Journal articles: 'VIM'

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SHORT, PATRICIA. "INJECTING VIM." Chemical & Engineering News 81, no. 21 (May 26, 2003): 7. http://dx.doi.org/10.1021/cen-v081n021.p007.

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Wang, Zheng, Alex Divanyan, Frances L. Jourd’heuil, Robert D. Goldman, Karen M. Ridge, David Jourd’heuil, and Reynold I. Lopez-Soler. "Vimentin expression is required for the development of EMT-related renal fibrosis following unilateral ureteral obstruction in mice." American Journal of Physiology-Renal Physiology 315, no. 4 (October 1, 2018): F769—F780. http://dx.doi.org/10.1152/ajprenal.00340.2017.

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Most renal transplants ultimately fail secondary to chronic allograft nephropathy (CAN). Vimentin (vim) is a member of the intermediate filament family of proteins and has been shown to be important in the development of CAN. One of the pathways leading to chronic renal fibrosis after transplant is thought to be epithelial to mesenchymal transition (EMT). Even though vim expression is one of the main steps of EMT, it is unknown whether vim expression is required for EMT leading to renal fibrosis and allograft loss. To this end, the role of vim in renal fibrosis was determined via unilateral ureteral obstruction (UUO) in vim knockout mice (129 svs6 vim −/−). Following UUO, kidneys were recovered and analyzed via Western blotting, immunofluorescence, and transcriptomics. Cultured human proximal renal tubular (HK-2) cells were subjected to lentiviral-driven inhibition of vim expression and then treated with transforming growth factor (TGF)-β to undergo EMT. Immunoblotting as well as wound healing assays were used to determine development of EMT. Western blotting analyses of mice undergoing UUO reveal increased levels of vim soon after UUO. As expected, interstitial collagen deposition increased in control mice following UUO but decreased in vim −/− kidneys. Immunofluorescence analyses also revealed altered localization of β-catenin in vim −/− mice undergoing UUO without significant changes in mRNA levels. However, RNA sequencing revealed a decrease in β-catenin-dependent genes in vim −/− kidneys. Finally, vim-silenced HK-2 cell lines undergoing EMT were shown to have decreased cellular migration during wound healing. We conclude that vim inhibition decreases fibrosis following UUO by possibly altering β-catenin localization and downstream signaling.

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Pournaras, Spyros, Alexandros Ikonomidis, Leonidas S. Tzouvelekis, Despoina Tokatlidou, Nicholas Spanakis, Antonios N. Maniatis, Nicholas J. Legakis, and Athanassios Tsakris. "VIM-12, a Novel Plasmid-Mediated Metallo-β-Lactamase from Klebsiella pneumoniae That Resembles a VIM-1/VIM-2 Hybrid." Antimicrobial Agents and Chemotherapy 49, no. 12 (December 2005): 5153–56. http://dx.doi.org/10.1128/aac.49.12.5153-5156.2005.

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ABSTRACT A transferable plasmid from Klebsiella pneumoniae carried a class 1 integron containing bla VIM-12, a novel bla VIM-type gene, flanked by two copies of aacA7. bla VIM-12 was clustered between bla VIM-1 and bla VIM-2 and differed from bla VIM-1 by 18 nucleotides that were all located at the 3′ end and matched the corresponding nucleotides in bla VIM-2. The bla VIM-12-associated 59-base element was identical to that described in bla VIM-2 alleles.

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David Mason. "Vim and Vinegar." Sewanee Review 118, no. 1 (2010): 104–13. http://dx.doi.org/10.1353/sew.0.0230.

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Al-Khaled, Kamel, and Ashwaq Hazaimeh. "Comparison Methods for Solving Non-Linear Sturm–Liouville Eigenvalues Problems." Symmetry 12, no. 7 (July 16, 2020): 1179. http://dx.doi.org/10.3390/sym12071179.

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In this paper, we present a comparative study between Sinc–Galerkin method and a modified version of the variational iteration method (VIM) to solve non-linear Sturm–Liouville eigenvalue problem. In the Sinc method, the problem under consideration was converted from a non-linear differential equation to a non-linear system of equations, that we were able to solve it via the use of some iterative techniques, like Newton’s method. The other method under consideration is the VIM, where the VIM has been modified through the use of the Laplace transform, and another effective modification has also been made to the VIM by replacing the non-linear term in the integral equation resulting from the use of the well-known VIM with the Adomian’s polynomials. In order to explain the advantages of each method over the other, several issues have been studied, including one that has an application in the field of spectral theory. The results in solutions to these problems, which were included in tables, showed that the improved VIM is better than the Sinc method, while the Sinc method addresses some advantages over the VIM when dealing with singular problems.

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Lassaux, Patricia, Daouda A. K. Traoré, Elodie Loisel, Adrien Favier, Jean-Denis Docquier, Jean Sébastien Sohier, Clémentine Laurent, et al. "Biochemical and Structural Characterization of the Subclass B1 Metallo-β-Lactamase VIM-4." Antimicrobial Agents and Chemotherapy 55, no. 3 (December 13, 2010): 1248–55. http://dx.doi.org/10.1128/aac.01486-09.

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ABSTRACTThe metallo-β-lactamase VIM-4, mainly found inPseudomonas aeruginosaorAcinetobacter baumannii, was produced inEscherichia coliand characterized by biochemical and X-ray techniques. A detailed kinetic study performed in the presence of Zn2+at concentrations ranging from 0.4 to 100 μM showed that VIM-4 exhibits a kinetic profile similar to the profiles of VIM-2 and VIM-1. However, VIM-4 is more active than VIM-1 against benzylpenicillin, cephalothin, nitrocefin, and imipenem and is less active than VIM-2 against ampicillin and meropenem. The crystal structure of the dizinc form of VIM-4 was solved at 1.9 Å. The sole difference between VIM-4 and VIM-1 is found at residue 228, which is Ser in VIM-1 and Arg in VIM-4. This substitution has a major impact on the VIM-4 catalytic efficiency compared to that of VIM-1. In contrast, the differences between VIM-2 and VIM-4 seem to be due to a different position of the flapping loop and two substitutions in loop 2. Study of the thermal stability and the activity of the holo- and apo-VIM-4 enzymes revealed that Zn2+ions have a pronounced stabilizing effect on the enzyme and are necessary for preserving the structure.

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Liu, Pengfei, Shengwei Zhang, Jingyi Ma, Dongning Jin, Yali Qin, and Mingzhou Chen. "Vimentin inhibits α-tubulin acetylation via enhancing α-TAT1 degradation to suppress the replication of human parainfluenza virus type 3." PLOS Pathogens 18, no. 9 (September 15, 2022): e1010856. http://dx.doi.org/10.1371/journal.ppat.1010856.

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We previously found that, among human parainfluenza virus type 3 (HPIV3) proteins, the interaction of nucleoprotein (N) and phosphoprotein (P) provides the minimal requirement for the formation of cytoplasmic inclusion bodies (IBs), which are sites of RNA synthesis, and that acetylated α-tubulin enhances IB fusion and viral replication. In this study, using immunoprecipitation and mass spectrometry assays, we determined that vimentin (VIM) specifically interacted with the N–P complex of HPIV3, and that the head domain of VIM was responsible for this interaction, contributing to the inhibition of IB fusion and viral replication. Furthermore, we found that VIM promoted the degradation of α-tubulin acetyltransferase 1 (α-TAT1), through its head region, thereby inhibiting the acetylation of α-tubulin, IB fusion, and viral replication. In addition, we identified a 20-amino-acid peptide derived from the head region of VIM that participated in the interaction with the N–P complex and inhibited viral replication. Our findings suggest that VIM inhibits the formation of HPIV3 IBs by downregulating α-tubulin acetylation via enhancing the degradation of α-TAT1. Our work sheds light on a new mechanism by which VIM suppresses HPIV3 replication.

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Juan, Carlos, Alejandro Beceiro, Olivia Gutiérrez, Sebastián Albertí, Margalida Garau, José L. Pérez, Germán Bou, and Antonio Oliver. "Characterization of the New Metallo-β-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain." Antimicrobial Agents and Chemotherapy 52, no. 10 (July 21, 2008): 3589–96. http://dx.doi.org/10.1128/aac.00465-08.

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ABSTRACT During a survey conducted to evaluate the incidence of class B carbapenemase (metallo-β-lactamase [MBL])-producing Pseudomonas aeruginosa strains from hospitals in Majorca, Spain, five clinical isolates showed a positive Etest MBL screening test result. In one of them, strain PA-SL2, the presence of a new bla VIM derivative (bla VIM-13) was detected by PCR amplification with bla VIM-1-specific primers followed by sequencing. The bla VIM-13-producing isolate showed resistance to all β-lactams (except aztreonam), gentamicin, tobramycin, and ciprofloxacin. VIM-13 exhibited 93% and 88% amino acid sequence identities with VIM-1 and VIM-2, respectively. bla VIM-13 was cloned in parallel with bla VIM-1, and the resistance profile conferred was analyzed both in Escherichia coli and in P. aeruginosa backgrounds. Compared to VIM-1, VIM-13 conferred slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime. VIM-13 and VIM-1 were purified in parallel as well, and their kinetic parameters were compared. The k cat/K m ratios for the antibiotics mentioned above were in good agreement with the MIC data. Furthermore, EDTA inhibited the activity of VIM-13 approximately 25 times less than it inhibited the activity of VIM-1. VIM-13 was harbored in a class 1 integron, along with a new variant (Ala108Thr) of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin. Finally, the VIM-13 integron was apparently located in the chromosome, since transformation and conjugation experiments consistently yielded negative results and the bla VIM-13 probe hybridized only with the genomic DNA.

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Samuelsen, Ørjan, Mariana Castanheira, Timothy R. Walsh, and James Spencer. "Kinetic Characterization of VIM-7, a Divergent Member of the VIM Metallo-β-Lactamase Family." Antimicrobial Agents and Chemotherapy 52, no. 8 (June 16, 2008): 2905–8. http://dx.doi.org/10.1128/aac.00166-08.

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ABSTRACT Purified recombinant VIM-7 possesses efficient penicillinase and carbapenemase activities comparable to those of VIM-2. Cephalosporinase activity was variable and generally lower than those of VIM-1 and VIM-2. A homology model suggests that the VIM-7 Tyr-218 Phe substitution may be responsible for the reduced catalytic efficiency against certain cephalosporins, including ceftazidime and cefepime.

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Papagiannitsis, Costas C., Simona Pollini, Filomena De Luca, Gian Maria Rossolini, Jean-Denis Docquier, and Jaroslav Hrabák. "Biochemical Characterization of VIM-39, a VIM-1-Like Metallo-β-Lactamase Variant from a Multidrug-Resistant Klebsiella pneumoniae Isolate from Greece." Antimicrobial Agents and Chemotherapy 59, no. 12 (September 14, 2015): 7811–14. http://dx.doi.org/10.1128/aac.01935-15.

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ABSTRACTVIM-39, a VIM-1-like metallo-β-lactamase variant (VIM-1 Thr33Ala His224Leu) was identified in a clinical isolate ofKlebsiella pneumoniaebelonging to sequence type 147. VIM-39 hydrolyzed ampicillin, cephalothin, and imipenem more efficiently than did VIM-1 and VIM-26 (a VIM-1 variant with the His224Leu substitution) because of higher turnover rates.

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Yan, Yu-Hang, Jian Chen, Zhen Zhan, Zhu-Jun Yu, Gen Li, Li Guo, Guo-Bo Li, Yong Wu, and Yongxiang Zheng. "Discovery of mercaptopropanamide-substituted aryl tetrazoles as new broad-spectrum metallo-β-lactamase inhibitors." RSC Advances 10, no. 52 (2020): 31377–84. http://dx.doi.org/10.1039/d0ra06405j.

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Compound 13a showed IC₅₀ values of 0.044 μM, 0.396 μM and 0.71 μM against VIM-2, NDM-1 and IMP-1 MBL, respectively. It binds to chelates via active site zinc ions and forms interactions with residues on the L1 and L3 loops of VIM-2.

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Makena, Anne, Azer Ö. Düzgün, Jürgen Brem, Michael A. McDonough, Anna M. Rydzik, Martine I. Abboud, Ayşegül Saral, Ayşegül Ç. Çiçek, Cemal Sandalli, and Christopher J. Schofield. "Comparison of Verona Integron-Borne Metallo-β-Lactamase (VIM) Variants Reveals Differences in Stability and Inhibition Profiles." Antimicrobial Agents and Chemotherapy 60, no. 3 (December 14, 2015): 1377–84. http://dx.doi.org/10.1128/aac.01768-15.

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Metallo-β-lactamases (MBLs) are of increasing clinical significance; the development of clinically useful MBL inhibitors is challenged by the rapid evolution of variant MBLs. The Verona integron-borne metallo-β-lactamase (VIM) enzymes are among the most widely distributed MBLs, with >40 VIM variants having been reported. We report on the crystallographic analysis of VIM-5 and comparison of biochemical and biophysical properties of VIM-1, VIM-2, VIM-4, VIM-5, and VIM-38. Recombinant VIM variants were produced and purified, and their secondary structure and thermal stabilities were investigated by circular dichroism analyses. Steady-state kinetic analyses with a representative panel of β-lactam substrates were carried out to compare the catalytic efficiencies of the VIM variants. Furthermore, a set of metalloenzyme inhibitors were screened to compare their effects on the different VIM variants. The results reveal only small variations in the kinetic parameters of the VIM variants but substantial differences in their thermal stabilities and inhibition profiles. Overall, these results support the proposal that protein stability may be a factor in MBL evolution and highlight the importance of screening MBL variants during inhibitor development programs.

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Ojobor, S. A., S. O. E. Inonoje, and E. J. Mamadu. "A proposed Method for the Solution of One-Dimensional Gas Dynamic Equation with Legendre and Chebyshev polynomials." Journal of Physics: Conference Series 2199, no. 1 (February 1, 2022): 012027. http://dx.doi.org/10.1088/1742-6596/2199/1/012027.

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Abstract We have considered a proposed method to efficiently handle the piecewise formulation in the generation of approximate solutions for gas dynamic equation via the Legendre and Chebychev basis functions. Two cases of the gas dynamic equation were considered for numerical illustrations aided by MAPLE 18 software. We did obtained some fascinating results. To be precise, applying the proposed method to the homogeneous gas dynamic via the Legendre polynomial, we attained a maximum error of order 10−4 as against that of VIM with a maximum error of order 10−3 at t = 0.0001. Also, the proposed method via Chebyshev polynomials at t = 0.0001 attained a maximum error of order 10−4 as against the VIM which has a maximum error of order 10−2. In like manner, applying the proposed method to the nonhomogeneous gas dynamic via the Legendre polynomials we attained a maximum error of order 10−4 at t = 0.0001 as against that of VIM, which does not show convergent. Also, proposed method via Chebyshev polynomials at t = 0.0001 attained a maximum error of order 10−4 as against the VIM which does converge at every grid points. Thus, it is obvious that the proposed method is a better convergent iterative scheme than the in as much as t decreases.

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Castanheira, Mariana, Jan M. Bell, John D. Turnidge, Dillip Mathai, and Ronald N. Jones. "Carbapenem Resistance among Pseudomonas aeruginosa Strains from India: Evidence for Nationwide Endemicity of Multiple Metallo-β-Lactamase Clones (VIM-2, -5, -6, and -11 and the Newly Characterized VIM-18)." Antimicrobial Agents and Chemotherapy 53, no. 3 (December 29, 2008): 1225–27. http://dx.doi.org/10.1128/aac.01011-08.

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ABSTRACT Among 57 metallo-β-lactamase (MβL)-producing Pseudomonas spp. detected in India during 2006, five bla VIM genes were found, including a newly characterized bla VIM gene. Pseudomonas aeruginosa strains were clustered in 33 ribotypes with clones found in multiple hospitals. Several types of bla VIM-2-carrying integrons were detected. The newly characterized variant VIM-18 showed a 4-amino-acid deletion compared to other VIM variants. In this study, we show that VIM-producing Pseudomonas spp. were highly prevalent in India with a great diversity of bla VIM types and MβL-carrying integrons.

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Zheng, Liang, and Zhengbing He. "A new car following model from the perspective of visual imaging." International Journal of Modern Physics C 26, no. 08 (May 3, 2015): 1550090. http://dx.doi.org/10.1142/s0129183115500904.

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The paper proposes a car following model from the perspective of visual imaging (VIM), where the visual imaging size of the preceding vehicle on a driver's retina is considered as the stimuli and determines the driving behaviors. NGSIM trajectory data are applied to calibrate and validate the VIM under two scenarios, i.e. following the car and following the truck, whose fitting performance outperforms that of visual angle car following model (VAM). Through linear stability analyses for VIM, it can be drawn that the asymmetry in traffic flow is preserved; the larger vehicle width, vehicle length and vehicle apparent size all benefit enlarging the traffic flow stable region; the traffic flow unstable region when following the car tends to fall in the relatively small distance headway range compared with that when following the truck. After that, numerical experiments demonstrate that the visual imaging information applied in VIM is more contributive to the traffic flow stability than the visual angle information in VAM when following the truck in the relatively large distance headway or involving the driver's perception threshold, i.e. Weber ratio; introducing Weber ratio would break the originally stable traffic flow or deteriorate the traffic fluctuation, which however can be alleviated by increasing drivers' sensitivity, e.g., decreasing Weber ratio. Finally, VIM is verified to be able to satisfy the consistency criteria well from the theoretical aspect.

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Ikonomidis, Alexandros, Zoe Afkou, Antonios N. Maniatis, Danai Sofianou, Athanassios Tsakris, Spyros Pournaras, and Maria Labrou. "First Occurrence of an Escherichia coli Clinical Isolate Producing the VIM-1/VIM-2 Hybrid Metallo-β-Lactamase VIM-12." Antimicrobial Agents and Chemotherapy 51, no. 8 (May 21, 2007): 3038–39. http://dx.doi.org/10.1128/aac.00374-07.

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Akano, Theddeus T. "An Explicit Solution to Continuum Compliant Cantilever Beam Problem with Various Variational Iteration Algorithms." Advanced Engineering Forum 32 (April 2019): 1–13. http://dx.doi.org/10.4028/www.scientific.net/aef.32.1.

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The geometric nonlinearity resulting from large deformation of compliant members has continued to be an interesting research topic in nonlinear mechanics. In this study, two standard variational iteration algorithms, VIM-I and VIM-III are employed to investigate the large deformation of the continuum compliant beam under point load. The VIM is an efficient technique that bypasses the linearization process and proffers solutions to nonlinear problems. The horizontal and vertical displacements of the continuum compliant cantilever beam free end are expressed in explicit analytical forms. Numerical experiment and simulations were carried out to validate the efficacy and applicability of the semi-analytical method. The VIM-I was split into two; VIM-I(A) and VIM-I(B), with the difference being the initial approximations. The results from the VIM-I(A), VIM-I(B) and VIM-III algorithms were compared with the experimental and exact solution. The outcomes reveal that both algorithms correlated well with the analytical solution and experimental result.

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King, Nicolas Kon Kam, Vibhor Krishna, Diellor Basha, Gavin Elias, Francesco Sammartino, Mojgan Hodaie, Andres M. Lozano, and William D. Hutchison. "Microelectrode recording findings within the tractography-defined ventral intermediate nucleus." Journal of Neurosurgery 126, no. 5 (May 2017): 1669–75. http://dx.doi.org/10.3171/2016.3.jns151992.

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OBJECTIVEThe ventral intermediate nucleus (VIM) of the thalamus is not visible on structural MRI. Therefore, direct VIM targeting methods for stereotactic tremor surgery are desirable. The authors previously described a direct targeting method for visualizing the VIM and its structural connectivity using deterministic tractography. In this combined electrophysiology and imaging study, the authors investigated the electrophysiology within this tractography-defined VIM (T-VIM).METHODSThalamic neurons were classified based on their relative location to the T-VIM: dorsal, within, and ventral to the T-VIM. The authors identified the movement-responsive cells (kinesthetic and tremor cells), performed spike analysis (firing rate and burst index), and local field potential analysis (area under the curve for 13–30 Hz). Tremor efficacy in response to microstimulation along the electrode trajectory was also assessed in relation to the T-VIM.RESULTSSeventy-three cells from a total of 9 microelectrode tracks were included for this analysis. Movement-responsive cells (20 kinesthetic cells and 26 tremor cells) were identified throughout the electrode trajectories. The mean firing rate and burst index of cells (n = 27) within the T-VIM are 18.8 ± 9.8 Hz and 4.5 ± 5.4, respectively. Significant local field potential beta power was identified within the T-VIM (area under the curve for 13–30 Hz = 6.6 ± 7.7) with a trend toward higher beta power in the dorsal T-VIM. The most significant reduction in tremor was also observed in the dorsal T-VIM.CONCLUSIONSThe electrophysiological findings within the VIM thalamus defined by tractography, or T-VIM, correspond with the known microelectrode recording characteristics of the VIM in patients with tremor.

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Drobinski, Patryk J., Neel I. Nissen, Dovile Sinkeviciute, Nicholas Willumsen, Morten A. Karsdal, and Anne C. Bay-Jensen. "In Contrast to Anti-CCP, MMP-Degraded and Citrullinated Vimentin (VICM) Is Both a Diagnostic and a Treatment Response Biomarker." International Journal of Molecular Sciences 24, no. 1 (December 24, 2022): 321. http://dx.doi.org/10.3390/ijms24010321.

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Protein citrullination and degradation by matrix metalloproteinases (MMP) plays a central role in the pathology of rheumatoid arthritis (RA). Autoantibodies are known to target citrullinated vimentin. The aim of this study was to investigate the relationship between the blood levels of MMP-degraded and citrullinated vimentin (VICM), as compared with the levels of MMP-degraded and non-citrullinated vimentin (VIM), and the standard anti-CCP biomarker in RA patients undergoing treatment. Thus, VIM, VICM and anti-CCP were quantified by ELISA in serum samples from baseline and week 8 of patients (n = 257) with RA, treated with either tocilizumab (8 mg/kg), methotrexate (7.5–15 mg/kg) or a placebo and compared with a reference cohort (n = 64). The three biomarkers were elevated in RA serum compared with the reference cohort: medians were 1.7 vs. 0.8 ng/mL (p < 0.05) for VIM; 7.5 vs. 0.7 ng/mL (p < 0.0001) for VICM; 57 vs. 4 RU/mL (p < 0.001) for anti-CCP. VICM was decreased in response to tocilizumab (2.9-fold, p < 0.0001) and to methotrexate (1.5-fold, p < 0.05) compared with the placebo, while anti-CCP was not. Serum VIM was also modulated by both drugs, although to a lesser degree. A high baseline level of VICM was predictive of a low disease activity response at week 8. In conclusion, VICM can differentiate between RA and healthy donors in a similar manner to anti-CCP; furthermore, VICM is also a pharmacodynamic marker.

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Yan, Jing-Jou, Po-Ren Hsueh, Wen-Chien Ko, Kwen-Tay Luh, Shu-Huei Tsai, Hsiu-Mei Wu, and Jiunn-Jong Wu. "Metallo-β-Lactamases in ClinicalPseudomonas Isolates in Taiwan and Identification of VIM-3, a Novel Variant of the VIM-2 Enzyme." Antimicrobial Agents and Chemotherapy 45, no. 8 (August 1, 2001): 2224–28. http://dx.doi.org/10.1128/aac.45.8.2224-2228.2001.

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ABSTRACT A total of 209 clinical isolates of Pseudomonas (193Pseudomonas aeruginosa, 10 P. putida, 4P. stutzeri, and 2 P. fluorescensisolates) with reduced susceptibilities to imipenem and/or ceftazidime were subjected to PCR assays with primers specific forbla IMP-1, bla IMP-2,bla VIM-1, and bla VIM-2and sequence analysis to identify the metallo-β-lactamases (MBLs) prevalent among these organisms in Taiwan; and 21 isolates gave positive results. Five isolates including two P. putida and three P. stutzeri isolates were found to carrybla IMP-1, and six isolates including fiveP. putida and one P. stutzeri isolates harboredbla VIM-2. The remaining 10 isolates wereP. aeruginosa, and all were found to carry a novel variant of bla VIM-2, designatedbla VIM-3. There are only two nucleotide differences between bla VIM-2 andbla VIM-3, leading to two amino acid alterations. Our findings indicate that VIM-2 and its variant have become the most prevalent metalloenzymes in Pseudomonas in Taiwan. Southern hybridization with thebla VIM-2-, bla VIM-3-, and bla IMP-1 -specific probes revealed that only two VIM-2-producing P. putida isolates appeared to carry the MBL gene on plasmids. Pulsed-field gel electrophoresis showed that six VIM-3-producing P. aeruginosa isolates and two IMP-1-producing P. stutzeri isolates were genetically related, suggesting that the spread of these MBL genes in Taiwan could be due to clonal dissemination as well as genetic exchange between different clones.

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Schneider, Ines, Emma Keuleyan, Rudolf Rasshofer, Rumyana Markovska, Anne Marie Queenan, and Adolf Bauernfeind. "VIM-15 and VIM-16, Two New VIM-2-Like Metallo-β-Lactamases in Pseudomonas aeruginosa Isolates from Bulgaria and Germany." Antimicrobial Agents and Chemotherapy 52, no. 8 (June 2, 2008): 2977–79. http://dx.doi.org/10.1128/aac.00175-08.

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ABSTRACT Two Pseudomonas aeruginosa urine isolates from Bulgaria and Germany produced two new VIM-2 variants. VIM-15 had one amino acid substitution (Tyr218Phe) which caused a significant increase in hydrolytic efficiency. The substitution Ser54Leu, characterizing VIM-16, showed no influence on enzyme activity. Both genes were part of class I integrons located in the chromosome.

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Robin, Frédéric, Nadjet Aggoune-Khinache, Julien Delmas, Malek Naim, and Richard Bonnet. "Novel VIM Metallo-β-Lactamase Variant from Clinical Isolates of Enterobacteriaceae from Algeria." Antimicrobial Agents and Chemotherapy 54, no. 1 (November 9, 2009): 466–70. http://dx.doi.org/10.1128/aac.00017-09.

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ABSTRACT Five different strains of bacteria belonging to the family Enterobacteriaceae were isolated from two patients hospitalized in the intensive care unit of the Central Military Hospital of Algiers, Algeria. All five strains, one Providencia stuartii strain, two Escherichia coli strains, and two Klebsiella pneumoniae strains, were intermediate or resistant to all β-lactams, including carbapenems. Synergy between imipenem and EDTA was observed for all five strains. The results of the PCR experiment confirmed the presence of a bla VIM gene in all five strains. The bla VIM genes were located as part of a class 1 integron on a 180-kb conjugative plasmid. They encoded a novel metallo-β-lactamase designated VIM-19, which differed from the parental enzyme VIM-1 by only two substitutions: Ser228Arg, previously observed in the closely related enzyme VIM-4, and Asn215Lys, not previously observed in other VIM-type carbapenemases. VIM-19 was further characterized after purification through determination of its kinetic constants. This enzyme was inhibited by EDTA and hydrolyzed penicillins, cephalosporins, and carbapenems, as observed for other VIM-type carbapenemases but with greater catalytic efficiency against penicillins than VIM-1. VIM-19 is the first carbapenemase enzyme identified from an isolate from Algeria. These results confirm the emergence of VIM-4-like enzymes in members of the family Enterobacteriaceae from Mediterranean countries.

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Wazwaz, Abdul-Majid. "Dual solutions for nonlinear boundary value problems by the variational iteration method." International Journal of Numerical Methods for Heat & Fluid Flow 27, no. 1 (January 3, 2017): 210–20. http://dx.doi.org/10.1108/hff-10-2015-0442.

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Purpose The purpose of this paper is to use the variational iteration method (VIM) for studying boundary value problems (BVPs) characterized with dual solutions. Design/methodology/approach The VIM proved to be practical for solving linear and nonlinear problems arising in scientific and engineering applications. In this work, the aim is to use the VIM for a reliable treatment of nonlinear boundary value problems characterized with dual solutions. Findings The VIM is shown to solve nonlinear BVPs, either linear or nonlinear. It is shown that the VIM solves these models without requiring restrictive assumptions and in a straightforward manner. The conclusions are justified by investigating many scientific models. Research limitations/implications The VIM provides convergent series solutions for linear and nonlinear equations in the same manner. Practical implications The VIM is practical and shows more power compared to existing techniques. Social implications The VIM handles linear and nonlinear models in the same manner. Originality/value This work highlights a reliable technique for solving nonlinear BVPs that possess dual solutions. This paper has shown the power of the VIM for handling BVPs.

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Lim, Jia Jia, Youngjin Lee, Tue Tu Ly, Jung Youn Kang, Jung-Gyu Lee, Jun Yop An, Hyung-Seop Youn, et al. "Structural insights into the interaction of p97 N-terminus domain and VBM in rhomboid protease, RHBDL4." Biochemical Journal 473, no. 18 (September 12, 2016): 2863–80. http://dx.doi.org/10.1042/bcj20160237.

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RHBDL4 is an active rhomboid that specifically recognizes and cleaves atypical, positively charged transmembrane endoplasmic reticulum-associated degradation (ERAD) substrates. Interaction of valosin-containing protein (p97/VCP) and RHBDL4 is crucial to retrotranslocate polyubiquitinated substrates for ERAD pathway. Here, we report the first complex structure of VCP-binding motif (VBM) with p97 N-terminal domain (p97N) at 1.88 Å resolution. Consistent with p97 adaptor proteins including p47-ubiquitin regulatory X (UBX), gp78-VCP-interacting motif (VIM), OTU1-UBX-like element, and FAF1-UBX, RHBDL4 VBM also binds at the interface between the two lobes of p97N. Notably, the RF residues in VBM are involved in the interaction with p97N, showing a similar interaction pattern with that of FPR signature motif in the UBX domain, although the directionality is opposite. Comparison of VBM interaction with VIM of gp78, another α-helical motif that interacts with p97N, revealed that the helix direction is inversed. Nevertheless, the conserved arginine residues in both motifs participate in the majority of the interface via extensive hydrogen bonds and ionic interactions with p97N. We identified novel VBM-binding mode to p97N that involves a combination of two types of p97–cofactor specificities observed in the UBX and VIM interactions. This highlights the induced fit model of p97N interdomain cleft upon cofactor binding to form stable p97–cofactor complexes. Our mutational and biochemical analyses in defining the specific interaction between VBM and p97N have elucidated the importance of the highly conserved VBM, applicable to other VBM-containing proteins. We also showed that RHBDL4, ubiquitins, and p97 co-operate for efficient substrate dislocation.

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Lenz, F. A., C. J. Jaeger, M. S. Seike, Y. C. Lin, and S. G. Reich. "Single-Neuron Analysis of Human Thalamus in Patients With Intention Tremor and Other Clinical Signs of Cerebellar Disease." Journal of Neurophysiology 87, no. 4 (April 1, 2002): 2084–94. http://dx.doi.org/10.1152/jn.00049.2001.

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Tremor that occurs as a result of a cerebellar lesion, cerebellar tremor, is characteristically an intention tremor. Thalamic activity may be related to cerebellar tremor because transmission of some cerebellar efferent signals occurs via the thalamus and cortex to the periphery. We have now studied thalamic neuronal activity in a cerebellar relay nucleus (ventral intermediate—Vim) and a pallidal relay nucleus (ventralis oral posterior—Vop) during thalamotomy in patients with intention tremor and other clinical signs of cerebellar disease (tremor patients). The activity of single neurons and the simultaneous electromyographic (EMG) activity of the contralateral upper extremity in tremor patients performing a pointing task were analyzed by spectral cross-correlation analysis. EMG spectra during intention tremor often showed peaks of activity in the tremor-frequency range (1.9–5.8 Hz). There were significant differences in thalamic neuronal activity between tremor patients and controls. Neurons in Vim and Vop had significantly lower firing rates in tremor patients than in patients undergoing thalamic surgery for pain (pain controls). Other studies have shown that inputs to Vim from the cerebellum are transmitted through excitatory connections. Therefore the present results suggest that tremor in these tremor patients is associated with deafferentation of the thalamus from cerebellar efferent pathways. The thalamic X EMG cross-correlation functions were studied for cells located in Vim and Vop. Neuronal and EMG activity were as likely to be significantly correlated for cells in Vim as for those in Vop. Cells in Vim were more likely to have a phase lag relative to EMG than were cells in Vop. In monkeys, cells in the cerebellar relay nucleus of the thalamus, corresponding to Vim, are reported to lead movement during active oscillations at the wrist. In view of these monkey studies, the present results suggest that cells in Vim are deafferented and have a phase lag relative to tremor that is not found in normal active oscillations. The difference in phase of thalamic spike X EMG activity between Vim and Vop may contribute to tremor because lesions of pallidum or Vop are reported to relieve cerebellar tremor.

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Schultheis, Jonathan R. "VIRUS-FREE MICROPROPAGATED PLANTS AND CLONE EFFECTS ON SWEETPOTATO YIELD, 1993." HortScience 29, no. 7 (July 1994): 727f—727. http://dx.doi.org/10.21273/hortsci.29.7.727f.

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Commercially grown sweetpotato contain virus. Hill selection is practiced to maintain quality and trueness to type of a variety. Three field plantings of Beauregard and Jewel were made in 1993 to compare the yield of virus-free planting stock obtained from micropropagared plants (VFM); virus-infected planting stock obtained from micropropagated plants (VIM); foundation, registered, grower seed stock; and a selected California Jewel clone in which the virus was removed, then micropropagated (CVFM). For Beauregard, VFM had significantly more yield of marketable and number 1 roots at the 0.06 level of significance than plants not micropropagated. The yield of number 1 roots was also greater with VFM compared with VIM. Marketable yields of Beauregard were superior when registered versus grower seed (nor in certification program) were compared. For Jewel, marketable yields were increased from VFM versus plants not micropropagated. VFM and the VIM yielded similarly as did registered and grower seed stock. The VFM Jewel clone from North Carolina outyielded CVFM. Yield was at least as good when obtained from VFM compared with the other planting stocks. A yield increase of 10 to 20% was common when using VFM, hill selected sweetpotatoes.

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27

Siarkou, Victoria I., Danai Vitti, Efthimia Protonotariou, Alexandros Ikonomidis, and Danai Sofianou. "Molecular Epidemiology of Outbreak-Related Pseudomonas aeruginosa Strains Carrying the Novel Variant blaVIM-17 Metallo-β-Lactamase Gene." Antimicrobial Agents and Chemotherapy 53, no. 4 (January 21, 2009): 1325–30. http://dx.doi.org/10.1128/aac.01230-08.

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ABSTRACT A study was designed to investigate the molecular epidemiological characteristics of multidrug-resistant outbreak-related Pseudomonas aeruginosa isolates collected in a university hospital in northern Greece. Of 29 nonreplicate P. aeruginosa isolates resistant to carbapenems and ceftazidime, 14 were positive for metallo-β-lactamase production. PCR analyses with primers specific for bla VIM and bla IMP revealed that 13 isolates carried a novel bla VIM-2 gene variant, designated bla VIM-17, and only 1 isolate carried bla VIM-2, a gene predominant among P. aeruginosa strains in Greek hospitals. Pulsed-field gel electrophoresis of XbaI-digested genomic DNAs showed a close genetic relationship for 12 of 13 bla VIM-17-carrying outbreak-related isolates, which were of the O11 serotype; the clonally unrelated isolate carrying bla VIM-17 was of the O12 serotype. PCR mapping strategies for the detection of class 1 integrons and sequencing approaches revealed the presence of integrons containing one bla VIM cassette flanked by two aacA29 cassettes. These integrons were similar but not identical to In59 (GenBank accession number AF263519) initially described in France. All isolates carrying bla VIM-17, regardless of their genetic profile, had an identical integron, named In59.3, indicating that although the hospital outbreak was mainly due to clonal dissemination, the horizontal transmission of the bla VIM-17-containing integron among P. aeruginosa isolates should also have occurred. An outbreak-related isolate and a control strain, both of which carried the bla VIM-2 gene but which were clonally distinct, had an identical integron, named In59.2, which differed only at the level of the bla VIM gene from In59.3 integrons, suggesting a common ancestry. The spread of the bla VIM-17-containing integron in clonally unrelated P. aeruginosa isolates without any evidence of plasmid carriage is probably associated with a transposon.

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Menezes, Krishe, Milind Deogaonkar, and Vatsal Bajpai. "Vim stimulation as a predictor of response to deep brain stimulation in patients of severe tremor undergoing dual stimulation." Translation: The University of Toledo Journal of Medical Sciences 2 (June 27, 2018): 7–10. http://dx.doi.org/10.46570/utjms.vol2-2015-103.

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Deep brain stimulation, targeting the ventral intermediate nucleus of the thalamus (Vim), has been shown to be an effective management tool for tremors refractory to other therapies. There is some variance in response to Vim stimulation for severe essential and rubral tremors. This study looked at dual stimulations (addition, in which the Vim is stimulated along with an additional nucleus or augmentation, in which a second lead is placed within the Vim itself) for these types of tremors. A total of eight patients, four with rubral and four with severe essential tremors, were treated with deep brain stimulation. The responses of the patients were characterized on a scale from excellent improvement to worsening of condition. Two of the four patients with rubral tremor had an excellent response to Vim stimulation. These patients showed additional benefits when the prelemniscal radiation (Raprl) was stimulated, in addition to the Vim. Three of the four patients with severe essential tremor reported either a good or excellent response to Vim stimulation. One of these patients had the Raprl stimulated in addition to the Vim while another had an augmentation of the Vim, with ventralis oralis posterior (Vop) stimulation. Both showed additional benefits with the addition or augmentation performed. We conclude that if a patient with severe medically refractory tremor (essential or rubral tremor) responds to Vim stimulation but is still disabled he will likely also have a response to dual stimulation with an additional lead in the Raprl or an augmentation with an additional lead in the Vop. Patients who did not initially respond to Vim stimulation did not respond to the placement of a second lead. We also conclude that for severe essential tremor, Raprl stimulation showed a better response than Vim stimulation.

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Torabi, Mohsen, and Hessameddin Yaghoobi. "Two dominant analytical methods for thermal analysis of convective step fin with variable thermal conductivity." Thermal Science 18, no. 2 (2014): 431–42. http://dx.doi.org/10.2298/tsci110918046t.

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Heat transfer in a straight fin with a step change in thickness and variable thermal conductivity which is losing heat by convection to its surroundings is developed via differential transformation method (DTM) and variational iteration method (VIM). In this study, we compare DTM and VIM results, with those of homotopy perturbation method (HPM) and an accurate numerical solution to verify the accuracy of the proposed methods. As an important result, it is depicted that the DTM results are more accurate in comparison with those obtained by VIM and HPM. After these verifications the effects of parameters such as thickness ratio, ?, dimensionless fin semi thickness,?, length ratio, ?, thermal conductivity parameter, ?, Biot number, Bi, on the temperature distribution are illustrated and explained.

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Bogaerts, Pierre, Carine Bebrone, Te-Din Huang, Warda Bouchahrouf, Yves DeGheldre, Ariane Deplano, Kurt Hoffmann, and Youri Glupczynski. "Detection and Characterization of VIM-31, a New Variant of VIM-2 with Tyr224His and His252Arg Mutations, in a Clinical Isolate of Enterobacter cloacae." Antimicrobial Agents and Chemotherapy 56, no. 6 (March 5, 2012): 3283–87. http://dx.doi.org/10.1128/aac.06249-11.

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ABSTRACTWe report the first description of the metallo-β-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, inEnterobacter cloacae11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium.blaVIM-31was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lowerkcat(except for ertapenem) and higherKmvalues for VIM-31.

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Zhang, Yan, Qiaoling Chen, Fujuan Liu, and Ping Wang. "The estimation of the length constant of a long cooling fin by variational iteration method." International Journal of Numerical Methods for Heat & Fluid Flow 25, no. 4 (May 5, 2015): 887–91. http://dx.doi.org/10.1108/hff-05-2014-0153.

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Purpose – The purpose of this paper is to validate the variational iteration method (VIM) is suitable for various nonlinear equations. Design/methodology/approach – The He’s VIM is applied to solve nonlinear equation which is derived from actual engineering problem. The result was compared with other method. Findings – The result obtained from VIM shows good agreement with Xu’s result which provide a solid evidence that VIM is convenient and effective for solving nonlinear equation in the engineering. Originality/value – The VIM can be extended to many academic and engineering fields for nonlinear equations solving.

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Yatsuyanagi, Jun, Shioko Saito, Seizaburo Harata, Noriyuki Suzuki, Yuko Ito, Ken-ichi Amano, and Katsuhiko Enomoto. "Class 1 Integron Containing Metallo-β-Lactamase Gene blaVIM-2 in Pseudomonas aeruginosa Clinical Strains Isolated in Japan." Antimicrobial Agents and Chemotherapy 48, no. 2 (February 2004): 626–28. http://dx.doi.org/10.1128/aac.48.2.626-628.2004.

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ABSTRACT Four bla VIM-2 gene-harboring Pseudomonas aeruginosa strains were identified. These strains possessed a class 1 integron harboring ORF1, bla VIM-2, and aacA4 gene cassettes. The transposon-mediated horizontal spread of the bla VIM-2 gene among these strains was suggested, which increases the threat that the bla VIM-2 gene will disseminate among diverse genera of bacteria.

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Korpus, R. A., and S. Liapis. "Active and Passive Control of Spar Vortex-Induced Motions." Journal of Offshore Mechanics and Arctic Engineering 129, no. 4 (February 9, 2007): 290–99. http://dx.doi.org/10.1115/1.2746400.

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Spars have become an “industry solution” for deepwater developments. Vortex-induced motion (VIM) of spar platforms in currents remains an important design concern. Although strakes are effective at suppressing riser VIM, all three straked classical spars in the Gulf of Mexico have experienced significant VIM events. These are not examples of poor design but indicate a lack of adequate tools for predicting spar VIM. This paper presents the development and validation of unsteady Reynolds-averaged Navier-Stokes (URANS) methods to predict real-world spar VIM behavior. It includes the ability to address rough surfaces and high supercritical Reynolds numbers. The resulting algorithms are used to assess the effectiveness of active and passive control strategies for suppressing spar VIM. Active control consists of injecting high-pressure water tangentially into the boundary layer and is shown to be extremely effective at reducing drag and VIM amplitudes. Passive control utilizes a sleeve to channel high-pressure stagnation flow into the boundary layer and is found less effective.

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Lin, L. S., G. W. Levan, S. M. Russell, and C. C. Law. "Effect of hafnium addition on a Ni-Al-Co Alloy." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 4 (August 1990): 940–41. http://dx.doi.org/10.1017/s0424820100177830.

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Recent efforts at P&W have shown that the addition of cobalt to binary NiAl results in an appreciable increase in room temperature ductility. One version of this ternary alloy, designated VIM A, has a composition of Ni-30 at.% Al-35 at.% Co. The addition of 0.5 at.% Hf to this alloy (designated VIM AH) results in an improvement in yield strength at 760°C. Room temperature properties were not found to be significantly affected by the Hf addition. This discussion will focus on the microstructures of alloys VIM A and VIM AH and their relationship to the mechanical properties observed in compression at room temperature and 760°C.The addition of hafnium reduced the grain size of VIM AH alloy. After room temperature compression, both alloys show an ordered bcc (B2) matrix and precipitates which are distributed primarily along grain boundaries. These precipitates were identified by microdiffraction to be ordered fcc (L12) gamma prime for VIM A and hexagonal (A3) for VIM AH.

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Gillard, B. K., L. T. Thurmon, R. G. Harrell, Y. Capetanaki, M. Saito, R. K. Yu, and D. M. Marcus. "Biosynthesis of glycosphingolipids is reduced in the absence of a vimentin intermediate filament network." Journal of Cell Science 107, no. 12 (December 1, 1994): 3545–55. http://dx.doi.org/10.1242/jcs.107.12.3545.

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Our previous observations on the immunocytochemical colocalization of intermediate filaments and glycosphingolipids led us to analyze the role of filaments in the biosynthesis and intracellular transport of glycosphingolipids. Cells with (vim+) and without (vim-) vimentin intermediate filaments were cloned from the adrenal carcinoma cell line SW13. There was no difference between vim+ and vim- cells in the proportion of newly synthesized C6-NBD-glucosylceramide transported to the plasma membrane. The vim+ cells synthesized glycosphingolipids, especially lactosylceramide and globotriosylceramide, and to a lesser extent GM3 ganglioside, more rapidly than vim- cells. The altered rate of biosynthesis did not result from differences in the levels of the glycosyltransferases that synthesize those compounds. To determine whether the presence of a vimentin network was responsible for the differences in biosynthesis, mouse vimentin cDNA was transfected into vim- cells. Transfected cells that expressed a mouse vimentin network demonstrated a twofold or greater increase in the rate of biosynthesis of neutral glycosphingolipids and gangliosides. There was no difference between vim+ and vim- cells in the synthesis of ceramide or sphingomyelin, or in their content of phospholipids or cholesterol. The nature of the biochemical defect(s) underlying the diminished incorporation of radiolabeled sugars into glycosphingolipids is unclear. Possibilities include alterations in the ultrastructure of the Golgi and/or abnormalities in a portion of the endocytic pathway.

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Katsarou, Spyridoula D., Ippokratis Messaritakis, Anastasia Voumvouraki, Stavros Kakavogiannis, Athanasios Κotsakis, Saad Alkahtani, Christos Stournaras, Stuart S. Martin, Vassilis Georgoulias, and Galatea Kallergi. "Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients." Journal of Personalized Medicine 12, no. 2 (January 25, 2022): 154. http://dx.doi.org/10.3390/jpm12020154.

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Upregulation of Vimentin (VIM), alpha-Tubulin (TUB) and Detyrosinated tubulin (GLU) in circulating tumor cells (CTCs) derived from breast cancer patients is related to poor prognosis. In the current study we evaluated for the first time, these cytoskeletal proteins in sixty Non-Small Cell Lung Cancer (NSCLC) patients’ CTCs (33 treatment-naïve and 27 pre-treated). Samples were isolated using the ISET platform and stained with a pancytokeratin (CK)/CD45/TUB, CK/GLU/VIM and CK/programmed death ligand 1 (PD-L1) combination of antibodies. Subsequently, slides were analyzed using confocal laser scanning microscopy. CTCs were detected in 86.7% of the patients. CTCs with TUB expression were identified in 65.4% (34/52) of the CK (+)-patients. GLU, VIM and PD-L1 were also evaluated. The frequency of the observed phenotypes was as follow: (CK+/GLU−/VIM−): 35.2%, (CK+/GLU+/VIM+): 63.0%, (CK+/GLU+/VIM−): 16.7%, (CK+/GLU−/VIM+): 72.2%, (CK+/PD-L1−): 75% and (CK+/PD-L1+): 55%. The OS was significantly decreased in patients with high GLU (3.8 vs. 7.9 months; p = 0.018) and/or high VIM (3.2 vs. 7.1 months; p = 0.029) expression in their CTCs. PD-L1 was also related to OS (3.4 vs. 7.21 months; p = 0.035). Moreover, TUB-high and TUB-low expression in CTCs inversely influenced patients’ OS as independent prognostic factors (p = 0.041 and p = 0.009). The current study revealed that TUB, GLU, VIM and PD-L1 were overexpressed in CTCs from NSCLC patients. Furthermore, the presence of GLU, VIM-positive and PD-L1 in CTCs is potentially related to patients’ outcomes.

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Tato, Marta, Teresa M. Coque, Fernando Baquero, and Rafael Cantón. "Dispersal of Carbapenemase blaVIM-1 Gene Associated with Different Tn402 Variants, Mercury Transposons, and Conjugative Plasmids in Enterobacteriaceae and Pseudomonas aeruginosa." Antimicrobial Agents and Chemotherapy 54, no. 1 (November 9, 2009): 320–27. http://dx.doi.org/10.1128/aac.00783-09.

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ABSTRACT The emergence of bla VIM-1 within four different genetic platforms from distinct Enterobacteriaceae and Pseudomonas aeruginosa isolates in an area with a low prevalence of metallo-β-lactamase producers is reported. Forty-three VIM-1-producing isolates (including 19 Enterobacter cloacae, 2 Escherichia coli, and 2 P. aeruginosa isolates, 18 Klebsiella pneumoniae isolate, and 2 Klebsiella oxytoca isolate) recovered from 2005 to 2007 and corresponding to 15 pulsed-field gel electrophoresis types were studied. The Enterobacteriaceae isolates corresponded to a hospital outbreak, and the P. aeruginosa isolates were sporadically recovered. The genetic context of the integrons carrying bla VIM-1 (arbitrarily designated types A, B, C, and D) was characterized by PCR mapping based on known Tn402 and mercury transposons and further sequencing. Among Enterobacteriaceae isolates, bla VIM-1 was part of integrons located either in an In2-Tn402 element linked to Tn21 (type A; In110-bla VIM-1-aacA4-aadA1) or in a Tn402 transposon lacking the whole tni module [type B; In113-bla VIM-1-aacA4-dhfrII (also called dfrB1)-aadA1-catB2] and the transposon was associated with an IncHI2 or IncI1 plasmid, respectively. Among P. aeruginosa isolates, bla VIM-1 was part of a new gene cassette array located in a defective Tn402 transposon carrying either tniBΔ3 and tniA (type C; bla VIM-1-aadA1) or tniC and ΔtniQ (type D; bla VIM-1-aadB), and both Tn402 variants were associated with conjugative plasmids of 30 kb. The dissemination of bla VIM-1 was associated with different genetic structures and bacterial hosts, depicting a complex emergence and evolutionary network scenario in our facility, Ramón y Cajal University Hospital, Madrid, Spain. Knowledge of the complex epidemiology of bla VIM-1 is necessary to control this emerging threat.

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Schwarzinger, I., P. Valent, U. Köller, C. Marosi, B. Schneider, O. Haas, W. Knapp, K. Lechner, and P. Bettelheim. "Prognostic significance of surface marker expression on blasts of patients with de novo acute myeloblastic leukemia." Journal of Clinical Oncology 8, no. 3 (March 1990): 423–30. http://dx.doi.org/10.1200/jco.1990.8.3.423.

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The prognostic significance of the expression of surface membrane antigens on the blasts of 123 consecutive patients with de novo acute myeloblastic leukemia (AML) was evaluated. For this purpose, reactivity of monoclonal antibodies (mAbs) CLB-ERY3 (antiblood-group H antigen), VIM-D5 (CD15), WT1 (CD7), MY7 (CD13), MY9 (CD33), VID-1 (antihuman leukocyte antigen locus DR [anti-HLA DR]), VIM-2 (CDw65L), VIM-13 (CD14), 63D3 (CD14) and anti-TdT with leukemic blast cell populations was prospectively analyzed with respect to the rates of complete remission (CR), continuous complete remission (CCR), and survival. The overall rate of CR was 65%, the 6-year rates of overall CCR and survival were 23% and 13%, respectively (median period of patient observation, 30 months). Of all Abs tested, four (CLB-ERY3, MY7, anti-TdT, and VIM-D5) were found to be of prognostic value. Reactivity of CLB-ERY3, MY7, and anti-TdT was predictive for CR (CLB-ERY3+, 43% v CLB-ERY3-, 73%, P less than .02; MY7+, 59% v MY7-, 91%, P less than .003; TdT+, 28% v TdT-, 71%, P less than .001, respectively) and probability of survival (significantly lower survival rates: CLB-ERY3+, P less than .02; MY7+, P less than .03; and TdT+ cases, P less than .001, respectively). Reactivity of VIM-D5 was significantly associated with a higher probability of CCR (P less than .01). Our results confirm earlier reports on the prognostic significance of expression of CD13 and TdT in AML and indicate CLB-ERY3 (antiblood-group H antibody) and VIM-D5 (CD15) as further markers predictive for the clinical outcome in patients with de novo AML.

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Ranjan, Manish, Gavin J. B. Elias, Alexandre Boutet, Jidan Zhong, Powell Chu, Jurgen Germann, Gabriel A. Devenyi, et al. "Tractography-based targeting of the ventral intermediate nucleus: accuracy and clinical utility in MRgFUS thalamotomy." Journal of Neurosurgery 133, no. 4 (October 2020): 1002–9. http://dx.doi.org/10.3171/2019.6.jns19612.

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OBJECTIVETractography-based targeting of the thalamic ventral intermediate nucleus (T-VIM) is a novel method conferring patient-specific selection of VIM coordinates for tremor surgery; however, its accuracy and clinical utility in magnetic resonance imaging–guided focused ultrasound (MRgFUS) thalamotomy compared to conventional indirect targeting has not been specifically addressed. This retrospective study sought to compare the treatment locations and potential adverse effect profiles of T-VIM with indirect targeting in a large cohort of MRgFUS thalamotomy patients.METHODST-VIM was performed using diffusion tractography outlining the pyramidal and medial lemniscus tracts in 43 MRgFUS thalamotomy patients. T-VIM coordinates were compared with the indirect treatment coordinates used in the procedure. Thalamotomy lesions were delineated on postoperative T1-weighted images and displaced (“translated”) by the anteroposterior and mediolateral difference between T-VIM and treatment coordinates. Both translated and actual lesions were normalized to standard space and subsequently overlaid with areas previously reported to be associated with an increased risk of motor and sensory adverse effects when lesioned during MRgFUS thalamotomy.RESULTST-VIM coordinates were 2.18 mm anterior and 1.82 mm medial to the “final” indirect treatment coordinates. Translated lesions lay more squarely within the boundaries of the VIM compared to nontranslated lesions and showed significantly less overlap with areas associated with sensory adverse effects. Translated lesions overlapped less with areas associated with motor adverse effects; however, this difference was not significant.CONCLUSIONST-VIM leads to the selection of more anterior and medial coordinates than the conventional indirect methods. Lesions moved toward these anteromedial coordinates avoid areas associated with an increased risk of motor and sensory adverse effects, suggesting that T-VIM may improve clinical outcomes.

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Vázquez, Elizabeth, Claudia Muro, Javier Illescas, Guillermina Burillo, Omar Hernández, and Ernesto Rivera. "Obtainment and Characterization of Hydrophilic Polysulfone Membranes by N-Vinylimidazole Grafting Induced by Gamma Irradiation." Polymers 12, no. 6 (June 4, 2020): 1284. http://dx.doi.org/10.3390/polym12061284.

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Polysulfone (PSU) film and N-vinylimidazole (VIM) were used to obtain grafted membranes with high hydrophilic capacity. The grafting process was performed by gamma irradiation under two experiments: (1) different irradiation doses (100–400 kGy) and VIM 50% solution; (2) different concentration of grafted VIM (30–70%) and 300 kGy of irradiation dose. Characteristics of the grafted membranes were determined by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), contact angle, swelling degree, desalination test, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Both experiments indicated that the absorbed dose 300 kGy and the VIM concentration, at 50% v/v, were effective to obtain PSU grafted membranes with 14.3% of grafting yield. Nevertheless, experimental conditions, 400 kGy, VIM 50% and 300 kGy, VIM 60–70% promoted possible membrane degradation and VIM homopolymerization on the membrane surface, which was observed by SEM images; meanwhile, 100–200 kGy and VIM 30–50% produced minimal grafting (2 ± 0.5%). Hydrophilic surface of the grafted PSU membranes by 300 kGy and VIM 50% v/v were corroborated by the water contact angle, swelling degree and desalination test, showing a decrease from 90.7° ± 0.3 (PSU film) to 64.3° ± 0.5; an increment of swelling degree of 25 ± 1%, and a rejection-permeation capacity of 75 ± 2%. In addition, the thermal behavior of grafted PSU membranes registered an increment in the degradation of 20%, due to the presence of VIM. However, the normal temperature of the membrane operation did not affect this result; meanwhile, the glass transition temperature (Tg) of the grafted PSU membrane was found at 185.4 ± 0.5 °C, which indicated an increment of 15 ± 1%.

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Zhu, Huimin, Yujuan Cao, Weitao Su, Shan Huang, Weizhi Lu, Yezhen Zhou, Jing Gao, Wei Zhao, Bao Zhang, and Xianbo Wu. "Enterovirus A71 VP1 Variation A289T Decreases the Central Nervous System Infectivity via Attenuation of Interactions between VP1 and Vimentin In Vitro and In Vivo." Viruses 11, no. 5 (May 22, 2019): 467. http://dx.doi.org/10.3390/v11050467.

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Vimentin (VIM) is a surface receptor for enterovirus-A71, mediating the initial binding and subsequent increase in EV-A71 infectivity. The caspid protein VP1 variation, A289T, is reportedly closely associated with less severe central nervous system (CNS) infections in humans. However, it is unclear whether VIM is associated with a reduction in CNS infections of EV-A71 in the presence of A289T. We investigated whether VIM served as a receptor for EV-A71 in the presence of an A298T substitution in VP1. EV-A71-289A and EV-A71-289T were used to infect human rhabdomyosarcoma cells, control human brain microvascular endothelial cells (HBMECs), and VIM-knockout (KO) HBMECs and inoculated BALB/c mice, SV129 mice, and VIM-KO SV129 mice. Furthermore, we cloned VP1-289A-Flag and VP1-289T-Flag proteins for co-immunoprecipitation analysis. Analysis of viral function revealed that the capacity of viral attachment, replication, and protein synthesis and secretion decreased in HBMECs during an EV-A71-289A infection, the infectivity being higher than that of EV-A71-289T upon VIM-KO. Histopathological and immunohistochemical analyses of brain tissue revealed that cerebral cortical damage was more extensive in EV-A71-289A than in EV-A71-289T infections in control SV129 mice; however, no significant difference was observed upon VIM-KO. Co-immunoprecipitation analysis revealed an interaction between VP1 and VIM, which was attenuated in VP1 harboring A289T; however, this attenuation was reversed by VIM (1-58) peptide. The A289T variation of VP1 specifically decreased the virulence of EV-A71 in HBMECs, and the attenuated interaction between VP1 harboring the A289T variation and VIM essentially decreased the CNS infectivity of EV-A71 in vitro and vivo.

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42

Sarria, A. J., J. G. Lieber, S. K. Nordeen, and R. M. Evans. "The presence or absence of a vimentin-type intermediate filament network affects the shape of the nucleus in human SW-13 cells." Journal of Cell Science 107, no. 6 (June 1, 1994): 1593–607. http://dx.doi.org/10.1242/jcs.107.6.1593.

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Human SW-13 cells express the intermediate filament protein vimentin in a mosaic pattern (Hedberg, K. K. and Chen, L. B. (1986). Exp. Cell Res. 163, 509–517). We have isolated SW-13 clones that do (vim+) or do not (vim-) synthesize vimentin as analyzed using anti-intermediate filament immunofluorescence, electron microscopy and two-dimensional gel analysis of detergent-extracted preparations. Vimentin is the only cytoplasmic intermediate filament protein present in the vim+ cells, and the vim- cells do not contain any detectable cytoplasmic intermediate filament system. The presence or absence of intermediate filaments did not observably affect the distribution of mitochondria, endoplasmic reticulum, microtubules or actin stress fibers when these structures were visualized by fluorescence microscopy. However, electron microscopy and anti-lamin A/C immunofluorescence studies showed that nuclear morphology in vim- cells was frequently characterized by large folds or invaginations, while vim+ cells had a more regular or smooth nuclear shape. When vim- cells were transfected with a mouse vimentin expression plasmid, the synthesis of a mouse vimentin filament network restored the smooth nuclear morphology characteristic of vim+ cells. Conversely, when vim+ cells were transfected with a carboxy-terminally truncated mutant vimentin, expression of the mutant protein disrupted the organization of the endogenous vimentin filaments and resulted in nuclei with a prominently invaginated morphology. These results indicated that in SW-13 cells the vimentin filament system affects the shape of the nucleus.

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43

Moreno, Juan Carlos Alvarez, Hisham F. Bahmad, Christopher A. Febres-Aldana, Andrés Pirela, Andres Azuero, Ali Salami, and Robert Poppiti. "Post-mortem assessment of vimentin expression as a biomarker for renal tubular regeneration following acute kidney injury." Journal of Pathology and Translational Medicine 55, no. 6 (November 15, 2021): 369–79. http://dx.doi.org/10.4132/jptm.2021.08.03.

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Background: Acute kidney injury (AKI) is a common cause of morbidity and mortality. It mainly targets the renal tubular epithelium with pathological changes, referred to as acute tubular injury. The latter is followed by a regenerative response that is difficult to visualize on routine hematoxylin and eosin (H&E) stains. In this study, we examined the regenerative capacity of renal tubules by correlating vimentin (VIM) immunohistochemical (IHC) expression and pathological findings of AKI and renal tubular regeneration (RTR) on H&E.Methods: We reviewed 23 autopsies performed in the clinical setting of AKI and RTR. VIM expression was scored in the renal cortical tubular epithelium using a statistical cutoff ≥ 3% for high expression and < 3% for low expression.Results: Of the 23 kidney tissues examined, seven (30.4%) had low VIM expression, and 16 (69.6%) had high VIM expression. Kidney tissues with evidence of AKI and RTR had significantly higher VIM expression. Renal peritubular microenvironment features showing regenerative changes on H&E were associated with high VIM expression. In the univariate model, kidney tissues with RTR were 18-fold more likely to have high VIM expression.Conclusions: In conclusion, our findings suggest that VIM could serve as an IHC marker for RTR following AKI. However, correlation with H&E findings remains critical to excluding chronic tubular damage. Collectively, our preliminary results pave the way for future studies including a larger sample size to validate the use of VIM as a reliable biomarker for RTR.

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Rodriguez-Martinez, Jose-Manuel, Patrice Nordmann, Nicolas Fortineau, and Laurent Poirel. "VIM-19, a Metallo-β-Lactamase with Increased Carbapenemase Activity from Escherichia coli and Klebsiella pneumoniae." Antimicrobial Agents and Chemotherapy 54, no. 1 (November 16, 2009): 471–76. http://dx.doi.org/10.1128/aac.00458-09.

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ABSTRACT Two carbapenem-resistant isolates, one Escherichia coli isolate and one Klebsiella pneumoniae isolate, recovered from an Algerian patient expressed a novel VIM-type metallo-β-lactamase (MBL). The identified bla VIM-19 gene was located on a ca. 160-kb plasmid and located inside a class 1 integron in both isolates. VIM-19 differed from VIM-1 by the Asn215Lys and Ser228Arg substitutions, increasing its hydrolytic activity toward carbapenems. Site-directed mutagenesis experiments showed that both substitutions were necessary for the increased carbapenemase activity of VIM-19. This study indicates that MBLs with enhanced activity toward carbapenems may be obtained as a result of very few amino acid substitutions.

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45

Lozano, Andrés M. "Vim Thalamic Stimulation for Tremor." Archives of Medical Research 31, no. 3 (May 2000): 266–69. http://dx.doi.org/10.1016/s0188-4409(00)00081-3.

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46

Lamont, Richard J. "Controlling Porphyromonas gingivalis requires Vim." Microbiology 156, no. 7 (July 1, 2010): 1907–8. http://dx.doi.org/10.1099/mic.0.041251-0.

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47

Halpern, M. "VIM: Taming software with hardware." Computer 36, no. 10 (October 2003): 21–25. http://dx.doi.org/10.1109/mc.2003.1236467.

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48

Bright, J. G., and K. J. Johnston. "Whither VIM? — A developer's view." European Journal of Operational Research 54, no. 3 (October 1991): 357–62. http://dx.doi.org/10.1016/0377-2217(91)90111-8.

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Papagiannitsis, C. C., S. D. Kotsakis, E. Petinaki, A. C. Vatopoulos, E. Tzelepi, V. Miriagou, and L. S. Tzouvelekis. "Characterization of Metallo-β-Lactamase VIM-27, an A57S Mutant of VIM-1 Associated with Klebsiella pneumoniae ST147." Antimicrobial Agents and Chemotherapy 55, no. 7 (April 25, 2011): 3570–72. http://dx.doi.org/10.1128/aac.00238-11.

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ABSTRACTVIM-27 metallo-β-lactamase, an Ala57→ Ser variant of VIM-1, was identified in threeKlebsiella pneumoniaeisolates belonging to sequence type 147.blaVIM-27was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.

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Foote, Kelly D., Paul Seignourel, Hubert H. Fernandez, Janet Romrell, Elaine Whidden, Charles Jacobson, Ramon L. Rodriguez, and Michael S. Okun. "Dual Electrode Thalamic Deep Brain Stimulation For The Treatment Of Posttraumatic And Multiple Sclerosis Tremor." Operative Neurosurgery 58, suppl_4 (April 1, 2006): ONS—280—ONS—286. http://dx.doi.org/10.1227/01.neu.0000192692.95455.fd.

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Abstract Objective: To report the results of ventralis intermedius nucleus/ventralis oralis posterior nucleus (VIM) plus ventralis oralis anterior (VOA)/ventralis oralis posterior (VOP) thalamic deep brain stimulation (DBS) for the treatment of posttraumatic and multiple sclerosis tremor. Objective: The treatment of posttraumatic tremor and multiple sclerosis tremor, by either medication or surgery, has proven difficult. Lesions and DBS have had mixed and somewhat disappointing results. Previously, we reported the use of two DBS electrodes (one at the VIM/VOP border and one at the VOA/VOP border) as effective for the treatment of posttraumatic tremor in a single patient. In this study, we report the results of this technique on four patients. Methods: Four patients with either posttraumatic tremor (n = 3) or multiple sclerosis tremor (n = 1) underwent placement of two DBS electrodes (one at the VIM/VOP border and one at the VOA/VOP border). Patients underwent preoperative testing and testing at a minimum of 6 months after implantation in four conditions: On VIM DBS/On VOA/VOP DBS; On VIM DBS/Off VOA VOP DBS (5 h DBS washout); Off VIM DBS/Off VOA/VOP DBS (12 h overnight washout); and Off VIM DBS/On VOA/ VOP DBS (5 h DBS washout). Results: Each of the patients showed improvements in all four conditions when compared with the baseline. All of the improvements were maintained with chronic DBS, without tremor rebound. An analysis was performed to determine whether each condition was associated with symptom reduction (percentage change). The percentage reduction was significant for each condition and measure, despite the small number of participants. For the total tremor rating scale score, the Off VIM/Off VOA/VOP condition yielded less symptom reduction than the On VIM condition or the On VOA/VOP condition. The On VIM and On VOA/VOP conditions did not differ significantly from each other in terms of contralateral upper extremity symptoms or total clinical score. Activation of both the VIM and VOA/VOP electrodes was associated with the greatest symptom reduction. Conclusion: Tremors, such as those examined in this study, that are refractory to medications and have a poor response to VIM DBS monotherapy, may respond favorably to VIM plus VOA/VOP DBS. Two electrodes may be better than one for the treatment of certain disorders; however, more study will be required to confirm this hypothesis.

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Journal articles on the topic 'VIM'

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