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Dissertations / Theses on the topic 'Vigna radiate'

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Published: 3 June 2025

Last updated: 31 July 2025

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1

Kulkarni, A. A. "Role of enzymes/inhibitors from vigna radiata in host-pathogen interactions." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2007. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2539.

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2

Smith, Kristina J. "In vitro biosynthesis of pectic polysaccharides." Thesis, University of Stirling, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322091.

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3

Bordenave, Marianne. "Les pectine esterases de l'hypocotyle de vigna radiata : nature, fonctionnement et roles." Paris 6, 1995. http://www.theses.fr/1995PA066265.

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Des pectine methylesterases (pme, e. C. 3. 1. 1. 11), enzymes participant au controle du degre de methylation des pectines, donc de la cohesion de l'apoplasme, ont ete extraites de parois d'hypocotyles de vigna radiata. L'eluat salin des parois isolees a ete fractionne sur colonne de carboxy-methyl sepharose ; les pics d'activite esterase obtenus contenaient au moins trois isoformes de pme, ainsi qu'une acetyle-esterase (ae). Ces differentes activites ont ensuite ete purifiees par fplc. Les trois pme purifiees, pe#, pe# et pe#, presentaient respectivement des pi de 7,5, 9,3, et 9,8, des mr de

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4

Ahmed, Selina. "Studies on photosynthetic damage by waterlogging in mungbean (Vigna radiata (L.) Wilczak)." Kyoto University, 2002. http://hdl.handle.net/2433/149516.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第9800号<br>農博第1295号<br>新制||農||854(附属図書館)<br>学位論文||H14||N3722(農学部図書室)<br>UT51-2003-B340<br>京都大学大学院農学研究科地域環境科学専攻<br>(主査)教授櫻谷哲夫, 教授堀江武, 教授泉井桂<br>学位規則第4条第1項該当

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5

Parker, Beverly Jean 1944. "Water movement, structure and physiology in mung bean (Vigna radiata L.) leaves." Thesis, The University of Arizona, 1992. http://hdl.handle.net/10150/278118.

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Eight-day-old mung bean seedlings (Vigna radiata L.) grown in hydropondic medium were osmotically stressed by exposing roots to increasing concentrations of NaCl up to 4 bars. They were transferred after 16 to 18 hours to a similar solution containing tritiated water (THO). Periodic samples were taken of water transpiring from the leaves and of tissue water obtained from the same leaves, frozen and ground; specific radioactivity was determined by a scintillation counter. Proportional to increasing stress, the labelling of tissue water was increasingly delayed, the time for equilibration of the

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6

CHABANET, AGNES. "Peroxydases et laccase des parois de l'hypocotyle de vigna radiata : caracterisation et localisation." Paris 6, 1993. http://www.theses.fr/1993PA066331.

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Les peroxydases parietales peuvent participer a la rigidification des parois en catalysant la formation de ponts diphenols. Elles peuvent egalement intervenir, conjointement a des polyphenoloxydases (laccases), dans les parois en cours de lignification lors de la polymerisation des monolignols. Nous avons cherche a caracteriser et a localiser ces enzymes dans un materiel, l'hypocotyle de vigna radiata, qui presente un gradient de croissance bien defini. Apres avoir isole les proteines liees ioniquement aux parois, nous avons separe trois groupes de peroxydases cationiques egalement capables d'

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7

Noble, Thomas J. "Molecular characterisation and identification of Pseudomonas savastanoi pv. Phaseolicola, infecting mungbeans in Australia." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/205533/1/Thomas_Noble_Thesis.pdf.

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This research explores population genetics for the bacterial pathogen Pseudomonas savastanoi pv. phaseolicola, which causes halo blight disease in its host mungbeans (Vigna radiata). The pathogen and host populations were investigated at a board scale using field and glasshouse studies and in detail using molecular biology techniques including qPCR and next-generation sequencing. The study found both the bacterial pathogen and host (mungbean) to have highly conserved genetic backgrounds. This will make it easier for breeders to target critical resistance genes to prevent the infection of the h

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8

Amaral, Ana Lúcia da Silva. "Isolamento da vicilina do feijão mungo verde (Vigna radiata L.) e estudo de suas atividades hipocolesterolêmica e antimicrobiana /." Araraquara, 2016. http://hdl.handle.net/11449/144669.

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Orientador: Aureluce Demonte<br>Banca: Rosiane Gomes Silva Oliveira<br>Banca: Thais Milena de Souza Bezerra<br>Banca: Tais Maia Bauab<br>Banca: Juliana Cristina Bassan<br>Resumo: A homologia entre as características estruturais, sequenciais e funcionais da β- conglicinina da soja em relação a outras vicilinas de leguminosas e a ação de seus peptídeos bioativos nos estimulou a investigar a atividade da globulina vicilina (8S) do feijão mungo verde como composto funcional. Objetivo: isolar e caracterizar a globulina 8S do feijão mungo verde e verificar a ação hipocolesterolêmica e antimicrobiana

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9

Nogueira, Alexandre Verzani. "Manganese toxicity in mungbean [Vigna radiata (L.) Wilczek] and the effects of vesicular-arbuscular mycorrhiza on plant manganese tolerance." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384870.

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10

Allison, Patricia J. "Biosynthesis of pectic 1,4 #beta#-D-galactan in mung bean (Vigna radiata) and related studies on tomato (Lycopersicon esculentum)." Thesis, University of Stirling, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322046.

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11

Caldas, Marília Tiberi. "Qualidade fisiológica de sementes e brotos de feijão-mungo- verde." Universidade Federal de Viçosa, 2004. http://www.locus.ufv.br/handle/123456789/10564.

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Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2017-06-06T18:29:06Z No. of bitstreams: 1 texto completo.pdf: 1558482 bytes, checksum: 1a0c4385270f4da9acdf1b730e12eda4 (MD5)<br>Made available in DSpace on 2017-06-06T18:29:06Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1558482 bytes, checksum: 1a0c4385270f4da9acdf1b730e12eda4 (MD5) Previous issue date: 2004-03-11<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Os objetivos do presente trabalho foram avaliar a qualidade fisiológica das sementes de feijão-mungo-verde; a influência do etileno e

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Ray, Pushpanjali. "Search for novel actinomycetes from soil as potential biocontrol agent against fungal root pathogens of phaseolus vulgaris (L.) vigna radiata(L.)." Thesis, University of North Bengal, 2017. http://ir.nbu.ac.in/hdl.handle.net/123456789/2575.

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13

Ramirez, Tamariz Gilmar. "Influencia del crecimiento de la plántula sobre el contenido de folato del frejolito chino (Vigna radiata) en ausencia de luz." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2021. https://hdl.handle.net/20.500.12672/17053.

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El frejolito chino (Vigna radiata), legumbre considerada como “perla verde”, es poco consumido en Perú debido a limitados estudios realizados en este país acerca de sus nutrientes, compuestos bioactivos y efectos benéficos para la salud. El frejolito chino es consumido en Perú generalmente como plántula denominada comúnmente “germinado”, sin embargo, esta investigación destaca la diferencia entre germinado y plántula, además, establece un precedente en Perú acerca del contenido de folato en frejolito chino. La investigación determina la influencia del crecimiento de la plántula sobre e

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14

Na, Jom Kriskamol [Verfasser], Karl-Heinz [Akademischer Betreuer] Engel, and Wilfried [Akademischer Betreuer] Schwab. "Metabolite profiling of sprouting mung beans Vigna radiata / Kriskamol Na Jom. Gutachter: Karl-Heinz Engel ; Wilfried Schwab. Betreuer: Karl-Heinz Engel." München : Universitätsbibliothek der TU München, 2012. http://d-nb.info/1031514694/34.

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15

Bourgault, Maryse. "Legume production in semi-arid areas: comparative study of the physiology of drought tolerance in common bean («Phaseolus vulgaris L.») and mungbean «(Vigna radiata (L.) Wilczek)»." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40664.

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Context: Approximately one billion people live in semi-arid and arid regions, and of these about 40% live on less than a dollar a day. Legumes crops are an important component of sustainable agriculture, but they are often grown under intermittent or terminal droughts. Thus, improving drought tolerance in legumes has the potential to improve food security and sustainability of agricultural systems. Objective: This study compares the response of two legume crops, common bean (Phaseolus vulgaris L.) and mungbean (Vigna radiata (L.) Wilczek), to water stress conditions under field and contro

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NEVES, Ariana Morais. "Adubação verde com feijão mungo na viabilidade agroeconômica da hortelã." Universidade Federal de Campina Grande, 2017. http://dspace.sti.ufcg.edu.br:8080/jspui/handle/riufcg/2175.

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Submitted by Auxiliadora Costa (aucydj@gmail.com) on 2018-11-12T21:29:59Z No. of bitstreams: 1 ARIANA MORAIS NEVES - DISSERTAÇÃO PPGSA ACADÊMICO 2018.pdf: 1603264 bytes, checksum: 3b3b0373153433c9fd4557472da2f96c (MD5)<br>Made available in DSpace on 2018-11-12T21:30:00Z (GMT). No. of bitstreams: 1 ARIANA MORAIS NEVES - DISSERTAÇÃO PPGSA ACADÊMICO 2018.pdf: 1603264 bytes, checksum: 3b3b0373153433c9fd4557472da2f96c (MD5) Previous issue date: 2017-12-08<br>O uso de adubos verdes constitui em alternativa viável para ser utilizado pelos agricultores que produzem em sistema orgânico, dado os grand

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Miyagi, Mikiko. "Exploitation of bacterial artificial chromosome (BAC) libraries to enhance the efficiency of genome mapping." Thesis, Queensland University of Technology, 2002. https://eprints.qut.edu.au/37140/6/37140_Digitised%20Thesis.pdf.

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18

NEVES, Ana Paula Morais. "Viabilidade agroeconômica da alface (Lactuca sativa L.)." Universidade Federal de Campina Grande, 2016. http://dspace.sti.ufcg.edu.br:8080/jspui/handle/riufcg/818.

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Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-05-27T13:22:22Z No. of bitstreams: 1 ANA PAULA MORAIS NEVES - DISSERTAÇÃO PPGSA PROFISSIONAL 2017..pdf: 735461 bytes, checksum: c320ee1307d508a5d5811325882d133c (MD5)<br>Made available in DSpace on 2018-05-27T13:22:22Z (GMT). No. of bitstreams: 1 ANA PAULA MORAIS NEVES - DISSERTAÇÃO PPGSA PROFISSIONAL 2017..pdf: 735461 bytes, checksum: c320ee1307d508a5d5811325882d133c (MD5) Previous issue date: 2016-11-29<br>Uso de leguminosas como adubo verde é uma prática bastante consolidada, pelo fato de estar adicionando ao solo materia

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19

WU, JIA-JIN, and 吳珈瑾. "Functionality of mung bean (Vigna radiate L.) hydrolysates fermented by probiotic." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/55890434315175129025.

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碩士<br>國立高雄海洋科技大學<br>水產食品科學研究所<br>104<br>This study aimed to improve the functionality of mung bean, sample was hydrolyzed by cellulase and amylase, and further fermented by Lactobacillus johnsonii BCRC 17010 or Lactobacillus plantarum subsp. BCRC 10069. After 2 hr hydrolysis at 50oC by the combination of 1% cellulase and 1% α-amylase and further 24 hr fermentation with Lactobacillus johnsonii BCRC 17010 or Lactobacillus plantarum subsp. BCRC 10069 at 37oC, the LAB counts increased to 9.36 and 8.59 log CFU/mL, and the pH declined to 3.77 and 4.11, respectively. Scanning electron microscope (SEM

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20

Lin, Chia-Hui, and 林嘉慧. "Characterization of a Novel Y2K-type Dehydrin VrDhn1 from Mungbean (Vigna radiate L.)." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/41134026396992997187.

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博士<br>國立清華大學<br>生物資訊與結構生物研究所<br>101<br>A novel dehydrin gene (VrDhn1) was isolated from an embryo cDNA library of Vigna radiata (L.) Wilczek (mungbean) variety VC1973A. The intronless VrDhn1 gene encodes a protein belonging to the Y2K-type dehydrin family. VrDhn1 protein accumulated in embryos and cotyledons during seed maturation and disappeared 2 days after seed imbibition (DAI). The expression of VrDhn1 mRNA and accumulation of VrDhn1 protein were at high levels in mature seeds, but neither mRNA nor protein was detected in mungbean vegetative tissues under normal growth conditions. VrDh

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21

Yu, Yueh-hsiang, and 游約翔. "Substrate specificities of Hsc70s of Vigna radiata." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/09698364834473851860.

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碩士<br>國立清華大學<br>生命科學系<br>90<br>The 70 kilodalton heat shock cognate protein (Hsc70) is a constitutively expressed member of the molecular chaperones which serve to prevent protein misfolding and aggregation in the crowded environment of the cell. Hsc70 cooperates with cochaperone Hsp40, and functions by binding and releasing the exposed extended polypeptide of nascent and nonnative proteins, in the ATP-dependent manner. Binding specificities of Hsc70s to their substrates are quite different among bacteria, yeast, vertebrate and plant. To investigate the interactions and specificity between

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22

Thuan, Nguyen Dat. "Expression and inheritance of traits in wild mungbean (Vigna radiata ssp. sublobata) x cultivated mungbean (v. Radiata ssp. radiata) hybrids." Thesis, 2011. https://researchonline.jcu.edu.au/31508/1/31508_Thuan_2011_thesis.pdf.

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Mungbean (Vigna radiata (L.) Wilczek ssp. radiata) is an economically important crop in Asian countries where breeding research is being undertaken to improve varietal adaptation, yield and seed quality. The wild mungbean (ssp. sublobata) is a potentially useful adjunct to breeding, as the wild accessions possess traits that confer adaptation in their natural environments. The wild accession ACC 87 collected near Townsville has been identified as being perennial, a potentially useful trait for forage crop improvement. Accession ACC 1, from Mackay is very late flowering and was reported to poss

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Peng, Shu-Kai, and 彭書愷. "Cloning and characterization of a mungbean (Vigna radiata L.) starch phosphorylase cDNA." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/05958385857945258069.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>95<br>Starch is the major carbohydrate reserve in higher plants. In the starch biosynthetic pathway, starch phosphorylase (EC 2.4.1.1, SP) catalyzes a reversible reaction between synthesis and degradation of starch with bidirectional activities. Mungbean (Vigna radiata L.) starch is the most special that amylose content was 15-30% higher than normal level, which implied that SP plays an important role in amylose synthesis. Previous studies have identified the tryptic fragments of mungbean 105-kDa SP by MALDI-TOF and conducted immunological analysis. The objective

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Chen, Yu-Ting, and 陳郁婷. "Characterization and product of mungbean (Vigna radiata L.) starch phosphorylase recombinant protein." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/58906698204988611440.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>100<br>Starch phosphorylase (SP; EC 2.4.1.1) is one of the enzymes for starch metabolism. The aims of this study were to optimize the expression of a potential recombinant mungbean (Vigna radiata) sp clone (pMAL-c2X-Vrsp-modify) in the E. coli system, and to investigate the enzymatic characteristics of the recombinant SP (rVrSP) in the synthesis direction. Items include stable product formation condition, enzyme kinetics, and analysis of the enzyme products. Evaluation of the optimal expression condition for rVrSP showed that the cells grown at 37℃ until A600nm 0.6,

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25

Chia, Hsu Hung, та 許宏嘉. "Circular permutation on cysteine- stabilized αβ motif of Vigna radiata plant defensin 1". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/85t3v2.

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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>103<br>Mung bean (Vigna radiata) plant defensin 1 (VrD1) is the first reported plant defensin, which exhibits insecticidal activity against Callosobruchus chinensis (bruchid). VrD1 is a 46-residue basic peptide containing a cysteine-stabilized αβ (CSαβ) motif with four disulfide-bonds to stable its structure. Three dimensional structure determined by nuclear magnetic resonance (NMR) spectroscopy and alanine substitutions of noncysteine residues in VrD1 were done in our lab. Our previous studies showed that the loop L3 and the 310 helix played important roles in

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Shih, Yun-Chi, and 石韻琦. "Cloning and characterization of starch branching enzyme cDNA in mungbean (Vigna radiata L.)." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/70381090844490007521.

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碩士<br>中國醫藥大學<br>營養研究所<br>92<br>Abstract Starch branching enzyme (SBE, EC 2.4.1.18) is one of the enzymes in the starch biosynthetic pathway. It catalyzes the formation of branches, which plays a vital role in amylopectin synthesis. The objectives of this thesis are to clone full-length cDNA of SBE and analyze its structure and characteristics. Based on database search, the conserved regions from published SBE genes were obtained and used to design gene-specific primers for cloning. First, the mRNA from developing mungbean (Vigna radiata, cv. Tainan no.5) was extracted and used as te

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呂曉鈺. "Cloning and characterization of mungbean (Vigna radiata L.) granule-bound starch synthase cDNA." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/81563205899504005617.

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Chen, Mei-Yun, and 陳美雲. "Purification and Characterization of an Asparaginyl Endopeptidase from Mung bean（Vigna radiata）seedlings." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/32715798981981951815.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>94<br>Abstract To understand the physiological function of VrPE-1, a putative asparaginyl endopeptidase, in the cotyledons of germinated mung beans (Vigana radiate), a polyclonal antibody against VrPE-1 and an enzymatic activity of asparaginyl peptidase with Z-Ala-Ala-Asn-MCA as a substrate were utilized for the analysis of the expression and the purification of VrPE-1 from germinated mung beans. By Western blot analysis, VrPE-1 occurs in cotyledons, hypocotyls and roots, but not in leaves, of germinated mung beans on 5 DAI (days-after-imbibition), and its expressio

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Chan, Sam-I., and 陳心儀. "Characterization of Mungbean (Vigna radiata L.) Starch Branching Enzyme II in E. coli host." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/11662093372678656379.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>97<br>Starch branching enzyme (SBE, EC 2.4.1.18) is one of the enzymes in the starch biosynthetic pathway and it plays a vital role in the formation of amylopectin, the main component of the starch molecule. The chemical fine structure changed by SBE would affect the physical property and function of starch. The objective of the thesis was to optimize the expression of a potential recombinant SBEII clone and characterize the active recombinant enzyme to be used in modifying the starch molecular structure. The constructed pET21b-VrSBEⅡwas first transformed into BL21 (

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Chang, Wei-Chi, and 張瑋齊. "Construction of a mungbean (Vigna radiata L.) starch phosphorylase cDNA in E. coli system." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/20361438425510788682.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>97<br>Starch phosphorylase ( SP, EC 2.4.1.1 ) is commonly found in plants and is one of the vital enzymes in the starch metabolic pathway. It catalyzes a reversible reaction between synthesis and degradation of starch with bidirectional activities. Previous studies have identified the fragments of mungbean 105-kDa SP by MALDI-TOF, conducted immunological analysis and amplified the full-length cDNA fragment of mungbean (Vigna radiata L.) SP (named VrSP). The objective of this study was to clone the sequence into an expression vector and further express into a biologic

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Ku, BI-Jan, and 古璧甄. "Study on Cultivation of Mungbean (Vigna radiata (L.) Wilczek) Sprout with High Phytochemical Content." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/95033178855258254465.

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碩士<br>國立臺灣大學<br>園藝學研究所<br>103<br>Two isogenic lines of mungbean, bruchid-resistant VC6089A and bruchid-susceptible VC1973A, and Sunright mungbean (Sunright Foods Co.) were used as experimental material to study the optimal environmental condition of cultivating mungbean sprout with high phytochemicals contents in the dark. Mungbean seeds were soaked at 20˚C, 26˚C and 32˚C for 0、4、8 and 12 h. Seed dry weight decreased as the soaking time extending to 12 h. Among 4 planting densities, 7 g·cm-2 treatment showed the highest harvest index, longer hypocotyl length and higher total flavonoids content

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Wang, Jyun-Jheng, and 王鈞正. "Expression of mungbean (Vigna radiata L.) starch phosphorylase recombinant protein in E. coli system." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/70346398588369100221.

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Chen, Shu-Ling, and 陳淑玲. "Chilling Stress Effects the Plastid Development and Gene Expression in mungbean （Vigna radiata L. ）seedlings." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/05510974194634430520.

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博士<br>國立臺灣大學<br>植物學研究所<br>87<br>Mungbean ( Vigna radiata L. cv Tainan no. 5 ) is a chilling-sensitive plant. The growth of etiolated mungbean seedlings was very slowly at 10 oC in the light. The leaves continued to grow and finally expranded as those grown at 28oC in the light, but they did not turn to green. The development of plastids into chloroplasts in the etiolated leaves was retarded under chilling in the light condition when leaf ultrastructure was examined under T. E. M.. The synthesis of chlorophyll in the leaves were also retarded by 10 oC-chilling treatment. By SDS-PAGE and 2D-PAGE

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Chen, Yung-Che, and 陳詠哲. "Purification and Functional Analysis of the Bruchid Resistant Protein VrCRP from Vigna radiata VC 6089A." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/08554565764190812266.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>89<br>Abstract A cDNA named VrCRP (cysteine-rich protein of Vigna radiata) was previously isolated in our laboratory from an insect resistant nearly isogenic line of mung bean Vigna radiata VC6089A (Abbreviated as VC6089A) versus a susceptible isogenic line Vigna radiata VC1973A using suppressive substractive hybridization. The recombinant protein VrCRPDsp (Dsp represents signal peptide truncated) was shown to be highly toxic to one of the major bruchids of mung bean seeds Callosobruchus chinensis. In order to study the structure, properties and biological

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Chiu, Shu Jun, and 邱淑君. "Studies on gene structure of mung bean (Vigna radiata L.) Vacuolar H+-ATPase A subunit." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/37437452062442227865.

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碩士<br>國立清華大學<br>輻射生物研究所<br>84<br>Vacuoles of higher plant cells contain two primary electrogenic proton pumps, a vacuolar H+-ATPase (V-ATPase) and a H+- translocating inorganic pyrophosphatase (V-PPase), for the regulation of cell turgor, cytoplasmic homeostasis, and the storage of metabolites. In this work, the structure of the mung bean V-ATPase A subunit gene was investigated. In order to clone the cDNA, primers were synthesized according to the conserved sequences of cotton and carrot V

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Chen, Li-Ru, and 陳麗如. "Comparative gene expression profiles of mungbean, Vigna radiata (L.) Wilczek, seedlings in response to cold." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/32766895851325010213.

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博士<br>國立清華大學<br>生命科學系<br>96<br>Mungbean (Vigna radiata (L.) Wilczek) is commonly used as human food. However, the genomic resources of this species available in databases are limited. This study has two sections, the expressed sequence tags (EST) related to early seedling development and chilling response are developed in the first part. Two mungbean varieties NM94 and VC1973A were obtained from the Asian Vegetable Research and Development Center-The World Vegetable Center (AVRDC). They differ in disease resistance and susceptibility to chilling temperatures. NM94 can maintain a better me

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Chung, Pei-Shan, та 鍾佩珊. "Expression of mungbean (Vigna radiata L.) starch branching enzyme ІІ recombinant protein in E. coli system". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/55793443226754783040.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>95<br>Starch branching enzyme (SBE, EC 2.4.1.18) is a vital enzyme for amylopectin synthesis in the starch biosynthetic pathway. The aim of this thesis was to subclone VrsbeII of mungbean (Vigna radiata L. cv Tainan no. 5) into expression vector and produce active enzymes in the E. coli system. First, VrsbeII was constructed into pET-30 EK/LIC vector and transformed into E. coli NovaBlue host cells, hopefully full length sequence of the open reading frame could be obtained. However, there was only approx. 350 bp fragment could be stably maintained in pET-30 EK/LIC sy

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Mao-Jung, Chen, and 陳茂榮. "The expression of starch phosphorylase and proteomic analysis of mungbean (vigna radiata L.) during developing stage." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/67093605494802195473.

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碩士<br>中國醫藥大學<br>營養研究所<br>93<br>The objectives of this study were to use proteomic approach to identify and analyze the expression of starch phosphorylase (SP) in mungbean (Vigna radiata L. cv KPS1) and proteins that might be related to SP. Besides, the proteomes in mungbean of different developing stages were analyzed in order to investigate what roles they might play in starch biosynthesis. Mungbeans from four stages DAF 11, 14, 18 and 21 (DAF, day after flowering) were collected and extracted as experimental materials. When MASS was cooperated with Western blotting using 55 kDa-SP antibody t

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Tu, Shuh Long, and 涂世隆. "Purification and charcacterization of plasma membrane inorganic H+-pyrophosphatase from mung bean (Vigna radiata L.) seedlings." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/77595878116686078320.

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碩士<br>國立清華大學<br>輻射生物研究所<br>84<br>Membrane-bound inorganic pyrophosphatase (PPase), which couples PPi hydrolysis with proton pumping, has been found on membranes of the tonoplast, chloroplast, mitochondria, and some species of bacteria. Here, we further demonstrated the existence of a H+-pumping PPase from mung bean plasma membrane. Enzyme activity of PPase was observed on the plasma membrane and possessed an optimum alkaline pH at 8.0-8.5. PPi-dependent proton translocation was concomitantl

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Lu, Yi-Shan, and 盧奕珊. "The proteomic study of the UV-C radiation and gamma-ray irradiation effect for Vigna radiata." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/98838049577054206736.

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碩士<br>高雄醫學大學<br>醫學影像暨放射科學系碩士班<br>101<br>UV-C is high-energy non-ionized radiation in solar. Plant can not avoid UV-C radiation in environment. Exposure to gamma ray irradiation is a method to prevent bacterial fungi contamination for food sterilization. In this study, the Vigna radiata was expressed to UV-C radiation, applied exogenous Vitamin C and gamma-ray irradiation. The concentration of malondialdehyde, flavonoids and the DPPH radical scavenging activity after treatment on Vigna radiata were measured by ELISA method. To enhance our understanding of Vigna radiata proteome, the proteins we

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Chang, Kai-Chieh, and 張凱傑. "Transcriptomic Analysis of Photosynthesis-related Genes in Non-leaf Green Tissues of Mung Bean (Vigna radiata)." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/y8j4gy.

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碩士<br>國立臺灣師範大學<br>生命科學系<br>107<br>Leaves are the major organ where higher plants perform photosynthesis. Other green tissues, known as non-leaf green tissue (NLGT), also contain photosynthetic activities. Plants rely on leaf photosynthesis to provide utilization of the entire plant, while photosynthesis of NLGT primarily provides the tissue's own needs. Our previous data demonstrated that the chlorophyll content and chlorophyll fluorescence Fv / Fm were changed in the different development stages of mung bean seeds, indicating that seeds may have photosynthesis ability. Therefore, this researc

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Liu, Kun-Hsiang, and 劉坤湘. "Cloning and Characterization of Lipid Transfer Protein I Genes in Rice (Oryza sativa) and Mungbean (Vigna radiata)." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/87263330793010233372.

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博士<br>國立清華大學<br>生命科學系<br>91<br>ABSTRACT To explore the changes in response to environmental stresses, we isolated a lipid transfer protein (LTP) gene in rice (Oryza sativa) suspension cells in the presence or absence of sucrose by using mRNA differential display. LTPs are small and basic proteins, which consist of eight highly conserved cysteines forming four disulfide bonds. Two main families of LTPs, LTPI and LTPII, are identified with molecular mass of 9 kDa and 7 kDa. LTPs exist in various plants, and the expression of plant LTPs shows temporal and spatial patterns. We foun

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GUO, SHUN-YU, and 郭順宇. "An essential arginyl residue in the tonoplast H+ -pyrophosphatase from etiolated mung bean seedings (Vigna radiata L.)." Thesis, 1989. http://ndltd.ncl.edu.tw/handle/28238137788870352086.

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Bollatti, María Jimena, la Fuente Federico Nicolás De, Juan Carlos Salich, and Roberto Oscar Tealdi. "Efecto de la fecha de siembra en la productividad de poroto mung (Vigna radiata (L.) R Wilczek)." Bachelor's thesis, 2017. http://hdl.handle.net/11086/6017.

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Trabajo Final Integrador (Área de Consolidación Sistemas Agrícolas de Producción Extensivos) -- UNC- Facultad de Ciencias Agropecuarias, 2017<br>El objetivo de este trabajo fue analizar tres factores: fecha de siembra (dos niveles), distancia entre hileras (dos niveles) e inoculación (dos niveles) sobre Poroto Mung (Vigna radiata L.Wilczek) conducidos en secano durante la campaña 2016/17 en el Área Experimental del Campo Escuela de la Facultad de Ciencias Agropecuarias UNC (31°28’43’’S 64°00’28’’ W). El diseño utilizado fue en bloques completos al azar con tres repeticiones y dos distanc

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Tsai, Chia Ling, and 蔡佳玲. "The Effects of Linker Length and Disulfide-bond Constraint on Circularly Permuted Vigna radiata Plant Defensin 1." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/57982571659202654455.

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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>104<br>Circular permutation (CP) is a protein-engineering technique to improve the characteristics of protein. In contrast with traditional mutagenesis, CP requires a linker to connect the native termini and creates new termini to result in the rearrangement of protein sequence. Vigna radiata plant defensin 1 (VrD1) is a cysteine-rich protein containing four disulfide bonds, and has a disulfide bond (C3-C46) bridging the native termini. We applied CP to VrD1 for exploring the influence of linker length and the effect of CP on cysteine-rich protein. The character

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Chen, Hsiao-Ting, and 陳筱婷. "Expression of recombinant mungbean (Vigna radiata L.) starch branching enzyme I (pET-28a-VrsbeI) in Escherichia coli." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/66269836594024723546.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>98<br>Starch branching enzyme (SBE, EC 2.4.1.18) is one of the enzymes vital for amylopectin synthesis. The sequences of the previously obtained full-length cDNA of mungbean (Vigna radiata, cv. Tainan no. 5) sbeI (VrsbeI) and sbeII (VrsbeII’) were cloned. This study contains two parts: recombinant protein rVrSBEI expression analysis and chimeric gene design. First, pET-28a-VrsbeI plasmid DNA was transformed into BL21 (DE3) host cell and to find the optimal induction condition. The conditions for rVrSBEI induction were evaluated when the cells were grown at 37℃ until

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Tsai, Ping-Hsing. "Identification of critical amino-acid residues in Vigna radiata plant defensin 1 involved in inhibiting Tenebrio molitor -amylase." 2006. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0016-1303200709293821.

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Tsai, Ping-Hsing, та 蔡秉興. "Identification of critical amino-acid residues in Vigna radiata plant defensin 1 involved in inhibiting Tenebrio molitor α-amylase". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/99284359689655527676.

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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>94<br>Vigna radiata defensin 1 (VrD1) is a small, basic and cysteine-rich peptide of 46 amino acids. In former study, VrD1 was reported to exhibit insecticidal activity, and three dimensional structure of VrD1 have been determined by nuclear magnetic resonance (NMR) spectroscopy. However, the insecticidal mechanism of VrD1 is still indistinct. Our preliminary data showed that VrD1, which was purified from mung bean, inhibited Tenebrio molitor α-amylase. To elucidate the α-amylase inhibition mechanism of VrD1, recombinant VrD1 was constructed, expressed and p

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Chang, Jia-Wei, and 張家瑋. "Characterization and expression of mungbean (vigna radiata L.) starch branching enzyme I (vrsbe I) cDNA in E. coli system." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/68631256618981405316.

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碩士<br>國立臺灣海洋大學<br>食品科學系<br>95<br>Starch branching enzyme（SBE, EC 2.4.1.18）is one of the enzymes vital for amylopectin synthesis. The sequence of the previously obtained full-length cDNA of mungbean (Vigna radiate, cv. Tainan no. 5) SBEⅠ (named VrsbeⅠ) was confirmed and cloned. Its 3-D structures and functional features were predicted in silico with the only template, E. coli glycogen synthase (GS), 1m7x, by Swiss Model and REMUS. There is 27.4% amino acid sequence of VrSBEⅠ homologous to E. coli GS, in which 34.3% 3D structure of VrSBEⅠ was predicted. Six of the 8 conserved catalytic residues

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Wang, Chih-Chiang, and 王志強. "The expression of Vigna radiata genes selected from temperature stress and the ubiquitin/26S proteasome pathway under low temperature." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/37966565431428413773.

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碩士<br>國立清華大學<br>生物資訊與結構生物研究所<br>93<br>To identify cold-inducible genes in mungbean, we prepared a mungbean cDNA set containing independent cDNAs combined with 391 cDNAs isolated from 3 day old seedlings and 621 cDNA from subtraction for cold- and heat-treatment. This study focused on some of the candidate genes obtained from our preliminary microarray data. We aimed to study the mungbean genes involved in the ubiqitin/26S proteosome pathway from our cold-subtracted library, including the ubiquitin, ubiquitin conjugating enzyme, RING type ubiquitin ligase, 20S core protease, and 19S regulator

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