carlos Riquelme. "Vibrios en los sistemas marinos costeros." Revista de biología marina y oceanografía 43, no. 3 (December 2008). http://dx.doi.org/10.4067/s0718-19572008000300004.

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Osuntokun, Oludare Temitope, Adewole Adeyemo Muniru, and Komolafe Temitope Morenike. "Bacteriological Assessment of African Catfish (Clarias gariepinus) Isolated from Earthen and Concrete Fish Pond." Asian Journal of Biochemistry, Genetics and Molecular Biology, November 17, 2020, 17–34. http://dx.doi.org/10.9734/ajbgmb/2020/v6i130143.

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The purpose of this research work is to evaluates, isolate, identify, characterized and compare the bacteria load in African Catfish (Clarias gariepinus) from Earthen and Concrete Fish Pond. African Catfish (Clarias gariepinus) is a choice culture fish and an African delicacy to African consumers. Concrete pond and Earthen pond are the two types of ponds used in fish farming in West Africa. An earthen pond is a water body that is enclosed by earth while a concrete pond are pond constructed with bricks (plastered) or tanks. Clarias gariepinus were obtained from the earthen and concrete ponds from Adekunle Ajasin University, Akungba–Akoko, Ondo state, Nigeria. The fishes were harvested and eviscerated and different organs of the fish were collected for the purpose of this research work. Isolation of bacteria was done using the streaking method of cultural media. Preliminary characterization of bacterial isolates were based on Gram staining, morphological and cultural characteristics. Further characterization was carried out with various biochemical tests (Catalase, Citrate, Indole, Oxidase test, Starch hydrolysis, Urease and Sugar fermentation) and Bergey’s manual Microbiology. In concrete pond, it was observed that Bacillus subtilis was the most percentage frequently distributed bacteria isolate in Clarias gariepinus with (8%) Staphylococcus aureus (9.5%), Alcaligenes xylosidans (4.7%), Alcaligenes paradoxus (4.7%), Acinetobacter calcoa ceticus (4.7%), Pseudomonas putida (4.7%), Bacillus cereus (23.8%), Citrobacter amalonaticus (9.5%), Acinetobacter baumanni(4.7%), Listeria grayi(9.5%) and Listeria monocytogenes (4.7%) while In earthen pond Enterococcus gallinarum (4.0%), Streptococcus ub eris (8.0%) and Micrococcus luteus(4.0%) was the most frequently distributed bacteria isolate in Clarias gariepinus earthen pond, Marinococcus halophilus(4.0%),Enterobacter aerogenes (4.0%), Micrococcus lylae (4.0%), Alcalige nes faecalis(4.0%), Enterococcus molodoratus (4.0%), Enterococcus gallinarum (8.0%), Bacillus pumilus (4.0%), Citrobacter freundii (4.0%), Sporosa rcina inulinus (4.0%), Deinococcus radiodurans (4.0%), Vibrio marinus(4.0%), Listeria murrayi (4.0%), Deinobacter grandis(4.0%), Deinococcus proteolyticus (4.0%), Bacillus lautus(4.0%) and Micrococcus halobius (4.0%). Highest viable colony counts (5.6 × 104 for C. gariepinus were found in the concrete pond and (6.3 × 104) from the earthen pond respectively. Alimentary canal of fish in the concrete pond has the highest value of 4.73±0.81a and fish body has the lowest values (3.53±0.99a).Fish water has the highest value (4.33±1.15a) and lowest value (2.20±1.2a)) were found in earthen pond. It can be concluded that this organisms isolated from C. gariepinus in this study has the potential of becoming pathogenic and dangerous health risk and constitute severe economic loss to fish farmers and general populate especially those that consume catfish, therefore the Catfish should be raised in an hygienic and properly processed methods before consumption.

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Iglesias Rodríguez, María Victoria, Rodolfo Umaña Castro, Lirialis Garcia Mesa, Eudalys Ortiz Guilarte, Roberto Núñez Moreira, Carlos Álvarez Valcárcel, and Gladys Margarita Lugioyo Gallardo. "Caracterización fenotípica y molecular, e influencia de medios de cultivo, en el crecimiento y emisión de luz de bacterias del litoral de La Habana, Cuba." Revista de Biología Tropical 68, no. 4 (September 18, 2020). http://dx.doi.org/10.15517/rbt.v68i4.41269.

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Introducción: El complejo enzimático emisor de luz de las bacterias luminiscentes es una poderosa herramienta bioquímica, con una amplia variedad de aplicaciones, incluyendo el control de la calidad ambiental. Objetivos: Identificar taxonómicamente dos bacterias luminiscentes de las aguas de la plataforma cubana, así como seleccionar los medios de cultivo que favorezcan su crecimiento y luminiscencia. Métodos: La identificación taxonómica de las bacterias luminiscentes se llevó a cabo utilizando métodos tradicionales y moleculares. Cuatro medios de cultivo (LM, Boss, Chalk, ZoBell) fueron evaluados en función de la tasa de crecimiento específico (μ) y la luminiscencia utilizando un espectrofotómetro Genesys 10UV y un espectrofluorómetro Shimadzu RF-5301pc, respectivamente. Resultados: La caracterización bioquímica y fisiológica de los aislamientos de CBM-976 y CBM-992 mostró similitudes con las especies de Vibrio harveyi. El análisis del posicionamiento taxonómico confirmó una alta correspondencia con las cepas de V. harveyi aisladas de entornos acuáticos, utilizando secuencias parciales de los genes 16S rRNA, gyrB y pyrH. Se seleccionaron medios de cultivo LM y ZoBell por tener una alta tasa de crecimiento específico de las cepas CBM-976 y CBM-992; así como por mostrar altos valores de luminiscencia. Los resultados permitirán profundizar en la caracterización fisiológica y son el punto de partida para el desarrollo de métodos de detección de contaminantes. Conclusiones: La combinación de las características fisiológicas y bioquímicas, así como las técnicas de biología molecular contribuyeron a determinar la posición taxonómica de las cepas CBM-976 y CBM-992 aisladas de las aguas marinas cubanas como Vibrio harveyi. Además, se seleccionaron los medios de cultivo LM y ZoBell como los más adecuados para el crecimiento y la emisión de luminiscencia de ambas cepas.

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Vilcapoma, Amabilia, Abad Flores, Jorge León, and Débora Alvarado. "Determinación de la frecuencia de Vibrio parahaemolyticus y otros vibriones halofilicos en alimentos preparados con productos marinos frescos y procesados." Revista Peruana de Biología 4, no. 1-2 (June 9, 2014). http://dx.doi.org/10.15381/rpb.v4i1-2.8334.

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Bohle, H., F. Kjetil, P. Bustos, A. Riofrío, and C. Peters. "Fenotipo atípico de Vibrio ordalii, bacteria altamente patogénica aislada desde salmón del Atlántico cultivado en las costas marinas del sur de Chile." Archivos de medicina veterinaria 39, no. 1 (2007). http://dx.doi.org/10.4067/s0301-732x2007000100006.

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Journal articles on the topic 'Vibrio marinus'

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