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1
Kulkarni, A. A. "Role of enzymes/inhibitors from vigna radiata in host-pathogen interactions." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2007. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2539.
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Smith, Kristina J. "In vitro biosynthesis of pectic polysaccharides." Thesis, University of Stirling, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322091.
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Bordenave, Marianne. "Les pectine esterases de l'hypocotyle de vigna radiata : nature, fonctionnement et roles." Paris 6, 1995. http://www.theses.fr/1995PA066265.
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Des pectine methylesterases (pme, e. C. 3. 1. 1. 11), enzymes participant au controle du degre de methylation des pectines, donc de la cohesion de l'apoplasme, ont ete extraites de parois d'hypocotyles de vigna radiata. L'eluat salin des parois isolees a ete fractionne sur colonne de carboxy-methyl sepharose ; les pics d'activite esterase obtenus contenaient au moins trois isoformes de pme, ainsi qu'une acetyle-esterase (ae). Ces differentes activites ont ensuite ete purifiees par fplc. Les trois pme purifiees, pe#, pe# et pe#, presentaient respectivement des pi de 7,5, 9,3, et 9,8, des mr de 45 000, 34 000 et 35 000 daltons, et des ph optimums de 6,0, 7,6 et 5,6. Une quatrieme activite pme, pe#, de pi acide, a ete detectee dans le fluide extracellulaire. L'ae, active sur les pectines acetylees non methylees, presentait un pi>9, une mr de 43 300, et un ph optimum de 6,5. Des sequences proteiques internes et n-terminales ont ete realisees a partir des 3 pme purifiees et de l'ae. Les sequences obtenues ont permis de cloner par pcr une partie du gene de l'ae, ainsi qu'une partie importante du gene de pe#. Le comportement enzymologique de pe#, pe#, et pe# a ete etudie en fonction du ph et de la concentration en sels. Des experiences d'elutions sequentielles des parois isolees de l'hypocotyle semblent indiquer que seule pe# est active in situ. Enfin, les activites pme et ae ont ete suivies le long de l'hypocotyle. Les parties hautes de l'hypocotyle sont enrichies en pe#, et les parties basses en pe#, pe#, pe#, et en ae. Le clonage de chacune des activites purifiees doit etre envisage pour permettre une meilleure comprehension de leurs roles respectifs au sein de la paroi
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Ahmed, Selina. "Studies on photosynthetic damage by waterlogging in mungbean (Vigna radiata (L.) Wilczak)." Kyoto University, 2002. http://hdl.handle.net/2433/149516.
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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第9800号<br>農博第1295号<br>新制||農||854(附属図書館)<br>学位論文||H14||N3722(農学部図書室)<br>UT51-2003-B340<br>京都大学大学院農学研究科地域環境科学専攻<br>(主査)教授 櫻谷 哲夫, 教授 堀江 武, 教授 泉井 桂<br>学位規則第4条第1項該当
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Parker, Beverly Jean 1944. "Water movement, structure and physiology in mung bean (Vigna radiata L.) leaves." Thesis, The University of Arizona, 1992. http://hdl.handle.net/10150/278118.
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Eight-day-old mung bean seedlings (Vigna radiata L.) grown in hydropondic medium were osmotically stressed by exposing roots to increasing concentrations of NaCl up to 4 bars. They were transferred after 16 to 18 hours to a similar solution containing tritiated water (THO). Periodic samples were taken of water transpiring from the leaves and of tissue water obtained from the same leaves, frozen and ground; specific radioactivity was determined by a scintillation counter. Proportional to increasing stress, the labelling of tissue water was increasingly delayed, the time for equilibration of the specific radioactivity in the two fractions lengthened, and equilibration occurred at higher concentrations of THO. Thus stress causes transpirational water to be increasingly restricted to extra-cellular pathways. Further investigations of stomatal function by leaf surface, of anatomy and of growth patterns were unsuccessful in finding an explanation for this behavior but did reveal a transpirational circadian rhythm and a continual layer of (air?) space between the palisade and spongy mesophyll, the latter organized into two compact rows.
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CHABANET, AGNES. "Peroxydases et laccase des parois de l'hypocotyle de vigna radiata : caracterisation et localisation." Paris 6, 1993. http://www.theses.fr/1993PA066331.
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Les peroxydases parietales peuvent participer a la rigidification des parois en catalysant la formation de ponts diphenols. Elles peuvent egalement intervenir, conjointement a des polyphenoloxydases (laccases), dans les parois en cours de lignification lors de la polymerisation des monolignols. Nous avons cherche a caracteriser et a localiser ces enzymes dans un materiel, l'hypocotyle de vigna radiata, qui presente un gradient de croissance bien defini. Apres avoir isole les proteines liees ioniquement aux parois, nous avons separe trois groupes de peroxydases cationiques egalement capables d'utiliser, mais de facon tres faible, l'oxygene. La peroxydase la plus cationique s'est revelee etre un trimere constitue par l'association de trois polypeptides. De plus, cette isoperoxydase etait celle qui presentait l'activite oxydase la plus significative. Une polyphenoloxydase (ppo), incapable d'oxyder le gaiacol ou les sels de l'acide ferulique, a egalement ete isolee. Il s'agit d'un polypeptide de 43 kd, dont l'activite est inhibee par le ctab mais non par les sels de l'acide ferulique et qui est reconnue par un immunserum anti-laccase obtenu a partir de laccases de cellules d'acer pseudoplatanus. Il s'agit donc tres probablement d'une laccase. Cette laccase a ete detectee uniquement dans les parois secondaires du xyleme qui contiennent egalement deux peroxydases, ce qui renforce l'hypothese d'une cooperation de ces deux enzymes dans la biosynthese de la lignine. En revanche, contrairement aux peroxydases, elle n'a jamais ete observee dans les parois ni des cellules epidermiques ni du parenchyme cortical, ce qui exclut une intervention des laccases dans la reticulation des parois primaires, tout au moins lors de l'arret de la croissance en longueur de l'organe
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Noble, Thomas J. "Molecular characterisation and identification of Pseudomonas savastanoi pv. Phaseolicola, infecting mungbeans in Australia." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/205533/1/Thomas_Noble_Thesis.pdf.
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This research explores population genetics for the bacterial pathogen Pseudomonas savastanoi pv. phaseolicola, which causes halo blight disease in its host mungbeans (Vigna radiata). The pathogen and host populations were investigated at a board scale using field and glasshouse studies and in detail using molecular biology techniques including qPCR and next-generation sequencing. The study found both the bacterial pathogen and host (mungbean) to have highly conserved genetic backgrounds. This will make it easier for breeders to target critical resistance genes to prevent the infection of the halo blight pathogen in future cultivars.
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Amaral, Ana Lúcia da Silva. "Isolamento da vicilina do feijão mungo verde (Vigna radiata L.) e estudo de suas atividades hipocolesterolêmica e antimicrobiana /." Araraquara, 2016. http://hdl.handle.net/11449/144669.
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Orientador: Aureluce Demonte<br>Banca: Rosiane Gomes Silva Oliveira<br>Banca: Thais Milena de Souza Bezerra<br>Banca: Tais Maia Bauab<br>Banca: Juliana Cristina Bassan<br>Resumo: A homologia entre as características estruturais, sequenciais e funcionais da β- conglicinina da soja em relação a outras vicilinas de leguminosas e a ação de seus peptídeos bioativos nos estimulou a investigar a atividade da globulina vicilina (8S) do feijão mungo verde como composto funcional. Objetivo: isolar e caracterizar a globulina 8S do feijão mungo verde e verificar a ação hipocolesterolêmica e antimicrobiana de seus hidrolisados em testes in vitro. Método: a proteína majoritária 8S do feijão mungo verde foi extraída a partir do método de isolamento, em seguida foi parcialmente purificada por cromatografia de permeação molecular, determinada sua massa molecular a partir da eluição em Coluna Sephadex G-200 e suas subunidades foram caracterizadas por SDS Page. A proteína purificada foi submetida à hidrólise enzimática sequencial com pepsina-pancreatina e o hidrolisado obtido caracterizado por permeação molecular e SDS Tricina. Diferentes frações provenientes da eluição do hidrolisado em Sephadex G-25 foram testadas quanto à inibição da HMG CoAr (3 - Hidróxi - 3 - metilglutaril - CoA redutase) e de microrganismos (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 e Helicobacter pylori ATCC 43504). Resultados: A 8S do feijão mungo verde é composta por polipeptídeos de 26, 29, 48 e 61 kDa e sua massa molecular é de 158,23±10 kDa, características condizentes com as vicilinas de outras leguminosas. As frações obtidas da eluição do hidrolisado em Sepahadex G-25... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The homology between the structural and functional characteristics of sequential β- conglycinin soy compared to other legume vicilin and the action of their bioactive peptides stimulated us to investigate the activity of globulin vicilin (8S) mung beans as functional compound. Objective: isolate and characterize the 8S globulin mung beans and check the hypocholesterolemic and antimicrobial activities their hydrolysates in in vitro tests. Method: The major protein 8S mung bean is extracted from the isolation method, then was partially purified by molecular permeation chromatography, its molecular weight determined from the elution Sephadex G- 200 and its subunits were characterized by SDSPAGE. The purified protein was subjected to sequential enzymatic hydrolysis with pepsin-pancreatin and the hydrolyzate characterized by molecular permeation and Tricine SDS. Different fractions from the hydrolyzate eluted on Sephadex G-25 were tested for inhibition of HMG CoAr (3 - hydroxy - 3 - methylglutaryl - CoA reductase) and microorganisms (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Helicobacter pylori ATCC 43504). Results: 8S mung bean is comprised of polypeptides of 26, 29, 48 and 61 kDa and its molecular weight is 158.23 ± 10 kDa, consistent with the characteristics vicilins from other legumes. The fractions obtained from the elution hydrolyzate in Sepahadex G-25 (10, 12, 14, 22 and 32) demonstrated significant in vitro inhibition of HMG-CoAr enzyme activity compared with the recognized hypocholesterolemic action drug (pravastatin), suggesting involvement of these peptide fractions in inhibiting an important step in cholesterol synthesis. The hydrolyzate obtained from sequential hydrolysis with pepsin-pancreatin presented antimicrobial activity against the tested microorganisms, particularly against H. pylori, is a promising advance in... (Complete abstract click electronic access below)<br>Doutor
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Nogueira, Alexandre Verzani. "Manganese toxicity in mungbean [Vigna radiata (L.) Wilczek] and the effects of vesicular-arbuscular mycorrhiza on plant manganese tolerance." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384870.
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Allison, Patricia J. "Biosynthesis of pectic 1,4 #beta#-D-galactan in mung bean (Vigna radiata) and related studies on tomato (Lycopersicon esculentum)." Thesis, University of Stirling, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322046.
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Caldas, Marília Tiberi. "Qualidade fisiológica de sementes e brotos de feijão-mungo- verde." Universidade Federal de Viçosa, 2004. http://www.locus.ufv.br/handle/123456789/10564.
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Previous issue date: 2004-03-11<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Os objetivos do presente trabalho foram avaliar a qualidade fisiológica das sementes de feijão-mungo-verde; a influência do etileno e do ácido giberélico (AG3) no crescimento dos brotos; a relação entre a atividade da enzima peroxidase e o escurecimento dos brotos; e determinar o teor de água das sementes e dos brotos. Para analisar a qualidade fisiológica das sementes foram utilizados os testes de germinação, envelhecimento acelerado (42 °C/48 h e 42 °C/72 h), germinação a baixa temperatura (18 °C) e condutividade elétrica. A determinação da atividade da peroxidase foi realizada em raiz, hipocótilo e folha verdadeira dos brotos, em diferentes pHs (3, 4, 5, 6, 7, 8 e 9), provenientes de sementes germinadas a 25 °C e a 30 °C, em dois estádios de desenvolvimento (brotos com três e seis dias), enquanto a inativação da enzima, realizada por tratamento térmico (90 °C), em diferentes períodos (0; 2,5; 5 e 10 min). O etileno (Ethrel) e o ácido giberélico (AG3) foram aplicados isoladamente ou em conjunto, em diferentes estádios da germinação das sementes. O teor de água destas e dos brotos foi determinado em estufa a 70 °C até peso constante. As linhagens testadas foram Ouro Verde MG 2, VC 1973A, VC 5734A, V 3476, VC 4039A, VC 6148B16, VC 3890B e VC 3902A do banco de germoplasma do EPAMIG/CTZM, mas, para avaliação enzimática e aplicação dos hormônios de crescimento, utilizou-se apenas a cultivar Ouro Verde MG 2. Os testes de envelhecimento acelerado e de condutividade elétrica não foram eficientes na avaliação do vigor das sementes, sendo o mais adequado o teste de germinação à baixa temperatura. A atividade da peroxidase foi expressa por uma curva de formato semelhante nas diferentes partes amostradas do broto, sendo mais baixa nos extremos de pHs 3 ou 9 e mais elevada entre os pHs 4 e 6. Os picos máximos de atividade da enzima foram observados nos pHs 4 e 5 em raiz e 5 e 6 em folha e hipocótilo. Esse formato indica que existem diferentes isoenzimas ou proporções destas agindo nas diferentes partes do broto. A atividade máxima entre os pHs 4 e 6 evidencia tratar-se de uma enzima com atuação preferencialmente extracelular. A atividade específica enzimática foi maior na raiz, seguida das folhas e do hipocótilo. Com relação à atividade da raiz, houve diferença entre os tratamentos, em que a atividade dos brotos com seis dias foi maior que a de três, assim como nos germinados a 30 °C em relação a 25 °C. Os resultados indicaram que brotos cultivados a 25 °C e colhidos com três dias podem atenuar os efeitos indesejáveis da presença da peroxidase, como o escurecimento dos tecidos, e, assim, a vida útil do broto na pós-colheita. O tratamento térmico reduziu a atividade da peroxidase gradativamente com o aumento do tempo de inativação a 90 °C. A atividade peroxidativa dos brotos foi eliminada com o tratamento térmico a 90 °C/10 min. A aplicação de Ethrel a 20 ppm às 8 e 10 horas após o início da embebição resultou em melhor qualidade dos brotos, ou seja, maior espessura e comprimento do hipocótilo. A aplicação conjunta de Ethrel (20 ppm) e GA3 (500 ppm), nos mesmos períodos descritos anteriormente e produzidos a 25 °C + 2 °C, melhorou a espessura e comprimento do hipocótilo e produziu os brotos de cor mais clara. A qualidade do produto foi depreciada quando as sementes foram germinadas em temperatura constante de 30 °C, resultando em brotos muito tenros e com coloração mais escura em comparação com aqueles produzidos a 25 °C. Não houve diferença significativa entre as linhagens quanto ao teor de água dos brotos. O menor valor absoluto de 92,72% no teor de água das sementes foi observado na linhagem VC 3902A. O teor de água das sementes não influenciou o conteúdo de água dos brotos colhidos com cinco dias após a germinação.<br>The goals of the present work were to evaluate the physiological quality in seeds of mung bean, the influence of ethylene and gibberellic acid (GA3) on sprouts growth, relationship between peroxidase activity and browning and to determined the water content of seeds and sprouts. To evaluate the physiological of seeds it was used the germination test, accelerated ageing (42 °C/48 h and 42 °C/72 h), germination at low temperature (18°C) and electric conductance. The activity of peroxidase was determined in the root, hypocotyl and true leaf of the sprouts at different pHs (3, 4, 5, 6, 7, 8 and 9) from seeds germinated at 25 °C and at 30 °C, grown for 3 and 6 days, and inactivation of the enzyme by thermal treatment (90 °C) for different periods (0, 2.5, 5 and 10 min). The ethylene (Ethrel) and GA3 were applied alone or mixed at different stages of seed germination. The content of water in the seeds and sprouts were determined after drying at 70 °C until constant weight. It was tested the accesses Ouro Verde MG 2, VC 1973A, VC 5734A, V 3476, VC 4039A, VC 6148B16, VC 3890B and VC 3902A belonging to the germoplasm bank from EPAMIG/CTZM, but the peroxidase analysis was done in the cultivar Ouro Verde MG 2. The accelerated ageing and electric conductance were not efficient in evaluating the seeds vigor, but the germination at low temperature was able to evaluate it. The peroxidase activity was represented by similar curves in all tissues analyzed. The activity was lower at pHs 3 and 9, and higher at pHs 4 and 6. The highest activities were present at pHs 4 and 5 for roots, and pHs 5 and 6 for leaf and hypocotyl. These data suggest that there are different isoforms or proportions of peroxidase acting in the different parts of the sprout. The maximum activity between pHs 4 and 6 suggest that the peroxidase is present mainly outside the cell. The specific activity of the enzyme was higher in the root, followed by leaves and hypocotyl. In the roots, the peroxidase activity was higher in sprouts grown for 6 days at 30 °C compared to those grown for 3 days at 25 °C. Sprouts grown for 3 days at 25 °C showed less browning due to lower peroxidase activity, increase the shelf life. Thermal treatment reduced the peroxidase activity at 90 °C proportional to the length of treatment. The peroxidative activity was eliminated when the sprout extract was treated at 90 °C/10 min. The use of 20 ppm Ethrel at 8 and 10 hours after the beginning of germination resulted in better quality for the sprouts or thicker and longer hypocotyls. The combined treatment with 20 ppm Ethrel and 500 ppm GA3, at 25 °C improved the thickness and length of the hypocotyl, and resulted in lighter color. The quality of the sprout was reduced when the seeds were germinated at 30 °C, resulting in softer and darker sprouts, compared to those grown at 25 °C. There was not difference among the accesses regarding the water content of the sprouts. A lower value of 92,72% was present in the seeds of VC 3902A. The water content in the seeds did not affect the water present in the sprout five days after germination.
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Ray, Pushpanjali. "Search for novel actinomycetes from soil as potential biocontrol agent against fungal root pathogens of phaseolus vulgaris (L.) vigna radiata(L.)." Thesis, University of North Bengal, 2017. http://ir.nbu.ac.in/hdl.handle.net/123456789/2575.
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Ramirez, Tamariz Gilmar. "Influencia del crecimiento de la plántula sobre el contenido de folato del frejolito chino (Vigna radiata) en ausencia de luz." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2021. https://hdl.handle.net/20.500.12672/17053.
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El frejolito chino (Vigna radiata), legumbre considerada como
“perla verde”, es poco consumido en Perú debido a limitados estudios realizados
en este país acerca de sus nutrientes, compuestos bioactivos y efectos benéficos
para la salud. El frejolito chino es consumido en Perú generalmente como
plántula denominada comúnmente “germinado”, sin embargo, esta investigación
destaca la diferencia entre germinado y plántula, además, establece un
precedente en Perú acerca del contenido de folato en frejolito chino. La investigación
determina la influencia del crecimiento de la plántula sobre el contenido de
folato del frejolito chino (Vigna radiata) en ausencia de luz. Materiales y
métodos: Investigación experimental con semillas (testigo) y plántulas (grupo
experimental). Se utilizaron semillas de Vigna radiata. Las semillas fueron
germinadas para favorecer el crecimiento de las plántulas en ausencia de luz a
temperatura ambiente y humedad relativa. Posteriormente, se determinó el
contenido de folato en semillas y plántulas por método microbiológico (AOAC).
Encuentra la longitud de las semillas fue 0.4 cm y la longitud de las plántulas
fue 1.0 cm, 12.05 cm y 22.35 cm. El contenido de humedad en semillas fue 9.22%
y en plántulas fue 55.13%, 92.02% y 95.80%. El contenido de folato en semillas
fue 24.75 µg/100g y en plántulas no se pudo detectar folato. Concluye que se
determinó el contenido de folato en las semillas, sin embargo, no se pudo
determinar el contenido de folato en las plántulas de frejolito chino (Vigna radiata)
que crecieron en ausencia de luz por método microbiológico con Streptococcus
faecalis (ATCC 8043).
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Na, Jom Kriskamol [Verfasser], Karl-Heinz [Akademischer Betreuer] Engel, and Wilfried [Akademischer Betreuer] Schwab. "Metabolite profiling of sprouting mung beans Vigna radiata / Kriskamol Na Jom. Gutachter: Karl-Heinz Engel ; Wilfried Schwab. Betreuer: Karl-Heinz Engel." München : Universitätsbibliothek der TU München, 2012. http://d-nb.info/1031514694/34.
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Bourgault, Maryse. "Legume production in semi-arid areas: comparative study of the physiology of drought tolerance in common bean («Phaseolus vulgaris L.») and mungbean «(Vigna radiata (L.) Wilczek)»." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40664.
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Context: Approximately one billion people live in semi-arid and arid regions, and of these about 40% live on less than a dollar a day. Legumes crops are an important component of sustainable agriculture, but they are often grown under intermittent or terminal droughts. Thus, improving drought tolerance in legumes has the potential to improve food security and sustainability of agricultural systems. Objective: This study compares the response of two legume crops, common bean (Phaseolus vulgaris L.) and mungbean (Vigna radiata (L.) Wilczek), to water stress conditions under field and controlled-environment conditions. Methods: Field experiments were conducted in the Fergana valley, Uzbekistan, and controlled environment experiments were conducted at the Macdonald campus of McGill University, Ste-Anne-de-Bellevue, Canada, and at the Hermitage Research Station in Warwick, Australia. Results: Our results demonstrate that alternate furrow irrigation maintains yields, yet decreases water applied by 25%. In addition, mungbean showed the highest yields in the moderate deficit irrigation treatment in 2003 and severe deficit irrigation treatment in 2004 under field conditions in Uzbekistan. Common bean also showed a capacity to maintain yields under moderate deficit irrigation in both years. Further characterization of the legume responses to water deficit stress in controlled-environment experiments indicated that mungbean’s higher tolerance is attributable to higher transpiration efficiency, a more conservative water use in the vegetative stage, and a higher root-to-shoot ratio when compared to common bean. Root characteristics might also play an important role, although we have observed a large variability between genotypes. An additional field experiment in Uzbekistan demonstrated that an early maturing Canadian soybean cultivar could be grown after the harvest of winter wheat, and thus contribute to food security. It has also been demonstrated that i<br>Contexte : Environ un milliard de personnes vivent dans les régions arides et semi-arides, et 40% d’entre eux vivent avec moins d’un dollar par jour. Les cultures de légumineuses sont une part importante de l’agriculture durable, mais ces cultures sont souvent produites dans des conditions de sécheresse intermittente ou terminale. Améliorer la tolérance des légumineuses à la sécheresse peut donc augmenter la sécurité alimentaire et la durabilité des systèmes agricoles. Objectif : Cette étude compare la réponse de deux légumineuses, le haricot commun (Phaseolus vulgaris L.) et le haricot doré (Vigna radiata (L.) Wilczek), lors de conditions de stress hydrique imposées au champ et en environnement contrôlé. Méthodes : Les expériences au champ furent conduites dans la vallée de Fergana, en Ouzbékistan, et les expériences en environnement contrôlé furent conduites au campus Macdonald de l’Université McGill, à Ste-Anne-de-Bellevue, Canada, et à la station de recherche Hermitage, à Warwick, Australie. Résultats : Nos résultats démontrent que l’irrigation alternante maintient les rendements tout en diminunant de 25% l’apport en eau. De plus, le haricot doré a démontré les meilleurs rendements sous les traitements d’irrigation déficitaire moyen en 2003 et sévère en 2004 dans les expériences au champ. Le haricot commun a également démontré la capacité de maintenir ses rendements sous traitement d’irrigation déficitaire moyen les deux années. Une caractérisation plus poussée de la réponse des légumineuses au stress hydrique en milieu contrôlé a indiqué que la tolérance accrue du haricot doré est attribuable à une meilleure efficacité transpirationelle, une utilisation plus limitée de l’eau lors de la période végétative, et une proportion plus élevée de biomasse en racines lorsque comparé au haricot commun. Les caractéristiques racinaires semblent également jouer un rôle important,$
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NEVES, Ariana Morais. "Adubação verde com feijão mungo na viabilidade agroeconômica da hortelã." Universidade Federal de Campina Grande, 2017. http://dspace.sti.ufcg.edu.br:8080/jspui/handle/riufcg/2175.
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Previous issue date: 2017-12-08<br>O uso de adubos verdes constitui em alternativa viável para ser utilizado pelos agricultores que produzem em sistema orgânico, dado os grandes benefícios que a prática oferece. Dois experimentos foram conduzidos na Fazenda Experimental Rafael Fernandes, no distrito de Alagoinha, zona rural de Mossoró-RN, no período de agosto de 2016 a março de 2017, com o objetivo de estudar adubação verde com feijão mungo na viabilidade agroeconômica da hortelã. O delineamento experimental utilizado foi o de blocos completos casualizados em esquema fatorial 4 x 2, com três repetições. O primeiro fator foi constituído pelas diferentes densidades de semeadura do feijão mungo (50; 100; 150 e 200 plantas m-2) e o segundo fator pelas formas de manejo da biomassa do feijão mungo (incorporado e em cobertura). O cultivar da hortelã utilizada foi a Mentha piperita. As características avaliadas para esse vegetal em primeiro cultivo e na rebrota foram as seguintes: altura da biomassa, massa fresca, número de molhos, massa seca, teor e rendimento de óleo. Também foram utilizados indicadores econômicos, tais como: custo de produção, renda bruta, renda líquida, taxa de retorno e índice de lucratividade. No primeiro cultivo, a densidade de semeadura de 150 plantas m-2 contribuiu para o maior incremento na massa fresca e número de molhos com valores de 2.508,3 g m-2 e 30,8 unidades m-2 respectivamente, já para o rendimento de óleo a densidade de 200 plantas m-2 obteve um melhor resultado com valor e 1,24 g m-2. Em relação às formas de manejo (incorporado e em cobertura) não houve diferença estatística para massa fresca, número de molhos e rendimento de óleo. Para a rebrota da hortelã, a densidade de semeadura de 50 plantas m-2 de feijão mungo acarretou o maior incremento nas características de massa fresca e número de molhos, com valores de 922,6 g m-2 e 9,2 unidades m-2, mutuamente. Para o rendimento de óleo (0,78 g m-2), a melhor densidade de semeadura foi de 100 plantas m-2 de feijão mungo. Em relação às formas de manipulação (incorporado e em cobertura) observou superioridade da forma de manejo em cobertura para massa fresca, número de molhos e rendimento de óleo. A melhor eficiência econômica foi observada na densidade de semeadura 200 plantas m-2 com renda bruta (R$ 4.579,80), renda líquida (R$ 2.543.58) e índice de lucratividade (54,9 %). Para a taxa de retorno (R$ 2,70) na densidade de 150 plantas m-2. Nas formas de manejo da biomassa (incorporado e em cobertura), observou diferença estatística somente para a taxa de retorno na densidade de 150 plantas m-2, com valores de R$ 2,7 e 2,2, respectivamente. A utilização do feijão mungo mostrou eficiência econômica como adubo verde no cultivo da hortelã.<br>The use of green fertilize constituted in a viable alternative to be utilized by farmers that produce on organic system, due to the great benefits which the practice offers. Two experiments were conducted at experimental Rafael Fernandes Farm, in Alagoinha district, Mossoró-RN countryside, in the period from August 2016 to March 2017, with the aim to study green fertilizer with bean mung in agroeconomical of mint. The experimental delineatment utilized was complete blocks causalized on factorial scheme 4 x 2, with three repetitions. The first factor was constituted by differents sowing densities of bean mung (50; 100; 150 and 200 plants m-2) and the second factor by forms of management of biomass bean mung (incorporated and on covering). The cultivar of mint utilized was the Mentha piperita. The characteristics evaluated for the mint in the first cultivation and in the regrowth were as follow: biomass hight, fresh mass, number of sauces, dry mass, substance and income oil. We also utilized economic indicators, such as: cost production, gross income, net income, return rate and profitability index. In the first cultivation, the sowing density of 150 plants m-2 contributed for the major increase in the fresh mass and number of sauces, with values of 2,508.3 g m-2 and 30.8 units m-2 respectively, about the yield of oil, the density of 200 plants m-2, it got a best result with value of 1.24 g m-2. In concern the management forms (incorporated and in covering), there wasn’t statistical difference to fresh mass, number of sauces and income oil. For the regrowth of mint, the sowing density of 50 plants m-2 of bean mung caused the major increase in the characteristics dry mass and number sauces with values of 922.6 g m-2 and 9.2 units m-2 respectively. For oil income (0.78 g m-2), the best sowing density was 100 plants m-2 of bean mung. In concern management forms (incorporated and on covering) we observed superiority of management form on covering for fresh mass, number of sauces and oil income. The best economical efficiency was observed on sowing density 200 plants m-2 with gross income (R$ 4,519.80), net income (R$ 2,543.58) and profitability index (54.9 %). For rate return (R$ 2.70) on density of 150 plants m-2. In the management forms of biomass (incorporated and in covering), observed only statistical difference to a rate return on density of 150 plants m-2, with values of R$ 2.7 and 2.2, respectively. The utilization of bean mung showed economical efficiency like green fertilizer in the cultivation of mint.
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Miyagi, Mikiko. "Exploitation of bacterial artificial chromosome (BAC) libraries to enhance the efficiency of genome mapping." Thesis, Queensland University of Technology, 2002. https://eprints.qut.edu.au/37140/6/37140_Digitised%20Thesis.pdf.
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NEVES, Ana Paula Morais. "Viabilidade agroeconômica da alface (Lactuca sativa L.)." Universidade Federal de Campina Grande, 2016. http://dspace.sti.ufcg.edu.br:8080/jspui/handle/riufcg/818.
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Previous issue date: 2016-11-29<br>Uso de leguminosas como adubo verde é uma prática bastante consolidada, pelo fato de estar adicionando ao solo material vegetal rico em nitrogênio. O experimento foi conduzido na Fazenda Experimental Rafael Fernandes, no distrito de Alagoinha, zona rural de Mossoró-RN no período de agosto de 2015 a janeiro de 2016, com o objetivo de avaliar a Presença e ausência do feijão mungo sob doses de esterco bovino na viabilidade agroeconômica da alface. O delineamento experimental utilizado foi de blocos completos casualizados com os tratamentos arranjados em esquema fatorial 4 x 2, com quatro repetições. O primeiro fator foi constituído das doses de esterco bovino (1,0; 2,0; 3,0; 4,0 kg m-2 de canteiro) e o segundo fator foi constituído pela presença e ausência do adubo verde (feijão mungo). A cultivar da alface utilizada foi a “Regina”. Foram avaliadas as seguintes características: altura de planta, número de folhas planta-1, diâmetro da cabeça, produção de alface e massa seca de alface. Foram determinados alguns indicadores econômicos tais como: custo de produção, renda bruta, renda líquida, taxa de retorno e índice de lucratividade dos dois cultivos. Não houve interação entre os fatores-tratamentos para as características de produção, com produção de alface de 87,8 kg/100 m2. Houve diferença estatística no fator presença e ausência do feijão mungo com valores médios de 81,4 e 67 kg/100 m2 de alface, respectivamente. A maior eficiência econômica no cultivo da alface se deu na presença do feijão mungo na quantidade
de 3,0 kg m-2, com renda bruta de 3343,75, renda líquida de 1582,40, taxa de retorno de 1,90 e índice de lucratividade de 43,42%. A utilização de feijão mungo no cultivo da alface
constitui-se em uma opção viável para ser utilizado pelo agricultor.<br>The use of legumes as green manure is a well-established practice, because it is adding
nitrogen-rich plant material to the soil. The experiment was conducted at the Fazenda
Experimental Rafael Fernandes, in the district of Alagoinha, rural area of Mossoró-RN, from August 2015 to January 2016, with the objective of evaluating the Presence and absence of mung bean under doses of bovine manure in the agroeconomic viability of lettuce. The experimental design was a randomized complete block with treatments arranged in a 4 x 2 factorial scheme, with four replications. The first factor consisted of bovine manure (1.0, 2.0, 3.0, 4.0 kg m-2 of bed) and the second factor was the presence and absence of green mango (mung bean). The lettuce cultivar used was the "Regina". The following characteristics were evaluated: plant height, number of plant-1 leaves, head diameter, lettuce production and lettuce dry mass. Some economic indicators were determined such as: production cost, gross income, net income, rate of return and profitability index of the two crops. There was no interaction between the treatment factors for the production characteristics, with lettuce production of 87.8 kg / 100 m2. There was a statistical difference in the presence and absence of mung beans, with mean values of 81.4 and 67 kg / 100 m2 of lettuce, respectively. The highest economic efficiency in lettuce cultivation occurred in the presence of mung beans in the amount of 3.0 kg m-2, with gross income of 3343.75, net income of 1582.40, rate of return of 1.90 and index of profitability of 43.42%. The use of mung beans in lettuce cultivation is a viable option for the farmer to use.
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WU, JIA-JIN, and 吳珈瑾. "Functionality of mung bean (Vigna radiate L.) hydrolysates fermented by probiotic." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/55890434315175129025.
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碩士<br>國立高雄海洋科技大學<br>水產食品科學研究所<br>104<br>This study aimed to improve the functionality of mung bean, sample was hydrolyzed by cellulase and amylase, and further fermented by Lactobacillus johnsonii BCRC 17010 or Lactobacillus plantarum subsp. BCRC 10069. After 2 hr hydrolysis at 50oC by the combination of 1% cellulase and 1% α-amylase and further 24 hr fermentation with Lactobacillus johnsonii BCRC 17010 or Lactobacillus plantarum subsp. BCRC 10069 at 37oC, the LAB counts increased to 9.36 and 8.59 log CFU/mL, and the pH declined to 3.77 and 4.11, respectively. Scanning electron microscope (SEM) photograph indicated that the obvious break occurred in fibers after hydrolysis and fermentation. Increases in the amounts of the total phenolic, flavonoid and anthocyanin content and total amino acid content of hydrolysates and fermented samples were observed, compared with those of non-hydrolysed samples. These phenomena suggested the release of bionutrients occurred after hydrolysis or fermentation process. In hydrolyzed and further fermented samples, significant decreases in half maximal inhibitory concentration (IC50) on ABTS+˙ radical scavenging (0.47~1.52 mg/mL), α-amylase (1.89~2.14 mg/mL) and α-glucosidase (35.85~45.89 mg/mL) were observed. Gas chromatography-mass spectrometry analysis revealed that the major components were maltol and catechol. The IC50 on ABTS+• radical scavenging were 1.44 and 2.52 μg/mL, respectively. The fermented mung bean hydrolysates maintained 9.13~9.15 log CFU/mL (97.64%), 8.13~8.20 log CFU/mL (95.37%) in acid resistance (PBS at pH 3.0) and bile tolerance tests, respectively. According to the Ames tests, obtained, no matter whether the activating metabolites were added or not, the fermented products did not cause the genomic toxicity on the tested microbes ( Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535). The inhibition of LPS-induced in murine macrophage cell (RAW 264.7) were used to investigate the anti-inflammatory activities of the hydrolysates and their fermented samples. Inhibition in the releasing of NO (36.80~90.40%), interleukin 1β (49.26~72.29%) and interleukin 6 (17.78~93.75%) were obtained, respectively. These data suggested that hydrolysis and fermentation have high potential to promote the releases of functional components, which could subsequently improve the functionality of mung bean.
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Lin, Chia-Hui, and 林嘉慧. "Characterization of a Novel Y2K-type Dehydrin VrDhn1 from Mungbean (Vigna radiate L.)." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/41134026396992997187.
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博士<br>國立清華大學<br>生物資訊與結構生物研究所<br>101<br>A novel dehydrin gene (VrDhn1) was isolated from an embryo cDNA library of Vigna radiata (L.) Wilczek (mungbean) variety VC1973A. The intronless VrDhn1 gene encodes a protein belonging to the Y2K-type dehydrin family. VrDhn1 protein accumulated in embryos and cotyledons during seed maturation and disappeared 2 days after seed imbibition (DAI). The expression of VrDhn1 mRNA and accumulation of VrDhn1 protein were at high levels in mature seeds, but neither mRNA nor protein was detected in mungbean vegetative tissues under normal growth conditions. VrDhn1 mRNA level was extremely high in mature seeds and decreased to ~30% at 1 DAI, and was not detectable at ~7 DAI. Tissue dehydration, salinity and exogenous abscisic acid (ABA) markedly induced VrDhn1 transcripts in plants as measured by quantitative real-time reverse transcription-PCR (qRT-PCR). VrDhn1 protein was not detected using immunoblots in seedlings under stress treatments. VrDhn1:GFP fusion protein is degraded in transgenic Arabidopsis under normal condition, but is more stable under dehydration. In mature seeds or 1 DAI seedlings, VrDhn1 proteins were immunolocalized in the nucleus and cytoplasm. VrDhn1 exhibited low affinity of non-specific interaction with DNA using electrophoretic mobility shift assays (EMSA), and the exogenous addition of Zn2+ or Ni2+ stimulated interaction. Phosphorylation occurs on threonine and tyrosine residues of VrDhn1 in mungbean seeds. The His-tagged VrDhn1 (30.17 kDa) protein showed a molecular mass of 63.1 kDa on gel filtration, suggesting a dimer form. This is the first report showing that a Y2K-type VrDhn1 enters the nucleus and interacts with DNA during seed maturation.
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Yu, Yueh-hsiang, and 游約翔. "Substrate specificities of Hsc70s of Vigna radiata." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/09698364834473851860.
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碩士<br>國立清華大學<br>生命科學系<br>90<br>The 70 kilodalton heat shock cognate protein (Hsc70) is a constitutively expressed member of the molecular chaperones which serve to prevent protein misfolding and aggregation in the crowded environment of the cell. Hsc70 cooperates with cochaperone Hsp40, and functions by binding and releasing the exposed extended polypeptide of nascent and nonnative proteins, in the ATP-dependent manner. Binding specificities of Hsc70s to their substrates are quite different among bacteria, yeast, vertebrate and plant. To investigate the interactions and specificity between Hsc70 and its substrates, the carboxyl-terminal 30 kDa of three mung bean (Vigna radiata) Hsc70s were expressed and used as target proteins to select Hsc70-binding heptapeptides using phage display screening. Three heptapeptides of high frequencies were selected with the target proteins: KVWVLPI for VrHsc70-1, KLWVIPQ for VrHsc70-2, KLWVIPQ and YAPLSRL for VrHsc70-3. The target binding was confirmed by ELISA analysis. These three heptapeptides were subject to search for the homologous proteins. Our results suggested that VrHsc70-1 may assist the nonnative or newly synthesized proteins to fold correctly, and VrHsc70-2 and 3 may bind substrates during de novo synthesis stages. To study the binding patterns of three significant heptapeptides to VrHsc70s, InsightII program was used to docking each heptapeptide into its target VrHsc70. The lowest-energy binding models were inspected and a similar binding mode of VrHsc70s to KVWVLPI, KLWVIPQ and YAPLSRL was obtained.
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Thuan, Nguyen Dat. "Expression and inheritance of traits in wild mungbean (Vigna radiata ssp. sublobata) x cultivated mungbean (v. Radiata ssp. radiata) hybrids." Thesis, 2011. https://researchonline.jcu.edu.au/31508/1/31508_Thuan_2011_thesis.pdf.
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Mungbean (Vigna radiata (L.) Wilczek ssp. radiata) is an economically important crop in Asian countries where breeding research is being undertaken to improve varietal adaptation, yield and seed quality. The wild mungbean (ssp. sublobata) is a potentially useful adjunct to breeding, as the wild accessions possess traits that confer adaptation in their natural environments. The wild accession ACC 87 collected near Townsville has been identified as being perennial, a potentially useful trait for forage crop improvement. Accession ACC 1, from Mackay is very late flowering and was reported to possibly have a long juvenile (LJ) trait similar to that found in soybean. Before these and other potentially useful wild traits can be exploited, information is needed on their inheritance. Therefore, a study was conducted to examine the inheritance of traits in four hybrid cultivated X wild mungbean populations. The study examined the expression of traits in the parental plants, and the F1, F2, BCP1 and BCP2 progeny generations, when grown under controlled conditions in pots. The four genetic populations had been created by hybridizing using two cultivated mungbean varieties, Berken and Kiloga, with each of two wild parents, ACC 1 and ACC 87.
Several morphological traits, including lobed leaflet shape, seed testa and hilum color, and plant habit were found to be under simple (qualitative) genetic control, with the wild type generally dominant. An exception was putative resistance to powdery mildew infection in the wild accessions, which appeared to be recessive. Many other traits like phenology, nodes per plant, seed yield and biomass were under quantitative genetic control. The perenniality trait in ACC 87 appeared to be under simple genetic control, with expression of perenniality due to two dominant complementary genes. In contrast, flowering in the two ACC 1 populations appeared to be quantitatively inherited, with no evidence of a LJ trait. There were many similarities in the genetic control of both qualitative and quantitative traits among the four hybrid populations, with only small differences due to the different cultivated parents. However, larger differences were apparent between the populations involving ACC 1 and ACC 87.
Estimates of narrow sense heritability were high for many of the qualitatively inherited traits, indicating high additive genetic variance for those traits, and thus the capacity for genetic gain through selection. Transgressive segregation occurred for most of the quantitative traits in one or more of the four crosses, indicating the potential value of the wild germplasm in broadening the phenotypic range available to plant breeders. Several phenotypic and genotypic interrelations found between many of the wild traits among the four crosses. In particular, there were several significant genetic correlations among quantitative traits, indicating that selection for one of the traits should result in genetic advance in the other.
The study confirmed earlier research that Australian accessions of the wild mungbean can be considered part of the primary gene pool of the cultivated mungbean. Consequently, the wild accessions provide an additional source of triats potentially useful for mungbean improvement. The study also established that traits of possible commercial interest, perenniality and powdery mildew resistance, were qualitatively inherited and thus should be readily transferrable into cultivated varieties. While the study failied to identify the presence of a LJ trait, it suggested that the wild germplasm could be a useful source of lateness genes for breeding vegetatively vigorous forage or cover crop varieties of mungbean.
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Peng, Shu-Kai, and 彭書愷. "Cloning and characterization of a mungbean (Vigna radiata L.) starch phosphorylase cDNA." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/05958385857945258069.
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碩士<br>國立臺灣海洋大學<br>食品科學系<br>95<br>Starch is the major carbohydrate reserve in higher plants. In the starch biosynthetic pathway, starch phosphorylase (EC 2.4.1.1, SP) catalyzes a reversible reaction between synthesis and degradation of starch with bidirectional activities. Mungbean (Vigna radiata L.) starch is the most special that amylose content was 15-30% higher than normal level, which implied that SP plays an important role in amylose synthesis. Previous studies have identified the tryptic fragments of mungbean 105-kDa SP by MALDI-TOF and conducted immunological analysis. The objective of this study was to further clone the full-length cDNA of SP in order to investigate the characteristics and function of recombination SP in the future. Based on the internal amino acid sequence of N- and C-terminal of mungbean SP, degenerate primers were designed as gene-specific primers (GSP). The developing mungbean seed (Vigna radiata cv. Tainan no.5) was used as the material to extract its total RNA, and mRNA was purified and used as the template in RT-PCR. First, a 1819 bp sequence of a middle fragment was obtained, and then primers were designed from its internal sequence. The 5’-nucleotide conserved region sequences from fave bean, sweet potato and potato were used to design primers which were coupled with internal GSPs for RT-PCR amplification. Sequences containing the start codon ATG of 5’ 640 bp and 3’ sequences containing the stop codon TAG of 997 bp were obtained successfully. The full-length cDNA of the mungbean SP which possessed nucleotides of 2961 bp in length, containing complete open reading frame (ORF) that covers from start codon to stop codon was designated as Vrsp. Vrsp encodes a polypeptide of 987 amino acids with predicted molecular mass of 111 kDa and pI of 5.38. Putative protein sequences possess the motifs of starch binding site, L-78, catalytic site and pyridoxal phosphate (PLP) binding site. In addition, Vrsp includes the L-78 insertion sequence, it belongs to the known L-type isoform.
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Chen, Yu-Ting, and 陳郁婷. "Characterization and product of mungbean (Vigna radiata L.) starch phosphorylase recombinant protein." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/58906698204988611440.
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碩士<br>國立臺灣海洋大學<br>食品科學系<br>100<br>Starch phosphorylase (SP; EC 2.4.1.1) is one of the enzymes for starch metabolism. The aims of this study were to optimize the expression of a potential recombinant mungbean (Vigna radiata) sp clone (pMAL-c2X-Vrsp-modify) in the E. coli system, and to investigate the enzymatic characteristics of the recombinant SP (rVrSP) in the synthesis direction. Items include stable product formation condition, enzyme kinetics, and analysis of the enzyme products. Evaluation of the optimal expression condition for rVrSP showed that the cells grown at 37℃ until A600nm 0.6, then induced with 0.6 mM IPTG for 6 hrs at 20℃, the molecular size of the amount of the soluble MBP-rVrSP protein of 150-kDa was achieved. In situ zymogram staining of the MBP-rVrSP extract detected the activity of glucan synthesis, and the soluble enzyme was active at 4℃ for 30 days. Analysis of the 150-kDa band from either SDS-PAGE or from the activity gel slice of in situ staining, matched with gi|2506470 (α - 1,4 glucan phosphorylase L isozyme) protein sequences of Vicia faba by LC/MS/MS. The optimal synthesis activity of the MBP-rVrSP extract in gel or in vitro solution were tested at different conditions to react for 2 hrs, and to detect glucan content by iodine reagent. It showed that enzyme is most active at pH 6.0 under 30℃ when reaction solution containing 0.8% or when the gel matrix containing 1.2% glycogen. The stable product formation is observed at 20-30℃. The steady-state kinetic parameters were determined in the 50 μg soluble MBP-rVrSP extract that the apparent Km and Vmax values for G-1-P are 5.5 mM and 0.3 A600nm, respectively. When 50 mM G-1-P was reacted with 500 μg soluble MBP-rVrSP extract at 1, 2, 3, 4, 5 and 6 hrs at 30℃, and the chromatogram of the enzyme products by HPLC showed the peak eluted at an earlier time as reaction 0-3 hrs, and the major products molecular mass is about 4500-4000 Da (DP 28-25). However, as reaction 4-6 hrs, the peak profiles consist of two major products about 3800 Da and 1200 Da, indicating the soluble MBP-rVrSP extract can synthesize and degrade the glucans, and the reaction directions are determined by the balance between the available G-1-P and inorganic phosphorous concentrations.
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Chia, Hsu Hung, та 許宏嘉. "Circular permutation on cysteine- stabilized αβ motif of Vigna radiata plant defensin 1". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/85t3v2.
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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>103<br>Mung bean (Vigna radiata) plant defensin 1 (VrD1) is the first reported plant defensin, which exhibits insecticidal activity against Callosobruchus chinensis (bruchid). VrD1 is a 46-residue basic peptide containing a cysteine-stabilized αβ (CSαβ) motif with four disulfide-bonds to stable its structure. Three dimensional structure determined by nuclear magnetic resonance (NMR) spectroscopy and alanine substitutions of noncysteine residues in VrD1 were done in our lab. Our previous studies showed that the loop L3 and the 310 helix played important roles in VrD1 insecticidal function. In this study, a protein engineering method – circular permutation (CP) – was employed on VrD1. The CP rearrangement in a protein is visualized as that the original termini of polypeptide are linked and new termini are created elsewhere. The CP rearranged proteins can be utilized to explore protein folding, stability of structure, and functions. Two CP-VrD1 proteins with residue M36 as the new N-terminus and linkers of one and two glycine (VrD1 CP36_G and VrD1 CP36_2G, respectively) were obtained and investigated. VrD1 CP36_G lost almost all structure and function, while VrD1 CP36_2G kept both characters. The length of linker for native termini may affect the protein folding, and our results revealed that the length of linker should be longer than the distance between the native N- and C-termini. VrD1 CP36_2G inhibited α-amylase although the new termini were created at the functional loop L3 and its IC50 was 1.93 μM while that of wild type VrD1 was 3.14 μM. So the important role of loop L3 may be to display the positively charged residues which participated in the electrostatic interaction between VrD1 and TMA.
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Shih, Yun-Chi, and 石韻琦. "Cloning and characterization of starch branching enzyme cDNA in mungbean (Vigna radiata L.)." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/70381090844490007521.
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碩士<br>中國醫藥大學<br>營養研究所<br>92<br>Abstract
Starch branching enzyme (SBE, EC 2.4.1.18) is one of the enzymes in the starch biosynthetic pathway. It catalyzes the formation of branches, which plays a vital role in amylopectin synthesis. The objectives of this thesis are to clone full-length cDNA of SBE and analyze its structure and characteristics. Based on database search, the conserved regions from published SBE genes were obtained and used to design gene-specific primers for cloning. First, the mRNA from developing mungbean (Vigna radiata, cv. Tainan no.5) was extracted and used as template in RT-PCR. The cDNA sequences of the amplified RT-PCR products, after comparing with GCG nucleotide database, demonstrates that partial cDNA of two distinct mungbean SBE isoforms (SBEII and SBEI) were found. Then, primers designed from internal sequences of SBEII and SBEI were used in cloning their 5’ and 3’ cDNA ends by 5’/3’ RACE (rapid amplification of cDNA ends). The full-length cDNAs of the two SBE isoforms were obtained successfully, which possess sequences of 2571bp and 2208bp in length (designated VrsbeII and VrsbeI), respectively. VrsbeII and VrsbeI have also been registered in GenBank with accession numbers of AY622199 and AY667492. They both contain complete open reading frame (ORF) that covers from start codon to stop codon. VrsbeII encodes a polypeptide of 856 amino acids with predicted molecular mass of 97 kDa and pI of 5.47. Whereas, VrsbeI encodes a polypeptide of 735 amino acids with predicted molecular mass of 84 kDa and pI of 6.35. Besides, their putative protein sequences possess the properties of the α- amylase family, including four active conserved region- HSHS/A S, GFRFDG VT, G/AEDVS and AESHDQ, and catalytic (β/α)8-barrel domain. When compared in database, both VrsbeII and VrsbeI showed substantial similarity to the SBEs of kidney bean and pea.
Furthermore, the identities between mungbean SBEII and SBEI at nucleic acid and protein levels are 59% and 56%, respectively. The deduced amino acid sequences from VrsbeII and VrsbeI via phylogenetic analysis is evident that they fall into two distinct gene families (family A and B). In conclusion, there are at least two different SBE isoforms involved in starch biosynthesis during the development of mungbean.
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呂曉鈺. "Cloning and characterization of mungbean (Vigna radiata L.) granule-bound starch synthase cDNA." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/81563205899504005617.
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Chen, Mei-Yun, and 陳美雲. "Purification and Characterization of an Asparaginyl Endopeptidase from Mung bean（Vigna radiata）seedlings." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/32715798981981951815.
Full textAbstract:
碩士<br>國立臺灣海洋大學<br>食品科學系<br>94<br>Abstract
To understand the physiological function of VrPE-1, a putative asparaginyl endopeptidase, in the cotyledons of germinated mung beans (Vigana radiate), a polyclonal antibody against VrPE-1 and an enzymatic activity of asparaginyl peptidase with Z-Ala-Ala-Asn-MCA as a substrate were utilized for the analysis of the expression and the purification of VrPE-1 from germinated mung beans. By Western blot analysis, VrPE-1 occurs in cotyledons, hypocotyls and roots, but not in leaves, of germinated mung beans on 5 DAI (days-after-imbibition), and its expression in cotyledons reaches a maximum on 5 DAI.
Ammonium sulfate fraction (0-45% saturation) of the crude protein extract from 5-DAI cotyledons was chromatographed on a Q-Sepharose column. The fractions exhibiting asparaginyl peptidase activity were pooled and further chromatographed on a butyl-Sepharose 4 column. The chromatogram of butyl-Sepharose 4 showed a single peak of asparaginyl peptidase activity.
The enzymatically active fractions were collected and pooled for further biochemical characterization. The resulting pooled fraction showed several bands on SDS-polyacrylamide gel and only one band with a molecular mass of 18 kDa on Western blot. The pooled fraction had storing pH optimum around 5.0 for the asparaginyl peptidase activity with Z-Ala-Ala-Asn-MCA as a substrate. The fraction showed about 50% enzymatic inhibition with 10 μM PMSF, 1 �嵱 leupeptin or 10 μM NEM, and small effect with 1 �嵱 E-64 inhibitor.
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Chan, Sam-I., and 陳心儀. "Characterization of Mungbean (Vigna radiata L.) Starch Branching Enzyme II in E. coli host." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/11662093372678656379.
Full textAbstract:
碩士<br>國立臺灣海洋大學<br>食品科學系<br>97<br>Starch branching enzyme (SBE, EC 2.4.1.18) is one of the enzymes in the starch biosynthetic pathway and it plays a vital role in the formation of amylopectin, the main component of the starch molecule. The chemical fine structure changed by SBE would affect the physical property and function of starch. The objective of the thesis was to optimize the expression of a potential recombinant SBEII clone and characterize the active recombinant enzyme to be used in modifying the starch molecular structure. The constructed pET21b-VrSBEⅡwas first transformed into BL21 (DE3) expression host and induced with 0.2 mM IPTG for 5 hours at 37℃, SDS-PAGE analysis showed the molecular size of the recombinant protein was 108 kDa. This rVrSBEⅡwas expressed as a soluble protein with crude enzyme activity of 0.25 U/mg and the activity was enriched 27 fold by Ni-NTA affinity chromatography. To increase the solubility and activity of the enzyme, the clone was further expressed in Origami B host and induced under the same induction condition. Improvement of rVrSBEⅡwas not only the solubility but the crude activity of 0.46 U/mg has increased to 14.9 U/mg after purification. The enzyme was most active at pH 7.0 at 30℃and relatively stable up to 30℃.More than 90% of the original activity remained after incubated at pH 7.0-9.5. When 1 mg/ml amylose substrate was used to react with 6�n�慊 of purified rVrSBEⅡat 30℃, there was a time-dependent decrease of the absorbance at OD660 and became steady after 90 minutes. GPC-HPLC analysis of the enzyme products showed the peak eluted at an earlier elution time as reaction proceeded, indicating the formation of branching and size enlargement by rVrSBEⅡin the product. The present study shows that the pET21b-VrSBEⅡclone was able to be expressed as a biologically functional enzyme in E. coli system and the protein solubility and activity were improved by expressing in Origami B host. Basic characteristics of rVrSBEⅡand the product were established.
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Chang, Wei-Chi, and 張瑋齊. "Construction of a mungbean (Vigna radiata L.) starch phosphorylase cDNA in E. coli system." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/20361438425510788682.
Full textAbstract:
碩士<br>國立臺灣海洋大學<br>食品科學系<br>97<br>Starch phosphorylase ( SP, EC 2.4.1.1 ) is commonly found in plants and is one of the vital enzymes in the starch metabolic pathway. It catalyzes a reversible reaction between synthesis and degradation of starch with bidirectional activities. Previous studies have identified the fragments of mungbean 105-kDa SP by MALDI-TOF, conducted immunological analysis and amplified the full-length cDNA fragment of mungbean (Vigna radiata L.) SP (named VrSP). The objective of this study was to clone the sequence into an expression vector and further express into a biological-active recombinant protein in E. coli system. Its 3-D structure and functional features were predicted in silico by SWISS-Model. One matched template, rabbit muscle phosphorylase with potent inhibitor (IWW2A), predicted the N-terminal of VrSP from 92 residue to 411 residue, including partial starch-binding site. The other template, rabbit muscle phosphorylase (2GJ4A), predicted the C-terminal of VrSP from 585 residue to 984 residue, including catalytic site (711-727). VrSP insert was prepared by PCR using primers designed with EcoRI and SalI sites in the flanking region, and was ligated into the parallel sites on pMal-C2X vector, followed by transforming into Novablue cloning host and screening. Insert size of one recombinant plasmid was nearly closed to the expected 2.9 Kb, however after sequencing, 97% of its sequence is matching to the DNA sequences in E. coli cloning host. In addition, the other two major groups of recombinant plasmid had 2.2 kb and 1.4 kb insert fragments in pMal-C2X vector. It showed that the sequence of VrSP itself may cause recombination with the host E. coli chromosomal DNA during the growth of these recombinant clones.
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Ku, BI-Jan, and 古璧甄. "Study on Cultivation of Mungbean (Vigna radiata (L.) Wilczek) Sprout with High Phytochemical Content." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/95033178855258254465.
Full textAbstract:
碩士<br>國立臺灣大學<br>園藝學研究所<br>103<br>Two isogenic lines of mungbean, bruchid-resistant VC6089A and bruchid-susceptible VC1973A, and Sunright mungbean (Sunright Foods Co.) were used as experimental material to study the optimal environmental condition of cultivating mungbean sprout with high phytochemicals contents in the dark. Mungbean seeds were soaked at 20˚C, 26˚C and 32˚C for 0、4、8 and 12 h. Seed dry weight decreased as the soaking time extending to 12 h. Among 4 planting densities, 7 g·cm-2 treatment showed the highest harvest index, longer hypocotyl length and higher total flavonoids content in VC6089A and VC1973A sprouts. Total phenolics content in mungbean sprouts was highly positively related to harvest index (r=0.74***), so did the total phenolics content to total flavonoids content (r=0.77***). Among forcing pressures of 0, 100, 200, 300 and 400 g·51.84 cm-2, VC1973A mungbean sprout cultivated under 400 g·51.84 cm-2 showed shorter and thicker hypocotyl also shorter root. In forcing pressure experiment, correlation coefficient (r) of total flavonoids content to α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP) were 0.82*** and 0.64***, respectively. Among 6 combinations of temperatures based on 16 h/8 h cycle, fresh weights of VC6089A sprouts were the highest at 29˚C/23˚C and 26˚C/26˚C, while Sunright and VC1973A sprouts at 26˚C/26˚C and 29˚C/23˚C, respectively. Soluble solids and soluble protein contents of mungbean sprouts were both affected by the cultivating temperature combinations. VC1973A sprouts has the highest content of total flavonoids and total phenolics than other 2 entries. Comparing to 0, 10 and 25 mM NaCl solution, sprouts cultivated in 15 mM NaCl solution exhibited higher fresh weight and total flavonoids and total phenolics contents than under 0, 10, 20 and 50 mM NaCl treatments. VC1973A sprouts also showed higher Fe2+ chelating ability in 15 mM NaCl treatment. In NaCl experiment, sprout fresh weight was positively related to soluble sugar content (r=0.83***), total flavonoids content (r=0.84***), total phenolics content (r=0.85***) and DPPH scavenging ability (r=0.85***) in NaCl experiment. Contents of total phenolics and flavonoids contents could be reference values of DPPH radical scavenging activity and FRAP. Cultivation sprouts with 0, 10, 25, 50, 75 and 100mM 5 glucose solution, 50 mM treatment could increase ascorbic acid and total flavonoids in mungbean sprouts. Sprouts grown in 100 mM glucose solution had more soluble solid and protein. In conclusion, VC1973A sprouts had higher fresh weight and phytochemicals contents than VC6089A and Sunright sprouts. The mungbean sprout would have higher total flavonoids (0.38 mg·g-1DW) and total phenolics (11.1 mg·g-1DW) contents and better Fe2+ chelating ability (84.8 % of 50 mg DW·mL-1), DPPH scavenging ability (54.1 % of 50 mg DW·mL-1), and FRAP (150.9 µmol FeSO4·g-1DW), and higher fresh weight (54.5 g FW·51.84 cm-2). VC1973A seeds are suggested to be cultivated with 7 g·cm-2 sowing density, 400 g·51.84 cm-2 forcing pressure, 15 mM NaCl solution at 29˚C 16 h/23˚C 8 h in the dark.
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Wang, Jyun-Jheng, and 王鈞正. "Expression of mungbean (Vigna radiata L.) starch phosphorylase recombinant protein in E. coli system." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/70346398588369100221.
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Chen, Shu-Ling, and 陳淑玲. "Chilling Stress Effects the Plastid Development and Gene Expression in mungbean （Vigna radiata L. ）seedlings." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/05510974194634430520.
Full textAbstract:
博士<br>國立臺灣大學<br>植物學研究所<br>87<br>Mungbean ( Vigna radiata L. cv Tainan no. 5 ) is a chilling-sensitive plant. The growth of etiolated mungbean seedlings was very slowly at 10 oC in the light. The leaves continued to grow and finally expranded as those grown at 28oC in the light, but they did not turn to green. The development of plastids into chloroplasts in the etiolated leaves was retarded under chilling in the light condition when leaf ultrastructure was examined under T. E. M.. The synthesis of chlorophyll in the leaves were also retarded by 10 oC-chilling treatment. By SDS-PAGE and 2D-PAGE analyses of total leaf proteins, we found that some PS I and PS II proteins decreased dramatically especially in PSAA, PSAB and other thylakoidal proteins.
We also have been screened 10 oC-chilling sensitive genes from vcd01 to vcd10. The length of DNA fragment in vcd01 to vcd10 were 4, 3, 2, 7, 4, 6.5,2.1, 5.6 and 6Kb, respectively. These genes included psaC, psbB, psbC, psbD, psbE, psbF, psbJ, psbN, psbH, psbT, ndhA, ndhB, ndhD, ndhE, ndhG, ndhI, ndhH, petA, petB, petG, rpoA, rpl14, rpl16, rps7, rps15 and ycf5 genes. And all of 10 vcd genes have been sequenced completely. By means of northern blot analysis, the expression of psaA-psaB, ndhB, petB, rpl14, rpl16, and rpoA in the leaves were retarded at 10 oC in the light. Time course studies of retardation gene expression in above genes, from the experimental results showed that the psaA-psaB gene expression reduced immediately after 1 h of 10 oC chilling treatment. The ndhB, petA, rpl14 and rpl16 reduced after 4 h of chilling treatment. But the rpoA gene only reduced slightly after 7 h of chilling treatment. The chilling stress also caused the water deficient in the mungbean seedlings. By means of northern blot analysis, the down-regulated RNA expression in water stress were significantly different from these genes in chilling-sensitive gene expression. The amino acid sequence deduced from the nucleotide sequences of vcd01 to vcd10 shared significant homonology with other plant chloroplast proteins. The base composition, PI value, hydropathy and molecular weight of PSAC, PSBB, PSBE, PSBF, PSBJ, PSBN, PSBT, NDHD, PETA, PETG and YCF5 were also disscused in this study. The availability of vcd01 to vcd10 will facilate the physiological and biological studies in roles of plastid development and the mechanism of these gene expression under 10 oC chilling in the light.
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Chen, Yung-Che, and 陳詠哲. "Purification and Functional Analysis of the Bruchid Resistant Protein VrCRP from Vigna radiata VC 6089A." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/08554565764190812266.
Full textAbstract:
碩士<br>國立臺灣大學<br>農業化學研究所<br>89<br>Abstract
A cDNA named VrCRP (cysteine-rich protein of Vigna radiata) was previously isolated in our laboratory from an insect resistant nearly isogenic line of mung bean Vigna radiata VC6089A (Abbreviated as VC6089A) versus a susceptible isogenic line Vigna radiata VC1973A using suppressive substractive hybridization. The recombinant protein VrCRPDsp (Dsp represents signal peptide truncated) was shown to be highly toxic to one of the major bruchids of mung bean seeds Callosobruchus chinensis. In order to study the structure, properties and biological activities of naturally occurring VrCRP in VC6089A, VrCRP was purified directly from VC6089A by a procedure involving CM-Sepharose chromatography and Superdex Peptide HR10/30 gel filtration in FPLC system. This purification procedure was proved to be a simple and rapid method to isolate VrCRP from mung bean. Thirty-five microgram of highly purified VrCRP could be obtained from 1 g of mung bean seeds (0.0035%, w/w). The fractions containing VrCRP in the chromatography were recognized by the polyclonal anti-recombinant VrCRPDsp antiserum. A novel putative lipid transfer protein, designated as VrLTP, was also cross-reacted with the antiserum. The first 15 amino acids of the purified VrCRP determined by N-terminal Biosystems were completely consistent with the deduced amino acid sequence of VrCRP cDNA starting from Arg28. The results indicated that there is a signal peptide from Met1 to Ala27 in the full length VrCRP (73 amino acids), and the processed VrCRP contains 46 amino acids. The calculated molecular mass and pI value of the purified VrCRP were 5123 Da and 8.42, respectively. Based on amino acid sequence, molecular weight and pI value, VrCRP probably belongs to the g-purothionin-like family of plant defensins. Comparison of amino acid sequences of plant defensins and similarity dendrogram show that VrCRP appears to represent a novel subgroup of plant defensins. Plant defensins have been known as antimicrobial peptides/proteins. To our knowledge, VrCRP is the first plant defensin demonstrated to be also active against insect pests.
We also analyzed some of the biological activities of the purified VrCRP. In the in vitro translation system derived from wheat germ extract, about 50% of protein synthesis was inhibited by 5 mM VrCRP. The protein also inhibits the growth of a soil-borne pathogenic fungus, Rhizoctionia solani. Growth arrests of E. coli and a budding yeast, Pichia patoris, were also observed in media containing 5 and 10 mM VrCRP, respectively.
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Chiu, Shu Jun, and 邱淑君. "Studies on gene structure of mung bean (Vigna radiata L.) Vacuolar H+-ATPase A subunit." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/37437452062442227865.
Full textAbstract:
碩士<br>國立清華大學<br>輻射生物研究所<br>84<br>Vacuoles of higher plant cells contain two primary electrogenic
proton pumps, a vacuolar H+-ATPase (V-ATPase) and a H+-
translocating inorganic pyrophosphatase (V-PPase), for the
regulation of cell turgor, cytoplasmic homeostasis, and the
storage of metabolites. In this work, the structure of the mung
bean V-ATPase A subunit gene was investigated. In order to
clone the cDNA, primers were synthesized according to the
conserved sequences of cotton and carrot V-ATPase A subunit to
conduct polymerase chain reaction (PCR) using the total DNA
extracted from mung bean cDNA library as a template. The PCR
product was then employed as a probe to screen the constructed
mung bean cDNA library. The cloned V-ATPase A subunit cDNA
exhibits 83.8, 83.4, and 81.1% nucleotide homology to those of
cotton, carrot, and Brassica napus, respectively. The cDNA
sequence encodes 623 amino acids with a predicted Mr of
68,664and a predicted isoelectric point of 5.17. The amino acid
sequence of V-ATPase A subunit from mung bean seedlings shares
94.0~95.0% identity and 96.6~97.0% similarity to those from
carrot, cotton, and Brassica napus. Expression of the A subunit
gene was also investigated by Northern hybridization. It was
found that the leave expressed the most abundance of the
transcript, followed by hypocotyl and roots. Genomic Southern
analyses reveal a simple reaction pattern of the gene,
referring the lack of isoform for the A subunit. We also took
further step to explore the genomic structure of the A subunit.
To construct the DNA library, mung bean genomic DNA was
partially digested by Sau3AI, and fractionated by sucrose
gradient centrifugation. The 9 to 20 kb DNAs were ligated with
l/Dash II DNA and packaged. The constructed library was
screened using A subunit cDNA as a probe, and several positive
clones were obtained. Some of these clones contain both 5?and
3?regions of the gene.
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Chen, Li-Ru, and 陳麗如. "Comparative gene expression profiles of mungbean, Vigna radiata (L.) Wilczek, seedlings in response to cold." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/32766895851325010213.
Full textAbstract:
博士<br>國立清華大學<br>生命科學系<br>96<br>Mungbean (Vigna radiata (L.) Wilczek) is commonly used as human food. However, the genomic resources of this species available in databases are limited. This study has two sections, the expressed sequence tags (EST) related to early seedling development and chilling response are developed in the first part. Two mungbean varieties NM94 and VC1973A were obtained from the Asian Vegetable Research and Development Center-The World Vegetable Center (AVRDC). They differ in disease resistance and susceptibility to chilling temperatures. NM94 can maintain a better membrane integrity than VC1973A during seedling stage exposed to 4 degree C. To investigate the molecular mechanisms of the inherent chilling-susceptibility, the gene expression patterns in young seedlings of NM94 and VC1973A were compared in the second part of this thesis. A cDNA microarray containing 735 uniESTs was employed to profile the transcriptional changes during chilling stress and after recovery from chilling. The results show that a chilling exposure triggered the expression of a common set of CORs (cold regulated genes) in both NM94 and VC1973A. These CORs encode proteins that are involved in restructuring the protein synthesis apparatus, participating in protein trafficking and degradation, and synthesizing the stress protectants. The comparative expression profile indicates that the photosynthetic capacity and the cryoprotective proteins may be the key regulators of comparative toler55ance to chilling in NM94. Our data support the role of LTPs (lipid transfer proteins) in cryoprotection during chilling stress and resumption of plant growth after recovery from chilling.
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Chung, Pei-Shan, та 鍾佩珊. "Expression of mungbean (Vigna radiata L.) starch branching enzyme ІІ recombinant protein in E. coli system". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/55793443226754783040.
Full textAbstract:
碩士<br>國立臺灣海洋大學<br>食品科學系<br>95<br>Starch branching enzyme (SBE, EC 2.4.1.18) is a vital enzyme for amylopectin synthesis in the starch biosynthetic pathway. The aim of this thesis was to subclone VrsbeII of mungbean (Vigna radiata L. cv Tainan no. 5) into expression vector and produce active enzymes in the E. coli system. First, VrsbeII was constructed into pET-30 EK/LIC vector and transformed into E. coli NovaBlue host cells, hopefully full length sequence of the open reading frame could be obtained. However, there was only approx. 350 bp fragment could be stably maintained in pET-30 EK/LIC system and the unexpected outcome reoccurred. The sequence in the 350 bp was carefully examined and found astonishingly that two transposon-like 6-bp (CCAGTT) direct repeat sequences were in VrsbeII. Therefore, primers designed with Bam HІ and Not І sites which skipped one direct repeat sequence were used to prepare a 24 nucleotide shortened VrsbeII at the 3’-end by PCR. The redesigned insert fragment was ligated into the parallel sites on pET21b vector, followed by transforming into E. coli BL21 (DE3) cells, and expressed successfully as a soluble protein in the cytosol. The optimal expression condition for rVrSBEⅡwas evaluated that the cells were grown at 37℃ until OD600 to 0.6, then induced with 0.2 mM IPTG for 5 hrs, and the maximal crude enzyme activity of 0.25 U/mg was obtained. The crude enzyme was purified by HisTrapTM affinity column and the molecular size of rVrSBEⅡwas 108-kDa whose activity has enriched 25.5-fold (6.402 U/mg). The 108-kDa rVrSBEⅡwas also detected by anti-6x His-tag mouse monoclonal antibodies in Western blot. In summary, VrsbeⅡ has been expressed into a biologically functional protein in E. coli system and expected to further improve its enzymatic properties by genetic engineering for application in food use.
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Mao-Jung, Chen, and 陳茂榮. "The expression of starch phosphorylase and proteomic analysis of mungbean (vigna radiata L.) during developing stage." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/67093605494802195473.
Full textAbstract:
碩士<br>中國醫藥大學<br>營養研究所<br>93<br>The objectives of this study were to use proteomic approach to identify and analyze the expression of starch phosphorylase (SP) in mungbean (Vigna radiata L. cv KPS1) and proteins that might be related to SP. Besides, the proteomes in mungbean of different developing stages were analyzed in order to investigate what roles they might play in starch biosynthesis. Mungbeans from four stages DAF 11, 14, 18 and 21 (DAF, day after flowering) were collected and extracted as experimental materials. When MASS was cooperated with Western blotting using 55 kDa-SP antibody to detect SP related proteins, two forms of SP, L-SP and H-SP, were identified in the developing stages. A trend that L-SP expression decreased as mungbean grew was found. Some enzymes involved in starch biosynthesis were also identified such as sucrose synthase, phosphoenolpyruvate carboxylase and glucose-6-phosphate isomerase. MASS was also cooperated with in situ activity staining of branching enzyme (BE). The protein bands that synthesized amylopectin not only identified SP and sucrose synthase, but other metabolic enzymes (enolase, phosphoglycerate kinase, fructose-bisphosphate aldolase and malate dehydrogenase). However, no any known BE species has yet matched in the searched database. 2-D electrophoresis was cooperated with MASS to analyze mungbean proteomes in different developing stages that their 2-D mappings and the proteome database of DAF 18 mungbean were established. There were 61 protein spots identified, within which 12 protein spots were related with starch synthesis. The mungbean proteome database will be used for protein searching, matching and identification in the future.
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Tu, Shuh Long, and 涂世隆. "Purification and charcacterization of plasma membrane inorganic H+-pyrophosphatase from mung bean (Vigna radiata L.) seedlings." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/77595878116686078320.
Full textAbstract:
碩士<br>國立清華大學<br>輻射生物研究所<br>84<br>Membrane-bound inorganic pyrophosphatase (PPase), which couples
PPi hydrolysis with proton pumping, has been found on membranes
of the tonoplast, chloroplast, mitochondria, and some species
of bacteria. Here, we further demonstrated the existence of a
H+-pumping PPase from mung bean plasma membrane. Enzyme
activity of PPase was observed on the plasma membrane and
possessed an optimum alkaline pH at 8.0-8.5. PPi-dependent
proton translocation was concomitantly found on highly purified
plasma membrane vesicles. A successful protocol including
plasma membrane preparation, detergent solubilization, gel
filtration, and anion exchange chromatographies was established
to purify the enzyme. Analysis of size exclusion gel filtration
chromatography and SDS-PAGE revealed that plasma membrane H+-
PPase was probably in a heterodimer form consisting of two
subunits of 65 and 67 kDa. The PPase activity was fluoride-
sensitive, but could be stimulated by K+ and phospholipid using
Mg2+ as a cofactor at Mg2+ / PPi ratio of 2 : 1. Taken
together, we believed that plasma membrane H+-PPase was a novel
new type of membrane-bound alkaline inorganic pyrophosphatase.
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Lu, Yi-Shan, and 盧奕珊. "The proteomic study of the UV-C radiation and gamma-ray irradiation effect for Vigna radiata." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/98838049577054206736.
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碩士<br>高雄醫學大學<br>醫學影像暨放射科學系碩士班<br>101<br>UV-C is high-energy non-ionized radiation in solar. Plant can not avoid UV-C radiation in environment. Exposure to gamma ray irradiation is a method to prevent bacterial fungi contamination for food sterilization. In this study, the Vigna radiata was expressed to UV-C radiation, applied exogenous Vitamin C and gamma-ray irradiation. The concentration of malondialdehyde, flavonoids and the DPPH radical scavenging activity after treatment on Vigna radiata were measured by ELISA method. To enhance our understanding of Vigna radiata proteome, the proteins were analyzed using proteomic approaches followed by peptide fragmentation patterning. In the experimental results, exogenous Vitamin C induce lateral root development and the malondialdehyde was down-regulated, flavonoids and the DPPH radical scavenging activity were up-regulated after applied exogenous Vitamin C and gamma ray irradiation. The DPPH radical scavenging activity was done-regulated after UV-C radiation. In this study, three proteins related with non-ionized radiation damage, five proteins related with ionized radiation damage and five proteins related with applied exogenous Vitamin C affect were identified. Those proteins may affect the plant growth, development and metabolism.
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Chang, Kai-Chieh, and 張凱傑. "Transcriptomic Analysis of Photosynthesis-related Genes in Non-leaf Green Tissues of Mung Bean (Vigna radiata)." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/y8j4gy.
Full textAbstract:
碩士<br>國立臺灣師範大學<br>生命科學系<br>107<br>Leaves are the major organ where higher plants perform photosynthesis. Other green tissues, known as non-leaf green tissue (NLGT), also contain photosynthetic activities. Plants rely on leaf photosynthesis to provide utilization of the entire plant, while photosynthesis of NLGT primarily provides the tissue's own needs. Our previous data demonstrated that the chlorophyll content and chlorophyll fluorescence Fv / Fm were changed in the different development stages of mung bean seeds, indicating that seeds may have photosynthesis ability. Therefore, this research aims to understand the expression differences of photosynthesis-related gene between leaf and NLGT. We first performed next-generation sequencing using mung bean seeds and leaves to represent NLGT and control tissue, respectively. The results showed that the expression of photosynthesis- and chlorophyll-related genes were down regulated, whereas that of the pyruvate metabolic genes were up regulated in the seed coat (testa) and cotyledon. Later on, the differences in gene expression between seed cotyledons and germinated cotyledons were compared. The results revealed that the photosynthesis-related genes in the cotyledons after germination had higher expression level. In summary, this study provides transcriptome information regarding photosynthesis in different tissues of mung bean leaves and seeds, which can be used in the future to adjust the photosynthetic efficiency of NLGT.
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Liu, Kun-Hsiang, and 劉坤湘. "Cloning and Characterization of Lipid Transfer Protein I Genes in Rice (Oryza sativa) and Mungbean (Vigna radiata)." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/87263330793010233372.
Full textAbstract:
博士<br>國立清華大學<br>生命科學系<br>91<br>ABSTRACT
To explore the changes in response to environmental stresses, we isolated a lipid transfer protein (LTP) gene in rice (Oryza sativa) suspension cells in the presence or absence of sucrose by using mRNA differential display. LTPs are small and basic proteins, which consist of eight highly conserved cysteines forming four disulfide bonds. Two main families of LTPs, LTPI and LTPII, are identified with molecular mass of 9 kDa and 7 kDa. LTPs exist in various plants, and the expression of plant LTPs shows temporal and spatial patterns. We found this rice ltp (Osltp) mRNA expressed in developing and fresh seeds, as well as roots in mature plants; and the level of Osltp mRNA increased under water stress, such as high salt, dehydration, low temperature, and abscisic acid (ABA) treatments. Furthermore, we isolated two novel mungbean (Vigna radiata) ltp genes, Vrltp1 and Vrltp2, by screening cDNA library. Both Vrltp1 and Vrltp2 mRNAs expressed in floral buds and only Vrltp1 mRNA expressed in immature seeds. In the vegetative tissues, the Vrltp1 and Vrltp2 mRNAs display their shoot specificity. The levels of Vrltp1 and Vrltp2 mRNAs also increase in response to salt, dehydration and ABA treatments. We assume that these ltp genes participate in the protection from damage under water stress, and the regulatory mechanisms are needed to be elucidated.
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GUO, SHUN-YU, and 郭順宇. "An essential arginyl residue in the tonoplast H+ -pyrophosphatase from etiolated mung bean seedings (Vigna radiata L.)." Thesis, 1989. http://ndltd.ncl.edu.tw/handle/28238137788870352086.
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Bollatti, María Jimena, la Fuente Federico Nicolás De, Juan Carlos Salich, and Roberto Oscar Tealdi. "Efecto de la fecha de siembra en la productividad de poroto mung (Vigna radiata (L.) R Wilczek)." Bachelor's thesis, 2017. http://hdl.handle.net/11086/6017.
Full textAbstract:
Trabajo Final Integrador (Área de Consolidación Sistemas Agrícolas de Producción Extensivos) -- UNC- Facultad de Ciencias Agropecuarias, 2017<br>El objetivo de este trabajo fue analizar tres factores: fecha de siembra
(dos niveles), distancia entre hileras (dos niveles) e inoculación (dos niveles)
sobre Poroto Mung (Vigna radiata L.Wilczek) conducidos en secano durante
la campaña 2016/17 en el Área Experimental del Campo Escuela de la
Facultad de Ciencias Agropecuarias UNC (31°28’43’’S 64°00’28’’ W). El
diseño utilizado fue en bloques completos al azar con tres repeticiones y dos
distancias entre hileras, con el agregado de inoculante en parcelas
designadas y lotes testigos sin inocular en el resto, sembradas en dos fechas
distintas, separadas por 60 días aproximadamente. Se registró el
rendimiento (kg/ha), el Nº semillas/m2, el peso de las semillas (g), la
biomasa aérea (g), la humedad de los granos cosechados y el índice de
cosecha. No se encontraron diferencias significativas, desde el punto de
vista estadístico, para ambas fechas de siembra, agregado de inoculante
específico para el cultivo y espaciamiento entre hileras (exceptuando la FS1
a 0,26 m donde si se encontró diferencia). Sin embargo, estadísticamente
hablando, a nivel de medias los tratamientos con fecha de siembra tardía,
menor espaciamiento entre hileras (0.26 m) y con inoculante fueron
superiores, pero esas diferencias no fueron estadísticamente significativas.
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Tsai, Chia Ling, and 蔡佳玲. "The Effects of Linker Length and Disulfide-bond Constraint on Circularly Permuted Vigna radiata Plant Defensin 1." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/57982571659202654455.
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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>104<br>Circular permutation (CP) is a protein-engineering technique to improve the characteristics of protein. In contrast with traditional mutagenesis, CP requires a linker to connect the native termini and creates new termini to result in the rearrangement of protein sequence. Vigna radiata plant defensin 1 (VrD1) is a cysteine-rich protein containing four disulfide bonds, and has a disulfide bond (C3-C46) bridging the native termini. We applied CP to VrD1 for exploring the influence of linker length and the effect of CP on cysteine-rich protein. The characteristics of structure and function in CP-VrD1 were analyzed through circular dichroism, intrinsic fluorescence, and α-amylase inhibition assay. We found that linkers with length less than the distance between original termini resulted in structural change and losing functions. Therefore, we speculated the poor folding of CP-VrD1 with shorter linker was due to the simultaneous existence of shorter linker and the terminal disulfide bond (C3-C46). Site-directed mutagenesis was used to remove terminal disulfide-bond in CP-VrD1 with linkers of different length, and to further elucidate the influence of linker length and disulfide-bond. Our results showed that terminal disulfide-bond removed CP-VrD1 still has poor structural folding and less thermal stability, but the function was not changed. We concluded that poor folding of CP-VrD1 was caused by the constraint of linker with shorter length rather than the existence of terminal disulfide bond (C3-C46).
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Chen, Hsiao-Ting, and 陳筱婷. "Expression of recombinant mungbean (Vigna radiata L.) starch branching enzyme I (pET-28a-VrsbeI) in Escherichia coli." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/66269836594024723546.
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碩士<br>國立臺灣海洋大學<br>食品科學系<br>98<br>Starch branching enzyme (SBE, EC 2.4.1.18) is one of the enzymes vital for amylopectin synthesis. The sequences of the previously obtained full-length cDNA of mungbean (Vigna radiata, cv. Tainan no. 5) sbeI (VrsbeI) and sbeII (VrsbeII’) were cloned. This study contains two parts: recombinant protein rVrSBEI expression analysis and chimeric gene design. First, pET-28a-VrsbeI plasmid DNA was transformed into BL21 (DE3) host cell and to find the optimal induction condition. The conditions for rVrSBEI induction were evaluated when the cells were grown at 37℃ until OD600 to 0.6 and then induced with 0.4 mM IPTG for 15 hrs; it showed seemingly a 117 kDa protein was induced. This recombinant protein was separated in and isolated from sodium dodecyl sulfate (SDS)-
polyacrylamide gels for mass spectrometric identification. The induced protein band, however, is identified to be LacZ protein. The sequence feactures of the functional domain of VrsbeI and VrsbeII’ were used to design chimeric genes, then using specific primers that amplified two cDNA fragments: VrsbeI 1643 bp (VrsbeI 5’-1643) and VrsbeII 360 bp (VrsbeII 3’-360). In the future, we can ligate the two fragments into a chimeric sbe cDNA (cVrsbeI-II). Next, we can transform, express and analyze the chimeric enzyme activity to further use the enzyme in the modification of starch molecules.
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Tsai, Ping-Hsing. "Identification of critical amino-acid residues in Vigna radiata plant defensin 1 involved in inhibiting Tenebrio molitor -amylase." 2006. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0016-1303200709293821.
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Tsai, Ping-Hsing, та 蔡秉興. "Identification of critical amino-acid residues in Vigna radiata plant defensin 1 involved in inhibiting Tenebrio molitor α-amylase". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/99284359689655527676.
Full textAbstract:
碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>94<br>Vigna radiata defensin 1 (VrD1) is a small, basic and cysteine-rich peptide of 46 amino acids. In former study, VrD1 was reported to exhibit insecticidal activity, and three dimensional structure of VrD1 have been determined by nuclear magnetic resonance (NMR) spectroscopy. However, the insecticidal mechanism of VrD1 is still indistinct. Our preliminary data showed that VrD1, which was purified from mung bean, inhibited Tenebrio molitor α-amylase. To elucidate the α-amylase inhibition mechanism of VrD1, recombinant VrD1 was constructed, expressed and purified from Escherichia coli. According to amino acid sequence analysis and protein structure comparison, specific residues involved in α-amylase inhibition were identified by site-directed mutagenesis. Eleven mutants were totally obtained and analyzed by circular dichroism (CD) for secondary structure and α-amylase activity assay for the inhibition function. The CD spectra showed that all recombinant VrD1 proteins have similar secondary structures. The results of α-amylase inhibition assay show that three mutants, K6A, R26E and R38A, significantly decrease in a-amylase inhibition. These three residues may play important roles in inhibitory function in VrD1.
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Chang, Jia-Wei, and 張家瑋. "Characterization and expression of mungbean (vigna radiata L.) starch branching enzyme I (vrsbe I) cDNA in E. coli system." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/68631256618981405316.
Full textAbstract:
碩士<br>國立臺灣海洋大學<br>食品科學系<br>95<br>Starch branching enzyme（SBE, EC 2.4.1.18）is one of the enzymes vital for amylopectin synthesis. The sequence of the previously obtained full-length cDNA of mungbean (Vigna radiate, cv. Tainan no. 5) SBEⅠ (named VrsbeⅠ) was confirmed and cloned. Its 3-D structures and functional features were predicted in silico with the only template, E. coli glycogen synthase (GS), 1m7x, by Swiss Model and REMUS. There is 27.4% amino acid sequence of VrSBEⅠ homologous to E. coli GS, in which 34.3% 3D structure of VrSBEⅠ was predicted. Six of the 8 conserved catalytic residues within the (α/β)8 domain of the α-amylase family in VrSBEⅠ were located in the structure. When using REMUS to compare amino acid sequences between two VrSBE isoforms, there were found to be potential epitope regions which are able to be bound specifically by antibodies. After confirming codon correctness by sequencing, VrsbeⅠ was cloned into pET-30 EK/LIC expression vector. The pET-30 EK/LIC-VrsbeI was expressed in BL21（DE3）pLysS cells in standard LB broth and the protein was induced by IPTG. The recombinant enzyme, rVrSBEⅠ, had His-tag and S-tag at the N terminal with an estimated molecular mass of 89 kDa. When Supplied with an extra 1% Glucose as carbon source during induction, it was able to decrease basal protein expression in the E. coli host. The crude cell extract possessing branching enzyme catalytic activities that decrease the A660 absorbance of amylose-iodine complex indicated that rVrSBEⅠ protein would be expressed as an active form. The crude extract was purified by HisTrapTM affinity chromatography. The activity of the purified rVrSBEI was also assayed by amylose branching assay. The decrease of the absorbance at A660 exhibited the specific activity of rVrSBEI was 314.6 U/mg and the purity has enriched 114-fold.
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Wang, Chih-Chiang, and 王志強. "The expression of Vigna radiata genes selected from temperature stress and the ubiquitin/26S proteasome pathway under low temperature." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/37966565431428413773.
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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>93<br>To identify cold-inducible genes in mungbean, we prepared a mungbean cDNA set containing independent cDNAs combined with 391 cDNAs isolated from 3 day old seedlings and 621 cDNA from subtraction for cold- and heat-treatment. This study focused on some of the candidate genes obtained from our preliminary microarray data. We aimed to study the mungbean genes involved in the ubiqitin/26S proteosome pathway from our cold-subtracted library, including the ubiquitin, ubiquitin conjugating enzyme, RING type ubiquitin ligase, 20S core protease, and 19S regulatory particle. We confirmed those genes using Northern blot analysis and finally identified the cold-induced ubiquitin conjugation genes. These results suggest that the ubiquitin conjugation genes have roles during cold stress through the turnover of protein(s) via the ubiquitin/26S proteasome pathway, but not heat.