To see the other types of publications on this topic, follow the link: Vibrissae hair follicle cells.
Journal articles on the topic 'Vibrissae hair follicle cells'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Vibrissae hair follicle cells.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Tang, Pei, Xueer Wang, Min Zhang, et al. "Activin B Stimulates Mouse Vibrissae Growth and Regulates Cell Proliferation and Cell Cycle Progression of Hair Matrix Cells through ERK Signaling." International Journal of Molecular Sciences 20, no. 4 (2019): 853. http://dx.doi.org/10.3390/ijms20040853.
Full textAbstract:
Activins and their receptors play important roles in the control of hair follicle morphogenesis, but their role in vibrissae follicle growth remains unclear. To investigate the effect of Activin B on vibrissae follicles, the anagen induction assay and an in vitro vibrissae culture system were constructed. Hematoxylin and eosin staining were performed to determine the hair cycle stages. The 5-ethynyl-2′-deoxyuridine (EdU) and Cell Counting Kit-8 (CCK-8) assays were used to examine the cell proliferation. Flow cytometry was used to detect the cell cycle phase. Inhibitors and Western blot analysis were used to investigate the signaling pathway induced by Activin B. As a result, we found that the vibrissae follicle growth was accelerated by 10 ng/mL Activin B in the anagen induction assay and in an organ culture model. 10 ng/mL Activin B promoted hair matrix cell proliferation in vivo and in vitro. Moreover, Activin B modulates hair matrix cell growth through the ERK–Elk1 signaling pathway, and Activin B accelerates hair matrix cell transition from the G1/G0 phase to the S phase through the ERK–Cyclin D1 signaling pathway. Taken together, these results demonstrated that Activin B may promote mouse vibrissae growth by stimulating hair matrix cell proliferation and cell cycle progression through ERK signaling.
2
Trivedi, Mahendra Kumar, and Snehasis Jana. "Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture." JOURNAL OF DERMATOLOGICAL RESEARCH AND THERAPY 1, no. 3 (2019): 12–19. https://doi.org/10.14302/issn.2471-2175.jdrt-18-2520.
Full textAbstract:
Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc. <strong>Source:</strong> https://www.trivedieffect.com/science/assessment-of-hair-growth-treatment-with-the-consciousness-energy-healing-treated-williams-medium-e-using-mouse-vibrissae-hair-follicle-organ-culture/ https://openaccesspub.org/jdrt/article/957
3
Reynolds, A. J., and C. A. Jahoda. "Cultured dermal papilla cells induce follicle formation and hair growth by transdifferentiation of an adult epidermis." Development 115, no. 2 (1992): 587–93. http://dx.doi.org/10.1242/dev.115.2.587.
Full textAbstract:
Adult rat pelage follicle dermal papilla cells induced follicle neogenesis and external hair growth when associated with adult footpad skin epidermis. They thus demonstrated a capacity to completely change the structural arrangement and gene expression of adult epidermis—an ability previously undocumented for cultured adult cells. Isolation chambers ensured that de novo follicle formation must have occurred by eliminating the possibility of cellular contributions, and/or inductive influences, from local skin follicles. These findings argue against previous suggestions of vibrissa follicle specificity, and imply that the potential for hair follicle induction may be common to all adult papilla cells.
4
Magnaldo, T., and Y. Barrandon. "CD24 (heat stable antigen, nectadrin), a novel keratinocyte differentiation marker, is preferentially expressed in areas of the hair follicle containing the colony-forming cells." Journal of Cell Science 109, no. 13 (1996): 3035–45. http://dx.doi.org/10.1242/jcs.109.13.3035.
Full textAbstract:
We have isolated, by subtractive and differential hybridization from a library constructed from keratinocyte colony-forming cells (K-CFCs), a cDNA coding for the rat CD24 (nectadrin, heat stable antigen). CD24, a glycoprotein thought to be involved in cell-cell adhesion and signalling, is highly expressed in keratinocytes located in the bulge area of the rat vibrissa which contains the most K-CFCs. CD24 is also expressed in the outer epithelial sheath of human hair follicles and in glabrous epidermis. However, its expression is not restricted to K-CFCs as demonstrated by cell sorting experiments, and it is thus not a specific marker of clonogenic keratinocytes. Rather, its preferential distribution in keratinocytes located in the most innervated area of the rat vibrissal follicle, i.e., the bulge, suggests that is function could be related to the tactile role of the hair follicle.
5
Trivedi, Mahendra Kumar, and Snehasis Jana. "Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture." Journal of Dermatologic Research And Therapy 1, no. 3 (2019): 12–19. http://dx.doi.org/10.14302/issn.2471-2175.jdrt-18-2520.
Full textAbstract:
Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc.
6
Zhang, Huishan, Shoubing Zhang, Huashan Zhao, et al. "Ovine Hair Follicle Stem Cells Derived from Single Vibrissae Reconstitute Haired Skin." International Journal of Molecular Sciences 16, no. 8 (2015): 17779–97. http://dx.doi.org/10.3390/ijms160817779.
Full text
7
Reynolds, A. J., and C. A. Jahoda. "Hair matrix germinative epidermal cells confer follicle-inducing capabilities on dermal sheath and high passage papilla cells." Development 122, no. 10 (1996): 3085–94. http://dx.doi.org/10.1242/dev.122.10.3085.
Full textAbstract:
Low passage cultured dermal papilla cells from adult rats stimulate complete hair follicle neogenesis when re-implanted into heterotypic skin. In contrast, cultured sheath cells are non-inductive despite sharing other behavioural characteristics (a common lineage and in situ proximity) with papilla cells. However, since sheath cells can behave inductively in amputated follicles after regenerating the papilla, this poses the question of what influences the sheath to papilla cell transition? During reciprocal tissue interactions specific epidermal cues are crucial to skin appendage development, and while in vivo assays to date have focussed on dermal interactive influence, our aim was to investigate epidermal potential. We have previously observed that hair follicle epidermal cells display exceptional interactive behaviour when combined with follicle dermal cells in vitro. Thus in the present study, hair follicle germinative, outer root sheath or skin basal epidermal cells were separately combined with each of three non-inductive dermal cell types (high passage papilla, low passage sheath or fibroblast) and then implanted into small ear skin wounds. The sheath/germinative and papilla/germinative cell implants repeatedly induced giant vibrissa-type follicles and fibres. In complete contrast, any single cell type and all other forms of recombination were consistently non-inductive. Hence, the adult germinative epidermal cells enable non-inductive adult dermal cells to stimulate hair follicle neogenesis, effectively, by altering their ‘status’, causing the sheath cells to ‘specialise’ and the ‘aged’ papilla cells to ‘rejuvenate’.
8
Chernova, O. F., V. F. Kulikov, and A. V. Abramov. "The hair structure of the long-eared gymnure (Otohylomys megalotis)." Proceedings of the Zoological Institute RAS 319, no. 3 (2015): 428–40. http://dx.doi.org/10.31610/trudyzin/2015.319.3.428.
Full textAbstract:
Weak degree of hair differentiation and microstructure of hair and whiskers of Otohylomys megalotis are similar to those in Hylomys suillus and Neotetracus sinensis: there are spear-shaped lead hairs and zigzag guard hairs of three orders, downy hairs are missing. In O. megalotis: 1) hairs are longer than those of other gymnures; 2) thin convoluted hair bases bound up contributing to the formation of the inert layer of air near the surface of the skin, improves the thermal insulation properties of hair in the absence of downy hairs; 3) strength in thinnest areas of the shaft (at its base and excesses) is provided by thickening of its cuticular scales, the special interconnection between cuticle and cortex, and cruciform layout of medulla discs in these places; 4) the pineal cuticular ornament of hair bases is characteristic of all three species of gymnures and resembles that of other insectivores, as well as of some marsupials and carnivores that reflects similar hair adaptations to the habitats; 5) for the first time discovered specialized pyramidal medulla of vibrissae, stiffening a shaft that is necessary for effective transfer of mechanical impulses to nerve cells of vibrissae follicle and functioning of whiskers as a tactile organ; 6) a long proboscis with well-developed nasal vibrissae and also numerous long whiskers on muzzle, neck, wrists and forearms are important and effective for the O. megalotis orientation in complex terrain karst habitats.
9
Yuen, Gary Ka-Wing, Bryan Siu-Yin Ho, Lish Sheng-Ying Lin, Tina Ting-Xia Dong, and Karl Wah-Keung Tsim. "Tectoridin Stimulates the Activity of Human Dermal Papilla Cells and Promotes Hair Shaft Elongation in Mouse Vibrissae Hair Follicle Culture." Molecules 27, no. 2 (2022): 400. http://dx.doi.org/10.3390/molecules27020400.
Full textAbstract:
To search hair growth-promoting herbal extract, a screening platform of having HEK293T fibroblast being transfected with pTOPFLASH DNA construct was developed over a thousand of herbal extracts and phytochemicals were screened. One of the hits was ethanolic extract of Rhizoma Belamcandae, the rhizome of Belamcanda chinensis (L.) DC. Tectoridin, an isoflavone from Rhizoma Belamcandae, was shown to be responsible for this activation of promoter construct, inducing the transcription of pTOPFLASH in the transfected fibroblasts in a dose-dependent manner. The blockage by DKK-1 suggested the action of tectoridin could be mediated by the Wnt receptor. The hair growth-promoting effects of tectoridin were illustrated in human follicular dermal papilla cells and mouse vibrissae organ cultures. In tectoridin-treated dermal papilla cultures, an activation of Wnt signaling was demonstrated by various indicative markers, including TCF/LEF1 transcriptional activity, nuclear translocation of β-catenin, expressions level of mRNAs encoding axin-related protein, (AXIN2), β-catenin, lymphoid enhancer-binding factor-1 (LEF-1), insulin-like growth factor 1 (IGF-1) and alkaline phosphatase (ALP). In addition, an increase of hair shaft elongation was observed in cultured mouse vibrissae upon the treatment of tectoridin. Tectoridin, as well as the herbal extract of Rhizoma Belamcandae, possesses hair promoting activity, which deserves further development.
10
Horne, K. A., and C. A. Jahoda. "Restoration of hair growth by surgical implantation of follicular dermal sheath." Development 116, no. 3 (1992): 563–71. http://dx.doi.org/10.1242/dev.116.3.563.
Full textAbstract:
The capacity of lower follicle dermal sheath to restore hair growth was tested by removing the lower halves of follicles, and then immediately implanting material containing dermal sheath cells from these bases, into the remaining upper epidermal follicle cavity. Over 60% of recipient follicles produced stout emergent vibrissa fibres and some operations resulted in multiple hair production from a single follicle. Histological examination revealed new dermal papillae within large bulb structures which were sited below the level of amputation--a feature that indicated that the new dermal papilla was derived from implanted material. For many follicles, the failure to produce emergent fibres could be accounted for after histological examination. These results provide clear evidence that lower follicle dermal sheath cells are capable of replacing those of the dermal papilla and it shows that they can do so in the context of the upper follicle. However, because elements of lower follicle epidermis were present in the implant material, the interactive sequence of events cannot be established. Dermal sheath cells have immense potential for papilla cell replacement: questions remain as to whether the distinction between sheath and papilla cells is one of context, or whether the transition requires specific external influences.
11
Rothnagel, J. A., and G. E. Rogers. "Trichohyalin, an intermediate filament-associated protein of the hair follicle." Journal of Cell Biology 102, no. 4 (1986): 1419–29. http://dx.doi.org/10.1083/jcb.102.4.1419.
Full textAbstract:
A precursor protein associated with the formation of the citrulline-containing intermediate filaments of the hair follicle has been isolated and characterized. The protein, with a molecular weight of 190,000, was isolated from sheep wool follicles and purified until it yielded a single band on a SDS polyacrylamide gel. The Mr 190,000 protein has a high content of lysine and glutamic acid/glutamine residues and is rich in arginine residues, some of which, it is postulated, undergo a side chain conversion in situ into citrulline residues. Polyclonal antibodies were raised to the purified protein, and these cross-react with similar proteins from extracts of guinea pig and human follicles and rat vibrissae inner root sheaths. Tissue immunochemical methods have localized the Mr 190,000 protein to the trichohyalin granules of the developing inner root sheath of the wool follicle. We propose that the old term trichohyalin be retained to describe this Mr 190,000 protein. Immunoelectron microscopy has located the Mr 190,000 protein to the trichohyalin granules but not to the newly synthesized filaments. This technique has revealed that trichohyalin becomes associated with the filaments at later stages of development. These results indicate a possible matrix role for trichohyalin.
12
Jahoda, C. A., A. J. Reynolds, C. Chaponnier, J. C. Forester, and G. Gabbiani. "Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro." Journal of Cell Science 99, no. 3 (1991): 627–36. http://dx.doi.org/10.1242/jcs.99.3.627.
Full textAbstract:
We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Dermal papilla cells within all types of follicles did not express the antigen. However, in culture a large percentage of both hair dermal papilla and dermal sheath cells were stained by this antibody. The same cells were negative when tested with an antibody to desmin. Overall, explant-derived skin fibroblasts had relatively low numbers of positively marked cells, but those from skin regions of high hair-follicle density displayed more smooth muscle alpha-actin expression than fibroblasts from areas with fewer follicles. 2-D SDS-PAGE confirmed that, unlike fibroblasts, cultured papilla cells contained significant quantities of the alpha-actin isoform. The rapid switching on of smooth muscle alpha-actin expression by dermal papilla cells in early culture, contrasts with the behaviour of smooth muscle cells in vitro, and has implications for control of expression of the antigen in normal adult systems. The very high percentage of positively marked cultured papilla and sheath cells also provides a novel marker of cells from follicle dermis, and reinforces the idea that they represent a specialized cell population, contributing to the heterogeneity of fibroblast cell types in the skin dermis, and possibly acting as a source of myofibroblasts during wound healing.
13
Wang, Caibing, Kai Zang, Zexin Tang, Ting Yang, Xiyun Ye, and Yongyan Dang. "Hordenine Activated Dermal Papilla Cells and Promoted Hair Regrowth by Activating Wnt Signaling Pathway." Nutrients 15, no. 3 (2023): 694. http://dx.doi.org/10.3390/nu15030694.
Full textAbstract:
Hordenine is effective in treating hyperpigmentation, fighting diabetes and resisting fibrosis and acute inflammation. However, the role of Hordenine on hair growth has not been elucidated. Here, we found that Hordenine treatments significantly enhance proliferation of primary mouse dermal-papilla cells (DPCs) and increase the activity of DPCs in a dose-dependent manner. Additionally, Hordenine markedly promoted the elongation of the hair shaft in the model of in vitro-cultured mouse vibrissa follicle and accelerated hair regrowth in a mouse model of depilation-induced hair regeneration. Real-time PCR, Western Blot and immunofluorescent assays showed that nuclear β-catenin and its downstream gene expression such as Lef1, Axin2, Cyclin D1 and ALP were greatly upregulated in DPCs and mouse hair follicles after Hordenine treatments. Moreover, the increased DPCs’ proliferation and hair shaft elongation of cultured mouse vibrissa follicles induced by Hordenine treatments were rescued by a Wnt/β-catenin signaling inhibitor, FH535. These data indicate that Hordenine can effectively enhance DPCs’ activity and accelerate hair regrowth through activating the Wnt/β-catenin signaling pathway. Therefore, these findings suggest Hordenine/its derivatives may be potentially used for preventing and treating alopecia in the future.
14
Yu, D. W., T. Yang, T. Sonoda, et al. "Message of nexin 1, a serine protease inhibitor, is accumulated in the follicular papilla during anagen of the hair cycle." Journal of Cell Science 108, no. 12 (1995): 3867–74. http://dx.doi.org/10.1242/jcs.108.12.3867.
Full textAbstract:
A group of specialized mesenchymal cells located at the root of the mammalian hair follicle, known as the follicular or dermal papillary cells, are involved in regulating the hair cycle, during which keratinocytes of the lower follicle undergo proliferation, degeneration and regrowth. Using the arbitrarily primed-PCR approach, we have identified a 1.3 kb messenger RNA that is present in large quantities in cultured rat follicular papillary cells, but not in skin fibroblasts. This mRNA encodes nexin 1, a potent protease inhibitor that can inactivate several growth-modulating serine proteases including thrombin, urokinase and tissue plasminogen activator. In situ hybridization showed that nexin 1 message is accumulated in the follicular papilla cells of anagen follicles, but is undetectable in keratinocytes or other skin mesenchymal cells. In addition, nexin 1 message level varies widely among several immortalized rat vibrissa papillary cell lines, and these levels correlate well with the reported abilities of these cell lines to support in vivo follicular reconstitution. These results suggest a possible role of nexin 1 in regulating hair follicular growth.
15
Manti, Pierluigi G., Fabrice Darbellay, Marion Leleu, et al. "The Transcriptional Regulator Prdm1 Is Essential for the Early Development of the Sensory Whisker Follicle and Is Linked to the Beta-Catenin First Dermal Signal." Biomedicines 10, no. 10 (2022): 2647. http://dx.doi.org/10.3390/biomedicines10102647.
Full textAbstract:
Prdm1 mutant mice are one of the rare mutant strains that do not develop whisker hair follicles while still displaying a pelage. Here, we show that Prdm1 is expressed at the earliest stage of whisker development in clusters of mesenchymal cells before placode formation. Its conditional knockout in the murine soma leads to the loss of expression of Bmp2, Shh, Bmp4, Krt17, Edar, and Gli1, though leaving the β-catenin-driven first dermal signal intact. Furthermore, we show that Prdm1 expressing cells not only act as a signaling center but also as a multipotent progenitor population contributing to the several lineages of the adult whisker. We confirm by genetic ablation experiments that the absence of macro vibrissae reverberates on the organization of nerve wiring in the mystacial pads and leads to the reorganization of the barrel cortex. We demonstrate that Lef1 acts upstream of Prdm1 and identify a primate-specific deletion of a Lef1 enhancer named Leaf. This loss may have been significant in the evolutionary process, leading to the progressive defunctionalization and disappearance of vibrissae in primates.
16
Woo, Jiwon, Wonhee Suh, and Jonghyuk Sung. "Hair Growth Regulation by Fibroblast Growth Factor 12 (FGF12)." International Journal of Molecular Sciences 23, no. 16 (2022): 9467. http://dx.doi.org/10.3390/ijms23169467.
Full textAbstract:
The fibroblast growth factor (FGF) family has various biological functions, including cell growth, tissue regeneration, embryonic development, metabolism, and angiogenesis. In the case of hair growth, several members of the FGF family, such as FGF1 and FGF2, are involved in hair growth, while FGF5 has the opposite effect. In this study, the regulation of the hair growth cycle by FGF12 was investigated. To observe its effect, the expression of FGF12 was downregulated in mice and outer root sheath (ORS) by siRNA transfection, while FGF12 overexpression was carried out using FGF12 adenovirus. For the results, FGF12 was primarily expressed in ORS cells with a high expression during the anagen phase of hair follicles. Knockdown of FGF12 delayed telogen-to-anagen transition in mice and decreased the hair length in vibrissae hair follicles. It also inhibited the proliferation and migration of ORS cells. On the contrary, FGF12 overexpression increased the migration of ORS cells. FGF12-overexpressed ORS cells induced the telogen-to-anagen transition in the animal model. In addition, FGF12 overexpression regulated the expression of PDGF-CC, MDK, and HB-EGF, and treatment of these factors exhibited hair growth promotion. Altogether, FGF12 promoted hair growth by inducing the anagen phase of hair follicles, suggesting the potential for hair loss therapy.
17
Kim, Sung Min, Jung-Il Kang, Hoon-Seok Yoon, et al. "HNG, A Humanin Analogue, Promotes Hair Growth by Inhibiting Anagen-to-Catagen Transition." International Journal of Molecular Sciences 21, no. 12 (2020): 4553. http://dx.doi.org/10.3390/ijms21124553.
Full textAbstract:
The hair follicle goes through repetitive cycles including anagen, catagen, and telogen. The interaction of dermal papilla cells (DPCs) and keratinocytes regulates the hair cycle and hair growth. Humanin was discovered in the surviving brain cells of patients with Alzheimer’s disease. HNG, a humanin analogue, activates cell growth, proliferation, and cell cycle progression, and it protects cells from apoptosis. This study was performed to investigate the promoting effect and action mechanisms of HNG on hair growth. HNG significantly increased DPC proliferation. HNG significantly increased hair shaft elongation in vibrissa hair follicle organ culture. In vivo experiment showed that HNG prolonged anagen duration and inhibited hair follicle cell apoptosis, indicating that HNG inhibited the transition from the anagen to catagen phase mice. Furthermore, HNG activated extracellular signal-regulated kinase (Erk)1/2, Akt, and signal transducer and activator of transcription (Stat3) within minutes and up-regulated vascular endothelial growth factor (VEGF) levels on DPCs. This means that HNG could induce the anagen phase longer by up-regulating VEGF, which is a Stat3 target gene and one of the anagen maintenance factors. HNG stimulated the anagen phase longer with VEGF up-regulation, and it prevented apoptosis by activating Erk1/2, Akt, and Stat3 signaling.
18
Kam, E., and M. B. Hodgins. "Communication compartments in hair follicles and their implication in differentiative control." Development 114, no. 2 (1992): 389–93. http://dx.doi.org/10.1242/dev.114.2.389.
Full textAbstract:
Observations on hair follicles presented in this paper show that boundaries to junctional communication are formed between groups of cells following different pathways of differentiation. The patterns of junctional communication in the bulbs of rat vibrissa follicles and human hair follicles were studied by microinjection of the fluorescent tracer dye Lucifer Yellow CH. Dye spread was extensive between undifferentiated cells of the hair bulb matrix but communication boundaries were found between groups of morphologically distinct cells. For example, boundaries to dye spread were observed between undifferentiated matrix cells and cells in the early stage of differentiation into the inner root sheath, between Huxley's and Henle's layers in the early inner root sheath and between cells of the cuticle and cortex of the hair. Dye did not spread between epithelial cells of the hair bulb and mesenchymal cells of the connective tissue sheath or dermal papilla. The patterns of dye spread became more complex (increased boundary formation and subcompartmentation) as differentiation progressed in higher regions of the hair bulb. The observed communication can be related to previous ultrastructural studies by others on the distribution of gap junctions in the wool follicle. These results show that junctional communication, with its consequent intercellular spread of small ions and molecules, is associated with uniformity of expression and behaviour within cell populations and that interruption of communication through the formation of boundaries and communication compartments is temporally and spatially related to the production of subpopulations of cells committed to the expression of different phenotypes.
19
Zhang, Shoubing, Huimin Hu, Huishan Zhang, et al. "Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo." Cell Transplantation 21, no. 6 (2012): 1075–85. http://dx.doi.org/10.3727/096368912x640538.
Full text
20
Jahoda, C. A., K. A. Horne, A. Mauger, S. Bard, and P. Sengel. "Cellular and extracellular involvement in the regeneration of the rat lower vibrissa follicle." Development 114, no. 4 (1992): 887–97. http://dx.doi.org/10.1242/dev.114.4.887.
Full textAbstract:
The sequence of events leading to the reconstruction of a fibre-producing hair follicle, after microsurgical amputation of the lower follicle bulb, has been detailed by immunohistology and electron microscopy. The initial response was essentially found to be a wound reaction, in that hyperproliferative follicle epidermis quickly spread to below the level of amputation—associated with downward movement of mesenchymal (or dermal) sheath cells. Fibronectin was prominent in both dermis and epidermis at this stage and, as in wound repair, preceded laminin and type IV collagen in covering the lower dermal-epidermal junction. Once a new basal line of epidermis and a complete basement membrane were established, laminin and type IV collagen were detected below this junction and within the prospective papilla-forming mesenchyme. This coincided with ultrastructural observations of profuse sub-basement membrane extracellular material in the region of new papilla formation. The glassy membrane displayed extensive ultrastructural modifications at its lower level, and these corresponded with localized variations in staining intensities for all three antibodies over time. The membrane hung below the level of the epidermis, and was crossed by migrating cells from the mesenchymal dermal sheath of the follicle - it acted to segregate the inner group of follicular dermal cells from wound fibroblasts. Extracellular matrix may be a mediator of the dermal-epidermal interactions associated with this hair follicle regeneration phenomenon.
21
Ku, Kyung-Eun, Nahyun Choi, and Jong-Hyuk Sung. "Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth." International Journal of Molecular Sciences 21, no. 16 (2020): 5672. http://dx.doi.org/10.3390/ijms21165672.
Full textAbstract:
Rab27a/b are known to play an important role in the transport of melanosomes, with their knockout causing silvery gray hair. However, the relationship between Rab27a/b and hair growth is not well known. To evaluate the role of Rab27a/b in hair cycle, we investigated the expression of Rab27a/b during hair cycling and human outer root sheath (hORS) cells. The expression of Rab27a in ORS cells was mainly detected at the anagen, whereas expression of Rab27b in ORS, and epidermal cells was strongly expressed at the telogen. Additionally, Rab27a/b were expressed in the Golgi of hORS cells. To evaluate the role of Rab27a/b in hair growth, telogen-to-anagen transition animal and vibrissae hair follicles (HFs) organ culture models were assayed using Rab27a/b siRNAs. The knockdown of Rab27a or Rab27b suppressed or promoted hair growth, respectively. These results were also confirmed in human dermal papilla cells (hDPCs) and hORS cells, showing the opposite mitogenic effects. Moreover, Rab27b knockdown increased the expression levels of various growth factors in the hDPCs and hORS cells. Overall, the opposite temporal expression patterns during hair cycling and roles for hair growth of Rab27a/b suggested that Rab27a/b might regulate the hair cycle. Therefore, our study may provide a novel solution for the development of hair loss treatment by regulating Rab27a/b levels.
22
Kang, Jung-Il, Hoon-Seok Yoon, Sung Kim, et al. "Mackerel-Derived Fermented Fish Oil Promotes Hair Growth by Anagen-Stimulating Pathways." International Journal of Molecular Sciences 19, no. 9 (2018): 2770. http://dx.doi.org/10.3390/ijms19092770.
Full textAbstract:
Hair growth is regulated by the interaction between dermal papilla cells (DPC) and other cells inside the hair follicle. Here, we show the effect and action mechanism of mackerel-derived fermented fish oil (FFO) extract and its component docosahexaenoic acid (DHA) in the control of hair growth. The hair growth effect of FFO extract was evaluated by the culture method of vibrissa follicles and in vivo dotmatrix planimetry method. FFO extract increased the length of hair-fibers and enabled stimulated initiation into the anagen phase of the hair cycle. As expected, FFO extract significantly increased DPC proliferation. FFO extract induced the progression of the cell cycle and the activation of extracellular signal-regulated kinase (ERK), p38 and Akt. FFO extract induced nuclear translocation of β-catenin, a stimulator of anagen phase, through an increase of phospho-glycogen synthase kinase3β (GSK3β) level. Since various prostaglandins are known to promote hair growth in humans and mice, we examined the effect of DHA, a main omega-3 fatty acid of FFO, on DPC proliferation. DHA not only increased DPC proliferation but also upregulated levels of cell cycle-associated proteins such as cyclin D1 and cdc2 p34. These results show that FFO extract and DHA promote hair growth through the anagen-activating pathways in DPC.
23
Li, Meiying, Jin Yu Liu, Shichao Wang, et al. "Multipotent Neural Crest Stem Cell-Like Cells from Rat Vibrissa Dermal Papilla Induce Neuronal Differentiation of PC12 Cells." BioMed Research International 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/186239.
Full textAbstract:
Bone marrow mesenchymal stem cells (BMSCs) transplants have been approved for treating central nervous system (CNS) injuries and diseases; however, their clinical applications are limited. Here, we model the therapeutic potential of dermal papilla cells (DPCs)in vitro. DPCs were isolated from rat vibrissae and characterized by immunocytofluorescence, RT-PCR, and multidifferentiation assays. We examined whether these cells could secrete neurotrophic factors (NTFs) by using cocultures of rat pheochromocytoma cells (PC12) with conditioned medium and ELISA assay. DPCs expressed Sox10, P75, Nestin, Sox9, and differentiated into adipocytes, osteoblasts, smooth muscle cells, and neurons under specific inducing conditions. The DPC-conditioned medium (DPC-CM) induced neuronal differentiation of PC12 cells and promoted neurite outgrowth. Results of ELISA assay showed that compared to BMSCs, DPCs secreted more brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). Moreover, we observed that, compared with the total DPC population, sphere-forming DPCs expressed higher levels of Nestin and P75 and secreted greater amounts of GDNF. The DPCs from craniofacial hair follicle papilla may be a new and promising source for treating CNS injuries and diseases.
24
LAVKER, ROBERT M., GEORGE COTSARELIS, ZHI-GANG WEI, and TUNG-TIEN SUN. "Stem Cells of Pelage, Vibrissae, and Eyelash Follicles: The Hair Cycle and Tumor Formationa." Annals of the New York Academy of Sciences 642, no. 1 (2006): 214–24. http://dx.doi.org/10.1111/j.1749-6632.1991.tb24389.x.
Full text
25
Filsell, W., J. C. Little, A. J. Stones, S. P. Granger, and S. A. Bayley. "Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype." Journal of Cell Science 107, no. 7 (1994): 1761–72. http://dx.doi.org/10.1242/jcs.107.7.1761.
Full textAbstract:
The dermal papilla is a discrete group of cells at the base of the hair follicle and is implicated in controlling the hair growth cycle. Early passage dermal papilla cells can induce hair growth in vivo, but, upon further culturing, this property is lost. In order to study the events occurring in hair induction, a representative dermal papilla cell line was required. We have transfected passage 1 rat vibrissa dermal papilla cells with a polyomavirus large T gene encoding a temperature-sensitive T antigen, and generated permanent cell lines in which the immortalizing function can be switched off by temperature shift. The cells established without crisis, resembled cells in the starting population, and retained the aggregative properties of early passage dermal papilla cells. Growth studies were performed on the immortalized cell lines, which showed that transferring the cells to the restrictive temperature for the large T gene product resulted in cell senescence or quiescence, and changes in morphology. Implantation of cell pellets into the ears of immunologically compatible rats showed that the immortal cells retained hair-inductive ability. Cytokines are believed to have an important role in the control of hair growth. The pattern of cytokine gene expression in the immortal cell lines was compared with early passage dermal papilla cells and a non-hair-inducing dermal papilla cell line, using reverse transcriptase-polymerase chain reaction. Epidermal growth factor, tumour necrosis factor, and interleukin-1a were detected in the immortalized and non-hair-inducing dermal papilla cell lines, but were absent in passage 2 dermal papilla cells. All other cytokines examined were detected in all the cell types under study. These results demonstrate that the polyomavirus large Ttsa-immortalized dermal papilla cell lines are very similar to passage 2 dermal papilla cells and thus provide a good model for hair growth studies. Cytokine expression profiles indicate that the expression of several cytokines may be implicated in hair induction. Further studies are under way to investigate the relationship between cytokine expression and the hair growth cycle.
26
Osada, Aki, Tokuro Iwabuchi, Jiro Kishimoto, Tatsuo S. Hamazaki, and Hitoshi Okochi. "Long-Term Culture of Mouse Vibrissal Dermal Papilla Cells and De Novo Hair Follicle Induction." Tissue Engineering 13, no. 5 (2007): 975–82. http://dx.doi.org/10.1089/ten.2006.0304.
Full text
27
Mii, Sumiyuki, Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman. "Comparison of Nestin-Expressing Multipotent Stem Cells in the Tongue Fungiform Papilla and Vibrissa Hair Follicle." Journal of Cellular Biochemistry 115, no. 6 (2014): 1070–76. http://dx.doi.org/10.1002/jcb.24696.
Full text
28
Yari, Abazar, Fatemeh Heidari, Sanaz Joulai Veijouye та Maliheh Nobakht. "Hair follicle stem cells promote cutaneous wound healing through the SDF-1α/CXCR4 axis: an animal model". Journal of Wound Care 29, № 9 (2020): 526–36. http://dx.doi.org/10.12968/jowc.2020.29.9.526.
Full textAbstract:
Objective: An appropriate source of adult stem cells for therapeutic use is stem cells deriving from the hair follicle bulge. Following injury, ischaemic tissues produce a variety of cytokines and growth factors that are essential for tissue repair. This study sought to investigate the temporal effects of hair follicle bulge stem cells (HFSCs) on cutaneous wound healing in rats using the SDF-1α/CXCR4 axis. Method: HFSCs obtained from rat vibrissa, labeled with DiI and then special markers, were detected using flow cytometry. The animals were divided into five groups: control (non-treated, n=18), sham (PBS, n=18), AMD (treated with AMD3100, n=18), HFSC + AMD (treated with HFSCs + AMD3100, n=18) and HFSC (treated with HFSCs, n=18). A full-thickness excisional wound model was created and DiI-labeled HFSCs were injected around the wound bed. Wound healing was recorded with digital photographs. The animals were sacrificed 3, 7 and 14 days after the surgery and were used for histological (H&E, Masson's trichrome staining) and molecular (ELISA and q-PCR) assays. Results: The flow cytometry results demonstrated that HFSCs were CD34-positive, nestin-positive, but Kr15-negative. The morphological analysis of the HFSC-treated wounds showed accelerated wound closure. The histological analysis of the photomicrographs exhibited more re-epithelialisation and dermal structural regeneration in the HFSC-treated wounds compared with the control group. In the HFSC + AMD group, the histological parameters improved on the same days, but showed a significant decrease compared with the HFSC group in all the days assayed. In the AMD group, there was a significant reduction in the noted parameters. qRT-PCR and ELISA showed a high expression level of SDF-1α, CXCR4 and VEGFR-2 in the HFSC-treated wounded skin tissue, but the expression of CXCR4 and VEGFR-2 showed a significant reduction in the HFSC + AMD group compared with the HFSC group. Conclusions: Based on the findings of this study, HFSC transplantation affects wound closure parameters and the expression of SDF-1α and CXCR4. As the SDF-1α expression level increases in the injured area, the HFSCs contribute to wound repair through the SDF-1α/CXCR4 axis. This result is extremely valuable because it raises the possibility of wounds healed by isolating autologous HFSCs from the patient.
29
Amoh, Yasuyuki, Sumiyuki Mii, Ryoichi Aki, et al. "Multipotent nestin-expressing stem cells capable of forming neurons are located in the upper, middle and lower part of the vibrissa hair follicle." Cell Cycle 11, no. 18 (2012): 3513–17. http://dx.doi.org/10.4161/cc.21803.
Full text
30
Kang, Jung-Il, Sang-Cheol Kim, Jae-Hee Hyun, et al. "Effect of Orostachys iwarenge Hara on the proliferation of dermal papilla ceIls." Journal of Medicine and Life Science 7, no. 1 (2010): 139–42. http://dx.doi.org/10.22730/jmls.2010.7.1.139.
Full textAbstract:
In this study, we investigated the hair-growth effect ofplants growing in Jeju by the proliferation of dermal papilla cells. Dermal papilla cells are specialized mesenchymal cells that located at the bulb region of hair follicles. The cells play crucial roles in hair formation, growth, and cycling. When immortalized vibrissa dermal papilla cells were treated with several extracts, the extract of Orostachys iwarenge Hara increased proliferation of immortalized vibrissa dermal papilla cells. These results suggest that O. iwarenge extract has the potential to promote hair growth via the proliferation of dermal papilla.
31
Horne, Kenneth A., Colin A. B. Jahoda, and Roy F. Oliver. "Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat." Development 97, no. 1 (1986): 111–24. http://dx.doi.org/10.1242/dev.97.1.111.
Full textAbstract:
Retention of the capacity to induce the growth of hair by cultured adult rat vibrissa dermal papilla cells has been investigated. Small pellets of serially cultured papilla cells were implanted into the bases of the exposed follicular epidermis of amputated adult rat vibrissa follicles. Amputated follicles that received no cell implants or implants of cultured dorsal skin fibroblasts were used as controls. Over 50% of follicles implanted with cultured papilla cells in the passage range 1–3 grew hairs. In contrast none of the follicles that received late passage cells (range 6–15) produced hairs; and spontaneous regeneration of hair occurred in only 3% of the control follicles. These results demonstrate that cultured papilla cells of early passage numbers retain their ability to induce hair growth. Histological examination confirmed that the implanted papilla cells interacted with follicular epidermis to organize the development of new, hair-producing bulbs, each containing a discrete dermal papilla. An important observation was that aggregative behaviour leading to papilla formation was only manifested by early passage papilla cell implants. This persisting embryonic characteristic appears to be an essential functional component of papilla cell activity which operates to regulate the profound morphogenetic changes that occur during the hair growth cycle.
32
Pisansarakit, P., and G. P. M. Moore. "Induction of hair follicles in mouse skin by rat vibrissa dermal papillae." Development 94, no. 1 (1986): 113–19. http://dx.doi.org/10.1242/dev.94.1.113.
Full textAbstract:
Rat vibrissa dermal papillae were transplanted between the epidermis and dermis of isolated embryonic mouse skin and then grafted onto nude mice. The papillae induced the formation of hair follicles which were larger than those of the host skin but smaller than vibrissa follicles. The potential of isolated dermal papillae to induce follicles with characteristics of those from which the papillae originated is discussed. One of the major factors affecting the sizes of induced follicles may have been related to the splitting of the papilla mass and dispersal of the cells by invading cords of epidermal cells from the host skin during induction.
33
Kang, Jung-Il, Youri Kim, Haeri Kim, Eun-Sook Yoo, and Hee-Kyoung Kang. "The Effect of Umbraulva japonica on the Stimulation of Anagen Phase." Yakhak Hoeji 67, no. 3 (2023): 182–88. http://dx.doi.org/10.17480/psk.2023.67.3.182.
Full textAbstract:
This study was conducted to evaluate the effects of Umbraulva japonica extract on the hair growth. Treatment with U. Japonica extract significantly increased the proliferation of dermal papilla cells (DPCs), a central regulator of the hair cycle. When isolated rat vibrissa follicles were treated with U. Japonica extract for 21 d, the hair-fiber lengths of the vibrissa follicles increased. When examined the activity of 5α-reductase, which converts testosterone to dihydrotestosterone (DHT), a main cause of androgenetic alopecia, U. Japonica extract did not inhibit the 5α-reductase activity. Moreover, we found that the U. Japonica extract also significantly accelerated anagen phase in vivo. These results suggest that the U. Japonica extract has the potential to treat alopecia via the stimulation of anagen phase.
34
Kang, Jung-Il, Youn Kyung Choi, Sang-Chul Han та ін. "5-Bromo-3,4-dihydroxybenzaldehyde Promotes Hair Growth through Activation of Wnt/β-Catenin and Autophagy Pathways and Inhibition of TGF-β Pathways in Dermal Papilla Cells". Molecules 27, № 7 (2022): 2176. http://dx.doi.org/10.3390/molecules27072176.
Full textAbstract:
Various studies addressing the increasing problem of hair loss, using natural products with few side effects, have been conducted. 5-bromo-3,4-dihydroxybenzaldehyde (BDB) exhibited anti-inflammatory effects in mouse models of atopic dermatitis and inhibited UVB-induced oxidative stress in keratinocytes. Here, we investigated its stimulating effect and the underlying mechanism of action on hair growth using rat vibrissa follicles and dermal papilla cells (DPCs), required for the regulation of hair cycle and length. BDB increased the length of hair fibers in rat vibrissa follicles and the proliferation of DPCs, along with causing changes in the levels of cell cycle-related proteins. We investigated whether BDB could trigger anagen-activating signaling pathways, such as the Wnt/β-catenin pathway and autophagy in DPCs. BDB induces activation of the Wnt/β-catenin pathway through the phosphorylation of GSG3β and β-catenin. BDB increased the levels of autophagic vacuoles and autophagy regulatory proteins Atg7, Atg5, Atg16L, and LC3B. We also investigated whether BDB inhibits the TGF-β pathway, which promotes transition to the catagen phase. BDB inhibited the phosphorylation of Smad2 induced by TGF-β1. Thus, BDB can promote hair growth by modulating anagen signaling by activating Wnt/β-catenin and autophagy pathways and inhibiting the TGF-β pathway in DPCs.
35
Renehan, W. E., M. F. Jacquin, R. D. Mooney, and R. W. Rhoades. "Structure-function relationships in rat medullary and cervical dorsal horns. II. Medullary dorsal horn cells." Journal of Neurophysiology 55, no. 6 (1986): 1187–201. http://dx.doi.org/10.1152/jn.1986.55.6.1187.
Full textAbstract:
In Nembutal-anesthetized rats, 31 physiologically identified medullary dorsal horn (MDH) cells were labeled with horseradish peroxidase (HRP). Ten responded only to deflection of one or more vibrissae. Six cells were activated by guard hair movement only, six by deflection of guard hairs or vibrissa(e), and seven by pinch of facial skin with serrated forceps. Different classes of low-threshold cells could not be distinguished on the basis of their somadendritic morphologies or laminar distribution. Neurons activated by multiple vibrissae were unique, however, in that one sent its axon into the medial lemniscus, and three projected into the trigeminal spinal tract. None of the guard hair-only or vibrissae-plus-guard hair neurons had such projections. Cells that responded best to noxious stimulation were located mainly in laminae I, II, and deep V, while neurons activated by vibrissa(e) and/or guard hair deflection were located in layers III, IV, and superficial V. Low-threshold neurons generally had fairly thick dendrites with few spines, whereas high-threshold cells tended to have thinner dendrites with numerous spines. Moreover, the dendritic arbors of low-threshold cells were, for the most part, denser than those of the noxious cells. Neurons with mandibular receptive fields were located in the dorsomedial portion of the MDH; cells with ophthalmic fields were found in the ventrolateral MDH, and maxillary cells were interposed. Cells sensitive to deflection of dorsal mystacial vibrissae and/or guard hairs were located ventral to those activated by more ventral hairs. Neurons with rostral receptive fields were found in the rostral MDH, while cells activated by hairs of the caudal mystacial pad, periauricular, and periorbital regions were located in the caudal MDH. Receptive-field types were encountered that have not been reported for trigeminal primary afferent neurons: multiple vibrissae; vibrissae plus guard hairs; and wide dynamic range. The latter two can be explained by the convergence of different primary afferent types onto individual neurons. Our failure to find a significant relationship between dendritic area (in the transverse plane) and the number of vibrissae suggests that primary afferent convergence may not be responsible for the synthesis of the multiple vibrissae receptive field. Excitatory connections between MDH neurons may, therefore, account for multiple vibrissae receptive fields in the MDH.
36
Nussbaumer, J. C., and H. Van der Loos. "An electrophysiological and anatomical study of projections to the mouse cortical barrelfield and its surroundings." Journal of Neurophysiology 53, no. 3 (1985): 686–98. http://dx.doi.org/10.1152/jn.1985.53.3.686.
Full textAbstract:
This study establishes a cortical map of the somatosensory periphery of the mouse head, with emphasis on the whisker pad. Data in the literature on the projection of the common hair follicles are confusing, notably the question whether or not this projection is separated from or overlaps with that of the facial vibrissae, the barrelfield. Microelectrode recordings in the barrelfield and its immediate surroundings upon natural stimulation of the periphery were followed by microlesions and histological reconstruction. Results show that the barrelfield consists of two parts: an anterior part where vibrissal follicles and the skin bearing them are represented, and a posterior part receiving only vibrissa inputs. The skin between these latter vibrissae is represented outside the barrelfield. We conclude that the partially dissociated cortical representation of skin and vibrissae may allow large vibrissae to be used in tasks requiring greater acuity than the shorter ones provide. This hypothesis is supported by the observation that, owing to special muscles, large vibrissae are more mobile than short ones. We also propose that the segregation of inputs from vibrissae and common fur is related to the number of nerve fibers serving one follicle, and we indicate an experimental model to test this possibility.
37
Kumamoto, Tadashi, David Shalhevet, Hiroyuki Matsue, et al. "Hair follicles serve as local reservoirs of skin mast cell precursors." Blood 102, no. 5 (2003): 1654–60. http://dx.doi.org/10.1182/blood-2003-02-0449.
Full textAbstract:
AbstractSeveral leukocyte populations normally reside in mouse skin, including Langerhans cells and γδ T cells in the epidermis and macrophage and mast cells in the dermis. Interestingly, these skin resident leukocytes are frequently identified within or around hair follicles (HFs), which are known to contain stem cell populations that can generate the epidermal architecture or give rise to the melanocyte lineage. Thus, we reasoned that HFs might serve as a local reservoir of the resident leukocyte populations in the skin. When vibrissal follicles of adult mice were cultured in the presence of stem cell factor (SCF), interleukin 3 (IL-3), IL-7, granulocyte-macrophage colony-stimulating factor, and Flt3 ligand, CD45+/lineage–/c-kit+/FcϵRI+ cells became detectable on the outgrowing fibroblasts in 10 days and expanded progressively thereafter. These HF-derived leukocytes showed characteristic features of connective tissue-type mast cells, including proliferative responsiveness to SCF, metachromatic granules, mRNA expression for mast cell proteases-1, -4, -5, and -6, and histamine release on ligation of surface IgE or stimulation with substance P or compound 48/80. These results, together with our findings that HFs contain c-kit+ cells and produce SCF mRNA and protein, suggest that HFs provide a unique microenvironment for local development of mast cells.
38
Sarko, Diana K., Frank L. Rice, and Roger L. Reep. "Elaboration and Innervation of the Vibrissal System in the Rock Hyrax (Procavia capensis)." Brain, Behavior and Evolution 85, no. 3 (2015): 170–88. http://dx.doi.org/10.1159/000381415.
Full textAbstract:
Mammalian tactile hairs are commonly found on specific, restricted regions of the body, but Florida manatees represent a unique exception, exhibiting follicle-sinus complexes (FSCs, also known as vibrissae or tactile hairs) on their entire body. The orders Sirenia (including manatees and dugongs) and Hyracoidea (hyraxes) are thought to have diverged approximately 60 million years ago, yet hyraxes are among the closest relatives to sirenians. We investigated the possibility that hyraxes, like manatees, are tactile specialists with vibrissae that cover the entire postfacial body. Previous studies suggested that rock hyraxes possess postfacial vibrissae in addition to pelage hair, but this observation was not verified through histological examination. Using a detailed immunohistochemical analysis, we characterized the gross morphology, innervation and mechanoreceptors present in FSCs sampled from facial and postfacial vibrissae body regions to determine that the long postfacial hairs on the hyrax body are in fact true vibrissae. The types and relative densities of mechanoreceptors associated with each FSC also appeared to be relatively consistent between facial and postfacial FSCs. The presence of vibrissae covering the hyrax body presumably facilitates navigation in the dark caves and rocky crevices of the hyrax's environment where visual cues are limited, and may alert the animal to predatory or conspecific threats approaching the body. Furthermore, the presence of vibrissae on the postfacial body in both manatees and hyraxes indicates that this distribution may represent the ancestral condition for the supraorder Paenungulata.
39
Stenn, Kurt S., Leonardo A. Fernandez, and Shirley J. Tirrell. "The Angiogenic Properties of the Rat Vibrissa Hair Follicle Associate with the Bulb." Journal of Investigative Dermatology 90, no. 3 (1988): 409–11. http://dx.doi.org/10.1111/1523-1747.ep12456517.
Full text
40
Waters, James M., Gavin D. Richardson, and Colin A. B. Jahoda. "Hair follicle stem cells." Seminars in Cell & Developmental Biology 18, no. 2 (2007): 245–54. http://dx.doi.org/10.1016/j.semcdb.2007.02.003.
Full text
41
Vermorken, Alphons J. M., Hans Verhagen, C. (Elly) M. A. A. Goos, Marie-Cécile Lenoir, and Braham Shroot. "Psoriatic hair-follicle cells." Differentiation 29, no. 2 (1985): 182–88. http://dx.doi.org/10.1111/j.1432-0436.1985.tb00313.x.
Full text
42
Lavker, Robert M., Tung-Tien Sun, Hideo Oshima, et al. "Hair Follicle Stem Cells." Journal of Investigative Dermatology Symposium Proceedings 8, no. 1 (2003): 28–38. http://dx.doi.org/10.1046/j.1523-1747.2003.12169.x.
Full text
43
Lenoir, M. C., E. Vromans, B. Shroot, and A. J. M. Vermorken. "Proriatic hair follicle cells." Molecular Biology Reports 11, no. 4 (1986): 247–52. http://dx.doi.org/10.1007/bf00419604.
Full text
44
Kim, Jung Hyun, Tae Yoon Kim, Bonhyuk Goo, and Yeoncheol Park. "Bee Venom Stimulates Growth Factor Release from Adipose-Derived Stem Cells to Promote Hair Growth." Toxins 16, no. 2 (2024): 84. http://dx.doi.org/10.3390/toxins16020084.
Full textAbstract:
Limited evidence suggests that stimulating adipose-derived stem cells (ASCs) indirectly promotes hair growth. We examined whether bee venom (BV) activated ASCs and whether BV-induced hair growth was facilitated by enhanced growth factor release by ASCs. The induction of the telogen-to-anagen phase was studied in mice. The underlying mechanism was investigated using organ cultures of mouse vibrissa hair follicles. When BV-treated ASCs were injected subcutaneously into mice, the telogen-to-anagen transition was accelerated and, by day 14, the hair weight increased. Quantitative polymerase chain reaction (qPCR) revealed that BV influenced the expression of several molecules, including growth factors, chemokines, channels, transcription factors, and enzymes. Western blot analysis was employed to verify the protein expression levels of extracellular-signal-regulated kinase (ERK) and phospho-ERK. Both the Boyden chamber experiment and scratch assay confirmed the upregulation of cell migration by BV. Additionally, ASCs secreted higher levels of growth factors after exposure to BV. Following BV therapy, the gene expression levels of alkaline phosphatase (ALP), fibroblast growth factor (FGF)-1 and 6, endothelial cell growth factor, and platelet-derived growth factor (PDGF)-C were upregulated. The findings of this study suggest that bee venom can potentially be utilized as an ASC-preconditioning agent for hair regeneration.
45
Panteleyev, Andrei A., Colin A. B. Jahoda, and Angela M. Christiano. "Hair follicle predetermination." Journal of Cell Science 114, no. 19 (2001): 3419–31. http://dx.doi.org/10.1242/jcs.114.19.3419.
Full textAbstract:
Recent genetic and molecular studies of hair follicle (HF) biology have provided substantial insight; however, the molecular data, including expression patterns, cannot be properly appreciated without an understanding of the basic cellular rearrangements and interactions that underpin HF cyclic transformations. We present a novel interpretation of the major cellular processes that take place during HF cycling – the hypothesis of hair follicle predetermination. This hypothesis is an extension of previous models of HF cellular kinetics but has two critical modifications: the dual origin of the cycling portion of the HF, and the timing of the recruitment of stem cells. A compilation of evidence suggests that the ascending portion of the HF (hair shaft and inner root sheath) arises not from bulge-located HF stem cells that contribute to the formation of only the outer root sheath (ORS), but instead from the germinative cells localized in the secondary hair germ. In middle anagen, upon completion of the downward growth of the HF, cells derived from the bulge region migrate downward along the ORS to reside at the periphery of the HF bulb as a distinct, inactive cell population that has specific patterns of gene expression - ‘the lateral disc’. These cells survive catagen-associated apoptosis and, under the direct influence of the follicular papilla (FP), transform into the hair germ and acquire the ability to respond to FP signaling and produce a new hair. Thus, we propose that the specific sensitivity of germ cells to FP signaling and their commitment to produce the ascending HF layers are predetermined by the previous hair cycle during the process of transformation of bulge-derived lateral disc cells into the secondary hair germ.
46
Ma, DR, EN Yang, and ST Lee. "A Review: The Location, Molecular Characterisation and Multipotency of Hair Follicle Epidermal Stem Cells." Annals of the Academy of Medicine, Singapore 33, no. 6 (2004): 784–88. http://dx.doi.org/10.47102/annals-acadmedsg.stlee.
Full textAbstract:
Introduction: Recent work has focused on the hair follicle as the main repository of multipotent stem cells in skin, which is a neat model to study the mechanisms regulating the proliferation, migration and final fate of adult stem cells. This review examines the available literature for its location, molecular markers and multipotency. Methods: Peer-reviewed journals and monographs on the subject were covered. Results: With the application of stem cell-labelling techniques and clonogenicity assay, it is clear that most of the hair follicle stem cells are located at the bulge region, but the base of the hair follicle does contain some clonogenic cells; whether they are stem cells is still unknown. Extensive works have been done in identifying hair follicle stem cells. The potential markers for hair follicle stem cells include: b1-integrin, keratin 19, a6-integrin, CD71, p63, and CD34. Most of these markers are expressed in high levels in hair follicle stem cells, but there is still difficulty in distinguishing hair follicle stem cells from their transit-amplifying progeny, and the sorted hair follicle stem cells with these markers are far from pure. As hair follicle stem cells might have been activated after leaving the stem cell niche, the markers for cells in vitro might not be identical to those in vivo. Using double-labelling techniques with BrdU and 3H-Thymidine, and the creation of novel chimera transgenic mice, it was proved that hair follicle stem cells can repopulate wound epidermis, forming epidermis, hair follicles and sebaceous glands, but it contributes little to the epidermis in physiological condition, except the hair follicle. Conclusions: Slow cycling, label-retaining cells exist at the bulge of the hair follicle, with high proliferative potential and clonogenicity. The putative bulge stem cells can contribute to the epidermis, outer root sheath, inner root sheath, hair shaft and sebaceous gland. However, they still lack certain markers to distinguish bulge stem cells from their progeny, and much work needs to focus on the interrelations between bulge cells and interfollicular keratinocyte stem cells, the relations between bulge cells and dermal papilla mesenchyme cells, and the mechanism of hair growth.
47
Hamida, Ons Ben, Moon Kyu Kim, Young Kwan Sung, Min Kyu Kim, and Mi Hee Kwack. "Hair Regeneration Methods Using Cells Derived from Human Hair Follicles and Challenges to Overcome." Cells 14, no. 1 (2024): 7. https://doi.org/10.3390/cells14010007.
Full textAbstract:
The hair follicle is a complex of mesenchymal and epithelial cells acquiring different properties and characteristics responsible for fulfilling its inductive and regenerative role. The epidermal and dermal crosstalk induces morphogenesis and maintains hair follicle cycling properties. The hair follicle is enriched with pluripotent stem cells, where dermal papilla (DP) cells and dermal sheath (DS) cells constitute the dermal compartment and the epithelial stem cells existing in the bulge region exert their regenerative role by mediating the epithelial–mesenchymal interaction (EMI). Many studies have developed and focused on various methods to optimize the EMI through in vivo and in vitro approaches for hair regeneration. The culturing of human hair mesenchymal cells resulted in the loss of trichogenicity and inductive properties of DP cells, limiting their potential application in de novo hair follicle generation in vivo. Epithelial stem cells derived from human hair follicles are challenging to isolate and culture, making it difficult to obtain enough cells for hair regeneration purposes. Mesenchymal stem cells and epithelial stem cells derived from human hair follicles lose their ability to form hair follicles during culture, limiting the study of hair follicle formation in vivo. Therefore, many attempts and methods have been developed to overcome these limitations. Here, we review the possible and necessary cell methods and techniques used for human hair follicle regeneration and the restoration of hair follicle cell inductivity in culture.
48
Suwarna, Nancy, Veronika Maria Sidharta, Lorettha Wijaya, and Sukmawati Tansil Tan. "Unlocking the potential of the secretome in hair growth enhancement." International Journal of Basic & Clinical Pharmacology 13, no. 5 (2024): 732–38. http://dx.doi.org/10.18203/2319-2003.ijbcp20242439.
Full textAbstract:
Hair growth and its regulation are critical in biomedical research due to the widespread issue of hair loss. Understanding the cellular and molecular mechanisms of hair follicle development, cycling, and regeneration is essential for identifying therapeutic targets for hair growth disorders. This comprehensive literature review examined research on the biological pathways of hair follicle development, the role of the secretome in hair regeneration, and the efficacy of current hair loss treatments. Databases such as PubMed, Scopus, and Web of Science were meticulously searched for relevant studies, with a particular focus on the effects of the secretome on hair growth. The review highlights the significant role of hair follicle stem cells in wound healing through re-epithelialization and neogenesis, influenced by inflammatory cells. Exosomes from hair follicle mesenchymal stem cells containing lncRNA H19 enhanced diabetic wound healing by promoting cell proliferation and inhibiting pyroptosis. Hair follicle transplantation demonstrated the activation and differentiation of stem cells, with molecular signaling pathways between epithelial and mesenchymal cells being crucial for hair follicle regeneration. PlncRNA-1 promoted hair follicle stem cell differentiation via the EZH2/ZEB1/MAPK1 axis, enhancing wound healing. Secretome-based therapies offer a promising multifaceted approach to stimulating hair follicles and promoting hair regeneration, potentially overcoming the limitations and side effects of current treatments like minoxidil and finasteride. Understanding these mechanisms provides valuable insights for future therapeutic development in hair growth disorders.
49
Mattson, Erin E., and Christopher D. Marshall. "Follicle Microstructure and Innervation Vary between Pinniped Micro- and Macrovibrissae." Brain, Behavior and Evolution 88, no. 1 (2016): 43–58. http://dx.doi.org/10.1159/000447551.
Full textAbstract:
Histological data from terrestrial, semiaquatic, and fully aquatic mammal vibrissa (whisker) studies indicate that follicle microstructure and innervation vary across the mystacial vibrissal array (i.e. medial microvibrissae to lateral macrovibrissae). However, comparative data are lacking, and current histological studies on pinniped vibrissae only focus on the largest ventrolateral vibrissae. Consequently, we investigated the microstructure, medial-to-lateral innervation, and morphometric trends in harp seal (Pagophilus groenlandicus) vibrissal follicle-sinus complexes (F-SCs). The F-SCs were sectioned either longitudinally or in cross-section and stained with a modified Masson's trichrome stain (microstructure) or Bodian's silver stain (innervation). All F-SCs exhibited a tripartite blood organization system. The dermal capsule thickness, the distribution of major branches of the deep vibrissal nerve, and the hair shaft design were more symmetrical in medial F-SCs, but these features became more asymmetrical as the F-SCs became more lateral. Overall, the mean axon count was 1,221 ± 422.3 axons/F-SC and mean axon counts by column ranged from 550 ± 97.4 axons/F-SC (medially, column 11) to 1,632 ± 173.2 axons/F-SC (laterally, column 2). These values indicate a total of 117,216 axons innervating the entire mystacial vibrissal array. The mean axon count of lateral F-SCs was 1,533 ± 192.9 axons/ F-SC, which is similar to values reported in the literature for other pinniped F-SCs. Our data suggest that conventional studies that only examine the largest ventrolateral vibrissae may overestimate the total innervation by ∼20%. However, our study also accounts for variation in quantification methods and shows that conventional analyses likely only overestimate innervation by ∼10%. The relationship between axon count and cross-sectional F-SC surface area was nonlinear, and axon densities were consistent across the snout. Our data indicate that harp seals exhibit microstructural and innervational differences between their microvibrissae (columns 8-11) and macrovibrissae (columns 1-7). We hypothesize that this feature is conserved among pinnipeds and may result in functional compartmentalization within their mystacial vibrissal arrays.
50
Yang, Leilei, and Ruiyun Peng. "Unveiling Hair Follicle Stem Cells." Stem Cell Reviews and Reports 6, no. 4 (2010): 658–64. http://dx.doi.org/10.1007/s12015-010-9172-z.
Full text